FastQCFastQC Report
Thu 26 May 2016
SRR938464_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938464_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2283914
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47060.20604978996582182No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43380.18993709920776353No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA31340.13722057835802925No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA30670.13428701781240449No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA29820.13056533652317906No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC27290.11948786162701398No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26310.11519698202296584No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA26000.11383966296454244No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG25230.11046825756136176No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA31800.052.6005331
GTATCAA57650.039.8952031
TATCAAC72050.031.453822
ATCAACG72450.031.2719463
TCAACGC72900.031.0789074
CAACGCA75200.030.1283555
AACGCAG76750.029.5199016
ACGCAGA89250.025.3854627
CGCAGAG89500.025.2614828
GTACATG65250.023.0134181
TACATGG61500.022.9440632
ACATGGG63000.022.1657123
GTGGTAT17600.021.329911
GCAGAGT103400.021.3143629
TGGTATC15850.020.6827752
TAAGGTG16100.020.6512835
GTGTAGC12900.020.6287521
AGAGTAC90800.020.24417110-11
GAGTACT66500.020.17770212-13
CAGAGTA95800.019.8073410-11
Produced by FastQC (version 0.11.5)