##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938461_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175674 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.96818549102485 31.0 31.0 34.0 28.0 34.0 2 31.113837826806773 31.0 31.0 34.0 27.0 34.0 3 31.15779386066111 31.0 31.0 34.0 27.0 34.0 4 34.83919190099252 37.0 35.0 37.0 32.0 37.0 5 34.840678336919964 37.0 35.0 37.0 32.0 37.0 6 34.84579399303388 37.0 35.0 37.0 32.0 37.0 7 34.75715387507503 37.0 35.0 37.0 31.0 37.0 8 34.70247150997806 37.0 35.0 37.0 31.0 37.0 9 36.170097634112466 39.0 35.0 39.0 31.0 39.0 10-11 36.01701449757638 38.5 35.0 39.0 30.0 39.0 12-13 35.84328419606982 38.0 35.0 39.0 30.0 39.0 14-15 36.799481677861664 39.0 36.0 41.0 30.5 41.0 16-17 36.63978449896446 39.0 36.0 41.0 30.0 41.0 18-19 36.52209361328949 39.0 36.0 40.0 30.0 41.0 20-21 36.4946418902832 39.0 36.0 40.5 29.5 41.0 22-23 36.575142231786565 39.0 36.0 41.0 30.0 41.0 24-25 36.47195581691007 39.0 36.0 41.0 29.5 41.0 26-27 36.27091765586205 39.0 35.0 41.0 29.0 41.0 28-29 36.08641345164763 39.0 35.0 40.0 28.5 41.0 30-31 35.80196757418621 38.0 35.0 40.0 27.0 41.0 32-33 35.47640363400032 38.0 34.0 40.0 27.0 41.0 34-35 35.20088832242331 38.0 34.0 40.0 26.0 41.0 36-37 34.894742272969204 38.0 34.0 40.0 25.0 41.0 38-39 34.548874279878326 38.0 33.0 40.0 24.0 41.0 40-41 34.423620680304126 38.0 33.0 40.0 24.0 41.0 42-43 34.17467001030485 38.0 33.0 40.0 23.5 41.0 44-45 33.852676917589676 38.0 33.0 40.0 22.5 41.0 46-47 33.4338184397111 37.5 32.5 40.0 19.5 41.0 48-49 33.01812886489428 37.0 31.5 40.0 17.0 41.0 50-51 32.21719867038904 36.5 30.5 39.5 15.0 40.5 52-53 32.23681443083844 36.5 31.0 39.0 13.5 40.0 54-55 32.303632115840884 36.5 31.0 39.0 11.0 41.0 56-57 31.969200119135497 36.0 31.0 39.0 8.0 41.0 58-59 31.532543248666848 36.0 30.0 39.0 7.0 41.0 60-61 31.045732724663715 35.0 29.0 39.0 3.5 40.0 62-63 30.466578173016728 35.0 28.5 38.5 2.0 40.0 64-65 29.965383600668115 34.5 28.0 38.0 2.0 40.0 66-67 29.409243526373896 34.0 27.5 37.5 2.0 40.0 68-69 28.790040005993546 34.0 26.0 37.0 2.0 39.0 70-71 28.13593948358072 34.0 26.0 36.0 2.0 39.0 72-73 27.512344220687474 33.0 25.0 36.0 2.0 39.0 74-75 26.838437422150562 32.5 23.5 35.0 2.0 38.0 76-77 26.118956700314477 32.0 21.0 35.0 2.0 37.0 78-79 25.36091689288009 31.5 19.0 35.0 2.0 36.5 80-81 24.64431114220237 31.0 15.0 35.0 2.0 36.0 82-83 23.93388968200199 31.0 7.5 34.0 2.0 35.5 84-85 23.226435807938138 30.0 3.5 34.0 2.0 35.0 86-87 22.527689810146192 29.5 2.0 34.0 2.0 35.0 88-89 21.754990407570254 29.0 2.0 34.0 2.0 35.0 90-91 20.933371451789192 29.0 2.0 34.0 2.0 35.0 92-93 20.085664258524027 27.0 2.0 34.0 2.0 35.0 94-95 19.179326498363267 25.5 2.0 33.0 2.0 35.0 96-97 18.102627507613732 24.5 2.0 33.0 2.0 35.0 98-99 16.85176961254306 11.0 2.0 32.5 2.0 34.5 100-101 14.132505375345755 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5898365835835939 1101 2 0.6575863843315659 1101 3 0.8157827476574475 1101 4 0.6761765895334761 1101 5 0.8371636064790096 1101 6 0.6663336888621458 1101 7 0.6537542620412822 1101 8 0.48168849307501915 1101 9 0.6452699013148617 1101 10-11 0.7859193758162561 1101 12-13 0.9806644257510229 1101 14-15 1.068226392820364 1101 16-17 1.2740460087602372 1101 18-19 1.1813403717914213 1101 20-21 1.151790187249695 1101 22-23 1.1084990173932852 1101 24-25 1.276916547649627 1101 26-27 1.3000632126738552 1101 28-29 1.3061288323492093 1101 30-31 1.2740476335016595 1101 32-33 1.3154702539106253 1101 34-35 1.4418058546489476 1101 36-37 1.420166656426197 1101 38-39 1.6272029839624906 1101 40-41 1.670905406333624 1101 42-43 1.6591495213331626 1101 44-45 1.6177539782794312 1101 46-47 1.9439841596238736 1101 48-49 2.0528215237785687 1101 50-51 2.204255827694503 1101 52-53 2.3279972108149423 1101 54-55 2.430570226576691 1101 56-57 2.3652735717947877 1101 58-59 2.6455341533368717 1101 60-61 2.708131268441697 1101 62-63 2.6485457372964483 1101 64-65 2.5956906982106958 1101 66-67 2.556591696817545 1101 68-69 2.850484239670326 1101 70-71 2.930842032682566 1101 72-73 3.0628264519679043 1101 74-75 3.1734306691530456 1101 76-77 3.288602200595342 1101 78-79 3.53559757513991 1101 80-81 3.981968488632532 1101 82-83 4.192974715061304 1101 84-85 4.375179071446947 1101 86-87 4.542570266628246 1101 88-89 4.811404750408528 1101 90-91 4.866297159559032 1101 92-93 4.822860588367853 1101 94-95 4.916377227084517 1101 96-97 5.128768105005797 1101 98-99 5.496736109703221 1101 100-101 5.43596524274278 1102 1 0.6191346380615066 1102 2 0.5684540865661738 1102 3 0.6876546195293187 1102 4 0.611189711596964 1102 5 0.580576083704436 1102 6 0.7086411556300476 1102 7 0.6457159050820636 1102 8 0.7728949101807174 1102 9 0.8112781500569213 1102 10-11 0.8445769542580095 1102 12-13 0.8214600003595436 1102 14-15 1.0216945063604186 1102 16-17 1.0286623928730307 1102 18-19 1.238634304984629 1102 20-21 1.1536252700200293 1102 22-23 0.8520231170324877 1102 24-25 0.96934252964396 1102 26-27 0.9421523727486303 1102 28-29 0.931678328402306 1102 30-31 0.916600000874169 1102 32-33 1.0118953532134256 1102 34-35 1.1396900183604473 1102 36-37 1.2488221361608112 1102 38-39 1.2886287982660036 1102 40-41 1.4824978312353991 1102 42-43 1.539557935914246 1102 44-45 1.6307582466793846 1102 46-47 1.7733767588006444 1102 48-49 1.6177361300600808 1102 50-51 1.7638709412664468 1102 52-53 1.6463065273257058 1102 54-55 1.934812907078367 1102 56-57 1.917438789427635 1102 58-59 1.9141205712038705 1102 60-61 1.9078345688644163 1102 62-63 2.151749609205357 1102 64-65 2.2311396106380244 1102 66-67 2.2991127378997405 1102 68-69 2.362131549878786 1102 70-71 2.467374196912573 1102 72-73 2.623585052294846 1102 74-75 2.6782204023600436 1102 76-77 2.7779952421466554 1102 78-79 3.2989611442588753 1102 80-81 3.636905028461161 1102 82-83 3.9088252006959614 1102 84-85 3.878379085438329 1102 86-87 3.989879600628228 1102 88-89 4.208254323517092 1102 90-91 4.6467011503946765 1102 92-93 4.3638653649841395 1102 94-95 4.724062095920463 1102 96-97 4.912317584598441 1102 98-99 5.2947309184894 1102 100-101 5.330158511036769 1103 1 0.5525177996573696 1103 2 0.6345460278427524 1103 3 0.5469496605082611 1103 4 0.4962091475765149 1103 5 0.39849888094776986 1103 6 0.42709077215942415 1103 7 0.528350385944016 1103 8 0.588405398512478 1103 9 0.6965726025596481 1103 10-11 0.6119743368695723 1103 12-13 0.713027343182425 1103 14-15 0.8468594053805631 1103 16-17 0.9726266673395187 1103 18-19 1.1541155173639268 1103 20-21 0.9806557717181832 1103 22-23 0.878645381837913 1103 24-25 0.9442195484648437 1103 26-27 0.9972662014961386 1103 28-29 1.160038150558286 1103 30-31 1.1812756809855998 1103 32-33 1.2090322981347086 1103 34-35 1.2106121221481345 1103 36-37 1.296767422658533 1103 38-39 1.367691243281186 1103 40-41 1.319546105506717 1103 42-43 1.384382080590683 1103 44-45 1.3391730869258325 1103 46-47 1.4240976421655418 1103 48-49 1.5045121231876237 1103 50-51 1.4371217400711558 1103 52-53 1.4562494096467233 1103 54-55 1.7317815892021429 1103 56-57 1.918290453499388 1103 58-59 2.0468666851168926 1103 60-61 2.152916060991707 1103 62-63 2.100110081938059 1103 64-65 2.2473807409855056 1103 66-67 2.262963590229173 1103 68-69 2.3979794361698055 1103 70-71 2.5786638469762266 1103 72-73 2.708289402139524 1103 74-75 2.7462722060100724 1103 76-77 2.904556071809406 1103 78-79 2.975936125148184 1103 80-81 3.448391551912234 1103 82-83 3.7189839562018356 1103 84-85 4.09098275262102 1103 86-87 4.191900306710977 1103 88-89 4.433218209116134 1103 90-91 4.763898850310248 1103 92-93 4.780614832324449 1103 94-95 4.970974444014498 1103 96-97 5.0998481727937275 1103 98-99 5.207246831743863 1103 100-101 5.297560920832089 1104 1 0.5796758475287298 1104 2 0.5661336276824684 1104 3 0.5685273777290476 1104 4 0.5230581432744614 1104 5 0.34310615554238666 1104 6 0.4292400361334572 1104 7 0.537249443393641 1104 8 0.6019724363942842 1104 9 0.5998686498646038 1104 10-11 0.7975769709811757 1104 12-13 0.7449431493796865 1104 14-15 0.7720345839081375 1104 16-17 0.9458544058884257 1104 18-19 1.0150747072006894 1104 20-21 0.9392953075993731 1104 22-23 0.8852808377603765 1104 24-25 0.8511693445256654 1104 26-27 1.0771536030959155 1104 28-29 0.9882413353233019 1104 30-31 0.9928343497815533 1104 32-33 1.0141497555871481 1104 34-35 1.0888134855030103 1104 36-37 1.1896488817608244 1104 38-39 1.2831446745241237 1104 40-41 1.2412577845060966 1104 42-43 1.1613236156751512 1104 44-45 1.1987655140899918 1104 46-47 1.2945609501518973 1104 48-49 1.3227564248178467 1104 50-51 1.1813674353739216 1104 52-53 1.4624548952640453 1104 54-55 1.58755622318683 1104 56-57 1.7005194834868433 1104 58-59 1.7300229891349268 1104 60-61 1.851173970318385 1104 62-63 2.00507501097945 1104 64-65 1.9927020083579379 1104 66-67 2.0536634700161045 1104 68-69 2.2749511714096293 1104 70-71 2.520810807747754 1104 72-73 2.652012764016117 1104 74-75 2.5251601822353855 1104 76-77 2.9424831190450327 1104 78-79 3.0232225178042604 1104 80-81 3.4357771702927273 1104 82-83 3.665474451378625 1104 84-85 3.850584345591084 1104 86-87 3.8923167633279228 1104 88-89 4.477119011594191 1104 90-91 4.897721144135435 1104 92-93 4.708886878144082 1104 94-95 4.825927445757177 1104 96-97 4.922028130466504 1104 98-99 5.031210596257857 1104 100-101 4.989803358748441 1105 1 0.6575810400601725 1105 2 0.6410183251803652 1105 3 0.531323913244865 1105 4 0.5597756703586825 1105 5 0.6117761088915117 1105 6 0.6621223889447663 1105 7 0.6857661310712331 1105 8 0.5356215591238325 1105 9 0.7359609271911296 1105 10-11 0.7670153501006567 1105 12-13 0.8758332913193314 1105 14-15 1.0359941899478002 1105 16-17 1.1214832433256987 1105 18-19 1.1253223343646113 1105 20-21 1.020269897996755 1105 22-23 1.0466724379537808 1105 24-25 1.0113762377276174 1105 26-27 1.0785725579662184 1105 28-29 1.1483968971097696 1105 30-31 1.1844318938216958 1105 32-33 1.138611106894949 1105 34-35 1.1383517235438916 1105 36-37 1.3607948859688932 1105 38-39 1.233943678363488 1105 40-41 1.2559523681034435 1105 42-43 1.5182730062111887 1105 44-45 1.5407973329441873 1105 46-47 1.5399539280142562 1105 48-49 1.5147786397297907 1105 50-51 1.437266353329754 1105 52-53 1.617580530283334 1105 54-55 1.7789389480129145 1105 56-57 1.8295947011210103 1105 58-59 1.8993349715431513 1105 60-61 2.0592267103120676 1105 62-63 2.316309636392578 1105 64-65 2.2901991326947737 1105 66-67 2.3204705897386297 1105 68-69 2.444117328441809 1105 70-71 2.7436588071472308 1105 72-73 2.825665949102852 1105 74-75 2.919046334675336 1105 76-77 2.9755940898380118 1105 78-79 3.346805936321985 1105 80-81 3.6947313561764794 1105 82-83 3.7913893096708016 1105 84-85 4.026241332514267 1105 86-87 4.354642374502937 1105 88-89 4.796617663730572 1105 90-91 4.899612016857809 1105 92-93 4.823913294504781 1105 94-95 5.156476702941916 1105 96-97 5.288812951380578 1105 98-99 5.353163773175719 1105 100-101 4.8694776636769195 1106 1 0.2811223266847058 1106 2 0.304308632701094 1106 3 0.2569042429845503 1106 4 0.2692239140236836 1106 5 0.23493137357375105 1106 6 0.12825750094732769 1106 7 0.2934580397400879 1106 8 0.25193563314667955 1106 9 0.339381954796238 1106 10-11 0.09033509557689712 1106 12-13 0.08230402195123077 1106 14-15 0.35299169139592834 1106 16-17 0.061162884018372665 1106 18-19 0.3548919177375751 1106 20-21 0.3253011515089952 1106 22-23 0.39125300112219463 1106 24-25 0.4524702835661003 1106 26-27 0.4452596856690221 1106 28-29 0.27475294507505055 1106 30-31 0.4427752415353936 1106 32-33 0.7743732516726212 1106 34-35 0.6699445719794923 1106 36-37 0.6598036784507642 1106 38-39 0.5325011753159501 1106 40-41 0.8487807028921068 1106 42-43 0.41755953564481274 1106 44-45 0.6567601831953382 1106 46-47 0.8358831904659496 1106 48-49 0.9355918362335487 1106 50-51 0.18784257645302915 1106 52-53 0.7873026636489442 1106 54-55 0.5711391866289475 1106 56-57 0.6146792541914579 1106 58-59 -0.18940187647745788 1106 60-61 1.253147405065107 1106 62-63 1.6079172254197012 1106 64-65 1.6142530979571248 1106 66-67 1.7641228414612478 1106 68-69 1.1806121800416598 1106 70-71 1.5630787800003354 1106 72-73 2.038713966255539 1106 74-75 2.3716114569660505 1106 76-77 2.4727827867005594 1106 78-79 2.5520684571792707 1106 80-81 3.1164694059227322 1106 82-83 3.372523946170407 1106 84-85 3.4864691848835676 1106 86-87 3.497705650977643 1106 88-89 3.5695972084581093 1106 90-91 3.8654430231085115 1106 92-93 4.163792862575189 1106 94-95 3.928997171182644 1106 96-97 4.1349343602526005 1106 98-99 4.331857998840775 1106 100-101 3.9092761801077085 1107 1 0.41943139185692857 1107 2 0.18880073968519184 1107 3 0.2378376635121846 1107 4 0.22530737221595132 1107 5 0.26100274597344253 1107 6 0.19962948674238135 1107 7 0.2567817982560143 1107 8 0.1380137934572616 1107 9 0.15107454521204033 1107 10-11 0.22865057615270956 1107 12-13 0.34202729041310675 1107 14-15 0.3661341630232897 1107 16-17 0.5443476973094405 1107 18-19 0.5061143625444089 1107 20-21 0.6413822918780241 1107 22-23 0.5535242195522017 1107 24-25 0.48807804992951986 1107 26-27 0.5278598694392898 1107 28-29 0.6130762896939714 1107 30-31 0.6170138779930952 1107 32-33 0.6390947403773808 1107 34-35 0.7324326724405665 1107 36-37 0.7761993805442557 1107 38-39 0.7289180689361388 1107 40-41 0.9168620005419967 1107 42-43 0.9239130616334137 1107 44-45 1.0915782340715623 1107 46-47 0.8367027065375723 1107 48-49 1.0411724095636785 1107 50-51 1.1361273872607143 1107 52-53 1.2752479133529988 1107 54-55 1.4205442096830083 1107 56-57 1.426768978784395 1107 58-59 1.6295084568751896 1107 60-61 1.534323990083692 1107 62-63 1.5842888800971515 1107 64-65 1.7293317797779366 1107 66-67 1.8435014578863367 1107 68-69 2.0932335553386316 1107 70-71 1.9421119993740241 1107 72-73 2.1905386312059143 1107 74-75 2.522597773531036 1107 76-77 2.6023062670776973 1107 78-79 2.6993276312064047 1107 80-81 2.941392421079641 1107 82-83 2.991520163151776 1107 84-85 3.2174562050734643 1107 86-87 3.6140247821250497 1107 88-89 3.9484431394366446 1107 90-91 4.180727204792742 1107 92-93 4.335289845266015 1107 94-95 4.469976078991223 1107 96-97 4.578587042719093 1107 98-99 4.885957441735407 1107 100-101 4.609609574145937 1108 1 0.3666520317803119 1108 2 0.39814552712334006 1108 3 0.3042986487175199 1108 4 0.3187227746953951 1108 5 0.309689472711824 1108 6 0.4706677582043497 1108 7 0.3573652753003387 1108 8 0.35390303845033344 1108 9 0.25353356801905136 1108 10-11 0.359987683007958 1108 12-13 0.4234073540736816 1108 14-15 0.4181373272732074 1108 16-17 0.5304683065605644 1108 18-19 0.6580791473535044 1108 20-21 0.6618892760755557 1108 22-23 0.6049352606248419 1108 24-25 0.6262772912606991 1108 26-27 0.5770774922143787 1108 28-29 0.6206303104196564 1108 30-31 0.6848899339167502 1108 32-33 0.8062917814437682 1108 34-35 0.8532752909121513 1108 36-37 0.8648175485343117 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1.6738084704761569 2311 88-89 1.763293131296205 2311 90-91 2.0021634845669602 2311 92-93 2.1915378941743775 2311 94-95 2.267455193908841 2311 96-97 2.271049630303505 2311 98-99 2.2626727220129865 2311 100-101 2.557955869947113 2312 1 -0.28845271070231604 2312 2 -0.35044980292595085 2312 3 -0.28335341030224015 2312 4 -0.2998401994329498 2312 5 -0.32488593940464483 2312 6 -0.1881326234966778 2312 7 -0.20518641640849467 2312 8 -0.3851061595850567 2312 9 -0.3312831689749842 2312 10-11 -0.38124471274013416 2312 12-13 -0.42378977430199427 2312 14-15 -0.4335241841523896 2312 16-17 -0.3882019210501113 2312 18-19 -0.33223397176600145 2312 20-21 -0.4272786491191809 2312 22-23 -0.3799897473333118 2312 24-25 -0.44625807713311616 2312 26-27 -0.33010909068931227 2312 28-29 -0.23525783853386173 2312 30-31 -0.26454031261908284 2312 32-33 -0.26376420185790295 2312 34-35 -0.22580330301522622 2312 36-37 -0.2661525258726769 2312 38-39 -0.022798939632323822 2312 40-41 0.1255055036548427 2312 42-43 0.013322115560775671 2312 44-45 -0.013803736117893095 2312 46-47 -0.10778062235674213 2312 48-49 0.12812846398183098 2312 50-51 0.2162628642466089 2312 52-53 0.10341233737268851 2312 54-55 0.19138632541648803 2312 56-57 0.19870442363443885 2312 58-59 0.402359427089781 2312 60-61 0.23706435397656023 2312 62-63 0.26460392794203 2312 64-65 0.5749415573811518 2312 66-67 0.5420057749104252 2312 68-69 0.5596304679659418 2312 70-71 0.5861141832996175 2312 72-73 0.5571696035264573 2312 74-75 0.4311506258785016 2312 76-77 0.6661695126738714 2312 78-79 0.8417963488587858 2312 80-81 0.9526924324838575 2312 82-83 1.039781949299769 2312 84-85 1.2576974206390155 2312 86-87 1.4590549668624178 2312 88-89 1.5941401064617011 2312 90-91 1.5069513635860652 2312 92-93 1.7025346595357895 2312 94-95 1.9736650514057708 2312 96-97 2.202293183397572 2312 98-99 2.2526604646542374 2312 100-101 2.036320452492829 2313 1 -0.5521717316510752 2313 2 -0.6585090842057433 2313 3 -0.6268000067356887 2313 4 -0.571172871664686 2313 5 -0.526229536791412 2313 6 -0.6788480908702397 2313 7 -0.5073329345217417 2313 8 -0.5079374137656245 2313 9 -0.6515541141648029 2313 10-11 -0.509013047676909 2313 12-13 -0.5836161014881114 2313 14-15 -0.6025386356495943 2313 16-17 -0.4463848857091932 2313 18-19 -0.38547918324818653 2313 20-21 -0.539154657435013 2313 22-23 -0.7075383856225983 2313 24-25 -0.5732962472612684 2313 26-27 -0.6177249340637019 2313 28-29 -0.5305802112980018 2313 30-31 -0.45760859677128707 2313 32-33 -0.5120805024951736 2313 34-35 -0.3240601028273531 2313 36-37 -0.39262233241892375 2313 38-39 -0.43566507059263415 2313 40-41 -0.3736869191054595 2313 42-43 -0.2260587228368678 2313 44-45 -0.2222286797648323 2313 46-47 -0.11113566535215824 2313 48-49 -0.2052582987782401 2313 50-51 -0.13234464497774212 2313 52-53 -0.1246646890889167 2313 54-55 -0.23449913074613704 2313 56-57 -0.020510602547098244 2313 58-59 0.10801542641870654 2313 60-61 0.07221605292306066 2313 62-63 0.1999711040962424 2313 64-65 0.23753699699046038 2313 66-67 0.3155200319939979 2313 68-69 0.4071386534269621 2313 70-71 0.5841547494307626 2313 72-73 0.8364679392684593 2313 74-75 0.7355476519635502 2313 76-77 0.7107357063542246 2313 78-79 0.9742697794163035 2313 80-81 1.1678331776974424 2313 82-83 1.2565133056378492 2313 84-85 1.4313483808473428 2313 86-87 1.6431349196988343 2313 88-89 1.6992865285868959 2313 90-91 1.9664223836835433 2313 92-93 1.8919734690015204 2313 94-95 2.170125445743519 2313 96-97 2.1159027594749915 2313 98-99 2.276955276071334 2313 100-101 2.1626452683294115 2314 1 -0.5547093587913423 2314 2 -0.5194250234368312 2314 3 -0.5921014035895347 2314 4 -0.5221930678035207 2314 5 -0.4555634347359643 2314 6 -0.49024799901132354 2314 7 -0.42447415625844087 2314 8 -0.41533153025810776 2314 9 -0.4246476389662135 2314 10-11 -0.4981418707617067 2314 12-13 -0.5458288492301833 2314 14-15 -0.6817595510759347 2314 16-17 -0.5620769788397979 2314 18-19 -0.5651509766767546 2314 20-21 -0.583775847912058 2314 22-23 -0.7129702753945253 2314 24-25 -0.5706278510343878 2314 26-27 -0.6373490917085931 2314 28-29 -0.6868482834871372 2314 30-31 -0.6538980917478199 2314 32-33 -0.6393461940458209 2314 34-35 -0.724794941257251 2314 36-37 -0.6757321445881601 2314 38-39 -0.5585639294576978 2314 40-41 -0.45231308587640484 2314 42-43 -0.4020683242960814 2314 44-45 -0.2921215921296536 2314 46-47 -0.21230441714602222 2314 48-49 -0.23121781181421852 2314 50-51 -0.2716404349528929 2314 52-53 -0.18157726958464337 2314 54-55 -0.2002894116522711 2314 56-57 -0.28510627373690056 2314 58-59 0.07022981802107964 2314 60-61 0.07294255698126051 2314 62-63 0.22156016795397093 2314 64-65 0.34199725025672834 2314 66-67 0.34541527535271754 2314 68-69 0.5643164379043846 2314 70-71 0.6315760093444744 2314 72-73 0.6145752379752984 2314 74-75 0.7274542701779829 2314 76-77 0.799741042653519 2314 78-79 1.0185344987879823 2314 80-81 1.3607831704808966 2314 82-83 1.7033049177420523 2314 84-85 1.8342464572349044 2314 86-87 1.9690584979487227 2314 88-89 1.9867168165813567 2314 90-91 1.9876031948365416 2314 92-93 2.1211067173582343 2314 94-95 2.045495355406821 2314 96-97 1.9448551503218496 2314 98-99 2.1895122645981786 2314 100-101 2.0430185955782694 2315 1 -0.6833032798077632 2315 2 -0.6661444244600396 2315 3 -0.6406773576483431 2315 4 -0.5425533388654102 2315 5 -0.5086176057322618 2315 6 -0.560187819152759 2315 7 -0.5916636372792539 2315 8 -0.5451807596567946 2315 9 -0.756372124762251 2315 10-11 -0.5658504841159697 2315 12-13 -0.6290755899087301 2315 14-15 -0.5575110661622844 2315 16-17 -0.5449759477762512 2315 18-19 -0.5292788086149969 2315 20-21 -0.5150394457285259 2315 22-23 -0.6505557072352772 2315 24-25 -0.6875443085916046 2315 26-27 -0.7904231553919132 2315 28-29 -0.7344945273209831 2315 30-31 -0.6978799103858151 2315 32-33 -0.6154485763783839 2315 34-35 -0.6365087198674502 2315 36-37 -0.658237414276627 2315 38-39 -0.733632527458262 2315 40-41 -0.6532740281087612 2315 42-43 -0.5754300776156924 2315 44-45 -0.4214485773986425 2315 46-47 -0.4523526157744229 2315 48-49 -0.42465863408438054 2315 50-51 -0.3138763078052236 2315 52-53 -0.24875378207239862 2315 54-55 -0.31388871069979984 2315 56-57 5.997146298710732E-4 2315 58-59 0.13325069768514552 2315 60-61 0.2527441807849726 2315 62-63 0.22630533139169628 2315 64-65 0.12177477800466363 2315 66-67 0.3052701711531647 2315 68-69 0.46818140289097343 2315 70-71 0.7175894790233599 2315 72-73 0.791648011973848 2315 74-75 0.9210671373088424 2315 76-77 0.9065004180394389 2315 78-79 1.2002454784107677 2315 80-81 1.358020433051653 2315 82-83 1.331296274442856 2315 84-85 1.5622515970751145 2315 86-87 1.603441826280374 2315 88-89 1.733091736872126 2315 90-91 1.7473668221417604 2315 92-93 1.7626338175193013 2315 94-95 1.896737809300916 2315 96-97 1.99524832822177 2315 98-99 2.07333880949097 2315 100-101 2.349110026343082 2316 1 -1.0765923001209927 2316 2 -0.9731799410124573 2316 3 -0.9035227504822814 2316 4 -0.7319554178234924 2316 5 -0.7442675693740242 2316 6 -0.6548190387520165 2316 7 -0.7330845572239824 2316 8 -0.6640743037624475 2316 9 -0.6263318452215358 2316 10-11 -0.7723208447398306 2316 12-13 -0.7512716813014393 2316 14-15 -1.3806371349129307 2316 16-17 -1.0969016788464785 2316 18-19 -0.8868667645015549 2316 20-21 -0.6855174071755599 2316 22-23 -1.0035909716324483 2316 24-25 -1.1035454679029968 2316 26-27 -0.770230224813794 2316 28-29 -0.9375664848276273 2316 30-31 -0.7407376799236189 2316 32-33 -0.8714130275112453 2316 34-35 -0.7481985936910434 2316 36-37 -0.5909856335766861 2316 38-39 -0.8043357040618702 2316 40-41 -0.8424490668822315 2316 42-43 -0.6569140631621337 2316 44-45 -0.7195139974539586 2316 46-47 -0.4332633248547424 2316 48-49 -0.4002001609229282 2316 50-51 -0.41161769329618636 2316 52-53 -0.16732831462663 2316 54-55 -0.28733162273461943 2316 56-57 0.11708572863637556 2316 58-59 0.09182607745422544 2316 60-61 -0.1285669628864241 2316 62-63 0.035787222676621155 2316 64-65 0.19471076757421102 2316 66-67 0.35521700256125754 2316 68-69 0.4411871453667935 2316 70-71 0.17627377445621661 2316 72-73 0.28016163714758235 2316 74-75 0.6534451103255527 2316 76-77 0.7924447888409354 2316 78-79 0.7048092205286132 2316 80-81 1.1514472680964154 2316 82-83 1.2792895054306257 2316 84-85 1.453153011907169 2316 86-87 1.6987375904907651 2316 88-89 1.6671165549092954 2316 90-91 1.8596304914761461 2316 92-93 2.1177712965461275 2316 94-95 2.08190029790914 2316 96-97 1.9014655792239061 2316 98-99 1.9902743329249155 2316 100-101 1.984403289093036 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7339.0 3 2732.0 4 2992.0 5 3469.0 6 4134.0 7 5155.0 8 6663.0 9 8491.0 10 10535.0 11 13021.0 12 15964.0 13 18337.0 14 21043.0 15 24651.0 16 28472.0 17 32512.0 18 31159.0 19 29699.0 20 31433.0 21 33996.0 22 37695.0 23 42319.0 24 46676.0 25 52102.0 26 57867.0 27 65347.0 28 72889.0 29 82100.0 30 93061.0 31 105876.0 32 122035.0 33 142102.0 34 168585.0 35 198595.0 36 225647.0 37 215646.0 38 107436.0 39 7899.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.94614306991693 18.193216899621675 14.330778134728067 23.52986189573333 2 15.757199292301014 19.78145563823487 39.509852243928094 24.951492825536015 3 19.240710043935174 23.788954821029108 29.63548288459372 27.334852250441994 4 13.050699457456627 16.038849397886754 36.077823867218264 34.832627277438355 5 13.903433940224513 37.446504205622524 33.6009234434138 15.049138410739168 6 33.00628105923548 35.33484419680345 16.90459393858009 14.75428080538098 7 29.37255810660333 32.71210737756396 19.788612144801924 18.126722371030787 8 27.53065735850167 33.68525541055481 19.4086182671512 19.375468963792315 9 27.237657159252255 16.201794686932146 19.50282868262107 37.057719471194524 10-11 25.53573977824894 26.08392204804905 26.730218967161708 21.650119206540303 12-13 26.632630729081413 23.469072477788057 26.994775986272824 22.903520806857703 14-15 23.120712408788656 24.803761850894638 25.491546478678334 26.583979261638373 16-17 22.550933084593524 27.978056588459665 26.49963923380109 22.971371093145716 18-19 22.819561627410884 26.179443492268987 28.782361194886203 22.21863368543392 20-21 23.212592661392442 25.39699216591091 29.053927298108285 22.33648787458836 22-23 22.915407566760358 25.456580564731162 29.035684835789617 22.59232703271886 24-25 22.756357711718127 25.69823140845132 29.074188229608104 22.471222650222444 26-27 22.062960303616165 26.04908357146749 28.884874578077767 23.00308154683857 28-29 22.401190321009178 26.505839069868596 28.671940071971935 22.421030537150287 30-31 22.380772294595225 25.97326522237408 28.43795009615462 23.208012386876074 32-33 21.985045400354853 26.04080123844646 28.503487153692696 23.47066620750599 34-35 23.1019948390508 26.132334093874103 28.25427732343289 22.511393743642206 36-37 23.255001874704455 25.501750682008517 28.273439725205797 22.96980771808123 38-39 22.54388233225416 25.945655209507752 28.29193056977788 23.21853188846021 40-41 22.978689170816473 25.857162394655457 28.439125133171135 22.725023301356938 42-43 22.68658507236673 25.890801367827372 28.118152444668475 23.304461115137425 44-45 22.58747938389802 26.172750523561657 28.40016290627741 22.83960718626292 46-47 23.219163897533214 26.499785433817884 27.5281631043164 22.752887564332504 48-49 22.81075920098043 26.684567102731215 27.337652606503916 23.16702108978443 50-51 23.0710925308371 26.801697965337425 27.421519665187343 22.705689838638136 52-53 23.453040026310205 26.321261694861807 27.453602634514034 22.772095644313943 54-55 22.645318077643324 26.736467025627686 27.67186783223276 22.94634706449623 56-57 22.821762244859496 26.837521821907494 27.331209441588992 23.009506491644014 58-59 22.726626071148264 26.16914748262203 27.499859229543176 23.604367216686526 60-61 22.247641216186246 26.689805765331204 27.821847326535476 23.240705691947074 62-63 22.56270335080529 26.62642148777235 27.94595375994878 22.864921401473584 64-65 22.24240911531305 26.7310701336334 27.60461483867646 23.421905912377085 66-67 22.111975105918205 26.738822426276677 27.64750697215848 23.501695495646636 68-69 22.94160314185798 26.215980672278178 27.759927704906577 23.082488480957267 70-71 22.727234071797092 26.320329485065812 27.321136465133105 23.631299978003987 72-73 22.595510837229753 26.513007180996368 27.399168288786242 23.49231369298764 74-75 22.54974089251021 27.008108902601002 27.257138010851556 23.18501219403723 76-77 22.756500348543852 26.44575598161233 27.465990830986915 23.331752838856907 78-79 22.761871075849538 26.787266027610023 27.30423862833798 23.146624268202462 80-81 23.071718351966272 26.628057059908862 27.03227891215874 23.26794567596613 82-83 22.886121009528225 26.69053778743716 27.234590646823253 23.188750556211364 84-85 22.698336929684874 26.892083483771835 27.17134177045295 23.238237816090344 86-87 23.097056763968645 26.851108140350732 26.717263051792116 23.334572043888503 88-89 22.89696758787311 26.863787514397565 26.58472018449107 23.654524713238263 90-91 22.685210195109338 26.79081635585184 26.83649320421827 23.687480244820552 92-93 22.9729493675192 26.340924573940818 27.060515711578827 23.62561034696116 94-95 22.621393858900255 26.01738312523877 27.410212241283954 23.95101077457702 96-97 23.045588717310785 25.58273651827773 27.392489393402368 23.979185371009105 98-99 22.744592298668394 25.95721921692219 26.84141791730869 24.45677056710073 100-101 23.152205170204493 25.35111794112031 27.340935808393702 24.155741080281494 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 464.0 1 321.5 2 249.5 3 337.0 4 325.0 5 335.5 6 561.0 7 1144.5 8 1862.5 9 2173.5 10 1931.5 11 1632.0 12 1623.5 13 1750.0 14 1909.0 15 2101.0 16 2318.0 17 2529.5 18 2814.0 19 3110.5 20 3507.0 21 4159.0 22 4962.0 23 5954.0 24 7292.5 25 9093.5 26 11628.5 27 14739.0 28 17396.5 29 19912.5 30 22948.5 31 28005.0 32 35650.0 33 38180.5 34 37568.5 35 39858.0 36 43568.5 37 47839.0 38 51554.0 39 54640.5 40 57604.5 41 61383.0 42 63535.0 43 65476.0 44 68374.0 45 72829.5 46 75357.5 47 77267.0 48 79909.5 49 78843.0 50 77283.5 51 76492.0 52 75938.5 53 77043.0 54 79001.5 55 76788.5 56 71654.5 57 66755.0 58 63154.0 59 56804.0 60 46345.5 61 38740.5 62 33715.5 63 26362.5 64 19105.0 65 14609.5 66 11099.0 67 8635.0 68 6813.5 69 5373.0 70 4215.5 71 3009.0 72 2209.0 73 1765.5 74 1321.0 75 938.0 76 696.5 77 507.0 78 350.5 79 233.5 80 140.5 81 91.0 82 56.0 83 32.0 84 22.0 85 18.0 86 12.5 87 9.5 88 11.0 89 7.5 90 4.0 91 3.5 92 2.5 93 3.0 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2928287969613095 2 0.03392052301953326 3 0.04040127335253351 4 0.04214785854866124 5 0.05533917305625752 6 0.055155321982980905 7 0.02849691635787347 8 0.030611203700554403 9 0.037229842338512106 10-11 0.030680147853033128 12-13 0.03300126765315024 14-15 0.026405610399352106 16-17 0.02033852498122421 18-19 0.02472796935570311 20-21 0.037689470021703614 22-23 0.031001887231267188 24-25 0.0501223988520339 26-27 0.0438714623606294 28-29 0.03670127050284188 30-31 0.036379531124607824 32-33 0.03176027290853317 34-35 0.031553440451096995 36-37 0.030542259548075677 38-39 0.018224237638543275 40-41 0.017075168430564506 42-43 0.01836212594350073 44-45 0.008732925980638644 46-47 0.016293801369138942 48-49 0.014156532642298434 50-51 0.005929197113170448 52-53 0.004251556069521445 54-55 0.006250936491404503 56-57 0.004963978978468281 58-59 0.006733545558755586 60-61 0.006227955107244928 62-63 0.004251556069521445 64-65 0.007813670614255627 66-67 0.0025968964100320175 68-69 0.00671056417459601 70-71 0.012846593745202636 72-73 0.012846593745202636 74-75 0.010364604255968494 76-77 0.008939758438074822 78-79 0.005676401887415118 80-81 0.006250936491404503 82-83 0.011122989933234483 84-85 0.0041136677645639925 86-87 0.0011260878238191934 88-89 0.018040386565266672 90-91 0.02810623282716069 92-93 0.012501872982809007 94-95 0.021188836195128496 96-97 0.017327963656319834 98-99 0.011858394226340895 100-101 0.10382989363296156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.48250899476967 #Duplication Level Percentage of deduplicated Percentage of total 1 73.48905385002959 36.36422768151202 2 14.000292916030396 13.855392402937683 3 5.191594959916042 7.706794329037393 4 2.422024856259861 4.793914669417372 5 1.3390321312382518 3.3129334739141196 6 0.8099835382833616 2.4048010631233083 7 0.5266757985263855 1.8242867956536557 8 0.3799081382824804 1.503904629579923 9 0.2739852260889077 1.220172877294054 >10 1.4058810415727045 12.63199628754911 >50 0.09842217024162825 3.352484429230087 >100 0.0550912084848358 5.199420034722178 >500 0.003746123277023207 1.3164342023936133 >1k 0.004120735604725521 3.892209392925583 >5k 1.8730616385116003E-4 0.6210277307099231 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7518 0.34554809222337535 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5883 0.2703989660215639 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4060 0.18660883937575207 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3940 0.18109330717745398 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3738 0.17180882797698552 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3674 0.16886721080455988 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3230 0.14845974167085693 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2910 0.13375165580872872 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2885 0.13260258660074992 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2832 0.13016655987983494 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2797 0.12855786298866467 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2605 0.11973301147138772 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2537 0.11660754322568546 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2190 0.10065846261894015 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.596276831915076E-5 2 4.596276831915076E-5 0.0 0.0 0.0 4.596276831915076E-5 3 4.596276831915076E-5 0.0 0.0 0.0 4.596276831915076E-5 4 4.596276831915076E-5 0.0 0.0 0.0 4.596276831915076E-5 5 9.192553663830152E-5 0.0 0.0 0.0 4.596276831915076E-5 6 9.192553663830152E-5 0.0 0.0 0.0 4.596276831915076E-5 7 1.3788830495745227E-4 0.0 0.0 0.0 4.596276831915076E-5 8 1.3788830495745227E-4 0.0 0.0 0.0 4.596276831915076E-5 9 1.3788830495745227E-4 0.0 0.0 1.8385107327660303E-4 4.596276831915076E-5 10-11 1.3788830495745227E-4 0.0 0.0 2.7577660991490453E-4 4.596276831915076E-5 12-13 1.3788830495745227E-4 0.0 0.0 3.2173937823405527E-4 2.298138415957538E-4 14-15 1.8385107327660303E-4 0.0 0.0 3.6770214655320606E-4 3.217393782340553E-4 16-17 1.8385107327660303E-4 0.0 0.0 4.136649148723568E-4 4.136649148723568E-4 18-19 1.8385107327660303E-4 0.0 0.0 4.5962768319150754E-4 4.5962768319150754E-4 20-21 2.2981384159575377E-4 0.0 0.0 5.055904515106583E-4 5.515532198298091E-4 22-23 3.2173937823405527E-4 0.0 0.0 8.273298297447136E-4 6.434787564681105E-4 24-25 3.6770214655320606E-4 0.0 0.0 0.0013788830495745225 6.434787564681105E-4 26-27 3.6770214655320606E-4 0.0 0.0 0.0023900639525958394 7.124229089468367E-4 28-29 6.204973723085351E-4 0.0 0.0 0.005837271576532146 7.354042931064121E-4 30-31 6.894415247872613E-4 0.0 0.0 0.01654659659489427 7.354042931064121E-4 32-33 6.894415247872613E-4 0.0 0.0 0.03911431583959729 8.043484455851382E-4 34-35 7.813670614255628E-4 0.0 0.0 0.07312676439576885 9.192553663830151E-4 36-37 8.273298297447136E-4 0.0 0.0 0.13172929400268607 9.192553663830151E-4 38-39 8.962739822234397E-4 0.0 0.0 0.2206672507002428 9.192553663830151E-4 40-41 9.422367505425905E-4 0.0 0.0 0.31447726083962946 9.192553663830151E-4 42-43 9.881995188617413E-4 0.0 0.0 0.4113667764563993 9.192553663830151E-4 44-45 0.0010571436713404673 0.0 0.0 0.5330761869655104 0.0010111809030213166 46-47 0.0013559016654149472 0.0 0.0 0.681880649398761 0.0011260878238191934 48-49 0.0014708085862128243 0.0 0.0 0.8330751757846074 0.001149069207978769 50-51 0.0014708085862128243 0.0 0.0 1.0021722004307632 0.0011950319762979197 52-53 0.0014708085862128243 0.0 0.0 1.167086613159876 0.0011950319762979197 54-55 0.0014708085862128243 0.0 0.0 1.3463873723728832 0.0011950319762979197 56-57 0.0014708085862128243 0.0 0.0 1.5555639309933382 0.0011950319762979197 58-59 0.0014708085862128243 0.0 0.0 1.7745765220340917 0.001218013360457495 60-61 0.0014708085862128243 0.0 0.0 2.016961180765133 0.0012409947446170703 62-63 0.0014708085862128243 0.0 0.0 2.2516470758027167 0.0012409947446170703 64-65 0.0015167713545319748 0.0 0.0 2.500052857183567 0.0012409947446170703 66-67 0.0015167713545319748 0.0 0.0 2.7911350689487486 0.0012409947446170703 68-69 0.0015167713545319748 0.0 0.0 3.115632213281953 0.001286957512936221 70-71 0.0015627341228511256 0.0 0.0 3.448701413906679 0.0013099388970957966 72-73 0.0016086968911702764 0.0 0.0 3.7940656550567775 0.0013559016654149472 74-75 0.001631678275329852 0.0 0.0 4.1468069205221 0.001470808586212824 76-77 0.0016546596594894272 0.0 0.0 4.528918394943361 0.0015627341228511256 78-79 0.0016546596594894272 0.0 0.0 4.96083052883842 0.0015627341228511256 80-81 0.0019763990377234825 0.0 0.0 5.4051985729479695 0.001631678275329852 82-83 0.001999380421883058 0.0 0.0 5.859839295776849 0.0017465851961277288 84-85 0.002022361806042633 0.0 0.0 6.323534683964601 0.0017465851961277288 86-87 0.002068324574361784 2.298138415957538E-5 0.0 6.809361145098025 0.001769566580287304 88-89 0.002068324574361784 4.596276831915076E-5 0.0 7.3204211660386616 0.0017925479644468794 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4610 0.0 45.691116 1 GTATCAA 8265 0.0 41.34155 1 TATCAAC 10750 0.0 31.387005 2 TCAACGC 10860 0.0 31.02533 4 ATCAACG 10880 0.0 30.880938 3 CAACGCA 11125 0.0 30.243582 5 AACGCAG 11450 0.0 29.299435 6 TAAGGTG 1560 0.0 27.11224 5 TAAGGTA 690 0.0 26.171856 4 ACGCAGA 13060 0.0 25.465076 7 TACATGG 9510 0.0 25.435322 2 AGGTAAG 1440 0.0 25.411377 2 GTACATG 9950 0.0 25.336292 1 CGCAGAG 13160 0.0 25.271572 8 GTAAGGT 1605 0.0 24.87163 4 ACATGGG 10180 0.0 22.827642 3 GCAGAGT 14145 0.0 22.569216 9 GGTAAGG 1775 0.0 22.221827 3 GAGTACT 7400 0.0 22.050337 12-13 AGAGTAC 11965 0.0 21.915762 10-11 >>END_MODULE