##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938459_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 281228 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.712425505283967 31.0 31.0 34.0 30.0 34.0 2 31.91788868818183 31.0 31.0 34.0 30.0 34.0 3 31.97791827271822 31.0 31.0 34.0 30.0 34.0 4 35.65907733227132 37.0 35.0 37.0 33.0 37.0 5 35.55880282190962 37.0 35.0 37.0 33.0 37.0 6 35.56873782126957 37.0 35.0 37.0 33.0 37.0 7 35.51363306640875 37.0 35.0 37.0 33.0 37.0 8 35.51493094570953 37.0 35.0 37.0 33.0 37.0 9 37.04963943846274 39.0 37.0 39.0 33.0 39.0 10-11 36.972851209694625 39.0 37.0 39.0 33.0 39.0 12-13 36.89988728007169 39.0 37.0 39.0 33.0 39.0 14-15 37.86798078427468 40.0 37.0 41.0 32.5 41.0 16-17 37.807227587580186 40.0 37.0 41.0 32.5 41.0 18-19 38.07309905130357 40.0 37.0 41.0 33.0 41.0 20-21 38.065678026370065 40.0 37.0 41.0 33.0 41.0 22-23 37.96031867381626 40.0 37.0 41.0 33.0 41.0 24-25 37.82519343735332 40.0 37.0 41.0 32.0 41.0 26-27 37.621083249178604 39.5 36.5 41.0 32.0 41.0 28-29 37.50749569744122 39.0 36.0 41.0 32.0 41.0 30-31 37.269658426614704 39.0 36.0 41.0 31.5 41.0 32-33 37.021804372253115 39.0 35.5 41.0 31.0 41.0 34-35 36.849879812820916 39.0 35.0 41.0 31.0 41.0 36-37 36.77994011976048 39.0 35.0 41.0 30.5 41.0 38-39 36.58319584109691 39.0 35.0 41.0 30.0 41.0 40-41 36.4530718847341 39.0 35.0 40.5 30.0 41.0 42-43 36.19100871890423 39.0 35.0 40.0 30.0 41.0 44-45 35.858278692022125 39.0 35.0 40.0 29.5 41.0 46-47 35.409152004779045 38.0 35.0 40.0 27.5 41.0 48-49 35.036267725830996 38.0 34.0 40.0 26.0 41.0 50-51 34.64377124610636 38.0 34.0 40.0 24.0 41.0 52-53 34.23365383247756 38.0 33.0 40.0 23.0 41.0 54-55 33.95722332057974 38.0 33.0 40.0 22.5 41.0 56-57 33.60104612627477 38.0 33.0 40.0 20.5 41.0 58-59 33.16412306029272 37.0 32.0 40.0 18.5 41.0 60-61 32.71501237430128 37.0 31.5 40.0 14.0 41.0 62-63 32.27369963161563 36.5 31.0 39.0 10.0 40.5 64-65 31.79335450239663 36.0 31.0 39.0 8.0 40.0 66-67 31.28037926522252 35.5 30.0 39.0 4.5 40.0 68-69 30.79532621218371 35.0 29.0 38.0 2.0 40.0 70-71 30.262683658810644 34.5 29.0 38.0 2.0 40.0 72-73 29.792550883980258 34.0 28.5 37.0 2.0 39.0 74-75 29.25041247670929 34.0 28.0 36.5 2.0 39.0 76-77 27.98553842433897 32.5 26.5 35.0 2.0 37.5 78-79 28.670381327606073 34.0 28.0 36.0 2.0 38.0 80-81 28.31060029584536 34.0 27.0 35.0 2.0 37.0 82-83 27.889770933193 33.0 26.0 35.0 2.0 37.0 84-85 27.389392236903866 33.0 26.0 35.0 2.0 36.0 86-87 26.88073911559304 32.5 25.5 35.0 2.0 36.0 88-89 26.212311718605545 32.0 24.5 34.5 2.0 35.0 90-91 25.66086591662281 32.0 22.0 34.0 2.0 35.0 92-93 24.936663490121894 31.0 19.5 34.0 2.0 35.0 94-95 24.226559944244528 31.0 12.5 34.0 2.0 35.0 96-97 23.288397670217762 31.0 2.0 34.0 2.0 35.0 98-99 22.101053237942168 30.0 2.0 34.0 2.0 35.0 100-101 18.352644473523263 24.0 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36561395308260103 1101 2 0.34738935931085635 1101 3 0.3252239344979486 1101 4 0.40652299464733943 1101 5 0.38479757537498216 1101 6 0.40228040780556285 1101 7 0.29446307600523625 1101 8 0.31333016931323954 1101 9 0.4260726942191795 1101 10-11 0.6665312928769609 1101 12-13 0.7119425685894925 1101 14-15 0.693287929261146 1101 16-17 0.8555779368312884 1101 18-19 0.592162782778999 1101 20-21 0.5889837992310873 1101 22-23 0.7873796527194301 1101 24-25 0.45115553020232113 1101 26-27 0.6321749203379383 1101 28-29 0.5812506876590433 1101 30-31 0.45728157498853506 1101 32-33 0.801944493184763 1101 34-35 0.6363611753437581 1101 36-37 0.4146455670167697 1101 38-39 0.39574559065087556 1101 40-41 0.47243208849688756 1101 42-43 0.3407412620461443 1101 44-45 0.6648344390685708 1101 46-47 0.3004805415302556 1101 48-49 0.3399552847015812 1101 50-51 0.10030262372129783 1101 52-53 0.22600302422483765 1101 54-55 0.04012290120748929 1101 56-57 0.15919358985831167 1101 58-59 0.12639788002350372 1101 60-61 0.07232293174945426 1101 62-63 0.6387187260768883 1101 64-65 0.03898639402573778 1101 66-67 0.20178038200897674 1101 68-69 0.8708079549957048 1101 70-71 0.7579725127836205 1101 72-73 0.20218301974942676 1101 74-75 0.08744481647607572 1101 76-77 0.09401501530559386 1101 78-79 0.6314976114959272 1101 80-81 0.6465562816616668 1101 82-83 0.42319218222311505 1101 84-85 -0.1971480624148576 1101 86-87 0.07309910782455376 1101 88-89 0.15336970548297657 1101 90-91 0.7071400647156878 1101 92-93 0.7108772174556144 1101 94-95 0.7590331272957513 1101 96-97 0.8258795018145868 1101 98-99 1.2245786748758967 1101 100-101 0.9150267455588512 1102 1 -0.012662819931016145 1102 2 0.13351006648418817 1102 3 0.19242572275236824 1102 4 0.2954683309610999 1102 5 0.34466345629349604 1102 6 0.2483482801892265 1102 7 -0.11825791566312205 1102 8 0.03980852737054619 1102 9 0.14564188747257845 1102 10-11 0.27677311364509904 1102 12-13 0.0019242796238359006 1102 14-15 0.13862424301851206 1102 16-17 0.06778074113935872 1102 18-19 -0.021533620391089414 1102 20-21 -0.09080689371750594 1102 22-23 -0.31310805303143496 1102 24-25 -0.10493063114699908 1102 26-27 -0.32550644523816175 1102 28-29 -0.06943006828486631 1102 30-31 -0.0020762791061557095 1102 32-33 -0.09411321866242872 1102 34-35 -0.08122671329675768 1102 36-37 -0.23369826553758344 1102 38-39 0.21488804137226936 1102 40-41 0.22400697165071648 1102 42-43 0.08327326773604682 1102 44-45 0.5822800801984229 1102 46-47 0.6980115311665074 1102 48-49 0.40102010892430684 1102 50-51 0.5085529793400738 1102 52-53 -0.11706748989831084 1102 54-55 0.5137055063690426 1102 56-57 0.4129529883545473 1102 58-59 0.35531476683512864 1102 60-61 0.32054483786609467 1102 62-63 0.6291678217891388 1102 64-65 0.7680556888845054 1102 66-67 0.04053266812968204 1102 68-69 -0.08138672088318089 1102 70-71 -0.07575030778130198 1102 72-73 -0.20754061365841636 1102 74-75 -0.15762732333290685 1102 76-77 -0.4773627534405982 1102 78-79 -0.367384729491679 1102 80-81 0.03072616583155252 1102 82-83 0.07163762014223707 1102 84-85 0.1537460647950617 1102 86-87 -0.2119547430187687 1102 88-89 -0.22394181656538947 1102 90-91 -0.2924027111428771 1102 92-93 0.15423222660009728 1102 94-95 0.5134529606629528 1102 96-97 -0.02150924031100132 1102 98-99 0.16625719550178886 1102 100-101 1.1983533052011985 1103 1 0.07391231355487449 1103 2 0.2836408281710199 1103 3 0.22506166042263231 1103 4 0.32567972902146636 1103 5 0.15576730286645102 1103 6 0.03681400973047033 1103 7 0.1276285396054817 1103 8 0.13586388315130193 1103 9 0.021254832879577634 1103 10-11 0.023939943763870986 1103 12-13 0.012303488515627237 1103 14-15 0.4602707505917891 1103 16-17 0.6633321195854691 1103 18-19 0.28231022836122577 1103 20-21 0.41609895786798035 1103 22-23 0.22324642010793383 1103 24-25 0.4994880241869808 1103 26-27 0.3469082872452205 1103 28-29 0.49461591624166346 1103 30-31 0.5366210075888418 1103 32-33 0.3682110703209105 1103 34-35 0.7135183014139344 1103 36-37 0.6590499092472015 1103 38-39 0.7118233143372947 1103 40-41 0.731553010661294 1103 42-43 0.7567504389248896 1103 44-45 0.9713505825412341 1103 46-47 1.061161253297918 1103 48-49 1.1925097024733944 1103 50-51 1.1523772138393653 1103 52-53 1.2064042328869178 1103 54-55 1.029015520534891 1103 56-57 1.3952184345788012 1103 58-59 1.3524134919086777 1103 60-61 1.4879334180121049 1103 62-63 1.552181878670467 1103 64-65 1.722915664911529 1103 66-67 1.1791809191921239 1103 68-69 1.3124102715980221 1103 70-71 1.0798697205503949 1103 72-73 1.7975400227151255 1103 74-75 1.2485647873786547 1103 76-77 0.9820738816253254 1103 78-79 1.5841653413375703 1103 80-81 1.4970822734920048 1103 82-83 1.4336840405410634 1103 84-85 1.9331538233218133 1103 86-87 1.725007227123978 1103 88-89 1.1814738966306457 1103 90-91 1.117371724947347 1103 92-93 1.3272599044904787 1103 94-95 0.8863553252701237 1103 96-97 0.6991238641893815 1103 98-99 0.7702672205644454 1103 100-101 1.101725867040578 1104 1 0.2663213537900013 1104 2 0.1960002079217027 1104 3 0.2589118681858835 1104 4 0.12300921113355656 1104 5 0.12343731401472269 1104 6 0.014897520422678667 1104 7 0.20946699100915112 1104 8 0.15527201125508583 1104 9 0.23423750238398355 1104 10-11 0.11046573681140615 1104 12-13 0.37685198349890925 1104 14-15 0.5164175023907234 1104 16-17 0.5179358491892074 1104 18-19 0.2432279338441532 1104 20-21 0.2931512533985341 1104 22-23 0.43188379722356984 1104 24-25 0.4619892910360761 1104 26-27 0.7450282831913029 1104 28-29 0.7989384053467603 1104 30-31 0.9047800224869817 1104 32-33 0.9433098845501604 1104 34-35 0.8740154129979985 1104 36-37 0.8827247850959878 1104 38-39 0.8715603164656045 1104 40-41 1.1843755504403504 1104 42-43 0.9320823533872371 1104 44-45 1.142653916888051 1104 46-47 1.3307340717837874 1104 48-49 0.9583706531169511 1104 50-51 1.277402550821222 1104 52-53 1.3883744365962514 1104 54-55 1.092205953290538 1104 56-57 1.0115911922559135 1104 58-59 1.0140507676763875 1104 60-61 1.6016584610849804 1104 62-63 1.3582019455601042 1104 64-65 1.310302915342259 1104 66-67 0.9986929289215247 1104 68-69 1.4181795023672485 1104 70-71 1.4990827538938625 1104 72-73 1.2008667684331797 1104 74-75 0.8326455616768165 1104 76-77 1.0544869757775501 1104 78-79 1.441201421199736 1104 80-81 1.8189379540433386 1104 82-83 1.7634195224504587 1104 84-85 1.9098057945390003 1104 86-87 1.8586851434105895 1104 88-89 2.1791682312815013 1104 90-91 2.0387003962760204 1104 92-93 2.542383548961947 1104 94-95 2.5443694126320366 1104 96-97 2.5214609473960294 1104 98-99 2.396724097021316 1104 100-101 2.62549195602406 1105 1 0.31500530872395416 1105 2 0.4937857557072469 1105 3 0.3577609357849525 1105 4 0.280691806316149 1105 5 0.09432406615147215 1105 6 0.3787524155275719 1105 7 0.37098602752818977 1105 8 0.3832974580305333 1105 9 0.6556147362612208 1105 10-11 0.24510540270106418 1105 12-13 0.470334889481812 1105 14-15 0.7757900742632913 1105 16-17 0.6077765640299191 1105 18-19 0.683653827395041 1105 20-21 0.43451683226412285 1105 22-23 0.5784698500596264 1105 24-25 0.4148714782932643 1105 26-27 0.4317512824143037 1105 28-29 0.4478918824252318 1105 30-31 0.8027743892313524 1105 32-33 0.8618704906107553 1105 34-35 0.5165829149404999 1105 36-37 0.588095802967004 1105 38-39 0.4775237974290789 1105 40-41 0.7527637594535648 1105 42-43 0.7174237481036343 1105 44-45 1.0143759448600775 1105 46-47 1.1886521953269025 1105 48-49 1.2846745575458627 1105 50-51 1.7221990737427504 1105 52-53 1.3857520589738712 1105 54-55 1.5970257862353705 1105 56-57 1.5661876374773485 1105 58-59 1.7887812455318723 1105 60-61 1.8668585387850527 1105 62-63 2.1609530139361723 1105 64-65 2.4603854716748152 1105 66-67 2.1095855085641055 1105 68-69 2.0589380493758007 1105 70-71 1.9988763630624682 1105 72-73 1.8271293946958025 1105 74-75 1.9081360434173043 1105 76-77 1.9652411521567288 1105 78-79 1.9953972253955392 1105 80-81 2.211529737260122 1105 82-83 1.965160489816423 1105 84-85 1.5949140890222928 1105 86-87 2.131181744032194 1105 88-89 2.3502540898673594 1105 90-91 2.853482836058461 1105 92-93 3.0065322474856444 1105 94-95 2.666322112709736 1105 96-97 2.7315061105661904 1105 98-99 2.898739442786667 1105 100-101 3.2317832341903383 1106 1 -0.16159493282193793 1106 2 -0.11853308487246039 1106 3 0.0692645589733587 1106 4 0.046694221775091194 1106 5 0.0905499115620998 1106 6 -0.06101207722605295 1106 7 -0.14411059082929967 1106 8 0.09963199909333298 1106 9 -0.5838176308885394 1106 10-11 0.08372255245952687 1106 12-13 -0.009435641919147031 1106 14-15 -0.2643669305676255 1106 16-17 -0.42181280795075793 1106 18-19 -0.04739991656630593 1106 20-21 0.0392873636650819 1106 22-23 0.029405840397792815 1106 24-25 0.012169183607277034 1106 26-27 -0.14403374174028727 1106 28-29 -0.23364495332355517 1106 30-31 -0.1753355141502908 1106 32-33 -0.07744110359213607 1106 34-35 -0.19590198844114326 1106 36-37 -0.04566023568033728 1106 38-39 -0.18491581609748664 1106 40-41 -0.045620902382189854 1106 42-43 -0.3899886915098918 1106 44-45 0.018452031816600822 1106 46-47 -0.652606862644106 1106 48-49 -0.0719830511497932 1106 50-51 -0.2878401774649859 1106 52-53 -0.16134939030148843 1106 54-55 -0.1593902765665618 1106 56-57 -0.472535188609605 1106 58-59 -0.4338183921492913 1106 60-61 -0.5011970167705044 1106 62-63 -0.17607899089474444 1106 64-65 -0.06331621914644003 1106 66-67 -0.5363987909528056 1106 68-69 -0.5444737863729934 1106 70-71 -1.070492598290187 1106 72-73 -0.3636918613428435 1106 74-75 -0.07389898073728673 1106 76-77 -0.4345927850413389 1106 78-79 -0.6151100209812732 1106 80-81 -0.9159612047688626 1106 82-83 -0.7818956696038661 1106 84-85 -0.7697447274028271 1106 86-87 -0.9289782801223971 1106 88-89 -0.8221492917751512 1106 90-91 -0.9333529127338096 1106 92-93 -0.4680299505819896 1106 94-95 -0.5502388776284235 1106 96-97 -0.30993410682511424 1106 98-99 -0.41451538813121047 1106 100-101 -0.12291181411884011 1107 1 -0.08384487342540581 1107 2 0.02466007264714065 1107 3 0.03580055327128662 1107 4 -0.08053314814650747 1107 5 -0.1262474274732952 1107 6 0.11492044831445725 1107 7 -0.2399884715610625 1107 8 0.0548707731489273 1107 9 0.15518213152533633 1107 10-11 0.1772661495134571 1107 12-13 0.39330847782425593 1107 14-15 0.22918260613943175 1107 16-17 0.5459823928095133 1107 18-19 0.189966441238397 1107 20-21 0.2932372329927091 1107 22-23 0.30420983184469463 1107 24-25 0.3016144200054711 1107 26-27 0.33232049917204165 1107 28-29 0.2687309079261482 1107 30-31 -0.09386723189798118 1107 32-33 0.09226666547414197 1107 34-35 0.334063561570737 1107 36-37 0.5274628339324039 1107 38-39 0.31308846045276084 1107 40-41 0.6617858450637613 1107 42-43 0.6155054876300383 1107 44-45 0.14275709240106238 1107 46-47 0.23241927182964162 1107 48-49 0.41006731710870525 1107 50-51 -0.1948489681516108 1107 52-53 0.31485041962960736 1107 54-55 -0.012918430522368851 1107 56-57 0.07675799266861105 1107 58-59 0.07114121820946906 1107 60-61 0.07571555153550946 1107 62-63 0.17156890646805323 1107 64-65 0.009895945263156847 1107 66-67 0.4631260367972523 1107 68-69 0.2152791240570373 1107 70-71 0.43575591679653414 1107 72-73 0.758914458448082 1107 74-75 0.8114716536176658 1107 76-77 0.5219408333953446 1107 78-79 0.5453972253955399 1107 80-81 0.5398596552929078 1107 82-83 0.6641188231497601 1107 84-85 0.893269052592423 1107 86-87 0.95229399357682 1107 88-89 1.040122031579564 1107 90-91 0.618191397078494 1107 92-93 1.162583021620435 1107 94-95 1.2539188795585545 1107 96-97 0.8499316570142774 1107 98-99 0.45988470690324235 1107 100-101 0.13881306114844705 1108 1 -0.015285355651759147 1108 2 -0.10157506903501812 1108 3 0.17530818664405956 1108 4 0.16435446381901642 1108 5 -0.07058859135138817 1108 6 -0.07453366694665675 1108 7 0.1348749164170755 1108 8 0.08887449124931379 1108 9 -0.12524150657956312 1108 10-11 0.003732264098552207 1108 12-13 0.18461037630884647 1108 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30.50531952721635 20.36123714566117 22-23 19.77292445986886 29.079608004892826 30.78658597294722 20.360881562291095 24-25 20.764174242680173 29.176527486126698 30.65853975117841 19.400758520014723 26-27 20.230278656375955 28.576990980515564 32.159538020336065 19.033192342772413 28-29 18.99561921288065 30.32237188331176 31.14590296840997 19.536105935397615 30-31 20.234511818933406 28.848687427215104 30.91484500706736 20.00195574678413 32-33 19.837285049852788 28.956220575476127 31.542200634360736 19.664293740310352 34-35 20.345176473516972 29.070431150523046 31.188931307890233 19.395461068069753 36-37 19.775283296698607 28.526359219502478 31.927697987579762 19.770659496219153 38-39 19.389337135826526 29.068981825552324 31.822885366920445 19.718795671700704 40-41 19.821745105813285 29.324761357837897 31.304482330079136 19.54901120626968 42-43 19.664646342525838 29.074177128318734 30.54168274214109 20.719493787014333 44-45 20.015647365285965 28.312129375261158 30.67772650894834 20.99449675050454 46-47 20.886078199894747 28.905905528610237 30.45144864664969 19.756567624845324 48-49 20.119973829063962 28.44400273088028 30.65786479297936 20.778158647076395 50-51 18.976197079928596 29.02967719912099 31.31022736162375 20.683898359326662 52-53 19.707141536404627 29.542399761047978 29.889093546873003 20.8613651556744 54-55 19.45182293055689 28.420861736059994 30.03346099003282 22.093854343350294 56-57 20.596455544967075 28.583213620265408 29.158191929679834 21.662138905087687 58-59 20.876408291021615 28.646528957219964 29.347406610409127 21.129656141349294 60-61 20.070725532308305 29.129034093333523 30.0873312756909 20.71290909866727 62-63 19.27931292008962 29.153063764714247 30.072548810412886 21.495074504783243 64-65 19.188515378268413 30.011985375707738 28.967799527697952 21.83169971832589 66-67 19.857612520425526 29.084705258984123 28.342540748814475 22.71514147177588 68-69 19.71159745278204 28.611922701430988 29.040311982629696 22.63616786315728 70-71 20.38008306427525 28.418934103289857 28.752649096107074 22.448333736327818 72-73 19.922269475301178 27.580468516648416 30.149558365454364 22.34770364259604 74-75 19.433166701344614 29.115783445992204 28.802614895157003 22.648434957506176 76-77 19.45560176603897 27.95386383284534 29.23943631906817 23.351098082047525 78-79 19.685516917560683 28.10392419111579 29.36793915938647 22.842619731937052 80-81 20.07771721249522 27.60312647275896 29.047741843694148 23.27141447105167 82-83 19.47237923809456 27.472955522907323 29.91380629004201 23.140858948956105 84-85 19.617822162473594 27.644765948971827 29.37018543413781 23.367226454416777 86-87 19.35132828336712 28.09594935808528 29.07002382730538 23.48269853124222 88-89 19.387264845935384 27.698533022997584 28.544304698454724 24.3698974326123 90-91 19.644594308600414 26.992621205896633 28.968124818820286 24.39465966668267 92-93 19.963969283191474 27.17224674460873 29.035137951762234 23.82864602043756 94-95 19.627517864701343 27.037856449255 29.077758135597442 24.256867550446216 96-97 18.881657467801006 27.111900065278448 29.601196712243 24.405245754677548 98-99 19.492713403352973 27.372947086784507 28.36329092306379 24.771048586798734 100-101 19.076511613846282 26.776041796331796 29.03497135215686 25.112475237665066 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 645.0 1 382.0 2 144.5 3 186.0 4 147.0 5 121.0 6 192.0 7 331.5 8 452.0 9 484.5 10 399.0 11 285.5 12 253.0 13 247.5 14 249.0 15 265.0 16 314.0 17 353.5 18 411.5 19 486.0 20 558.5 21 679.5 22 794.5 23 956.0 24 1197.5 25 1456.0 26 1972.5 27 2610.0 28 3186.5 29 3788.0 30 4380.5 31 5123.5 32 6021.5 33 6822.5 34 7759.5 35 8662.5 36 9276.5 37 9791.5 38 9911.5 39 9957.5 40 10058.0 41 9971.5 42 9952.0 43 10157.0 44 10299.5 45 10285.0 46 10715.5 47 12953.0 48 16173.0 49 15798.5 50 12703.0 51 10107.0 52 7888.0 53 6439.0 54 5516.0 55 4778.5 56 4015.0 57 3205.5 58 2509.0 59 1922.0 60 1377.0 61 962.5 62 711.5 63 511.0 64 360.0 65 250.5 66 184.0 67 141.0 68 101.5 69 55.5 70 41.5 71 34.5 72 30.5 73 27.5 74 20.5 75 14.5 76 9.0 77 7.5 78 5.0 79 2.0 80 0.0 81 0.5 82 0.5 83 0.5 84 0.5 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.05440425562177308 3 0.0 4 0.0 5 0.05155958866115749 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.008000625826731335 26-27 0.0012445417952693188 28-29 0.0 30-31 0.0019557085354232153 32-33 0.0 34-35 0.013689959747962508 36-37 0.02631316938569417 38-39 0.003200250330692534 40-41 5.333750551154224E-4 42-43 8.889584251923706E-4 44-45 0.010845292787346921 46-47 0.0 48-49 0.0 50-51 7.111667401538966E-4 52-53 0.0 54-55 0.0017779168503847412 56-57 0.0 58-59 0.028624461291194333 60-61 0.0 62-63 0.013512168062924034 64-65 0.01849033524400131 66-67 0.008356209196808283 68-69 0.020801627149501473 70-71 0.0 72-73 0.0 74-75 0.024713044220347903 76-77 0.05227075540131139 78-79 0.03555833700769482 80-81 0.028624461291194333 82-83 0.04160325429900295 84-85 0.01777916850384741 86-87 0.013512168062924034 88-89 0.08605117555862146 90-91 0.030046794771502128 92-93 0.02809108623607891 94-95 0.030935753196694494 96-97 0.044270129574580055 98-99 0.058315672692619516 100-101 0.019557085354232152 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 281228.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.33588014643563 #Duplication Level Percentage of deduplicated Percentage of total 1 80.296570725281 52.46247121076751 2 12.634002283909968 16.509073179826714 3 3.765030611243821 7.3797476629166265 4 1.487517435313239 3.887530434774363 5 0.6353788905664772 2.0756519520813295 6 0.37148119141530644 1.4562630359379345 7 0.20157385455582952 0.9219003641340308 8 0.12844923239789371 0.6713874922280352 9 0.087781694116541 0.5161764821264456 >10 0.3443622701593617 3.7211870547002457 >50 0.022838364360256342 1.002237247571135 >100 0.014138035080158684 2.1263346071298272 >500 0.00652524696007324 2.9132738142797967 >1k 0.004350164640048826 4.356765461525994 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2751 0.9782098510816847 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1799 0.6396944827684299 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1592 0.5660887251625015 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1511 0.5372864721862688 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1203 0.42776679420256875 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1156 0.4110543758089522 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1143 0.4064317919979518 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1108 0.39398637404525866 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 841 0.2990456142347135 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 801 0.2848222794316355 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 771 0.2741547783293271 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 766 0.27237686147894236 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 749 0.26633194418763423 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 747 0.26562077744748036 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 667 0.23717410784132448 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 660 0.23468502425078586 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 608 0.21619468900678454 TruSeq Adapter, Index 1 (95% over 21bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 543 0.1930817699517829 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 527 0.18739243603055172 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 520 0.1849033524400131 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 448 0.1593013497944728 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 446 0.1585901830543189 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.1546787659834725 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 423 0.15041176554254912 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 334 0.11876484560570072 RNA PCR Primer, Index 1 (95% over 23bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 316 0.11236434494431564 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 310 0.11023084472385396 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 292 0.10383034406246888 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 286 0.1016968438420072 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 8.889584251923706E-4 0.0 22-23 0.0 0.0 0.0 0.004089208755884905 0.0 24-25 0.0 0.0 0.0 0.009600750992077602 0.0 26-27 0.0 0.0 0.0 0.023468502425078586 0.0 28-29 0.0 0.0 0.0 0.053159713826503766 0.0 30-31 0.0 0.0 0.0 0.1264098880623551 0.0 32-33 0.0 0.0 0.0 0.26064261026640306 0.0 34-35 0.0 0.0 0.0 0.47061459029684105 0.0 36-37 0.0 0.0 0.0 0.7657487874607081 0.0 38-39 0.0 0.0 0.0 1.0966191133173084 0.0 40-41 0.0 0.0 0.0 1.3926422689063678 0.0 42-43 0.0 0.0 0.0 1.7007552590780435 0.0 44-45 0.0 0.0 0.0 2.104164592430341 0.0 46-47 0.0 0.0 0.0 2.556644430853258 0.0 48-49 0.0 0.0 0.0 2.9780107243944416 0.0 50-51 0.0 0.0 0.0 3.349595346124852 0.0 52-53 0.0 0.0 0.0 3.709090133272647 0.0 54-55 0.0 0.0 0.0 4.150546887223179 0.0 56-57 0.0 0.0 0.0 4.705612527913295 0.0 58-59 0.0 0.0 0.0 5.208051829832023 0.0 60-61 0.0 0.0 0.0 5.732537300695521 0.0 62-63 0.0 0.0 0.0 6.233376477448902 0.0 64-65 0.0 0.0 0.0 6.788975493194134 0.0 66-67 0.0 0.0 0.0 7.450538353222296 0.0 68-69 0.0 0.0 0.0 8.176106219864309 0.0 70-71 0.0 0.0 0.0 8.888339710128438 0.0 72-73 0.0 0.0 0.0 9.587772199069793 0.0 74-75 0.0 0.0 0.0 10.321696274908614 0.0 76-77 0.0 0.0 0.0 11.124247941172287 0.0 78-79 0.0 0.0 0.0 12.035963702049582 0.0 80-81 0.0 0.0 0.0 12.97434821568265 0.0 82-83 0.0 0.0 0.0 13.872907391867097 0.0 84-85 0.0 0.0 0.0 14.777511485342853 0.0 86-87 0.0 0.0 0.0 15.691005163070532 0.0 88-89 0.0 0.0 0.0 16.691794558152104 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GACCTGC 40 5.392931E-4 47.47902 6 GTCCTAT 60 0.0039338493 31.686485 1 GTACACT 90 8.935165E-4 26.386616 4 GTGTAGG 100 0.0016445476 23.764864 1 GTATAGC 120 1.6991141E-4 23.764864 1 CTCCGAC 75 3.0150555E-5 22.180538 94-95 CACTCTA 65 2.9265124E-4 21.936796 76-77 GTACTGA 110 0.0028616083 21.60442 1 TAGGACG 155 3.895517E-5 21.449766 4 GTATAGT 135 3.77014E-4 21.124323 1 TACTGAG 115 0.0037029337 20.665098 2 TACACTG 185 6.5280256E-6 20.53877 5 GAGTACT 1555 0.0 20.457197 12-13 AGTACTT 1405 0.0 20.444702 12-13 GTACTTT 1490 0.0 20.393671 14-15 GTTGATC 95 8.194502E-6 20.012518 94-95 GTCCTAC 200 1.2722141E-5 19.011892 1 ATAATAC 125 0.00602306 18.998362 3 TATATTG 125 0.00602306 18.998362 5 GTGGAGT 125 0.00602306 18.998362 4 >>END_MODULE