##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938465_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2271085 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77189493127734 31.0 31.0 34.0 30.0 34.0 2 32.006888337512684 31.0 31.0 34.0 30.0 34.0 3 32.078743860313466 33.0 31.0 34.0 30.0 34.0 4 35.75511132344232 37.0 35.0 37.0 35.0 37.0 5 35.619884328415715 37.0 35.0 37.0 33.0 37.0 6 35.65633254589766 37.0 35.0 37.0 33.0 37.0 7 35.58321330993776 37.0 35.0 37.0 33.0 37.0 8 35.56373143233301 37.0 35.0 37.0 33.0 37.0 9 37.132886703932265 39.0 37.0 39.0 33.0 39.0 10-11 37.060924183815224 39.0 37.0 39.0 33.0 39.0 12-13 36.960553435912786 39.0 37.0 39.0 33.0 39.0 14-15 38.01612533216502 40.0 37.0 41.0 33.0 41.0 16-17 37.93371736416735 40.0 37.0 41.0 33.0 41.0 18-19 37.92437865601684 40.0 37.0 41.0 33.0 41.0 20-21 37.90296290099226 40.0 37.0 41.0 32.5 41.0 22-23 37.94000973103164 40.0 37.0 41.0 33.0 41.0 24-25 37.86947318132083 40.0 37.0 41.0 32.0 41.0 26-27 37.69857094736657 40.0 37.0 41.0 32.0 41.0 28-29 37.56801616848335 39.5 37.0 41.0 32.0 41.0 30-31 37.3223166460084 39.0 36.0 41.0 31.0 41.0 32-33 37.10255758811317 39.0 36.0 41.0 31.0 41.0 34-35 36.939862004284294 39.0 36.0 40.5 30.5 41.0 36-37 36.95221909351697 39.0 36.0 41.0 30.5 41.0 38-39 36.80184163076239 39.0 35.0 41.0 30.0 41.0 40-41 36.62267308357019 39.0 35.0 40.5 30.0 41.0 42-43 36.40030117763095 39.0 35.0 40.0 30.0 41.0 44-45 36.13691539506448 38.5 35.0 40.0 30.0 41.0 46-47 35.807146143803514 38.0 35.0 40.0 28.5 41.0 48-49 35.42787962581762 38.0 34.0 40.0 27.5 41.0 50-51 35.060911194429096 38.0 34.0 40.0 26.5 41.0 52-53 34.539855619670774 38.0 33.0 40.0 24.5 41.0 54-55 34.213990669657896 37.5 33.0 40.0 24.0 41.0 56-57 33.85260789446454 37.0 33.0 40.0 23.0 41.0 58-59 33.3893581702138 36.5 32.0 39.0 22.5 41.0 60-61 32.97853184711272 36.0 31.0 39.0 22.0 40.0 62-63 32.49589139111922 35.5 31.0 39.0 20.0 40.0 64-65 31.997047006166657 35.0 31.0 38.5 18.5 40.0 66-67 31.506973318920252 35.0 30.0 38.0 17.0 40.0 68-69 30.94013434107486 34.5 29.0 37.5 13.0 40.0 70-71 30.37121728160769 34.0 29.0 37.0 8.0 39.0 72-73 29.782447376474238 34.0 28.0 36.0 7.0 39.0 74-75 29.163265355545917 33.5 27.5 36.0 6.0 38.5 76-77 27.9052677464736 31.5 26.0 34.5 2.0 36.5 78-79 28.525234634546926 33.0 27.0 35.0 2.0 37.0 80-81 28.225367830794532 33.0 26.5 35.0 2.0 37.0 82-83 27.721223115823495 33.0 26.0 35.0 2.0 36.0 84-85 27.13292104874983 32.5 25.5 35.0 2.0 36.0 86-87 26.49287961480966 32.0 24.5 35.0 2.0 35.0 88-89 25.737095485197603 31.5 22.5 34.0 2.0 35.0 90-91 25.194206513626746 31.0 20.0 34.0 2.0 35.0 92-93 24.41679285451667 31.0 17.5 34.0 2.0 35.0 94-95 23.620759020468192 31.0 4.5 34.0 2.0 35.0 96-97 22.646058161627593 30.0 2.0 34.0 2.0 35.0 98-99 21.46198270870531 29.5 2.0 34.0 2.0 35.0 100-101 18.32270104377423 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34546801999149324 1101 2 0.3645156769806803 1101 3 0.3005317450158884 1101 4 0.3190282853815205 1101 5 0.23407481324210977 1101 6 0.20345772206624702 1101 7 0.2984234220287334 1101 8 0.3662636126165211 1101 9 0.4082271706685532 1101 10-11 0.4165457741471954 1101 12-13 0.3311766581888378 1101 14-15 0.563464646285027 1101 16-17 0.6032732444851447 1101 18-19 0.6526160558927998 1101 20-21 0.6776574292464019 1101 22-23 0.5734400369799033 1101 24-25 0.6136680393462797 1101 26-27 0.5708602647723069 1101 28-29 0.582412202883809 1101 30-31 0.6488959914477661 1101 32-33 0.5797410374287182 1101 34-35 0.4552489957866612 1101 36-37 0.6356539644155106 1101 38-39 0.7006023895448692 1101 40-41 0.9141665465400877 1101 42-43 0.7369316216133441 1101 44-45 0.7833581556177762 1101 46-47 0.9690589705288986 1101 48-49 0.9694173933061236 1101 50-51 1.0103200854732108 1101 52-53 0.998618309076825 1101 54-55 1.1484726917180268 1101 56-57 1.1284252038405 1101 58-59 1.1789619712823836 1101 60-61 1.2183300868713758 1101 62-63 1.3160338848046962 1101 64-65 1.2740408360798696 1101 66-67 1.5908431383262958 1101 68-69 1.6390984927232175 1101 70-71 1.6768090193569094 1101 72-73 1.6637438082281868 1101 74-75 1.7330545925064271 1101 76-77 1.5974748256344569 1101 78-79 1.9044139327420737 1101 80-81 2.2025420779700227 1101 82-83 2.3448157925515254 1101 84-85 2.594804601511644 1101 86-87 2.6756023496114736 1101 88-89 2.8300200017205555 1101 90-91 3.1828719617726513 1101 92-93 3.323121664238169 1101 94-95 3.165120319424094 1101 96-97 3.703662001392459 1101 98-99 4.048266809626327 1101 100-101 4.420966659195411 1102 1 0.2590957399134055 1102 2 0.25539205346868954 1102 3 0.23920976466670396 1102 4 0.21784891482370483 1102 5 0.18199878534559844 1102 6 0.1764432610379032 1102 7 0.22012025320329798 1102 8 0.22545840305922837 1102 9 0.23305971568537132 1102 10-11 0.27463305078765643 1102 12-13 0.3417776565953474 1102 14-15 0.5255217499864031 1102 16-17 0.4779511537639749 1102 18-19 0.48702566929195257 1102 20-21 0.49962133761006555 1102 22-23 0.37523243189083644 1102 24-25 0.3733866367943932 1102 26-27 0.4323824092998194 1102 28-29 0.3719863050192842 1102 30-31 0.328159396469232 1102 32-33 0.12508954844177822 1102 34-35 0.05056186584894817 1102 36-37 0.5096144558467373 1102 38-39 0.5463916986392903 1102 40-41 0.44507633354516685 1102 42-43 0.43956560284550505 1102 44-45 0.47931635683048057 1102 46-47 0.5460743513237105 1102 48-49 0.6500842469919732 1102 50-51 0.6609311553225794 1102 52-53 0.6802339018778127 1102 54-55 0.6380134523189085 1102 56-57 0.7098663859243786 1102 58-59 0.784953215105574 1102 60-61 0.8414356553675404 1102 62-63 1.031998961271782 1102 64-65 1.0176985863770724 1102 66-67 0.956767515127023 1102 68-69 1.2424587445552753 1102 70-71 1.3194827345006352 1102 72-73 1.4177393887502916 1102 74-75 1.468442607489422 1102 76-77 1.5239253548450868 1102 78-79 1.863465009673643 1102 80-81 1.9271467508435798 1102 82-83 1.8807041604949042 1102 84-85 1.9436168380847754 1102 86-87 2.1672017775167305 1102 88-89 2.2481922877771296 1102 90-91 2.4920286968776075 1102 92-93 2.7204030644906467 1102 94-95 3.0225950315946726 1102 96-97 3.308825915274724 1102 98-99 3.439428543662057 1102 100-101 3.9019349663422283 1103 1 0.24731684337854176 1103 2 0.30777106178222624 1103 3 0.29522791123649483 1103 4 0.22876523800380966 1103 5 0.0953681265500137 1103 6 0.2013814771200586 1103 7 0.21008091234818949 1103 8 0.17524685317799538 1103 9 0.3134281486880255 1103 10-11 0.35853659364779134 1103 12-13 0.31511167399089857 1103 14-15 0.5391471778891486 1103 16-17 0.45345906996490015 1103 18-19 0.419590815302854 1103 20-21 0.5250278452249901 1103 22-23 0.40594886335653513 1103 24-25 0.43953461691874907 1103 26-27 0.3810560199257722 1103 28-29 0.39115187282003205 1103 30-31 0.5337286817843037 1103 32-33 0.4578336058331445 1103 34-35 0.5181342600085159 1103 36-37 0.5585606361597186 1103 38-39 0.7073898909684786 1103 40-41 0.6655647976362715 1103 42-43 0.7468639645892594 1103 44-45 0.7187621136070703 1103 46-47 0.6985191824331167 1103 48-49 0.7050462815385785 1103 50-51 0.8189785652010144 1103 52-53 0.9148854012839891 1103 54-55 0.9366828057491148 1103 56-57 0.8774613759690837 1103 58-59 1.1371158705478024 1103 60-61 1.1381381843601304 1103 62-63 1.2017476557317508 1103 64-65 1.097322228440781 1103 66-67 1.229097161801608 1103 68-69 1.349812886479267 1103 70-71 1.2486074113903953 1103 72-73 1.320620418686019 1103 74-75 1.523968229258319 1103 76-77 1.345343415834332 1103 78-79 1.4279312883169268 1103 80-81 1.6564649664017743 1103 82-83 1.8980945868257706 1103 84-85 2.0610679567448322 1103 86-87 2.1268519670165205 1103 88-89 2.381040373567263 1103 90-91 2.6113309315779 1103 92-93 2.8207945081821357 1103 94-95 2.92237627722832 1103 96-97 3.287451408672794 1103 98-99 3.474725608598483 1103 100-101 3.823636949540493 1104 1 0.28823704806677597 1104 2 0.3135838927619261 1104 3 0.25404121921634726 1104 4 0.20195182618718377 1104 5 0.15489985517261573 1104 6 0.21551909653233992 1104 7 0.2990386021098814 1104 8 0.31125882361047275 1104 9 0.3131596332942266 1104 10-11 0.4211369827111824 1104 12-13 0.518545345010061 1104 14-15 0.5897907224870451 1104 16-17 0.6530039323162811 1104 18-19 0.5662099550152249 1104 20-21 0.5967603941580677 1104 22-23 0.509321611396615 1104 24-25 0.5715519138529643 1104 26-27 0.5576699921364607 1104 28-29 0.5007444838604798 1104 30-31 0.5069525863064044 1104 32-33 0.4481550860911767 1104 34-35 0.5225789604394393 1104 36-37 0.6056210010161678 1104 38-39 0.5443154627196876 1104 40-41 0.6514028414813495 1104 42-43 0.7195102770860089 1104 44-45 0.7012061958847227 1104 46-47 0.6997260820618934 1104 48-49 0.7789965161447725 1104 50-51 0.8209121194038076 1104 52-53 0.9329617196864604 1104 54-55 1.001955166348253 1104 56-57 1.1086087154763788 1104 58-59 1.4768981702726336 1104 60-61 1.3754571189948805 1104 62-63 1.4805313296713223 1104 64-65 1.5001583918668828 1104 66-67 1.548530496973715 1104 68-69 1.5681801912012467 1104 70-71 1.6702362046773445 1104 72-73 1.8856715177333179 1104 74-75 1.777970000973184 1104 76-77 1.7903955126880753 1104 78-79 2.0615982107898034 1104 80-81 2.22220925700195 1104 82-83 2.284866066434823 1104 84-85 2.6060290747423416 1104 86-87 2.6495806593871123 1104 88-89 2.4847943972420445 1104 90-91 2.858613531455891 1104 92-93 3.1657447152066958 1104 94-95 3.3214542391721444 1104 96-97 3.7563585834690123 1104 98-99 4.140840304421648 1104 100-101 4.4835603780359 1105 1 0.20605838670213572 1105 2 0.2254715207455078 1105 3 0.16519797539606884 1105 4 0.14017619277449 1105 5 0.008307635506938027 1105 6 0.09537227403277626 1105 7 0.14275842314732756 1105 8 0.09871810047820873 1105 9 0.14528043874011587 1105 10-11 0.23349937236078944 1105 12-13 0.3615854781453933 1105 14-15 0.3992282905177902 1105 16-17 0.3669001233570981 1105 18-19 0.4391046953689184 1105 20-21 0.29729272579925237 1105 22-23 0.3050452297648647 1105 24-25 0.2369189033817065 1105 26-27 0.3863226831515405 1105 28-29 0.4104214819865959 1105 30-31 0.5339601485610856 1105 32-33 0.47017350467064745 1105 34-35 0.5256622901190511 1105 36-37 0.5301051111311281 1105 38-39 0.5043099535399378 1105 40-41 0.528700636475044 1105 42-43 0.6046071833670439 1105 44-45 0.5151520457948493 1105 46-47 0.5247905623403639 1105 48-49 0.6174888118714392 1105 50-51 0.6043062316347587 1105 52-53 0.7744105767699452 1105 54-55 0.7133211379178377 1105 56-57 0.6884184295518594 1105 58-59 0.7860315896432155 1105 60-61 0.9785523629905484 1105 62-63 1.2073061338476236 1105 64-65 1.3008447517859736 1105 66-67 1.436813481995987 1105 68-69 1.4251621710365008 1105 70-71 1.6089319016076473 1105 72-73 1.559302467504466 1105 74-75 1.8511578335017447 1105 76-77 1.634567505848306 1105 78-79 1.8939673105822052 1105 80-81 2.276434931304582 1105 82-83 2.0943002984570747 1105 84-85 2.2715027212283765 1105 86-87 2.3541135324148215 1105 88-89 2.4902283738213598 1105 90-91 2.792468870689831 1105 92-93 3.019085108169431 1105 94-95 3.0179927014437915 1105 96-97 3.4669021812874554 1105 98-99 3.7984851968020124 1105 100-101 4.111492586530712 1106 1 0.14176187597273326 1106 2 0.10264180785552668 1106 3 0.07522515227950777 1106 4 0.09290104284931289 1106 5 -0.04159002713031157 1106 6 0.07158778935699672 1106 7 0.033229363482568886 1106 8 0.0691850076282563 1106 9 0.09893047134483623 1106 10-11 0.12186259649657671 1106 12-13 0.049015886854057555 1106 14-15 0.008959857542166105 1106 16-17 -0.04129289659397983 1106 18-19 0.13574226279284574 1106 20-21 0.2545233410673049 1106 22-23 0.1667601250339743 1106 24-25 0.056383262463349126 1106 26-27 0.021680618914587058 1106 28-29 0.2949528974838316 1106 30-31 0.3451972810040971 1106 32-33 0.21774219132231565 1106 34-35 0.35409662270227926 1106 36-37 0.4525838049822184 1106 38-39 0.4014945452647538 1106 40-41 0.3416480090621974 1106 42-43 0.549054768921458 1106 44-45 0.02216196667656334 1106 46-47 -0.12869993328909146 1106 48-49 0.2491041779301142 1106 50-51 0.20963324002906347 1106 52-53 0.337080809224652 1106 54-55 0.5561865463814897 1106 56-57 0.3992447048110961 1106 58-59 0.8837457725085827 1106 60-61 0.6058263067294902 1106 62-63 0.8491878409026867 1106 64-65 0.5201063208080967 1106 66-67 0.5442899519412272 1106 68-69 0.462985061435635 1106 70-71 0.5715842297355351 1106 72-73 0.7358767289981039 1106 74-75 1.087740236752417 1106 76-77 1.2804782518367936 1106 78-79 1.4786058693404378 1106 80-81 1.3725221762621587 1106 82-83 1.7275312944276564 1106 84-85 1.631934253197965 1106 86-87 1.9147294789506155 1106 88-89 2.1801016865741403 1106 90-91 2.3142333703675533 1106 92-93 2.0369387374707095 1106 94-95 2.5375354626302276 1106 96-97 2.8082971733530115 1106 98-99 3.1397597587114063 1106 100-101 3.576855064338769 1107 1 0.26914406765091314 1107 2 0.22547440171553035 1107 3 0.19682868545614696 1107 4 0.159600773030661 1107 5 -0.0010495750658563452 1107 6 0.06583422857286791 1107 7 0.16237548821313652 1107 8 0.24423067456190495 1107 9 0.2058431101915943 1107 10-11 0.291425776180958 1107 12-13 0.37420664769317824 1107 14-15 0.48362216593564256 1107 16-17 0.4577275649566914 1107 18-19 0.391480910440535 1107 20-21 0.3272382484567231 1107 22-23 0.30875609921460523 1107 24-25 0.3269956722297849 1107 26-27 0.36728046163555916 1107 28-29 0.4552578383877943 1107 30-31 0.4564212712210818 1107 32-33 0.4920412375362986 1107 34-35 0.6531464740437727 1107 36-37 0.5933202556702994 1107 38-39 0.6815367858143659 1107 40-41 0.7413774447563881 1107 42-43 0.7625334071265897 1107 44-45 0.6508225960691192 1107 46-47 0.637703880254513 1107 48-49 0.6307254286403108 1107 50-51 0.779680060002768 1107 52-53 0.8786609978867403 1107 54-55 0.857426628236432 1107 56-57 1.0032332777112316 1107 58-59 0.909503032478014 1107 60-61 0.8970818618728131 1107 62-63 0.9274848080072076 1107 64-65 1.170627607673545 1107 66-67 1.4230988942943164 1107 68-69 1.4299292761207845 1107 70-71 1.2392846313717598 1107 72-73 1.5099886335365689 1107 74-75 1.4563876043655952 1107 76-77 1.5661893029073752 1107 78-79 1.6487330201966337 1107 80-81 1.769348915443043 1107 82-83 2.0213315398760585 1107 84-85 2.262157124565981 1107 86-87 2.563734081653749 1107 88-89 2.657754169756849 1107 90-91 2.809255292678113 1107 92-93 2.9191644248753654 1107 94-95 3.077111016580499 1107 96-97 3.399082346349033 1107 98-99 3.4175038303759386 1107 100-101 3.670578670815292 1108 1 0.12777200509439268 1108 2 0.06262467473903399 1108 3 0.10440870564728044 1108 4 0.06439585653028956 1108 5 0.036059083165213224 1108 6 0.025312430315288736 1108 7 0.15539341234818949 1108 8 0.07882347277932666 1108 9 0.05460034431046523 1108 10-11 0.07867214059913152 1108 12-13 0.13746088984003535 1108 14-15 0.22711284380667962 1108 16-17 0.3073033329607924 1108 18-19 0.2980535376010778 1108 20-21 0.3229960150510607 1108 22-23 0.2890712983770527 1108 24-25 0.17318668966645134 1108 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2316 72-73 0.8188508040092941 2316 74-75 0.8859589750609764 2316 76-77 0.8576641859949348 2316 78-79 0.9956391470955097 2316 80-81 1.279252876872654 2316 82-83 1.3869257508744113 2316 84-85 1.7520522326577996 2316 86-87 1.8410347571555015 2316 88-89 2.0662192313557313 2316 90-91 2.1164774778822952 2316 92-93 1.9461261270440566 2316 94-95 2.566617004808073 2316 96-97 2.891302876072018 2316 98-99 2.9226552780654025 2316 100-101 3.372375715880363 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 22.0 4 30.0 5 71.0 6 179.0 7 398.0 8 952.0 9 1830.0 10 2951.0 11 4553.0 12 6500.0 13 9015.0 14 11684.0 15 15230.0 16 18810.0 17 22948.0 18 22693.0 19 20481.0 20 21413.0 21 22927.0 22 25003.0 23 28189.0 24 32807.0 25 38172.0 26 44823.0 27 53753.0 28 63708.0 29 75697.0 30 88958.0 31 105245.0 32 125422.0 33 151875.0 34 187929.0 35 236904.0 36 299316.0 37 332735.0 38 185978.0 39 11880.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.05817224645686 19.115065367492072 14.990073018027083 23.83668936802398 2 16.015048027259134 20.192153088127434 39.148306326030315 24.644492558583124 3 19.402214187869124 23.251174220558404 30.13481245274827 27.2117991388242 4 13.489983862338924 16.75516328098684 36.203840895431036 33.55101196124319 5 13.912405689755717 37.01519234066599 34.555026669352486 14.51737530022581 6 33.331645447 36.059020248031224 16.60012725195226 14.009207053016508 7 29.15751722194458 32.214073889792765 20.178813210425854 18.449595677836804 8 26.589229377147927 35.54411217545799 19.242960963592292 18.623697483801795 9 26.103910685861603 16.51395698531759 19.71330883696559 37.668823491855214 10-11 25.239786269558383 26.352051992769976 27.287618032790494 21.120543704881147 12-13 25.99195979014436 23.37321148261734 27.510661203785858 23.124167523452446 14-15 22.426791599609878 25.10564333787595 25.605184306179645 26.862380756334524 16-17 22.487379380340233 28.204118295880605 26.630200983230484 22.678301340548682 18-19 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26.312518191653833 28.022985003251932 22.989252558060272 44-45 22.60323479557354 26.597137107063357 28.252095034641854 22.547533062721254 46-47 23.03258134327865 26.80456698009982 27.556190102968404 22.606661573653124 48-49 22.686975608574755 26.85934256093453 27.55590389615536 22.897777934335352 50-51 22.920080049844017 26.9706770112083 27.527019023946703 22.58222391500098 52-53 23.311214683730462 26.658667553173927 27.498200199464133 22.531917563631477 54-55 22.67467370764936 26.924141307820122 27.724045494564383 22.677139489966137 56-57 22.769909536631168 26.99852713570826 27.488535215546754 22.743028112113812 58-59 22.726538196500794 26.68713412311736 27.439483770972906 23.14684390940894 60-61 22.338816028462166 26.95561812966049 27.72670331581601 22.978862526061334 62-63 22.525356822840184 26.97631308383438 27.77619507856377 22.722135014761665 64-65 22.234042318979693 26.96220088636048 27.59936329992052 23.204393494739296 66-67 22.14505637730025 26.9938894778151 27.580327883289137 23.280726261595515 68-69 22.780820503359305 26.568113033371986 27.639959200055124 23.011107263213585 70-71 22.48387885085763 26.684976564065195 27.265425996825304 23.565718588251872 72-73 22.51324807305759 26.85069030881715 27.30327134387308 23.332790274252176 74-75 22.476767712348945 27.166882789503692 27.160101889625444 23.196247608521915 76-77 22.518179636605414 26.798006239308524 27.39628855811209 23.287525565973972 78-79 22.606991812283557 27.004515462873474 27.326520143455664 23.06197258138731 80-81 22.797297327048526 26.953592666060494 27.073579368451643 23.175530638439337 82-83 22.68336162034957 27.058356529126964 27.19954576387163 23.058736086651837 84-85 22.574654468788143 27.17373786655723 27.09958538015919 23.152022284495438 86-87 22.80593759157361 27.152352727619384 26.777108705621682 23.264600975185328 88-89 22.60323206820354 27.30138398637303 26.569707115697366 23.525676829726063 90-91 22.471326809969323 27.140870978075288 26.88127939834461 23.50652281361078 92-93 22.596148659701257 26.874312413986388 26.994321422918045 23.535217503394314 94-95 22.372456336949078 26.73631766314339 27.15543451698197 23.73579148292556 96-97 22.58444752177924 26.397801051039483 27.14180666949938 23.875944757681903 98-99 22.31787889929263 26.704284515991255 26.76804258757378 24.209793997142334 100-101 22.442257957205644 26.463489354469623 27.0413225833368 24.052930104987936 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 149.0 1 119.0 2 108.5 3 143.0 4 146.5 5 147.5 6 250.0 7 573.0 8 937.0 9 1090.0 10 1010.0 11 878.5 12 916.0 13 1050.0 14 1218.5 15 1358.5 16 1483.0 17 1719.5 18 2019.0 19 2356.0 20 2801.5 21 3314.0 22 4037.0 23 5137.5 24 6625.0 25 8616.0 26 11444.0 27 15046.5 28 18043.0 29 20998.5 30 24428.5 31 29645.5 32 37964.5 33 41604.5 34 41761.5 35 44516.5 36 48499.5 37 53170.0 38 57279.0 39 60705.5 40 64812.5 41 69465.5 42 72039.5 43 74376.0 44 77637.0 45 81890.0 46 84524.0 47 87441.0 48 89355.5 49 87938.0 50 86640.5 51 84613.0 52 82343.5 53 81823.0 54 82318.5 55 77225.5 56 69817.0 57 63591.0 58 58332.0 59 51455.0 60 41638.5 61 34632.0 62 29266.5 63 22039.5 64 15503.5 65 11510.0 66 8631.0 67 6746.0 68 5260.0 69 4077.0 70 3151.0 71 2169.0 72 1575.0 73 1233.0 74 902.0 75 635.5 76 431.5 77 309.5 78 205.0 79 122.0 80 80.0 81 44.5 82 20.0 83 7.5 84 7.0 85 5.5 86 4.0 87 3.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 8.806363478249384E-5 2 0.04491245373907185 3 1.7612726956498767E-4 4 0.0 5 0.10461959812160265 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0013209545217374075 26-27 0.011954638421723538 28-29 0.0 30-31 0.0 32-33 3.962863565212222E-4 34-35 0.015102913365197692 36-37 0.0031923067608654013 38-39 0.011800527060854174 40-41 0.007243233960860117 42-43 0.005548008991297111 44-45 0.0026639249521704387 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 2.201590869562346E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1007954347811729E-4 68-69 1.541113608693642E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 8.586204391293149E-4 84-85 0.0039188317478209755 86-87 0.0012989386130417841 88-89 2.201590869562346E-5 90-91 7.70556804346821E-4 92-93 2.4217499565185803E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 6.384613521730802E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2271085.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.357783085395106 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48523865699273 40.48852445980698 2 14.370100639330838 15.622536269360864 3 4.906976558717312 8.001971021516226 4 2.191439720543026 4.764872198959868 5 1.1662877608249056 3.169840855903569 6 0.6837370426943888 2.2299857912538674 7 0.433638661267958 1.650014540065135 8 0.31083841579493293 1.3517189744311053 9 0.23067272580613454 1.1284972193778016 >10 1.1042986799213128 10.613944586649549 >50 0.07106669668521523 2.6396695257523075 >100 0.039539478730456074 4.0742790405840905 >500 0.003001363415237054 1.132155194895066 >1k 0.0031635992755197537 3.131990321443734 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4818 0.21214529619102765 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4167 0.18348058306932588 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3087 0.13592622028677923 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3083 0.13575009301721425 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2928 0.12892516132157097 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2879 0.12676760226939987 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2728 0.12011879784332158 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2727 0.12007476602593034 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2537 0.11170872072159342 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.403181739124692E-5 2 0.0 0.0 0.0 0.0 4.403181739124692E-5 3 0.0 0.0 0.0 0.0 4.403181739124692E-5 4 0.0 0.0 0.0 0.0 4.403181739124692E-5 5 0.0 0.0 0.0 0.0 4.403181739124692E-5 6 0.0 0.0 0.0 0.0 4.403181739124692E-5 7 0.0 0.0 0.0 0.0 4.403181739124692E-5 8 0.0 0.0 0.0 0.0 4.403181739124692E-5 9 0.0 0.0 0.0 0.0 4.403181739124692E-5 10-11 8.806363478249384E-5 0.0 0.0 0.0 4.403181739124692E-5 12-13 8.806363478249384E-5 0.0 0.0 0.0 4.403181739124692E-5 14-15 1.7612726956498767E-4 0.0 0.0 0.0 4.403181739124692E-5 16-17 1.7612726956498767E-4 0.0 0.0 0.0 4.403181739124692E-5 18-19 2.4217499565185803E-4 0.0 0.0 0.0 4.403181739124692E-5 20-21 2.641909043474815E-4 0.0 0.0 0.0 4.403181739124692E-5 22-23 2.641909043474815E-4 0.0 0.0 6.604772608687038E-5 4.403181739124692E-5 24-25 2.641909043474815E-4 0.0 0.0 1.9814317826061112E-4 4.403181739124692E-5 26-27 2.8620681304310496E-4 0.0 0.0 5.28381808694963E-4 4.403181739124692E-5 28-29 3.3023863043435186E-4 0.0 0.0 0.0027299726782573086 4.403181739124692E-5 30-31 3.5225453912997534E-4 0.0 0.0 0.01133819297824608 4.403181739124692E-5 32-33 3.5225453912997534E-4 0.0 0.0 0.02998566764343915 4.403181739124692E-5 34-35 4.4031817391246915E-4 0.0 0.0 0.05748353760427284 4.403181739124692E-5 36-37 5.28381808694963E-4 0.0 0.0 0.10545620265203637 4.403181739124692E-5 38-39 5.28381808694963E-4 0.0 0.0 0.1782187808910719 4.403181739124692E-5 40-41 5.28381808694963E-4 0.0 0.0 0.2542837454344509 4.403181739124692E-5 42-43 6.604772608687037E-4 0.0 0.0 0.3332768258343479 4.403181739124692E-5 44-45 7.485408956511975E-4 0.0 0.0 0.4364213580733438 4.403181739124692E-5 46-47 7.485408956511975E-4 0.0 0.0 0.5571566013601429 4.403181739124692E-5 48-49 7.485408956511975E-4 0.0 0.0 0.6845846808904114 4.403181739124692E-5 50-51 7.485408956511975E-4 0.0 0.0 0.8262350374380527 4.403181739124692E-5 52-53 7.705568043468211E-4 0.0 0.0 0.9594753168639659 4.403181739124692E-5 54-55 7.925727130424445E-4 0.0 0.0 1.1047362824376896 8.806363478249384E-5 56-57 7.925727130424445E-4 0.0 0.0 1.2757558611852926 8.806363478249384E-5 58-59 7.925727130424445E-4 0.0 0.0 1.467338298654608 8.806363478249384E-5 60-61 7.925727130424445E-4 0.0 0.0 1.6704790881891256 8.806363478249384E-5 62-63 7.925727130424445E-4 0.0 0.0 1.8704936186888645 8.806363478249384E-5 64-65 7.925727130424445E-4 0.0 0.0 2.0838057580407603 8.806363478249384E-5 66-67 7.925727130424445E-4 0.0 0.0 2.3369006444056475 8.806363478249384E-5 68-69 7.925727130424445E-4 0.0 0.0 2.609809848596596 8.806363478249384E-5 70-71 7.925727130424445E-4 0.0 0.0 2.899208968400566 8.806363478249384E-5 72-73 7.925727130424445E-4 0.0 0.0 3.200056360726261 8.806363478249384E-5 74-75 7.925727130424445E-4 2.201590869562346E-5 0.0 3.5039859802693427 8.806363478249384E-5 76-77 7.925727130424445E-4 4.403181739124692E-5 0.0 3.836536281116735 8.806363478249384E-5 78-79 7.925727130424445E-4 4.403181739124692E-5 0.0 4.202066413190171 8.806363478249384E-5 80-81 8.806363478249383E-4 4.403181739124692E-5 0.0 4.5846368585940205 8.806363478249384E-5 82-83 8.806363478249383E-4 4.403181739124692E-5 0.0 4.988760878610885 8.806363478249384E-5 84-85 9.246681652161853E-4 4.403181739124692E-5 0.0 5.402395771184258 8.806363478249384E-5 86-87 9.246681652161853E-4 4.403181739124692E-5 0.0 5.83842084290108 8.806363478249384E-5 88-89 9.246681652161853E-4 4.403181739124692E-5 0.0 6.304233439083081 8.806363478249384E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3100 0.0 49.56368 1 GTATCAA 5440 0.0 41.185535 1 TATCAAC 7090 0.0 31.19819 2 ATCAACG 7095 0.0 31.161774 3 TCAACGC 7175 0.0 30.813646 4 CAACGCA 7315 0.0 30.353909 5 AACGCAG 7685 0.0 28.861326 6 CGCAGAG 8535 0.0 25.708796 8 ACGCAGA 8575 0.0 25.699646 7 TGGTATC 1605 0.0 25.488607 2 TAAGGTG 1415 0.0 25.200949 5 GTGGTAT 1895 0.0 22.84309 1 GTAAGGT 1400 0.0 22.074833 4 AGAGTAC 8700 0.0 21.126863 10-11 GCAGAGT 10155 0.0 21.093077 9 AGGTAAG 1375 0.0 20.757317 2 AAGGTAA 1435 0.0 20.552395 1 GGTAAGG 1565 0.0 20.0517 3 TACATGG 5845 0.0 19.857656 2 GTACATG 6050 0.0 19.813803 1 >>END_MODULE