FastQCFastQC Report
Thu 26 May 2016
SRR938464_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938464_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283914
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46830.20504274679344317No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38610.16905189950234553No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31090.13612596621413942No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA30930.13542541444204992No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA27630.12097653414270414No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26330.11528455099447703No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24530.10740334355847025No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA32250.055.473351
GTATCAA58150.041.759081
TCAACGC74200.032.6036454
TATCAAC74350.032.4739462
ATCAACG74400.032.4521183
CAACGCA75800.031.9774445
AACGCAG78550.031.0306076
ACGCAGA89400.026.9947137
CGCAGAG89550.026.9494938
TGGTATC14500.026.8780732
GTGGTAT16950.025.3311941
TAAGGTG14800.023.7636325
GTACATG64100.023.0720671
TACATGG61950.023.0161292
GCAGAGT101800.022.912229
GTGTAAG10300.021.7692531
GAGTACT63350.021.63260712-13
GTAAGGT14800.021.5161914
ACATGGG63900.021.3468283
CAGAGTA92900.021.14337210-11
Produced by FastQC (version 0.11.5)