FastQCFastQC Report
Thu 26 May 2016
SRR938463_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938463_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2162759
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71050.32851556738406823No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58920.2724297991593146No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40280.1862435897850847No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40150.18564250570683097No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39630.18323816939381596No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35120.16238517560208973No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34440.15924104350045473No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA31430.14532363522704103No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31010.1433816712819135No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA27140.1254878606446673No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25770.11915335920460857No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24720.11429844934178982No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24240.11207906197592982No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT22950.10611445843018107No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA22540.10421873172184234No Hit
CCTTAATACTTCATTTTTGTTTTTAATTTCTGAATGGCCCAGGTCTGAGG22290.10306280080212359No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.049.683041
GTATCAA80950.039.2122421
AGGTAAG12400.029.5067272
TATCAAC108650.028.9521122
TCAACGC109050.028.7936824
TAAGGTG14900.028.6931115
ATCAACG109400.028.658143
CAACGCA111850.028.0728765
AACGCAG115900.027.2029046
GTAAGGT15200.026.8767224
AAGGTAA14450.024.005851
GGTAAGG15650.023.9791723
TACATGG93050.023.6948322
ACGCAGA131750.023.6780197
CGCAGAG132200.023.5255858
TAAGGTA7150.023.2532374
GTACCGT2252.8285285E-923.2135096
GTACATG96600.023.1689221
ACATGGG96700.021.958493
GCAGAGT144700.021.1323599
Produced by FastQC (version 0.11.5)