##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938458_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 285586 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.946643042726183 31.0 31.0 34.0 28.0 34.0 2 31.045905611619617 31.0 31.0 34.0 27.0 34.0 3 31.084328363435183 31.0 31.0 34.0 27.0 34.0 4 34.68365746220053 37.0 35.0 37.0 32.0 37.0 5 34.730533009321185 37.0 35.0 37.0 32.0 37.0 6 34.77325569180562 37.0 35.0 37.0 32.0 37.0 7 34.729167396160875 37.0 35.0 37.0 32.0 37.0 8 34.711733068147595 37.0 35.0 37.0 32.0 37.0 9 36.192768553080334 39.0 35.0 39.0 32.0 39.0 10-11 36.06631102364962 39.0 35.0 39.0 31.5 39.0 12-13 35.97452956377414 39.0 35.0 39.0 30.5 39.0 14-15 36.92137219611605 39.0 36.0 41.0 31.0 41.0 16-17 36.81888292843487 39.0 36.0 41.0 31.0 41.0 18-19 36.858126798932716 39.0 36.0 41.0 31.0 41.0 20-21 36.96570735260132 39.0 36.0 41.0 31.0 41.0 22-23 36.87907845622685 39.0 36.0 41.0 31.0 41.0 24-25 36.7746633238324 39.0 36.0 41.0 30.5 41.0 26-27 36.58596534844145 39.0 36.0 41.0 30.0 41.0 28-29 36.41634744000056 39.0 36.0 41.0 30.0 41.0 30-31 36.226054498469814 39.0 35.0 40.0 30.0 41.0 32-33 35.90851967533422 38.5 35.0 40.0 29.0 41.0 34-35 35.66545453908805 38.0 35.0 40.0 28.0 41.0 36-37 35.377522007381316 38.0 35.0 40.0 27.0 41.0 38-39 35.138509940963495 38.0 34.5 40.0 26.0 41.0 40-41 35.05816986827085 38.0 34.0 40.0 25.5 41.0 42-43 34.84571722703494 38.0 34.0 40.0 24.5 41.0 44-45 34.533748152920666 38.0 34.0 40.0 24.0 41.0 46-47 34.10540607732872 38.0 33.0 40.0 22.0 41.0 48-49 33.70992100453104 38.0 33.0 40.0 18.5 41.0 50-51 32.967431876912734 37.5 32.0 39.5 15.0 40.5 52-53 33.06248205444244 37.5 33.0 39.5 14.5 40.5 54-55 33.250968184714935 38.0 33.0 40.0 11.0 41.0 56-57 32.96126910983031 38.0 32.5 40.0 8.5 41.0 58-59 32.693458362804904 38.0 32.0 40.0 7.0 41.0 60-61 32.27629855805256 37.0 31.0 40.0 2.0 41.0 62-63 31.839470422219577 37.0 31.0 40.0 2.0 41.0 64-65 31.427163796544647 36.0 30.5 39.0 2.0 41.0 66-67 30.90092301443348 36.0 29.5 39.0 2.0 40.0 68-69 30.372950704866486 35.0 29.0 38.5 2.0 40.0 70-71 29.762955817161902 35.0 28.0 38.0 2.0 40.0 72-73 29.25494421995476 34.0 28.0 37.5 2.0 39.5 74-75 28.74604847576562 34.0 26.5 37.0 2.0 39.0 76-77 28.073170603601017 34.0 26.0 36.0 2.0 39.0 78-79 27.33379962603209 33.0 25.5 36.0 2.0 38.0 80-81 26.689814976924637 33.0 23.5 35.0 2.0 37.0 82-83 26.059973177956902 32.0 21.5 35.0 2.0 37.0 84-85 25.43655151162872 32.0 19.5 35.0 2.0 36.0 86-87 24.836021373596743 31.5 17.0 34.5 2.0 36.0 88-89 24.094638742795517 31.0 8.5 34.0 2.0 35.0 90-91 23.311979578830893 31.0 2.0 34.0 2.0 35.0 92-93 22.501316591149426 30.0 2.0 34.0 2.0 35.0 94-95 21.609063469497805 29.0 2.0 34.0 2.0 35.0 96-97 20.447348609525676 29.0 2.0 34.0 2.0 35.0 98-99 19.071414565139747 26.5 2.0 34.0 2.0 35.0 100-101 15.687577472285057 12.5 2.0 30.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 1.0854721877069657 1101 2 0.9137844195213773 1101 3 0.8174665482582597 1101 4 0.6950110821052249 1101 5 0.46097425021122973 1101 6 0.6272270935357156 1101 7 0.49316365935928985 1101 8 0.6747010291306665 1101 9 0.8710837483505927 1101 10-11 0.6409542046863379 1101 12-13 0.5188339423521455 1101 14-15 0.5707282661533526 1101 16-17 0.6603477079083646 1101 18-19 0.6371625983667215 1101 20-21 0.6166204101912953 1101 22-23 0.6750754450810135 1101 24-25 0.8454053088760176 1101 26-27 0.6633610057095893 1101 28-29 0.40137996572400425 1101 30-31 0.40109721583315405 1101 32-33 0.4844484347801341 1101 34-35 0.7088763080246068 1101 36-37 0.6035376054040924 1101 38-39 0.36219030379833583 1101 40-41 0.13323685127190288 1101 42-43 0.42073721756383264 1101 44-45 0.7424678274143375 1101 46-47 -0.03626629647661872 1101 48-49 -0.26118309965026043 1101 50-51 -0.11457145117608292 1101 52-53 -0.24505884142935486 1101 54-55 -0.07412112410708005 1101 56-57 -0.0347915362993092 1101 58-59 -0.12830583557812503 1101 60-61 0.007420969299573699 1101 62-63 0.3619328809820779 1101 64-65 0.40939935391571325 1101 66-67 0.5683852832085456 1101 68-69 0.7418081627507078 1101 70-71 0.7531329240233262 1101 72-73 0.4675163735447221 1101 74-75 0.06479418840588735 1101 76-77 0.43045998408551966 1101 78-79 0.21079460360162372 1101 80-81 0.7161585741266272 1101 82-83 0.5825798859955995 1101 84-85 0.3666236286304425 1101 86-87 0.9102445179073868 1101 88-89 0.5674677600665312 1101 90-91 0.1874360676463489 1101 92-93 0.2525034118853746 1101 94-95 0.2348472509559656 1101 96-97 -0.4097335773818358 1101 98-99 -0.10206140280510212 1101 100-101 0.08593261829215315 1102 1 0.2608961934098808 1102 2 0.407338938783564 1102 3 0.6364138854133365 1102 4 0.27955974994019783 1102 5 0.4577069558280087 1102 6 0.127360755578664 1102 7 0.10350910148362402 1102 8 -0.20625064461513887 1102 9 0.026532704301523324 1102 10-11 0.1467833548927686 1102 12-13 0.26842553055424645 1102 14-15 0.2766123663107791 1102 16-17 0.4531121359833321 1102 18-19 0.4905501885886068 1102 20-21 0.49681466569371224 1102 22-23 0.6344050556789327 1102 24-25 0.6566373758852606 1102 26-27 0.5470898796811312 1102 28-29 0.3842489472192341 1102 30-31 -0.05734874014748215 1102 32-33 -0.056622940750806094 1102 34-35 0.11268419111487304 1102 36-37 0.1599578388671219 1102 38-39 0.16269970469535622 1102 40-41 -0.19838610295627745 1102 42-43 -0.30209225937203854 1102 44-45 -0.6174320745668354 1102 46-47 -0.5941865150883174 1102 48-49 -0.5741750502250085 1102 50-51 -0.7807802015844914 1102 52-53 -0.8436568751121882 1102 54-55 -1.0707350190190112 1102 56-57 -0.9504656385359205 1102 58-59 -0.9573669339830886 1102 60-61 -1.008299172085163 1102 62-63 -0.8816810436325291 1102 64-65 -0.8917486545198443 1102 66-67 -1.282997376369078 1102 68-69 -1.1273292715321226 1102 70-71 -1.105237290132969 1102 72-73 -0.7284618840962871 1102 74-75 -0.41024071223866443 1102 76-77 -1.2918942500309747 1102 78-79 -1.4080977836024644 1102 80-81 -0.7521709473632576 1102 82-83 -0.5717997736117297 1102 84-85 -0.9948505151899134 1102 86-87 -0.7959440346812023 1102 88-89 -0.40713645177295277 1102 90-91 -0.6291721837571025 1102 92-93 -0.8998638914086534 1102 94-95 -0.6514954585221595 1102 96-97 -0.40527075028111526 1102 98-99 0.47408883333116947 1102 100-101 0.6976666605293591 1103 1 0.8930210969926122 1103 2 0.9551353055149505 1103 3 0.4425021371355484 1103 4 0.5797166773546181 1103 5 0.5197331813247246 1103 6 0.7464261065375197 1103 7 0.43902789287655963 1103 8 0.6821713039445854 1103 9 0.4890829077752059 1103 10-11 0.6293285047717561 1103 12-13 0.6437746275566667 1103 14-15 1.2001700969807843 1103 16-17 0.8405765613364196 1103 18-19 0.7731392249478901 1103 20-21 0.7164997469501984 1103 22-23 1.0742606292619286 1103 24-25 1.0344534250381159 1103 26-27 1.3749351864875266 1103 28-29 1.166432366109042 1103 30-31 1.3865430597301724 1103 32-33 1.3483362315235823 1103 34-35 1.2394895213273927 1103 36-37 1.0589125426998791 1103 38-39 1.478569321952051 1103 40-41 0.9335459660917849 1103 42-43 1.196737434486515 1103 44-45 1.4976502458310677 1103 46-47 1.5872508336363111 1103 48-49 1.3241014080030453 1103 50-51 1.480271212092127 1103 52-53 1.4727247510813868 1103 54-55 1.2109762877341836 1103 56-57 1.2302269699168775 1103 58-59 1.6006696114859622 1103 60-61 1.0572047245023342 1103 62-63 1.0206050430233198 1103 64-65 1.068268102136269 1103 66-67 1.2256203324906672 1103 68-69 1.2164282091179324 1103 70-71 1.5358577440452876 1103 72-73 1.7407643839570106 1103 74-75 1.7485751507291276 1103 76-77 1.2003524717819367 1103 78-79 1.0752586012154737 1103 80-81 1.3129738780217473 1103 82-83 1.2125979176934258 1103 84-85 0.5922774471475059 1103 86-87 1.3052674981538637 1103 88-89 1.7553499168202826 1103 90-91 1.946292613966122 1103 92-93 1.23500724192645 1103 94-95 0.9201059990409455 1103 96-97 0.8394495731489471 1103 98-99 1.2488381484361 1103 100-101 0.19968144894332873 1104 1 0.47909853724387474 1104 2 0.38801305984581447 1104 3 0.46199715602940117 1104 4 0.5542345160943682 1104 5 0.6665285879105767 1104 6 0.7271887310651692 1104 7 0.535828206956154 1104 8 0.33043895865332473 1104 9 0.5842146739822027 1104 10-11 0.6066129431090914 1104 12-13 0.5960247134378065 1104 14-15 0.5907452552474552 1104 16-17 0.5145840207269146 1104 18-19 0.769108945711487 1104 20-21 0.8363346097857516 1104 22-23 0.8428907023835848 1104 24-25 0.8842473103009141 1104 26-27 0.435542734212909 1104 28-29 0.4734329059333504 1104 30-31 0.44226151681413484 1104 32-33 0.6428717372408101 1104 34-35 0.5831981143489386 1104 36-37 0.47333812055726554 1104 38-39 0.6958547417881462 1104 40-41 0.498447570842238 1104 42-43 0.6127824852790766 1104 44-45 0.6597693016292752 1104 46-47 0.6540663609465156 1104 48-49 0.5598696761484945 1104 50-51 0.5320759429161015 1104 52-53 0.7366436302098194 1104 54-55 0.9635220501133404 1104 56-57 0.7722657881923922 1104 58-59 0.2989630305368607 1104 60-61 0.6991785184857449 1104 62-63 0.9183942170921178 1104 64-65 1.0869901907656079 1104 66-67 0.865440963594363 1104 68-69 0.5211897049220262 1104 70-71 0.7428435613883693 1104 72-73 1.0130444055499872 1104 74-75 0.6074724368850859 1104 76-77 0.803041778353073 1104 78-79 0.860058866220136 1104 80-81 1.2242831973523671 1104 82-83 1.123367412712323 1104 84-85 0.6165756755422649 1104 86-87 1.2640629538146584 1104 88-89 1.0056014258746124 1104 90-91 0.9858960884716907 1104 92-93 1.1110623530811843 1104 94-95 0.8200667390910006 1104 96-97 0.5910199711466895 1104 98-99 0.8389151960878642 1104 100-101 0.7140358681414458 1105 1 0.5422290936318497 1105 2 0.4973178246045791 1105 3 0.5508743910708525 1105 4 0.523942836941437 1105 5 0.3606900103458983 1105 6 0.5640182186288527 1105 7 0.46675418837659066 1105 8 0.3589326156306498 1105 9 0.6463962742219422 1105 10-11 0.5634229520992093 1105 12-13 0.24388706485125056 1105 14-15 0.3179203626293372 1105 16-17 0.5008491291076922 1105 18-19 0.4241758720924693 1105 20-21 0.44660081695786147 1105 22-23 0.23969172126282245 1105 24-25 0.5004195208692863 1105 26-27 0.28281073940718926 1105 28-29 0.19236707834370037 1105 30-31 0.22711068273162738 1105 32-33 0.5401097753988395 1105 34-35 0.4623810131602468 1105 36-37 0.26456776794513104 1105 38-39 0.05959213066038416 1105 40-41 0.005839439786399225 1105 42-43 0.4104300729326624 1105 44-45 0.2886775026081949 1105 46-47 0.028005028412302124 1105 48-49 -0.2860747849442973 1105 50-51 -0.05688869598110813 1105 52-53 0.11783681770357646 1105 54-55 0.0072114917275669654 1105 56-57 0.601616002956149 1105 58-59 0.4228642392073212 1105 60-61 0.5691465536470659 1105 62-63 0.7036554297581894 1105 64-65 0.9980380381335046 1105 66-67 0.9254374674401369 1105 68-69 0.888722918387213 1105 70-71 0.7284344114532999 1105 72-73 0.6014471625173208 1105 74-75 0.41658241590816303 1105 76-77 0.6599491802110258 1105 78-79 0.36416684744079575 1105 80-81 0.2515781728792881 1105 82-83 0.7359935239899151 1105 84-85 0.8523288470536023 1105 86-87 1.2928437948894782 1105 88-89 0.9052992582589887 1105 90-91 0.9843977366586891 1105 92-93 1.3390240951648913 1105 94-95 1.3514821821036875 1105 96-97 1.350359697447761 1105 98-99 0.9750904033598644 1105 100-101 0.13866877194725724 1106 1 0.6501038796430976 1106 2 0.2898343323081747 1106 3 0.019962187598121517 1106 4 0.4494992076922344 1106 5 0.5001128521165015 1106 6 0.041127536310433754 1106 7 0.30629930943308636 1106 8 0.25766091105740685 1106 9 0.5372986953518009 1106 10-11 0.11709133067586919 1106 12-13 0.1284676469006527 1106 14-15 0.5297447695747977 1106 16-17 0.0943071873126371 1106 18-19 -0.29705181193785535 1106 20-21 0.03362698472503922 1106 22-23 0.1025677300669301 1106 24-25 -0.027068862598554233 1106 26-27 -0.0689011705952467 1106 28-29 -0.09283712806373501 1106 30-31 -0.283539843069299 1106 32-33 0.5409290466142949 1106 34-35 0.30116800264422494 1106 36-37 0.3823716319705639 1106 38-39 0.2278484280436075 1106 40-41 0.2786078428189285 1106 42-43 -0.04863082215171488 1106 44-45 0.28716123946317396 1106 46-47 0.2856107999945934 1106 48-49 0.5914869660950757 1106 50-51 0.024365889492109716 1106 52-53 0.2504731591073437 1106 54-55 0.17055216181805122 1106 56-57 -0.04714897625637349 1106 58-59 -0.9452453230328359 1106 60-61 -0.24562478408832078 1106 62-63 0.12070116221111249 1106 64-65 0.286140263616506 1106 66-67 -0.5613074579695017 1106 68-69 -0.6872002902212522 1106 70-71 -0.008224918456214425 1106 72-73 0.32929015591424005 1106 74-75 0.2539998773901857 1106 76-77 0.04806363362262189 1106 78-79 0.5204886865212508 1106 80-81 0.8843123377117657 1106 82-83 1.1310167570542369 1106 84-85 0.7874474579609156 1106 86-87 0.8611367760584656 1106 88-89 0.5292415424360506 1106 90-91 -0.15111216796347193 1106 92-93 -0.3108536255492176 1106 94-95 0.11462720533675252 1106 96-97 0.3448082178684011 1106 98-99 0.49165924659782334 1106 100-101 -0.08678977537482702 1107 1 0.5694392614571413 1107 2 0.7549191166486757 1107 3 0.405554539936805 1107 4 0.5695856990999957 1107 5 0.7320122489527421 1107 6 0.46195375686352236 1107 7 0.46008330852264834 1107 8 0.7187739189099176 1107 9 0.5558829675721242 1107 10-11 0.6262696623021426 1107 12-13 0.7482582466502805 1107 14-15 0.9352927381647618 1107 16-17 0.34806842550541717 1107 18-19 0.7817350322638106 1107 20-21 0.9513406526435588 1107 22-23 1.1439470404117529 1107 24-25 1.1054507686749204 1107 26-27 0.937750701488504 1107 28-29 0.651569381552747 1107 30-31 0.786779947530448 1107 32-33 0.7709222183452553 1107 34-35 1.0829308340997912 1107 36-37 1.1451063537186101 1107 38-39 1.19766178869002 1107 40-41 1.6278112114316485 1107 42-43 1.3144396228238193 1107 44-45 1.693283920696814 1107 46-47 1.6382852053661665 1107 48-49 1.64826971333531 1107 50-51 1.4787302914495442 1107 52-53 1.8580411572864648 1107 54-55 1.453475799009361 1107 56-57 1.3908161995715531 1107 58-59 1.4477044891897677 1107 60-61 1.6698241746006488 1107 62-63 1.8945830331568239 1107 64-65 1.4774285303742154 1107 66-67 0.8011484427929894 1107 68-69 0.9267293786076998 1107 70-71 1.2992861774809654 1107 72-73 1.7586517127595798 1107 74-75 1.1261007081629195 1107 76-77 0.9279430008696927 1107 78-79 1.2445298837444234 1107 80-81 1.0093550143932255 1107 82-83 0.7439108050797252 1107 84-85 1.1369296475591852 1107 86-87 1.6977135371828531 1107 88-89 1.4394611850899715 1107 90-91 1.7945234300790922 1107 92-93 1.347994673073746 1107 94-95 1.0852087462494637 1107 96-97 1.8574518960293211 1107 98-99 1.6819403436730447 1107 100-101 1.5503673886174365 1108 1 0.6065362403858039 1108 2 0.5048873827060518 1108 3 0.4719736818509972 1108 4 0.42151512281159853 1108 5 0.2993834091456833 1108 6 0.432948933304246 1108 7 0.44193149334113 1108 8 0.37373076074794653 1108 9 0.3166090401793866 1108 10-11 0.07620936781115262 1108 12-13 0.5487152154240817 1108 14-15 0.3475166528639235 1108 16-17 0.7428704057034423 1108 18-19 0.7764671977880582 1108 20-21 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2316 70-71 -1.9177739900742523 2316 72-73 -1.9876975041019058 2316 74-75 -1.8290412943316454 2316 76-77 -1.5994972013445086 2316 78-79 -1.5335477567955493 2316 80-81 -1.4353451412410685 2316 82-83 -1.4483858493348798 2316 84-85 -1.250875688158331 2316 86-87 -1.4194536979353067 2316 88-89 -1.5499534754396258 2316 90-91 -1.988455034991496 2316 92-93 -2.138566692092869 2316 94-95 -1.658005708975324 2316 96-97 -1.5762158854645492 2316 98-99 -1.8442305008361508 2316 100-101 -1.6775982173077058 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2542.0 3 507.0 4 383.0 5 382.0 6 411.0 7 486.0 8 577.0 9 706.0 10 903.0 11 1187.0 12 1595.0 13 2024.0 14 2346.0 15 3040.0 16 3559.0 17 4156.0 18 3690.0 19 3156.0 20 3295.0 21 3446.0 22 3752.0 23 4019.0 24 4342.0 25 5048.0 26 5759.0 27 6547.0 28 7368.0 29 8857.0 30 10004.0 31 11975.0 32 14193.0 33 17184.0 34 21186.0 35 26800.0 36 33450.0 37 40001.0 38 25063.0 39 1647.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.31358860570767 21.114872568451702 15.360895265825894 21.210643560014734 2 15.327024877643753 22.851277164488135 38.80961473109653 23.012083226771583 3 20.15722763675091 25.254864317594222 31.543269129712797 23.04463891594207 4 12.421740059629958 19.04760236417718 38.693956773535795 29.836700802657074 5 12.22425440071757 38.70459132189708 35.32662013678664 13.744534140598722 6 28.681950532412515 37.91008377744841 17.984646056923815 15.423319633215252 7 24.852437883792863 34.64776003334816 22.705825069271 17.793977013587973 8 23.973855110233075 34.19992574066679 21.164960803682142 20.661258345417988 9 23.96983146677503 18.37786333079944 23.355391066442937 34.2969141359826 10-11 23.469044591342364 27.50797837868135 28.571003191351473 20.451973838624813 12-13 25.460306450595876 26.01256909851399 28.26488289159328 20.26224155929686 14-15 21.61898973835341 28.366035508554877 28.46586719583299 21.549107557258726 16-17 19.418217101103565 30.68170075783449 29.57393483709273 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19.379338605622063 29.233883678086848 30.338892819366905 21.047884896924188 44-45 20.13187451960872 28.317129155439297 30.609175539132522 20.941820785819463 46-47 20.708378640079705 29.01820499486086 30.551717979292846 19.72169838576659 48-49 20.196531974092238 28.557370561877633 30.679754790291767 20.566342673738365 50-51 19.063310909479302 29.27153407877181 31.05785494416215 20.607300067586735 52-53 19.72478831217039 29.7089598756137 29.67411630399003 20.892135508225884 54-55 19.698866591141094 28.39755633337944 30.149235960210746 21.754341115268712 56-57 20.550595576815663 28.352713144361935 29.61495429212767 21.481736986694727 58-59 21.05569115798247 28.166530675136663 29.594441740177967 21.1833364267029 60-61 20.088109275112107 28.292322481347693 30.78624220595368 20.83332603758652 62-63 19.62310994698397 28.179035906630155 30.68889324658407 21.508960899801803 64-65 19.511494756010013 29.184423861467618 29.6571708717805 21.64691051074186 66-67 20.059564141756354 28.284388136895977 29.137230389968295 22.518817331379378 68-69 19.767928462994448 27.898136458904215 29.849750576501837 22.484184501599497 70-71 20.529542696328157 27.6812936827192 29.54777495048343 22.241388670469213 72-73 20.008405568689255 26.8265475877769 31.047368881884246 22.117677961649594 74-75 19.486691848746414 28.25418840328453 29.7938326670064 22.46528708096266 76-77 19.673331021996223 27.13072012159034 29.95037629268532 23.245572563728118 78-79 19.844486737950874 27.354015908505957 30.150873427052165 22.650623926491008 80-81 20.30781083115928 26.86971442578747 29.779209637035354 23.043265106017895 82-83 19.635778518601523 26.848771571044374 30.679382844016136 22.836067066337964 84-85 19.873095691241538 26.976095136210276 30.14029859631368 23.01051057623451 86-87 19.667007515005498 27.04596549961129 30.060021991721587 23.22700499366162 88-89 19.877264511790173 26.80250234682583 29.506956412088602 23.813276729295392 90-91 20.24236755868717 25.989795971983586 29.859830353217703 23.90800611611154 92-93 20.674564606372638 26.2199339795284 30.051222779689514 23.05427863440945 94-95 20.13054908025423 25.892467769929578 30.045904097041365 23.931079052774827 96-97 19.807442722354267 25.921495340197954 30.35220432019084 23.918857617256943 98-99 20.412934408573904 25.944819494426785 29.415359829082284 24.226886267917028 100-101 20.49462531466276 25.028924431854037 29.972723384263706 24.5037268692195 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 682.0 1 412.5 2 156.5 3 168.0 4 126.5 5 115.5 6 204.5 7 362.5 8 476.0 9 488.0 10 394.5 11 285.5 12 270.5 13 278.5 14 288.0 15 297.5 16 332.5 17 388.0 18 446.5 19 542.0 20 654.0 21 778.0 22 877.5 23 1066.5 24 1319.5 25 1593.0 26 2097.5 27 2692.5 28 3222.0 29 3833.5 30 4570.0 31 5219.0 32 5924.5 33 6855.5 34 7900.0 35 8792.0 36 9427.0 37 9829.0 38 9835.5 39 9920.5 40 10134.5 41 10131.5 42 10135.5 43 10304.0 44 10319.5 45 10262.5 46 10809.0 47 12801.5 48 15758.5 49 15582.0 50 12345.0 51 9699.0 52 7986.5 53 6761.5 54 5714.5 55 4930.0 56 4239.0 57 3418.0 58 2718.5 59 2147.0 60 1640.5 61 1229.5 62 878.5 63 639.5 64 472.0 65 360.0 66 301.0 67 238.5 68 172.5 69 125.0 70 108.5 71 100.0 72 85.5 73 69.0 74 46.0 75 27.0 76 32.0 77 28.5 78 16.5 79 12.5 80 9.0 81 5.0 82 4.0 83 2.0 84 1.0 85 1.0 86 1.0 87 0.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18593348413437633 2 0.052173425868214836 3 0.04902201088288642 4 0.055674998074135285 5 0.062327985265384155 6 0.06547940025071257 7 0.03956776592690118 8 0.035015722059204585 9 0.043069338132821634 10-11 0.04446996701518982 12-13 0.04341949535341368 14-15 0.03694158677246084 16-17 0.03554095789009266 18-19 0.04341949535341368 20-21 0.05234850447851085 22-23 0.04989740393436653 24-25 0.06898097245663304 26-27 0.0549746836329512 28-29 0.046921067559334145 30-31 0.05585007668443131 32-33 0.05252358308880687 34-35 0.050422639765254594 36-37 0.049372168103478456 38-39 0.02836273486795571 40-41 0.027662420426771618 42-43 0.023810691000259114 44-45 0.0040268080368085265 46-47 0.011380109669241488 48-49 0.012080424110425581 50-51 0.009454244955985238 52-53 0.0084037732942091 54-55 0.011029952448649443 56-57 0.007878537463321032 58-59 0.01015455939716933 60-61 0.011029952448649443 62-63 0.00420188664710455 64-65 0.007353301632432962 66-67 0.0040268080368085265 68-69 0.00962932356628126 70-71 0.026436870154699463 72-73 0.021359590456114796 74-75 0.022935297948779004 76-77 0.012955817161905695 78-79 0.00542743691917671 80-81 0.007353301632432962 82-83 0.006302829970656824 84-85 0.005777594139768756 86-87 0.0084037732942091 88-89 0.03291477873565231 90-91 0.038692372875421065 92-93 0.023985769610555138 94-95 0.03484064344890856 96-97 0.039392687316605154 98-99 0.023985769610555138 100-101 0.12605659941313652 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 285586.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.68687684773155 #Duplication Level Percentage of deduplicated Percentage of total 1 81.75680447537908 55.33862755989053 2 11.732192217226654 15.882308995226705 3 3.490097554616562 7.08701410097701 4 1.3677094245215689 3.7030391752429295 5 0.6079116690657871 2.057382113917744 6 0.322373065236901 1.309225557942951 7 0.19739920258853402 0.9352934860815362 8 0.10957159629621771 0.5933247315609158 9 0.07072615278578784 0.43085091541729204 >10 0.3032532253532598 3.389032739749731 >50 0.02123972955695957 0.9705897204783644 >100 0.011914970239269967 1.9860622025973422 >500 0.0051804218431608555 2.391058997088855 >1k 0.0036262952902125986 3.926189703828115 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2618 0.9167116035099759 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1886 0.6603965180365984 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1651 0.5781095711974676 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1522 0.5329392897410937 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1296 0.4538037578872914 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1166 0.40828331921032546 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1058 0.37046633938638446 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 790 0.2766242042677162 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 777 0.2720721604000196 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 774 0.27102168873824345 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 739 0.2587661860175219 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 730 0.25561477103219343 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 683 0.23915738166436729 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 644 0.2255012500612775 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 644 0.2255012500612775 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 531 0.18593348413437633 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 507 0.17752971084016722 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 461 0.1614224786929331 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 454 0.1589713781487888 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 425 0.14881681875161948 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 424 0.14846666153102742 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 404 0.1414635171191865 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 334 0.1169525116777433 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 307 0.10749826672175807 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 299 0.1046970089570217 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 297 0.10399669451583761 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7507861029602293E-4 0.0 16-17 0.0 0.0 0.0 3.5015722059204585E-4 0.0 18-19 0.0 0.0 0.0 8.753930514801146E-4 0.0 20-21 0.0 0.0 0.0 0.0015757074926642063 0.0 22-23 0.0 0.0 0.0 0.0035015722059204583 0.0 24-25 0.0 0.0 0.0 0.007703458853025008 0.0 26-27 0.0 0.0 0.0 0.01925864713256252 0.0 28-29 0.0 0.0 0.0 0.0465709103387421 0.0 30-31 0.0 0.0 0.0 0.10732318811146205 0.0 32-33 0.0 0.0 0.0 0.2475611549585764 0.0 34-35 0.0 0.0 0.0 0.46255768840209255 0.0 36-37 0.0 0.0 0.0 0.7759484008319735 0.0 38-39 0.0 0.0 0.0 1.109122996225305 0.0 40-41 0.0 0.0 0.0 1.4345941327656118 0.0 42-43 0.0 0.0 0.0 1.750435945739637 0.0 44-45 0.0 0.0 0.0 2.136309202832072 0.0 46-47 0.0 0.0 0.0 2.584160287969298 0.0 48-49 0.0 0.0 0.0 2.988416799142815 0.0 50-51 0.0 0.0 0.0 3.392148074485444 0.0 52-53 0.0 0.0 0.0 3.750533989761403 0.0 54-55 0.0 0.0 0.0 4.1962841315750765 0.0 56-57 0.0 0.0 0.0 4.76493945781656 0.0 58-59 0.0 0.0 0.0 5.2959528828443965 0.0 60-61 0.0 0.0 0.0 5.827666622313418 0.0 62-63 0.0 0.0 0.0 6.3402967932601735 0.0 64-65 0.0 0.0 0.0 6.894595673457382 0.0 66-67 0.0 0.0 0.0 7.53608370158201 0.0 68-69 0.0 0.0 0.0 8.262659934310506 0.0 70-71 0.0 0.0 0.0 8.970852912957918 0.0 72-73 0.0 0.0 0.0 9.664514366950762 0.0 74-75 0.0 0.0 0.0 10.415426526510403 0.0 76-77 0.0 0.0 0.0 11.1859474904232 0.0 78-79 0.0 0.0 0.0 12.077097616829956 0.0 80-81 0.0 0.0 0.0 12.974725651817666 0.0 82-83 0.0 0.0 0.0 13.83733796474617 0.0 84-85 0.0 0.0 0.0 14.74371993024868 0.0 86-87 0.0 0.0 0.0 15.658330590435105 0.0 88-89 0.0 0.0 0.0 16.64367300918112 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGCAGAA 70 0.008376353 27.146065 9 CGCCGTA 80 6.165101E-8 26.747696 94-95 CATACTG 95 0.0012172102 25.024889 5 TAGGACG 95 0.0012172102 25.024889 4 GTAAGCG 50 0.0016422431 23.77573 94-95 GAGTACT 1360 0.0 22.006275 12-13 GTACAGG 110 0.0028064817 21.677065 1 GTACTTT 1415 0.0 20.986721 14-15 TAGCCCT 250 9.30595E-9 20.920809 4 GTATAGA 115 0.003631708 20.734585 1 TTACACT 120 0.00472758 19.81137 4 AAGCTTT 145 6.111184E-4 19.664389 6 GTATAGC 195 9.93422E-6 19.564941 1 ACCTAGA 125 0.0058833105 19.075819 1 GTATAAG 125 0.0058833105 19.075819 1 AGTACTT 1385 0.0 19.036545 12-13 TCGCCGT 125 2.1426968E-7 19.020586 94-95 GGACGTG 125 0.0060039894 19.00891 6 GTCGCCG 190 1.0913936E-11 18.770315 94-95 TGGTCGC 220 0.0 18.351223 92-93 >>END_MODULE