##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938464_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2283914 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.805805297397363 31.0 31.0 34.0 27.0 34.0 2 30.997717076912704 31.0 31.0 34.0 27.0 34.0 3 31.04142581550794 31.0 31.0 34.0 27.0 34.0 4 34.70932618303491 37.0 35.0 37.0 32.0 37.0 5 34.68607005342583 37.0 35.0 37.0 32.0 37.0 6 34.64990363034685 37.0 35.0 37.0 30.0 37.0 7 34.54929782820194 37.0 35.0 37.0 30.0 37.0 8 34.48994139008737 37.0 35.0 37.0 30.0 37.0 9 35.93594636225357 39.0 35.0 39.0 30.0 39.0 10-11 35.752807242304215 38.0 35.0 39.0 30.0 39.0 12-13 35.550239632490545 38.0 35.0 39.0 30.0 39.0 14-15 36.66621510267024 39.0 36.0 41.0 30.0 41.0 16-17 36.574371232892304 39.0 36.0 41.0 30.0 41.0 18-19 36.55373013169498 39.0 36.0 41.0 30.0 41.0 20-21 36.461249197649295 39.0 36.0 41.0 29.0 41.0 22-23 36.296764895700974 39.0 35.5 41.0 28.5 41.0 24-25 36.1410232609459 39.0 35.0 41.0 27.5 41.0 26-27 35.90927657521255 39.0 35.0 40.0 27.0 41.0 28-29 35.65486331797082 38.5 34.5 40.0 27.0 41.0 30-31 35.35683283170908 38.0 34.0 40.0 26.5 41.0 32-33 35.038479995306304 38.0 34.0 40.0 25.0 41.0 34-35 34.71470204219598 38.0 33.5 40.0 24.5 41.0 36-37 34.459216283975664 38.0 33.0 40.0 24.0 41.0 38-39 34.18148209608593 38.0 33.0 40.0 23.5 41.0 40-41 33.87359769238246 38.0 33.0 40.0 22.0 41.0 42-43 33.42429093214543 37.5 32.0 40.0 19.0 41.0 44-45 32.94502069692642 37.0 31.0 40.0 16.5 41.0 46-47 32.457136739824705 37.0 30.5 40.0 14.0 41.0 48-49 31.942659618532048 36.5 30.0 39.0 9.0 40.5 50-51 31.213680769065736 35.5 29.5 38.5 8.0 40.0 52-53 31.207895743885278 36.0 30.0 39.0 7.5 40.0 54-55 31.15285492360921 36.0 29.5 39.0 3.5 40.0 56-57 30.695840123577334 35.5 28.5 39.0 2.0 40.0 58-59 30.207108060986535 35.0 28.0 39.0 2.0 40.0 60-61 29.617961753376 34.5 27.0 38.5 2.0 40.0 62-63 28.85119536024561 34.0 26.0 38.0 2.0 40.0 64-65 28.15863710279809 34.0 25.0 38.0 2.0 40.0 66-67 27.431622425362775 33.0 22.5 37.0 2.0 39.0 68-69 26.558661140480773 32.5 21.0 36.0 2.0 39.0 70-71 25.687511000852048 31.5 17.5 36.0 2.0 39.0 72-73 24.760912188462438 31.0 10.5 35.0 2.0 38.0 74-75 23.807755677315345 30.5 4.5 35.0 2.0 37.5 76-77 22.844734740449947 29.5 2.0 34.0 2.0 36.5 78-79 21.839207386968162 29.0 2.0 34.0 2.0 36.0 80-81 20.869641545172016 27.5 2.0 34.0 2.0 35.5 82-83 19.854101336565215 26.0 2.0 33.0 2.0 35.0 84-85 18.87220994310644 25.0 2.0 32.5 2.0 35.0 86-87 17.884631382792875 22.5 2.0 32.0 2.0 34.5 88-89 16.791574025992222 19.0 2.0 31.5 2.0 34.0 90-91 15.65306968651184 8.5 2.0 31.0 2.0 34.0 92-93 14.520857177634534 2.0 2.0 30.5 2.0 34.0 94-95 13.302970908711973 2.0 2.0 29.5 2.0 34.0 96-97 11.953822692097864 2.0 2.0 29.0 2.0 34.0 98-99 10.353543084371829 2.0 2.0 26.0 2.0 33.0 100-101 7.356612157900868 2.0 2.0 2.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6816219077487524 1101 2 0.5891333866829207 1101 3 0.6696226355431705 1101 4 0.41406598113746185 1101 5 0.49959232352784966 1101 6 0.5912603828078247 1101 7 0.49429403012113937 1101 8 0.5520676457798928 1101 9 0.5637632644426986 1101 10-11 0.6824964000797209 1101 12-13 0.7338067870838856 1101 14-15 0.861405014053723 1101 16-17 0.8563145047157761 1101 18-19 0.7301113664883303 1101 20-21 0.8597315310692579 1101 22-23 0.9219149066894801 1101 24-25 0.998349006583986 1101 26-27 1.0030053404979213 1101 28-29 1.121662279473881 1101 30-31 1.193517247817681 1101 32-33 1.2133303897652823 1101 34-35 1.4536064091974836 1101 36-37 1.3923762552607428 1101 38-39 1.5181517700151232 1101 40-41 1.56465078835177 1101 42-43 1.5852391543335074 1101 44-45 1.7071024018716656 1101 46-47 1.7009586138432624 1101 48-49 1.5964690721222077 1101 50-51 1.7304463359721076 1101 52-53 1.779616522044801 1101 54-55 1.9540372125073446 1101 56-57 2.0560328624551296 1101 58-59 2.153967266251339 1101 60-61 2.0188577848827762 1101 62-63 1.954970053453419 1101 64-65 1.9686215704116279 1101 66-67 2.2045503769404817 1101 68-69 2.333087536145264 1101 70-71 2.611096193713177 1101 72-73 2.7710076460592994 1101 74-75 2.724612122110109 1101 76-77 3.165524741794936 1101 78-79 3.2302645825722784 1101 80-81 3.4970579169674494 1101 82-83 3.6526381338524843 1101 84-85 3.6881568729144654 1101 86-87 3.565797627541947 1101 88-89 3.9190532931349225 1101 90-91 3.9360285418547445 1101 92-93 4.090263006904419 1101 94-95 4.251273959730801 1101 96-97 3.8707275685185962 1101 98-99 3.400375669470927 1101 100-101 2.5197794481740834 1102 1 0.6755143821067549 1102 2 0.5079105015201222 1102 3 0.5507523247122599 1102 4 0.2992383849914262 1102 5 0.39657581290114763 1102 6 0.5112594394931733 1102 7 0.5402299598407083 1102 8 0.7359725142442741 1102 9 0.7962208873421872 1102 10-11 0.7478458326497517 1102 12-13 0.7324595066687536 1102 14-15 0.8298745035021327 1102 16-17 0.9733433867394297 1102 18-19 0.8400813289042262 1102 20-21 0.9253255266968239 1102 22-23 0.6861054597426346 1102 24-25 0.9136867350648359 1102 26-27 0.9055207588885352 1102 28-29 0.8338205135189796 1102 30-31 0.713000844187313 1102 32-33 0.8583147945031726 1102 34-35 0.7710125734093509 1102 36-37 1.1287521277176182 1102 38-39 1.2984653609956283 1102 40-41 1.3710893912328999 1102 42-43 1.5167040695793048 1102 44-45 1.4248504303003742 1102 46-47 1.4727325869186174 1102 48-49 1.547850809725258 1102 50-51 1.5591390852888587 1102 52-53 1.6165372897282388 1102 54-55 1.5096091450533606 1102 56-57 1.913345603579021 1102 58-59 2.038129537333763 1102 60-61 1.9678355986462641 1102 62-63 2.0780555832687106 1102 64-65 2.411986662004857 1102 66-67 2.4781349900810277 1102 68-69 2.4687202426110737 1102 70-71 2.488601982433405 1102 72-73 2.6872806098753195 1102 74-75 2.7283417087374 1102 76-77 2.6809081948331546 1102 78-79 3.2899718143892294 1102 80-81 3.4670729575954873 1102 82-83 3.729779436273045 1102 84-85 4.006735192923479 1102 86-87 3.8998085315605557 1102 88-89 3.833295073414231 1102 90-91 3.7455372845473303 1102 92-93 3.937028092732948 1102 94-95 3.6950238014198913 1102 96-97 3.3596768120763247 1102 98-99 2.736012917822091 1102 100-101 1.8275727557060142 1103 1 0.5373136536615561 1103 2 0.44180099300357867 1103 3 0.37345618594846997 1103 4 0.37403850517892323 1103 5 0.3619627494052011 1103 6 0.3217372869452788 1103 7 0.46155779337590985 1103 8 0.3629682268191061 1103 9 0.4523339793528649 1103 10-11 0.5748807423516595 1103 12-13 0.5535642017270277 1103 14-15 0.6945584452775933 1103 16-17 0.5911150066575317 1103 18-19 0.7380181385389761 1103 20-21 0.7954187571274076 1103 22-23 0.7880424617454764 1103 24-25 0.69539682783563 1103 26-27 0.7013258576644432 1103 28-29 0.8913072924684613 1103 30-31 1.0539890373827632 1103 32-33 1.1390932639170686 1103 34-35 0.9872272885471034 1103 36-37 1.1322749748334928 1103 38-39 1.1108058167718227 1103 40-41 1.369327648480116 1103 42-43 1.3417869964002733 1103 44-45 1.6149122349344012 1103 46-47 1.7784205965131932 1103 48-49 1.7751505234713214 1103 50-51 1.8840262392710052 1103 52-53 1.9260166957023763 1103 54-55 1.9079138799471451 1103 56-57 2.072372731736177 1103 58-59 2.2023088958968238 1103 60-61 2.4272727432227974 1103 62-63 2.6084295264145787 1103 64-65 2.803333162054649 1103 66-67 2.9641342529537305 1103 68-69 2.9790119356290496 1103 70-71 2.9636879735248947 1103 72-73 3.153909214329918 1103 74-75 2.963177187201964 1103 76-77 3.0975276915948164 1103 78-79 3.1326044994307374 1103 80-81 3.346243451053695 1103 82-83 3.6310305523910245 1103 84-85 3.7158246279145537 1103 86-87 3.4806682467025603 1103 88-89 3.669242116010146 1103 90-91 3.7027638984177482 1103 92-93 3.478401064675296 1103 94-95 3.4902092366016344 1103 96-97 3.3126162135732216 1103 98-99 3.3176667897406897 1103 100-101 2.414743378544533 1104 1 0.7443244518932559 1104 2 0.6779867451354455 1104 3 0.6150958100637176 1104 4 0.6084426698660508 1104 5 0.6035101738538202 1104 6 0.7522070623646044 1104 7 0.7287077366066868 1104 8 0.6969508497989665 1104 9 0.7054293113924572 1104 10-11 0.8184901781219125 1104 12-13 0.8514945406513945 1104 14-15 0.9025160012214499 1104 16-17 0.9849080365237768 1104 18-19 0.9142195525356058 1104 20-21 0.8742983902613162 1104 22-23 1.0304271316384614 1104 24-25 1.1930322486123615 1104 26-27 1.2689124408011665 1104 28-29 1.3330419345807556 1104 30-31 1.1717916254153309 1104 32-33 1.2169536679851305 1104 34-35 1.2599673901680504 1104 36-37 1.3782334890097019 1104 38-39 1.413325001549623 1104 40-41 1.3829520616634525 1104 42-43 1.4026401855424524 1104 44-45 1.5265334191163262 1104 46-47 1.5820177926770427 1104 48-49 1.40239934983801 1104 50-51 1.416295719973494 1104 52-53 1.5016608855129014 1104 54-55 1.9512817445384485 1104 56-57 2.0657355635945827 1104 58-59 2.152419213401881 1104 60-61 2.0698623459992582 1104 62-63 2.1600021547895274 1104 64-65 2.351042060731647 1104 66-67 2.6450348303088482 1104 68-69 2.7575386735323733 1104 70-71 2.586022034255887 1104 72-73 2.9764270704662295 1104 74-75 2.8260595742473065 1104 76-77 3.0651929866740097 1104 78-79 3.396173955170873 1104 80-81 3.4476912676528535 1104 82-83 3.4624261493536714 1104 84-85 3.7173368634947686 1104 86-87 3.6467039428704346 1104 88-89 3.496644742694098 1104 90-91 3.162233891251139 1104 92-93 3.361581268722418 1104 94-95 3.375833134433128 1104 96-97 3.2019144090365153 1104 98-99 3.1845673107291983 1104 100-101 2.1252742714721338 1105 1 0.6965295244072536 1105 2 0.5641232085472616 1105 3 0.5619830610014311 1105 4 0.6140462343353477 1105 5 0.6056509349619787 1105 6 0.5545496149497708 1105 7 0.5438024322635471 1105 8 0.5675786369474665 1105 9 0.5890976801933334 1105 10-11 0.7297259968792744 1105 12-13 0.726121380458224 1105 14-15 0.838810162361959 1105 16-17 0.8419097001481646 1105 18-19 0.8491211984454807 1105 20-21 0.8145363424629224 1105 22-23 0.7457892780220021 1105 24-25 0.9184140735158905 1105 26-27 0.7508118608432142 1105 28-29 0.7349443198792471 1105 30-31 0.8614540482994073 1105 32-33 1.0131173193325864 1105 34-35 1.1007078955709702 1105 36-37 1.1791504079157136 1105 38-39 1.438252795629893 1105 40-41 1.402133997475282 1105 42-43 1.4958537918334187 1105 44-45 1.6116863416268572 1105 46-47 1.7468431121160322 1105 48-49 1.6175256235504385 1105 50-51 1.4545286581960362 1105 52-53 1.5365236365006858 1105 54-55 1.8825842623107292 1105 56-57 2.0397356560181628 1105 58-59 2.0814178809619754 1105 60-61 2.0594722029737724 1105 62-63 1.971019815394751 1105 64-65 2.1809070067793854 1105 66-67 2.472160380036584 1105 68-69 2.712841091179115 1105 70-71 2.72524428985329 1105 72-73 2.7093573984929087 1105 74-75 2.937849549369961 1105 76-77 3.14650686484433 1105 78-79 3.1654219353306097 1105 80-81 3.3766079092881363 1105 82-83 3.773745149330562 1105 84-85 3.8612650195673837 1105 86-87 3.801438533164422 1105 88-89 3.6145240837139916 1105 90-91 3.3846978268010997 1105 92-93 3.542409923112153 1105 94-95 3.8301471974506054 1105 96-97 3.5771828225087905 1105 98-99 3.1960218652860686 1105 100-101 2.3466194966390743 1106 1 0.3667207132896877 1106 2 0.2943421800634809 1106 3 0.20893552412552907 1106 4 0.20431892896885984 1106 5 0.2259561303896831 1106 6 -0.08106478916160853 1106 7 0.4333322516012359 1106 8 0.1653906135559211 1106 9 0.3714273235868788 1106 10-11 0.36661562012195503 1106 12-13 0.5381614303090672 1106 14-15 0.6010179794702637 1106 16-17 0.0384483373705109 1106 18-19 0.6280563040025697 1106 20-21 0.5652916240769486 1106 22-23 0.4656280146828635 1106 24-25 0.6103223344320483 1106 26-27 0.558190236630594 1106 28-29 0.6251920139018452 1106 30-31 0.3951397398670551 1106 32-33 0.6410883035859669 1106 34-35 0.47862934853881 1106 36-37 0.7816538525487573 1106 38-39 0.9431831792670877 1106 40-41 0.9894702481731414 1106 42-43 0.45552489281897124 1106 44-45 0.8858999972237029 1106 46-47 1.1731371756927658 1106 48-49 0.9384378826845854 1106 50-51 1.0066988240083568 1106 52-53 1.2111789382279774 1106 54-55 1.1648241478718404 1106 56-57 1.6755044811962243 1106 58-59 1.8304644742057938 1106 60-61 2.0286072570258433 1106 62-63 2.0065751015201627 1106 64-65 2.1416539799197025 1106 66-67 2.261857590682709 1106 68-69 2.203226664510794 1106 70-71 2.073992943485802 1106 72-73 2.179156002594137 1106 74-75 2.4749925622631324 1106 76-77 2.383997818570645 1106 78-79 2.7844057638828126 1106 80-81 2.5820640576526657 1106 82-83 2.9475018388737304 1106 84-85 2.902537268701291 1106 86-87 2.6764402719287155 1106 88-89 2.7373832889201353 1106 90-91 2.753983542429486 1106 92-93 2.9165708518566547 1106 94-95 2.599326989438003 1106 96-97 2.2556506234137004 1106 98-99 2.1933571385164274 1106 100-101 1.4772047365039578 1107 1 0.1824636322948301 1107 2 0.241509826726098 1107 3 0.24452744257131087 1107 4 0.18367469203197828 1107 5 0.2255892898484504 1107 6 0.15184719498002863 1107 7 0.1994908548237575 1107 8 0.22280062298564474 1107 9 0.2233629159513839 1107 10-11 0.27160552576221164 1107 12-13 0.2263362647981424 1107 14-15 0.3749854152352583 1107 16-17 0.28563480780603356 1107 18-19 0.39416521603310173 1107 20-21 0.5627520205537735 1107 22-23 0.5456041593422185 1107 24-25 0.4009423859431607 1107 26-27 0.5548173912165879 1107 28-29 0.6942241418892294 1107 30-31 0.6929488668097648 1107 32-33 0.7731726662049851 1107 34-35 0.7622173380795729 1107 36-37 0.5587661073601922 1107 38-39 0.6558926160437437 1107 40-41 0.8378086264560309 1107 42-43 0.8694001545452394 1107 44-45 0.718447460341153 1107 46-47 0.9369955317082983 1107 48-49 0.843359897845307 1107 50-51 1.061623292504411 1107 52-53 0.9747412881842159 1107 54-55 1.0898004397313201 1107 56-57 1.1926742600302696 1107 58-59 1.2529384532391425 1107 60-61 1.5451213262348809 1107 62-63 1.5757089314085668 1107 64-65 1.7131833356381634 1107 66-67 1.979202493053343 1107 68-69 1.9896673923752424 1107 70-71 2.036854353234734 1107 72-73 2.004624388915051 1107 74-75 2.163407466765939 1107 76-77 2.1536225831619653 1107 78-79 2.50112546719874 1107 80-81 2.8006089532431737 1107 82-83 2.818071717447012 1107 84-85 2.9432575501122358 1107 86-87 2.7034812015911456 1107 88-89 3.0401757198007573 1107 90-91 2.7858752959219206 1107 92-93 2.6905245489437846 1107 94-95 2.690950945766849 1107 96-97 2.5777492867474 1107 98-99 2.2912482514653103 1107 100-101 1.57845112006465 1108 1 0.18548211849972063 1108 2 0.13705122048310514 1108 3 0.05580377466548825 1108 4 0.11880387629552303 1108 5 -0.05706233833689822 1108 6 0.09951968652931242 1108 7 -0.11029713968011379 1108 8 0.07920116585459169 1108 9 0.1467964777280173 1108 10-11 0.28240836486563836 1108 12-13 0.08617306692915605 1108 14-15 0.04558535285590892 1108 16-17 0.14896889980714434 1108 18-19 0.08973788376190583 1108 20-21 0.021373521198640333 1108 22-23 0.05859491202495093 1108 24-25 0.08186374581301692 1108 26-27 0.08035094540655052 1108 28-29 0.1389025088989797 1108 30-31 0.22433090661453292 1108 32-33 0.22120687369218928 1108 34-35 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34-35 -0.9247096996239605 2315 36-37 -0.9248508213448474 2315 38-39 -1.1625677046112486 2315 40-41 -0.980564298744639 2315 42-43 -0.9806083630173816 2315 44-45 -1.0711375830819776 2315 46-47 -0.9276758548061927 2315 48-49 -1.0701349633348691 2315 50-51 -0.8905415496279936 2315 52-53 -0.8889287092111822 2315 54-55 -0.8285795231465798 2315 56-57 -0.7516718542510823 2315 58-59 -0.5071997483251955 2315 60-61 -0.6918696589610036 2315 62-63 -0.4092854417382874 2315 64-65 -0.4449323065531736 2315 66-67 -0.4513855377651055 2315 68-69 -0.6004921590024317 2315 70-71 -0.5367669855287929 2315 72-73 -0.6180141250695357 2315 74-75 -0.5049188091583652 2315 76-77 -0.42496434662174565 2315 78-79 -0.6213479573481528 2315 80-81 -0.5563953114576776 2315 82-83 -0.5646587105753085 2315 84-85 -0.4368994394193848 2315 86-87 -0.5092089143529428 2315 88-89 -0.6695317693401748 2315 90-91 -0.5690457012182826 2315 92-93 -0.7202454321399898 2315 94-95 -0.6203173785030085 2315 96-97 -0.8522211385650849 2315 98-99 -1.0255998162647693 2315 100-101 -0.4951462938849476 2316 1 -1.3261858453538267 2316 2 -1.080735095970411 2316 3 -0.8149710145757822 2316 4 -0.9121093705768928 2316 5 -0.6973159861629483 2316 6 -0.5924345323320566 2316 7 -0.7439280886474151 2316 8 -0.659677081550349 2316 9 -0.8453138951244554 2316 10-11 -0.9101635061147917 2316 12-13 -0.9886651670396631 2316 14-15 -1.2097141334580854 2316 16-17 -1.1250187618974365 2316 18-19 -1.1594316981615904 2316 20-21 -1.0997974698794408 2316 22-23 -1.2004206460248383 2316 24-25 -1.146947075183391 2316 26-27 -1.2232802493435315 2316 28-29 -1.230195632163948 2316 30-31 -1.190707886100462 2316 32-33 -1.2881603175841576 2316 34-35 -1.1819233279215027 2316 36-37 -1.5398491305445887 2316 38-39 -1.4638684701698068 2316 40-41 -1.3925291396879587 2316 42-43 -1.2509936928800443 2316 44-45 -1.3920851120205526 2316 46-47 -0.9328140977035524 2316 48-49 -0.7767645783565875 2316 50-51 -0.6881645849117035 2316 52-53 -0.7529982768521428 2316 54-55 -0.9702064980907714 2316 56-57 -0.8201403152329085 2316 58-59 -0.5661540219267245 2316 60-61 -0.9288222104393178 2316 62-63 -0.8579308997821506 2316 64-65 -0.7964008576683987 2316 66-67 -0.6533322113952664 2316 68-69 -0.5454222512836928 2316 70-71 -0.6302199768069485 2316 72-73 -0.5016807548731315 2316 74-75 -0.5394259968173749 2316 76-77 -0.3719650299735342 2316 78-79 -0.4919120038390865 2316 80-81 -0.7977898093571234 2316 82-83 -0.5232860428568706 2316 84-85 -0.3808953565682529 2316 86-87 -0.24650106418149775 2316 88-89 -0.4051614054569441 2316 90-91 -0.2219564506167533 2316 92-93 -0.2114160348447598 2316 94-95 -0.29115572457434347 2316 96-97 -0.15971657755947888 2316 98-99 -0.27859467418640804 2316 100-101 0.3358001291615862 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10194.0 3 4041.0 4 4626.0 5 5152.0 6 5908.0 7 7428.0 8 9494.0 9 12320.0 10 15083.0 11 18825.0 12 22213.0 13 25434.0 14 29210.0 15 32404.0 16 36851.0 17 40539.0 18 41681.0 19 42844.0 20 46209.0 21 50928.0 22 55770.0 23 61739.0 24 67725.0 25 74001.0 26 80745.0 27 87331.0 28 95683.0 29 104881.0 30 114766.0 31 126595.0 32 140223.0 33 154290.0 34 169644.0 35 178932.0 36 168501.0 37 113680.0 38 27659.0 39 365.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.245974343928495 18.277126889206823 14.35351051274782 24.12338825411686 2 15.491566741018737 19.44762821571239 40.217607907217726 24.84319713605115 3 19.357323967288924 22.97054803010414 29.591952490012662 28.080175512594273 4 12.959714266566271 16.018015153088065 36.56123807308647 34.46103250725919 5 14.03147898662636 36.85700829143802 34.31008006659438 14.801432655341241 6 33.406404888259225 35.49152886895922 16.91943817261534 14.182628070166217 7 29.20711091722107 32.113797101373095 20.19389588017245 18.485196101233377 8 26.605300446996626 35.10463137561514 19.441352772539823 18.848715404848413 9 26.338862772013478 16.591338229864224 19.73174345728836 37.33805554083394 10-11 25.219966203519984 26.095713768868166 27.31459459554163 21.369725432070215 12-13 26.10372219576155 23.276977570472077 27.437493498885914 23.18180673488046 14-15 22.567573367622945 24.97746678485838 25.637608965285274 26.817350882233406 16-17 22.640001436439942 28.07167134604495 26.541753323847573 22.74657389366753 18-19 22.614597635455468 26.705294714045145 28.476951274791258 22.203156375708126 20-21 22.95928758418701 25.955856814302624 28.837607159547662 22.2472484419627 22-23 22.669905708493683 25.88431820366764 28.704544593507624 22.741231494331057 24-25 22.56865113850596 26.104252664923926 28.777148583963502 22.549947612606612 26-27 22.1839145152435 26.463758702179756 28.449812438721477 22.902514343855263 28-29 22.30283446061507 26.94605559426792 28.2978520257128 22.45325791940421 30-31 22.415099892977675 26.363012629159872 28.253718461865606 22.968169015996846 32-33 22.072150590378754 26.358352895834063 28.295653621106474 23.273842892680705 34-35 22.89345139316824 26.55840427390968 28.073000360451267 22.475143972470807 36-37 22.979849851678054 26.039512808507677 28.111596441674852 22.869040898139417 38-39 22.422339114601535 26.483064764041753 27.999936065356966 23.094660055999743 40-41 22.998605076941907 26.255118750187474 28.127328384067795 22.618947788802828 42-43 22.696767817117756 26.22567367522891 27.939406505878956 23.13815200177438 44-45 22.6072016529834 26.58477506301923 28.107067327282 22.700955956715376 46-47 23.06807342936947 26.766124532833267 27.407414057525337 22.75838798027192 48-49 22.699647645848607 26.8044684076798 27.41627100177009 23.079612944701505 50-51 22.967935683509406 26.95660379713217 27.377334782186985 22.698125737171438 52-53 23.24115332944488 26.59722185731476 27.462599904365053 22.69902490887531 54-55 22.666581425832483 26.883309749792613 27.65997927509239 22.79012954928252 56-57 22.781328661973852 26.948835520898403 27.44857202346977 22.821263793657977 58-59 22.73927113114766 26.54065446740372 27.421478820742294 23.298595580706326 60-61 22.338517276734038 26.751543130477696 27.73034970326224 23.179589889526024 62-63 22.545381376092372 26.79771161426482 27.812745430114532 22.84416157952828 64-65 22.324015002685307 26.826134238171022 27.621720570935764 23.228130188207903 66-67 22.17694000197038 26.841110855691657 27.59186890414108 23.390080238196884 68-69 22.869587777268144 26.379649506136214 27.681549537991334 23.069213178604308 70-71 22.575865690703527 26.416667889138516 27.305715103336826 23.70175131682113 72-73 22.58916846164726 26.58175412261684 27.348498018332666 23.48057939740323 74-75 22.560349474548936 26.860863956364728 27.285752632728993 23.293033936357343 76-77 22.672486777004433 26.467008683532818 27.330917628493317 23.52958691096943 78-79 22.759682339227584 26.641638503232045 27.241894054107075 23.356785103433296 80-81 22.92194242950384 26.542420307973906 27.002695264348286 23.532941998173964 82-83 22.806474772415413 26.62211423490482 27.12220148743115 23.449209505248618 84-85 22.74305148840143 26.624194812954453 27.10142297057615 23.531330728067964 86-87 22.946750492532058 26.592418147948116 26.73384528790384 23.72698607161599 88-89 22.812664696205772 26.589704980446104 26.603192689661604 23.994437633686516 90-91 22.733248779428177 26.571568340635448 26.6915671494064 24.003615730529972 92-93 22.891743530639914 26.16292155495847 26.876416568287787 24.06891834611383 94-95 22.683103390287737 25.928786265077967 27.047459473852008 24.340650870782284 96-97 22.84969315817282 25.59109229209214 27.03799049312559 24.52122405660945 98-99 22.72299133191602 25.707273177107854 26.556106083860808 25.013629407115317 100-101 23.57795856600392 24.77587783555636 26.808846699124377 24.837316899315336 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 152.0 1 109.5 2 90.5 3 130.5 4 136.5 5 139.5 6 225.0 7 447.5 8 748.5 9 970.5 10 960.0 11 857.5 12 851.0 13 952.5 14 1132.0 15 1314.0 16 1449.0 17 1672.5 18 2010.0 19 2409.5 20 2835.0 21 3412.0 22 4282.0 23 5358.5 24 6894.0 25 8865.5 26 11558.0 27 14874.5 28 17901.5 29 20895.0 30 24334.5 31 29575.0 32 36474.0 33 40107.5 34 41526.5 35 44262.5 36 48127.5 37 52545.0 38 56541.5 39 59962.5 40 63938.0 41 68608.0 42 71689.0 43 73947.0 44 76489.0 45 80125.5 46 83159.5 47 85577.0 48 87164.5 49 86862.5 50 85362.5 51 83737.5 52 82524.5 53 81107.0 54 80125.5 55 76250.0 56 70613.0 57 65258.0 58 60467.5 59 53815.5 60 44477.5 61 37285.5 62 31803.0 63 25424.0 64 19169.0 65 14724.0 66 11198.5 67 8514.5 68 6717.0 69 5312.5 70 4178.5 71 3091.5 72 2243.0 73 1726.0 74 1285.5 75 911.5 76 651.0 77 462.0 78 324.5 79 216.0 80 128.0 81 84.0 82 59.0 83 35.5 84 19.5 85 10.5 86 8.5 87 4.0 88 3.0 89 3.0 90 2.5 91 0.0 92 1.0 93 2.0 94 1.0 95 0.0 96 0.0 97 0.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3861353798785769 2 0.02771557948329053 3 0.03384540748907358 4 0.04146390801054681 5 0.06300587500229868 6 0.05341707262182376 7 0.03647247663440918 8 0.03936225269427833 9 0.03507137309023019 10-11 0.036603830091675955 12-13 0.028525592469769 14-15 0.027058812196956626 16-17 0.021498182505996288 18-19 0.026226906967600355 20-21 0.03296971777396172 22-23 0.03189699787294968 24-25 0.040369295866656975 26-27 0.03474298944706324 28-29 0.030342648628626123 30-31 0.032641334130794765 32-33 0.027759363969046123 34-35 0.02911668302746951 36-37 0.030868062457693243 38-39 0.01453644927085696 40-41 0.01206262582566594 42-43 0.013244806941066958 44-45 0.012500470683221871 46-47 0.02049113933361764 48-49 0.018214346074326794 50-51 0.01179991891113238 52-53 0.010070431723786447 54-55 0.008253375564929328 56-57 0.00921663425155238 58-59 0.003831142503614409 60-61 0.007158763421039497 62-63 0.012938315540777803 64-65 0.008297160050684921 66-67 0.0038968192322477993 68-69 0.005976582305638478 70-71 0.012675608626244245 72-73 0.01011421620954204 74-75 0.011252612839187465 76-77 0.004750616704481867 78-79 0.009632586866230515 80-81 0.012215871525810516 82-83 0.01368265179862289 84-85 0.008997711822774413 86-87 0.0022986855021686456 88-89 0.015477815714602213 90-91 0.024431743051621033 92-93 0.009895293780764074 94-95 0.01970301859001696 96-97 0.017885962431159842 98-99 0.01090233695314272 100-101 0.12224628422961635 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2283914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.40743955396458 #Duplication Level Percentage of deduplicated Percentage of total 1 77.83523180485825 45.461565968110804 2 12.628097667003043 14.751497023340828 3 4.237872203705818 7.425697937261226 4 1.9199832527820087 4.485652231259578 5 0.9782649809026992 2.856897636991736 6 0.5805318391239702 2.0344426981671107 7 0.3556226973884061 1.4539707841211813 8 0.2579573631179975 1.2053303275051626 9 0.18658486914931743 0.9808150019870834 >10 0.9238026974189154 9.542287550946137 >50 0.05741294036191493 2.296712767512343 >100 0.03320914484556815 3.5885589239648206 >500 0.0027142696710655938 1.0967878863121272 >1k 0.0027142696710647928 2.8197832625199846 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4683 0.20504274679344317 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3861 0.16905189950234553 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3109 0.13612596621413942 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3093 0.13542541444204992 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2763 0.12097653414270414 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2633 0.11528455099447703 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2453 0.10740334355847025 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 2.1892242877796623E-5 0.0 0.0 0.0 0.0 14-15 4.3784485755593247E-5 0.0 0.0 0.0 0.0 16-17 4.3784485755593247E-5 0.0 0.0 0.0 0.0 18-19 6.567672863338987E-5 0.0 0.0 0.0 0.0 20-21 8.756897151118649E-5 0.0 0.0 0.0 0.0 22-23 8.756897151118649E-5 0.0 0.0 6.567672863338987E-5 0.0 24-25 1.3135345726677974E-4 0.0 0.0 2.627069145335595E-4 0.0 26-27 1.3135345726677974E-4 0.0 0.0 6.348750434561021E-4 0.0 28-29 1.970301859001696E-4 0.0 0.0 0.003130590731524918 0.0 30-31 2.1892242877796623E-4 0.0 0.0 0.011427750782209837 0.0 32-33 2.1892242877796623E-4 0.0 0.0 0.03001426498545917 0.0 34-35 2.4081467165576286E-4 0.0 0.0 0.05880256436976174 0.0 36-37 2.627069145335595E-4 0.0 0.0 0.10775361944451499 0.0 38-39 2.627069145335595E-4 0.0 0.0 0.17881583982584282 0.0 40-41 2.627069145335595E-4 0.0 0.0 0.2553730131694976 0.0 42-43 2.845991574113561E-4 0.0 0.0 0.33696540237504563 0.0 44-45 3.940603718003392E-4 0.0 0.0 0.4390051464284557 0.0 46-47 4.1595261467813584E-4 0.0 0.0 0.5583178701124474 0.0 48-49 4.3784485755593247E-4 0.0 0.0 0.6834539304019328 0.0 50-51 4.3784485755593247E-4 0.0 0.0 0.8197550345590946 0.0 52-53 4.5973710043372914E-4 0.0 0.0 0.9503641555680293 0.0 54-55 4.8162934331152577E-4 0.0 0.0 1.0976113811640893 0.0 56-57 5.691983148227122E-4 0.0 0.0 1.2669916643096018 0.0 58-59 5.691983148227122E-4 0.0 0.0 1.4534041124140402 0.0 60-61 5.691983148227122E-4 0.0 0.0 1.6574179237922269 0.0 62-63 5.691983148227122E-4 0.0 0.0 1.8570095021090987 0.0 64-65 5.691983148227122E-4 0.0 0.0 2.065160947391189 0.0 66-67 5.691983148227122E-4 0.0 0.0 2.312083554809857 0.0 68-69 5.691983148227122E-4 0.0 0.0 2.585495776110659 0.0 70-71 5.691983148227122E-4 0.0 0.0 2.8703795326794266 0.0 72-73 5.910905577005089E-4 0.0 0.0 3.163516664813123 0.0 74-75 6.129828005783055E-4 0.0 0.0 3.4554497235885413 2.1892242877796623E-5 76-77 6.129828005783055E-4 2.1892242877796623E-5 0.0 3.772952922045226 4.3784485755593247E-5 78-79 6.129828005783055E-4 4.3784485755593247E-5 0.0 4.120251463058592 4.3784485755593247E-5 80-81 6.129828005783055E-4 4.3784485755593247E-5 0.0 4.490493074607888 4.3784485755593247E-5 82-83 6.129828005783055E-4 4.3784485755593247E-5 0.0 4.865310164918644 4.3784485755593247E-5 84-85 6.34875043456102E-4 4.3784485755593247E-5 0.0 5.2487527989232525 4.3784485755593247E-5 86-87 6.567672863338987E-4 4.3784485755593247E-5 0.0 5.647279188270661 4.3784485755593247E-5 88-89 6.567672863338987E-4 4.3784485755593247E-5 0.0 6.060210673431662 4.3784485755593247E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3225 0.0 55.47335 1 GTATCAA 5815 0.0 41.75908 1 TCAACGC 7420 0.0 32.603645 4 TATCAAC 7435 0.0 32.473946 2 ATCAACG 7440 0.0 32.452118 3 CAACGCA 7580 0.0 31.977444 5 AACGCAG 7855 0.0 31.030607 6 ACGCAGA 8940 0.0 26.994713 7 CGCAGAG 8955 0.0 26.949493 8 TGGTATC 1450 0.0 26.878073 2 GTGGTAT 1695 0.0 25.331194 1 TAAGGTG 1480 0.0 23.763632 5 GTACATG 6410 0.0 23.072067 1 TACATGG 6195 0.0 23.016129 2 GCAGAGT 10180 0.0 22.91222 9 GTGTAAG 1030 0.0 21.769253 1 GAGTACT 6335 0.0 21.632607 12-13 GTAAGGT 1480 0.0 21.516191 4 ACATGGG 6390 0.0 21.346828 3 CAGAGTA 9290 0.0 21.143372 10-11 >>END_MODULE