FastQCFastQC Report
Thu 26 May 2016
SRR938460_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938460_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185952
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70630.3231086501441935No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55130.2522013292149141No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA38700.17703956902987805No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA37500.1715499699901919No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA37190.17013182357160633No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37030.1693998770329815No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA33070.15128420020201724No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC29190.1335344966403654No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG28770.13161313697647523No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA27740.12690123113407797No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA26870.12292127183030552No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG25370.11605927303069782No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23960.10960899415906664No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT22680.10375342185006808No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48450.045.971491
GTATCAA84100.040.265011
TATCAAC108800.031.1882442
ATCAACG109950.030.6459163
TCAACGC111550.030.2489554
CAACGCA113200.029.7653835
TAAGGTG16450.029.7565775
AACGCAG116800.028.9233726
GTAAGGT16900.026.9964184
AGGTAAG15200.026.8891142
TAAGGTA7550.025.8082474
ACGCAGA135100.024.824597
CGCAGAG136450.024.6137968
GTGTAAG8550.024.5443781
GTACATG101400.023.8941291
TACATGG98700.023.8346982
GGTAAGG17750.023.8294093
AAGGTAA16450.023.774611
GCAGAGT147550.022.4391529
ACATGGG102050.022.2605823
Produced by FastQC (version 0.11.5)