FastQCFastQC Report
Thu 26 May 2016
SRR938459_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938459_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences281228
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT26180.9309172628614505No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18200.6471617335400458No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16480.5860013938868107No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15230.5415534726271921No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT11780.41887720995064504No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11550.41069879243887525No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9470.33673745146287No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7690.2734436115891732No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC7480.26597636081755727No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7290.25922027678609527No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7250.2577979433057875No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7250.2577979433057875No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7200.2560200264554027No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6100.2169058557469384No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6090.21655027237686147No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA4900.17423585133770464No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC4700.16712418393616568No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA4620.1642795169755501No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG4540.1614348500149345No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT4430.15752343294408805No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC4380.15574551609370332No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.1518340990228569No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC3440.1223206793064702No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3190.1134310950545465No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2990.10631942765300753No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGCGG250.00499498557.064335
CCGGGCA250.00501261857.0135579
CCGTCCA250.00501261857.0135579
CATTCCG352.781042E-454.298639
GGTATAC651.3182427E-436.573181
CCCTACT550.002564347834.5782851
TAGTACA753.0523085E-431.7024064
TTGGACC600.00392418631.7024024
GTAGGAC1951.6370905E-1129.2637583
TGGACCA856.352991E-427.972715
TTTGACG350.00840010127.1348382-83
TAGGACT1109.37944E-525.9383324
TAGGACG1858.800271E-925.7046534
CGCCGTA1251.4551915E-1124.70367894-95
GGGGCAT1351.333732E-524.6398746
TAGGCAT1201.6933348E-423.7768025
GTATTGG1000.001641445823.7725721
GCATTCC1201.7015346E-423.7598788
GGACGTG2002.0310836E-823.7598786
AGTACTT13650.022.79440712-13
Produced by FastQC (version 0.11.5)