##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938466_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2259215 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.762402427391816 31.0 31.0 34.0 30.0 34.0 2 31.987717415119853 31.0 31.0 34.0 30.0 34.0 3 32.05807592460213 33.0 31.0 34.0 30.0 34.0 4 35.73585692375449 37.0 35.0 37.0 33.0 37.0 5 35.598209112457205 37.0 35.0 37.0 33.0 37.0 6 35.62132510628692 37.0 35.0 37.0 33.0 37.0 7 35.555288009330674 37.0 35.0 37.0 33.0 37.0 8 35.54069444475183 37.0 35.0 37.0 33.0 37.0 9 37.1199434316787 39.0 37.0 39.0 33.0 39.0 10-11 37.02526629824961 39.0 37.0 39.0 33.0 39.0 12-13 36.92174959001246 39.0 37.0 39.0 33.0 39.0 14-15 37.983424773649254 40.0 37.0 41.0 33.0 41.0 16-17 37.90277530027022 40.0 37.0 41.0 33.0 41.0 18-19 38.00062765164006 40.0 37.0 41.0 33.0 41.0 20-21 38.04094408898666 40.0 37.0 41.0 33.0 41.0 22-23 37.95517270379313 40.0 37.0 41.0 33.0 41.0 24-25 37.85816954119019 40.0 37.0 41.0 32.0 41.0 26-27 37.669267422533935 40.0 37.0 41.0 32.0 41.0 28-29 37.55135057973676 39.5 36.5 41.0 32.0 41.0 30-31 37.31402168452317 39.0 36.0 41.0 31.0 41.0 32-33 37.10267482289203 39.0 36.0 41.0 31.0 41.0 34-35 36.958358323576995 39.0 36.0 41.0 30.5 41.0 36-37 36.958435784110854 39.0 36.0 41.0 30.5 41.0 38-39 36.83709629229622 39.0 35.5 41.0 30.0 41.0 40-41 36.66251906082422 39.0 35.0 41.0 30.0 41.0 42-43 36.442947218392234 39.0 35.0 40.0 30.0 41.0 44-45 36.20204739256777 39.0 35.0 40.0 30.0 41.0 46-47 35.86470123472091 38.0 35.0 40.0 28.5 41.0 48-49 35.527594540581575 38.0 34.5 40.0 27.5 41.0 50-51 35.16852446535633 38.0 34.0 40.0 26.5 41.0 52-53 34.67759642176597 38.0 33.0 40.0 26.0 41.0 54-55 34.36246328923984 38.0 33.0 40.0 24.5 41.0 56-57 34.02835121933946 37.0 33.0 40.0 24.0 41.0 58-59 33.5849857140644 37.0 32.0 39.5 23.0 41.0 60-61 33.21507072146741 36.0 32.0 39.0 22.5 40.5 62-63 32.754417131614304 36.0 31.0 39.0 21.5 40.0 64-65 32.24731621381763 35.0 31.0 38.5 20.0 40.0 66-67 31.770184333939 35.0 30.5 38.0 18.5 40.0 68-69 31.232460168686913 34.5 29.5 38.0 17.0 40.0 70-71 30.65171287371941 34.0 29.0 37.0 13.0 39.0 72-73 30.209669951731023 34.0 29.0 36.0 9.5 39.0 74-75 29.69673492783998 34.0 28.5 36.0 7.5 39.0 76-77 28.44758223542248 31.5 26.5 34.5 7.0 36.5 78-79 29.14191964908165 33.5 28.5 35.0 6.5 37.0 80-81 28.874792571756117 33.5 28.0 35.0 2.0 37.0 82-83 28.399024439905013 33.0 27.0 35.0 2.0 36.0 84-85 27.876409947703074 33.0 26.0 35.0 2.0 36.0 86-87 27.3054029828945 32.5 26.0 35.0 2.0 35.5 88-89 26.657194423726825 32.0 25.0 34.0 2.0 35.0 90-91 26.11658872661522 32.0 24.0 34.0 2.0 35.0 92-93 25.387103042428453 31.0 21.5 34.0 2.0 35.0 94-95 24.616555086611942 31.0 18.5 34.0 2.0 35.0 96-97 23.620563779896997 31.0 4.5 34.0 2.0 35.0 98-99 22.38544450174065 30.5 2.0 34.0 2.0 35.0 100-101 18.73338571140861 24.0 2.0 32.0 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21940640000533662 1101 2 0.2397402995245983 1101 3 0.29069951052626664 1101 4 0.1903817132166239 1101 5 0.011831339743437752 1101 6 0.13948833234126568 1101 7 0.10063770717748355 1101 8 0.1887649190808247 1101 9 0.22631629199496217 1101 10-11 0.23075952434346192 1101 12-13 0.14446495324474284 1101 14-15 0.2639409411128071 1101 16-17 0.265360397421496 1101 18-19 0.34039998465169674 1101 20-21 0.31048930210572934 1101 22-23 0.2683794827404924 1101 24-25 0.30508391477522423 1101 26-27 0.39753285696887986 1101 28-29 0.22411833330482978 1101 30-31 0.19259323009584506 1101 32-33 0.29413337156340447 1101 34-35 0.2506806493230229 1101 36-37 0.45187107675793214 1101 38-39 0.4768969847175626 1101 40-41 0.3192065876458372 1101 42-43 0.3217881003204752 1101 44-45 0.42661966524300254 1101 46-47 0.4633193620341203 1101 48-49 0.3074267624037006 1101 50-51 0.46163655653596436 1101 52-53 0.6291386722039363 1101 54-55 0.4174211515667352 1101 56-57 0.5690048151026872 1101 58-59 0.5871305360751293 1101 60-61 0.5964354308106508 1101 62-63 0.47799476326096624 1101 64-65 0.42014143725708664 1101 66-67 0.44213095000054636 1101 68-69 0.5273921468072693 1101 70-71 0.4114308436534806 1101 72-73 0.4157990839416037 1101 74-75 0.3376453591172499 1101 76-77 0.36184869128700825 1101 78-79 0.3802477325030367 1101 80-81 0.5188526741273769 1101 82-83 0.36737526185742553 1101 84-85 0.5180576229631946 1101 86-87 0.4534296823936259 1101 88-89 0.57876425602268 1101 90-91 0.5101747834767956 1101 92-93 0.756401792353774 1101 94-95 0.7746118065666359 1101 96-97 0.8256604804146583 1101 98-99 0.9461185742505336 1101 100-101 0.7287360371671596 1102 1 0.35934289030397437 1102 2 0.3533258152282315 1102 3 0.34309066661196397 1102 4 0.3322451653521412 1102 5 0.25582376923364336 1102 6 0.2369670065612013 1102 7 0.3167053539497502 1102 8 0.3036890300486803 1102 9 0.43012031932959616 1102 10-11 0.40541699979828394 1102 12-13 0.41202002593890086 1102 14-15 0.5301879627717909 1102 16-17 0.5336968004126845 1102 18-19 0.4464836593409416 1102 20-21 0.43894283441203896 1102 22-23 0.4260134554143278 1102 24-25 0.40175417020300586 1102 26-27 0.4353973337852537 1102 28-29 0.4144296146709081 1102 30-31 0.3884296726607701 1102 32-33 0.36738144265075334 1102 34-35 0.31546128044706023 1102 36-37 0.3690343472556208 1102 38-39 0.49641653068317027 1102 40-41 0.5048454937700484 1102 42-43 0.4812631988590539 1102 44-45 0.6347584091706651 1102 46-47 0.49862570393016625 1102 48-49 0.5583688958043993 1102 50-51 0.45899938563609766 1102 52-53 0.4939629570509396 1102 54-55 0.505105272513859 1102 56-57 0.5682651765635427 1102 58-59 0.5391214735800816 1102 60-61 0.568852654277805 1102 62-63 0.4529624298535353 1102 64-65 0.5131279917766349 1102 66-67 0.43272689466331826 1102 68-69 0.428506431495979 1102 70-71 0.15811551283606917 1102 72-73 0.1653521237723936 1102 74-75 0.03942466078699525 1102 76-77 0.08140518016322673 1102 78-79 0.29525796927877934 1102 80-81 0.34126897234963494 1102 82-83 0.1883962238936263 1102 84-85 0.2627122156670474 1102 86-87 0.022383836843530958 1102 88-89 0.2084662000848283 1102 90-91 0.0253477964687967 1102 92-93 0.005324996804578319 1102 94-95 0.14114110595113516 1102 96-97 0.15783644014443254 1102 98-99 -0.08249300771980828 1102 100-101 -0.35209948795613855 1103 1 0.3348012691822184 1103 2 0.2701159592034159 1103 3 0.3284219032814164 1103 4 0.2313798653523591 1103 5 0.2326345389266251 1103 6 0.21212750575470807 1103 7 0.3065938514897937 1103 8 0.2745036393639211 1103 9 0.35624274830617253 1103 10-11 0.33365654790755883 1103 12-13 0.31566532325392416 1103 14-15 0.5796261889600629 1103 16-17 0.6105965291674522 1103 18-19 0.5002969667642816 1103 20-21 0.49477482004222395 1103 22-23 0.41712862916681814 1103 24-25 0.3447180943736612 1103 26-27 0.516020771674782 1103 28-29 0.47912469283769354 1103 30-31 0.4443623365105722 1103 32-33 0.5336826846684062 1103 34-35 0.5768132024954795 1103 36-37 0.47523314252619286 1103 38-39 0.5406280124747909 1103 40-41 0.6936809717092629 1103 42-43 0.6257949332730988 1103 44-45 0.6594553624027029 1103 46-47 0.37371812547041117 1103 48-49 0.4366422163958106 1103 50-51 0.35207773140239595 1103 52-53 0.5586125435777447 1103 54-55 0.6373433972781442 1103 56-57 0.6361623548034103 1103 58-59 0.5086858618293846 1103 60-61 0.5109042952795164 1103 62-63 0.5950632913181764 1103 64-65 0.5658191365679031 1103 66-67 0.44934711084981416 1103 68-69 0.4020271008942764 1103 70-71 0.231733025093245 1103 72-73 0.2317336952901492 1103 74-75 0.41316650265506993 1103 76-77 0.26044163100561946 1103 78-79 0.44064584144194896 1103 80-81 0.42683896022563417 1103 82-83 0.5238222792037881 1103 84-85 0.4373324616841323 1103 86-87 0.49647597499322416 1103 88-89 0.548657158434942 1103 90-91 0.44185998332757137 1103 92-93 0.3811864081747167 1103 94-95 0.12830415352878788 1103 96-97 0.22135225141911619 1103 98-99 0.47914123949476206 1103 100-101 0.2583720057251071 1104 1 0.376816517062192 1104 2 0.39774764543955143 1104 3 0.37547017464476085 1104 4 0.3077028431736224 1104 5 0.278989724836606 1104 6 0.2671564522648211 1104 7 0.2301363335511155 1104 8 0.291465886591574 1104 9 0.4166247607001665 1104 10-11 0.44131106519392205 1104 12-13 0.42875036891602747 1104 14-15 0.5732793608452482 1104 16-17 0.6814818439097863 1104 18-19 0.5754498532613468 1104 20-21 0.5367666253259813 1104 22-23 0.6099286943983984 1104 24-25 0.6418978481244721 1104 26-27 0.6471454047471852 1104 28-29 0.6217973526550509 1104 30-31 0.6002056584283864 1104 32-33 0.6464028412241873 1104 34-35 0.6765981421562728 1104 36-37 0.8273817074316057 1104 38-39 0.7525225819464154 1104 40-41 0.8151543898436415 1104 42-43 0.8901051093983483 1104 44-45 0.8475801585540452 1104 46-47 0.7217723899844302 1104 48-49 0.8066458856717418 1104 50-51 0.9546788403391204 1104 52-53 1.030195020746703 1104 54-55 0.8884197182174844 1104 56-57 0.9053842644576804 1104 58-59 0.9339264032514407 1104 60-61 0.8391037000414201 1104 62-63 0.9729660962757762 1104 64-65 1.0189380211080206 1104 66-67 0.9011267096756512 1104 68-69 0.7966057457083657 1104 70-71 0.7757643362478426 1104 72-73 0.6147673710892398 1104 74-75 0.5326473001110408 1104 76-77 0.404673733093027 1104 78-79 0.3164704092827826 1104 80-81 0.5183741445048256 1104 82-83 0.5408426233003318 1104 84-85 0.5647123948217718 1104 86-87 0.7030011706879407 1104 88-89 0.5812270785262683 1104 90-91 0.5041061917424479 1104 92-93 0.4857414065439265 1104 94-95 0.5480754353291779 1104 96-97 0.394150685861387 1104 98-99 0.34857617100161065 1104 100-101 0.09175935895406795 1105 1 0.3109011349519015 1105 2 0.23684746089764985 1105 3 0.17109544602990923 1105 4 0.19509564603944796 1105 5 0.17400166578319443 1105 6 0.2818967366764582 1105 7 0.20334654848814893 1105 8 0.23730419531932512 1105 9 0.2882175201443715 1105 10-11 0.3187413192104245 1105 12-13 0.40406381876405817 1105 14-15 0.45841898850482465 1105 16-17 0.37590691446992963 1105 18-19 0.39750668131171096 1105 20-21 0.30686744490913753 1105 22-23 0.39239178601801683 1105 24-25 0.38371626432073924 1105 26-27 0.2791668170355095 1105 28-29 0.23723848921605395 1105 30-31 0.4078105487887811 1105 32-33 0.4827901621099784 1105 34-35 0.5089804966938303 1105 36-37 0.5345544169409422 1105 38-39 0.3719415463406861 1105 40-41 0.2777222361310976 1105 42-43 0.31374275594632905 1105 44-45 0.3474071505210432 1105 46-47 0.09552876753544837 1105 48-49 0.04708362991837589 1105 50-51 0.061577450847146054 1105 52-53 -0.013398254329288761 1105 54-55 -0.22026460657627212 1105 56-57 -0.21330399542073764 1105 58-59 -0.10288699231956144 1105 60-61 0.028280181804333893 1105 62-63 0.02323748142558202 1105 64-65 -0.08618301666879091 1105 66-67 -0.14477401763654996 1105 68-69 -0.207619042611217 1105 70-71 -0.24271156475085576 1105 72-73 -0.21701590073012866 1105 74-75 -0.27506207353203393 1105 76-77 -0.29206353894344517 1105 78-79 -0.26050730999642013 1105 80-81 -0.12225017703230279 1105 82-83 -0.16676226706006858 1105 84-85 -0.16781379384518758 1105 86-87 -0.008737593966856139 1105 88-89 0.008896829937469874 1105 90-91 -0.12101661003784159 1105 92-93 -0.03866629536662103 1105 94-95 0.0553689112301754 1105 96-97 -0.0575861790973633 1105 98-99 -0.14590114119986453 1105 100-101 -0.18766354052487344 1106 1 0.16556119539710323 1106 2 0.12280578955812871 1106 3 0.19446260605440813 1106 4 0.14261395165674884 1106 5 0.11045062045312903 1106 6 0.07013954119575772 1106 7 0.16889086682137133 1106 8 0.034986266597883287 1106 9 0.1082884708862295 1106 10-11 0.1500888561116156 1106 12-13 0.08629037995355304 1106 14-15 0.18224193180078174 1106 16-17 0.019282912184777956 1106 18-19 0.17712420677759155 1106 20-21 0.30684654885168783 1106 22-23 0.1755428539695103 1106 24-25 0.17419887697973024 1106 26-27 0.16153016113319296 1106 28-29 0.35291283278749574 1106 30-31 0.3806243946314751 1106 32-33 0.4106097160270821 1106 34-35 0.27373479068986484 1106 36-37 0.0022614772203155553 1106 38-39 0.2242773517351253 1106 40-41 0.3628424220101465 1106 42-43 0.5962506470836217 1106 44-45 0.4106029628744423 1106 46-47 0.3061518247053385 1106 48-49 0.06719953593979966 1106 50-51 0.2582542108846866 1106 52-53 0.21270398873787144 1106 54-55 0.05483409853913912 1106 56-57 0.3371935320388886 1106 58-59 0.4095945678682753 1106 60-61 -0.04704272458803871 1106 62-63 0.12221712118905259 1106 64-65 -0.2490905976242459 1106 66-67 0.048786162529641075 1106 68-69 -0.5864787505073359 1106 70-71 -0.48473708286284634 1106 72-73 -0.22389846776286149 1106 74-75 0.024294749653773096 1106 76-77 0.12804421953263656 1106 78-79 0.05478294870252398 1106 80-81 -0.6457622675103138 1106 82-83 -0.18580758562429978 1106 84-85 0.006205121879478526 1106 86-87 0.18805419024013403 1106 88-89 0.18450039530330997 1106 90-91 -0.15476374282078353 1106 92-93 -0.32309403574557294 1106 94-95 0.1002385936388599 1106 96-97 0.16698260008402954 1106 98-99 0.07198952641000034 1106 100-101 -0.22631607072111137 1107 1 0.14265841746628283 1107 2 0.1710770666423116 1107 3 0.1608100843191096 1107 4 0.1828788890495403 1107 5 0.12232523707810827 1107 6 0.1810848751314751 1107 7 0.13056493469206742 1107 8 0.12828918704320103 1107 9 0.17253826794998872 1107 10-11 0.24945745811049136 1107 12-13 0.2243462507795897 1107 14-15 0.3965445290173477 1107 16-17 0.2811754349485298 1107 18-19 0.2970797260923419 1107 20-21 0.30377807945006197 1107 22-23 0.343257075667708 1107 24-25 0.315384373558274 1107 26-27 0.36285025677951666 1107 28-29 0.3674738293605344 1107 30-31 0.26330621999244386 1107 32-33 0.2845773123437425 1107 34-35 0.308218432743395 1107 36-37 0.33726256077127914 1107 38-39 0.2966328446038915 1107 40-41 0.3639762938132094 1107 42-43 0.4302435691083559 1107 44-45 0.5223131185017493 1107 46-47 0.5348603237852387 1107 48-49 0.3815337214216825 1107 50-51 0.5361947661770117 1107 52-53 0.6023378036085916 1107 54-55 0.5480168182887937 1107 56-57 0.46338543809685007 1107 58-59 0.3333722672875652 1107 60-61 0.2644955321070199 1107 62-63 0.4714151098246262 1107 64-65 0.38553684449760084 1107 66-67 0.39298328799749527 1107 68-69 0.344857543307608 1107 70-71 0.281748410409957 1107 72-73 0.09159245428391571 1107 74-75 0.35167993916499896 1107 76-77 0.2808418011938123 1107 78-79 0.4605565306281605 1107 80-81 0.5887549829883021 1107 82-83 0.5880641439801515 1107 84-85 0.522489239960823 1107 86-87 0.4991541597537861 1107 88-89 0.5191872490753511 1107 90-91 0.4453986061707802 1107 92-93 0.3496679723865732 1107 94-95 0.08103761146418975 1107 96-97 0.15995173173730493 1107 98-99 -0.01742840470554441 1107 100-101 -0.34255510206328665 1108 1 0.15371612954648484 1108 2 0.15967029939849908 1108 3 0.1800051557926423 1108 4 0.08883108992687028 1108 5 0.12488938968788688 1108 6 0.10930533161128153 1108 7 0.06425473531064085 1108 8 0.05935575881612465 1108 9 0.15073268906213144 1108 10-11 0.15818763620355725 1108 12-13 0.12795831822478476 1108 14-15 0.20061296474135304 1108 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-0.4825711578431182 2316 74-75 -0.09790795224662574 2316 76-77 -0.06224707404400576 2316 78-79 -0.0951274557189663 2316 80-81 -0.289018850896273 2316 82-83 -0.3137641047820985 2316 84-85 -0.3230391987863328 2316 86-87 0.0050557608614170135 2316 88-89 0.016306285942729204 2316 90-91 -0.03942023013437179 2316 92-93 0.01502213625635207 2316 94-95 0.10269653499153719 2316 96-97 0.11110842250127462 2316 98-99 0.32583269096900835 2316 100-101 0.3407513472530539 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 10.0 4 43.0 5 65.0 6 163.0 7 404.0 8 901.0 9 1762.0 10 2932.0 11 4358.0 12 6250.0 13 8665.0 14 11387.0 15 14600.0 16 18080.0 17 21782.0 18 20732.0 19 18864.0 20 19299.0 21 19921.0 22 21777.0 23 25068.0 24 29040.0 25 34257.0 26 40737.0 27 49227.0 28 59127.0 29 70965.0 30 83862.0 31 99669.0 32 120073.0 33 147203.0 34 186552.0 35 242748.0 36 319561.0 37 362980.0 38 186795.0 39 9352.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.043466713881024 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22.52415108787787 26.834829797075532 28.561779202067978 22.079239912978625 20-21 22.8333292758768 26.041390482977494 28.940981712674535 22.18429852847117 22-23 22.58924006789969 25.975482634454888 28.84920204584336 22.586075251802065 24-25 22.582475659654783 26.161511181774884 28.8664808791738 22.389532279396533 26-27 22.151875340160558 26.589660624065008 28.559144611425147 22.699319424349287 28-29 22.32622835808013 26.996124760148987 28.35557926093798 22.3220676208329 30-31 22.402803628694038 26.463218418787054 28.374147657482794 22.759830295036107 32-33 22.084418702956558 26.4191101776502 28.394088212055955 23.102382907337283 34-35 22.870597036728803 26.52563756368997 28.233458315441855 22.37030708413937 36-37 22.97413039484954 26.07120172272227 28.233169485861236 22.72149839656695 38-39 22.40623070334442 26.52510806127124 28.18600924690202 22.882651988482316 40-41 22.968294309406975 26.269977210006353 28.246555993068938 22.515172487517738 42-43 22.671623576987106 26.33331311971523 28.015233690819286 22.979829612478383 44-45 22.572098083669808 26.58462190832741 28.26788431728911 22.575395690713673 46-47 23.051347481315414 26.819293427141726 27.53436481255657 22.594994278986285 48-49 22.714814659074058 26.825822243566904 27.508824967964536 22.950538129394502 50-51 22.890517281445103 27.059553871588136 27.490787729366172 22.559141117600582 52-53 23.287646372744515 26.590917641747243 27.514225073753494 22.607210911754745 54-55 22.696091341461525 26.897019539972955 27.70668130301897 22.70020781554655 56-57 22.784661929032872 26.99388947045766 27.492868097989785 22.72858050251968 58-59 22.676637681672617 26.629271671797504 27.472086543334743 23.222004103195136 60-61 22.342672122839126 26.87816343287381 27.762032387355784 23.01713205693128 62-63 22.531188930668396 26.935816201645263 27.794853522130474 22.738141345555867 64-65 22.22641492730882 26.973882521141192 27.57858371159894 23.221118839951043 66-67 22.0955040538453 26.98594599092693 27.5930379295799 23.325512025647868 68-69 22.806835442085468 26.523955098175435 27.65862892343757 23.01058053630153 70-71 22.51255193733393 26.699695867543255 27.238356896848593 23.549395298274224 72-73 22.47778542546858 26.834187981223568 27.349853820906823 23.338172772401034 74-75 22.421194972590037 27.181056251839685 27.179285725351505 23.21846305021877 76-77 22.53754069444475 26.732117129179827 27.42760206531915 23.302740111056274 78-79 22.576425882441466 26.99351323357892 27.343457793968255 23.086603090011355 80-81 22.7625026170919 26.930882658230594 27.11311635557393 23.193498369103576 82-83 22.675312494259586 27.05579825438324 27.152646632006928 23.116242619350245 84-85 22.54063656013361 27.165110523564355 27.132442806041844 23.161810110260188 86-87 22.7596157165963 27.199860479486677 26.741328437131223 23.299195366785796 88-89 22.61275170280611 27.25326352728296 26.610096794811643 23.523887975099285 90-91 22.42849959365212 27.21800430958928 26.81420163707734 23.53929445968126 92-93 22.569253711391635 26.905093987100702 26.951260914848795 23.574391386658863 94-95 22.337795874537754 26.69601611357227 27.156862332842824 23.809325679047156 96-97 22.531365983317215 26.357827829577975 27.211907675896274 23.89889851120854 98-99 22.294973776243122 26.657603030438505 26.7698324769134 24.277590716404976 100-101 22.45600794966393 26.43342931062338 27.023545789134722 24.087016950577965 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 132.0 1 106.0 2 112.5 3 144.0 4 124.5 5 132.5 6 233.0 7 550.0 8 894.5 9 1070.0 10 990.5 11 823.0 12 894.5 13 1043.5 14 1179.0 15 1335.5 16 1490.5 17 1679.5 18 1954.0 19 2312.5 20 2722.0 21 3219.0 22 4047.0 23 5151.0 24 6523.0 25 8363.5 26 11223.5 27 14698.0 28 17764.5 29 20924.5 30 24368.0 31 29571.5 32 37480.0 33 41146.0 34 41525.0 35 44134.0 36 48115.5 37 52661.5 38 56908.0 39 60451.0 40 64431.0 41 69724.0 42 72532.5 43 74455.5 44 77468.0 45 81604.0 46 84389.5 47 87136.0 48 88864.0 49 87745.5 50 86258.5 51 84136.5 52 82378.0 53 81613.5 54 81992.0 55 77126.5 56 69668.5 57 63215.0 58 57908.0 59 51222.5 60 41115.5 61 34199.5 62 29342.0 63 21880.5 64 14994.0 65 11217.0 66 8478.0 67 6550.5 68 5101.5 69 4038.5 70 3085.0 71 2001.5 72 1431.5 73 1159.5 74 815.0 75 570.0 76 431.0 77 297.0 78 200.0 79 138.0 80 75.5 81 40.5 82 21.5 83 12.5 84 6.5 85 4.5 86 3.5 87 1.0 88 0.5 89 1.0 90 0.5 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.639474330685658E-4 2 0.048955057398255584 3 8.410000818868501E-4 4 0.0 5 0.08728695586741413 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.3278948661371318E-4 26-27 0.007591132318083936 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.009959211496028488 36-37 0.0 38-39 0.0035410529763656847 40-41 0.004846816261400531 42-43 5.311579464548527E-4 44-45 4.4263162204571057E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 8.852632440914211E-5 68-69 8.631316629891355E-4 70-71 8.852632440914211E-5 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0012393685417279895 82-83 5.975526897617092E-4 84-85 0.0046033688692753896 86-87 0.002168894948023982 88-89 1.3278948661371318E-4 90-91 0.002965631867706261 92-93 9.737895685005632E-4 94-95 6.639474330685659E-5 96-97 0.0 98-99 1.7705264881828423E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2259215.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.76509873855973 #Duplication Level Percentage of deduplicated Percentage of total 1 74.93693003350931 41.03928372449679 2 14.121864447630278 15.467706016940566 3 4.803319206626704 7.891627518711955 4 2.1688684216000547 4.751131730394847 5 1.1466164532447443 3.139728163860279 6 0.6679925746747182 2.19496075852114 7 0.44052741722240124 1.6887869250859253 8 0.2970730575543888 1.301538826362555 9 0.2142654283435932 1.0560840605547006 >10 1.0902581497500858 10.637239681676823 >50 0.06707563952973328 2.5291434334461136 >100 0.03914794571910826 4.059822273202131 >500 0.002909387805564192 1.1170122204713564 >1k 0.0031518367893610057 3.1259346662749508 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4761 0.2107369152559628 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4251 0.18816270253163156 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3143 0.13911911880896682 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3026 0.13394032883103202 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2863 0.12672543339168693 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2854 0.12632706493184578 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2725 0.12061711700745613 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2685 0.11884659051927329 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2492 0.11030380021379108 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.2131581102285528E-5 0.0 0.0 0.0 4.4263162204571057E-5 14-15 4.4263162204571057E-5 0.0 0.0 0.0 4.4263162204571057E-5 16-17 4.4263162204571057E-5 0.0 0.0 0.0 4.4263162204571057E-5 18-19 4.4263162204571057E-5 0.0 0.0 0.0 4.4263162204571057E-5 20-21 4.4263162204571057E-5 0.0 0.0 2.2131581102285528E-5 4.4263162204571057E-5 22-23 4.4263162204571057E-5 0.0 0.0 1.1065790551142765E-4 4.4263162204571057E-5 24-25 1.3278948661371318E-4 0.0 0.0 2.4344739212514083E-4 6.639474330685658E-5 26-27 1.3278948661371318E-4 0.0 0.0 7.303421763754224E-4 8.852632440914211E-5 28-29 1.549210677159987E-4 0.0 0.0 0.0033418687464451145 8.852632440914211E-5 30-31 1.7705264881828423E-4 0.0 0.0 0.01237155383617761 8.852632440914211E-5 32-33 1.7705264881828423E-4 0.0 0.0 0.032555555801462015 8.852632440914211E-5 34-35 1.7705264881828423E-4 0.0 0.0 0.06143726913994463 8.852632440914211E-5 36-37 1.7705264881828423E-4 0.0 0.0 0.11152103717441678 8.852632440914211E-5 38-39 2.8771055432971185E-4 0.0 0.0 0.1863921760434487 8.852632440914211E-5 40-41 3.098421354319974E-4 0.0 0.0 0.26845607877072347 8.852632440914211E-5 42-43 3.098421354319974E-4 0.0 0.0 0.3466469548050982 8.852632440914211E-5 44-45 3.098421354319974E-4 0.0 0.0 0.45146212290552246 8.852632440914211E-5 46-47 3.098421354319974E-4 0.0 0.0 0.5754211086594238 8.852632440914211E-5 48-49 3.098421354319974E-4 0.0 0.0 0.7003981471440301 8.852632440914211E-5 50-51 3.098421354319974E-4 0.0 0.0 0.8405131871025997 8.852632440914211E-5 52-53 3.098421354319974E-4 0.0 0.0 0.9774412793824403 8.852632440914211E-5 54-55 3.098421354319974E-4 0.0 0.0 1.1252581095646055 8.852632440914211E-5 56-57 3.098421354319974E-4 0.0 0.0 1.2986369159199103 8.852632440914211E-5 58-59 3.098421354319974E-4 0.0 0.0 1.4878398027633493 8.852632440914211E-5 60-61 3.983684598411395E-4 0.0 0.0 1.6916495331342967 8.852632440914211E-5 62-63 3.983684598411395E-4 0.0 0.0 1.890723105149355 8.852632440914211E-5 64-65 3.983684598411395E-4 0.0 0.0 2.1031198889879894 8.852632440914211E-5 66-67 4.426316220457106E-4 0.0 0.0 2.354933018769794 8.852632440914211E-5 68-69 4.426316220457106E-4 0.0 0.0 2.6328392826711937 8.852632440914211E-5 70-71 4.868947842502816E-4 0.0 0.0 2.917606336714301 8.852632440914211E-5 72-73 5.090263653525672E-4 0.0 0.0 3.219304050300657 8.852632440914211E-5 74-75 5.311579464548527E-4 0.0 0.0 3.5228829482807082 8.852632440914211E-5 76-77 5.311579464548527E-4 0.0 0.0 3.855476349085855 8.852632440914211E-5 78-79 5.311579464548527E-4 0.0 0.0 4.231159938297152 8.852632440914211E-5 80-81 6.639474330685658E-4 0.0 0.0 4.623464344916265 8.852632440914211E-5 82-83 6.639474330685658E-4 0.0 0.0 5.032035463645558 8.852632440914211E-5 84-85 7.082105952731369E-4 0.0 0.0 5.446958346151208 8.852632440914211E-5 86-87 7.52473757477708E-4 0.0 0.0 5.888306336492985 8.852632440914211E-5 88-89 7.52473757477708E-4 0.0 0.0 6.35178590793705 8.852632440914211E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3320 0.0 57.434372 1 GTATCAA 5870 0.0 39.693882 1 ATCAACG 7475 0.0 30.585577 3 TATCAAC 7625 0.0 30.307657 2 TCAACGC 7580 0.0 30.161898 4 CAACGCA 7845 0.0 29.20363 5 AACGCAG 8195 0.0 28.051252 6 GTAAGGT 1425 0.0 25.016651 4 CGCAGAG 9170 0.0 24.913319 8 TGGTATC 1470 0.0 24.907454 2 TAAGGTG 1570 0.0 24.825443 5 ACGCAGA 9270 0.0 24.64457 7 TATACCG 140 1.7713166E-5 23.765818 5 GTGGTAT 1685 0.0 23.140862 1 AGGTAAG 1470 0.0 22.319666 2 TACATGG 6085 0.0 21.3333 2 AAGGTAA 1570 0.0 21.201374 1 GGTAAGG 1715 0.0 20.786432 3 GCAGAGT 10785 0.0 20.698246 9 CAGAGTA 9860 0.0 20.66504 10-11 >>END_MODULE