FastQCFastQC Report
Thu 26 May 2016
SRR938465_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938465_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2271085
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48180.21214529619102765No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41670.18348058306932588No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA30870.13592622028677923No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30830.13575009301721425No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29280.12892516132157097No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28790.12676760226939987No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27280.12011879784332158No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA27270.12007476602593034No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25370.11170872072159342No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31000.049.563681
GTATCAA54400.041.1855351
TATCAAC70900.031.198192
ATCAACG70950.031.1617743
TCAACGC71750.030.8136464
CAACGCA73150.030.3539095
AACGCAG76850.028.8613266
CGCAGAG85350.025.7087968
ACGCAGA85750.025.6996467
TGGTATC16050.025.4886072
TAAGGTG14150.025.2009495
GTGGTAT18950.022.843091
GTAAGGT14000.022.0748334
AGAGTAC87000.021.12686310-11
GCAGAGT101550.021.0930779
AGGTAAG13750.020.7573172
AAGGTAA14350.020.5523951
GGTAAGG15650.020.05173
TACATGG58450.019.8576562
GTACATG60500.019.8138031
Produced by FastQC (version 0.11.5)