FastQCFastQC Report
Thu 26 May 2016
SRR938458_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938458_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285586
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28721.0056515375403556No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18620.6519927447423893No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16670.5837120867269404No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16420.5749581562121392No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12180.4264914946811118No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT11910.41703724972512657No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11220.3928764015042754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11150.3904253009601311No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8590.3007850524885674No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG8070.282576877017781No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7970.27907530481186055No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC7800.2731226320617957No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA7760.2717220031794276No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC7320.25631508547337756No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7180.2514128843850889No Hit
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT7030.2461605260762082No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT6580.23040345114956617TruSeq Adapter, Index 1 (95% over 21bp)
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA5690.19923945851687408No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5210.18243191192845587No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC4990.1747284530754309No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT4800.16807546588418198No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4350.15231839095753993No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC4280.1498672904133956No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG4230.14811650431043538No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT3820.13376005826616152RNA PCR Primer, Index 1 (95% over 23bp)
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3010.10539732339820579No Hit
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC2930.10259606563346943No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGAGTG250.005019509256.9940079
TATTGTC650.00579496429.2738325
GGAACGG350.00839239827.14000324-25
GTTGCCG350.00839239827.14000344-45
CTCCGAC751.0595177E-625.33067194-95
GTGTTAG1050.002160262322.6762311
GTATAGC1703.0672527E-622.4094521
GAGTACT14000.021.71200412-13
TGTAGCC1353.7222684E-421.1644822
GTACTTT14400.020.77906614-15
ACTGAGC2108.5698775E-720.3550058
AGTACTT13600.019.90599812-13
TTGGATC1456.070031E-419.684134
GGACGTG1250.006023946718.9980036
TATCTTA1300.0074857218.2961465
TAGGACG1300.0074857218.2961464
TAGGACC1300.0074857218.2961464
TACTGGC650.007563167318.26731132-33
TATATGC1851.4872292E-418.018412
GTGTAGC1600.001168079617.857531
Produced by FastQC (version 0.11.5)