FastQCFastQC Report
Thu 26 May 2016
SRR938463_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938463_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162759
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71570.33091990369708324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54720.2530101597080396No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39570.18296074597308346No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38420.17764346374237722No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA38210.17667248176981346No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36520.16885838875251474No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32330.14948498653802852No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29010.13413422392416352No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28900.1336256143194873No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27050.12507172551356854No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA27020.12493301380320229No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25900.1197544432828623No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24190.11184787579198607No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT22150.10241547948708109No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48100.043.2048031
GTATCAA83400.039.629121
TATCAAC107800.030.7055112
ATCAACG107750.030.6314873
AGGTAAG14100.030.3563292
TCAACGC108600.030.3041524
CAACGCA110150.029.7050195
AACGCAG113650.028.8916636
TAAGGTG16050.028.4460545
GTAAGGT16350.027.6332324
GGTAAGG16400.026.9690073
TACATGG94800.026.0868032
GTACATG97400.025.876921
ACGCAGA130050.025.0301937
CGCAGAG130800.024.8140098
AAGGTAA16150.024.4400021
ACATGGG98900.024.42833
GTGTAAG8450.024.1995561
TAAATCG1000.001642357923.7791235
GGACCGA7300.022.1319646
Produced by FastQC (version 0.11.5)