##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938459_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 281228 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.0120222737423 31.0 31.0 34.0 28.0 34.0 2 31.03868391483067 31.0 31.0 34.0 27.0 34.0 3 31.05965622199781 31.0 31.0 34.0 27.0 34.0 4 34.69240971738234 37.0 35.0 37.0 32.0 37.0 5 34.68141507957956 37.0 35.0 37.0 32.0 37.0 6 34.73191147396419 37.0 35.0 37.0 32.0 37.0 7 34.65254526576301 37.0 35.0 37.0 32.0 37.0 8 34.666253004679476 37.0 35.0 37.0 32.0 37.0 9 36.116784246234374 39.0 35.0 39.0 32.0 39.0 10-11 35.98318268451221 39.0 35.0 39.0 30.5 39.0 12-13 35.90397293299387 38.0 35.0 39.0 30.0 39.0 14-15 36.819966361813194 39.0 36.0 41.0 31.0 41.0 16-17 36.693001763693516 39.0 36.0 41.0 30.5 41.0 18-19 36.866638457052645 39.0 36.0 41.0 31.0 41.0 20-21 36.89529492084714 39.0 36.0 41.0 31.0 41.0 22-23 36.80710846715121 39.0 36.0 41.0 31.0 41.0 24-25 36.66738553771317 39.0 36.0 41.0 30.0 41.0 26-27 36.46650760237245 39.0 36.0 41.0 30.0 41.0 28-29 36.277989744975606 39.0 35.0 40.5 30.0 41.0 30-31 36.04106810132704 39.0 35.0 40.0 29.0 41.0 32-33 35.756222708976345 38.0 35.0 40.0 28.5 41.0 34-35 35.498083405635285 38.0 35.0 40.0 27.0 41.0 36-37 35.215360846003954 38.0 34.5 40.0 26.5 41.0 38-39 35.06073186169229 38.0 34.0 40.0 25.5 41.0 40-41 34.98076649551254 38.0 34.0 40.0 25.0 41.0 42-43 34.67476211472542 38.0 34.0 40.0 24.0 41.0 44-45 34.28533787531825 38.0 33.0 40.0 23.5 41.0 46-47 33.8537787844738 38.0 33.0 40.0 20.5 41.0 48-49 33.45953994623579 38.0 33.0 40.0 16.5 41.0 50-51 32.756236932311154 37.5 32.0 39.5 14.5 40.5 52-53 32.80823744435121 37.5 32.5 39.5 12.5 40.5 54-55 32.96663916821938 38.0 33.0 40.0 8.5 41.0 56-57 32.66116816248738 38.0 32.0 40.0 7.5 41.0 58-59 32.34989581407257 37.0 31.0 40.0 2.0 41.0 60-61 31.90023752969121 37.0 31.0 40.0 2.0 41.0 62-63 31.421977896937715 36.0 30.5 39.0 2.0 41.0 64-65 30.981685678524187 36.0 29.5 39.0 2.0 40.0 66-67 30.482313283172374 35.5 29.0 39.0 2.0 40.0 68-69 29.959022572432332 35.0 28.0 38.5 2.0 40.0 70-71 29.354655297481045 34.5 27.5 38.0 2.0 40.0 72-73 28.744410229422392 34.0 26.0 37.0 2.0 39.0 74-75 28.132383688679646 34.0 26.0 36.5 2.0 39.0 76-77 27.42920690685138 33.5 25.5 36.0 2.0 39.0 78-79 26.677386319996586 33.0 23.0 35.5 2.0 37.5 80-81 25.966109348997968 32.0 20.5 35.0 2.0 37.0 82-83 25.257844169143898 31.5 18.5 35.0 2.0 36.5 84-85 24.587882429914515 31.0 13.5 34.5 2.0 36.0 86-87 23.890464320764647 31.0 7.0 34.0 2.0 35.5 88-89 23.100900337093037 30.5 2.0 34.0 2.0 35.0 90-91 22.200077872758044 29.5 2.0 34.0 2.0 35.0 92-93 21.305645241583342 29.0 2.0 34.0 2.0 35.0 94-95 20.33382877949564 28.0 2.0 34.0 2.0 35.0 96-97 19.124427510774176 25.5 2.0 33.5 2.0 35.0 98-99 17.716184021505683 23.0 2.0 33.0 2.0 34.5 100-101 14.182595616368214 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6597444017807597 1101 2 0.43244816148640197 1101 3 0.5392534868804226 1101 4 0.4676386684181324 1101 5 0.4727295028710472 1101 6 0.5747747470507178 1101 7 0.7008168330854971 1101 8 0.8262196480681041 1101 9 1.011593862989173 1101 10-11 1.1166495683588593 1101 12-13 1.050256301877198 1101 14-15 0.937544733844554 1101 16-17 0.8311096499326993 1101 18-19 1.2316213109126934 1101 20-21 1.1506190920548747 1101 22-23 1.2940525735738717 1101 24-25 0.9557309922815165 1101 26-27 0.8226845032089898 1101 28-29 1.101568404349159 1101 30-31 1.415685691826134 1101 32-33 1.39185880690615 1101 34-35 1.1014482574876325 1101 36-37 1.0861314291024016 1101 38-39 0.9712262273186525 1101 40-41 1.1955418111650786 1101 42-43 1.4319119835996403 1101 44-45 1.4390563550008437 1101 46-47 1.2438762324759267 1101 48-49 0.9257299088138211 1101 50-51 0.9797522822378539 1101 52-53 0.7456143117714262 1101 54-55 0.761970879836305 1101 56-57 0.9882078422552354 1101 58-59 0.8045613750974709 1101 60-61 1.3885084357765258 1101 62-63 1.2306791064180516 1101 64-65 1.1110353757561846 1101 66-67 1.075100822287407 1101 68-69 0.9495073431719661 1101 70-71 0.8177842110135636 1101 72-73 0.9799733198893996 1101 74-75 0.6638498865154752 1101 76-77 0.677883244509875 1101 78-79 0.7770007287757359 1101 80-81 1.1827425680130084 1101 82-83 1.04772696180234 1101 84-85 0.9783152818210397 1101 86-87 1.4008206606146238 1101 88-89 0.6479781415878385 1101 90-91 0.2481572016257232 1101 92-93 -0.2293391729260712 1101 94-95 -0.20123228743027965 1101 96-97 0.08328408622659822 1101 98-99 0.7921457574234232 1101 100-101 1.3844431915457172 1102 1 0.08597890696263377 1102 2 0.08221446914744845 1102 3 0.2569937835680882 1102 4 0.3886912999970775 1102 5 0.16283314034310337 1102 6 0.44949533229761585 1102 7 0.37476521756019565 1102 8 0.12168804879966189 1102 9 0.3010675471996933 1102 10-11 0.3395209359670659 1102 12-13 0.39921992715661503 1102 14-15 0.9371383123855068 1102 16-17 0.5134404770003727 1102 18-19 0.5057525850439717 1102 20-21 0.5928564421869638 1102 22-23 0.6338209133422126 1102 24-25 0.4874318660876611 1102 26-27 0.44364568995965215 1102 28-29 0.023789497622892952 1102 30-31 0.16781940448615984 1102 32-33 0.6310886382412448 1102 34-35 0.2541103180444324 1102 36-37 0.44388391843383346 1102 38-39 0.44872063902359116 1102 40-41 0.8083745687346777 1102 42-43 0.8371649809579012 1102 44-45 0.6563902302294551 1102 46-47 1.0827301239613973 1102 48-49 0.9816128594336178 1102 50-51 1.1430321034124162 1102 52-53 0.8582946612938827 1102 54-55 0.83568557197205 1102 56-57 1.0295612257138842 1102 58-59 0.6257359331141359 1102 60-61 0.20876854551032054 1102 62-63 0.8331481167817998 1102 64-65 0.9317019069490335 1102 66-67 0.5114450612632275 1102 68-69 1.5898243519100248 1102 70-71 1.0625515347282537 1102 72-73 1.3651084550245365 1102 74-75 1.404654601004399 1102 76-77 1.6274869835872927 1102 78-79 1.1753445613300961 1102 80-81 1.3530331593562295 1102 82-83 1.1351075754987399 1102 84-85 1.6393089822884228 1102 86-87 0.9104223675847543 1102 88-89 0.5414957193159395 1102 90-91 0.558053564153667 1102 92-93 0.713812625488881 1102 94-95 1.5856504599787833 1102 96-97 1.6412499468240362 1102 98-99 0.8945639651047905 1102 100-101 0.5565118767722979 1103 1 0.7169040458969249 1103 2 0.43360926047358817 1103 3 0.6627636429992734 1103 4 0.3356510907783772 1103 5 0.5144731098320463 1103 6 0.48185884978699534 1103 7 0.3289713851530891 1103 8 0.6404577992627765 1103 9 0.6767077346247774 1103 10-11 0.5433184732886289 1103 12-13 0.7070955945860575 1103 14-15 0.9215831200568587 1103 16-17 0.8492255919616838 1103 18-19 0.4964358148576338 1103 20-21 0.24885785877190614 1103 22-23 0.8592349920171571 1103 24-25 1.0695256875979524 1103 26-27 0.9388783367941258 1103 28-29 0.9293550419184058 1103 30-31 0.9949551787043163 1103 32-33 1.221205234078667 1103 34-35 1.1523477594740967 1103 36-37 1.0083657752497928 1103 38-39 1.0419973089592958 1103 40-41 1.2285282137167002 1103 42-43 1.4673954484309277 1103 44-45 1.5093378165866511 1103 46-47 1.2779328045759755 1103 48-49 1.386238554105077 1103 50-51 1.6000169568503537 1103 52-53 1.9211011339973751 1103 54-55 1.6301581665018574 1103 56-57 1.6261478008473702 1103 58-59 1.5632836066457898 1103 60-61 1.8472124814370936 1103 62-63 1.6335594712549337 1103 64-65 1.6654111801319864 1103 66-67 1.9561301435341214 1103 68-69 1.998427381222438 1103 70-71 2.159573982151162 1103 72-73 1.9906260533682207 1103 74-75 1.9645325567633627 1103 76-77 1.5203305054353926 1103 78-79 1.7510228875805076 1103 80-81 2.1131535690761787 1103 82-83 2.1148258930317034 1103 84-85 1.9959206200785502 1103 86-87 1.9018138898687234 1103 88-89 1.4533541697899537 1103 90-91 1.479187921786881 1103 92-93 2.10292399085883 1103 94-95 1.9086454474474586 1103 96-97 2.622684511496587 1103 98-99 2.726480713497512 1103 100-101 2.316427971356582 1104 1 0.8701421121784705 1104 2 0.788403017441258 1104 3 0.7149831626100998 1104 4 1.0065997073791664 1104 5 0.5652990954406434 1104 6 0.9804906527666262 1104 7 0.9087575410262048 1104 8 1.035537357385813 1104 9 1.3057996571949673 1104 10-11 1.0965007982100872 1104 12-13 0.9637212153421117 1104 14-15 1.4403324866323075 1104 16-17 1.397543216366266 1104 18-19 1.2430153223067038 1104 20-21 1.1736703651061546 1104 22-23 1.095109624630922 1104 24-25 1.0672073537578797 1104 26-27 0.9466821272066142 1104 28-29 1.0530628558436135 1104 30-31 0.9932702694107078 1104 32-33 0.90117381622116 1104 34-35 0.9283308343702146 1104 36-37 1.0351216280926039 1104 38-39 1.067562323654748 1104 40-41 0.6909788066020752 1104 42-43 0.6060014076890567 1104 44-45 0.19249885844335068 1104 46-47 0.47347096207065675 1104 48-49 0.5615197946037114 1104 50-51 0.5232965757821546 1104 52-53 0.5704246365817554 1104 54-55 0.7519403698057943 1104 56-57 0.915280769328163 1104 58-59 1.0943594148955142 1104 60-61 0.9462547435228323 1104 62-63 1.0300959058348411 1104 64-65 0.7831267978476113 1104 66-67 1.2980400452266316 1104 68-69 1.3085267021913225 1104 70-71 1.5470144402437924 1104 72-73 1.944778784694865 1104 74-75 0.8786148512804424 1104 76-77 1.2598283264549544 1104 78-79 1.125604827379835 1104 80-81 1.2851536704241084 1104 82-83 1.4491093631847392 1104 84-85 1.1090629125686675 1104 86-87 1.2417095015034683 1104 88-89 1.6825651761748759 1104 90-91 1.7922009956695177 1104 92-93 1.9490905055036016 1104 94-95 1.7632491770511862 1104 96-97 2.241780994723502 1104 98-99 2.021200486478154 1104 100-101 1.6193569264594512 1105 1 0.6002071859935434 1105 2 0.5952940430822835 1105 3 0.5430474594243968 1105 4 0.4185315255609865 1105 5 0.38962407601562177 1105 6 0.7080061927821646 1105 7 0.8907165542352189 1105 8 0.7043164949149556 1105 9 1.0527644979098056 1105 10-11 1.141069077153368 1105 12-13 1.1642966815425808 1105 14-15 1.0602455596703848 1105 16-17 1.157498538821585 1105 18-19 1.091318061234439 1105 20-21 1.2599538732646565 1105 22-23 1.1444855958818962 1105 24-25 1.2129435619940878 1105 26-27 1.2617195203689988 1105 28-29 1.1253846312903946 1105 30-31 1.0002389951294361 1105 32-33 1.535667794465141 1105 34-35 1.6096998126141955 1105 36-37 1.566638722109694 1105 38-39 1.4922539514713762 1105 40-41 1.6865596146828779 1105 42-43 1.5207619943246513 1105 44-45 1.722049169243661 1105 46-47 1.413726886701582 1105 48-49 1.45900425458818 1105 50-51 1.3686612805218559 1105 52-53 1.2174477523548646 1105 54-55 1.2662541809946077 1105 56-57 0.8575034771758752 1105 58-59 1.3517045003655994 1105 60-61 1.8051683993114942 1105 62-63 1.87102927801822 1105 64-65 1.622008402354215 1105 66-67 1.7948227763843612 1105 68-69 1.9266429609325826 1105 70-71 1.7841210836004358 1105 72-73 1.7373802804213625 1105 74-75 1.9399250688406582 1105 76-77 2.3265654088170358 1105 78-79 2.3463580325080393 1105 80-81 2.7837453565157944 1105 82-83 2.77394425051963 1105 84-85 2.8540124075181623 1105 86-87 3.2026894937334625 1105 88-89 3.177538952398649 1105 90-91 3.0185146126081364 1105 92-93 2.7307168652549656 1105 94-95 2.66211766966968 1105 96-97 3.2986355984531066 1105 98-99 3.2242162207438874 1105 100-101 2.3340155317430558 1106 1 -0.058458272943653355 1106 2 -0.0011733482220641633 1106 3 0.04682161401376561 1106 4 -0.16434890922162282 1106 5 -0.17755587567520337 1106 6 -0.079735353111559 1106 7 0.13694239964584654 1106 8 0.04263171230626028 1106 9 -0.5080748740708714 1106 10-11 0.08679673415819167 1106 12-13 0.009631826470112514 1106 14-15 -0.022257459653289402 1106 16-17 0.01951544703415209 1106 18-19 0.08882711920545461 1106 20-21 -0.10802619919910939 1106 22-23 0.11648136882875093 1106 24-25 -0.05909750080784448 1106 26-27 -0.14988977914790524 1106 28-29 -0.01086234938593833 1106 30-31 -0.30576945897684027 1106 32-33 -0.26067882389234853 1106 34-35 -0.4509131100911219 1106 36-37 -0.05141683344586312 1106 38-39 -0.5413360243740328 1106 40-41 -0.5160370036746116 1106 42-43 -0.5235465805545729 1106 44-45 -0.2877636326887085 1106 46-47 0.11126613790931117 1106 48-49 0.32283275700363134 1106 50-51 0.30472710177789963 1106 52-53 -0.02455103991566432 1106 54-55 -0.3698418334981426 1106 56-57 -0.8919681411816143 1106 58-59 0.17741404142839912 1106 60-61 -0.13285998233102347 1106 62-63 0.21689280458826943 1106 64-65 0.20707784679865782 1106 66-67 -0.7485800013934174 1106 68-69 -0.4907030535601713 1106 70-71 -0.2570926845155057 1106 72-73 -0.17241742489264666 1106 74-75 0.3993151654590186 1106 76-77 0.40438847644988485 1106 78-79 0.07348665569644552 1106 80-81 -0.03902034396729803 1106 82-83 0.036927342307066624 1106 84-85 -0.031253292964926516 1106 86-87 -0.12354842897185492 1106 88-89 0.27944112631169205 1106 90-91 0.47375313917818573 1106 92-93 0.5811848604240488 1106 94-95 0.5372686358532519 1106 96-97 -0.13093867690921002 1106 98-99 0.7590894091496843 1106 100-101 0.34541347860296057 1107 1 0.38311929892615026 1107 2 0.03316972614239333 1107 3 0.11361895304916914 1107 4 0.41658480424950994 1107 5 0.32055531463128517 1107 6 0.4164989068076679 1107 7 0.25183904968147885 1107 8 0.40202824354883404 1107 9 0.6195563922631777 1107 10-11 0.638923129976682 1107 12-13 0.6359326105043266 1107 14-15 0.7160117254263056 1107 16-17 0.7097981745228665 1107 18-19 0.6748392360978315 1107 20-21 0.6591512776362478 1107 22-23 0.38393800936094635 1107 24-25 0.43764756928005966 1107 26-27 0.4522306770174538 1107 28-29 0.7305132742776408 1107 30-31 0.9574225106121261 1107 32-33 1.1092902899113994 1107 34-35 0.7126768162571793 1107 36-37 0.6088290681934794 1107 38-39 0.9649600079376768 1107 40-41 1.0333036948286036 1107 42-43 0.6872635881315716 1107 44-45 0.6206944333602351 1107 46-47 1.02852083023528 1107 48-49 1.0410853110544736 1107 50-51 0.5656501238734037 1107 52-53 0.5532287177464852 1107 54-55 0.9106701646011643 1107 56-57 0.7060064371030137 1107 58-59 0.8274615586533898 1107 60-61 0.3281818328269672 1107 62-63 0.6195834674535554 1107 64-65 0.6460122730281661 1107 66-67 0.2863926762022153 1107 68-69 0.7410384527358609 1107 70-71 1.385453050813549 1107 72-73 1.1031646611135315 1107 74-75 0.7982697865091453 1107 76-77 1.1560337603653075 1107 78-79 1.8058696445298992 1107 80-81 2.0135040086105143 1107 82-83 1.705661008260975 1107 84-85 1.6653967426729892 1107 86-87 1.5195496940977584 1107 88-89 1.322723378628158 1107 90-91 0.7239847856500319 1107 92-93 0.7155813916666212 1107 94-95 1.675721950179696 1107 96-97 2.4567127842454575 1107 98-99 2.137433433858643 1107 100-101 2.670360021724843 1108 1 0.06136841165219309 1108 2 0.08723991937094411 1108 3 0.28270238496849487 1108 4 0.4347471612998177 1108 5 0.1753127930832008 1108 6 0.23241913665386704 1108 7 0.16996197233372357 1108 8 0.12667474233763443 1108 9 0.36014565483993266 1108 10-11 0.44044980453022475 1108 12-13 0.3044921282779782 1108 14-15 0.4844473065203303 1108 16-17 0.5046491688330406 1108 18-19 0.5334894529870198 1108 20-21 0.6740969145017743 1108 22-23 0.33583367721064405 1108 24-25 0.34617666623234555 1108 26-27 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-2.4564936430868975 2316 86-87 -2.334267326948609 2316 88-89 -2.351442070703662 2316 90-91 -2.546030904443569 2316 92-93 -2.6262516440959693 2316 94-95 -2.5076086779650844 2316 96-97 -3.3434332959363307 2316 98-99 -3.259385264404628 2316 100-101 -3.2610580381139282 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2526.0 3 510.0 4 400.0 5 335.0 6 416.0 7 513.0 8 618.0 9 694.0 10 944.0 11 1254.0 12 1696.0 13 2206.0 14 2586.0 15 3145.0 16 3665.0 17 4300.0 18 3925.0 19 3265.0 20 3538.0 21 3780.0 22 3924.0 23 4451.0 24 4812.0 25 5332.0 26 6245.0 27 6986.0 28 7991.0 29 9046.0 30 10775.0 31 12371.0 32 14776.0 33 17514.0 34 21647.0 35 26306.0 36 32003.0 37 35802.0 38 19818.0 39 1113.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.363713950428505 20.98716261868355 15.236300273816722 21.41282315707123 2 15.42571747937091 22.790604953829284 38.88521042027097 22.89846714652883 3 20.104810458260943 25.22037253282447 31.603699695252203 23.071117313662384 4 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20.113763255784182 28.65347548702338 30.6495778775098 20.583183379682644 50-51 19.039279103715966 29.229513647492674 31.08776321942494 20.643444029366425 52-53 19.830832749527918 29.608714051514752 29.65618898937059 20.90426420958674 54-55 19.50693018252296 28.5452395558282 30.199592812880628 21.748237448768215 56-57 20.53960243751078 28.450487840007682 29.455685420989834 21.554224301491704 58-59 21.179244695852887 28.19951524247657 29.499077964871972 21.122162096798572 60-61 20.093683145392045 28.42273363434764 30.666647697667347 20.816935522592967 62-63 19.635096977930143 28.26051394391141 30.643532315308075 21.460856762850376 64-65 19.471416709192066 29.226417073695476 29.60710088209826 21.6950653350142 66-67 20.10280774311933 28.147486971225565 29.212192550261463 22.537512735393637 68-69 19.79754191056708 27.897465806525034 29.846831724716743 22.458160558191143 70-71 20.610634074853984 27.53971415766147 29.68744442152141 22.162207345963136 72-73 20.08013701188183 26.835228273106082 30.934558330532276 22.15007638447981 74-75 19.52411948410412 28.458216844157047 29.73497378548918 22.282689886249653 76-77 19.777069610618714 27.226280747179583 29.87153405130102 23.125115590900684 78-79 19.897374077886887 27.342020621196202 30.04048496615585 22.720120334761067 80-81 20.231815102652593 26.88169748822522 29.764362206208407 23.12212520291378 82-83 19.71132048298167 26.800824290614006 30.54658148759919 22.941273738805133 84-85 19.828032238621272 26.998511817449604 30.216862157313518 22.956593786615603 86-87 19.792064894814427 27.00720322482561 30.012429163547523 23.188302716812444 88-89 19.901785015545073 26.886752182357586 29.44190695722081 23.76955584487653 90-91 20.211186067482075 26.191589907034736 29.73287825943125 23.864345766051937 92-93 20.686705354459264 26.297976627261555 29.808511432936317 23.20680658534286 94-95 20.18488806036522 26.030011846781626 29.81742437448637 23.96767571836678 96-97 19.688941301188702 26.022528988329963 30.22452504317789 24.06400466730344 98-99 20.40291515946947 25.980878602601447 29.321432255264963 24.29477398266412 100-101 20.524778124933277 25.03184894702755 29.97911136099981 24.464261567039365 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 663.0 1 403.0 2 168.5 3 180.0 4 132.5 5 139.0 6 216.0 7 324.5 8 452.5 9 483.0 10 396.5 11 303.0 12 255.5 13 240.5 14 252.5 15 287.5 16 315.5 17 347.0 18 408.0 19 526.5 20 650.5 21 748.5 22 880.5 23 1071.5 24 1281.5 25 1591.5 26 2075.0 27 2650.5 28 3220.5 29 3762.5 30 4437.5 31 5216.0 32 5997.0 33 6832.0 34 7835.0 35 8613.0 36 9164.5 37 9713.5 38 9868.5 39 9766.5 40 9758.0 41 9765.0 42 9828.0 43 9950.5 44 9958.5 45 10091.0 46 10790.5 47 12662.0 48 15451.0 49 15373.5 50 12189.0 51 9584.5 52 7945.5 53 6765.0 54 5829.5 55 4941.0 56 4130.0 57 3388.0 58 2680.5 59 2078.0 60 1587.0 61 1197.5 62 868.0 63 632.5 64 500.5 65 387.5 66 298.5 67 247.0 68 177.5 69 127.5 70 105.5 71 86.0 72 71.5 73 65.5 74 47.0 75 27.0 76 27.5 77 25.0 78 15.0 79 9.5 80 8.5 81 6.5 82 5.5 83 3.0 84 1.5 85 1.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006400500661385068 2 0.11165317820416175 3 0.1208983458261624 4 0.12658767974739357 5 0.1710356010070121 6 0.09991892699162246 7 0.029869003086463654 8 0.02844666960615586 9 0.02809108623607891 10-11 0.054048672251696135 12-13 0.058671256062696464 14-15 0.029869003086463654 16-17 0.03324704510219466 18-19 0.040003129133656674 20-21 0.060093589543004255 22-23 0.03466937858250246 24-25 0.049426088440695806 26-27 0.05920463111781188 28-29 0.05671554752727325 30-31 0.07182784075554353 32-33 0.05387088056665766 34-35 0.06756084031462017 36-37 0.05707113089735019 38-39 0.03466937858250246 40-41 0.038403003968310416 42-43 0.03324704510219466 44-45 0.006578292346423542 46-47 0.013867751433000982 48-49 0.010667501102308448 50-51 0.008000625826731335 52-53 0.009600750992077604 54-55 0.010845292787346921 56-57 0.014045543118039456 58-59 0.020090460409347574 60-61 0.024001877480194007 62-63 0.010667501102308448 64-65 0.008356209196808283 66-67 0.008356209196808283 68-69 0.01208983458261624 70-71 0.03342483678723313 72-73 0.030046794771502128 74-75 0.03058016982661755 76-77 0.02275733568492469 78-79 0.004444792125961852 80-81 0.004444792125961852 82-83 0.0058671256062696455 84-85 0.004444792125961852 86-87 0.012267626267654716 88-89 0.039114170708464305 90-91 0.03609171206281025 92-93 0.03146912825180992 94-95 0.049426088440695806 96-97 0.04391454620450311 98-99 0.027379919495925015 100-101 0.07609484119646692 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 281228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.07206257329969 #Duplication Level Percentage of deduplicated Percentage of total 1 82.02590619680257 55.83672619260356 2 11.654826125771264 15.867361066288591 3 3.3872947876042314 6.9174042820802155 4 1.2652804247177498 3.4452099297663157 5 0.6337865368543807 2.1571578397433155 6 0.3341194659529735 1.3646520715984973 7 0.16338183570975853 0.7785216980642677 8 0.113019823048759 0.6154793973278705 9 0.07651111960753515 0.4687442749329615 >10 0.30409773065423185 3.379700718847827 >50 0.021410176054200224 1.0027862671006509 >100 0.01253278598294646 2.292438368143245 >500 0.004699794743604923 2.339716132597122 >1k 0.003133196495736615 3.5341017609055885 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2618 0.9309172628614505 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1820 0.6471617335400458 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1648 0.5860013938868107 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1523 0.5415534726271921 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1178 0.41887720995064504 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1155 0.41069879243887525 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 947 0.33673745146287 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 769 0.2734436115891732 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 748 0.26597636081755727 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 729 0.25922027678609527 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 725 0.2577979433057875 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 725 0.2577979433057875 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 720 0.2560200264554027 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 610 0.2169058557469384 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 609 0.21655027237686147 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 490 0.17423585133770464 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 470 0.16712418393616568 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 462 0.1642795169755501 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 454 0.1614348500149345 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 443 0.15752343294408805 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 438 0.15574551609370332 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 427 0.1518340990228569 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 344 0.1223206793064702 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 319 0.1134310950545465 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 299 0.10631942765300753 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.889584251923706E-4 0.0 22-23 0.0 0.0 0.0 0.004089208755884905 0.0 24-25 0.0 0.0 0.0 0.009245167622000654 0.0 26-27 0.0 0.0 0.0 0.023468502425078586 0.0 28-29 0.0 0.0 0.0 0.052981922141465285 0.0 30-31 0.0 0.0 0.0 0.1264098880623551 0.0 32-33 0.0 0.0 0.0 0.25993144352624914 0.0 34-35 0.0 0.0 0.0 0.4663475898559176 0.0 36-37 0.0 0.0 0.0 0.7589927034292461 0.0 38-39 0.0 0.0 0.0 1.0880851124354618 0.0 40-41 0.0 0.0 0.0 1.3814413927489437 0.0 42-43 0.0 0.0 0.0 1.6845762157395423 0.0 44-45 0.0 0.0 0.0 2.0828295902257246 0.0 46-47 0.0 0.0 0.0 2.533887095168333 0.0 48-49 0.0 0.0 0.0 2.9543644302843246 0.0 50-51 0.0 0.0 0.0 3.324348926849389 0.0 52-53 0.0 0.0 0.0 3.6825991722019147 0.0 54-55 0.0 0.0 0.0 4.123700342782369 0.0 56-57 0.0 0.0 0.0 4.677521441677216 0.0 58-59 0.0 0.0 0.0 5.180494118651058 0.0 60-61 0.0 0.0 0.0 5.701957130868903 0.0 62-63 0.0 0.0 0.0 6.200485015716785 0.0 64-65 0.0 0.0 3.555833700769482E-4 6.757328573257286 0.0 66-67 0.0 0.0 3.555833700769482E-4 7.416757933064987 0.0 68-69 0.0 0.0 3.555833700769482E-4 8.138414382636153 0.0 70-71 0.0 0.0 3.555833700769482E-4 8.84015816348301 0.0 72-73 0.0 0.0 3.555833700769482E-4 9.533901318503137 0.0 74-75 0.0 0.0 3.555833700769482E-4 10.25573555975934 0.0 76-77 0.0 0.0 3.555833700769482E-4 11.040152474149089 0.0 78-79 0.0 0.0 3.555833700769482E-4 11.933200108097344 0.0 80-81 0.0 0.0 3.555833700769482E-4 12.852560911431294 0.0 82-83 0.0 0.0 3.555833700769482E-4 13.722495626324548 0.0 84-85 0.0 0.0 3.555833700769482E-4 14.599542008619341 0.0 86-87 0.0 0.0 3.555833700769482E-4 15.491700684142405 0.0 88-89 0.0 0.0 3.555833700769482E-4 16.46422120130286 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGCGG 25 0.004994985 57.06433 5 CCGGGCA 25 0.005012618 57.013557 9 CCGTCCA 25 0.005012618 57.013557 9 CATTCCG 35 2.781042E-4 54.29863 9 GGTATAC 65 1.3182427E-4 36.57318 1 CCCTACT 55 0.0025643478 34.578285 1 TAGTACA 75 3.0523085E-4 31.702406 4 TTGGACC 60 0.003924186 31.702402 4 GTAGGAC 195 1.6370905E-11 29.263758 3 TGGACCA 85 6.352991E-4 27.97271 5 TTTGACG 35 0.008400101 27.13483 82-83 TAGGACT 110 9.37944E-5 25.938332 4 TAGGACG 185 8.800271E-9 25.704653 4 CGCCGTA 125 1.4551915E-11 24.703678 94-95 GGGGCAT 135 1.333732E-5 24.639874 6 TAGGCAT 120 1.6933348E-4 23.776802 5 GTATTGG 100 0.0016414458 23.772572 1 GCATTCC 120 1.7015346E-4 23.759878 8 GGACGTG 200 2.0310836E-8 23.759878 6 AGTACTT 1365 0.0 22.794407 12-13 >>END_MODULE