##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938462_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173740 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.93988011445711 31.0 31.0 34.0 27.0 34.0 2 31.037075271191586 31.0 31.0 34.0 27.0 34.0 3 31.08780396919595 31.0 31.0 34.0 27.0 34.0 4 34.729102836585795 37.0 35.0 37.0 32.0 37.0 5 34.74421963988333 37.0 35.0 37.0 32.0 37.0 6 34.77612271936846 37.0 35.0 37.0 32.0 37.0 7 34.694478180463165 37.0 35.0 37.0 30.0 37.0 8 34.644449198156174 37.0 35.0 37.0 30.0 37.0 9 36.129198984239146 39.0 35.0 39.0 31.0 39.0 10-11 35.97478953324685 38.0 35.0 39.0 30.0 39.0 12-13 35.82327969306357 38.0 35.0 39.0 30.0 39.0 14-15 36.78252918932347 39.0 36.0 41.0 30.0 41.0 16-17 36.64524069115902 39.0 36.0 41.0 30.0 41.0 18-19 36.639669647703954 39.0 36.0 40.5 30.0 41.0 20-21 36.70490744063227 39.0 36.0 41.0 30.0 41.0 22-23 36.62306968634704 39.0 36.0 41.0 30.0 41.0 24-25 36.494305436712764 39.0 36.0 41.0 29.5 41.0 26-27 36.294476570335 39.0 35.0 41.0 29.0 41.0 28-29 36.1087363254115 39.0 35.0 40.0 28.5 41.0 30-31 35.86499512361184 38.5 35.0 40.0 27.0 41.0 32-33 35.56544135913219 38.0 34.5 40.0 27.0 41.0 34-35 35.3051901331346 38.0 34.0 40.0 26.0 41.0 36-37 34.99812880105256 38.0 34.0 40.0 25.0 41.0 38-39 34.714999493959716 38.0 33.5 40.0 24.5 41.0 40-41 34.636366814798464 38.0 33.0 40.0 24.0 41.0 42-43 34.43159876526171 38.0 33.0 40.0 24.0 41.0 44-45 34.12426256129988 38.0 33.0 40.0 23.0 41.0 46-47 33.73058300440715 38.0 33.0 40.0 21.5 41.0 48-49 33.3304164711511 37.5 32.0 40.0 18.5 41.0 50-51 32.56274577456365 36.5 31.0 39.5 17.0 40.5 52-53 32.61952073385041 37.0 31.0 39.0 17.0 40.0 54-55 32.74443401694775 37.0 31.5 40.0 14.5 41.0 56-57 32.43415794897274 36.5 31.0 40.0 12.5 41.0 58-59 32.097171694866915 36.0 31.0 39.0 8.5 41.0 60-61 31.646712348302927 35.5 30.5 39.0 7.5 40.5 62-63 31.12588718061958 35.0 29.5 39.0 6.0 40.0 64-65 30.62722151683274 35.0 29.0 38.0 2.0 40.0 66-67 30.09578238427779 34.5 28.5 38.0 2.0 40.0 68-69 29.540166717270694 34.0 28.0 37.0 2.0 39.5 70-71 28.91293024924784 34.0 26.5 36.5 2.0 39.0 72-73 28.39169104860747 34.0 26.0 36.0 2.0 39.0 74-75 27.814745783764387 33.0 26.0 35.5 2.0 38.5 76-77 27.116464250554344 33.0 24.5 35.0 2.0 37.5 78-79 26.368621362260434 32.0 22.5 35.0 2.0 37.0 80-81 25.754183342994104 32.0 20.5 35.0 2.0 36.0 82-83 25.12128244408255 31.0 18.5 34.5 2.0 36.0 84-85 24.5015979371958 31.0 16.5 34.0 2.0 35.0 86-87 23.875730078114216 31.0 8.5 34.0 2.0 35.0 88-89 23.095507742416295 30.0 4.0 34.0 2.0 35.0 90-91 22.286438120474386 29.0 2.0 34.0 2.0 35.0 92-93 21.47307543680477 29.0 2.0 34.0 2.0 35.0 94-95 20.543083119416305 28.0 2.0 34.0 2.0 35.0 96-97 19.348284983484685 26.0 2.0 33.5 2.0 35.0 98-99 17.914689659296883 24.0 2.0 33.0 2.0 34.5 100-101 14.666769944887614 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8984378887729925 1101 2 0.8307232708039223 1101 3 0.9252665581942949 1101 4 0.7697713695880637 1101 5 0.7115170216130835 1101 6 0.7177158267399335 1101 7 0.7454671159811781 1101 8 0.7440693010157986 1101 9 0.805090335251009 1101 10-11 0.850758229006594 1101 12-13 0.7717502241556247 1101 14-15 1.012819129380894 1101 16-17 1.0762927716988813 1101 18-19 1.0465967035758084 1101 20-21 1.0583600039624415 1101 22-23 1.059376554127553 1101 24-25 1.242207690957727 1101 26-27 1.1565745577580273 1101 28-29 1.3370231003022894 1101 30-31 1.095394443757364 1101 32-33 1.070788137876825 1101 34-35 1.317047214729854 1101 36-37 1.2132382416442837 1101 38-39 1.2873266260266831 1101 40-41 1.186252371629017 1101 42-43 1.1847977258937519 1101 44-45 1.1496337570086368 1101 46-47 1.1451523799139451 1101 48-49 1.2805405887006671 1101 50-51 1.1318259025461828 1101 52-53 1.475284805339193 1101 54-55 1.422640456985583 1101 56-57 1.429128174908449 1101 58-59 1.5832401922036397 1101 60-61 1.5393549295198596 1101 62-63 1.2505460181645098 1101 64-65 1.3268191977176862 1101 66-67 1.5018778753472155 1101 68-69 1.6605997214992314 1101 70-71 1.708631578789106 1101 72-73 1.603961784455894 1101 74-75 1.6064478752988265 1101 76-77 1.8959240032906841 1101 78-79 1.601188486800023 1101 80-81 1.5524270674151133 1101 82-83 1.4708946174452358 1101 84-85 1.802200702074348 1101 86-87 1.7826193637676013 1101 88-89 1.8059711286860072 1101 90-91 1.8913223460821165 1101 92-93 2.0416748869382175 1101 94-95 2.0202113363487015 1101 96-97 1.842037346807352 1101 98-99 2.012838640164002 1101 100-101 1.6495120341700744 1102 1 0.8226072801487376 1102 2 0.8104254100060082 1102 3 0.7620424331558056 1102 4 0.8143208866809459 1102 5 0.6971528650301124 1102 6 0.6793123526573055 1102 7 0.6619268548699111 1102 8 0.6348381402583101 1102 9 0.6960856517536342 1102 10-11 0.8717416744352633 1102 12-13 0.8168333373827323 1102 14-15 0.8854819626035706 1102 16-17 0.9926020930316284 1102 18-19 0.9466145861155368 1102 20-21 1.0154892986520352 1102 22-23 1.0254532715321005 1102 24-25 0.9599632201447506 1102 26-27 0.8553269429694055 1102 28-29 0.9125585030181327 1102 30-31 0.8702894002004271 1102 32-33 0.8646861073838252 1102 34-35 0.8932898025087752 1102 36-37 0.9992383076237914 1102 38-39 1.0447082137658086 1102 40-41 1.0570122722199002 1102 42-43 1.1694866849047756 1102 44-45 1.182772092984635 1102 46-47 1.0117534772682788 1102 48-49 0.8406479153578559 1102 50-51 1.0489635022747308 1102 52-53 1.0826060951442074 1102 54-55 1.1247593677582515 1102 56-57 1.0604641708114357 1102 58-59 1.116547535198606 1102 60-61 1.079755461754516 1102 62-63 0.9402145706580107 1102 64-65 0.8986779450287585 1102 66-67 0.9329223570520462 1102 68-69 0.817175233468749 1102 70-71 0.8166008815638044 1102 72-73 0.859278239953241 1102 74-75 0.8341166938049263 1102 76-77 0.9327993449702454 1102 78-79 0.921084082092225 1102 80-81 0.8779783104479897 1102 82-83 1.0252280830866738 1102 84-85 1.002850652537358 1102 86-87 0.9052044354339088 1102 88-89 1.1029105699757693 1102 90-91 1.16141271181802 1102 92-93 1.3332161838229375 1102 94-95 1.3920794045736002 1102 96-97 1.414021507537612 1102 98-99 1.1869497758283316 1102 100-101 0.8880754561812569 1103 1 0.6715196989928103 1103 2 0.7482569550122875 1103 3 0.6749381256252711 1103 4 0.7337142935968188 1103 5 0.6518889477816501 1103 6 0.6966902454065931 1103 7 0.6802654312067062 1103 8 0.6361429232398876 1103 9 0.6541736468768562 1103 10-11 0.6658654349350925 1103 12-13 0.7269159736382349 1103 14-15 0.8971333078225925 1103 16-17 0.9864144173793719 1103 18-19 1.0995711335235328 1103 20-21 0.8380161060908691 1103 22-23 0.8334353294560799 1103 24-25 0.9861383545094569 1103 26-27 0.9609768104823999 1103 28-29 0.9314036216101513 1103 30-31 1.0807175332079382 1103 32-33 1.0970580002274275 1103 34-35 1.0365836197238707 1103 36-37 1.112307272478482 1103 38-39 0.8963131667010487 1103 40-41 1.243883570313038 1103 42-43 1.1967875657642892 1103 44-45 1.2455213248744457 1103 46-47 1.2686752025162633 1103 48-49 1.173068017050575 1103 50-51 1.4129534133503654 1103 52-53 1.3882510719043992 1103 54-55 1.2930763723817549 1103 56-57 1.3795360107301065 1103 58-59 1.258650104765195 1103 60-61 1.4267272997518177 1103 62-63 1.443866914987968 1103 64-65 1.4672183522537985 1103 66-67 1.5040408896864683 1103 68-69 1.5773143753861696 1103 70-71 1.6432167076905557 1103 72-73 1.6101013711179881 1103 74-75 1.562063328704987 1103 76-77 1.445511808879111 1103 78-79 1.410529662860391 1103 80-81 1.409417117523514 1103 82-83 1.3263613403804442 1103 84-85 1.5761150029667839 1103 86-87 1.552668492767502 1103 88-89 1.5665827567885522 1103 90-91 1.4269098481075773 1103 92-93 1.5459848913891072 1103 94-95 1.5990892507070242 1103 96-97 1.6335813747187373 1103 98-99 1.680932403175678 1103 100-101 1.0847701806310042 1104 1 0.6230635729050924 1104 2 0.6197713116398198 1104 3 0.6572537216766001 1104 4 0.6953803016484486 1104 5 0.6314175871490804 1104 6 0.626116929277515 1104 7 0.6897294224327268 1104 8 0.5883933329267705 1104 9 0.8597500625096828 1104 10-11 0.6304956827868082 1104 12-13 0.6118540378576824 1104 14-15 0.7499357974346523 1104 16-17 0.6318662438203759 1104 18-19 0.7697795674786008 1104 20-21 0.8772463102237609 1104 22-23 0.8761933731758447 1104 24-25 0.9675598629409095 1104 26-27 0.9654483108641116 1104 28-29 0.9505010971080239 1104 30-31 0.9582920924434006 1104 32-33 0.8721448595118417 1104 34-35 0.9104684003342456 1104 36-37 0.8713042135775311 1104 38-39 0.8379330811424666 1104 40-41 0.9780916813342841 1104 42-43 0.9787825650112509 1104 44-45 0.9349314560261774 1104 46-47 1.0242841712006552 1104 48-49 1.2006947933636667 1104 50-51 1.0489433731299869 1104 52-53 1.105194852619114 1104 54-55 1.2492204008679195 1104 56-57 1.2124517490800528 1104 58-59 1.1980559867876508 1104 60-61 1.215789427215313 1104 62-63 1.2542515272856072 1104 64-65 1.2290011877544238 1104 66-67 1.1409387467561452 1104 68-69 1.3121443936904775 1104 70-71 1.3592800924241892 1104 72-73 1.370255373726959 1104 74-75 1.2713167755750554 1104 76-77 1.3714228103941615 1104 78-79 1.4480860641500506 1104 80-81 1.5657015089136443 1104 82-83 1.3938807128690307 1104 84-85 1.6490581529218602 1104 86-87 1.456617043365899 1104 88-89 1.674583583952387 1104 90-91 1.771840235566792 1104 92-93 1.6946878918881438 1104 94-95 1.2510670315378825 1104 96-97 0.9735244647138792 1104 98-99 1.1919690443617306 1104 100-101 1.0325212345330979 1105 1 0.5671399293573174 1105 2 0.6337211487477994 1105 3 0.5957420313938613 1105 4 0.5641900777864066 1105 5 0.592413779793425 1105 6 0.5456364959454874 1105 7 0.5372114650594071 1105 8 0.5734190423902064 1105 9 0.5878263666271195 1105 10-11 0.6253629486867638 1105 12-13 0.7173531154570867 1105 14-15 0.7635834011556568 1105 16-17 0.8535969551809544 1105 18-19 0.7807535178390594 1105 20-21 0.7979538765253977 1105 22-23 0.9546386375319287 1105 24-25 0.936322537771801 1105 26-27 0.9831047591638864 1105 28-29 0.8201672011757424 1105 30-31 0.892538667785864 1105 32-33 0.9790203419731469 1105 34-35 0.9664103610569867 1105 36-37 1.058472378327437 1105 38-39 1.1213122074132258 1105 40-41 0.9578819496809032 1105 42-43 0.9011131638520311 1105 44-45 0.8106402652789697 1105 46-47 0.9841420822576836 1105 48-49 1.0923009479344543 1105 50-51 1.11353169462258 1105 52-53 1.0396003948729131 1105 54-55 1.1007498849777733 1105 56-57 1.2227636644023718 1105 58-59 1.3300717918929124 1105 60-61 1.3936348040911852 1105 62-63 1.3690060117225507 1105 64-65 1.2776012322389896 1105 66-67 1.3564102177561317 1105 68-69 1.292383432823808 1105 70-71 1.4565547584628398 1105 72-73 1.585700023149311 1105 74-75 1.2082625174860588 1105 76-77 1.4299174002856994 1105 78-79 1.3092556157717716 1105 80-81 1.2595672178537356 1105 82-83 1.3940337921169785 1105 84-85 1.3298584826310105 1105 86-87 1.5220813183328623 1105 88-89 1.5336492771920405 1105 90-91 1.5255847894781631 1105 92-93 1.5489924280056897 1105 94-95 1.609400691963625 1105 96-97 1.6497298002452752 1105 98-99 1.5487437823607095 1105 100-101 1.3737832878884095 1106 1 0.34002249254018224 1106 2 0.39198293631092085 1106 3 0.0776603483829632 1106 4 0.3588823355801196 1106 5 0.46149583166251773 1106 6 0.03690727601696864 1106 7 0.27033111502569795 1106 8 0.46867603479681463 1106 9 0.4479833221479126 1106 10-11 0.10141006078149672 1106 12-13 0.14836836583548774 1106 14-15 0.5813216644695345 1106 16-17 0.2995166204103441 1106 18-19 0.10686549809879153 1106 20-21 0.36794662655401567 1106 22-23 0.3995496663919056 1106 24-25 0.4416169247676365 1106 26-27 0.16567684550214778 1106 28-29 0.35372439327454686 1106 30-31 0.46107760776373397 1106 32-33 0.4854391104955553 1106 34-35 0.4324951221259141 1106 36-37 0.277698793661763 1106 38-39 0.26490043447724076 1106 40-41 0.5394428558001252 1106 42-43 0.07285290259867594 1106 44-45 0.4411995306152079 1106 46-47 -0.05060881126949113 1106 48-49 0.4279224739898382 1106 50-51 0.28186712663183755 1106 52-53 0.469414948236512 1106 54-55 0.35180558256493555 1106 56-57 -0.036421540568312594 1106 58-59 -0.4084567183015686 1106 60-61 0.2878882478275955 1106 62-63 0.3810250651561127 1106 64-65 0.6091468752605742 1106 66-67 0.06575377736488619 1106 68-69 -0.1493738584851272 1106 70-71 -0.19891642608073212 1106 72-73 0.41694867172920524 1106 74-75 0.3167297209049913 1106 76-77 0.33013426346474617 1106 78-79 0.4145035775122743 1106 80-81 0.5780813552979289 1106 82-83 0.5496766865090663 1106 84-85 0.362629625201059 1106 86-87 0.7301682162354588 1106 88-89 0.8341860294592465 1106 90-91 0.6731287026820141 1106 92-93 0.5366757441680363 1106 94-95 0.17897752258139832 1106 96-97 0.2442967734458179 1106 98-99 0.24235108829678254 1106 100-101 -0.03857791331737381 1107 1 0.5150383941040175 1107 2 0.4426498271835442 1107 3 0.40597585490564825 1107 4 0.548567886724669 1107 5 0.5365633980774405 1107 6 0.48547637897380724 1107 7 0.4158761182791224 1107 8 0.478775050971322 1107 9 0.4087326574503578 1107 10-11 0.5364530863921573 1107 12-13 0.5751462337830517 1107 14-15 0.6011066217119918 1107 16-17 0.6387256120440483 1107 18-19 0.8380498660695039 1107 20-21 0.8104042894018662 1107 22-23 0.764903022292053 1107 24-25 0.8248129319519748 1107 26-27 0.649540718367625 1107 28-29 0.7972366770802992 1107 30-31 0.9123603339132202 1107 32-33 0.9839622756013071 1107 34-35 0.9421944668108893 1107 36-37 0.8701216071053466 1107 38-39 0.978659491057563 1107 40-41 0.8151351526195683 1107 42-43 0.9592322688269235 1107 44-45 0.9651609330722124 1107 46-47 1.0302673953606742 1107 48-49 0.8674642408790021 1107 50-51 0.8118028867509608 1107 52-53 0.5971879738228054 1107 54-55 0.7834282488124344 1107 56-57 0.8730668799250907 1107 58-59 0.8414196028685978 1107 60-61 0.9504625465137977 1107 62-63 1.0727662437483438 1107 64-65 0.9660388932847859 1107 66-67 0.892148301865479 1107 68-69 0.9094527888803334 1107 70-71 1.0320985689111914 1107 72-73 0.7062779303629618 1107 74-75 1.0555785207938655 1107 76-77 1.1118892599676222 1107 78-79 0.9075885296369925 1107 80-81 0.880938519043827 1107 82-83 0.8475860878060466 1107 84-85 0.797748198471325 1107 86-87 0.8812598799167759 1107 88-89 0.958795192171845 1107 90-91 1.252111953034774 1107 92-93 1.0907307038718912 1107 94-95 0.9529486731235153 1107 96-97 1.0447315380417486 1107 98-99 1.2473817094691348 1107 100-101 0.6163916604719741 1108 1 0.21356492830910412 1108 2 0.2605405947728805 1108 3 0.2929430065302796 1108 4 0.28084446540113106 1108 5 0.31036954725413324 1108 6 0.2352452234563529 1108 7 0.09356193116423128 1108 8 0.12847411843048206 1108 9 0.1168372740191117 1108 10-11 0.1942871639754884 1108 12-13 0.2312849477511918 1108 14-15 0.27473827094779324 1108 16-17 0.18996600957415666 1108 18-19 0.12248352644437688 1108 20-21 0.12564972740379687 1108 22-23 0.1855777553968494 1108 24-25 0.14473848017406254 1108 26-27 0.2542721475992167 1108 28-29 0.07842191862776815 1108 30-31 0.2891476528573307 1108 32-33 0.2287941278943677 1108 34-35 0.3113392124365362 1108 36-37 0.25101320367938484 1108 38-39 0.24201209340771612 1108 40-41 0.1122669688382203 1108 42-43 0.1650025685276546 1108 44-45 0.1776203971739534 1108 46-47 0.3250700591291391 1108 48-49 0.3688994708543518 1108 50-51 0.34398074853660887 1108 52-53 0.39616779089529786 1108 54-55 0.36211150205902953 1108 56-57 0.39172152229679824 1108 58-59 0.36504203266215995 1108 60-61 0.3246880272924102 1108 62-63 0.5295907541519647 1108 64-65 0.43603314739174337 1108 66-67 0.3709267525477742 1108 68-69 0.3088883753917919 1108 70-71 0.3189392305534504 1108 72-73 0.17102006715424167 1108 74-75 0.2978519778666495 1108 76-77 0.37258840355412204 1108 78-79 0.3530016849777091 1108 80-81 0.4241483424551653 1108 82-83 0.502136169461231 1108 84-85 0.4628670699809909 1108 86-87 0.5254061461765609 1108 88-89 0.6524421166079968 1108 90-91 0.6339991346053786 1108 92-93 0.7948618747295768 1108 94-95 0.8092714125143665 1108 96-97 0.9203398395182774 1108 98-99 0.7275937642915373 1108 100-101 0.5937150842438275 1109 1 0.501211231845943 1109 2 0.5568389337630073 1109 3 0.33587702878341474 1109 4 0.4193682925723081 1109 5 0.3981957121869968 1109 6 0.44048977226334785 1109 7 0.40175123915217625 1109 8 0.5002519489362101 1109 9 0.4801069026388589 1109 10-11 0.3553024940546763 1109 12-13 0.3808097613230501 1109 14-15 0.3548100210828906 1109 16-17 0.5564585020571187 1109 18-19 0.46872951001600427 1109 20-21 0.3915122883793103 1109 22-23 0.4929813353250694 1109 24-25 0.4543874508812138 1109 26-27 0.3640580280233294 1109 28-29 0.3247806478770485 1109 30-31 0.2955576701808482 1109 32-33 0.36472607418274805 1109 34-35 0.35272499033211346 1109 36-37 0.5751467105917314 1109 38-39 0.6496817555158856 1109 40-41 0.6189835996489919 1109 42-43 0.7210619493727535 1109 44-45 0.5600460778737499 1109 46-47 0.6275801235110308 1109 48-49 0.6893088787203254 1109 50-51 0.7929992877906642 1109 52-53 0.7499189108132143 1109 54-55 0.8068923076381083 1109 56-57 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-1.7989495841198782 2316 96-97 -1.8127650613796753 2316 98-99 -1.6534315556610437 2316 100-101 -1.420728927556711 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7773.0 3 2843.0 4 3115.0 5 3355.0 6 4060.0 7 5086.0 8 6349.0 9 7950.0 10 9857.0 11 12303.0 12 14770.0 13 17115.0 14 19530.0 15 23159.0 16 26178.0 17 29732.0 18 27805.0 19 26231.0 20 27505.0 21 29178.0 22 32630.0 23 36384.0 24 40168.0 25 45070.0 26 50840.0 27 57882.0 28 66280.0 29 75840.0 30 87364.0 31 102204.0 32 120834.0 33 145365.0 34 177098.0 35 217429.0 36 252495.0 37 242020.0 38 113152.0 39 6791.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9794562089479 18.238467621960325 14.325717898943868 23.456358270147902 2 15.698335618446407 19.847208088886063 39.52256094670417 24.93189534596336 3 19.362931448695598 23.733567256198924 29.64171509888641 27.261786196219067 4 13.140773088450805 16.05702245329288 35.99891010582284 34.80329435243348 5 13.921191544519054 37.44418892818807 33.58942751879395 15.045192008498933 6 33.010394321330914 35.34571015586879 16.89081819604666 14.753077326753639 7 29.299229393938695 32.75892616770174 19.793001728053603 18.148842710305964 8 27.530021178426168 33.72160447977557 19.362106908253512 19.38626743354475 9 27.21749243282275 16.18112626106198 19.555049517435688 37.04633178867959 10-11 25.52877605796632 26.06323590053239 26.732403739239928 21.675584302261363 12-13 26.64363157911692 23.474665622294825 27.006139673075406 22.875563125512848 14-15 23.12874204995942 24.808498389455984 25.47349349880478 26.58926606177982 16-17 22.56872581622297 27.981750272137596 26.4919939431409 22.957529968498534 18-19 22.838346405326515 26.182974155536197 28.80575635584201 22.17292308329527 20-21 23.244763857817833 25.415601633663016 29.05350966682139 22.28612484169776 22-23 22.95181184525463 25.4450651906219 28.994747884414334 22.608375079709134 24-25 22.738991561706847 25.70244112287463 29.060863844971653 22.49770347044687 26-27 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26.792365761776725 27.40802494014329 22.699749347143165 52-53 23.395928316395015 26.324859151511358 27.508007159802965 22.771205372290662 54-55 22.660548411533405 26.643234366024682 27.732631606552673 22.963585615889244 56-57 22.772702229879954 26.855658779023695 27.343639833037926 23.027999158058428 58-59 22.707280832778736 26.174761426817504 27.540350343651664 23.577607396752096 60-61 22.21412728069929 26.617440629470014 27.907197157042223 23.26123493278847 62-63 22.518532252076355 26.60121740322583 28.012810751351537 22.867439593346287 64-65 22.213350521582388 26.694464150814923 27.700202155156152 23.391983172446537 66-67 22.085950394212468 26.68609646272665 27.70760993955984 23.520343203501042 68-69 22.901030273766608 26.167854087451925 27.80630383909078 23.12481179969069 70-71 22.646097341400754 26.288284213386426 27.359228737728447 23.70638970748437 72-73 22.538352418476524 26.497689491384424 27.456816287992947 23.507141802146105 74-75 22.508252374024188 26.962049662404976 27.333095907908383 23.196602055662456 76-77 22.684820946297233 26.42920438264499 27.536367911029398 23.34960676002838 78-79 22.720457557805457 26.716290329481552 27.381353838538075 23.181898274174916 80-81 22.937891706433962 26.639108476443845 27.103963157598116 23.31903665952408 82-83 22.765051268337846 26.668138870198803 27.309628556147885 23.25718130531546 84-85 22.584402099177726 26.888664173429216 27.264237009992122 23.262696717400935 86-87 22.986555721386626 26.845310283019042 26.812875075222074 23.355258920372254 88-89 22.731882740832944 26.830201529127272 26.66932795247419 23.768587777565592 90-91 22.54591892498231 26.77155298868493 26.906528454489496 23.775999631843256 92-93 22.848276714111947 26.334696176347638 27.182494810253004 23.63453229928741 94-95 22.52041276735853 26.022364022450073 27.49692430842094 23.960298901770454 96-97 22.88205167852349 25.565908317997472 27.445466221754057 24.10657378172499 98-99 22.60424081435268 25.876739110729307 26.97750296897496 24.541517105943058 100-101 23.034647303143462 25.368495354297433 27.317905616642314 24.27895172591679 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 392.0 1 290.0 2 264.5 3 358.0 4 314.0 5 301.5 6 543.0 7 1163.5 8 1874.0 9 2104.5 10 1816.0 11 1555.0 12 1577.5 13 1707.0 14 1895.5 15 2041.0 16 2180.0 17 2410.0 18 2656.0 19 2946.5 20 3350.5 21 4036.5 22 4905.5 23 5852.0 24 7128.0 25 8883.0 26 11378.0 27 14549.0 28 17335.0 29 19932.5 30 22909.5 31 27934.0 32 35790.0 33 38754.5 34 37876.5 35 39795.0 36 43827.5 37 48260.5 38 51554.0 39 54386.5 40 57587.5 41 61542.0 42 63761.0 43 65883.0 44 68793.0 45 73154.5 46 75705.0 47 77293.0 48 80239.5 49 79666.0 50 77826.0 51 76724.0 52 75996.5 53 77378.0 54 79424.0 55 76968.0 56 72054.0 57 66826.5 58 63210.5 59 56902.5 60 46094.5 61 38362.5 62 33107.0 63 25658.5 64 18294.0 65 13784.0 66 10493.0 67 8177.0 68 6374.5 69 5055.0 70 4030.5 71 2865.5 72 2076.5 73 1675.0 74 1243.0 75 902.5 76 686.5 77 466.5 78 286.0 79 185.5 80 127.5 81 80.5 82 46.5 83 26.5 84 19.0 85 16.5 86 11.5 87 9.0 88 6.0 89 2.0 90 0.5 91 1.0 92 1.5 93 2.5 94 2.0 95 0.0 96 1.0 97 1.5 98 0.5 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18107041320489112 2 0.04512959231554831 3 0.043427456825563315 4 0.04839585230984386 5 0.05667651145031145 6 0.06440512664808119 7 0.03565283796590209 8 0.0356068343040106 9 0.039977182183701825 10-11 0.04103526640720601 12-13 0.03786101373669344 14-15 0.03296162374525012 16-17 0.030661440650675793 18-19 0.038528066834119995 20-21 0.049522942026185277 22-23 0.04584264907486636 24-25 0.0662682749546864 26-27 0.0510180610376586 28-29 0.04287541288286548 30-31 0.05235216723251171 32-33 0.048211837662277914 34-35 0.04747577907201413 36-37 0.04489957400609089 38-39 0.02468096460478254 40-41 0.023461867564658144 42-43 0.020471629541711518 44-45 0.0046693716819858865 46-47 0.013157047300965157 48-49 0.012420988710701374 50-51 0.009936790968561099 52-53 0.009959792799506841 54-55 0.011247895332468465 56-57 0.008855704914111162 58-59 0.011293898994359952 60-61 0.010534838573150422 62-63 0.004462355203474196 64-65 0.0074525932264208235 66-67 0.004715375343877373 68-69 0.008809701252219675 70-71 0.021690726581835913 72-73 0.018815497713618005 74-75 0.019597559965773278 76-77 0.011868944768003533 78-79 0.005198413793737981 80-81 0.007245576747909133 82-83 0.005865466891164536 84-85 0.005842465060218793 86-87 0.008096644492901636 88-89 0.02783221544434937 90-91 0.03461775557334364 92-93 0.02136870094859551 94-95 0.029971385722303498 96-97 0.033536669518893704 98-99 0.019827578275230708 100-101 0.12179469485771068 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173740.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.456765107028275 #Duplication Level Percentage of deduplicated Percentage of total 1 73.67601464668782 36.43777350403214 2 13.886877250672189 13.73600052513258 3 5.089445930054165 7.551225956628298 4 2.4409998973632407 4.828958342006957 5 1.3260671123978167 3.279149484700704 6 0.8150888271272161 2.4186993998756345 7 0.5359096369639998 1.8553049923744933 8 0.3799811443694387 1.5034110561743304 9 0.2749878500616754 1.2240008557008293 >10 1.4125043502791488 12.759419641527314 >50 0.09949175370224286 3.4162548232043464 >100 0.05428999839158429 5.112327454999053 >500 0.004123938032603168 1.4448009871983403 >1k 0.004030212168225808 3.8402253067289096 >5k 1.8745172875468875E-4 0.5924476697161262 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7123 0.3276840836530588 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5658 0.26028871898203093 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4006 0.18429066953729517 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3960 0.1821745010902868 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3727 0.17145564786957043 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3712 0.1707655929411981 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3300 0.15181208424190565 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2926 0.13460671469448968 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2914 0.13405467075179184 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2801 0.12885625695805386 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2747 0.12637205921591357 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2540 0.11684930120437587 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2515 0.1156992096570887 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2232 0.10268017334179802 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.600366189148656E-5 2 0.0 0.0 0.0 0.0 4.600366189148656E-5 3 0.0 0.0 0.0 0.0 4.600366189148656E-5 4 0.0 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 5 0.0 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 6 4.600366189148656E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 7 4.600366189148656E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 8 4.600366189148656E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 9 4.600366189148656E-5 0.0 0.0 9.200732378297312E-5 4.600366189148656E-5 10-11 4.600366189148656E-5 0.0 0.0 9.200732378297312E-5 4.600366189148656E-5 12-13 9.200732378297312E-5 0.0 0.0 1.150091547287164E-4 3.6802929513189247E-4 14-15 1.8401464756594624E-4 0.0 0.0 1.3801098567445968E-4 3.6802929513189247E-4 16-17 2.0701647851168953E-4 0.0 0.0 1.8401464756594624E-4 3.6802929513189247E-4 18-19 2.530201404031761E-4 0.0 0.0 2.530201404031761E-4 4.1403295702337905E-4 20-21 2.7602197134891937E-4 0.0 0.0 3.680292951318925E-4 4.6003661891486563E-4 22-23 2.7602197134891937E-4 0.0 0.0 4.830384498606089E-4 4.6003661891486563E-4 24-25 3.2202563324040595E-4 0.0 0.0 8.97071406883988E-4 4.6003661891486563E-4 26-27 3.2202563324040595E-4 0.0 0.0 0.002461195911194531 5.290421117520954E-4 28-29 4.3703478796912234E-4 0.0 0.0 0.0057734595673815635 5.520439426978387E-4 30-31 5.060402808063522E-4 0.0 0.0 0.016722331097555367 5.520439426978387E-4 32-33 5.060402808063522E-4 0.0 0.0 0.039287127255329526 5.520439426978387E-4 34-35 5.060402808063522E-4 0.0 0.0 0.07383587733583594 5.980476045893253E-4 36-37 6.440512664808119E-4 0.0 0.0 0.13258255357126428 5.980476045893253E-4 38-39 7.820622521552715E-4 0.0 0.0 0.221668644824128 6.440512664808119E-4 40-41 9.430750687754745E-4 0.0 0.0 0.31774729268449764 6.440512664808119E-4 42-43 0.0011040878853956775 0.0 0.0 0.416011114484713 6.440512664808119E-4 44-45 0.0011500915472871642 0.0 0.0 0.5395079448324087 7.590604212095283E-4 46-47 0.0011960952091786506 0.0 0.0 0.6850865328880179 8.740695759382447E-4 48-49 0.0012881025329616238 0.0 0.0 0.8403028881098935 9.200732378297313E-4 50-51 0.0012881025329616238 0.0 0.0 1.0113215011914949 0.0011040878853956775 52-53 0.0012881025329616238 0.0 0.0 1.177693744422056 0.0011500915472871642 54-55 0.0013341061948531103 0.0 0.0 1.3566709910108843 0.0011960952091786506 56-57 0.001380109856744597 0.0 0.0 1.5629284091013644 0.001242098871070137 58-59 0.001380109856744597 0.0 0.0 1.7831709404068565 0.0012881025329616238 60-61 0.0014031116876903403 0.0 0.0 2.0254262239274246 0.0013341061948531103 62-63 0.0014721171805275699 0.0 0.0 2.2668074378720546 0.001380109856744597 64-65 0.001564124504310543 0.0 0.0 2.515572239550268 0.001380109856744597 66-67 0.0016101281662020297 0.0 0.0 2.80606236256406 0.001380109856744597 68-69 0.0016101281662020297 0.0 0.0 3.1303651770680947 0.001380109856744597 70-71 0.0016791336590392596 0.0 0.0 3.4681470645063346 0.0014261135186360834 72-73 0.0017481391518764894 0.0 0.0 3.8211791658616026 0.0014491153495818265 74-75 0.0017481391518764894 0.0 0.0 4.171428045672435 0.0014951190114733132 76-77 0.0017481391518764894 0.0 0.0 4.557237756125387 0.0015181208424190566 78-79 0.0017481391518764894 0.0 0.0 4.987464002134569 0.0015181208424190566 80-81 0.0018401464756594625 0.0 0.0 5.435999705576563 0.0015411226733648 82-83 0.0018401464756594625 0.0 0.0 5.910964512775217 0.001564124504310543 84-85 0.0018631483066052059 0.0 0.0 6.380017849420813 0.001564124504310543 86-87 0.001886150137550949 0.0 0.0 6.873545134192682 0.0016331299971477729 88-89 0.001886150137550949 0.0 0.0 7.390442279205425 0.001702135489985003 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4755 0.0 49.86322 1 GTATCAA 8435 0.0 40.470222 1 TATCAAC 10790 0.0 31.540052 2 TCAACGC 10905 0.0 31.251028 4 ATCAACG 10895 0.0 31.236088 3 CAACGCA 11095 0.0 30.713737 5 TAAGGTG 1565 0.0 30.065033 5 AACGCAG 11475 0.0 29.691168 6 AAGGTAA 1470 0.0 26.558165 1 GTAAGGT 1635 0.0 26.163483 4 ACGCAGA 13065 0.0 25.706375 7 AGGTAAG 1505 0.0 25.581108 2 CGCAGAG 13150 0.0 25.467962 8 TACATGG 9390 0.0 24.650978 2 GGTAAGG 1660 0.0 24.624146 3 TAAGGTA 755 0.0 24.552092 4 GTACATG 9760 0.0 24.292995 1 GTGTAAG 995 0.0 23.446312 1 ACATGGG 9910 0.0 22.542135 3 GCAGAGT 14155 0.0 22.516119 9 >>END_MODULE