##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938457_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 286340 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.722295173569883 31.0 31.0 34.0 30.0 34.0 2 31.9405217573514 31.0 31.0 34.0 30.0 34.0 3 31.99032618565342 33.0 31.0 34.0 30.0 34.0 4 35.67551162953133 37.0 35.0 37.0 33.0 37.0 5 35.55223161276804 37.0 35.0 37.0 33.0 37.0 6 35.599594887197036 37.0 35.0 37.0 33.0 37.0 7 35.53279667528113 37.0 35.0 37.0 33.0 37.0 8 35.55115247607739 37.0 35.0 37.0 33.0 37.0 9 37.07429978347419 39.0 37.0 39.0 33.0 39.0 10-11 37.01334078368373 39.0 37.0 39.0 33.0 39.0 12-13 36.94917580498708 39.0 37.0 39.0 33.0 39.0 14-15 37.930765872738704 40.0 37.0 41.0 33.0 41.0 16-17 37.884052175735135 40.0 37.0 41.0 32.5 41.0 18-19 37.93699448208423 40.0 37.0 41.0 33.0 41.0 20-21 37.969424460431654 40.0 37.0 41.0 33.0 41.0 22-23 37.957751274708386 40.0 37.0 41.0 33.0 41.0 24-25 37.869716071802756 40.0 37.0 41.0 32.0 41.0 26-27 37.67990850038416 40.0 37.0 41.0 32.0 41.0 28-29 37.56972131033038 39.5 36.5 41.0 32.0 41.0 30-31 37.330650625130964 39.0 36.0 41.0 32.0 41.0 32-33 37.066251658867074 39.0 35.5 41.0 31.0 41.0 34-35 36.85087658028917 39.0 35.0 40.5 31.0 41.0 36-37 36.807395054829925 39.0 35.0 41.0 30.5 41.0 38-39 36.63205105818258 39.0 35.0 41.0 30.0 41.0 40-41 36.5008049870783 39.0 35.0 41.0 30.0 41.0 42-43 36.23114130055179 39.0 35.0 40.0 30.0 41.0 44-45 35.90539917580499 39.0 35.0 40.0 29.5 41.0 46-47 35.492573514004334 38.0 35.0 40.0 27.5 41.0 48-49 35.07350527345114 38.0 34.5 40.0 26.0 41.0 50-51 34.696849898721794 38.0 34.0 40.0 24.0 41.0 52-53 34.282016134665085 38.0 33.0 40.0 23.5 41.0 54-55 34.01698505273451 38.0 33.0 40.0 22.5 41.0 56-57 33.643345323741 38.0 33.0 40.0 21.5 41.0 58-59 33.23910560871691 37.5 32.5 40.0 18.5 41.0 60-61 32.79651463295383 37.0 32.0 40.0 14.5 41.0 62-63 32.37919606062722 36.5 31.0 39.0 11.0 41.0 64-65 31.937928686177273 36.0 31.0 39.0 8.0 40.0 66-67 31.398009359502687 36.0 30.5 39.0 4.5 40.0 68-69 30.847702032548717 35.0 29.5 38.0 2.0 40.0 70-71 30.292365719075224 35.0 29.0 38.0 2.0 40.0 72-73 29.75155235035273 34.0 28.0 37.0 2.0 39.0 74-75 29.206027799119926 34.0 28.0 36.5 2.0 39.0 76-77 27.89606935810575 32.5 26.0 35.0 2.0 37.5 78-79 28.52687190053782 34.0 27.5 36.0 2.0 38.0 80-81 28.246154920723615 34.0 27.0 35.0 2.0 37.0 82-83 27.81236816372145 33.0 26.0 35.0 2.0 37.0 84-85 27.277692603198993 33.0 26.0 35.0 2.0 36.0 86-87 26.728656492281903 32.5 25.0 35.0 2.0 36.0 88-89 26.054513864636448 32.0 23.5 35.0 2.0 35.0 90-91 25.567524621079833 32.0 21.5 34.0 2.0 35.0 92-93 24.892830201857933 31.0 19.0 34.0 2.0 35.0 94-95 24.219167772578054 31.0 12.0 34.0 2.0 35.0 96-97 23.328598868478032 31.0 2.0 34.0 2.0 35.0 98-99 22.277111126632676 30.5 2.0 34.0 2.0 35.0 100-101 19.217171893553118 25.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.36529023683696593 1101 2 0.3699609558113224 1101 3 0.3781632220988165 1101 4 0.38332480465170704 1101 5 0.3546487486519396 1101 6 0.38262779459373775 1101 7 0.3960793081801768 1101 8 0.254631139266408 1101 9 0.4163439899718213 1101 10-11 0.4652206968524695 1101 12-13 0.17329958627583153 1101 14-15 0.40944594559449854 1101 16-17 0.4543061515027418 1101 18-19 0.7723450127613987 1101 20-21 0.7822647886160894 1101 22-23 0.5424746119326613 1101 24-25 0.487903215917477 1101 26-27 0.8658875644610404 1101 28-29 0.5854326899837545 1101 30-31 0.39852707529038156 1101 32-33 0.5329034453995973 1101 34-35 0.44206908986777904 1101 36-37 0.31462085063214573 1101 38-39 0.6145029038832703 1101 40-41 0.3069399704776856 1101 42-43 0.46239721091024677 1101 44-45 0.5758985383810824 1101 46-47 0.7943921503960922 1101 48-49 0.9787133302932105 1101 50-51 0.7215861291455852 1101 52-53 0.619804472849637 1101 54-55 0.7554642202282338 1101 56-57 0.9805381271440652 1101 58-59 1.1586847661895234 1101 60-61 1.2852032556026884 1101 62-63 1.760632278914251 1101 64-65 1.3739593455809747 1101 66-67 1.4924337557630203 1101 68-69 1.8634977011336709 1101 70-71 1.5055293621299484 1101 72-73 2.208080987777908 1101 74-75 2.0255808534665327 1101 76-77 1.594543110890097 1101 78-79 1.6820631257734782 1101 80-81 1.9767756146909257 1101 82-83 1.709813361364695 1101 84-85 1.9923225820602717 1101 86-87 2.060145010100623 1101 88-89 2.7429690375804867 1101 90-91 2.745011277045606 1101 92-93 2.6323902354337854 1101 94-95 2.627082113229367 1101 96-97 2.9820387044278576 1101 98-99 3.4992892950388494 1101 100-101 3.936692312684915 1102 1 0.3367059671740513 1102 2 0.3650770606802354 1102 3 0.275571461799192 1102 4 0.2901263027790506 1102 5 0.16473114565568636 1102 6 0.2178337871031033 1102 7 0.0807834280303652 1102 8 0.23238394825516906 1102 9 0.3677447390354942 1102 10-11 0.2915727567775548 1102 12-13 0.3196890993844477 1102 14-15 0.4826294661937496 1102 16-17 0.6321788106787736 1102 18-19 0.43598546219959644 1102 20-21 0.6361524290655183 1102 22-23 0.4459502673633722 1102 24-25 0.1926822421347012 1102 26-27 0.02298868805655019 1102 28-29 -0.11116656095257582 1102 30-31 0.13729112023420242 1102 32-33 0.05475363266551625 1102 34-35 0.3885335093434392 1102 36-37 0.7022388281602403 1102 38-39 0.5693867990143602 1102 40-41 0.2718763000656992 1102 42-43 0.611258634131211 1102 44-45 0.475411646995326 1102 46-47 0.04257192567698809 1102 48-49 0.32720771231568335 1102 50-51 0.5968670280219825 1102 52-53 0.6356996039357767 1102 54-55 0.42090242247542875 1102 56-57 0.4787328836983704 1102 58-59 0.3731342043917749 1102 60-61 0.4348661769509974 1102 62-63 0.9638008182400881 1102 64-65 1.099577323109063 1102 66-67 0.8170255160626461 1102 68-69 0.6197523827816092 1102 70-71 0.9239563284220829 1102 72-73 0.9048000889015064 1102 74-75 1.0019703665751472 1102 76-77 1.0461236352346646 1102 78-79 1.3729545115412698 1102 80-81 1.595831794466207 1102 82-83 1.4986523126755564 1102 84-85 1.1197233311239465 1102 86-87 1.5436955718983754 1102 88-89 1.7730813971310475 1102 90-91 1.9730262583190132 1102 92-93 2.7008396736360325 1102 94-95 2.755501588884801 1102 96-97 3.2198739104203646 1102 98-99 3.6735814298703104 1102 100-101 3.053898305194277 1103 1 0.43966060720734035 1103 2 0.3024054002557719 1103 3 0.2725335924691912 1103 4 0.3572507305776327 1103 5 0.3380561560593449 1103 6 0.219664831630773 1103 7 0.37370893780980907 1103 8 0.290186694821962 1103 9 0.45130695293478595 1103 10-11 0.6732206968524679 1103 12-13 0.7146329196091727 1103 14-15 0.6720385381870884 1103 16-17 0.7636394848360766 1103 18-19 0.5242709386873159 1103 20-21 0.5370055293568257 1103 22-23 0.7412894267474783 1103 24-25 0.6159032159174771 1103 26-27 0.7335171940906733 1103 28-29 0.32980306035412355 1103 30-31 0.6522307789940882 1103 32-33 0.6200886305847817 1103 34-35 0.5063653861640773 1103 36-37 0.8373615913728898 1103 38-39 0.8802066075869774 1103 40-41 1.1024214519591595 1103 42-43 1.2022490627620925 1103 44-45 0.7488615013440523 1103 46-47 0.7975032615072024 1103 48-49 1.1338244414043217 1103 50-51 1.4067713143307614 1103 52-53 1.2962489172940792 1103 54-55 1.2844271831911982 1103 56-57 1.697501090107032 1103 58-59 1.5252773587821196 1103 60-61 1.5577958481952763 1103 62-63 1.4393730196549868 1103 64-65 1.4643297159513402 1103 66-67 1.984878200207465 1103 68-69 2.0531273307632993 1103 70-71 2.3618256584262447 1103 72-73 2.1615624692593904 1103 74-75 2.1635808534665344 1103 76-77 1.7790616294086163 1103 78-79 2.161989051699404 1103 80-81 2.387071910987224 1103 82-83 2.303146694698029 1103 84-85 2.445878137615832 1103 86-87 2.595256121211733 1103 88-89 3.1481542227656725 1103 90-91 3.3424186844530155 1103 92-93 3.534464309507861 1103 94-95 3.1096006317478846 1103 96-97 3.5991498155389685 1103 98-99 4.035067072816624 1103 100-101 4.922396016388618 1104 1 0.38929023683696684 1104 2 0.3634155012658695 1104 3 0.27125413118972475 1104 4 0.22950662283352585 1104 5 0.13610329410648347 1104 6 0.2153550673210134 1104 7 0.418624762725635 1104 8 0.3873584119936808 1104 9 0.35016217179000364 1104 10-11 0.34412978776155967 1104 12-13 0.4749359499121937 1104 14-15 0.605809581958134 1104 16-17 0.5430334242300177 1104 18-19 0.4901631945795728 1104 20-21 0.766264788616084 1104 22-23 0.5337473392053909 1104 24-25 0.6904486704629349 1104 26-27 0.6929784735519533 1104 28-29 0.5221599627110223 1104 30-31 0.4667088934721946 1104 32-33 0.29126708176323746 1104 34-35 0.6109781807768755 1104 36-37 0.6984390324503309 1104 38-39 0.5092301766105436 1104 40-41 0.4754854250231375 1104 42-43 0.8203972109102367 1104 44-45 1.0095349020174496 1104 46-47 1.1191194231233652 1104 48-49 0.8045315121113958 1104 50-51 1.0124952200546673 1104 52-53 1.37725901830418 1104 54-55 1.1005551293191473 1104 56-57 1.2836290362349771 1104 58-59 1.7654120389167929 1104 60-61 1.1361123465117728 1104 62-63 1.4768140970960602 1104 64-65 1.6975957092173353 1104 66-67 1.7595246648539309 1104 68-69 1.8344067920427598 1104 70-71 2.074620271220855 1104 72-73 2.021899169596093 1104 74-75 1.3826717625574467 1104 76-77 1.4541794745264625 1104 78-79 1.7715176712280254 1104 80-81 2.313502887418199 1104 82-83 2.297086088637421 1104 84-85 2.1203225820602754 1104 86-87 2.258690464646076 1104 88-89 2.4102417648532146 1104 90-91 2.646829458863788 1104 92-93 2.605844780888333 1104 94-95 3.2747184768657327 1104 96-97 3.111675068064219 1104 98-99 3.332743840493393 1104 100-101 4.009601403594004 1105 1 0.37567321556037214 1105 2 0.3381027997829591 1105 3 0.31067386039669387 1105 4 0.313594308197807 1105 5 0.17887569900654654 1105 6 0.2407838229625341 1105 7 0.4866324996695397 1105 8 0.46299993359264846 1105 9 0.480542571532105 1105 10-11 0.5370504840865067 1105 12-13 0.5224343380488818 1105 14-15 0.5968218321193177 1105 16-17 0.5097387756162206 1105 18-19 0.5838060056691958 1105 20-21 0.539172590034525 1105 22-23 0.4005880871099734 1105 24-25 0.4971088896763476 1105 26-27 0.5201996211986284 1105 28-29 0.7977305623241833 1105 30-31 0.5563284937300992 1105 32-33 0.38703110497407067 1105 34-35 0.6600690898677826 1105 36-37 0.5530038293555535 1105 38-39 0.6353681521102246 1105 40-41 0.6858335874989621 1105 42-43 0.408553239279037 1105 44-45 0.1798701695867635 1105 46-47 0.24525739862304619 1105 48-49 0.4688126210733543 1105 50-51 0.22761449793990352 1105 52-53 0.4326555366794267 1105 54-55 0.6239464897317788 1105 56-57 0.6412757157965459 1105 58-59 0.6121173903029984 1105 60-61 0.7361252414182857 1105 62-63 1.0259372434532494 1105 64-65 0.8882855867157282 1105 66-67 0.8670295004438664 1105 68-69 1.3362636585804744 1105 70-71 1.0821960287966164 1105 72-73 1.5914285055084072 1105 74-75 1.4848574492112157 1105 76-77 1.4236636782659815 1105 78-79 1.5172687995323422 1105 80-81 1.8269458274568855 1105 82-83 0.9421537868966112 1105 84-85 1.6509608799326152 1105 86-87 1.7568400455616136 1105 88-89 1.5402740021194923 1105 90-91 2.1048694330739757 1105 92-93 1.9540072567103834 1105 94-95 2.0552665103924888 1105 96-97 2.5036415413072923 1105 98-99 2.4360126992941673 1105 100-101 3.2488341566565495 1106 1 0.24506878354977246 1106 2 0.03719971013658352 1106 3 0.17517360272165305 1106 4 -0.059678655555906346 1106 5 -0.10568343127885527 1106 6 0.043527448572973526 1106 7 -0.005747681439196128 1106 8 -0.1259224939515846 1106 9 0.15477997613098893 1106 10-11 0.09432796328844972 1106 12-13 0.09358332330005936 1106 14-15 -0.2525609748207316 1106 16-17 -0.3195208381166381 1106 18-19 0.10889864597938725 1106 20-21 0.29539973671296593 1106 22-23 0.27560956002955095 1106 24-25 -0.050186749480452875 1106 26-27 0.17758998660637104 1106 28-29 0.20009012943011584 1106 30-31 0.02395960124193408 1106 32-33 0.4227927187559999 1106 34-35 0.5528026538816206 1106 36-37 0.42845476066675303 1106 38-39 0.2588558450597418 1106 40-41 0.07284308466453382 1106 42-43 0.3938228164465727 1106 44-45 -0.1770772401656302 1106 46-47 -0.20910957970770738 1106 48-49 -0.12399947247495646 1106 50-51 -0.2528083345222356 1106 52-53 0.1843304244067312 1106 54-55 0.310564566248992 1106 56-57 -0.0927975129943448 1106 58-59 0.40223493919990005 1106 60-61 0.11330014141583433 1106 62-63 0.37025857649209826 1106 64-65 -0.34029670978234705 1106 66-67 -0.28326866638230896 1106 68-69 -0.5841147556137365 1106 70-71 -0.3310104302575958 1106 72-73 0.23717441338344614 1106 74-75 0.19627981540424955 1106 76-77 0.6143493392638 1106 78-79 0.6524991119326486 1106 80-81 0.7515022582895448 1106 82-83 0.9884984824719609 1106 84-85 0.9379004367315567 1106 86-87 1.1011000274016602 1106 88-89 0.795494989137584 1106 90-91 0.3555995123397224 1106 92-93 0.3199058063680411 1106 94-95 1.2218779609802333 1106 96-97 1.760703064289448 1106 98-99 2.0498498486720678 1106 100-101 2.9038134199513514 1107 1 0.1955722881190205 1107 2 0.09962762247798906 1107 3 0.1848555297911254 1107 4 0.05888890721580964 1107 5 0.43385387685707144 1107 6 0.27044830741424875 1107 7 0.2101049492058209 1107 8 0.15578498542025443 1107 9 0.4973183489461803 1107 10-11 0.27931044044220954 1107 12-13 0.3341842016604488 1107 14-15 0.4380997917483427 1107 16-17 0.4267805104771014 1107 18-19 0.412127064043446 1107 20-21 0.20331607066736979 1107 22-23 0.412692560650612 1107 24-25 0.5678262928405573 1107 26-27 0.6612593593328384 1107 28-29 0.1738557669068328 1107 30-31 0.18378348554679036 1107 32-33 0.6945188300149852 1107 34-35 0.9485306283293227 1107 36-37 0.8921336711449683 1107 38-39 0.841246493626862 1107 40-41 0.8558758679135821 1107 42-43 0.9606536211666494 1107 44-45 1.0314113588939051 1107 46-47 0.6947254837294281 1107 48-49 1.2592517918316801 1107 50-51 1.3632527958122438 1107 52-53 1.2197531907983503 1107 54-55 1.5467847330487459 1107 56-57 1.2207560758620062 1107 58-59 1.5291591251638863 1107 60-61 1.580267358166786 1107 62-63 1.7973502276321938 1107 64-65 1.8888311404527691 1107 66-67 1.8676773455066105 1107 68-69 1.6411259062618733 1107 70-71 1.7921319262325177 1107 72-73 2.010209192906114 1107 74-75 1.8177475201331994 1107 76-77 1.7733636237106083 1107 78-79 2.4806144078247634 1107 80-81 2.6856474095627227 1107 82-83 2.430390284441618 1107 84-85 2.417566171803866 1107 86-87 2.6088244972801107 1107 88-89 2.890212627324079 1107 90-91 3.1430240975584276 1107 92-93 3.3632748508183994 1107 94-95 3.3968513439985983 1107 96-97 3.534192550581704 1107 98-99 3.522994423243979 1107 100-101 4.064846158838762 1108 1 0.10851853265690536 1108 2 0.14156224198495693 1108 3 0.15650405811168255 1108 4 0.2573698207288757 1108 5 -0.0034348526342355967 1108 6 0.01285287497959331 1108 7 0.02607287731201069 1108 8 -0.047683973273784375 1108 9 0.01696135331587101 1108 10-11 0.172577610035745 1108 12-13 0.21136389495750763 1108 14-15 0.24005687807038356 1108 16-17 0.11744441516834314 1108 18-19 0.10461510922441875 1108 20-21 0.25687572109197276 1108 22-23 0.1132913321898954 1108 24-25 -0.01592958151017143 1108 26-27 0.10935380240317016 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2316 48-49 -0.5622866697067863 2316 50-51 -0.4284138708544205 2316 52-53 -0.4631955271503685 2316 54-55 -0.42020244643843085 2316 56-57 -0.48046187285593334 2316 58-59 -0.6389819004771482 2316 60-61 -0.3831300777306481 2316 62-63 -0.3013677210857537 2316 64-65 -0.5777073210856933 2316 66-67 -0.24289957757031644 2316 68-69 -0.7731689655329994 2316 70-71 -0.4394706378700519 2316 72-73 -0.38825234555542565 2316 74-75 -0.3997524798667982 2316 76-77 -0.4411235557765707 2316 78-79 -0.20560354089318977 2316 80-81 -0.29255771864240643 2316 82-83 -0.17185330530197263 2316 84-85 -0.26634408460639136 2316 86-87 -0.3925216565660463 2316 88-89 -0.43869762908617815 2316 90-91 -0.15998872295439526 2316 92-93 -0.07127643123288152 2316 94-95 0.7494154465627041 2316 96-97 0.4497053710945238 2316 98-99 0.9772892950388474 2316 100-101 1.438692312684914 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 6.0 4 5.0 5 7.0 6 31.0 7 92.0 8 173.0 9 294.0 10 489.0 11 851.0 12 1098.0 13 1508.0 14 1854.0 15 2526.0 16 3261.0 17 3933.0 18 3603.0 19 2894.0 20 2857.0 21 2851.0 22 3115.0 23 3472.0 24 3806.0 25 4331.0 26 4703.0 27 5602.0 28 6647.0 29 8100.0 30 9566.0 31 11569.0 32 13826.0 33 17141.0 34 21658.0 35 28028.0 36 36074.0 37 46278.0 38 31889.0 39 2201.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.16190542711462 21.78948103653 15.692882587134177 21.355730949221204 2 15.362200350093813 23.47235761279345 38.544909489219414 22.620532547893323 3 19.783823426695538 25.78996996577495 32.273870224208984 22.152336383320527 4 12.523224139135294 20.023747991897743 37.860934553328214 29.592093315638753 5 11.885767423649177 39.09064212999217 35.65205839579371 13.37153205056494 6 28.18781867709716 38.61353635538172 17.49598379548788 15.702661172033247 7 24.521198575120486 34.85122581546413 22.327652441153873 18.299923168261508 8 23.778026122791086 35.03981280994622 20.856673884193615 20.32548718306908 9 23.35126073898163 18.599217713208073 22.916462946147938 35.133058601662356 10-11 23.377104141929177 27.6915554934693 28.446776559335056 20.48456380526647 12-13 25.508661032339177 25.83606900887057 28.101906824055316 20.55336313473493 14-15 21.344555423622268 28.61545714884403 28.125305580778097 21.914681846755606 16-17 19.353565691136414 31.056087169099673 29.13948452888175 20.45086261088217 18-19 18.903750785779145 30.148075714185936 31.371271914507233 19.576901585527693 20-21 20.798351609974155 28.223440664943773 30.512153384088847 20.466054340993225 22-23 19.75326534888594 29.09303625061116 30.79328769993714 20.36041070056576 24-25 20.778597135178835 28.95309872509696 30.87077920830634 19.397524931417863 26-27 20.269427771597616 28.385092408416735 32.33290431192709 19.01257550805856 28-29 19.00188586994482 30.304183837396103 31.179017950687992 19.514912341971083 30-31 20.254068589788364 28.873891178319482 30.899105957952084 19.972934273940073 32-33 19.85230818661065 28.826096294782943 31.60898164591333 19.71261387269308 34-35 20.242750237947625 29.03100741348748 31.281773648501147 19.444468700063744 36-37 19.90420194774239 28.44657329005017 31.92680511924116 19.722419642966283 38-39 19.382352119366285 29.057461789935456 31.821055352203192 19.739130738495067 40-41 19.729257291140964 29.081964980171033 31.440835278976405 19.7479424497116 42-43 19.50448609815981 29.08821670176615 30.57339633777927 20.833900862294776 44-45 20.068137091408826 28.346217448473727 30.671295285758436 20.91435017435901 46-47 20.6712661019524 28.860496019585142 30.527573038298943 19.940664840163514 48-49 20.154361947335335 28.325941188796534 30.718551372494236 20.80114549137389 50-51 18.845603129147168 28.989662638820985 31.404973108891525 20.759761123140322 52-53 19.72305650625131 29.482258853111688 29.99563456031292 20.79905008032409 54-55 19.380980652371306 28.30586016623594 30.26559335056227 22.047565830830482 56-57 20.570824893483273 28.489732485855974 29.30292659076622 21.63651602989453 58-59 20.88304113990361 28.60969476845708 29.460955507438708 21.046308584200602 60-61 19.95844101417895 29.156073199692674 30.279213522385973 20.606272263742405 62-63 19.213522385974716 29.111371097296917 30.09743661381574 21.57766990291262 64-65 19.138786058531814 30.105643640427466 28.976740937347213 21.77882936369351 66-67 19.750855191128014 29.084356157638048 28.460271810211307 22.70451684102263 68-69 19.628273430665345 28.644493686690097 29.162235737647098 22.56499714499746 70-71 20.280261227910877 28.39875672277712 28.810854229237968 22.51012782007404 72-73 19.94220157854299 27.494063002025566 30.156806593560106 22.40692882587134 74-75 19.527834043444855 29.10473562897255 28.63099811413006 22.736432213452538 76-77 19.547216595655513 27.846965146329538 29.24198505273451 23.363833205280436 78-79 19.634874624572188 28.1305441084026 29.374869036809386 22.859712230215827 80-81 20.109834462527065 27.451107075504645 29.149612348955788 23.289446113012502 82-83 19.47485231640174 27.55218037392806 29.89975151787133 23.07321579179887 84-85 19.5225746741057 27.774314377766718 29.323065370947603 23.380045577179978 86-87 19.332881649530623 28.06510533080018 28.98919451274028 23.612818506928924 88-89 19.330549924128526 27.778738176185964 28.5007831612474 24.389928738438112 90-91 19.613393285895143 26.8915178766316 28.99969441655389 24.495394420919368 92-93 19.982608027547766 27.172512301852002 29.054547232476192 23.790332438124043 94-95 19.486449675211286 27.037612628343926 29.245302786896698 24.23063490954809 96-97 18.91862631596409 27.276886353437092 29.48196109862593 24.322526231972887 98-99 19.45571697981421 27.32695397080394 28.361213941468183 24.856115107913666 100-101 18.9984266927895 26.83143610993244 29.04524174974113 25.124895447536925 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 663.0 1 398.5 2 159.0 3 182.0 4 140.0 5 115.5 6 193.0 7 358.5 8 483.0 9 503.0 10 391.0 11 258.5 12 244.5 13 262.5 14 286.5 15 303.5 16 329.0 17 373.5 18 427.5 19 497.0 20 575.0 21 705.0 22 841.5 23 1028.5 24 1258.0 25 1516.0 26 1998.0 27 2602.0 28 3149.5 29 3719.5 30 4410.0 31 5210.0 32 6069.5 33 7002.0 34 8032.5 35 8717.0 36 9334.5 37 9920.5 38 10025.5 39 10059.5 40 10164.5 41 10249.0 42 10282.0 43 10487.5 44 10616.5 45 10591.5 46 10958.5 47 13145.5 48 16441.0 49 16022.0 50 12954.0 51 10300.5 52 8001.0 53 6542.0 54 5555.5 55 4757.0 56 3990.5 57 3255.5 58 2565.0 59 1925.5 60 1419.5 61 1048.5 62 731.5 63 497.5 64 356.5 65 276.0 66 216.0 67 154.5 68 101.0 69 77.0 70 56.0 71 51.0 72 43.0 73 35.0 74 22.0 75 10.5 76 11.5 77 8.0 78 3.5 79 2.5 80 1.5 81 1.0 82 0.5 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.045749807920653765 3 0.0 4 0.0 5 0.10197667109031222 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0015715582873507019 26-27 0.010302437661521268 28-29 0.0 30-31 0.0 32-33 1.7461758748341132E-4 34-35 0.01309631906125585 36-37 0.0029684989872179927 38-39 0.009778584899071035 40-41 0.005762380386952574 42-43 0.003841586924635049 44-45 0.0019207934623175246 46-47 1.7461758748341132E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.7461758748341132E-4 68-69 1.7461758748341132E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.23852762450234E-4 84-85 0.004365439687085283 86-87 0.0013969406998672906 88-89 1.7461758748341132E-4 90-91 8.730879374170566E-4 92-93 3.4923517496682265E-4 94-95 0.0 96-97 1.7461758748341132E-4 98-99 0.0 100-101 1.7461758748341132E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 286340.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.86681446026421 #Duplication Level Percentage of deduplicated Percentage of total 1 80.02300731002198 51.90837567731562 2 12.749761110418275 16.54072776724389 3 3.796675756285036 7.388347855461743 4 1.5076792395982033 3.9119339800243553 5 0.7173543930433436 2.326624715789901 6 0.3617320071071958 1.4078641793616866 7 0.21968656780519785 0.9975257483262414 8 0.1453408184656072 0.7542236723929187 9 0.09681331589230513 0.5651974259342194 >10 0.33233735184269564 3.5690380707273066 >50 0.023727540205289083 1.0560530257496914 >100 0.015099343767002144 2.2184459388223066 >500 0.006471147328715204 2.913503031291547 >1k 0.00431409821914347 4.442138911558573 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2894 1.0106865963539848 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1865 0.6513236013131243 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1737 0.6066214989173709 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1638 0.5720472165956555 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1230 0.42955926520919185 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1146 0.4002235105119788 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1104 0.3855556331633722 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1085 0.3789201648390026 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 869 0.3034853670461689 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 785 0.2741496123489558 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 782 0.27310190682405533 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 779 0.27205420129915486 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 755 0.2636725570999511 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 735 0.25668785360061463 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 674 0.2353845079276385 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 663 0.23154292100300342 TruSeq Adapter, Index 1 (95% over 21bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 654 0.22839980442830202 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 549 0.19173011105678564 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 545 0.19033317035691835 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 539 0.18823775930711742 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 465 0.16239435635957253 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 459 0.1602989453097716 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 450 0.1571558287350702 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 428 0.1494726548858001 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 372 0.12991548508765802 RNA PCR Primer, Index 1 (95% over 23bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 335 0.11699378361388559 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 308 0.10756443388978139 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 299 0.10442131731507998 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 294 0.10267514144024585 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 3.4923517496682265E-4 0.0 16-17 0.0 0.0 0.0 6.984703499336453E-4 0.0 18-19 0.0 0.0 0.0 6.984703499336453E-4 0.0 20-21 0.0 0.0 0.0 0.001047705524900468 0.0 22-23 0.0 0.0 0.0 0.004889292449535517 0.0 24-25 0.0 0.0 0.0 0.01169937836138856 0.0 26-27 0.0 0.0 0.0 0.024446462247677585 0.0 28-29 0.0 0.0 0.0 0.055528392819724805 0.0 30-31 0.0 0.0 0.0 0.11978766501362018 0.0 32-33 0.0 0.0 0.0 0.24952853251379478 0.0 34-35 0.0 0.0 0.0 0.4622127540685898 0.0 36-37 0.0 0.0 0.0 0.7592372703778725 0.0 38-39 0.0 0.0 0.0 1.09694768457079 0.0 40-41 0.0 0.0 0.0 1.4144024586156316 0.0 42-43 0.0 0.0 0.0 1.714220856324649 0.0 44-45 0.0 0.0 0.0 2.090347139763917 0.0 46-47 0.0 0.0 0.0 2.553956834532374 0.0 48-49 0.0 0.0 0.0 2.9599427254313055 0.0 50-51 0.0 0.0 0.0 3.335719773695607 0.0 52-53 0.0 0.0 0.0 3.710449116435007 0.0 54-55 0.0 0.0 0.0 4.17231263532863 0.0 56-57 0.0 0.0 0.0 4.726374240413495 0.0 58-59 0.0 0.0 0.0 5.247258503876511 0.0 60-61 0.0 0.0 0.0 5.765698121114759 0.0 62-63 0.0 0.0 0.0 6.278026122791088 0.0 64-65 0.0 0.0 0.0 6.840120136900188 0.0 66-67 0.0 0.0 0.0 7.501920793462317 0.0 68-69 0.0 0.0 0.0 8.20335964238318 0.0 70-71 0.0 0.0 0.0 8.944785918837745 0.0 72-73 0.0 0.0 0.0 9.642383180833974 0.0 74-75 0.0 0.0 0.0 10.404239715024097 0.0 76-77 0.0 0.0 0.0 11.190193476286932 0.0 78-79 0.0 0.0 0.0 12.10641195781239 0.0 80-81 0.0 0.0 0.0 13.021582733812949 0.0 82-83 0.0 0.0 0.0 13.919990221415102 0.0 84-85 0.0 0.0 0.0 14.836034085353077 0.0 86-87 0.0 0.0 0.0 15.783509115038067 0.0 88-89 0.0 0.0 0.0 16.808863588740657 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCCTAG 70 0.008349325 27.164082 1 GTAGTAC 70 0.008349325 27.164082 3 GACGTGA 135 1.3391058E-5 24.627365 7 GTGTAGC 165 2.402916E-6 23.048313 1 GTTCTAA 125 2.2382778E-4 22.81783 1 GAGTACT 1480 0.0 22.785067 12-13 GTATAGA 130 2.9185702E-4 21.940222 1 GTACTTT 1515 0.0 21.788425 14-15 TAGGACG 175 4.0044524E-6 21.731268 4 GGACGTG 175 4.034917E-6 21.712292 6 AGGACGT 185 6.479857E-6 20.556602 5 ACGTGAA 185 6.529048E-6 20.538652 8 CGTGAAA 190 8.222298E-6 19.998163 9 GTACATG 1095 0.0 19.96994 1 ACATGGG 1055 0.0 19.825918 3 ACATACC 120 0.0047334637 19.807144 3 GGCAGTA 120 0.0047574546 19.789848 7 TACATGG 1075 0.0 19.457064 2 GAATAAT 125 0.0059931986 19.014858 1 CTGTATA 150 7.7064824E-4 18.998253 9 >>END_MODULE