##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938460_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2185952 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94558983911815 31.0 31.0 34.0 28.0 34.0 2 31.122590066021576 31.0 31.0 34.0 27.0 34.0 3 31.16539384213377 31.0 31.0 34.0 27.0 34.0 4 34.8455922179444 37.0 35.0 37.0 32.0 37.0 5 34.83787292676143 37.0 35.0 37.0 32.0 37.0 6 34.806287603753425 37.0 35.0 37.0 32.0 37.0 7 34.7208319304358 37.0 35.0 37.0 31.0 37.0 8 34.66861211957079 37.0 35.0 37.0 30.0 37.0 9 36.13507798890369 39.0 35.0 39.0 31.0 39.0 10-11 35.9585148713238 38.0 35.0 39.0 30.0 39.0 12-13 35.77505635988347 38.0 35.0 39.0 30.0 39.0 14-15 36.88079495798627 39.0 36.0 41.0 31.0 41.0 16-17 36.81010058775307 39.0 36.0 41.0 30.0 41.0 18-19 36.81351717695539 39.0 36.0 41.0 30.0 41.0 20-21 36.7391930380905 39.0 36.0 41.0 30.0 41.0 22-23 36.590192511088986 39.0 36.0 41.0 30.0 41.0 24-25 36.4401642854006 39.0 36.0 41.0 29.5 41.0 26-27 36.20796179422055 39.0 35.0 40.5 29.0 41.0 28-29 35.979882678119196 39.0 35.0 40.0 27.5 41.0 30-31 35.681498495849866 38.0 34.5 40.0 27.0 41.0 32-33 35.36685389249169 38.0 34.0 40.0 26.5 41.0 34-35 35.048424439328954 38.0 34.0 40.0 25.5 41.0 36-37 34.79958754812549 38.0 33.5 40.0 25.0 41.0 38-39 34.54112007034006 38.0 33.0 40.0 24.0 41.0 40-41 34.257348285781205 38.0 33.0 40.0 24.0 41.0 42-43 33.817805697471854 38.0 33.0 40.0 22.5 41.0 44-45 33.32474500812461 37.0 32.0 40.0 19.5 41.0 46-47 32.812442816676665 37.0 31.0 40.0 16.5 41.0 48-49 32.296702992563425 37.0 30.5 39.5 13.0 41.0 50-51 31.551447607266766 35.5 30.0 38.5 9.0 40.0 52-53 31.566759242654918 36.0 30.0 39.0 8.5 40.0 54-55 31.562506633265507 36.0 30.0 39.0 7.5 40.0 56-57 31.14699819575178 35.5 29.5 39.0 3.5 40.0 58-59 30.698276540381492 35.0 29.0 39.0 2.0 40.0 60-61 30.14595791673376 35.0 28.0 38.5 2.0 40.0 62-63 29.440466213347776 34.0 27.0 38.0 2.0 40.0 64-65 28.78376103409407 34.0 26.0 37.5 2.0 40.0 66-67 28.103111824962305 34.0 25.5 37.0 2.0 39.0 68-69 27.275102335275434 33.0 23.5 36.0 2.0 39.0 70-71 26.447286125221414 32.0 21.0 36.0 2.0 39.0 72-73 25.588249421762235 31.5 18.5 35.0 2.0 38.0 74-75 24.691760386321384 31.0 12.0 35.0 2.0 37.0 76-77 23.777033530470934 30.5 7.0 34.0 2.0 36.5 78-79 22.82250273565019 29.5 2.0 34.0 2.0 36.0 80-81 21.90325107779128 29.0 2.0 34.0 2.0 35.5 82-83 20.929567758120946 27.5 2.0 33.5 2.0 35.0 84-85 19.979882220652602 26.0 2.0 33.0 2.0 35.0 86-87 19.031713413652266 25.5 2.0 32.5 2.0 34.5 88-89 17.94652673068759 23.0 2.0 32.0 2.0 34.0 90-91 16.804698822298022 19.0 2.0 31.5 2.0 34.0 92-93 15.665933195239418 8.0 2.0 31.0 2.0 34.0 94-95 14.42220917934154 2.0 2.0 30.5 2.0 34.0 96-97 13.008330009076136 2.0 2.0 29.5 2.0 34.0 98-99 11.3212387554713 2.0 2.0 28.0 2.0 34.0 100-101 8.08690995959655 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7254595159410862 1101 2 0.5219932992954952 1101 3 0.6156796233914008 1101 4 0.5250939625392661 1101 5 0.49989012009478984 1101 6 0.5967131529181131 1101 7 0.6038504930826747 1101 8 0.5773536620063666 1101 9 0.6262694901537245 1101 10-11 0.7379562550651215 1101 12-13 0.6619983108793477 1101 14-15 0.7574001807863766 1101 16-17 0.8146267881431157 1101 18-19 0.9435312871404591 1101 20-21 0.969607602773749 1101 22-23 0.8955764389675309 1101 24-25 1.0091434963727437 1101 26-27 1.0288928657267462 1101 28-29 1.0771263504201514 1101 30-31 1.021758452642878 1101 32-33 1.1311410032792466 1101 34-35 1.0863722310858677 1101 36-37 1.1250961850367318 1101 38-39 1.3296899954297814 1101 40-41 1.1474266712795753 1101 42-43 1.3961189390725721 1101 44-45 1.380176992806689 1101 46-47 1.3688683755290825 1101 48-49 1.3005772733997887 1101 50-51 1.2965368330814186 1101 52-53 1.3472463379415913 1101 54-55 1.6629734422309816 1101 56-57 1.9146542191036353 1101 58-59 1.699967369162085 1101 60-61 1.8677954996542567 1101 62-63 1.6467176827181937 1101 64-65 1.6347629768358445 1101 66-67 1.964535003957021 1101 68-69 2.0410461991659474 1101 70-71 2.248592128843704 1101 72-73 2.4580115312249973 1101 74-75 2.338995124083013 1101 76-77 2.7885639503501274 1101 78-79 2.866917780496337 1101 80-81 3.229400740160063 1101 82-83 3.234240983869313 1101 84-85 3.2441027056966085 1101 86-87 3.423135941333758 1101 88-89 3.6828506890440202 1101 90-91 3.734372770147548 1101 92-93 3.5022811574029618 1101 94-95 3.4206237247840026 1101 96-97 3.413393574500242 1101 98-99 3.158956619690338 1101 100-101 2.5254854473762425 1102 1 0.7217232522048249 1102 2 0.6201810764306401 1102 3 0.5921901304282251 1102 4 0.47819594827282685 1102 5 0.5202371414944409 1102 6 0.6410509204137753 1102 7 0.65128831841524 1102 8 0.6744232590759651 1102 9 0.8168497870498115 1102 10-11 0.9142007123622164 1102 12-13 0.8748400305631776 1102 14-15 0.904911266192201 1102 16-17 1.0159898110850847 1102 18-19 1.0002596465003961 1102 20-21 0.8941208088658996 1102 22-23 0.7310188912520843 1102 24-25 0.9448828447436668 1102 26-27 0.9104024088152372 1102 28-29 0.8133920145805504 1102 30-31 0.642747463631899 1102 32-33 0.7777922467725986 1102 34-35 0.7027998385661007 1102 36-37 0.8754181437797399 1102 38-39 1.148186236031279 1102 40-41 1.0562410148307606 1102 42-43 1.2644262458009337 1102 44-45 1.2198049087503904 1102 46-47 1.2762580034450224 1102 48-49 1.2652639901917766 1102 50-51 1.383109032811518 1102 52-53 1.416608204145561 1102 54-55 1.4268812887704065 1102 56-57 1.870339586367951 1102 58-59 1.9022750614697763 1102 60-61 1.8529410558524475 1102 62-63 1.8014217505801575 1102 64-65 2.137477455339795 1102 66-67 2.3181883681367026 1102 68-69 2.1746436543423506 1102 70-71 2.0087733511301877 1102 72-73 2.153394218712947 1102 74-75 2.2723940059029495 1102 76-77 2.509034357136322 1102 78-79 2.83622759349036 1102 80-81 3.205672188010457 1102 82-83 3.3512450324523115 1102 84-85 3.213318051754058 1102 86-87 3.2077185517058417 1102 88-89 3.4257849477677524 1102 90-91 3.188623782293295 1102 92-93 3.330023590408551 1102 94-95 3.230425151427534 1102 96-97 3.016055999794249 1102 98-99 2.63306689537956 1102 100-101 2.1028885705688403 1103 1 0.5998507744001422 1103 2 0.5539731838177531 1103 3 0.591637902400528 1103 4 0.6500917895709861 1103 5 0.3662674715568883 1103 6 0.4984316603993335 1103 7 0.4688321780643605 1103 8 0.446336092005744 1103 9 0.6042504922025245 1103 10-11 0.5489316073964048 1103 12-13 0.6108410500610759 1103 14-15 0.6988070225322076 1103 16-17 0.753074047098842 1103 18-19 0.7769173925604633 1103 20-21 0.6378232853962089 1103 22-23 0.8300546161575681 1103 24-25 0.7509191218771818 1103 26-27 0.8601581541106782 1103 28-29 0.7437688040117507 1103 30-31 0.7935949212590074 1103 32-33 0.9620579958911506 1103 34-35 0.8761909434469501 1103 36-37 0.9829666761275533 1103 38-39 0.864298550716299 1103 40-41 1.0840393241634132 1103 42-43 1.1706027038953124 1103 44-45 1.0689489553074552 1103 46-47 1.3125903597595396 1103 48-49 1.1686240853449092 1103 50-51 1.4658327913604268 1103 52-53 1.418953049309323 1103 54-55 1.6121750316020602 1103 56-57 1.5704585277893024 1103 58-59 1.7955761107030277 1103 60-61 1.8474782462861583 1103 62-63 1.905695145932949 1103 64-65 1.9121131972369092 1103 66-67 2.1670308132324934 1103 68-69 2.190904645804057 1103 70-71 2.483884858712706 1103 72-73 2.6679730386441314 1103 74-75 2.703025669808472 1103 76-77 2.6156441018370558 1103 78-79 2.89160332094459 1103 80-81 3.1362188937578743 1103 82-83 3.2403873798462186 1103 84-85 3.3305135829549393 1103 86-87 3.3146383936836656 1103 88-89 3.749815362788354 1103 90-91 3.634577650013444 1103 92-93 3.861305184223596 1103 94-95 3.654965315396778 1103 96-97 3.395780735361983 1103 98-99 3.3517491943473186 1103 100-101 2.543383876630605 1104 1 0.6889735506528929 1104 2 0.5551766433423495 1104 3 0.5555253486967366 1104 4 0.4714206534899077 1104 5 0.5651725999176591 1104 6 0.6405343085498529 1104 7 0.5155625136526325 1104 8 0.4292713821692118 1104 9 0.7979229434784898 1104 10-11 0.6906589191605477 1104 12-13 0.8001477287780787 1104 14-15 0.8120691330040728 1104 16-17 0.9476322877502881 1104 18-19 0.8743969395938578 1104 20-21 0.7332709125373356 1104 22-23 0.8088269210759407 1104 24-25 1.0132567739957707 1104 26-27 0.9785550327148158 1104 28-29 1.055733592759978 1104 30-31 1.0518534458576525 1104 32-33 1.0935429745670078 1104 34-35 1.1811747451920027 1104 36-37 1.3366924995856877 1104 38-39 1.2951867456619084 1104 40-41 1.1433491053914224 1104 42-43 1.3149233101889166 1104 44-45 1.342879656902106 1104 46-47 1.4981619974132272 1104 48-49 1.5804101424805665 1104 50-51 1.4684924076832289 1104 52-53 1.5678941488122433 1104 54-55 1.9539897982055514 1104 56-57 2.12171942873152 1104 58-59 2.048189638999954 1104 60-61 2.057592657000164 1104 62-63 2.145407062048246 1104 64-65 2.2016074879421943 1104 66-67 2.304742846254001 1104 68-69 2.585779432506424 1104 70-71 2.66326822340838 1104 72-73 2.9082008034198417 1104 74-75 2.77540466625857 1104 76-77 2.7582332596851735 1104 78-79 3.053642300173504 1104 80-81 3.284197540388618 1104 82-83 3.2229525044749856 1104 84-85 3.4157911565215464 1104 86-87 3.4848343914444655 1104 88-89 3.3247869793089997 1104 90-91 3.3810515788040725 1104 92-93 3.5188871141203393 1104 94-95 3.6501551868224293 1104 96-97 3.5084317860565637 1104 98-99 3.15446265497353 1104 100-101 2.1398929896946495 1105 1 0.6089919198161411 1105 2 0.46141897731450143 1105 3 0.4445455455666121 1105 4 0.3548555865479557 1105 5 0.4080429951728206 1105 6 0.4824623841497626 1105 7 0.4431462319095232 1105 8 0.4130037047799462 1105 9 0.5274583643994077 1105 10-11 0.5869780117875152 1105 12-13 0.717979213055024 1105 14-15 0.7031047887723219 1105 16-17 0.8505325475475161 1105 18-19 0.7609933111399059 1105 20-21 0.7028981625295927 1105 22-23 0.5785356769042167 1105 24-25 0.7732492973946918 1105 26-27 0.6390110132840263 1105 28-29 0.8148660558604846 1105 30-31 0.8613485199245687 1105 32-33 0.8211675922598474 1105 34-35 0.9006211501860051 1105 36-37 0.837375901112658 1105 38-39 1.085878754071203 1105 40-41 1.0831312792655154 1105 42-43 1.3159284232463477 1105 44-45 1.3758083076895744 1105 46-47 1.4143642022151681 1105 48-49 1.333528881455095 1105 50-51 0.9874815511226203 1105 52-53 1.1015997632959156 1105 54-55 1.5734730838577669 1105 56-57 1.688887647232466 1105 58-59 1.5910787885512327 1105 60-61 1.9132360675024032 1105 62-63 1.8663056691231326 1105 64-65 1.8558362468180398 1105 66-67 1.9976209441664956 1105 68-69 2.1183001354911184 1105 70-71 2.2284337510027328 1105 72-73 2.2622727043770645 1105 74-75 2.136500846494055 1105 76-77 2.3035194427086836 1105 78-79 2.5411757167290254 1105 80-81 2.911199080082838 1105 82-83 3.2869149104837305 1105 84-85 3.125719546925712 1105 86-87 3.281962095642328 1105 88-89 3.415359763978703 1105 90-91 3.172383451981492 1105 92-93 3.153075358693105 1105 94-95 3.4107492710141454 1105 96-97 3.4351648630253653 1105 98-99 3.034568281848438 1105 100-101 2.4683554388215274 1106 1 0.44199119224992245 1106 2 0.29426670833770885 1106 3 0.26443899772180757 1106 4 0.31264628023085805 1106 5 0.3813285887756024 1106 6 -0.08741369516416597 1106 7 0.39253558700453794 1106 8 0.019800630080077042 1106 9 0.4060937884181399 1106 10-11 0.41744629149109613 1106 12-13 0.5457373295208825 1106 14-15 0.5618370067273801 1106 16-17 0.04558957651537554 1106 18-19 0.5194758625279405 1106 20-21 0.6882384148586027 1106 22-23 0.49684920519850806 1106 24-25 0.5724489745528771 1106 26-27 0.5349617179515818 1106 28-29 0.7237930170868125 1106 30-31 0.5764588311872743 1106 32-33 0.6213981277703553 1106 34-35 0.5936631388781706 1106 36-37 0.5936562878865246 1106 38-39 0.5737382776953339 1106 40-41 0.855512522290212 1106 42-43 0.16543398799764475 1106 44-45 0.7289056550450894 1106 46-47 0.6957014588802721 1106 48-49 0.6247462613292285 1106 50-51 0.5808415256033825 1106 52-53 0.7433816139933 1106 54-55 0.6259953692361968 1106 56-57 1.205052762064856 1106 58-59 1.2626743186656917 1106 60-61 1.6655313756631145 1106 62-63 1.405388491946109 1106 64-65 1.4539287507091352 1106 66-67 1.5152813792159314 1106 68-69 1.7440895532120848 1106 70-71 1.5112255121734677 1106 72-73 1.6579686771431454 1106 74-75 2.01704812029757 1106 76-77 2.006840502025007 1106 78-79 2.4122773976665393 1106 80-81 2.1015141606300283 1106 82-83 2.2689135072605 1106 84-85 2.346153273513192 1106 86-87 2.1322345771454856 1106 88-89 2.0016672021502266 1106 90-91 2.2598816623695335 1106 92-93 2.4738546164415993 1106 94-95 2.1097652146775783 1106 96-97 2.0218951101048432 1106 98-99 1.723404159151805 1106 100-101 1.260366527299107 1107 1 0.14469463110840763 1107 2 0.1526321519682483 1107 3 0.12818722878036226 1107 4 0.23609787388215864 1107 5 0.25609177155067897 1107 6 0.16670011510846905 1107 7 0.16464145353465653 1107 8 0.3038638749572584 1107 9 0.32588704773738897 1107 10-11 0.2497076674945049 1107 12-13 0.31138118788934577 1107 14-15 0.2697513324595633 1107 16-17 0.3034186177824054 1107 18-19 0.3607629255585465 1107 20-21 0.46354502128743746 1107 22-23 0.4396529274507941 1107 24-25 0.31720086273519854 1107 26-27 0.36721098828216014 1107 28-29 0.3481954508112821 1107 30-31 0.5033360276102883 1107 32-33 0.6732270528229236 1107 34-35 0.6814743605947768 1107 36-37 0.6403330385786674 1107 38-39 0.7933666577505107 1107 40-41 0.7044279750605398 1107 42-43 0.850238886921332 1107 44-45 0.6077085008466625 1107 46-47 0.5566084885234304 1107 48-49 0.6083521538865355 1107 50-51 0.9445377022687325 1107 52-53 0.866603139332284 1107 54-55 1.0140251588759135 1107 56-57 1.004015366430881 1107 58-59 1.0857605025823354 1107 60-61 1.246695977707276 1107 62-63 1.3369175957994628 1107 64-65 1.2386917034764338 1107 66-67 1.4499761165167797 1107 68-69 1.4268827919516944 1107 70-71 1.5975186825160215 1107 72-73 1.5746347385261679 1107 74-75 1.8189690484637175 1107 76-77 1.7671993262736372 1107 78-79 2.0984823176540957 1107 80-81 2.2971799665833537 1107 82-83 2.3466703624003884 1107 84-85 2.313881062932591 1107 86-87 2.5576817909643523 1107 88-89 2.733698146671138 1107 90-91 2.6880450865317265 1107 92-93 2.356692283000527 1107 94-95 2.494904472285091 1107 96-97 2.2365704541178015 1107 98-99 1.8427158548055047 1107 100-101 1.4053507233432132 1108 1 0.27918494098400615 1108 2 0.18667181732174498 1108 3 0.01746065014003051 1108 4 0.06658701232605324 1108 5 0.15989676571698652 1108 6 0.20641465372112577 1108 7 0.3037596695793283 1108 8 0.3055864803039796 1108 9 0.19978336289005938 1108 10-11 0.2015205237697799 1108 12-13 0.20026103447684562 1108 14-15 0.2050736592157989 1108 16-17 0.30034811505234416 1108 18-19 0.2171398599930967 1108 20-21 0.3507921849302562 1108 22-23 0.29104855504439087 1108 24-25 0.297019666279148 1108 26-27 0.23945885121714383 1108 28-29 0.189559755747716 1108 30-31 0.16767804061431235 1108 32-33 0.14859019195953493 1108 34-35 0.5946964266886781 1108 36-37 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-1.604930523963425 2316 46-47 -1.0365457050928235 2316 48-49 -1.0636199556140653 2316 50-51 -1.0160266425627427 2316 52-53 -1.0302509976486576 2316 54-55 -1.1874108596936637 2316 56-57 -1.1386734719502165 2316 58-59 -0.9786132840884925 2316 60-61 -1.247081310577073 2316 62-63 -1.0069466643446852 2316 64-65 -0.9441888130205918 2316 66-67 -0.6788102408552135 2316 68-69 -0.7724413564731982 2316 70-71 -0.845829537509502 2316 72-73 -0.9602318390966111 2316 74-75 -0.8796012021661248 2316 76-77 -0.5394349211939797 2316 78-79 -0.5989100672874983 2316 80-81 -0.5945156287196305 2316 82-83 -0.7853847449251177 2316 84-85 -0.5466276560362004 2316 86-87 -0.3991893047323174 2316 88-89 -0.6847438191372852 2316 90-91 -0.8139220076093316 2316 92-93 -0.7279238454181787 2316 94-95 -0.9106301151006448 2316 96-97 -0.5783386053003969 2316 98-99 -0.5982479442868414 2316 100-101 -0.04953680828170981 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7592.0 3 3024.0 4 3217.0 5 3702.0 6 4513.0 7 5844.0 8 7634.0 9 9613.0 10 12310.0 11 15229.0 12 18812.0 13 22226.0 14 25257.0 15 29247.0 16 33659.0 17 37991.0 18 37340.0 19 37316.0 20 40351.0 21 44261.0 22 48829.0 23 53821.0 24 59668.0 25 66069.0 26 73348.0 27 80587.0 28 89496.0 29 98695.0 30 110559.0 31 124305.0 32 137934.0 33 155687.0 34 174707.0 35 187813.0 36 178328.0 37 118586.0 38 28040.0 39 342.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.9451172270569 18.259645152268188 14.321398375432445 23.473839245242466 2 15.778084366927725 19.790100831222674 39.522498838850794 24.909315962998807 3 19.324874587794035 23.795873501391636 29.587167089054795 27.29208482175953 4 13.016402595857246 16.052255814592087 36.101042553386236 34.83029903616443 5 13.912971432965794 37.4874577500119 33.52996590705185 15.069604909970447 6 33.01931052601948 35.338917147686686 16.916174585207486 14.72559774108635 7 29.283250866315562 32.69977429115891 19.858488061961012 18.15848678056451 8 27.510094656136403 33.72697496232381 19.37231215335731 19.390618228182475 9 27.22537048893144 16.13667326727236 19.574006945966712 37.06394929782949 10-11 25.54200199394403 26.053759605037076 26.732968242196698 21.671270158822196 12-13 26.643003968341134 23.4735706284183 27.017301088731227 22.86612431450934 14-15 23.128214327061368 24.77066424201712 25.51050572155365 26.590615709367864 16-17 22.598830633057815 27.970020901816373 26.48255100994941 22.948597455176404 18-19 22.79848107440397 26.219312557356094 28.817442248086756 22.164764120153173 20-21 23.18235588880677 25.404069641063426 29.065167798902163 22.348406671227643 22-23 22.968059138963202 25.446384035335473 29.079486515353558 22.506070310347763 24-25 22.7473835308838 25.69875291383026 29.051325287916292 22.502538267369655 26-27 22.03167452496679 26.03787452011589 28.883016964867153 23.04743399005017 28-29 22.415999491138276 26.54235553097684 28.621481240643316 22.420163737241563 30-31 22.37564547749318 25.936663121612447 28.46784556499073 23.219845835903648 32-33 21.979997446599377 26.041322990993088 28.546698631459634 23.4319809309479 34-35 23.088143234472042 26.097477490339926 28.275592554560415 22.538786720627616 36-37 23.275570733158297 25.499173430805577 28.301889382733 22.92336645330313 38-39 22.52206519369398 25.900503082527177 28.335206557573567 23.24222516620528 40-41 22.98675558499979 25.797808092324754 28.473319009770893 22.74211731290457 42-43 22.74544800281288 25.868301198515876 28.125192304480816 23.261058494190433 44-45 22.620908476375337 26.109482463795796 28.44864956987316 22.8209594899557 46-47 23.234556501741707 26.45402720385596 27.57182684595143 22.739589448450904 48-49 22.83532622956783 26.599679852412738 27.320755442154383 23.24423847586505 50-51 23.108317888151415 26.75650722175682 27.37704438009651 22.758130509995254 52-53 23.4300972870392 26.298588332555184 27.475713697161407 22.795600683244206 54-55 22.645782378470077 26.66251250138396 27.704508001343243 22.987197118802722 56-57 22.812944714642605 26.795437612361756 27.29744116584692 23.094176507148717 58-59 22.75523450179163 26.127389365500726 27.480839899215752 23.63653623349189 60-61 22.241592284711707 26.57906294749267 27.87346908897927 23.305875678816353 62-63 22.53507981101659 26.560345007044674 27.98748864491355 22.917086537025188 64-65 22.259772837332765 26.64198604578069 27.647251646486705 23.45098947039984 66-67 22.0865251351462 26.65142790742465 27.66155040536426 23.600496552064882 68-69 22.9611170560024 26.061445217051567 27.78560602282808 23.191831704117956 70-71 22.67598284363011 26.237720817116838 27.319613453233018 23.76668288602004 72-73 22.58636560784881 26.444680625225324 27.345212626755266 23.623741140170598 74-75 22.574606635777968 26.90727488759879 27.20367919249557 23.314439284127673 76-77 22.74874469909721 26.341200747267457 27.402307726971507 23.507746826663826 78-79 22.747062101451863 26.571957583360557 27.24424521421977 23.436735100967816 80-81 23.05354030907275 26.528767227326362 26.896727316981888 23.520965146618998 82-83 22.88948754011385 26.51295676259829 27.104678965655133 23.492876731632727 84-85 22.755534103620473 26.657165797787048 27.036438043431016 23.550862055161463 86-87 23.105450374914994 26.619364923895034 26.579312689010067 23.695872012179905 88-89 22.896995285960507 26.63386559016544 26.438038267770068 24.03110085610398 90-91 22.719943076652047 26.534150576897996 26.609514527122098 24.13639181932786 92-93 22.984568218247883 26.030960556870024 26.91732893996088 24.067142284921214 94-95 22.652097261350185 25.72916042284281 27.1627957627867 24.455946553020304 96-97 23.030358075533606 25.364047767807403 27.054186615103287 24.5514075415557 98-99 22.779130511168514 25.607609102446904 26.52525646269521 25.088003923689367 100-101 23.650609202830807 24.666405284200756 26.779664199869647 24.90332131309879 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 343.0 1 258.0 2 241.0 3 310.5 4 286.5 5 295.0 6 478.0 7 963.5 8 1560.0 9 1859.5 10 1658.5 11 1400.5 12 1459.5 13 1579.5 14 1702.0 15 1855.5 16 2058.0 17 2322.5 18 2575.0 19 2965.5 20 3452.5 21 3998.5 22 4860.0 23 5905.0 24 7194.5 25 8992.5 26 11498.0 27 14574.0 28 17305.5 29 19991.5 30 23344.0 31 28570.5 32 35687.5 33 38160.5 34 37632.0 35 39905.0 36 43719.5 37 47869.5 38 51563.0 39 54592.5 40 57610.0 41 61267.0 42 63716.0 43 65637.5 44 68230.0 45 72349.0 46 75395.5 47 77920.0 48 80181.5 49 79620.5 50 78015.5 51 76600.0 52 76414.0 53 77712.0 54 79044.0 55 76955.5 56 72834.5 57 67890.5 58 63767.5 59 57478.0 60 47286.0 61 39568.0 62 34150.0 63 27259.5 64 19990.0 65 15046.5 66 11618.5 67 8981.5 68 6944.5 69 5510.5 70 4379.5 71 3151.0 72 2297.5 73 1786.5 74 1359.0 75 991.5 76 728.5 77 520.5 78 336.5 79 216.0 80 137.5 81 82.0 82 51.0 83 33.5 84 18.0 85 11.0 86 10.5 87 9.0 88 7.0 89 3.0 90 2.0 91 3.5 92 3.5 93 2.5 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3893498118897396 2 0.028225688395719577 3 0.03467596726735079 4 0.042635885874895696 5 0.06111753597517238 6 0.05420979051690064 7 0.03824420664314678 8 0.040485792917685295 9 0.03655158027257689 10-11 0.03822133331381476 12-13 0.030009808083617574 14-15 0.02831718171304768 16-17 0.022690342697369383 18-19 0.02705914859978627 20-21 0.034172754022046226 22-23 0.03321207419010115 24-25 0.04110337280964998 26-27 0.03609411368593638 28-29 0.03122209453821493 30-31 0.03421850068071028 32-33 0.028866141617016297 34-35 0.03055876798758619 36-37 0.03268598761546457 38-39 0.01493628405381271 40-41 0.012626077791278126 42-43 0.013289404341906869 44-45 0.01187125792332128 46-47 0.020723236374815185 48-49 0.018184296818960343 50-51 0.012122864545973562 52-53 0.009309445038134415 54-55 0.008874851780825928 56-57 0.009629671648782773 58-59 0.004323059243752836 60-61 0.0074109587035762915 62-63 0.011779764605993179 64-65 0.007388085374244266 66-67 0.0035911127051280176 68-69 0.0061757989196469095 70-71 0.013335151000570918 72-73 0.011162184714028488 74-75 0.011665397959333051 76-77 0.005558219027682218 78-79 0.009423811684794543 80-81 0.011573904642004949 82-83 0.013632504281887252 84-85 0.00855462517017757 86-87 0.0025846862145188915 88-89 0.016148570508410065 90-91 0.025480888875876507 92-93 0.010773338115384053 94-95 0.02099771632679949 96-97 0.01928221662689757 98-99 0.011619651300669 100-101 0.12301276514763361 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2185952.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.90576520362598 #Duplication Level Percentage of deduplicated Percentage of total 1 74.05048188082736 36.95545961959931 2 13.66843017004997 13.642669335373428 3 5.051475585524466 7.562932635090994 4 2.4076605838168175 4.806245751439485 5 1.2962158406588586 3.2344321698570835 6 0.8157067918338472 2.4425082976957797 7 0.5350591857872806 1.8691776667160325 8 0.36162145313849675 1.4437596266339083 9 0.26152227234391673 1.1746322207202815 >10 1.3917051754702419 12.598810489615273 >50 0.09883922268273698 3.4081186857509835 >100 0.052966211916824796 5.02878393923979 >500 0.0039730212868829395 1.346759680801004 >1k 0.004157812974644894 3.905819795331536 >5k 1.847916877619953E-4 0.579890086135286 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7063 0.3231086501441935 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5513 0.2522013292149141 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 3870 0.17703956902987805 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 3750 0.1715499699901919 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 3719 0.17013182357160633 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3703 0.1693998770329815 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 3307 0.15128420020201724 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2919 0.1335344966403654 No Hit CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG 2877 0.13161313697647523 No Hit GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA 2774 0.12690123113407797 No Hit GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA 2687 0.12292127183030552 No Hit CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG 2537 0.11605927303069782 No Hit GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA 2396 0.10960899415906664 No Hit GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT 2268 0.10375342185006808 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 4.5746658664051176E-5 0.0 7 1.3723997599215354E-4 0.0 0.0 4.5746658664051176E-5 0.0 8 1.3723997599215354E-4 0.0 0.0 4.5746658664051176E-5 4.5746658664051176E-5 9 1.3723997599215354E-4 0.0 0.0 4.5746658664051176E-5 4.5746658664051176E-5 10-11 1.3723997599215354E-4 0.0 0.0 4.5746658664051176E-5 4.5746658664051176E-5 12-13 1.829866346562047E-4 0.0 0.0 4.5746658664051176E-5 2.287332933202559E-4 14-15 2.287332933202559E-4 0.0 0.0 4.5746658664051176E-5 2.973532813163326E-4 16-17 2.516066226522815E-4 0.0 0.0 4.5746658664051176E-5 3.202266106483582E-4 18-19 3.88846598644435E-4 0.0 0.0 6.861998799607676E-5 3.202266106483582E-4 20-21 4.574665866405118E-4 0.0 0.0 1.601133053241791E-4 3.659732693124094E-4 22-23 4.574665866405118E-4 0.0 0.0 4.345932573084862E-4 3.659732693124094E-4 24-25 5.489599039686142E-4 0.0 0.0 9.835531612771005E-4 4.117199279764606E-4 26-27 6.17579891964691E-4 0.0 0.0 0.002012852981218252 4.345932573084862E-4 28-29 9.378065026130492E-4 0.0 0.0 0.004826272489057399 4.574665866405118E-4 30-31 0.001006426490609126 0.0 0.0 0.015508117287113349 4.574665866405118E-4 32-33 0.001006426490609126 0.0 0.0 0.03620848033259651 4.574665866405118E-4 34-35 0.0010750464786052029 0.0 0.0 0.06969503447468198 4.574665866405118E-4 36-37 0.0010979198079372283 0.0 0.0 0.12831937755266354 4.574665866405118E-4 38-39 0.0011436664666012795 0.0 0.0 0.217205135336915 4.574665866405118E-4 40-41 0.0011436664666012795 0.0 0.0 0.312884271932778 4.803399159725374E-4 42-43 0.0013037797719254586 0.0 0.0 0.4109193614498397 5.03213245304563E-4 44-45 0.0015096397359136888 0.0 0.0 0.5356704996267073 5.489599039686142E-4 46-47 0.0016926263705698936 0.0 0.0 0.6835008270995886 6.17579891964691E-4 48-49 0.0018298663465620472 0.0 0.0 0.8333897542123523 6.404532212967164E-4 50-51 0.0018756130052260983 0.0 0.0 1.0030869845266501 6.404532212967164E-4 52-53 0.0018984863345581238 0.0 0.0 1.1650072828680593 6.861998799607676E-4 54-55 0.0019213596638901495 0.0 0.0 1.343556491633851 6.861998799607676E-4 56-57 0.0021043462985463543 0.0 0.0 1.549942542196718 6.861998799607676E-4 58-59 0.0021043462985463543 0.0 0.0 1.7707387902387608 7.319465386248188E-4 60-61 0.0021500929572104057 0.0 0.0 2.017473393743321 7.7769319728887E-4 62-63 0.0022415862745385077 0.0 0.0 2.2610286044707295 7.7769319728887E-4 64-65 0.0022415862745385077 0.0 0.0 2.5141448668589246 7.7769319728887E-4 66-67 0.00233307959186661 0.0 0.0 2.8015253765864943 8.234398559529212E-4 68-69 0.00233307959186661 0.0 0.0 3.123375993617426 8.234398559529212E-4 70-71 0.0023559529211986357 0.0 0.0 3.4591335948822297 8.234398559529212E-4 72-73 0.0024016995798626867 0.0 0.0 3.8070369340223387 8.92059843948998E-4 74-75 0.002447446238526738 0.0 0.0 4.153064660157222 9.835531612771005E-4 76-77 0.0024703195678587634 0.0 0.0 4.522926395456076 0.001006426490609126 78-79 0.0024703195678587634 0.0 0.0 4.942903595321398 0.001006426490609126 80-81 0.002516066226522815 0.0 0.0 5.3751180263793525 0.001006426490609126 82-83 0.0025618128851868658 0.0 0.0 5.823801254556367 0.001006426490609126 84-85 0.002607559543850917 0.0 0.0 6.273879755822635 0.0010521731492731771 86-87 0.002653306202514968 0.0 0.0 6.74696882639692 0.0010750464786052029 88-89 0.002653306202514968 0.0 0.0 7.238219320460834 0.0010979198079372283 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4845 0.0 45.97149 1 GTATCAA 8410 0.0 40.26501 1 TATCAAC 10880 0.0 31.188244 2 ATCAACG 10995 0.0 30.645916 3 TCAACGC 11155 0.0 30.248955 4 CAACGCA 11320 0.0 29.765383 5 TAAGGTG 1645 0.0 29.756577 5 AACGCAG 11680 0.0 28.923372 6 GTAAGGT 1690 0.0 26.996418 4 AGGTAAG 1520 0.0 26.889114 2 TAAGGTA 755 0.0 25.808247 4 ACGCAGA 13510 0.0 24.82459 7 CGCAGAG 13645 0.0 24.613796 8 GTGTAAG 855 0.0 24.544378 1 GTACATG 10140 0.0 23.894129 1 TACATGG 9870 0.0 23.834698 2 GGTAAGG 1775 0.0 23.829409 3 AAGGTAA 1645 0.0 23.77461 1 GCAGAGT 14755 0.0 22.439152 9 ACATGGG 10205 0.0 22.260582 3 >>END_MODULE