##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938458_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 285586 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.707832316710203 31.0 31.0 34.0 30.0 34.0 2 31.931785871856462 31.0 31.0 34.0 30.0 34.0 3 31.97601072881724 31.0 31.0 34.0 30.0 34.0 4 35.65425476038742 37.0 35.0 37.0 33.0 37.0 5 35.52887746598223 37.0 35.0 37.0 33.0 37.0 6 35.56926810137752 37.0 35.0 37.0 33.0 37.0 7 35.5099374619204 37.0 35.0 37.0 33.0 37.0 8 35.51888748047873 37.0 35.0 37.0 33.0 37.0 9 37.05818912691799 39.0 37.0 39.0 33.0 39.0 10-11 36.98230480485738 39.0 37.0 39.0 33.0 39.0 12-13 36.91688318054807 39.0 37.0 39.0 33.0 39.0 14-15 37.89587024574034 40.0 37.0 41.0 32.5 41.0 16-17 37.85375683681973 40.0 37.0 41.0 32.5 41.0 18-19 37.99607123598496 40.0 37.0 41.0 33.0 41.0 20-21 38.06615695447256 40.0 37.0 41.0 33.0 41.0 22-23 37.962648729279444 40.0 37.0 41.0 33.0 41.0 24-25 37.84041059435687 40.0 37.0 41.0 32.0 41.0 26-27 37.63858697555202 40.0 37.0 41.0 32.0 41.0 28-29 37.52683955095838 39.0 36.0 41.0 32.0 41.0 30-31 37.304682302353754 39.0 36.0 41.0 31.5 41.0 32-33 37.031006421883426 39.0 35.5 41.0 31.0 41.0 34-35 36.82544137317656 39.0 35.0 40.5 31.0 41.0 36-37 36.77710567044603 39.0 35.0 41.0 30.5 41.0 38-39 36.612713858522476 39.0 35.0 41.0 30.0 41.0 40-41 36.49091867248394 39.0 35.0 41.0 30.0 41.0 42-43 36.23425518057608 39.0 35.0 40.5 30.0 41.0 44-45 35.91866373001478 39.0 35.0 40.0 29.5 41.0 46-47 35.48470863417675 38.5 35.0 40.0 27.5 41.0 48-49 35.102918210276414 38.0 34.5 40.0 26.0 41.0 50-51 34.733101412534225 38.0 34.0 40.0 24.0 41.0 52-53 34.327209667140544 38.0 33.5 40.0 23.5 41.0 54-55 34.067060360101685 38.0 33.0 40.0 23.0 41.0 56-57 33.704805207538186 38.0 33.0 40.0 21.5 41.0 58-59 33.2912222587942 37.5 32.5 40.0 19.0 41.0 60-61 32.88742970593796 37.0 32.0 40.0 16.5 41.0 62-63 32.47386601584111 36.5 31.0 39.5 12.5 41.0 64-65 32.023850959080626 36.0 31.0 39.0 8.0 40.0 66-67 31.48966686042033 36.0 30.5 39.0 6.5 40.0 68-69 30.960838416448986 35.0 29.5 38.5 2.0 40.0 70-71 30.3854968380803 35.0 29.0 38.0 2.0 40.0 72-73 29.967307571099425 34.0 29.0 37.0 2.0 39.0 74-75 29.52689732689978 34.0 28.0 37.0 2.0 39.0 76-77 28.21372721351887 32.5 27.0 35.0 2.0 37.5 78-79 28.902503974284453 34.0 28.0 36.0 2.0 38.0 80-81 28.6256206536735 34.0 28.0 35.5 2.0 37.0 82-83 28.186945438501887 33.5 26.5 35.0 2.0 37.0 84-85 27.709478055646983 33.0 26.0 35.0 2.0 36.0 86-87 27.233512847268422 33.0 26.0 35.0 2.0 36.0 88-89 26.659613916648574 32.5 25.0 35.0 2.0 35.5 90-91 26.16883705783897 32.0 24.5 34.5 2.0 35.0 92-93 25.571654422835852 32.0 22.0 34.0 2.0 35.0 94-95 24.938605183727496 31.5 19.0 34.0 2.0 35.0 96-97 24.069532470079068 31.0 8.5 34.0 2.0 35.0 98-99 23.018145147171083 31.0 2.0 34.0 2.0 35.0 100-101 19.59602536538906 26.5 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.311036441180395 1101 2 0.3576379525398572 1101 3 0.15852006517112116 1101 4 0.18633208013268643 1101 5 -0.011894661161832687 1101 6 0.07813109989881184 1101 7 1.7296683046197359E-4 1101 8 0.22564054648147192 1101 9 0.19488364949396697 1101 10-11 0.18922826828870853 1101 12-13 0.371970418569866 1101 14-15 0.2891000469469418 1101 16-17 0.2457194669085041 1101 18-19 0.42360747136432053 1101 20-21 0.4797451946027351 1101 22-23 0.5813630245400816 1101 24-25 0.58019844451551 1101 26-27 0.86181537270501 1101 28-29 0.7154831684952043 1101 30-31 0.8698155892779198 1101 32-33 0.5212581484606531 1101 34-35 0.21310420533457375 1101 36-37 0.2281985845329686 1101 38-39 0.11171241223593853 1101 40-41 0.3257704501652583 1101 42-43 0.5100862316114316 1101 44-45 0.4251507416141038 1101 46-47 0.6706583822468701 1101 48-49 0.7238522000495635 1101 50-51 0.79940925549014 1101 52-53 0.7173489455291175 1101 54-55 0.9078906671449332 1101 56-57 0.8678226946264402 1101 58-59 0.7013899527798984 1101 60-61 0.46401201102228384 1101 62-63 0.4799520089216216 1101 64-65 0.8235724976244683 1101 66-67 1.210757338741299 1101 68-69 0.6289803448018603 1101 70-71 0.7547228291108112 1101 72-73 0.7280183195786911 1101 74-75 1.1680159575321198 1101 76-77 0.619100296543909 1101 78-79 0.8578559052822321 1101 80-81 0.4950989264603365 1101 82-83 0.480026750015238 1101 84-85 0.1573067293253736 1101 86-87 0.2635757726009409 1101 88-89 0.40518681872186235 1101 90-91 0.06375977130916155 1101 92-93 0.293802637352794 1101 94-95 0.5320632603025253 1101 96-97 0.8538357214625236 1101 98-99 1.11160035941883 1101 100-101 0.9243839032041983 1102 1 0.03937576455342651 1102 2 0.05046772650249309 1102 3 0.3467607756591313 1102 4 0.29228152617778136 1102 5 -0.029404388788293545 1102 6 0.07688358749794588 1102 7 0.12755289376188017 1102 8 0.058637249484412735 1102 9 0.1553345361188505 1102 10-11 0.06910322002185154 1102 12-13 0.23266486713968249 1102 14-15 0.46874183267183867 1102 16-17 0.3746884870172167 1102 18-19 0.3214451165361538 1102 20-21 0.2091389629812639 1102 22-23 0.09929601530254928 1102 24-25 0.09059438118939767 1102 26-27 0.063102983718764 1102 28-29 0.05959609807015198 1102 30-31 0.15011974468232125 1102 32-33 -0.09600801722087482 1102 34-35 -0.12003358538037645 1102 36-37 -0.4489853047354515 1102 38-39 -0.49583355265549756 1102 40-41 -0.9111975006471127 1102 42-43 -1.1764837033397058 1102 44-45 -0.8964549850618724 1102 46-47 -1.2121942627764426 1102 48-49 -1.2808111201155867 1102 50-51 -1.3840938781422025 1102 52-53 -1.408976536396814 1102 54-55 -1.7197120298580657 1102 56-57 -1.5465816182021541 1102 58-59 -1.2896992443567683 1102 60-61 -1.1535066274070367 1102 62-63 -1.3220351001169064 1102 64-65 -1.1849447447790737 1102 66-67 -1.135390224154115 1102 68-69 -1.398368394319533 1102 70-71 -1.1818025518260136 1102 72-73 -0.9533387956973947 1102 74-75 -1.0178262618518445 1102 76-77 -1.0192027895641438 1102 78-79 -1.0484915566240858 1102 80-81 -0.9139663303193011 1102 82-83 -1.252782826127568 1102 84-85 -1.1597025082069266 1102 86-87 -1.5556982939924602 1102 88-89 -1.974101505156117 1102 90-91 -1.8677829856932462 1102 92-93 -1.5262436988220927 1102 94-95 -1.5346712274748207 1102 96-97 -2.0351208235221776 1102 98-99 -2.3443431460910134 1102 100-101 -1.6556573835157344 1103 1 0.29692290927242126 1103 2 0.39989720225853986 1103 3 0.29062597766992937 1103 4 0.3345987323378452 1103 5 0.27895798054968424 1103 6 0.24899838389126927 1103 7 0.18568253176491112 1103 8 0.377106808225804 1103 9 0.2082243943522215 1103 10-11 0.2403203384089565 1103 12-13 0.107202729203145 1103 14-15 0.30442021126587093 1103 16-17 0.31972399516946126 1103 18-19 0.2838889285424244 1103 20-21 0.4487591454426294 1103 22-23 0.45833397045854696 1103 24-25 0.48208838337042437 1103 26-27 0.3720039598604785 1103 28-29 0.3825407479092391 1103 30-31 0.6159414857786842 1103 32-33 0.3615284883513823 1103 34-35 0.47201498232450234 1103 36-37 0.37616696805226724 1103 38-39 0.8257812038410322 1103 40-41 0.6044686429281754 1103 42-43 0.9202327899975344 1103 44-45 0.7367737299951855 1103 46-47 0.6973330931296147 1103 48-49 0.41259526869203 1103 50-51 0.5573642169185788 1103 52-53 0.21531529539827687 1103 54-55 0.5138438389612574 1103 56-57 0.5457196292378086 1103 58-59 0.725807312017416 1103 60-61 0.877812360810104 1103 62-63 0.48962540471060834 1103 64-65 0.8413804939367822 1103 66-67 1.2032870639273803 1103 68-69 1.0889377737257888 1103 70-71 0.8654618575683557 1103 72-73 0.8688079181361523 1103 74-75 0.8486989928227295 1103 76-77 0.8998931489415867 1103 78-79 0.7959109222971037 1103 80-81 0.4722745931586374 1103 82-83 0.26233806117726743 1103 84-85 0.311179477057852 1103 86-87 0.6785624182984158 1103 88-89 0.4931964175504824 1103 90-91 0.25579735316357954 1103 92-93 0.37529099809521327 1103 94-95 0.7623538011178859 1103 96-97 1.1658925552048487 1103 98-99 1.253610148054797 1103 100-101 0.8448546254211458 1104 1 0.43446769015282527 1104 2 0.26262233353399367 1104 3 0.26881216798254215 1104 4 0.14794220782491152 1104 5 0.333271667549738 1104 6 0.14515581629054708 1104 7 0.3093881802902061 1104 8 0.3056536993285164 1104 9 0.299270672018217 1104 10-11 0.44009827431708715 1104 12-13 0.5142609320789333 1104 14-15 0.6885972245651857 1104 16-17 0.554400345957113 1104 18-19 0.4685395149879241 1104 20-21 0.26793640411663233 1104 22-23 0.1777418813384628 1104 24-25 0.37494552622757027 1104 26-27 0.2687932320791546 1104 28-29 0.4434795514622678 1104 30-31 0.28099276908830007 1104 32-33 0.328623742804929 1104 34-35 0.19013467609688917 1104 36-37 0.37756560376900694 1104 38-39 0.4167968096711334 1104 40-41 0.0016075740760328472 1104 42-43 0.1369906542562589 1104 44-45 0.10646136801844364 1104 46-47 0.2767854168278987 1104 48-49 0.11893084311417113 1104 50-51 0.09018308132452546 1104 52-53 -0.12428940598072558 1104 54-55 -0.0978568529953705 1104 56-57 -0.20999146660554402 1104 58-59 -0.3140783433783767 1104 60-61 -0.4538237976037891 1104 62-63 -0.36318658920409774 1104 64-65 -0.5561817085464114 1104 66-67 -0.5911073513447427 1104 68-69 -0.809023162624019 1104 70-71 -1.0111085404293867 1104 72-73 -0.6162917039868283 1104 74-75 -0.8979332395961706 1104 76-77 -1.0241081760817252 1104 78-79 -0.8664412394538914 1104 80-81 -0.36562475414469375 1104 82-83 -0.4079274342342245 1104 84-85 -0.9303857680714565 1104 86-87 -0.4674416549213909 1104 88-89 -0.5343100848787294 1104 90-91 -0.8166863294197242 1104 92-93 -1.1456394627184459 1104 94-95 -0.3683450259911112 1104 96-97 -1.0521351230598626 1104 98-99 -0.8266703152126169 1104 100-101 -0.35323022514566915 1105 1 0.16254180862251033 1105 2 0.09028690250812232 1105 3 0.022333294743102527 1105 4 -0.17255524497700492 1105 5 -0.14287956609325647 1105 6 -0.16170663501301874 1105 7 0.2160158898097322 1105 8 0.0030365781617973653 1105 9 0.11045337104928166 1105 10-11 0.12588141286468613 1105 12-13 -0.012746859350492912 1105 14-15 0.10232961893137826 1105 16-17 -0.0718357942886172 1105 18-19 -0.0651609145395895 1105 20-21 0.1482056079923595 1105 22-23 -0.26263620276901634 1105 24-25 -0.10555092767314278 1105 26-27 0.08410888486109513 1105 28-29 -0.23562343525234297 1105 30-31 0.06023560197213129 1105 32-33 0.0953053929897294 1105 34-35 -0.05785503522066193 1105 36-37 0.2437627868675989 1105 38-39 0.3479350575984128 1105 40-41 -0.08532180360851527 1105 42-43 -0.19348332436725713 1105 44-45 -0.05996805961116536 1105 46-47 -0.5191890113011581 1105 48-49 -0.7073852092282493 1105 50-51 -0.5158003369154116 1105 52-53 -0.40139708753101644 1105 54-55 -0.5891289536846074 1105 56-57 -0.27533209191770425 1105 58-59 -0.2501236934932436 1105 60-61 -0.5411277632415903 1105 62-63 -0.1923994550516639 1105 64-65 -0.27273100432105934 1105 66-67 -0.6720558080423729 1105 68-69 -0.13636308870533043 1105 70-71 -0.5426113872978284 1105 72-73 -0.4105555137960373 1105 74-75 -0.3140879693933982 1105 76-77 -0.2786906353765026 1105 78-79 -0.3631198929350603 1105 80-81 -0.1551687557429382 1105 82-83 -0.276996458307746 1105 84-85 0.01623544859021564 1105 86-87 0.12084872565997173 1105 88-89 -0.5695213524843616 1105 90-91 -0.3416338871062692 1105 92-93 -0.3471927540979287 1105 94-95 -0.24071042405324405 1105 96-97 0.36967588483145875 1105 98-99 0.34994790474543436 1105 100-101 -0.2789993790763461 1106 1 0.06222388247919852 1106 2 0.0515732805956759 1106 3 0.04647122577758367 1106 4 -0.00674453331156144 1106 5 -0.07078369913312343 1106 6 -0.37689372232043183 1106 7 -0.08718782748055531 1106 8 -0.18395290581961632 1106 9 0.28457907415517525 1106 10-11 -0.06911736434245341 1106 12-13 -0.4155715108545266 1106 14-15 -0.08732555348241533 1106 16-17 -0.5514762931881023 1106 18-19 0.12892076802338437 1106 20-21 -0.05519375615535438 1106 22-23 0.16070691290718742 1106 24-25 0.12666966415860514 1106 26-27 -0.013127603277816036 1106 28-29 0.14700067822283103 1106 30-31 -0.075005288221071 1106 32-33 0.30409727364514794 1106 34-35 0.5153046615267058 1106 36-37 -0.5786510062358516 1106 38-39 -0.10473063083396283 1106 40-41 -0.12928120688560085 1106 42-43 -0.2230749115523949 1106 44-45 -0.7683686465517354 1106 46-47 -1.0684064238714726 1106 48-49 -1.061885086948962 1106 50-51 -1.1385687399479352 1106 52-53 -1.2590028591133091 1106 54-55 -1.594007897191581 1106 56-57 -1.069377090694914 1106 58-59 -1.533408115112227 1106 60-61 -1.4301715392259382 1106 62-63 -1.3131380219384354 1106 64-65 -2.01583445259692 1106 66-67 -1.4394439224957836 1106 68-69 -2.4627142748144024 1106 70-71 -1.493345063013166 1106 72-73 -1.2009565898537566 1106 74-75 -0.8856335795670347 1106 76-77 -1.3917745189666206 1106 78-79 -1.303716615850199 1106 80-81 -2.139920528792583 1106 82-83 -1.7864364191783757 1106 84-85 -1.183287662194818 1106 86-87 -0.6974197996518434 1106 88-89 -0.6626248007602236 1106 90-91 -0.9525289714789764 1106 92-93 -1.1990391713148547 1106 94-95 -0.9441342440581337 1106 96-97 -0.5912803391841948 1106 98-99 -1.509149674124707 1106 100-101 -1.3663312450582517 1107 1 0.24301368559743963 1107 2 0.10482239861092424 1107 3 0.18404946636026764 1107 4 0.11366061065110955 1107 5 0.2560741590664932 1107 6 0.18173834778313136 1107 7 0.006234273350216313 1107 8 0.1908394009972696 1107 9 0.21953981160823588 1107 10-11 0.35606187771968933 1107 12-13 0.13011529920578369 1107 14-15 0.29209859582906716 1107 16-17 0.26871262225516546 1107 18-19 0.12376542490286369 1107 20-21 0.2960182615981495 1107 22-23 0.20752038735807332 1107 24-25 0.43517654932988137 1107 26-27 0.38189345055170776 1107 28-29 0.3628422623812426 1107 30-31 0.31781933906923854 1107 32-33 0.2834417598178973 1107 34-35 0.6434826517395251 1107 36-37 0.567195130101922 1107 38-39 0.46211243278273884 1107 40-41 0.4442933919536003 1107 42-43 0.5671549735050831 1107 44-45 0.8304364063673475 1107 46-47 1.3177606411108869 1107 48-49 1.0659628702605488 1107 50-51 0.9142080894591444 1107 52-53 1.3249563988814543 1107 54-55 1.4066578590392211 1107 56-57 1.0995713558738842 1107 58-59 0.7392731185283807 1107 60-61 1.066431396929751 1107 62-63 1.1548373962930398 1107 64-65 1.1076320319825683 1107 66-67 0.924110191536311 1107 68-69 0.859932817481031 1107 70-71 0.3210568944239576 1107 72-73 0.5423972282866814 1107 74-75 0.09754042748884473 1107 76-77 0.6706984524684536 1107 78-79 0.6718495614571864 1107 80-81 0.4369771609763937 1107 82-83 0.22073895353131334 1107 84-85 0.3526233426987737 1107 86-87 0.9705783794764145 1107 88-89 1.1532509247433609 1107 90-91 0.7017443868913418 1107 92-93 0.8831105953859542 1107 94-95 0.9635589390532822 1107 96-97 0.6771332263102892 1107 98-99 0.1901276674025496 1107 100-101 -0.9790204450127256 1108 1 0.03242724233772876 1108 2 0.05871297997675384 1108 3 0.06720508961489813 1108 4 0.05282664263892656 1108 5 0.06674945736555316 1108 6 0.13071016804207858 1108 7 0.09347088162643047 1108 8 0.02274470691247643 1108 9 0.2045613906892214 1108 10-11 0.14050907243915134 1108 12-13 0.08037234414104688 1108 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-0.7021278866521108 2316 82-83 -0.9553154412058227 2316 84-85 -0.8462850096749257 2316 86-87 -0.7884588954885423 2316 88-89 -0.7150063023171391 2316 90-91 -0.8400505952824595 2316 92-93 -0.5552206959371588 2316 94-95 -0.906629919297437 2316 96-97 -1.2498952624917692 2316 98-99 -0.7273309680931064 2316 100-101 -0.45773938714105356 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 0.0 4 2.0 5 16.0 6 37.0 7 68.0 8 197.0 9 329.0 10 511.0 11 826.0 12 1084.0 13 1519.0 14 1919.0 15 2476.0 16 3092.0 17 3865.0 18 3645.0 19 2765.0 20 2694.0 21 2850.0 22 2947.0 23 3166.0 24 3435.0 25 4050.0 26 4659.0 27 5192.0 28 6364.0 29 7678.0 30 9022.0 31 11106.0 32 13448.0 33 16308.0 34 20885.0 35 27459.0 36 36688.0 37 49773.0 38 33664.0 39 1846.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.81656949769771 21.909063851392755 15.846420505278639 21.427946145630898 2 15.448322251105303 23.455553142144463 38.52866782043287 22.56745678631736 3 19.819526440742063 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19.728558122597047 29.201361411273663 30.750456955172872 20.31962351095642 24-25 20.701681282838237 29.128929865136712 30.765217421752855 19.404171430272196 26-27 20.306344353745782 28.51404778663753 32.26438484246798 18.9152230171487 28-29 18.999880946544998 30.346725679830243 31.14718508610366 19.506208287521094 30-31 20.215976973661174 28.797104900100145 31.04196284131575 19.94495528492293 32-33 19.848837127870414 29.052019356691154 31.453747732731998 19.645395782706434 34-35 20.387698427984606 29.091740002871582 31.090640397538845 19.42992117160497 36-37 19.92149475114326 28.506474407008746 31.838220360942067 19.733810480905927 38-39 19.299004305281017 29.112208684446887 31.728818069924486 19.85996894034761 40-41 19.891832078844573 29.088031319322976 31.268898308497434 19.751238293335014 42-43 19.53838450333352 29.031563392907167 30.603430164154855 20.826621939604458 44-45 19.957035709033356 28.328944696168577 30.705986988157687 21.00803260664038 46-47 20.76554873138039 28.930339722535418 30.485212860574396 19.818898685509794 48-49 20.13771683485885 28.313712857072826 30.69688290042229 20.851687407646033 50-51 18.867696595771502 28.945921718851764 31.33066746969389 20.85571421568284 52-53 19.70509758881738 29.414432080003923 29.913581197957882 20.966889133220818 54-55 19.46961685796923 28.288151379969605 30.155889994607577 22.08634176745359 56-57 20.582241426400454 28.613972673730505 29.17107281169245 21.63271308817659 58-59 20.86849495423445 28.65424075409859 29.459602361460295 21.017661930206664 60-61 19.967890582871707 29.17194820474393 30.153088736842843 20.70707247554152 62-63 19.205248156422233 29.170372497251268 30.087959493812722 21.536419852513777 64-65 19.109655235200602 30.130853753335245 29.024532014874676 21.73495899658947 66-67 19.820124235781865 29.102617075066707 28.392498231706035 22.684760457445392 68-69 19.534605223304403 28.72852438236535 29.18618067093742 22.550689723392818 70-71 20.499429243730436 28.43766851316241 28.640059386664614 22.422842856442543 72-73 19.945655599364116 27.537939534851148 30.10739321955558 22.409011646229157 74-75 19.437227315064465 29.02400677904379 28.815663232791522 22.72310267310022 76-77 19.437927629505648 27.930465779134835 29.20994026317817 23.421666328181352 78-79 19.61563241895611 28.055997142717082 29.459952518680886 22.86841791964592 80-81 20.118528634206978 27.394470998126653 29.109897228495896 23.377103139170472 82-83 19.490971992429536 27.52539975173636 29.840834642057402 23.142793613776707 84-85 19.514868404465425 27.769704312747752 29.362997772890896 23.352429509895927 86-87 19.47699510295696 27.849765652766905 29.042599197074033 23.630640047202103 88-89 19.35371481795326 27.815088974949752 28.517504359457398 24.31369184763959 90-91 19.648325404926524 26.937090411851116 28.954063096928493 24.460521086293866 92-93 20.007878565050685 27.164416898646635 29.054397114694396 23.773307421608276 94-95 19.58621921242638 26.929191206851876 29.194883502692708 24.289706078029035 96-97 18.856841721933147 27.08571148445652 29.532259984733145 24.525186808877187 98-99 19.500464834524163 27.402126508523715 28.370137839631216 24.727270817320907 100-101 19.154825516656977 26.691959689900763 28.999670852212645 25.15354394122961 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 611.0 1 372.5 2 153.0 3 163.5 4 126.5 5 121.5 6 211.0 7 368.0 8 473.5 9 487.0 10 376.0 11 256.5 12 254.5 13 264.0 14 273.5 15 292.0 16 322.5 17 365.0 18 431.5 19 486.0 20 543.0 21 681.5 22 817.5 23 996.5 24 1238.5 25 1541.5 26 2033.0 27 2618.0 28 3173.5 29 3712.5 30 4411.5 31 5249.5 32 6088.5 33 6992.5 34 7966.0 35 8746.5 36 9436.5 37 9814.5 38 9855.0 39 9987.0 40 10132.0 41 10276.5 42 10203.5 43 10379.5 44 10625.5 45 10482.0 46 10949.5 47 13222.5 48 16419.0 49 16039.0 50 12884.5 51 10286.0 52 7987.5 53 6559.5 54 5692.5 55 4771.5 56 3986.5 57 3238.0 58 2501.0 59 1926.5 60 1417.0 61 1020.5 62 735.0 63 506.0 64 338.5 65 264.5 66 213.0 67 155.0 68 101.5 69 59.0 70 44.5 71 38.5 72 31.5 73 24.5 74 20.0 75 14.0 76 11.5 77 8.0 78 5.5 79 4.0 80 2.0 81 1.0 82 1.0 83 1.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 3.5015722059204585E-4 2 0.050422639765254594 3 0.0014006288823681834 4 0.0 5 0.09419229233926033 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.7507861029602293E-4 26-27 0.008753930514801146 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01015455939716933 36-37 0.0 38-39 0.0022760219338482978 40-41 0.0040268080368085265 42-43 7.003144411840917E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 1.7507861029602293E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.5015722059204585E-4 82-83 8.753930514801146E-4 84-85 0.004902201088288641 86-87 0.001925864713256252 88-89 0.0 90-91 0.003326493595624435 92-93 3.5015722059204585E-4 94-95 0.0 96-97 0.0 98-99 1.7507861029602293E-4 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 285586.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.98770202307699 #Duplication Level Percentage of deduplicated Percentage of total 1 80.06245741348965 52.030751256231554 2 12.730751476785956 16.546845670064258 3 3.846853849133739 7.499945750214905 4 1.4822940876274373 3.8532354590920264 5 0.6994979103762831 2.272938088264944 6 0.36588731220106874 1.4266905371608558 7 0.21190636096555587 0.9639915210256896 8 0.1251859655438586 0.6508438580988373 9 0.09079859337513033 0.5310712737339752 >10 0.3358687947502174 3.683053929485852 >50 0.024787987161686658 1.1363949955398827 >100 0.01347173215309057 2.186991293419589 >500 0.005927562147359852 2.7735742264024252 >1k 0.0043109542889889834 4.443672141265199 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2872 1.0056515375403556 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1862 0.6519927447423893 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1667 0.5837120867269404 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1642 0.5749581562121392 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1218 0.4264914946811118 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1191 0.41703724972512657 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1122 0.3928764015042754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1115 0.3904253009601311 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 859 0.3007850524885674 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 807 0.282576877017781 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 797 0.27907530481186055 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 780 0.2731226320617957 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 776 0.2717220031794276 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 732 0.25631508547337756 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 718 0.2514128843850889 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 703 0.2461605260762082 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 658 0.23040345114956617 TruSeq Adapter, Index 1 (95% over 21bp) GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 569 0.19923945851687408 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 521 0.18243191192845587 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 499 0.1747284530754309 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 480 0.16807546588418198 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 435 0.15231839095753993 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 428 0.1498672904133956 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 423 0.14811650431043538 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 382 0.13376005826616152 RNA PCR Primer, Index 1 (95% over 23bp) ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 301 0.10539732339820579 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 293 0.10259606563346943 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 1.7507861029602293E-4 0.0 16-17 0.0 0.0 0.0 3.5015722059204585E-4 0.0 18-19 0.0 0.0 0.0 7.003144411840917E-4 0.0 20-21 0.0 0.0 0.0 0.0012255502720721605 0.0 22-23 0.0 0.0 0.0 0.0031514149853284126 0.0 24-25 0.0 0.0 0.0 0.007003144411840917 0.0 26-27 0.0 0.0 0.0 0.01855833269137843 0.0 28-29 0.0 0.0 0.0 0.0465709103387421 0.0 30-31 0.0 0.0 0.0 0.10907397421442228 0.0 32-33 0.0 0.0 0.0 0.24966209828212868 0.0 34-35 0.0 0.0 0.0 0.4674598894903812 0.0 36-37 0.0 0.0 0.0 0.7831266238541105 0.0 38-39 0.0 0.0 0.0 1.1170015336886263 0.0 40-41 0.0 0.0 0.0 1.4456240852142612 0.0 42-43 0.0 0.0 0.0 1.765492706225095 0.0 44-45 0.0 0.0 0.0 2.153466906641082 0.0 46-47 0.0 0.0 0.0 2.600092441506236 0.0 48-49 0.0 0.0 0.0 3.005399424341529 0.0 50-51 0.0 0.0 0.0 3.4080802280223823 0.0 52-53 0.0 0.0 0.0 3.766115986077749 0.0 54-55 0.0 0.0 0.0 4.211515970670831 0.0 56-57 0.0 0.0 0.0 4.781571925794681 0.0 58-59 0.0 0.0 0.0 5.314861372756368 0.0 60-61 0.0 0.0 0.0 5.8465751122253895 0.0 62-63 0.0 0.0 0.0 6.359730519003032 0.0 64-65 0.0 0.0 0.0 6.915780185303201 0.0 66-67 0.0 0.0 0.0 7.559369156751382 0.0 68-69 0.0 0.0 0.0 8.289446961685798 0.0 70-71 0.0 0.0 0.0 9.001841826980314 0.0 72-73 0.0 0.0 0.0 9.700055324840854 0.0 74-75 0.0 0.0 0.0 10.459371257694706 0.0 76-77 0.0 0.0 0.0 11.245124060703255 0.0 78-79 0.0 0.0 0.0 12.148354611220437 0.0 80-81 0.0 0.0 0.0 13.065416371951006 0.0 82-83 0.0 0.0 0.0 13.946587017570888 0.0 84-85 0.0 0.0 0.0 14.874328573529514 0.0 86-87 0.0 0.0 0.0 15.808548038069093 0.0 88-89 0.0 0.0 0.0 16.822428270293365 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGAGTG 25 0.0050195092 56.994007 9 TATTGTC 65 0.005794964 29.273832 5 GGAACGG 35 0.008392398 27.140003 24-25 GTTGCCG 35 0.008392398 27.140003 44-45 CTCCGAC 75 1.0595177E-6 25.330671 94-95 GTGTTAG 105 0.0021602623 22.676231 1 GTATAGC 170 3.0672527E-6 22.409452 1 GAGTACT 1400 0.0 21.712004 12-13 TGTAGCC 135 3.7222684E-4 21.164482 2 GTACTTT 1440 0.0 20.779066 14-15 ACTGAGC 210 8.5698775E-7 20.355005 8 AGTACTT 1360 0.0 19.905998 12-13 TTGGATC 145 6.070031E-4 19.68413 4 GGACGTG 125 0.0060239467 18.998003 6 TATCTTA 130 0.00748572 18.296146 5 TAGGACG 130 0.00748572 18.296146 4 TAGGACC 130 0.00748572 18.296146 4 TACTGGC 65 0.0075631673 18.267311 32-33 TATATGC 185 1.4872292E-4 18.01841 2 GTGTAGC 160 0.0011680796 17.85753 1 >>END_MODULE