##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938462_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2173740 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.74774490049408 31.0 31.0 34.0 30.0 34.0 2 31.969138903456717 31.0 31.0 34.0 30.0 34.0 3 32.02898046684516 33.0 31.0 34.0 30.0 34.0 4 35.723612759575666 37.0 35.0 37.0 33.0 37.0 5 35.58713829620838 37.0 35.0 37.0 33.0 37.0 6 35.61770634942541 37.0 35.0 37.0 33.0 37.0 7 35.545788824790456 37.0 35.0 37.0 33.0 37.0 8 35.533981064892764 37.0 35.0 37.0 33.0 37.0 9 37.10126740088511 39.0 37.0 39.0 33.0 39.0 10-11 37.00550019781575 39.0 37.0 39.0 33.0 39.0 12-13 36.90056745516944 39.0 37.0 39.0 33.0 39.0 14-15 37.934081352875694 40.0 37.0 41.0 33.0 41.0 16-17 37.85981465124624 40.0 37.0 41.0 32.5 41.0 18-19 37.979634408898946 40.0 37.0 41.0 33.0 41.0 20-21 38.018841949819205 40.0 37.0 41.0 33.0 41.0 22-23 37.926666712670325 40.0 37.0 41.0 32.5 41.0 24-25 37.82208106765299 40.0 37.0 41.0 32.0 41.0 26-27 37.62678149180675 40.0 37.0 41.0 32.0 41.0 28-29 37.5027337676079 39.5 36.5 41.0 32.0 41.0 30-31 37.243302556883535 39.0 36.0 41.0 31.0 41.0 32-33 37.00991861952211 39.0 36.0 41.0 31.0 41.0 34-35 36.8514422148003 39.0 35.0 40.5 30.0 41.0 36-37 36.82617631363456 39.0 35.0 41.0 30.0 41.0 38-39 36.6877786671819 39.0 35.0 41.0 30.0 41.0 40-41 36.492789616053436 39.0 35.0 40.5 30.0 41.0 42-43 36.25375067855401 39.0 35.0 40.0 30.0 41.0 44-45 35.9699897411834 38.0 35.0 40.0 29.0 41.0 46-47 35.58176989888395 38.0 34.5 40.0 27.5 41.0 48-49 35.19089725542153 38.0 34.0 40.0 26.5 41.0 50-51 34.79903346306366 38.0 34.0 40.0 26.0 41.0 52-53 34.28962548418854 38.0 33.0 40.0 24.0 41.0 54-55 33.96602859587624 37.0 33.0 40.0 23.5 41.0 56-57 33.61562100343187 37.0 32.5 40.0 23.0 41.0 58-59 33.1636274347438 36.0 32.0 39.0 22.0 41.0 60-61 32.78211469632983 36.0 31.0 39.0 21.5 40.0 62-63 32.31951935374056 35.0 31.0 39.0 19.5 40.0 64-65 31.808552540782244 35.0 30.5 38.0 18.0 40.0 66-67 31.317734871695787 35.0 30.0 38.0 16.0 40.0 68-69 30.788695520163404 34.0 29.0 37.0 12.5 39.5 70-71 30.20850055664431 34.0 29.0 36.5 8.0 39.0 72-73 29.777863957971057 34.0 29.0 36.0 7.5 39.0 74-75 29.287533467664026 34.0 28.0 35.5 7.0 38.0 76-77 28.053534691361435 31.5 26.5 34.5 3.5 36.0 78-79 28.76238740603752 33.0 28.0 35.0 2.0 37.0 80-81 28.517371212748536 33.0 27.0 35.0 2.0 36.5 82-83 28.069304976676143 33.0 26.5 35.0 2.0 36.0 84-85 27.570870021253693 33.0 26.0 35.0 2.0 36.0 86-87 27.02683117576159 32.0 25.5 35.0 2.0 35.0 88-89 26.387848132711362 32.0 24.5 34.0 2.0 35.0 90-91 25.8645320967549 32.0 23.5 34.0 2.0 35.0 92-93 25.162417768454368 31.0 20.0 34.0 2.0 35.0 94-95 24.406864436409137 31.0 17.5 34.0 2.0 35.0 96-97 23.428699844507623 31.0 4.5 34.0 2.0 35.0 98-99 22.19243170756392 30.0 2.0 34.0 2.0 35.0 100-101 18.547594468519694 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3353132404192358 1101 2 0.3568998434393471 1101 3 0.32977042543247137 1101 4 0.2597406425820168 1101 5 0.15496742844936762 1101 6 0.16586748120251826 1101 7 0.38822944818437577 1101 8 0.28644922126002825 1101 9 0.3323415868715003 1101 10-11 0.3237969082118326 1101 12-13 0.3333244772392874 1101 14-15 0.46473450991371834 1101 16-17 0.4065584181223727 1101 18-19 0.5445842666639393 1101 20-21 0.46328588201608767 1101 22-23 0.46383130337200384 1101 24-25 0.48874325383604145 1101 26-27 0.5019529272394152 1101 28-29 0.5234438034613689 1101 30-31 0.5484012223197823 1101 32-33 0.5505652235124714 1101 34-35 0.4252561957616976 1101 36-37 0.5185270733084479 1101 38-39 0.5409105895748212 1101 40-41 0.5341080041615527 1101 42-43 0.7194852207975302 1101 44-45 0.6450093948935702 1101 46-47 0.7449345089536408 1101 48-49 0.7767513276755622 1101 50-51 0.803457139197242 1101 52-53 0.9044270363657745 1101 54-55 0.921643506368909 1101 56-57 0.799217228771063 1101 58-59 0.9355960511089094 1101 60-61 0.8217213964090675 1101 62-63 0.6793196594276267 1101 64-65 0.754240848715046 1101 66-67 0.4510459897429122 1101 68-69 0.6254029996116053 1101 70-71 0.42629265661561533 1101 72-73 0.47906899238998335 1101 74-75 0.5754496281261652 1101 76-77 0.5928184486655113 1101 78-79 0.6069405487783328 1101 80-81 0.7005229466646767 1101 82-83 0.8059608273820906 1101 84-85 0.8675651963012214 1101 86-87 0.8593415273526119 1101 88-89 0.8331889299949999 1101 90-91 0.7061116932430167 1101 92-93 0.8650821963463038 1101 94-95 1.0756870122170383 1101 96-97 1.3091755333643462 1101 98-99 1.0220416784914619 1101 100-101 0.5813160250580118 1102 1 0.3373586049926409 1102 2 0.32100097221248447 1102 3 0.3870558192463136 1102 4 0.24540685751600932 1102 5 0.22875214885336703 1102 6 0.3015305018907597 1102 7 0.329194288440668 1102 8 0.35516530203569374 1102 9 0.38726271518383015 1102 10-11 0.4318437107194768 1102 12-13 0.4141416438490495 1102 14-15 0.48365033577810124 1102 16-17 0.47434463301446783 1102 18-19 0.4909407057397246 1102 20-21 0.47338611325853464 1102 22-23 0.4794164734839157 1102 24-25 0.5281022524975967 1102 26-27 0.5133709157652646 1102 28-29 0.521030053333071 1102 30-31 0.489945215985756 1102 32-33 0.563806288526365 1102 34-35 0.5434274481783277 1102 36-37 0.4856648301310429 1102 38-39 0.4183133431193866 1102 40-41 0.5631121483026789 1102 42-43 0.4589942180976081 1102 44-45 0.4362836318877541 1102 46-47 0.4870583367701826 1102 48-49 0.5465992449226107 1102 50-51 0.5561674043510578 1102 52-53 0.308764350811515 1102 54-55 0.27667253211348 1102 56-57 0.34915114453932716 1102 58-59 0.32609468857981483 1102 60-61 0.41509495978394995 1102 62-63 0.29908724924876395 1102 64-65 0.49348723712275344 1102 66-67 0.4070364834581035 1102 68-69 0.5102713862137875 1102 70-71 0.5500103764059077 1102 72-73 0.2596040180800969 1102 74-75 0.1788649052086555 1102 76-77 0.3132418276119786 1102 78-79 0.3642395312277884 1102 80-81 0.3590578142134433 1102 82-83 0.2455075010726091 1102 84-85 0.18378400527694794 1102 86-87 0.12505551458819752 1102 88-89 0.3573996109244071 1102 90-91 0.16957750748244393 1102 92-93 0.356391491496705 1102 94-95 0.3513687480434875 1102 96-97 0.38530998806994177 1102 98-99 0.13116994264406756 1102 100-101 -0.1364578317685634 1103 1 0.24837834442032047 1103 2 0.22419026705326317 1103 3 0.2857840488462031 1103 4 0.1238454497477477 1103 5 0.08210641926658724 1103 6 0.09432327894754167 1103 7 0.18480354523838116 1103 8 0.13536613730640568 1103 9 0.35528417860187744 1103 10-11 0.41174184332525954 1103 12-13 0.2742434064414283 1103 14-15 0.4381008334995329 1103 16-17 0.48724399354853887 1103 18-19 0.415288619920986 1103 20-21 0.38489318126968897 1103 22-23 0.4349796174694234 1103 24-25 0.43806349328158944 1103 26-27 0.3515673458650568 1103 28-29 0.33160508359460295 1103 30-31 0.3448819942721997 1103 32-33 0.41534574030790594 1103 34-35 0.3817540072367862 1103 36-37 0.3775818130955031 1103 38-39 0.4672278580003777 1103 40-41 0.40531026221879074 1103 42-43 0.418366909181934 1103 44-45 0.46912503700102803 1103 46-47 0.3889341151422272 1103 48-49 0.6109694120291564 1103 50-51 0.6288501045356796 1103 52-53 0.6029343987932947 1103 54-55 0.7597243269324281 1103 56-57 0.7351223694458326 1103 58-59 0.7436514431461134 1103 60-61 0.6621129105934571 1103 62-63 0.7341808460306467 1103 64-65 0.7147186937461463 1103 66-67 0.755242363190991 1103 68-69 0.6609685804937051 1103 70-71 0.4745495539559528 1103 72-73 0.4962981137187654 1103 74-75 0.6195531738646949 1103 76-77 0.515388868095183 1103 78-79 0.6486298202887468 1103 80-81 0.7459530420613909 1103 82-83 0.7745716729895555 1103 84-85 0.765766995937355 1103 86-87 0.8357360453746132 1103 88-89 0.619265363253195 1103 90-91 0.6877562610042212 1103 92-93 0.5218963132549632 1103 94-95 0.6095655624167122 1103 96-97 0.7115494009001324 1103 98-99 0.477030007726011 1103 100-101 0.25493776201249574 1104 1 0.18989875841770498 1104 2 0.2013412825243428 1104 3 0.19169463489082972 1104 4 0.15033003650339793 1104 5 0.023277696474586662 1104 6 0.06303316303601747 1104 7 0.11533405847181655 1104 8 0.11194352032433841 1104 9 0.21167871686552076 1104 10-11 0.22765752360376723 1104 12-13 0.20415850411711034 1104 14-15 0.39880394164317323 1104 16-17 0.2842298028731136 1104 18-19 0.24152637947786815 1104 20-21 0.32292907319261843 1104 22-23 0.3426911261416592 1104 24-25 0.32931694392866007 1104 26-27 0.4443513328797124 1104 28-29 0.41855064894361504 1104 30-31 0.39746662158343327 1104 32-33 0.3555135148170123 1104 34-35 0.3501326953039552 1104 36-37 0.44408151258764406 1104 38-39 0.4965960691311082 1104 40-41 0.5157392307048809 1104 42-43 0.6655041763591569 1104 44-45 0.6882224180737992 1104 46-47 0.4731512673663403 1104 48-49 0.5918015898353843 1104 50-51 0.8002968055825264 1104 52-53 0.8444101733269491 1104 54-55 1.0063498100938375 1104 56-57 0.9024057500153191 1104 58-59 0.8853348820703175 1104 60-61 0.6270503940985748 1104 62-63 0.6081558026069089 1104 64-65 0.632993419783535 1104 66-67 0.47032974653469495 1104 68-69 0.5279946762851289 1104 70-71 0.4062405516090166 1104 72-73 0.36377390902593376 1104 74-75 0.4626223246667678 1104 76-77 0.21539550145812214 1104 78-79 0.2680274023453322 1104 80-81 0.30147676849111704 1104 82-83 0.2729389450627231 1104 84-85 0.39692392525214615 1104 86-87 0.44854051319241606 1104 88-89 0.4285069597738165 1104 90-91 0.4440140481237904 1104 92-93 0.6136143589765375 1104 94-95 0.7037648635765805 1104 96-97 0.4755224264831952 1104 98-99 0.1997361979047625 1104 100-101 0.04691349381878851 1105 1 0.29629014411198895 1105 2 0.3169213482044988 1105 3 0.236855629775107 1105 4 0.21361718438841848 1105 5 0.14679040728564274 1105 6 0.1883030142743891 1105 7 0.19283402576257913 1105 8 0.22166209001464665 1105 9 0.30691101789520303 1105 10-11 0.3092984808070014 1105 12-13 0.29759506781508094 1105 14-15 0.3579474828764475 1105 16-17 0.3706149083931223 1105 18-19 0.40156549973011835 1105 20-21 0.3541598565135118 1105 22-23 0.3677431338348782 1105 24-25 0.4103482008793762 1105 26-27 0.25218715865721464 1105 28-29 0.32012003832750935 1105 30-31 0.49973271780093853 1105 32-33 0.34961669284686536 1105 34-35 0.4674417714641521 1105 36-37 0.5256544345695602 1105 38-39 0.3970304478517832 1105 40-41 0.2970897298479045 1105 42-43 0.41437570754415276 1105 44-45 0.33543101144559984 1105 46-47 0.25307695197074764 1105 48-49 0.14859595337897247 1105 50-51 0.06967310577485364 1105 52-53 0.11546314921369571 1105 54-55 0.09510241047850343 1105 56-57 0.2441754507911824 1105 58-59 0.4562851509402819 1105 60-61 0.19469833799568192 1105 62-63 0.19800677930484056 1105 64-65 0.2040352745283407 1105 66-67 0.29829418504786176 1105 68-69 0.10897032752611935 1105 70-71 0.08198076173318469 1105 72-73 0.12684059662651492 1105 74-75 0.20287104573544212 1105 76-77 0.19914293378269932 1105 78-79 0.37417870221057115 1105 80-81 0.48479178464948447 1105 82-83 0.39177881370240897 1105 84-85 0.6013854657265618 1105 86-87 0.6125930573171807 1105 88-89 0.418153830807821 1105 90-91 0.585989817118044 1105 92-93 0.5888724348881489 1105 94-95 0.44267577668775715 1105 96-97 0.44868580260021673 1105 98-99 0.1134819293488114 1105 100-101 -0.13260594201222276 1106 1 0.16535057325942049 1106 2 0.15529913530972905 1106 3 0.15232096346457524 1106 4 0.12119785621909784 1106 5 -0.005130835130501055 1106 6 -0.04033818845771009 1106 7 0.14171663982243388 1106 8 0.1554914740214599 1106 9 0.10396015235037481 1106 10-11 -0.009635391822449435 1106 12-13 -0.0037900582962606677 1106 14-15 0.11883770304662988 1106 16-17 0.011070384665366362 1106 18-19 0.23345583437353667 1106 20-21 0.20268633162830696 1106 22-23 0.26416393743696176 1106 24-25 0.27931069181691015 1106 26-27 0.0913338243063393 1106 28-29 0.22946550582346958 1106 30-31 0.28586110589063196 1106 32-33 0.2983193295469775 1106 34-35 0.031425312757185964 1106 36-37 -0.22083123719752962 1106 38-39 0.08508007835762754 1106 40-41 0.12122831877452711 1106 42-43 0.18698417093180097 1106 44-45 0.15635926216742035 1106 46-47 -0.03571675454531942 1106 48-49 -0.07083778569837307 1106 50-51 -0.07303940106173457 1106 52-53 -0.02307299252960604 1106 54-55 -0.4376299383850437 1106 56-57 -0.06321761213093424 1106 58-59 -0.12018360422691643 1106 60-61 -0.3492294929909505 1106 62-63 -0.3149019855289765 1106 64-65 -0.6476525696863114 1106 66-67 -0.5222009752782562 1106 68-69 -1.101289017941376 1106 70-71 -0.8149551714144501 1106 72-73 -0.5995454834284324 1106 74-75 -0.2976943248398527 1106 76-77 -0.34333045119078065 1106 78-79 -0.49207840466927166 1106 80-81 -1.2046862174110338 1106 82-83 -0.5442153863204844 1106 84-85 -0.4555102745972661 1106 86-87 -0.3259850579446457 1106 88-89 -0.4946805264249754 1106 90-91 -0.4619089631060689 1106 92-93 -0.7394743172518652 1106 94-95 -0.49086869616301243 1106 96-97 -0.44081700199397744 1106 98-99 -0.3728893963784152 1106 100-101 -0.5627219415543294 1107 1 0.17239843879433536 1107 2 0.2128201485256902 1107 3 0.19248429263060274 1107 4 0.1486667415499383 1107 5 0.12038429024205755 1107 6 0.2050637616467199 1107 7 0.16408727664046552 1107 8 0.1869948480774184 1107 9 0.38145889119186904 1107 10-11 0.2963746682013806 1107 12-13 0.25632479851655887 1107 14-15 0.4060430976369318 1107 16-17 0.3924392351468029 1107 18-19 0.34278243665410457 1107 20-21 0.429136785767227 1107 22-23 0.355695410348261 1107 24-25 0.3539962551465621 1107 26-27 0.33709889058113873 1107 28-29 0.4119338184543082 1107 30-31 0.41777523988294263 1107 32-33 0.512194144637995 1107 34-35 0.40801272749190787 1107 36-37 0.3904023342327889 1107 38-39 0.4193443538190209 1107 40-41 0.6178018668581728 1107 42-43 0.5854767514205221 1107 44-45 0.5496719539305346 1107 46-47 0.5856073034850269 1107 48-49 0.619036634595183 1107 50-51 0.698314388628198 1107 52-53 0.8051328480355835 1107 54-55 0.8529099105683855 1107 56-57 0.7120957278798343 1107 58-59 0.6302002266243107 1107 60-61 0.45132773192418085 1107 62-63 0.6989463878811719 1107 64-65 0.7404576909043747 1107 66-67 0.585370971682412 1107 68-69 0.7697590474292824 1107 70-71 0.5227069209632802 1107 72-73 0.46011340670333567 1107 74-75 0.6714103379129597 1107 76-77 0.7651827952383741 1107 78-79 0.6991046960503837 1107 80-81 0.7988170006755588 1107 82-83 0.668178938344365 1107 84-85 0.7022789328103016 1107 86-87 0.683689307272239 1107 88-89 0.683385916523136 1107 90-91 0.6760148653568798 1107 92-93 0.3564535420442745 1107 94-95 0.36033957898644076 1107 96-97 0.41294831773102914 1107 98-99 0.3234047472157542 1107 100-101 -0.1327869125415937 1108 1 0.18816999708282367 1108 2 0.16944554193000627 1108 3 0.22780087718302866 1108 4 0.058208711243601385 1108 5 0.06934932177752273 1108 6 0.07308457692850112 1108 7 0.09424981043392933 1108 8 0.06468249719998909 1108 9 0.19366401727825888 1108 10-11 0.13335723656966536 1108 12-13 0.09358940148185013 1108 14-15 0.2858566698035432 1108 16-17 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-0.34015944521034314 2316 88-89 -0.03499029922361885 2316 90-91 -0.1008848287507007 2316 92-93 -0.18891145338969295 2316 94-95 -0.14205519465955518 2316 96-97 0.16350709824842724 2316 98-99 0.10156089922960021 2316 100-101 0.1609608124234434 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 15.0 4 51.0 5 82.0 6 165.0 7 483.0 8 1099.0 9 1866.0 10 3114.0 11 4873.0 12 6667.0 13 9433.0 14 11970.0 15 15903.0 16 19595.0 17 24440.0 18 22753.0 19 19365.0 20 18682.0 21 19590.0 22 21094.0 23 24138.0 24 28260.0 25 33113.0 26 39815.0 27 47611.0 28 57462.0 29 68865.0 30 81906.0 31 97707.0 32 117857.0 33 145175.0 34 184242.0 35 240420.0 36 310961.0 37 334054.0 38 154312.0 39 6601.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.73075020989315 19.05346812499281 14.975675395921748 23.240106269192285 2 16.21523475686652 20.591094280154422 38.479304244088965 24.71436671889009 3 19.362714315879835 23.94858765093372 30.16061413641782 26.528083896768628 4 13.544168115782016 16.837570270593538 35.71241270805156 33.90584890557288 5 13.796226505444938 37.624808168952214 33.77276245457178 14.80620287103106 6 33.05054882368636 35.82019928786331 16.563618464029737 14.565633424420584 7 29.246276003569882 32.86197981359316 19.7876470967089 18.104097086128057 8 27.42742922336618 34.174924323976185 19.209243055747237 19.188403396910395 9 26.97282103655451 16.149999539963382 19.453844526024273 37.423334897457835 10-11 25.475286832831895 26.27085115975232 26.739076430483866 21.514785576931924 12-13 26.521525113399026 23.54906750577346 27.087140136354854 22.842267244472662 14-15 22.92176157222115 24.928924342377652 25.531273289353834 26.618040796047364 16-17 22.47982739426058 28.130089155096744 26.573187225703165 22.816896224939505 18-19 22.665199149852327 26.30903419912225 28.909069161905286 22.116697489120135 20-21 23.070698427594838 25.482854435213042 29.2226991268505 22.223748010341623 22-23 22.869800436114716 25.549858768757993 29.1837800288903 22.39656076623699 24-25 22.76022606251452 25.857705746100606 29.125514379627692 22.256553811757183 26-27 22.049543202790815 26.16475524572044 29.014783367819806 22.77091818366894 28-29 22.377377239228245 26.567206749657274 28.7906557361966 22.264760274917883 30-31 22.345358690551766 26.036508506077084 28.613978672702345 23.0041541306688 32-33 21.94694397674055 26.04715835380496 28.683329193003765 23.322568476450726 34-35 23.074681039160968 26.167705734083246 28.35672912419971 22.40088410255608 36-37 23.19725817859792 25.637755248736337 28.407224883409338 22.757761689256405 38-39 22.50573284362155 25.982864381472808 28.48261192702905 23.028790847876586 40-41 22.890170006482265 25.954523852572407 28.54453141138091 22.61077472956442 42-43 22.690934919975167 25.938599132413547 28.265657465054904 23.104808482556376 44-45 22.557412570040576 26.121845298885795 28.636267446888773 22.68447468418486 46-47 23.22835297689696 26.500179414281376 27.653537221562836 22.617930387258824 48-49 22.748764801678213 26.67076099257501 27.423610919429187 23.15686328631759 50-51 23.04939873213908 26.7310487914838 27.54080524809775 22.678747228279374 52-53 23.470631262248475 26.29877538252045 27.507061562100343 22.723531793130732 54-55 22.68955808882387 26.68874842437458 27.71327297652893 22.908420510272617 56-57 22.790191099211494 26.793866791796628 27.406658570022174 23.0092835389697 58-59 22.7129049472338 26.268481971164903 27.477619218489792 23.540993863111503 60-61 22.263357163230193 26.722699126850497 27.80886858593944 23.205075123979867 62-63 22.48389871833798 26.728058553460855 27.894067367762474 22.893975360438688 64-65 22.149176074415525 26.799226218406986 27.60978773910403 23.44180996807346 66-67 22.03924994554305 26.805561210184038 27.61071896741807 23.544469876854844 68-69 22.782604255248675 26.253504655968086 27.715402498625053 23.248488590158185 70-71 22.590139740980504 26.412614043287686 27.219059720223616 23.778186495508194 72-73 22.388625134560712 26.62422828857177 27.34280548731679 23.64434108955073 74-75 22.322126841296566 27.04382768868402 27.10926789772466 23.524777572294756 76-77 22.440379254188635 26.517430787490685 27.513709091243665 23.528480867077022 78-79 22.370729710084923 26.792279665461372 27.45119011473313 23.385800509720575 80-81 22.62964422322496 26.729987024479136 27.13634325853719 23.504025493758707 82-83 22.460896033512988 26.748651910580207 27.307577386186704 23.482874669720104 84-85 22.240525060665053 26.921251711721784 27.237130946112593 23.60109228150057 86-87 22.56428752654183 26.955987084643795 26.751059200443116 23.728666188371257 88-89 22.277086751025195 27.04948802477575 26.588990945550933 24.084434278648118 90-91 22.01494846155244 26.908817972933775 26.916063788015514 24.16016977749827 92-93 22.21391335990687 26.51116187414504 27.145812252885783 24.129112513062307 94-95 21.819827913983215 26.233836314717706 27.424250893104208 24.522084878194867 96-97 22.049302124449106 25.79259709072842 27.47085668019174 24.68724410463073 98-99 21.720175899844367 26.20006468123789 27.03406621868239 25.045693200235352 100-101 21.745194917515434 25.86440880694103 27.41556027859818 24.97483599694536 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 413.0 1 301.0 2 241.5 3 301.5 4 279.0 5 275.5 6 496.5 7 1102.5 8 1784.5 9 2082.0 10 1818.5 11 1510.5 12 1490.5 13 1598.0 14 1756.0 15 1946.5 16 2174.5 17 2356.0 18 2642.5 19 2997.0 20 3340.5 21 3911.5 22 4652.5 23 5635.0 24 6870.5 25 8585.0 26 11255.5 27 14413.0 28 17030.5 29 19662.5 30 22991.0 31 28388.5 32 37254.5 33 39860.5 34 38171.0 35 40148.5 36 44058.0 37 48604.0 38 51964.0 39 54688.0 40 57991.0 41 62143.0 42 64348.0 43 66836.0 44 70085.0 45 74445.5 46 76783.5 47 78698.0 48 81190.5 49 79929.5 50 78746.5 51 77820.5 52 76317.5 53 77979.5 54 81155.5 55 78310.5 56 72456.0 57 66593.0 58 62344.0 59 56119.5 60 44978.5 61 36954.0 62 32044.5 63 24230.5 64 16432.5 65 12095.0 66 9048.0 67 7096.5 68 5590.0 69 4364.5 70 3356.5 71 2223.5 72 1615.0 73 1329.5 74 1007.0 75 781.5 76 552.5 77 343.5 78 218.5 79 131.5 80 84.0 81 52.5 82 33.0 83 19.0 84 10.5 85 4.5 86 3.0 87 1.0 88 0.5 89 0.5 90 0.0 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.900549283722985E-4 2 0.047107749776882236 3 9.660768997212178E-4 4 0.0 5 0.08772898322706488 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 2.3001830945743282E-4 26-27 0.008395668295196298 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010879866037336572 36-37 1.1500915472871641E-4 38-39 0.003703294782264668 40-41 0.005382428441303928 42-43 3.9103112607763576E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 2.0701647851168953E-4 68-69 0.0011270897163414208 70-71 1.8401464756594624E-4 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0014491153495818268 82-83 7.130567593180417E-4 84-85 0.005727455905490077 86-87 0.002231177601737098 88-89 9.200732378297312E-5 90-91 0.0032892618252412893 92-93 0.0010810860544499341 94-95 6.900549283722984E-5 96-97 0.0 98-99 1.3801098567445968E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2173740.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.65941072268588 #Duplication Level Percentage of deduplicated Percentage of total 1 71.51472647535812 33.36834995334272 2 14.666174198696352 13.686300913348632 3 5.568876704287122 7.795215162279906 4 2.6872606859388313 5.015440002565861 5 1.4847600090781163 3.4639013544097326 6 0.9613399665921661 2.69133338072142 7 0.6037965832044674 1.9720954938084136 8 0.4207873050494606 1.5706950154555914 9 0.30127930208998677 1.2651763228614765 >10 1.6063723974289592 13.661028475596805 >50 0.1112238981706964 3.5864623741524473 >100 0.06413157645808934 5.73353659332945 >500 0.004536822253113357 1.4954656874708805 >1k 0.004536822253113336 4.0895328568321485 >5k 1.9725314143971025E-4 0.6054664138244857 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7145 0.32869616421467146 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6012 0.2765740152916172 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4155 0.19114521515912666 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4084 0.18787895516483114 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3898 0.17932227405301462 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3571 0.16427907661449853 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3526 0.1622089118293816 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3231 0.1486378315713931 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3082 0.14178328594956158 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2663 0.1225077516170287 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2612 0.1201615648605629 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2467 0.11349103388629736 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2406 0.11068481051091666 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2360 0.10856864206390827 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2343 0.107786579811753 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2235 0.10281818432747247 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 2 4.600366189148656E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 3 4.600366189148656E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 4 4.600366189148656E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 5 4.600366189148656E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 6 9.200732378297312E-5 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 7 1.3801098567445968E-4 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 8 1.8401464756594624E-4 0.0 0.0 4.600366189148656E-5 4.600366189148656E-5 9 1.8401464756594624E-4 0.0 0.0 2.3001830945743282E-4 4.600366189148656E-5 10-11 1.8401464756594624E-4 0.0 0.0 2.530201404031761E-4 6.900549283722984E-5 12-13 1.8401464756594624E-4 0.0 0.0 2.7602197134891937E-4 3.9103112607763576E-4 14-15 2.7602197134891937E-4 0.0 0.0 2.7602197134891937E-4 4.1403295702337905E-4 16-17 3.2202563324040595E-4 0.0 0.0 3.450274641861492E-4 4.1403295702337905E-4 18-19 3.450274641861492E-4 0.0 0.0 4.6003661891486563E-4 4.1403295702337905E-4 20-21 3.6802929513189247E-4 0.0 0.0 5.750457736435821E-4 4.6003661891486563E-4 22-23 3.6802929513189247E-4 0.0 0.0 7.130567593180417E-4 4.6003661891486563E-4 24-25 3.6802929513189247E-4 0.0 0.0 0.0010350823925584477 4.6003661891486563E-4 26-27 3.6802929513189247E-4 0.0 0.0 0.0024151922493030444 5.060402808063522E-4 28-29 5.060402808063522E-4 0.0 0.0 0.005796461398327307 5.060402808063522E-4 30-31 5.520439426978387E-4 0.0 0.0 0.016722331097555367 5.060402808063522E-4 32-33 5.520439426978387E-4 0.0 0.0 0.03942513824100398 5.520439426978387E-4 34-35 5.750457736435821E-4 0.0 0.0 0.07441092310947953 7.130567593180417E-4 36-37 7.360585902637849E-4 0.0 0.0 0.13391665976611739 7.360585902637849E-4 38-39 8.050640831010149E-4 0.0 0.0 0.22346278763789595 7.360585902637849E-4 40-41 8.280659140467581E-4 0.0 0.0 0.31993246662434327 7.360585902637849E-4 42-43 9.660768997212178E-4 0.0 0.0 0.4190013525076596 7.360585902637849E-4 44-45 0.0011500915472871642 0.0 0.0 0.5436252725716967 7.360585902637849E-4 46-47 0.0012881025329616238 0.0 0.0 0.6895948917533836 7.590604212095282E-4 48-49 0.0012881025329616238 0.0 0.0 0.8445352250039103 7.820622521552715E-4 50-51 0.001380109856744597 0.0 0.0 1.015162806959434 8.280659140467581E-4 52-53 0.001380109856744597 0.0 0.0 1.1814660446971579 8.740695759382447E-4 54-55 0.0014261135186360834 0.0 0.0 1.3615473791713821 8.740695759382447E-4 56-57 0.0014721171805275699 0.0 0.0 1.5679428082475364 8.740695759382447E-4 58-59 0.0014721171805275699 0.0 0.0 1.7888063889885635 8.740695759382447E-4 60-61 0.0014721171805275699 0.0 0.0 2.0328558153228995 9.660768997212178E-4 62-63 0.0014721171805275699 0.0 0.0 2.2743750402532044 9.660768997212178E-4 64-65 0.0015181208424190566 0.0 0.0 2.523093838269526 9.89078730666961E-4 66-67 0.001564124504310543 0.0 0.0 2.8137219722689926 0.0010120805616127043 68-69 0.001564124504310543 0.0 0.0 3.139841931417741 0.0010120805616127043 70-71 0.0015871263352562864 0.0 0.0 3.4782218664605704 0.0010120805616127043 72-73 0.0016101281662020297 0.0 0.0 3.8357623266812038 0.0011270897163414206 74-75 0.0016331299971477729 0.0 0.0 4.18950748479579 0.001311104363907367 76-77 0.0016561318280935162 0.0 0.0 4.579066493692897 0.001380109856744597 78-79 0.0016561318280935162 0.0 0.0 5.015572239550268 0.001380109856744597 80-81 0.0017941428137679758 0.0 0.0 5.47174455086625 0.0014261135186360834 82-83 0.0018401464756594625 0.0 0.0 5.955243037345773 0.0015871263352562864 84-85 0.001886150137550949 0.0 0.0 6.4360043059427525 0.0016101281662020297 86-87 0.0019321537994424357 0.0 0.0 6.9393993761903445 0.0016331299971477729 88-89 0.0019321537994424357 0.0 0.0 7.4675444165355565 0.0016561318280935162 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4905 0.0 47.999683 1 GTATCAA 8200 0.0 38.746704 1 TAAGGTG 1405 0.0 31.128893 5 TATCAAC 10705 0.0 29.413284 2 ATCAACG 10795 0.0 29.197323 3 TCAACGC 10855 0.0 29.12353 4 CAACGCA 11070 0.0 28.557896 5 GTAAGGT 1580 0.0 28.282835 4 AGGTAAG 1440 0.0 28.075573 2 AACGCAG 11490 0.0 27.488688 6 AAGGTAA 1515 0.0 25.115946 1 TACATGG 9700 0.0 24.811384 2 GTACATG 10000 0.0 24.257296 1 ACGCAGA 13285 0.0 23.73881 7 GGTAAGG 1795 0.0 23.570984 3 CGCAGAG 13365 0.0 23.561174 8 ACATGGG 9850 0.0 23.311117 3 GTACCGT 240 5.9408194E-9 21.768766 6 TAAGGTA 685 0.0 21.514105 4 GCAGAGT 14850 0.0 20.85324 9 >>END_MODULE