FastQCFastQC Report
Thu 26 May 2016
SRR938462_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938462_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173740
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71230.3276840836530588No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56580.26028871898203093No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40060.18429066953729517No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39600.1821745010902868No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37270.17145564786957043No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37120.1707655929411981No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33000.15181208424190565No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29260.13460671469448968No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG29140.13405467075179184No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA28010.12885625695805386No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA27470.12637205921591357No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25400.11684930120437587No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25150.1156992096570887No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT22320.10268017334179802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47550.049.863221
GTATCAA84350.040.4702221
TATCAAC107900.031.5400522
TCAACGC109050.031.2510284
ATCAACG108950.031.2360883
CAACGCA110950.030.7137375
TAAGGTG15650.030.0650335
AACGCAG114750.029.6911686
AAGGTAA14700.026.5581651
GTAAGGT16350.026.1634834
ACGCAGA130650.025.7063757
AGGTAAG15050.025.5811082
CGCAGAG131500.025.4679628
TACATGG93900.024.6509782
GGTAAGG16600.024.6241463
TAAGGTA7550.024.5520924
GTACATG97600.024.2929951
GTGTAAG9950.023.4463121
ACATGGG99100.022.5421353
GCAGAGT141550.022.5161199
Produced by FastQC (version 0.11.5)