##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938463_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2162759 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.99778338686835 31.0 31.0 34.0 28.0 34.0 2 31.039630398024006 31.0 31.0 34.0 27.0 34.0 3 31.070337934092517 31.0 31.0 34.0 27.0 34.0 4 34.73909483211028 37.0 35.0 37.0 32.0 37.0 5 34.71196975714816 37.0 35.0 37.0 32.0 37.0 6 34.727323293996236 37.0 35.0 37.0 31.0 37.0 7 34.6314901475384 37.0 35.0 37.0 30.0 37.0 8 34.592675836743716 37.0 35.0 37.0 30.0 37.0 9 36.06503637252232 39.0 35.0 39.0 30.0 39.0 10-11 35.89219487700664 38.0 35.0 39.0 30.0 39.0 12-13 35.752714241392596 38.0 35.0 39.0 30.0 39.0 14-15 36.68187370853618 39.0 36.0 41.0 30.0 41.0 16-17 36.51780202972222 39.0 36.0 40.5 30.0 41.0 18-19 36.663109481916386 39.0 36.0 41.0 30.0 41.0 20-21 36.6309242037601 39.0 36.0 41.0 30.0 41.0 22-23 36.54662539839159 39.0 36.0 41.0 30.0 41.0 24-25 36.39333508726585 39.0 35.5 41.0 29.0 41.0 26-27 36.17525692876552 39.0 35.0 40.5 28.5 41.0 28-29 35.96929015206965 39.0 35.0 40.0 27.0 41.0 30-31 35.70062406398493 38.0 34.5 40.0 27.0 41.0 32-33 35.40331146466157 38.0 34.0 40.0 26.5 41.0 34-35 35.13857114916641 38.0 34.0 40.0 25.5 41.0 36-37 34.839837448370346 38.0 33.5 40.0 25.0 41.0 38-39 34.67648244672661 38.0 33.0 40.0 24.0 41.0 40-41 34.558595525437646 38.0 33.0 40.0 24.0 41.0 42-43 34.23375443126118 38.0 33.0 40.0 23.5 41.0 44-45 33.868920670310466 38.0 33.0 40.0 22.5 41.0 46-47 33.45031531483628 38.0 32.5 40.0 19.5 41.0 48-49 33.05304751939536 37.0 31.5 40.0 17.5 41.0 50-51 32.34205151845398 36.5 30.5 39.5 15.0 40.5 52-53 32.36169864511025 36.5 31.0 39.0 14.5 40.0 54-55 32.45492493615794 37.0 31.0 40.0 12.5 41.0 56-57 32.12802512901345 36.0 31.0 39.0 8.5 41.0 58-59 31.746964178625543 36.0 30.5 39.0 7.5 41.0 60-61 31.270517658231917 35.0 30.0 39.0 6.0 40.0 62-63 30.701014537449616 35.0 29.0 38.5 2.0 40.0 64-65 30.17559029924277 35.0 28.0 38.0 2.0 40.0 66-67 29.64822340353225 34.0 28.0 38.0 2.0 40.0 68-69 29.078290970006364 34.0 26.5 37.0 2.0 39.5 70-71 28.439751955719522 34.0 26.0 36.5 2.0 39.0 72-73 27.8243928241658 33.0 25.5 36.0 2.0 39.0 74-75 27.18043341861021 33.0 24.5 35.0 2.0 38.0 76-77 26.46094733624967 32.0 22.5 35.0 2.0 37.0 78-79 25.698755154873936 31.5 20.0 35.0 2.0 36.5 80-81 25.036717452106316 31.0 18.0 35.0 2.0 36.0 82-83 24.31432928957873 31.0 13.0 34.0 2.0 35.5 84-85 23.628472936651747 30.5 7.0 34.0 2.0 35.0 86-87 22.903013696856654 30.0 2.0 34.0 2.0 35.0 88-89 22.07745476957904 29.0 2.0 34.0 2.0 35.0 90-91 21.18688351314224 29.0 2.0 34.0 2.0 35.0 92-93 20.26021530831683 27.0 2.0 33.5 2.0 35.0 94-95 19.27629569452722 25.5 2.0 33.0 2.0 35.0 96-97 18.059879764689455 24.5 2.0 32.5 2.0 34.0 98-99 16.58693779565823 10.5 2.0 32.0 2.0 34.0 100-101 13.244290048035865 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.576360866987347 1101 2 0.4700106851328272 1101 3 0.4948761826204553 1101 4 0.4381455205646745 1101 5 0.4953161993023869 1101 6 0.6854252476082365 1101 7 0.5714873908540596 1101 8 0.6050927526962298 1101 9 0.8170487062580634 1101 10-11 0.739145246910411 1101 12-13 0.7623867279759224 1101 14-15 1.0671011779554362 1101 16-17 0.8753872389876207 1101 18-19 0.9023931545963464 1101 20-21 0.9261841081565194 1101 22-23 1.0803020127181853 1101 24-25 0.943003366371201 1101 26-27 1.024542813819565 1101 28-29 1.276149858667388 1101 30-31 1.1020957551978299 1101 32-33 1.1453783153793395 1101 34-35 1.1127459156323383 1101 36-37 1.150401711009998 1101 38-39 1.1305609564567618 1101 40-41 1.115007397273061 1101 42-43 1.252280769989369 1101 44-45 1.381332293837481 1101 46-47 1.1790393456451653 1101 48-49 0.9950764551805236 1101 50-51 1.1371472208981928 1101 52-53 0.9986965765937086 1101 54-55 1.1645325190860234 1101 56-57 1.0615031341734067 1101 58-59 1.0955989563266826 1101 60-61 1.2547711036189746 1101 62-63 1.1717997030255525 1101 64-65 1.116163027883875 1101 66-67 1.1408284167266949 1101 68-69 1.2232085504038288 1101 70-71 1.435983450932767 1101 72-73 1.5571439895075976 1101 74-75 1.6149233181130747 1101 76-77 1.3381913654301947 1101 78-79 1.5586303310996463 1101 80-81 1.558816367656867 1101 82-83 1.4973718757326075 1101 84-85 1.4162583178409704 1101 86-87 1.5772240388450278 1101 88-89 1.3342501602481356 1101 90-91 1.1513566319348847 1101 92-93 1.3322428787734353 1101 94-95 1.03500397557848 1101 96-97 0.82820758637693 1101 98-99 0.9282685091679603 1101 100-101 0.9785552768016856 1102 1 0.5669416877770246 1102 2 0.4454821899970902 1102 3 0.4330775389573063 1102 4 0.42610158415153876 1102 5 0.32136371060519764 1102 6 0.5479544664563747 1102 7 0.5488477226803496 1102 8 0.438954486195378 1102 9 0.6257286798051283 1102 10-11 0.71034856434148 1102 12-13 0.6353690937251955 1102 14-15 0.8081470567460869 1102 16-17 0.9272989159207441 1102 18-19 0.6507350369556448 1102 20-21 0.7116357473235055 1102 22-23 0.7606211688445725 1102 24-25 0.8674500279358313 1102 26-27 0.7876612707732349 1102 28-29 0.661714395928179 1102 30-31 0.7597691342276462 1102 32-33 0.7326869111277361 1102 34-35 0.7262061603219934 1102 36-37 0.8578486439667827 1102 38-39 0.8700893528099201 1102 40-41 0.9318570803369397 1102 42-43 1.2087205105343202 1102 44-45 1.144972088868819 1102 46-47 1.2019600242711093 1102 48-49 1.1073696356469398 1102 50-51 1.0294662772021894 1102 52-53 0.9505041938746288 1102 54-55 1.044590707226284 1102 56-57 1.121604407116962 1102 58-59 1.0934961870869557 1102 60-61 0.8906260096819416 1102 62-63 0.8725805013551913 1102 64-65 1.0437162716959278 1102 66-67 0.9578072970105218 1102 68-69 0.981702162810091 1102 70-71 1.288785574862274 1102 72-73 1.256719900283013 1102 74-75 1.1422038180153287 1102 76-77 1.343904118157635 1102 78-79 1.3904707909897596 1102 80-81 1.5783809080371043 1102 82-83 1.4697358885098772 1102 84-85 1.69854475509635 1102 86-87 1.6210078588818781 1102 88-89 1.6220823296800226 1102 90-91 1.6387912274669993 1102 92-93 1.6071117838880866 1102 94-95 1.732183628862014 1102 96-97 1.9606554031778707 1102 98-99 1.8173420377234066 1102 100-101 1.4859058707449613 1103 1 0.6484508140772931 1103 2 0.4036462835411534 1103 3 0.46094651800897424 1103 4 0.44302931522119593 1103 5 0.3935135406815462 1103 6 0.4665126366501724 1103 7 0.3267537995295626 1103 8 0.4539766129437268 1103 9 0.7673088837909745 1103 10-11 0.710263354392147 1103 12-13 0.7863479309144026 1103 14-15 1.0090558983192537 1103 16-17 1.0419240901835636 1103 18-19 0.8698559317182131 1103 20-21 0.7537550010683276 1103 22-23 0.8917457247300788 1103 24-25 0.9241377622783276 1103 26-27 0.8217232217954233 1103 28-29 0.7574728286267245 1103 30-31 0.8774493998696613 1103 32-33 0.7920028398447698 1103 34-35 0.8737802306439306 1103 36-37 1.1095890934928363 1103 38-39 0.9488261298355738 1103 40-41 0.976229193153948 1103 42-43 0.9565337981060367 1103 44-45 0.9487324862376667 1103 46-47 0.7054397129546217 1103 48-49 0.9851366895589351 1103 50-51 1.0791388361852654 1103 52-53 1.122455813989312 1103 54-55 1.265619142899915 1103 56-57 1.2140909714203403 1103 58-59 1.2208148599516875 1103 60-61 1.2163047299253371 1103 62-63 1.106662399251885 1103 64-65 1.2770426575135048 1103 66-67 1.1901186677214888 1103 68-69 1.3144961558959842 1103 70-71 1.593832230940638 1103 72-73 1.6643005114368457 1103 74-75 1.4840488264431286 1103 76-77 1.567060139753515 1103 78-79 1.6422629232549646 1103 80-81 1.6220310359851702 1103 82-83 1.5878846878363326 1103 84-85 1.6007752949715837 1103 86-87 1.611754954057762 1103 88-89 1.5437204038092887 1103 90-91 1.4697379559980241 1103 92-93 1.4711249352691311 1103 94-95 1.7236299539771842 1103 96-97 1.7199811116731816 1103 98-99 1.6787918704867764 1103 100-101 1.5412042229733594 1104 1 0.24573419109425743 1104 2 0.21404971525762306 1104 3 0.35454495537524977 1104 4 0.21915150856508347 1104 5 0.2641559898206651 1104 6 0.4199111956624364 1104 7 0.46599016664413995 1104 8 0.5360233500808533 1104 9 0.5410148620640314 1104 10-11 0.5760852725451855 1104 12-13 0.6140709268105695 1104 14-15 0.7341041699897843 1104 16-17 0.6921691212563772 1104 18-19 0.7030972754257832 1104 20-21 0.684676856110066 1104 22-23 0.6701168584770372 1104 24-25 0.6960381581083155 1104 26-27 0.7654293465196247 1104 28-29 0.7396055600458453 1104 30-31 0.8945558089836823 1104 32-33 0.7687346131988093 1104 34-35 0.6974873759495495 1104 36-37 0.9372779937316906 1104 38-39 0.9312178967183016 1104 40-41 0.9053642298056346 1104 42-43 0.9587227850714228 1104 44-45 0.8149993641601085 1104 46-47 0.7239237925709574 1104 48-49 0.490146577299555 1104 50-51 0.7818861452598753 1104 52-53 0.7693596994754941 1104 54-55 0.7789021473817783 1104 56-57 0.8776579080605345 1104 58-59 0.919314170487155 1104 60-61 0.717627710078002 1104 62-63 0.8332466917258081 1104 64-65 0.7231201466452291 1104 66-67 0.8427831174839433 1104 68-69 0.8968298457463213 1104 70-71 1.2074891892693387 1104 72-73 1.2537822757521049 1104 74-75 0.9312117702759117 1104 76-77 1.0762075011844132 1104 78-79 1.1299902028471358 1104 80-81 1.1265782296379783 1104 82-83 1.162606579399327 1104 84-85 1.477408511281297 1104 86-87 1.5147181412740416 1104 88-89 1.5065290209946873 1104 90-91 1.4133144485754734 1104 92-93 1.6547350841504453 1104 94-95 1.7093582898482431 1104 96-97 1.763603873659406 1104 98-99 1.8438371627918215 1104 100-101 1.6496578178684391 1105 1 0.3647346321492826 1105 2 0.2969488646016458 1105 3 0.3522225454610677 1105 4 0.3970133819975601 1105 5 0.22666195017108492 1105 6 0.2950409273517778 1105 7 0.3111613121351482 1105 8 0.27891030170135167 1105 9 0.4434976397596415 1105 10-11 0.6231509914888633 1105 12-13 0.631734395282983 1105 14-15 0.6662484434072553 1105 16-17 0.602130530032376 1105 18-19 0.6177832298518453 1105 20-21 0.6542467860167847 1105 22-23 0.6275646462436342 1105 24-25 0.7836862834499527 1105 26-27 0.7274180160734431 1105 28-29 0.6002690359346232 1105 30-31 0.6864855926747637 1105 32-33 0.7569311562173837 1105 34-35 0.7950390624942258 1105 36-37 0.9594547507862714 1105 38-39 0.9075252584109066 1105 40-41 0.8752736761048965 1105 42-43 0.9599990526029245 1105 44-45 0.9578245200889839 1105 46-47 0.8360650863585377 1105 48-49 0.8963090670311686 1105 50-51 1.0468109063332633 1105 52-53 0.9369262536316896 1105 54-55 0.8584849861096373 1105 56-57 0.8811145841110744 1105 58-59 1.0430851822220006 1105 60-61 0.9599136097102807 1105 62-63 1.0757347961097032 1105 64-65 1.3339829916202746 1105 66-67 1.3648763314750276 1105 68-69 1.2161203099272875 1105 70-71 1.2091165339828507 1105 72-73 1.399827656867707 1105 74-75 1.3835270037755016 1105 76-77 1.3657111550482703 1105 78-79 1.5254373676398636 1105 80-81 1.6058923781683845 1105 82-83 1.6839150775393499 1105 84-85 1.6943037543872492 1105 86-87 1.5674976168155546 1105 88-89 1.6821794026534285 1105 90-91 1.4805541813947194 1105 92-93 1.2834417895392143 1105 94-95 1.3714300880221622 1105 96-97 1.6378567303741107 1105 98-99 2.0621595090125986 1105 100-101 1.7098521220201572 1106 1 0.2394319329771939 1106 2 0.10124836185389441 1106 3 0.054198349839484905 1106 4 -0.03254342938708277 1106 5 0.15441235234177952 1106 6 -0.005551490236285872 1106 7 0.362555373402202 1106 8 0.28651763837815025 1106 9 0.1446696317100944 1106 10-11 0.15366888663017164 1106 12-13 0.1563422907564771 1106 14-15 0.2853149842229996 1106 16-17 0.12066899633963857 1106 18-19 0.03444233234522187 1106 20-21 0.07741827143210855 1106 22-23 0.07261045128007737 1106 24-25 -0.042164125343362 1106 26-27 -0.09783127897735255 1106 28-29 0.09495570671873566 1106 30-31 -0.22459961334686085 1106 32-33 -0.11602596158504497 1106 34-35 -0.2891568510748783 1106 36-37 -0.13980233102549278 1106 38-39 0.09952333637033917 1106 40-41 0.3797861550865491 1106 42-43 -0.20505184317504188 1106 44-45 -0.07246327607484915 1106 46-47 0.12593912330174817 1106 48-49 0.20201052826018184 1106 50-51 -0.04241999603347324 1106 52-53 -0.13767754766688967 1106 54-55 -0.10275153511543067 1106 56-57 -0.7176728842013915 1106 58-59 -0.5202896831833179 1106 60-61 0.17880988786196284 1106 62-63 0.171983241222474 1106 64-65 0.25862668732088423 1106 66-67 -0.19927441710306582 1106 68-69 -0.010843291270877131 1106 70-71 -0.1729399140040968 1106 72-73 0.200956654691975 1106 74-75 0.5433657080950347 1106 76-77 0.7147907697176485 1106 78-79 0.7427492953149404 1106 80-81 0.943907035526145 1106 82-83 0.43905654662240323 1106 84-85 0.45579637841905907 1106 86-87 0.4822535098355054 1106 88-89 0.5319627499196535 1106 90-91 0.6245102376738636 1106 92-93 0.460901843827763 1106 94-95 0.6259209324102954 1106 96-97 0.5379956784187137 1106 98-99 0.7482109830530987 1106 100-101 0.4902584588296257 1107 1 0.20526854060548771 1107 2 0.06511021761087932 1107 3 0.16680668934798604 1107 4 0.15299095302247423 1107 5 0.19574092642786667 1107 6 0.23008900913523433 1107 7 0.1712395950175818 1107 8 0.022306855661497593 1107 9 0.29783613102200235 1107 10-11 0.4214180662150184 1107 12-13 0.441030615218466 1107 14-15 0.39087140196103576 1107 16-17 0.5910244907316269 1107 18-19 0.5250814288145378 1107 20-21 0.5606480914803456 1107 22-23 0.4588241877996282 1107 24-25 0.38962142486666806 1107 26-27 0.5482317046513501 1107 28-29 0.7155059116041258 1107 30-31 0.7012989891980936 1107 32-33 0.7503348073081426 1107 34-35 0.8300362900180289 1107 36-37 0.7308758822629144 1107 38-39 0.658469278899652 1107 40-41 0.5327402149210982 1107 42-43 0.8128968774089813 1107 44-45 0.5720642233778719 1107 46-47 0.48716115904132806 1107 48-49 0.547625508172473 1107 50-51 0.4601502441454457 1107 52-53 0.3823176629380143 1107 54-55 0.4494162828217725 1107 56-57 0.3696489908981775 1107 58-59 0.4756761250579373 1107 60-61 0.3605408122460467 1107 62-63 0.7307935184832282 1107 64-65 0.7142889136907158 1107 66-67 0.8535158166420267 1107 68-69 0.9159652963406266 1107 70-71 0.9691115915206652 1107 72-73 1.160757391189943 1107 74-75 1.047996929812168 1107 76-77 1.0293397392268773 1107 78-79 1.1409780413464752 1107 80-81 1.2315819486541244 1107 82-83 0.8926945855192798 1107 84-85 0.8940527508325573 1107 86-87 0.9898754312873699 1107 88-89 0.9026772433897357 1107 90-91 0.9050537826278848 1107 92-93 0.7908684516718907 1107 94-95 1.0123519389129747 1107 96-97 1.4570272545099705 1107 98-99 1.4059359381653636 1107 100-101 1.002788996362142 1108 1 0.16420049926518132 1108 2 0.24056528630285356 1108 3 0.22394039471071636 1108 4 0.22229052010037265 1108 5 0.2593699647402232 1108 6 0.38990082565634054 1108 7 0.3331734805559847 1108 8 0.3120613663093792 1108 9 0.25546081508897345 1108 10-11 0.47798822211701975 1108 12-13 0.31406782649946763 1108 14-15 0.5514890041231482 1108 16-17 0.5815332367590074 1108 18-19 0.5823542698345321 1108 20-21 0.39236701743315194 1108 22-23 0.39400999396063696 1108 24-25 0.6070308991040463 1108 26-27 0.6640751520125292 1108 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2316 98-99 -3.117114388725934 2316 100-101 -2.760237711734705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8442.0 3 2951.0 4 3290.0 5 3691.0 6 4386.0 7 5405.0 8 6929.0 9 8709.0 10 10508.0 11 12910.0 12 15808.0 13 18442.0 14 20770.0 15 23918.0 16 27791.0 17 31549.0 18 30241.0 19 28257.0 20 29563.0 21 32154.0 22 34916.0 23 39528.0 24 43672.0 25 48573.0 26 54423.0 27 61014.0 28 69510.0 29 79303.0 30 91540.0 31 106020.0 32 124006.0 33 146743.0 34 176890.0 35 211509.0 36 237755.0 37 216302.0 38 90981.0 39 4360.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.88623703376942 18.234174297397114 14.368245803618453 23.511342865215013 2 15.710736927660626 19.86603082531473 39.48795115198031 24.935281095044335 3 19.346820199192344 23.716396244267063 29.654162986663113 27.28262056987748 4 13.041238187158461 16.039773082344446 36.072454409317416 34.846534321179675 5 13.91320898363152 37.50802308425419 33.54529094736764 15.033476984746647 6 32.93627230691407 35.36487328927681 16.898481007854365 14.800373395954756 7 29.274016273157432 32.74130045587596 19.824198470917036 18.160484800049577 8 27.539139551909887 33.69111723759006 19.414401727130574 19.355341483369475 9 27.265996171638957 16.18460673279122 19.518952884520665 37.03044421104916 10-11 25.544154559694164 26.070429689198605 26.703634451002035 21.681781300105197 12-13 26.64587200527384 23.42917613587079 27.020684789711385 22.904267069143984 14-15 23.098484133723307 24.79245637305776 25.489386716097894 26.61967277712104 16-17 22.596860735794245 27.94932146955343 26.483315518854596 22.970502275797735 18-19 22.83559805106924 26.160519443516804 28.832436172702575 22.17144633271138 20-21 23.24358259348094 25.440574668746375 29.012476616608613 22.30336612116408 22-23 22.94507353699559 25.44351371739353 29.021594193447232 22.589818552163653 24-25 22.7289275619764 25.666555643448934 29.115353123221894 22.48916367135277 26-27 22.066095304757834 26.06776446923415 28.878095246466618 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23.433821977254162 26.301432637726908 27.493081661252145 22.771663723766785 54-55 22.627107044228275 26.728779731681918 27.709261653598503 22.9348515704913 56-57 22.801869457519274 26.81219018005309 27.355949548786317 23.029990813641316 58-59 22.774179957994647 26.17891190738197 27.483861837862484 23.5630462967609 60-61 22.23403623087697 26.631491342333202 27.858658002101084 23.27581442468874 62-63 22.526152967548242 26.59518362279149 27.9879816001489 22.890681809511367 64-65 22.25056044480451 26.718327469933893 27.65702627616796 23.374085809093632 66-67 22.09936997536918 26.74708773432285 27.644691911273554 23.508850379034417 68-69 22.91176497789616 26.198347298522094 27.813176294323288 23.07671142925846 70-71 22.676152748581828 26.297537365033776 27.35807299796727 23.66823688841713 72-73 22.49135356185152 26.511958863396917 27.461494559612092 23.535193015139477 74-75 22.51162788546501 26.944897247024553 27.305024647902794 23.238450219607646 76-77 22.72375999012145 26.393529735771953 27.490830200424465 23.39188007368213 78-79 22.71680891129969 26.743979312393723 27.318876515937063 23.220335260369524 80-81 22.975048441715387 26.654006276095004 27.064798496104665 23.306146786084945 82-83 22.788835926198313 26.640551901188992 27.24835252945078 23.322259643161914 84-85 22.619167896830398 26.85641821790602 27.18911151930326 23.33530236596032 86-87 22.97731986413653 26.811462844485344 26.76209788366517 23.449119407712956 88-89 22.787540009990934 26.827554626357564 26.560782808192567 23.824122555458935 90-91 22.597144841401533 26.78009164129876 26.805183956736684 23.817579560563022 92-93 22.867323950306435 26.303679101775057 27.076134609790063 23.75286233812845 94-95 22.548806911328793 25.976161355621098 27.387611343223927 24.087420389826182 96-97 22.921505274381147 25.593318731743192 27.307880001757738 24.177295992117916 98-99 22.60588149597286 25.888710829916683 26.874602546937037 24.63080512717342 100-101 23.154275619290164 25.224622244103234 27.329186223847667 24.29191591275893 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 366.0 1 269.0 2 240.0 3 321.0 4 286.5 5 285.5 6 492.5 7 1061.0 8 1731.5 9 2019.0 10 1774.5 11 1457.0 12 1495.0 13 1645.5 14 1810.0 15 1990.5 16 2129.5 17 2365.0 18 2646.0 19 2915.0 20 3365.0 21 4063.0 22 4909.5 23 5923.5 24 7121.5 25 8807.5 26 11351.5 27 14488.0 28 17133.5 29 19769.5 30 22996.5 31 28112.0 32 35834.0 33 38343.0 34 37604.0 35 39623.5 36 43485.5 37 47784.5 38 51161.0 39 54408.0 40 57431.5 41 61137.5 42 63456.0 43 65448.0 44 68314.5 45 72578.0 46 75166.0 47 77196.0 48 79814.0 49 79269.5 50 77534.0 51 76016.5 52 75424.5 53 76597.5 54 78691.0 55 76682.0 56 71511.5 57 66412.5 58 63061.0 59 56559.0 60 45794.5 61 38392.5 62 33240.5 63 25701.0 64 18336.5 65 14067.5 66 10814.0 67 8396.5 68 6537.5 69 5099.5 70 3991.0 71 2756.0 72 2003.5 73 1664.5 74 1265.0 75 918.0 76 673.5 77 460.0 78 314.0 79 200.5 80 137.0 81 96.0 82 64.0 83 39.5 84 25.0 85 15.5 86 9.0 87 12.5 88 10.0 89 4.5 90 3.0 91 2.0 92 2.0 93 2.5 94 2.5 95 1.5 96 1.5 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.005640942888227491 2 0.10458862961615233 3 0.11258767158060608 4 0.11577804091902981 5 0.15558830179414349 6 0.09478633541693735 7 0.02533800576023496 8 0.025892852601699955 9 0.022008924711444965 10-11 0.04887275928570867 12-13 0.053010991978301794 14-15 0.02603156431206621 16-17 0.03051657628057495 18-19 0.034816639301928694 20-21 0.057126106052500535 22-23 0.030054203912687455 24-25 0.04850286139139867 26-27 0.05541532829131679 28-29 0.051878179676977415 30-31 0.06572623209520802 32-33 0.04878028481213117 34-35 0.059923458878219905 36-37 0.051115265269963045 38-39 0.031510676871533076 40-41 0.036041926076830565 42-43 0.0323198285153362 44-45 0.007120534465467489 46-47 0.012784595972089354 48-49 0.008646363279496236 50-51 0.008345821240369361 52-53 0.00968670110724311 54-55 0.011605546433976231 56-57 0.014495373733273103 58-59 0.019211571885725594 60-61 0.025083700957896832 62-63 0.011166292684483107 64-65 0.008831312226651237 66-67 0.010380259659074359 68-69 0.011050699592511232 70-71 0.02977678049195495 72-73 0.027603630362883703 74-75 0.02808912134916558 76-77 0.02302614392079746 78-79 0.005155451901945616 80-81 0.005016740191579367 82-83 0.005802773216988116 84-85 0.004924265718001867 86-87 0.013593747615892477 88-89 0.03694355219421119 90-91 0.03433114831564682 92-93 0.029036984703334955 94-95 0.045081305869031175 96-97 0.04133608968914244 98-99 0.02561542918096746 100-101 0.07282364794228113 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2162759.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.98598224423116 #Duplication Level Percentage of deduplicated Percentage of total 1 74.33277463830203 37.155967532346004 2 13.491616922946248 13.487834479127194 3 4.990705026457879 7.483958785161561 4 2.367004345688456 4.732681487824046 5 1.3114761147240577 3.2777710892165004 6 0.7810341878747604 2.3424456628347166 7 0.5291300354195897 1.851435918876111 8 0.36114241723710844 1.4441646764522258 9 0.28091414492635275 1.263759251439784 >10 1.395690170595031 12.689981590684402 >50 0.09628059335777993 3.3336395529875116 >100 0.054153557828608756 5.144823199438605 >500 0.0038996491376209154 1.385147611597613 >1k 0.003992497926611884 3.820259854362009 >5k 1.8569757798194808E-4 0.5861293076517418 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7157 0.33091990369708324 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5472 0.2530101597080396 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3957 0.18296074597308346 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3842 0.17764346374237722 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3821 0.17667248176981346 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3652 0.16885838875251474 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3233 0.14948498653802852 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2901 0.13413422392416352 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2890 0.1336256143194873 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2705 0.12507172551356854 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2702 0.12493301380320229 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2590 0.1197544432828623 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2419 0.11184787579198607 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2215 0.10241547948708109 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 9.247447357749986E-5 2 9.247447357749986E-5 0.0 0.0 0.0 9.247447357749986E-5 3 9.247447357749986E-5 0.0 0.0 4.623723678874993E-5 9.247447357749986E-5 4 9.247447357749986E-5 0.0 0.0 4.623723678874993E-5 9.247447357749986E-5 5 9.247447357749986E-5 0.0 0.0 4.623723678874993E-5 9.247447357749986E-5 6 1.849489471549997E-4 0.0 0.0 4.623723678874993E-5 1.3871171036624979E-4 7 2.3118618394374961E-4 0.0 0.0 4.623723678874993E-5 1.3871171036624979E-4 8 2.3118618394374961E-4 0.0 0.0 4.623723678874993E-5 1.3871171036624979E-4 9 2.3118618394374961E-4 0.0 0.0 4.623723678874993E-5 1.3871171036624979E-4 10-11 2.3118618394374961E-4 0.0 0.0 9.247447357749986E-5 1.3871171036624979E-4 12-13 2.7742342073249957E-4 0.0 0.0 9.247447357749986E-5 3.930165127043744E-4 14-15 3.236606575212495E-4 0.0 0.0 9.247447357749986E-5 4.8549098628187425E-4 16-17 3.4677927591562446E-4 0.0 0.0 1.1559309197187482E-4 5.548468414649991E-4 18-19 4.392537494931243E-4 0.0 0.0 1.849489471549997E-4 6.010840782537491E-4 20-21 5.548468414649991E-4 0.0 0.0 3.4677927591562446E-4 7.397957886199989E-4 22-23 6.010840782537491E-4 0.0 0.0 5.317282230706241E-4 7.860330254087488E-4 24-25 6.935585518312489E-4 0.0 0.0 9.247447357749985E-4 7.860330254087488E-4 26-27 7.397957886199989E-4 0.0 0.0 0.0019882011819162466 7.860330254087488E-4 28-29 7.397957886199989E-4 0.0 0.0 0.004970502954790617 7.860330254087488E-4 30-31 7.860330254087488E-4 0.0 0.0 0.015951846692118722 7.860330254087488E-4 32-33 7.860330254087488E-4 0.0 0.0 0.03798389002195807 8.091516438031238E-4 34-35 9.016261173806235E-4 0.0 0.0 0.07245375004797114 8.322702621974987E-4 36-37 9.709819725637484E-4 0.0 0.0 0.1292793140613448 8.322702621974987E-4 38-39 0.0010634564461412482 0.0 0.0 0.21437894837103905 8.785074989862485E-4 40-41 0.0010865750645356232 0.0 0.0 0.30498081385859455 8.785074989862485E-4 42-43 0.001155930919718748 0.0 0.0 0.40365107716578685 9.016261173806234E-4 44-45 0.001248405393296248 0.0 0.0 0.5276131089964254 9.478633541693735E-4 46-47 0.001363998485268123 0.0 0.0 0.6717345760669589 9.709819725637484E-4 48-49 0.0014333543404512477 0.0 0.0 0.8193700731334375 9.709819725637484E-4 50-51 0.0014333543404512477 0.0 0.0 0.985685413862571 0.0012021681565074981 52-53 0.0014333543404512477 0.0 0.0 1.145943676572378 0.001248405393296248 54-55 0.0014795915772399977 0.0 0.0 1.3172295202563022 0.001294642630084998 56-57 0.0014795915772399977 0.0 0.0 1.5184770933793363 0.001317761248479373 58-59 0.0014795915772399977 0.0 0.0 1.7378496633235603 0.0013408798668737478 60-61 0.0014795915772399977 0.0 0.0 1.9806645123196804 0.0013408798668737478 62-63 0.0015258288140287475 0.0 0.0 2.2197803823727007 0.0013871171036624979 64-65 0.0015258288140287475 0.0 0.0 2.465693126233667 0.0013871171036624979 66-67 0.0016183032876062474 0.0 0.0 2.7698879070668534 0.0013871171036624979 68-69 0.0016645405243949974 0.0 0.0 3.1005997431983867 0.0013871171036624979 70-71 0.0016645405243949974 0.0 0.0 3.4368138105077817 0.0013871171036624979 72-73 0.0016876591427893723 0.0 0.0 3.7914302980590993 0.0015027101956343726 74-75 0.0017107777611837472 0.0 0.0 4.1407526219981055 0.0016645405243949974 76-77 0.0017107777611837472 0.0 0.0 4.520753352546446 0.0017107777611837472 78-79 0.0017107777611837472 0.0 0.0 4.951661280799201 0.0017338963795781222 80-81 0.002034438418704997 0.0 0.0 5.397503836534723 0.0017801336163668722 82-83 0.0020806756554937467 0.0 0.0 5.861586982183406 0.001849489471549997 84-85 0.0020806756554937467 0.0 0.0 6.329970190853443 0.001895726708338747 86-87 0.0020806756554937467 0.0 0.0 6.815253109569767 0.0019419639451274968 88-89 0.0020806756554937467 0.0 0.0 7.31949329536948 0.0019882011819162466 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4810 0.0 43.204803 1 GTATCAA 8340 0.0 39.62912 1 TATCAAC 10780 0.0 30.705511 2 ATCAACG 10775 0.0 30.631487 3 AGGTAAG 1410 0.0 30.356329 2 TCAACGC 10860 0.0 30.304152 4 CAACGCA 11015 0.0 29.705019 5 AACGCAG 11365 0.0 28.891663 6 TAAGGTG 1605 0.0 28.446054 5 GTAAGGT 1635 0.0 27.633232 4 GGTAAGG 1640 0.0 26.969007 3 TACATGG 9480 0.0 26.086803 2 GTACATG 9740 0.0 25.87692 1 ACGCAGA 13005 0.0 25.030193 7 CGCAGAG 13080 0.0 24.814009 8 AAGGTAA 1615 0.0 24.440002 1 ACATGGG 9890 0.0 24.4283 3 GTGTAAG 845 0.0 24.199556 1 TAAATCG 100 0.0016423579 23.779123 5 GGACCGA 730 0.0 22.131964 6 >>END_MODULE