FastQCFastQC Report
Thu 26 May 2016
SRR938462_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938462_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2173740
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71450.32869616421467146No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60120.2765740152916172No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41550.19114521515912666No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40840.18787895516483114No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38980.17932227405301462No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35710.16427907661449853No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35260.1622089118293816No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32310.1486378315713931No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG30820.14178328594956158No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA26630.1225077516170287No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG26120.1201615648605629No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24670.11349103388629736No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24060.11068481051091666No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT23600.10856864206390827No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA23430.107786579811753No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT22350.10281818432747247No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49050.047.9996831
GTATCAA82000.038.7467041
TAAGGTG14050.031.1288935
TATCAAC107050.029.4132842
ATCAACG107950.029.1973233
TCAACGC108550.029.123534
CAACGCA110700.028.5578965
GTAAGGT15800.028.2828354
AGGTAAG14400.028.0755732
AACGCAG114900.027.4886886
AAGGTAA15150.025.1159461
TACATGG97000.024.8113842
GTACATG100000.024.2572961
ACGCAGA132850.023.738817
GGTAAGG17950.023.5709843
CGCAGAG133650.023.5611748
ACATGGG98500.023.3111173
GTACCGT2405.9408194E-921.7687666
TAAGGTA6850.021.5141054
GCAGAGT148500.020.853249
Produced by FastQC (version 0.11.5)