FastQCFastQC Report
Thu 26 May 2016
SRR938465_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938465_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271085
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50480.2222726141910144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41060.18079464220845984No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30860.135882188469388No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30240.13315221579113068No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27160.11959041603462663No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26430.11637609336506559No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.11540739338245817No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23460.10329864359986526No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG23080.10162543453899789No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32250.049.7963871
GTATCAA59300.042.5910951
ATCAACG74650.033.36253
TCAACGC75400.032.9045754
CAACGCA76050.032.4983445
TATCAAC76650.032.4919852
AACGCAG80600.030.835236
ACGCAGA90850.027.0881967
CGCAGAG90900.026.8642378
TGGTATC17000.025.7214832
GTGGTAT19200.024.3232981
TAAGGTG14850.023.6843995
GTAAGGT14150.023.1766034
TACATGG59800.022.8901392
GCAGAGT102850.022.864769
GTACATG64550.022.3688681
AGGTAAG14750.021.9115942
GAGTACT64550.021.45162412-13
ACATGGG62400.021.3270383
AGAGTAC89400.020.66995610-11
Produced by FastQC (version 0.11.5)