FastQCFastQC Report
Thu 26 May 2016
SRR938460_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938460_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2185952
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68260.31226669204081337No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60220.2754863784749162No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40810.18669211400799285No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA40490.1852282209307432No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA39140.1790524220110963No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA35870.16409326462795157No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC35770.16363579804131106No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA32780.14995754710075976No Hit
CCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAAGG32340.1479446941195415No Hit
GCCCAGGTCTGAGGCCTCCCTTTTTTTTGTCCCCCCAACTTGATGTATGA26890.12301276514763361No Hit
CCTCAGACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGG26790.1225552985609931No Hit
GGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGACCAAA24490.11203356706826133No Hit
GTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGAGA23580.10787062112983267No Hit
TCCATGCCCTGAGTCCACCCCGGGGAAGGTGACAGCATTGCTTCTGTGTA23570.10782487447116862No Hit
GTGTAAATTATGTACTGCAAAAATTTTTTTAAATCTTCCGCCTTAATACT23480.10741315454319217No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53150.047.7368471
GTATCAA85850.038.4645161
TATCAAC113950.029.0976222
ATCAACG114300.028.9234183
TCAACGC115650.028.5440674
CAACGCA117250.028.1545525
AACGCAG119950.027.520816
TAAGGTG15300.027.008755
GTAAGGT16350.026.4362774
AGGTAAG14800.025.3560622
AAGGTAA14500.024.5758741
ACGCAGA137500.023.9736127
CGCAGAG138800.023.7148538
GGTAAGG17350.023.5442893
TACATGG95050.023.4388942
GTACATG98600.023.4193671
ACATGGG98100.022.1275753
TAAGGTA7200.021.7699834
GCAGAGT151150.021.4943669
AGAGTAC130400.021.21753910-11
Produced by FastQC (version 0.11.5)