##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938464_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2283914 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78309516032565 31.0 31.0 34.0 30.0 34.0 2 32.03982768177786 31.0 31.0 34.0 30.0 34.0 3 32.10009571288586 33.0 31.0 34.0 30.0 34.0 4 35.760163473756016 37.0 35.0 37.0 35.0 37.0 5 35.64679931030678 37.0 35.0 37.0 33.0 37.0 6 35.67616381352363 37.0 35.0 37.0 33.0 37.0 7 35.59580483328181 37.0 35.0 37.0 33.0 37.0 8 35.559602944769374 37.0 35.0 37.0 33.0 37.0 9 37.102567784951624 39.0 37.0 39.0 33.0 39.0 10-11 37.026929866886405 39.0 37.0 39.0 33.0 39.0 12-13 36.91581272324615 39.0 37.0 39.0 33.0 39.0 14-15 37.93903404418906 40.0 37.0 41.0 33.0 41.0 16-17 38.01510761788754 40.0 37.0 41.0 33.0 41.0 18-19 38.14272844774365 40.0 37.0 41.0 33.0 41.0 20-21 38.085682516942406 40.0 37.0 41.0 33.0 41.0 22-23 37.96440890506385 40.0 37.0 41.0 33.0 41.0 24-25 37.85009571288586 40.0 37.0 41.0 32.0 41.0 26-27 37.633212108687104 40.0 37.0 41.0 32.0 41.0 28-29 37.491052640335845 39.5 36.5 41.0 32.0 41.0 30-31 37.22853312340132 39.0 36.0 41.0 31.0 41.0 32-33 37.12519385581068 39.0 36.0 41.0 31.0 41.0 34-35 37.00135534875656 39.0 36.0 41.0 31.0 41.0 36-37 36.874161417636564 39.0 36.0 41.0 30.0 41.0 38-39 36.648867470491446 39.0 35.0 40.0 30.0 41.0 40-41 36.4263028292659 39.0 35.0 40.0 30.0 41.0 42-43 36.15717185498228 38.5 35.0 40.0 30.0 41.0 44-45 35.87977174271886 38.0 35.0 40.0 29.0 41.0 46-47 35.49060319258956 38.0 34.0 40.0 27.5 41.0 48-49 35.089109090797635 38.0 34.0 40.0 26.5 41.0 50-51 34.68759375353012 38.0 33.0 40.0 25.5 41.0 52-53 34.07435656508958 37.5 33.0 40.0 24.0 41.0 54-55 33.633800353253235 37.0 32.0 40.0 23.0 41.0 56-57 33.239403278757436 37.0 32.0 39.0 22.0 40.5 58-59 32.699922807951616 36.0 31.0 39.0 20.0 40.0 60-61 32.196763538381916 35.5 31.0 39.0 18.0 40.0 62-63 31.669829293046938 35.0 30.0 38.5 15.5 40.0 64-65 31.005299236310996 35.0 29.0 38.0 10.0 40.0 66-67 30.361519960909213 34.0 28.5 38.0 7.5 40.0 68-69 29.63290824435596 34.0 28.0 37.0 6.0 39.0 70-71 28.843925384230754 33.5 26.0 36.0 2.0 39.0 72-73 28.25818507176715 33.0 26.0 36.0 2.0 38.5 74-75 27.547993926216137 32.0 25.0 35.0 2.0 38.0 76-77 26.415572784264207 30.5 24.0 34.0 2.0 36.0 78-79 26.909858033183383 32.0 25.0 35.0 2.0 37.0 80-81 26.534738173153627 32.0 24.0 35.0 2.0 36.0 82-83 25.855589133391188 31.5 22.0 34.5 2.0 36.0 84-85 25.067680744546422 31.0 19.5 34.0 2.0 35.0 86-87 24.16162232903691 30.5 15.0 34.0 2.0 35.0 88-89 23.175156989273678 30.0 7.0 34.0 2.0 35.0 90-91 22.314191121031705 29.0 2.0 34.0 2.0 35.0 92-93 21.238508323868587 29.0 2.0 33.5 2.0 35.0 94-95 20.062610720018355 27.0 2.0 33.0 2.0 34.5 96-97 18.69916008220975 25.5 2.0 32.5 2.0 34.0 98-99 16.928037351669108 12.5 2.0 32.0 2.0 34.0 100-101 12.718643521603703 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.30388667713031836 1101 2 0.2165028388325041 1101 3 0.2107259678640574 1101 4 0.19874548236313672 1101 5 0.15354383481589196 1101 6 0.20777610822543835 1101 7 0.26852778289973855 1101 8 0.26091255406682734 1101 9 0.26650954977319685 1101 10-11 0.32736217984779614 1101 12-13 0.33127046075242106 1101 14-15 0.6277265286115608 1101 16-17 0.39900056308816545 1101 18-19 0.503714966961283 1101 20-21 0.45230842210710875 1101 22-23 0.44548498595131036 1101 24-25 0.4197083488343907 1101 26-27 0.4577044247866553 1101 28-29 0.5871663000019822 1101 30-31 0.657598045970758 1101 32-33 0.5157920753241001 1101 34-35 0.372519181099662 1101 36-37 0.43721724251071237 1101 38-39 0.5936169044899557 1101 40-41 0.6060447009752821 1101 42-43 0.7774838455559987 1101 44-45 0.731660295825705 1101 46-47 0.7065439662283453 1101 48-49 0.828972704560222 1101 50-51 0.9705278887737805 1101 52-53 1.0000881270087731 1101 54-55 0.9840033618687016 1101 56-57 1.2346979784659737 1101 58-59 1.3132905429590735 1101 60-61 1.225697028749586 1101 62-63 1.1904558155032312 1101 64-65 1.3073268083495542 1101 66-67 1.5656844699790682 1101 68-69 1.4869490801504526 1101 70-71 1.7713579635278158 1101 72-73 1.9642598424770448 1101 74-75 2.0556184879859067 1101 76-77 2.2612778230531383 1101 78-79 2.1004777099474516 1101 80-81 2.359095711858192 1101 82-83 2.559618533675266 1101 84-85 2.714706642515406 1101 86-87 2.846517012369489 1101 88-89 2.5443101819387053 1101 90-91 3.1013681956676287 1101 92-93 3.1673944491949904 1101 94-95 3.0212884037911074 1101 96-97 3.0161608272368277 1101 98-99 3.08415526196384 1101 100-101 2.231046959825864 1102 1 0.2189263443848084 1102 2 0.19172074014956308 1102 3 0.17155788404181038 1102 4 0.1598403038277354 1102 5 0.12744677297899898 1102 6 0.21364693176533223 1102 7 0.16163363765198824 1102 8 0.1712325910307868 1102 9 0.19938092095965487 1102 10-11 0.26323403404629175 1102 12-13 0.24614111746537048 1102 14-15 0.39165286621863515 1102 16-17 0.2377197426839217 1102 18-19 0.33773242177608154 1102 20-21 0.2517147452849571 1102 22-23 0.207471632808371 1102 24-25 0.22751890679626285 1102 26-27 0.2523013717662579 1102 28-29 0.23605104878768657 1102 30-31 0.3066015825272643 1102 32-33 0.39420907979349096 1102 34-35 0.31897122621455765 1102 36-37 0.39756439977087155 1102 38-39 0.4530313593894775 1102 40-41 0.6133285312520158 1102 42-43 0.5355236377123376 1102 44-45 0.5270029417708884 1102 46-47 0.5041306965862091 1102 48-49 0.5540320766096372 1102 50-51 0.8615922970752976 1102 52-53 0.7925182889706548 1102 54-55 0.8944011349473726 1102 56-57 1.0447296930538954 1102 58-59 1.2225685666100645 1102 60-61 1.2124272992800016 1102 62-63 1.311992955460557 1102 64-65 1.542646255741861 1102 66-67 1.5633744408847967 1102 68-69 1.7143852994152091 1102 70-71 1.8938178923075561 1102 72-73 1.770973360438017 1102 74-75 1.620096883023372 1102 76-77 1.5683656098360785 1102 78-79 1.8859437204863703 1102 80-81 2.0131599568265237 1102 82-83 2.0818248592987487 1102 84-85 1.9726641129996985 1102 86-87 1.9400222036571044 1102 88-89 2.0348477752111265 1102 90-91 2.162410261386963 1102 92-93 2.336249300146907 1102 94-95 2.1222224163109864 1102 96-97 2.2513536259029117 1102 98-99 2.2214597316157487 1102 100-101 1.9407158913211315 1103 1 0.20108079894039932 1103 2 0.21048726912266602 1103 3 0.3064398372759385 1103 4 0.21717578442026308 1103 5 0.1649802565183336 1103 6 0.1551257487185751 1103 7 0.19917180271573187 1103 8 0.13002113168046492 1103 9 0.2156879472468347 1103 10-11 0.3300845001984456 1103 12-13 0.3872720545959041 1103 14-15 0.3978160930997703 1103 16-17 0.2763697697942078 1103 18-19 0.24925161049343103 1103 20-21 0.2299107581582973 1103 22-23 0.33844025723179527 1103 24-25 0.3410524703471083 1103 26-27 0.444339630886077 1103 28-29 0.41750374283473946 1103 30-31 0.3985107592514012 1103 32-33 0.4606156523132867 1103 34-35 0.5016353711000789 1103 36-37 0.4935816141617124 1103 38-39 0.38251887716862853 1103 40-41 0.42791931069177735 1103 42-43 0.3786453292577576 1103 44-45 0.6351197687868648 1103 46-47 0.6695257619190116 1103 48-49 0.6820313377799678 1103 50-51 0.6503095678997823 1103 52-53 0.7762393518299149 1103 54-55 0.8766211308446543 1103 56-57 0.9049121363159429 1103 58-59 0.9434043719301499 1103 60-61 0.9084493228731816 1103 62-63 0.8572667012163286 1103 64-65 1.222439774979943 1103 66-67 1.2711639016668137 1103 68-69 0.91569297685988 1103 70-71 1.0623176595081176 1103 72-73 1.0522721174507303 1103 74-75 0.9409836744001758 1103 76-77 1.1674417748334278 1103 78-79 1.328153493636723 1103 80-81 1.432366421415903 1103 82-83 1.3695913550755883 1103 84-85 1.6099827996874048 1103 86-87 1.8068002764182083 1103 88-89 1.4986908726438628 1103 90-91 1.754411366226723 1103 92-93 1.7178205644393891 1103 94-95 1.6103890121873299 1103 96-97 1.5155735434910547 1103 98-99 1.7368862057213832 1103 100-101 1.883999435933081 1104 1 0.2227066534984452 1104 2 0.21872927512973916 1104 3 0.2451818150997056 1104 4 0.18382889625966925 1104 5 0.2024233866366245 1104 6 0.1176085367720745 1104 7 0.04238647091771952 1104 8 0.11653421275980946 1104 9 0.2197705001710588 1104 10-11 0.27266142048800646 1104 12-13 0.31830267776901877 1104 14-15 0.46883359090550414 1104 16-17 0.4659884496882327 1104 18-19 0.32669510296849325 1104 20-21 0.31984992248306554 1104 22-23 0.3443744532426507 1104 24-25 0.24142529519995293 1104 26-27 0.44342387480855194 1104 28-29 0.4195512598856368 1104 30-31 0.47449979191988234 1104 32-33 0.5853102660812368 1104 34-35 0.5091665539130972 1104 36-37 0.5520156692560434 1104 38-39 0.5611482828230052 1104 40-41 0.675005559655041 1104 42-43 0.6921618406478416 1104 44-45 0.7309717903350048 1104 46-47 0.8194454395020117 1104 48-49 0.9338533883971891 1104 50-51 0.9446105011697696 1104 52-53 0.9081134610361943 1104 54-55 0.8513936513178137 1104 56-57 1.1116845843177572 1104 58-59 1.2263107953077963 1104 60-61 1.2484559258187389 1104 62-63 1.2666863667207657 1104 64-65 1.4403653630044566 1104 66-67 1.3331383836979462 1104 68-69 1.4242810335807938 1104 70-71 1.5340039068608995 1104 72-73 1.838495111806015 1104 74-75 1.7308543935575749 1104 76-77 1.6987407433244925 1104 78-79 1.9666826875136998 1104 80-81 2.0891904046025473 1104 82-83 2.0255144790740722 1104 84-85 2.1563501730839754 1104 86-87 2.14144359657249 1104 88-89 2.1269665778303946 1104 90-91 2.398056635055184 1104 92-93 2.720892544598339 1104 94-95 2.4488286234187804 1104 96-97 2.743220559684378 1104 98-99 2.7860666754968015 1104 100-101 2.2152154649860396 1105 1 0.13380942081438718 1105 2 0.11440577720541967 1105 3 0.16543143577793984 1105 4 0.16653991083948938 1105 5 0.04128326479153799 1105 6 0.08744494704033912 1105 7 0.11928827856884539 1105 8 0.12326881075575358 1105 9 0.2118182215447817 1105 10-11 0.19600145453069473 1105 12-13 0.16449713697556234 1105 14-15 0.3278110010428321 1105 16-17 0.24970437812157797 1105 18-19 0.23324350596610088 1105 20-21 0.23881928406720476 1105 22-23 0.2529390380682415 1105 24-25 0.3207701660765139 1105 26-27 0.3001401806381665 1105 28-29 0.2932985353155999 1105 30-31 0.2643374345024938 1105 32-33 0.39235140227507515 1105 34-35 0.40251725517698134 1105 36-37 0.3522883188089452 1105 38-39 0.26150372849719616 1105 40-41 0.4327025181491493 1105 42-43 0.4228824749816624 1105 44-45 0.2759333575308034 1105 46-47 0.3719587831559821 1105 48-49 0.5579106679091907 1105 50-51 0.7284771955635065 1105 52-53 0.5762797002438091 1105 54-55 0.593756575599798 1105 56-57 0.664826600846169 1105 58-59 0.76676305467916 1105 60-61 0.7417790925585805 1105 62-63 0.8479798346844483 1105 64-65 1.1387427515762774 1105 66-67 1.1523314873586905 1105 68-69 1.281950457550849 1105 70-71 1.3701596251846446 1105 72-73 1.3566068596128815 1105 74-75 1.17448814202681 1105 76-77 1.2325163619848105 1105 78-79 1.43610569482286 1105 80-81 1.5822528598348775 1105 82-83 1.8335054600733294 1105 84-85 2.112221958476429 1105 86-87 2.091447447554877 1105 88-89 1.8608873785266553 1105 90-91 1.9641577849585232 1105 92-93 1.9792068155688654 1105 94-95 1.9864407832807593 1105 96-97 2.0704054145408506 1105 98-99 1.9724401668744527 1105 100-101 1.9498829711668684 1106 1 0.08343506956144253 1106 2 0.11631428248843889 1106 3 0.0852676347828023 1106 4 0.07639278318411158 1106 5 0.14032835423921597 1106 6 0.05243431708133528 1106 7 0.011223795790137103 1106 8 -0.01216664161432135 1106 9 0.031966834198847494 1106 10-11 0.1546660459586775 1106 12-13 0.12761287100665442 1106 14-15 0.11914227417543799 1106 16-17 0.04728157455682691 1106 18-19 0.1995171179152635 1106 20-21 0.22222047620159913 1106 22-23 0.23235874193318296 1106 24-25 0.1596519432628014 1106 26-27 -0.05484969693403485 1106 28-29 0.276086648558973 1106 30-31 0.25635246494497466 1106 32-33 0.14860944993413483 1106 34-35 0.05955981448764902 1106 36-37 0.1271260498061224 1106 38-39 0.2635346051915448 1106 40-41 0.1936689515394363 1106 42-43 0.30532292935366456 1106 44-45 0.291210422571865 1106 46-47 -0.0841789608114496 1106 48-49 0.17366922967855203 1106 50-51 0.4436318891932416 1106 52-53 0.4487583510379096 1106 54-55 0.3003677333912478 1106 56-57 0.49024113165236827 1106 58-59 0.6946836617616725 1106 60-61 0.596769158105289 1106 62-63 0.5471590439508454 1106 64-65 0.6906372527576998 1106 66-67 0.8514209387926535 1106 68-69 0.6378406390778402 1106 70-71 0.636450436802221 1106 72-73 0.9976987471965977 1106 74-75 0.8157284354926304 1106 76-77 0.936678681740748 1106 78-79 1.3479987486004923 1106 80-81 1.2936142276878506 1106 82-83 1.7212230170813 1106 84-85 1.8359527075360838 1106 86-87 1.705789245380764 1106 88-89 1.8221609622796393 1106 90-91 1.9561335287266992 1106 92-93 2.130297349736054 1106 94-95 2.0347476967955664 1106 96-97 1.9643572404313332 1106 98-99 2.082659213580481 1106 100-101 1.8702924924521618 1107 1 0.045833021963169074 1107 2 0.010861722410716368 1107 3 0.005525672132378645 1107 4 0.05810285604371046 1107 5 0.0025679549968060655 1107 6 0.003009691125761549 1107 7 -0.05008318069263851 1107 8 0.017756555607967073 1107 9 0.06404789596669502 1107 10-11 0.027802826986459195 1107 12-13 0.05151246089820205 1107 14-15 0.16628619039449433 1107 16-17 0.09301459992966699 1107 18-19 0.09731473648496092 1107 20-21 0.13689427627529227 1107 22-23 0.15159208802386104 1107 24-25 0.15219353621789367 1107 26-27 0.23866600396603133 1107 28-29 0.20570714880743424 1107 30-31 0.15450969786819968 1107 32-33 0.15530054289948225 1107 34-35 0.3280605054360919 1107 36-37 0.3447967551088098 1107 38-39 0.374188860657803 1107 40-41 0.3435168893549658 1107 42-43 0.28293075790210054 1107 44-45 0.3463361537773011 1107 46-47 0.33535645776770906 1107 48-49 0.4225429703369272 1107 50-51 0.3860378350075351 1107 52-53 0.37305627337512703 1107 54-55 0.40097027254870454 1107 56-57 0.472663920728877 1107 58-59 0.5390072236834058 1107 60-61 0.7483550386697928 1107 62-63 1.0481956896437232 1107 64-65 1.097029306449162 1107 66-67 1.0244243475905819 1107 68-69 1.2267095444352982 1107 70-71 0.8982587858145976 1107 72-73 1.1156201387640827 1107 74-75 1.0860819744957517 1107 76-77 1.0553217107445256 1107 78-79 1.0654151099384244 1107 80-81 1.4155155536486888 1107 82-83 1.5662152243961636 1107 84-85 1.914144162256882 1107 86-87 2.0168976801260605 1107 88-89 1.8532057352033817 1107 90-91 1.9228557745901504 1107 92-93 2.100298589785549 1107 94-95 1.9536675512369506 1107 96-97 2.099083711384292 1107 98-99 1.9850339529693564 1107 100-101 1.7287300479309948 1108 1 -0.013386898966789573 1108 2 -0.02236337431942559 1108 3 -0.060667923025633286 1108 4 -0.047048835363952435 1108 5 -0.011250082751132595 1108 6 -0.043036216704535946 1108 7 -0.03859239850616092 1108 8 0.009342592748971867 1108 9 -0.042830186121157965 1108 10-11 -0.04338878720568573 1108 12-13 -0.059748613543966655 1108 14-15 -0.055798881552512114 1108 16-17 0.026558252830731988 1108 18-19 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1987.0 10 3263.0 11 5016.0 12 7418.0 13 10002.0 14 13797.0 15 17822.0 16 22642.0 17 27481.0 18 27978.0 19 26895.0 20 28418.0 21 31235.0 22 34159.0 23 38036.0 24 43094.0 25 48801.0 26 56632.0 27 65735.0 28 77466.0 29 90426.0 30 104457.0 31 121672.0 32 142607.0 33 168539.0 34 203484.0 35 246211.0 36 281395.0 37 249025.0 38 84476.0 39 2001.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.033644889282456 19.11063345622144 14.97732745082067 23.87839420367543 2 16.022446620307928 20.194470231735302 39.14049499921178 24.642588148744988 3 19.423628196489034 23.188811286792216 30.164418074034767 27.223142442683983 4 13.523232485986775 16.710436557593674 36.16594144963427 33.600389506785284 5 13.913257451701208 37.07509234172305 34.52695993008475 14.484690276490998 6 33.397360846336596 36.02460512961521 16.590598420080617 13.987435603967574 7 29.253597114427247 32.152699269762344 20.13473361956711 18.45896999624329 8 26.589410367207506 35.50113336191002 19.27459583618115 18.634860434701324 9 26.11267519298046 16.529241520024872 19.660188010911114 37.69789527608356 10-11 25.243310387343833 26.298604063025138 27.29476679069352 21.163318758937507 12-13 26.016675759244873 23.358235029865398 27.484769566629918 23.14031964425981 14-15 22.425275207385216 25.11127827054784 25.589952161070862 26.87349436099608 16-17 22.512844178896405 28.193180653912535 26.63353348681255 22.660441680378508 18-19 22.53950893072156 26.80061508445589 28.59199164241736 22.067884342405186 20-21 22.832580386126622 26.060416460514713 28.89184969311454 22.215153460244125 22-23 22.646846597551395 25.963696531480608 28.811921114367706 22.57753575660029 24-25 22.5947376659599 26.17394359798982 28.846903669097113 22.384415066953167 26-27 22.164659878727903 26.578381863701892 28.505401852032275 22.751556405537922 28-29 22.30728342741902 27.023907674160686 28.315464131483232 22.353344766937067 30-31 22.436943772839083 26.441538516774276 28.314813955341577 22.806703755045067 32-33 22.108751872614693 26.402471555850276 28.36067429234703 23.128102279187996 34-35 22.90978723366988 26.587142939090825 28.11262003109894 22.390449796140352 36-37 22.999482240181447 26.080317355024512 28.225638196444425 22.694562208349616 38-39 22.380966039279244 26.505003999126824 28.193767492758763 22.920262468835165 40-41 23.005895871138605 26.26276913267987 28.216936879497222 22.5143981166843 42-43 22.719280634856553 26.302182138781056 28.002030833375112 22.976506392987275 44-45 22.614867987495916 26.609296799522397 28.21510722807346 22.560727984908226 46-47 23.050546234547873 26.8321329790285 27.51258916182738 22.60473162459625 48-49 22.770822368968357 26.83632133258958 27.479537320582125 22.913318977859937 50-51 22.951455264953058 27.033701794375798 27.477238635079953 22.53760430559119 52-53 23.339254455290348 26.640298189861788 27.441182111060225 22.57926524378764 54-55 22.737546159794107 26.917979398523762 27.668511161103265 22.675963280578866 56-57 22.81740906181231 26.95237211208478 27.48275548028516 22.74746334581775 58-59 22.703810213519425 26.66308801469758 27.433213334652706 23.199888437130294 60-61 22.378491484355365 26.87958040451611 27.717878168792698 23.024049942335832 62-63 22.584825873478596 26.940353270744872 27.750475718437734 22.724345137338798 64-65 22.282856530498897 26.926887021185813 27.592981423746682 23.197275024568604 66-67 22.166223966411845 26.970745879691343 27.554724910301477 23.30830524359533 68-69 22.842798286520306 26.562897827194433 27.544643668336988 23.04966021794828 70-71 22.54709351888623 26.679453201588675 27.20502050647288 23.568432773052216 72-73 22.52599708394814 26.813469007097478 27.243783686747726 23.41675022220665 74-75 22.52816874891086 27.09596333312025 27.08760049634093 23.28826742162796 76-77 22.527227382467117 26.738244084497055 27.396105982974838 23.33842255006099 78-79 22.5887664771966 26.96784992779938 27.35247474291939 23.090908852084624 80-81 22.773715181170548 26.91989159825151 27.075501830959738 23.230891389618204 82-83 22.695966436153117 27.04066665323926 27.199037079784194 23.06432983082343 84-85 22.547707502430722 27.087930504887385 27.106189654768514 23.258172337913376 86-87 22.799842719240708 27.107877436603857 26.708676801175578 23.383603042979857 88-89 22.626793303075335 27.25382829651204 26.476193937249825 23.643184463162797 90-91 22.436195230837335 27.140293211423888 26.815998731982972 23.60751282575581 92-93 22.565304960812647 26.87070362099917 26.888173737904463 23.675817680283725 94-95 22.353624472752117 26.692058777233708 27.03526384450536 23.919052905508817 96-97 22.548511578919253 26.34767034741027 27.022345636119756 24.081472437550723 98-99 22.30583113024396 26.613699114765264 26.671560312691284 24.408909442299493 100-101 22.59512017420975 26.28282550981024 26.875142162783312 24.246912153196696 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 152.0 1 119.5 2 107.0 3 133.5 4 123.5 5 132.5 6 250.0 7 532.0 8 889.0 9 1077.0 10 967.5 11 827.0 12 880.0 13 1006.5 14 1145.5 15 1338.5 16 1521.0 17 1713.5 18 1945.0 19 2330.0 20 2790.0 21 3308.0 22 4127.5 23 5285.5 24 6726.0 25 8615.0 26 11461.5 27 15041.0 28 18148.0 29 21162.5 30 24632.5 31 29924.0 32 37868.5 33 41318.0 34 41728.5 35 44781.5 36 48748.0 37 53400.5 38 57355.5 39 60767.5 40 65110.0 41 69725.0 42 72655.0 43 75046.0 44 78140.5 45 81947.5 46 84412.5 47 87176.5 48 89087.5 49 88247.5 50 86844.5 51 84658.0 52 82482.5 53 82096.0 54 82554.0 55 77515.0 56 70629.0 57 64441.5 58 58884.5 59 52180.5 60 42472.0 61 35356.5 62 29745.0 63 22599.5 64 16097.5 65 11901.5 66 9006.0 67 7082.5 68 5515.5 69 4297.0 70 3364.0 71 2319.5 72 1674.0 73 1320.5 74 942.0 75 682.0 76 501.5 77 335.5 78 210.0 79 137.0 80 91.5 81 52.5 82 31.5 83 16.0 84 10.0 85 7.5 86 4.0 87 0.5 88 1.5 89 2.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.02346848436499798 2 0.01199694909703255 3 0.0017951639159793232 4 0.0 5 2.627069145335595E-4 6 0.0 7 0.0 8 4.3784485755593247E-5 9 1.7513794302237299E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.00181705615885712 26-27 0.009041496308530007 28-29 1.3135345726677974E-4 30-31 0.0 32-33 3.721681289225426E-4 34-35 0.010289354152564413 36-37 0.0015981337300791537 38-39 0.012719393111999837 40-41 0.012347224983077297 42-43 0.005363599505060173 44-45 8.975819579896616E-4 46-47 2.1892242877796623E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 1.5324570014457636E-4 66-67 5.910905577005089E-4 68-69 0.0010289354152564413 70-71 4.3784485755593247E-5 72-73 1.7513794302237299E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.0946121438898312E-4 82-83 0.001225965601156611 84-85 0.005582521933838139 86-87 0.004378448575559324 88-89 0.0 90-91 0.0014448880299345772 92-93 6.129828005783055E-4 94-95 1.0946121438898312E-4 96-97 2.1892242877796623E-5 98-99 0.0 100-101 2.1892242877796623E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2283914.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.58560788014396 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76884002556062 40.813025832884655 2 14.237578566013518 15.543337615343125 3 4.83996693312092 7.925776114926045 4 2.1725814970089528 4.743667267335474 5 1.1494120831368784 3.1370678631404534 6 0.667602493740832 2.186489276588599 7 0.43369574268601496 1.657148202468264 8 0.3010828976718278 1.3147834393385522 9 0.21654989424101465 1.0638456852174005 >10 1.0969803618262775 10.66863349492595 >50 0.07041054220723827 2.6390945536566823 >100 0.03911931546652411 4.057295375850574 >500 0.003210206399693335 1.2353790276232703 >1k 0.0029694409197158667 3.0144562507012442 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4706 0.20604978996582182 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4338 0.18993709920776353 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3134 0.13722057835802925 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3067 0.13428701781240449 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2982 0.13056533652317906 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2729 0.11948786162701398 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2631 0.11519698202296584 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2600 0.11383966296454244 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2523 0.11046825756136176 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 4.3784485755593247E-5 0.0 0.0 0.0 0.0 6 4.3784485755593247E-5 0.0 0.0 0.0 0.0 7 4.3784485755593247E-5 0.0 0.0 0.0 0.0 8 4.3784485755593247E-5 0.0 0.0 0.0 0.0 9 4.3784485755593247E-5 0.0 0.0 0.0 0.0 10-11 4.3784485755593247E-5 0.0 0.0 0.0 0.0 12-13 4.3784485755593247E-5 0.0 0.0 0.0 0.0 14-15 4.3784485755593247E-5 0.0 0.0 0.0 0.0 16-17 6.567672863338987E-5 0.0 0.0 4.3784485755593247E-5 0.0 18-19 8.756897151118649E-5 0.0 0.0 4.3784485755593247E-5 0.0 20-21 8.756897151118649E-5 0.0 0.0 4.3784485755593247E-5 0.0 22-23 8.756897151118649E-5 0.0 0.0 1.0946121438898312E-4 0.0 24-25 8.756897151118649E-5 0.0 0.0 3.064914002891527E-4 0.0 26-27 8.756897151118649E-5 0.0 0.0 6.786595292116954E-4 0.0 28-29 1.7513794302237299E-4 0.0 0.0 0.0031743752172805105 0.0 30-31 2.1892242877796623E-4 0.0 0.0 0.0116685654538656 0.0 32-33 2.4081467165576286E-4 0.0 0.0 0.030364540871503917 0.0 34-35 2.845991574113561E-4 0.0 0.0 0.059349870441706656 0.0 36-37 3.064914002891527E-4 0.0 0.0 0.10906715401718278 0.0 38-39 3.064914002891527E-4 0.0 0.0 0.1808956028992335 0.0 40-41 3.2838364316694935E-4 0.0 0.0 0.25778115988605527 0.0 42-43 4.597371004337291E-4 0.0 0.0 0.33948301030599226 0.0 44-45 5.691983148227122E-4 0.0 0.0 0.44222330613149186 0.0 46-47 6.34875043456102E-4 0.0 0.0 0.5625868574736177 0.0 48-49 6.567672863338987E-4 0.0 0.0 0.6879199479490034 0.0 50-51 6.567672863338987E-4 0.0 0.0 0.8247026814494767 0.0 52-53 6.786595292116954E-4 0.0 0.0 0.9560780309591341 0.0 54-55 7.443362578450852E-4 0.0 0.0 1.1042447307560619 0.0 56-57 7.443362578450852E-4 0.0 0.0 1.2751793631458979 0.0 58-59 7.443362578450852E-4 0.0 0.0 1.4612196431214135 0.0 60-61 7.443362578450852E-4 0.0 0.0 1.6658902217859342 0.0 62-63 7.443362578450852E-4 0.0 0.0 1.8664669510323069 0.0 64-65 7.443362578450852E-4 0.0 0.0 2.075931930887065 0.0 66-67 7.881207436006784E-4 0.0 0.0 2.3243213185785456 0.0 68-69 7.881207436006784E-4 0.0 0.0 2.6009735918252614 0.0 70-71 8.537974722340684E-4 0.0 0.0 2.891417978084989 0.0 72-73 8.756897151118649E-4 0.0 0.0 3.1923706409260593 2.1892242877796623E-5 74-75 8.756897151118649E-4 0.0 0.0 3.4936079029245413 4.3784485755593247E-5 76-77 8.756897151118649E-4 0.0 0.0 3.8235458953358137 4.3784485755593247E-5 78-79 8.756897151118649E-4 0.0 0.0 4.185753053748959 4.3784485755593247E-5 80-81 9.194742008674583E-4 0.0 0.0 4.575040916601939 4.3784485755593247E-5 82-83 9.194742008674583E-4 0.0 0.0 4.971618896333224 4.3784485755593247E-5 84-85 9.194742008674583E-4 0.0 0.0 5.379953010489887 4.3784485755593247E-5 86-87 9.194742008674583E-4 0.0 0.0 5.806545255206633 4.3784485755593247E-5 88-89 9.194742008674583E-4 0.0 0.0 6.256518415316863 4.3784485755593247E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3180 0.0 52.600533 1 GTATCAA 5765 0.0 39.895203 1 TATCAAC 7205 0.0 31.45382 2 ATCAACG 7245 0.0 31.271946 3 TCAACGC 7290 0.0 31.078907 4 CAACGCA 7520 0.0 30.128355 5 AACGCAG 7675 0.0 29.519901 6 ACGCAGA 8925 0.0 25.385462 7 CGCAGAG 8950 0.0 25.261482 8 GTACATG 6525 0.0 23.013418 1 TACATGG 6150 0.0 22.944063 2 ACATGGG 6300 0.0 22.165712 3 GTGGTAT 1760 0.0 21.32991 1 GCAGAGT 10340 0.0 21.314362 9 TGGTATC 1585 0.0 20.682775 2 TAAGGTG 1610 0.0 20.651283 5 GTGTAGC 1290 0.0 20.628752 1 AGAGTAC 9080 0.0 20.244171 10-11 GAGTACT 6650 0.0 20.177702 12-13 CAGAGTA 9580 0.0 19.80734 10-11 >>END_MODULE