##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938461_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2175674 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.755219761784165 31.0 31.0 34.0 30.0 34.0 2 31.981929737635326 31.0 31.0 34.0 30.0 34.0 3 32.04720514194682 33.0 31.0 34.0 30.0 34.0 4 35.74248991347049 37.0 35.0 37.0 33.0 37.0 5 35.6084638599349 37.0 35.0 37.0 33.0 37.0 6 35.64431665773457 37.0 35.0 37.0 33.0 37.0 7 35.56986524635584 37.0 35.0 37.0 33.0 37.0 8 35.55155413908518 37.0 35.0 37.0 33.0 37.0 9 37.10805203353076 39.0 37.0 39.0 33.0 39.0 10-11 37.038467160061664 39.0 37.0 39.0 33.0 39.0 12-13 36.93386807950088 39.0 37.0 39.0 33.0 39.0 14-15 37.96291241242943 40.0 37.0 41.0 33.0 41.0 16-17 37.88726550944673 40.0 37.0 41.0 33.0 41.0 18-19 37.89810881593474 40.0 37.0 41.0 33.0 41.0 20-21 37.879082298175184 40.0 37.0 41.0 32.5 41.0 22-23 37.909064731205135 40.0 37.0 41.0 32.5 41.0 24-25 37.83110452209293 40.0 37.0 41.0 32.0 41.0 26-27 37.64752715710166 40.0 37.0 41.0 32.0 41.0 28-29 37.51987085381357 39.5 36.5 41.0 32.0 41.0 30-31 37.24934503055145 39.0 36.0 41.0 31.0 41.0 32-33 37.01038528750171 39.0 36.0 40.5 31.0 41.0 34-35 36.832363672131024 39.0 35.0 40.5 30.0 41.0 36-37 36.81842178561678 39.0 35.0 41.0 30.0 41.0 38-39 36.66003454561667 39.0 35.0 41.0 30.0 41.0 40-41 36.458677632770346 39.0 35.0 40.0 30.0 41.0 42-43 36.21336353700049 38.5 35.0 40.0 30.0 41.0 44-45 35.913804641688046 38.0 35.0 40.0 29.0 41.0 46-47 35.53130064522534 38.0 34.5 40.0 27.5 41.0 48-49 35.10064513341612 38.0 34.0 40.0 26.5 41.0 50-51 34.70025035919903 38.0 33.5 40.0 25.5 41.0 52-53 34.159588476950134 37.5 33.0 40.0 24.0 41.0 54-55 33.82013160059825 37.0 33.0 40.0 23.0 41.0 56-57 33.442809216822 37.0 32.0 39.5 23.0 41.0 58-59 32.97991610875526 36.0 31.5 39.0 21.5 40.5 60-61 32.55436269404332 36.0 31.0 39.0 20.0 40.0 62-63 32.072313683024205 35.0 31.0 38.5 18.5 40.0 64-65 31.55953764212837 35.0 30.0 38.0 17.0 40.0 66-67 31.068209437627146 34.5 29.5 38.0 13.0 40.0 68-69 30.502919095415947 34.0 29.0 37.0 8.0 39.0 70-71 29.931092158108246 34.0 28.5 36.5 7.5 39.0 72-73 29.361513949240557 34.0 28.0 36.0 6.0 39.0 74-75 28.759059721263387 33.0 26.5 35.5 2.0 38.0 76-77 27.516193832348044 31.5 26.0 34.0 2.0 36.0 78-79 28.14421599927195 33.0 26.0 35.0 2.0 37.0 80-81 27.88037316252343 33.0 26.0 35.0 2.0 36.0 82-83 27.40520270959712 33.0 26.0 35.0 2.0 36.0 84-85 26.83514510905586 32.0 25.0 35.0 2.0 35.5 86-87 26.215504023121113 32.0 24.0 34.5 2.0 35.0 88-89 25.466415005189198 31.0 21.0 34.0 2.0 35.0 90-91 24.931990500415043 31.0 19.5 34.0 2.0 35.0 92-93 24.16839333466319 31.0 15.0 34.0 2.0 35.0 94-95 23.39376947097773 30.5 4.5 34.0 2.0 35.0 96-97 22.420821317899648 30.0 2.0 34.0 2.0 35.0 98-99 21.247675892619945 29.5 2.0 34.0 2.0 35.0 100-101 18.114167379855623 23.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.34489824263533464 1101 2 0.3669872516237227 1101 3 0.3317761235214789 1101 4 0.22905486289323562 1101 5 0.24524515604265673 1101 6 0.3055176805376263 1101 7 0.3456386036807899 1101 8 0.2830658377961939 1101 9 0.43364587816660105 1101 10-11 0.4209491488279369 1101 12-13 0.4033048970644231 1101 14-15 0.6321930014653958 1101 16-17 0.5437574992575733 1101 18-19 0.5995449339060102 1101 20-21 0.5955731769687631 1101 22-23 0.6413812961383627 1101 24-25 0.5771932581772461 1101 26-27 0.5832306622762644 1101 28-29 0.5105263111630336 1101 30-31 0.5571932296163098 1101 32-33 0.5123501316189234 1101 34-35 0.5648685358698771 1101 36-37 0.6777245816405824 1101 38-39 0.8773038641659383 1101 40-41 1.056859631078737 1101 42-43 1.0425746372789533 1101 44-45 0.9014370071760993 1101 46-47 0.9195520398354233 1101 48-49 0.8946893166543575 1101 50-51 1.0188078936720544 1101 52-53 1.0398317085307411 1101 54-55 0.9734323260225395 1101 56-57 1.1775821847367567 1101 58-59 1.2039215888226593 1101 60-61 1.231124579370011 1101 62-63 1.2369526988291994 1101 64-65 1.2439406969298226 1101 66-67 1.5201657160594202 1101 68-69 1.4406547552935471 1101 70-71 1.5695999021943088 1101 72-73 1.6615499384262655 1101 74-75 1.6845876084500127 1101 76-77 1.5823323787699444 1101 78-79 1.853438774039585 1101 80-81 1.9528126132861416 1101 82-83 2.090258320444587 1101 84-85 2.365631345024365 1101 86-87 2.468438728789099 1101 88-89 2.4946562439046396 1101 90-91 2.8973985667899136 1101 92-93 3.016611350660913 1101 94-95 3.1690087421859765 1101 96-97 3.569428168867695 1101 98-99 3.793793807384535 1101 100-101 4.500226084192718 1102 1 0.3169175383956322 1102 2 0.27442192164832235 1102 3 0.2918682814553648 1102 4 0.20736719372354884 1102 5 0.17801811399547063 1102 6 0.22089503116677633 1102 7 0.21739422363784655 1102 8 0.36209965064295346 1102 9 0.2937958534244913 1102 10-11 0.30202781228070563 1102 12-13 0.37964917556698197 1102 14-15 0.5421903575059233 1102 16-17 0.40873810164824675 1102 18-19 0.4782594654982333 1102 20-21 0.5687333875390479 1102 22-23 0.49211820982491616 1102 24-25 0.4290921766229232 1102 26-27 0.4110919253893215 1102 28-29 0.45774434111488205 1102 30-31 0.4473091934732807 1102 32-33 0.35255853117983804 1102 34-35 0.44272763854912256 1102 36-37 0.5172004912603754 1102 38-39 0.7019651132876206 1102 40-41 0.5771440470466018 1102 42-43 0.5457628203232403 1102 44-45 0.5315925995619111 1102 46-47 0.5783454684130263 1102 48-49 0.6868172616599892 1102 50-51 0.7050838407393698 1102 52-53 0.7231257425441981 1102 54-55 0.7269221982174443 1102 56-57 0.7984085609588121 1102 58-59 1.0325786117242828 1102 60-61 1.086185447020803 1102 62-63 1.2175512315860075 1102 64-65 1.1714097898665408 1102 66-67 1.040055338467031 1102 68-69 1.3263572614996555 1102 70-71 1.3016132619055085 1102 72-73 1.3557906848252834 1102 74-75 1.457477919110108 1102 76-77 1.4179948655115702 1102 78-79 1.7470402377849332 1102 80-81 1.919361639187688 1102 82-83 1.854626491287572 1102 84-85 2.1889173751449285 1102 86-87 2.262816096907475 1102 88-89 2.2267765965285875 1102 90-91 2.249175317847236 1102 92-93 2.6959003518923588 1102 94-95 2.9837618303203612 1102 96-97 3.334326417578893 1102 98-99 3.590885349080395 1102 100-101 3.8721298481828086 1103 1 0.2953398307027122 1103 2 0.2808382345638911 1103 3 0.26781986080428766 1103 4 0.19857331860766436 1103 5 0.10192651279308507 1103 6 0.21253142383714163 1103 7 0.20637655900127783 1103 8 0.2470880363698953 1103 9 0.4050437335054582 1103 10-11 0.4120684289682899 1103 12-13 0.36275598991456093 1103 14-15 0.485948739225897 1103 16-17 0.5502307760065008 1103 18-19 0.46987774552123796 1103 20-21 0.40705102570897367 1103 22-23 0.4072142512134249 1103 24-25 0.4116959029489493 1103 26-27 0.5014281996251526 1103 28-29 0.4927919441093067 1103 30-31 0.5653825844417995 1103 32-33 0.6010341116347462 1103 34-35 0.6885979549511987 1103 36-37 0.7157003204839825 1103 38-39 0.7311009699714859 1103 40-41 0.7850524724587302 1103 42-43 0.8114498515198747 1103 44-45 0.8233855002912946 1103 46-47 0.8378557172142678 1103 48-49 0.9787099667727901 1103 50-51 0.7990766518665993 1103 52-53 1.1651046490725818 1103 54-55 1.126304963552947 1103 56-57 0.8991947237833884 1103 58-59 1.1253682255626458 1103 60-61 1.2840871892356986 1103 62-63 1.3310665193996627 1103 64-65 1.2748185900995672 1103 66-67 1.204532592678408 1103 68-69 1.4247603174359433 1103 70-71 1.3811869271512585 1103 72-73 1.467030432697232 1103 74-75 1.4936477669490849 1103 76-77 1.4479779505168082 1103 78-79 1.7407322311076534 1103 80-81 2.141763878945909 1103 82-83 2.11509116571089 1103 84-85 2.306946408609708 1103 86-87 2.451798654058422 1103 88-89 2.6350711540107383 1103 90-91 2.7976584390777752 1103 92-93 2.945361408221956 1103 94-95 3.1425299041549053 1103 96-97 3.4171459431606053 1103 98-99 3.8477844194949746 1103 100-101 4.337019405391423 1104 1 0.32297873085300566 1104 2 0.3219818921199824 1104 3 0.2981645538614188 1104 4 0.12877523661130397 1104 5 0.107365361003815 1104 6 0.1830894451511682 1104 7 0.18134074772756748 1104 8 0.3473445614248618 1104 9 0.3485381852551299 1104 10-11 0.36823554746218434 1104 12-13 0.37832458128296054 1104 14-15 0.5576385025483361 1104 16-17 0.590961972296931 1104 18-19 0.5874334366053802 1104 20-21 0.7335550110718927 1104 22-23 0.4673091525576254 1104 24-25 0.5639704916921744 1104 26-27 0.5268781173091668 1104 28-29 0.6050159564055875 1104 30-31 0.7033269056962439 1104 32-33 0.5435895383206315 1104 34-35 0.6103514676287958 1104 36-37 0.6213280077983896 1104 38-39 0.690653443622729 1104 40-41 0.725078019204922 1104 42-43 0.6648082380560112 1104 44-45 0.5365212752543087 1104 46-47 0.5518369790167128 1104 48-49 0.747971614103129 1104 50-51 0.7996342383923931 1104 52-53 0.9645655116689298 1104 54-55 0.8436677026717803 1104 56-57 0.9442909588698498 1104 58-59 1.0196720297246245 1104 60-61 1.1306640545065747 1104 62-63 1.1458625978448609 1104 64-65 1.1227767893242309 1104 66-67 1.1643797822425448 1104 68-69 1.358498591373678 1104 70-71 1.2690981692472185 1104 72-73 1.5616633832994147 1104 74-75 1.691081928847769 1104 76-77 1.5593325038659138 1104 78-79 1.7131178906167612 1104 80-81 1.8605607141576463 1104 82-83 2.13464911534361 1104 84-85 2.291217885679327 1104 86-87 2.40700336293245 1104 88-89 2.5254731451058063 1104 90-91 2.751525997882883 1104 92-93 3.220404211360421 1104 94-95 3.3810486159126363 1104 96-97 3.3950775027929865 1104 98-99 3.791643505805137 1104 100-101 4.084120836784777 1105 1 0.22809420997933572 1105 2 0.25288141722159807 1105 3 0.14947565756412473 1105 4 0.19241051641719764 1105 5 0.2044261131139251 1105 6 0.15657770736748944 1105 7 0.2141171260036714 1105 8 0.178003436019452 1105 9 0.3544564214856152 1105 10-11 0.29778466997095165 1105 12-13 0.39319093193945065 1105 14-15 0.5407511291934242 1105 16-17 0.5949916539980009 1105 18-19 0.48324479310151247 1105 20-21 0.4110467925259016 1105 22-23 0.4052647050713176 1105 24-25 0.3757266001306121 1105 26-27 0.4716568844279152 1105 28-29 0.4648687180357456 1105 30-31 0.5880368776792224 1105 32-33 0.5510523534016869 1105 34-35 0.504293367089879 1105 36-37 0.6828386085636211 1105 38-39 0.711568842826594 1105 40-41 0.6039145733546007 1105 42-43 0.6555391091004594 1105 44-45 0.6976992705251988 1105 46-47 0.8135263014199481 1105 48-49 0.8068350592911102 1105 50-51 0.9587561069000756 1105 52-53 0.9385351852894459 1105 54-55 1.0119838556195262 1105 56-57 1.0138342086231305 1105 58-59 1.0408234430495966 1105 60-61 1.215637748536004 1105 62-63 1.3528794367569432 1105 64-65 1.475937259056355 1105 66-67 1.3827548835255037 1105 68-69 1.292669086738396 1105 70-71 1.4495502831080245 1105 72-73 1.3485748658610852 1105 74-75 1.5076923333393246 1105 76-77 1.3303149098852671 1105 78-79 1.6491567391151243 1105 80-81 1.9463516263996894 1105 82-83 2.235679607308313 1105 84-85 2.243805982531221 1105 86-87 2.467796979732949 1105 88-89 2.586164430245745 1105 90-91 2.7774383172018204 1105 92-93 2.8900315784010147 1105 94-95 3.1416067705427295 1105 96-97 3.6547979066442764 1105 98-99 3.9080505716572738 1105 100-101 4.453525767691588 1106 1 0.06637868178201245 1106 2 0.16199594426906927 1106 3 0.11618699268996124 1106 4 0.0965746254543447 1106 5 0.044741847074348584 1106 6 0.09064749390989135 1106 7 0.17930987760957606 1106 8 0.03891104147303537 1106 9 0.02581385273221315 1106 10-11 0.20545366804299903 1106 12-13 0.03805290640061543 1106 14-15 0.04283188688576445 1106 16-17 0.053488459482714745 1106 18-19 0.23291388627415444 1106 20-21 0.3125805056852329 1106 22-23 0.18558363786978305 1106 24-25 0.10425331907378421 1106 26-27 0.18638450769125114 1106 28-29 0.4590442391437506 1106 30-31 0.3524720666268095 1106 32-33 0.32025683696225826 1106 34-35 0.24554540959119464 1106 36-37 0.32919776891186103 1106 38-39 0.36729843726580214 1106 40-41 0.3752140796960788 1106 42-43 0.4224432390597386 1106 44-45 -0.011926854471560944 1106 46-47 -0.29973050475640406 1106 48-49 0.1827311662187583 1106 50-51 0.029463828203468267 1106 52-53 0.35814557546122927 1106 54-55 0.36704947502696683 1106 56-57 0.366688523164008 1106 58-59 0.6175575158620674 1106 60-61 0.4349783988511362 1106 62-63 0.6114203343336087 1106 64-65 0.36714477719031535 1106 66-67 0.30347319557647623 1106 68-69 0.07499982930109894 1106 70-71 0.4765028711409123 1106 72-73 0.8957972802057093 1106 74-75 1.1162425248373289 1106 76-77 1.1336516437851714 1106 78-79 1.4278425354096633 1106 80-81 1.317361508215594 1106 82-83 1.684689264341003 1106 84-85 1.4980409847358267 1106 86-87 1.8398190344244298 1106 88-89 2.275062733132483 1106 90-91 2.304451856067704 1106 92-93 1.8950941718805723 1106 94-95 2.373071233181168 1106 96-97 2.9222026115298547 1106 98-99 3.176883730639524 1106 100-101 3.441530557303146 1107 1 0.06525577920508852 1107 2 0.11336759894652104 1107 3 0.10572864314566743 1107 4 0.11524096652203752 1107 5 -0.024426137164454076 1107 6 0.01523587333755927 1107 7 0.06158473833323086 1107 8 0.07910540750699369 1107 9 0.12517727913718346 1107 10-11 0.08342524159663611 1107 12-13 0.05871464493360179 1107 14-15 0.10984946923057493 1107 16-17 0.2073480490624391 1107 18-19 0.2439336871069102 1107 20-21 0.08992419748096125 1107 22-23 0.06017408678247449 1107 24-25 0.08120753378699419 1107 26-27 0.16135325846730098 1107 28-29 0.20394958089850945 1107 30-31 0.1254882582776773 1107 32-33 0.21182678969522328 1107 34-35 0.17615060979317576 1107 36-37 0.21830793173894136 1107 38-39 0.0597835162787419 1107 40-41 0.1118505870675861 1107 42-43 0.20099799349464575 1107 44-45 0.16596545780544858 1107 46-47 0.2050328289356429 1107 48-49 0.24146956675517117 1107 50-51 0.34058648244219114 1107 52-53 0.4686753222390436 1107 54-55 0.5315973339377535 1107 56-57 0.37530875610524816 1107 58-59 0.638571894644052 1107 60-61 0.6066647394221505 1107 62-63 0.6961711269463748 1107 64-65 0.7364116828135145 1107 66-67 0.9005628787797058 1107 68-69 0.9170948103860077 1107 70-71 0.9674635674812357 1107 72-73 1.183497709830803 1107 74-75 1.246549098349579 1107 76-77 1.0891622685213136 1107 78-79 1.296084632046334 1107 80-81 1.5068381768413701 1107 82-83 1.6846983700751323 1107 84-85 1.8554687804031538 1107 86-87 2.035310247390999 1107 88-89 2.2602270088541125 1107 90-91 2.3223762867523696 1107 92-93 2.4877435680048414 1107 94-95 2.6682381578436356 1107 96-97 2.9873201996692202 1107 98-99 3.1724622758743273 1107 100-101 3.279181940133043 1108 1 0.13592559238601964 1108 2 0.16607771171400643 1108 3 0.16887514697813089 1108 4 0.2078911275513704 1108 5 0.11311310297738686 1108 6 0.06999015102161366 1108 7 0.11818326356116415 1108 8 0.16794657530613222 1108 9 0.1479052631876101 1108 10-11 0.13957737597867492 1108 12-13 0.28042848844877 1108 14-15 0.3973768160492668 1108 16-17 0.25627733699666777 1108 18-19 0.3181554568959868 1108 20-21 0.23085929436459907 1108 22-23 0.29562334923994626 1108 24-25 0.287652150023888 1108 26-27 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60-61 0.3671565793768039 2316 62-63 0.20940687391705382 2316 64-65 0.20757296834058891 2316 66-67 0.44079619921202706 2316 68-69 0.2937822102185237 2316 70-71 0.39613068135058427 2316 72-73 0.5108717683892117 2316 74-75 0.583795638234772 2316 76-77 0.6399068353565696 2316 78-79 0.6767298488511493 2316 80-81 0.9321550237255956 2316 82-83 1.2149379188788707 2316 84-85 1.4809782454406033 2316 86-87 1.6524865652261447 2316 88-89 1.8281917777288044 2316 90-91 1.8945527126045434 2316 92-93 1.8323194290927525 2316 94-95 2.495014918878123 2316 96-97 2.7881505393443113 2316 98-99 3.0146618539854657 2316 100-101 3.315980685378001 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 11.0 4 33.0 5 88.0 6 172.0 7 464.0 8 980.0 9 1922.0 10 3072.0 11 4787.0 12 7022.0 13 9455.0 14 12304.0 15 16263.0 16 20427.0 17 25650.0 18 24120.0 19 20800.0 20 20546.0 21 22166.0 22 24112.0 23 27157.0 24 31690.0 25 37206.0 26 43181.0 27 52045.0 28 62183.0 29 74034.0 30 87004.0 31 102986.0 32 123045.0 33 148738.0 34 184466.0 35 233184.0 36 288852.0 37 305233.0 38 152035.0 39 8237.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74842772788006 19.05157622491983 14.903572365884028 23.29642368131608 2 16.221591235673475 20.610835852808975 38.42435250433963 24.74322040717792 3 19.418349022760356 23.892513061499702 30.100166891195308 26.588971024544634 4 13.554604228390835 16.818052704587178 35.69390450959105 33.93343855743094 5 13.855238761266339 37.5560728959149 33.77900262708706 14.809685715731696 6 33.0789447316096 35.77516668397931 16.565395367136805 14.58049321727428 7 29.24362749198639 32.79746873842313 19.796532017204783 18.1623717523857 8 27.520483307701433 34.146751765200115 19.198510438604313 19.13425448849414 9 26.917359861817538 16.16574909660179 19.49253426754192 37.424356774038756 10-11 25.537787370718224 26.26163202759237 26.673619301421077 21.52696130026833 12-13 26.56887015242173 23.490766539472364 27.067773021141956 22.87259028696395 14-15 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28.471313072993855 23.053751558322638 40-41 22.899115774009736 25.902928428326398 28.58199091389018 22.615964883773685 42-43 22.73085280831607 25.918923857098044 28.19963710284423 23.150586231741666 44-45 22.602386180250054 26.069665027433675 28.627268385754622 22.700680406561652 46-47 23.20149820377336 26.47848572762806 27.67420824462511 22.645807823973467 48-49 22.73164545791327 26.602399991910552 27.46188077809451 23.204073772081664 50-51 23.074412802653338 26.752077746941865 27.495226766510054 22.67828268389474 52-53 23.565157279996914 26.26011525623784 27.466454073542266 22.70827339022298 54-55 22.71941936154038 26.658290718186638 27.704242455441396 22.918047464831588 56-57 22.851033748622264 26.767337386023826 27.375493755038672 23.006135110315242 58-59 22.764118153730752 26.222494730368613 27.45937580722112 23.554011308679517 60-61 22.211944436528636 26.68205346940764 27.864652516875232 23.241349577188494 62-63 22.56231861942552 26.629885727365405 27.947937053069534 22.859858600139543 64-65 22.168923285381908 26.75547899179748 27.566606945709697 23.50899077711091 66-67 22.01760330895694 26.75804998964687 27.64129494792525 23.58305175347094 68-69 22.881294388665918 26.240584500944074 27.687450474980558 23.190670635409447 70-71 22.593481376345906 26.38030789539242 27.265114166920227 23.761096561341454 72-73 22.41675968384043 26.585009193704845 27.330479504392546 23.667751618062177 74-75 22.364862566726448 27.041735112889153 27.064440720438814 23.528961599945582 76-77 22.44536635543744 26.511830356937665 27.515450384570485 23.52735290305441 78-79 22.400690544631228 26.776598883840137 27.40596707043427 23.416743501094373 80-81 22.67359234296346 26.67067922002949 27.15402413599109 23.501704301015963 82-83 22.49099465769587 26.7271021619716 27.30311313430956 23.478790046022965 84-85 22.21513343568152 26.932013532023646 27.240804750916997 23.612048281377838 86-87 22.57651290697108 26.934069237366742 26.803326460278925 23.686091395383258 88-89 22.303384885221668 27.030361194150586 26.55915046017966 24.10710346044808 90-91 22.056369231334934 26.896865541641485 26.93788767060954 24.108877556414047 92-93 22.23372826960002 26.506945569578072 27.131443568468068 24.127882592353842 94-95 21.820594445675226 26.162904001242833 27.501569628538096 24.51493192454384 96-97 22.109447463176927 25.79609812867185 27.455859655444705 24.638594752706517 98-99 21.722050270398967 26.256484197540626 26.988625134096377 25.032840397964033 100-101 21.789293170158178 25.81124882679171 27.43094273088877 24.968515272161344 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 443.0 1 313.0 2 244.0 3 316.5 4 293.5 5 296.0 6 548.0 7 1186.0 8 1824.0 9 2094.5 10 1877.0 11 1547.0 12 1527.5 13 1651.0 14 1797.5 15 1987.5 16 2169.5 17 2375.5 18 2694.0 19 3027.5 20 3372.5 21 3930.5 22 4703.0 23 5698.5 24 6994.5 25 8656.5 26 11112.5 27 14217.0 28 16980.5 29 19684.5 30 23044.5 31 28570.5 32 36947.0 33 39291.5 34 37571.5 35 39682.0 36 44006.5 37 48310.0 38 51681.0 39 54890.0 40 57899.5 41 61485.0 42 63870.0 43 66681.5 44 70000.0 45 74535.0 46 76981.5 47 78768.5 48 81330.0 49 80287.0 50 78808.5 51 77387.5 52 76358.0 53 78348.0 54 81141.0 55 78418.5 56 72717.0 57 66793.5 58 62461.5 59 56148.0 60 45047.0 61 37140.0 62 32442.5 63 24753.0 64 17003.0 65 12480.5 66 9282.0 67 7281.5 68 5697.0 69 4433.5 70 3466.0 71 2402.5 72 1709.5 73 1411.5 74 1093.0 75 754.5 76 519.0 77 354.0 78 245.5 79 154.5 80 95.0 81 57.5 82 36.5 83 22.5 84 15.0 85 8.5 86 6.0 87 4.5 88 3.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.596276831915075E-5 2 0.04361866713487407 3 2.298138415957538E-4 4 0.0 5 0.09946343064264224 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011720505921383442 26-27 0.01178945007386217 28-29 0.0 30-31 0.0 32-33 5.745346039893845E-4 34-35 0.015328583234436777 36-37 0.0031024868615426755 38-39 0.011858394226340895 40-41 0.007147210473627942 42-43 0.005814290192372571 44-45 0.0028956544041064975 46-47 9.19255366383015E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.6086968911702764E-4 68-69 2.7577660991490453E-4 70-71 0.0 72-73 2.2981384159575376E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 9.19255366383015E-5 82-83 9.652181347021659E-4 84-85 0.0043205002220001705 86-87 0.001286957512936221 88-89 9.19255366383015E-5 90-91 8.732925980638643E-4 92-93 2.9875799407447993E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 7.35404293106412E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2175674.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.6542145217813 #Duplication Level Percentage of deduplicated Percentage of total 1 71.36199921913288 33.29338020272615 2 14.81456562489671 13.823238454218764 3 5.590663325229333 7.824840182829136 4 2.690909223370465 5.021690246630623 5 1.5112379401699836 3.5252809527072646 6 0.9031933109313556 2.5282664689697625 7 0.5958703980470078 1.9459905764365022 8 0.4232482896588791 1.5797053201377902 9 0.31162064283966234 1.30845746884113 >10 1.6122600982866702 13.652850376339638 >50 0.11184273715535323 3.601721362826097 >100 0.06341994355566019 5.719585290471549 >500 0.004338138235984799 1.418310964726561 >1k 0.004633920388438298 4.158152513113818 >5k 1.9718810163567226E-4 0.5985296190251665 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6984 0.3210039739409489 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6028 0.2770635674278407 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4249 0.19529580258807155 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 4113 0.18904486609666707 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 4000 0.18385107327660302 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 3487 0.16027217312887868 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3463 0.15916906668921904 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3323 0.15273427912453796 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 3183 0.14629949155985686 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2756 0.1266733894875795 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2633 0.12101996898432393 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2432 0.11178145255217463 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2417 0.11109201102738739 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2353 0.10815039385496172 No Hit TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA 2289 0.10520877668253607 No Hit ATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACTTCATTT 2176 0.10001498386247205 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 4.596276831915076E-5 0.0 0.0 0.0 0.0 3 4.596276831915076E-5 0.0 0.0 0.0 0.0 4 4.596276831915076E-5 0.0 0.0 0.0 0.0 5 4.596276831915076E-5 0.0 0.0 0.0 0.0 6 4.596276831915076E-5 0.0 0.0 0.0 1.3788830495745227E-4 7 4.596276831915076E-5 0.0 0.0 0.0 1.3788830495745227E-4 8 4.596276831915076E-5 0.0 0.0 0.0 1.3788830495745227E-4 9 4.596276831915076E-5 0.0 0.0 1.3788830495745227E-4 1.3788830495745227E-4 10-11 9.192553663830152E-5 0.0 0.0 1.83851073276603E-4 1.3788830495745227E-4 12-13 9.192553663830152E-5 0.0 0.0 2.2981384159575377E-4 2.298138415957538E-4 14-15 9.192553663830152E-5 0.0 0.0 2.7577660991490453E-4 2.9875799407447993E-4 16-17 1.3788830495745227E-4 0.0 0.0 3.2173937823405527E-4 3.4472076239363067E-4 18-19 2.5279522575532914E-4 0.0 0.0 3.4472076239363067E-4 5.515532198298091E-4 20-21 2.7577660991490453E-4 0.0 0.0 4.136649148723568E-4 5.515532198298091E-4 22-23 2.7577660991490453E-4 0.0 0.0 7.124229089468367E-4 5.975159881489599E-4 24-25 3.67702146553206E-4 0.0 0.0 0.0012409947446170703 6.434787564681105E-4 26-27 4.366462990319322E-4 0.0 0.0 0.0022062128793192363 7.583856772659875E-4 28-29 6.894415247872613E-4 0.0 0.0 0.005630439119095968 7.813670614255628E-4 30-31 7.354042931064121E-4 0.0 0.0 0.016592559363213424 8.273298297447136E-4 32-33 7.354042931064121E-4 0.0 0.0 0.03968885044358668 8.732925980638644E-4 34-35 8.273298297447136E-4 0.0 0.0 0.07395409422551356 0.0010111809030213166 36-37 9.192553663830151E-4 0.0 0.0 0.13370569304040955 0.0010111809030213166 38-39 0.0010571436713404673 0.0 0.0 0.22376973756178545 0.0010111809030213166 40-41 0.0010571436713404673 0.0 0.0 0.31888968659826794 0.0010111809030213166 42-43 0.0012409947446170703 0.0 0.0 0.4168133645022186 0.0010111809030213166 44-45 0.0013329202812553719 0.0 0.0 0.5391662537677979 0.0010571436713404673 46-47 0.0014248458178936735 0.0 0.0 0.6884992880367187 0.0011031064396596181 48-49 0.0014248458178936735 0.0 0.0 0.8395329447334481 0.0011031064396596181 50-51 0.0014248458178936735 0.0 0.0 1.0084920810746463 0.0011950319762979197 52-53 0.0014708085862128243 0.0 0.0 1.1737282331819934 0.0014248458178936735 54-55 0.0014708085862128243 0.0 0.0 1.353258806236596 0.0014248458178936735 56-57 0.0014937899703723995 0.0 0.0 1.5630558622293598 0.0014248458178936735 58-59 0.0015627341228511256 0.0 0.0 1.7833324293988897 0.0014248458178936735 60-61 0.0016086968911702764 0.0 0.0 2.0266823062646333 0.0014248458178936735 62-63 0.0016546596594894272 0.0 0.0 2.262356400821079 0.0015167713545319748 64-65 0.001700622427808578 0.0 0.0 2.5121180838673443 0.0015397527386915503 66-67 0.001769566580287304 0.0 0.0 2.805958061731675 0.0016546596594894272 68-69 0.0017925479644468794 0.0 0.0 3.133396823237305 0.001700622427808578 70-71 0.0017925479644468794 0.0 0.0 3.467867888295765 0.001700622427808578 72-73 0.0017925479644468794 0.0 0.0 3.8165874115331615 0.001884473501085181 74-75 0.0017925479644468794 0.0 0.0 4.17498209750174 0.002045343190202209 76-77 0.0017925479644468794 0.0 0.0 4.563735191945117 0.002068324574361784 78-79 0.0017925479644468794 0.0 0.0 5.004701991199049 0.0021142873426809347 80-81 0.0019304362694043317 0.0 0.0 5.459595509253685 0.0021602501110000857 82-83 0.0019763990377234825 0.0 0.0 5.926554253992096 0.002252175647638387 84-85 0.001999380421883058 0.0 0.0 6.401625427338838 0.002321119800117113 86-87 0.0020913059585213594 0.0 0.0 6.901861216340316 0.00243602672091499 88-89 0.0021142873426809347 0.0 0.0 7.432294544127475 0.0025279522575532916 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4790 0.0 50.55408 1 GTATCAA 8370 0.0 40.469563 1 ATCAACG 11005 0.0 30.761963 3 TCAACGC 11060 0.0 30.608986 4 TAAGGTG 1620 0.0 30.524069 5 TATCAAC 11130 0.0 30.43398 2 CAACGCA 11270 0.0 29.996445 5 AACGCAG 11610 0.0 29.044968 6 AGGTAAG 1480 0.0 28.930422 2 GTAAGGT 1755 0.0 27.09237 4 ACGCAGA 13260 0.0 25.394957 7 GGTAAGG 1785 0.0 25.305183 3 CGCAGAG 13445 0.0 25.0102 8 TACATGG 9750 0.0 23.567661 2 GTACATG 10070 0.0 23.054956 1 TAAGGTA 825 0.0 23.053143 4 AAGGTAA 1715 0.0 23.02438 1 AAGGTGT 2265 0.0 22.646458 6 GCAGAGT 14910 0.0 22.266106 9 GAGTACT 7890 0.0 22.122036 12-13 >>END_MODULE