FastQCFastQC Report
Thu 26 May 2016
SRR938461_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938461_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175674
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75180.34554809222337535No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58830.2703989660215639No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA40600.18660883937575207No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39400.18109330717745398No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37380.17180882797698552No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA36740.16886721080455988No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32300.14845974167085693No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29100.13375165580872872No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28850.13260258660074992No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA28320.13016655987983494No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA27970.12855786298866467No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG26050.11973301147138772No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA25370.11660754322568546No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT21900.10065846261894015No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA46100.045.6911161
GTATCAA82650.041.341551
TATCAAC107500.031.3870052
TCAACGC108600.031.025334
ATCAACG108800.030.8809383
CAACGCA111250.030.2435825
AACGCAG114500.029.2994356
TAAGGTG15600.027.112245
TAAGGTA6900.026.1718564
ACGCAGA130600.025.4650767
TACATGG95100.025.4353222
AGGTAAG14400.025.4113772
GTACATG99500.025.3362921
CGCAGAG131600.025.2715728
GTAAGGT16050.024.871634
ACATGGG101800.022.8276423
GCAGAGT141450.022.5692169
GGTAAGG17750.022.2218273
GAGTACT74000.022.05033712-13
AGAGTAC119650.021.91576210-11
Produced by FastQC (version 0.11.5)