FastQCFastQC Report
Thu 26 May 2016
SRR938461_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938461_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2175674
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69840.3210039739409489No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60280.2770635674278407No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42490.19529580258807155No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA41130.18904486609666707No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA40000.18385107327660302No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC34870.16027217312887868No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA34630.15916906668921904No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33230.15273427912453796No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG31830.14629949155985686No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA27560.1266733894875795No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG26330.12101996898432393No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24320.11178145255217463No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA24170.11109201102738739No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT23530.10815039385496172No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA22890.10520877668253607No Hit
ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT21760.10001498386247205No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47900.050.554081
GTATCAA83700.040.4695631
ATCAACG110050.030.7619633
TCAACGC110600.030.6089864
TAAGGTG16200.030.5240695
TATCAAC111300.030.433982
CAACGCA112700.029.9964455
AACGCAG116100.029.0449686
AGGTAAG14800.028.9304222
GTAAGGT17550.027.092374
ACGCAGA132600.025.3949577
GGTAAGG17850.025.3051833
CGCAGAG134450.025.01028
TACATGG97500.023.5676612
GTACATG100700.023.0549561
TAAGGTA8250.023.0531434
AAGGTAA17150.023.024381
AAGGTGT22650.022.6464586
GCAGAGT149100.022.2661069
GAGTACT78900.022.12203612-13
Produced by FastQC (version 0.11.5)