##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938456_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 287675 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94686364821413 31.0 31.0 34.0 28.0 34.0 2 31.10516033718606 31.0 31.0 34.0 28.0 34.0 3 31.133167637090466 31.0 31.0 34.0 27.0 34.0 4 34.78008864169636 37.0 35.0 37.0 32.0 37.0 5 34.798578256713306 37.0 35.0 37.0 32.0 37.0 6 34.795331537325104 37.0 35.0 37.0 32.0 37.0 7 34.738270617884766 37.0 35.0 37.0 32.0 37.0 8 34.7141670287651 37.0 35.0 37.0 32.0 37.0 9 36.17948031632919 39.0 35.0 39.0 32.0 39.0 10-11 36.05280611801512 39.0 35.0 39.0 31.5 39.0 12-13 35.92750673503085 38.0 35.0 39.0 30.5 39.0 14-15 36.99931867558877 39.0 36.0 41.0 31.0 41.0 16-17 36.96919440340662 39.0 36.0 41.0 31.0 41.0 18-19 37.019676718519165 39.5 36.0 41.0 31.0 41.0 20-21 36.99840966368298 39.0 36.0 41.0 31.0 41.0 22-23 36.8472703571739 39.0 36.0 41.0 31.0 41.0 24-25 36.72888850265056 39.0 36.0 41.0 30.5 41.0 26-27 36.51416355262015 39.0 36.0 41.0 30.0 41.0 28-29 36.306244894412096 39.0 35.5 40.5 30.0 41.0 30-31 36.07261666811506 39.0 35.0 40.0 29.5 41.0 32-33 35.74885200312853 38.0 35.0 40.0 28.5 41.0 34-35 35.466811506039804 38.0 35.0 40.0 27.0 41.0 36-37 35.236614234813594 38.0 34.5 40.0 26.5 41.0 38-39 35.009907013122444 38.0 34.0 40.0 25.5 41.0 40-41 34.80075953767272 38.0 34.0 40.0 25.0 41.0 42-43 34.41154253932389 38.0 33.5 40.0 24.0 41.0 44-45 33.94687755279395 38.0 33.0 40.0 22.5 41.0 46-47 33.43451985747805 38.0 33.0 40.0 18.5 41.0 48-49 32.96608325367168 37.5 32.0 40.0 14.5 41.0 50-51 32.2297783957591 36.5 30.5 39.5 9.0 40.5 52-53 32.318341878856344 37.0 31.0 39.0 9.0 40.0 54-55 32.428638220213784 37.0 31.5 40.0 7.5 41.0 56-57 32.057796124098374 37.0 31.0 40.0 2.0 41.0 58-59 31.69691839749718 37.0 31.0 39.5 2.0 41.0 60-61 31.20126010254628 36.0 29.5 39.0 2.0 40.5 62-63 30.610367602329017 36.0 28.5 39.0 2.0 40.0 64-65 30.06175371512992 35.0 28.0 39.0 2.0 40.0 66-67 29.44223516120622 35.0 27.5 38.0 2.0 40.0 68-69 28.684640653515252 34.0 26.0 38.0 2.0 40.0 70-71 27.933087685756497 34.0 25.5 37.0 2.0 39.0 72-73 27.125063005127316 33.0 23.0 36.5 2.0 39.0 74-75 26.287757017467626 32.0 20.5 36.0 2.0 38.5 76-77 25.40959589814895 31.5 17.5 35.0 2.0 38.0 78-79 24.48999913096376 31.0 10.0 35.0 2.0 37.0 80-81 23.547831754584166 30.0 4.5 34.0 2.0 36.0 82-83 22.617276440427567 29.5 2.0 34.0 2.0 36.0 84-85 21.672263839402103 29.0 2.0 34.0 2.0 35.0 86-87 20.760947249500305 27.5 2.0 34.0 2.0 35.0 88-89 19.68730511862345 26.0 2.0 33.0 2.0 35.0 90-91 18.512357695315895 25.0 2.0 32.0 2.0 34.0 92-93 17.32433649083167 21.0 2.0 32.0 2.0 34.0 94-95 16.014877900408447 11.0 2.0 31.0 2.0 34.0 96-97 14.536136264882245 2.0 2.0 31.0 2.0 34.0 98-99 12.756045885113409 2.0 2.0 30.0 2.0 34.0 100-101 9.054860519683672 2.0 2.0 13.5 2.0 29.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5506386833532702 1101 2 0.4687177435319363 1101 3 0.7444819613450591 1101 4 0.6190629145716855 1101 5 0.42869855938051415 1101 6 0.7621364358080172 1101 7 0.6463398861510683 1101 8 0.6708589679610313 1101 9 0.6226071603631738 1101 10-11 0.5792200450899756 1101 12-13 0.6595146111292465 1101 14-15 0.6981318624112021 1101 16-17 1.0949863449869355 1101 18-19 0.8234667299219538 1101 20-21 1.2206947030620654 1101 22-23 1.0538640429601998 1101 24-25 0.6993399383629253 1101 26-27 0.8470419640193398 1101 28-29 1.152946272507819 1101 30-31 0.9357384486410822 1101 32-33 1.070360669172537 1101 34-35 1.2413774538652547 1101 36-37 1.0822762766133422 1101 38-39 1.8625669332307737 1101 40-41 1.4092759225142686 1101 42-43 1.3755290049128845 1101 44-45 1.3591150205930091 1101 46-47 1.6248408856064387 1101 48-49 1.7437089533415104 1101 50-51 2.0240063787491493 1101 52-53 2.128432247624353 1101 54-55 2.395116564671497 1101 56-57 2.452475805911739 1101 58-59 2.000255318216972 1101 60-61 1.7480094957809236 1101 62-63 1.4220682309522594 1101 64-65 1.753032227578263 1101 66-67 2.16536240359072 1101 68-69 1.965887857911902 1101 70-71 2.022569380825889 1101 72-73 2.659775376755473 1101 74-75 2.8468206270793033 1101 76-77 2.9288199265901547 1101 78-79 3.009997241418148 1101 80-81 2.7422107181315702 1101 82-83 3.579424998615881 1101 84-85 3.1562586384152524 1101 86-87 3.4335084240354306 1101 88-89 3.335488290568609 1101 90-91 3.57186883151871 1101 92-93 3.960974240122077 1101 94-95 3.908917311472603 1101 96-97 4.849049107685341 1101 98-99 4.0187903713559745 1101 100-101 3.650217034069458 1102 1 0.951309432842919 1102 2 0.6279186767486173 1102 3 0.6528517484550029 1102 4 0.7396811706230082 1102 5 0.7028601225184516 1102 6 0.8329150884764331 1102 7 0.9968210760023339 1102 8 0.6035215518047181 1102 9 0.8580985572718944 1102 10-11 0.8400103629669076 1102 12-13 1.1009990089128578 1102 14-15 1.2620280420554124 1102 16-17 1.3434261233479674 1102 18-19 1.0289493779242918 1102 20-21 1.319061573869881 1102 22-23 1.143336192274866 1102 24-25 1.3612676140701296 1102 26-27 1.288613850866824 1102 28-29 0.9209836011750738 1102 30-31 1.1307224089793735 1102 32-33 1.2874210366266041 1102 34-35 1.0444833156325402 1102 36-37 1.3921333778090244 1102 38-39 1.4653374202532277 1102 40-41 1.5384097807819899 1102 42-43 1.674770766359373 1102 44-45 1.5090566945504307 1102 46-47 1.495619538274859 1102 48-49 1.6875555558495279 1102 50-51 1.7772288926015847 1102 52-53 1.448219357568945 1102 54-55 1.4916899096700433 1102 56-57 1.9555525046577245 1102 58-59 2.11862802162905 1102 60-61 2.0018152700591365 1102 62-63 1.809528131797986 1102 64-65 1.9877070598908908 1102 66-67 2.530468848618426 1102 68-69 2.3070222994400424 1102 70-71 2.11123954705511 1102 72-73 2.6279001944865925 1102 74-75 3.0167389706196985 1102 76-77 2.9073267799001563 1102 78-79 3.616077731356903 1102 80-81 3.7414670610886986 1102 82-83 3.693889857175229 1102 84-85 3.8099040160763806 1102 86-87 4.180942078161998 1102 88-89 4.864374263155373 1102 90-91 4.706820712533585 1102 92-93 4.991435015858446 1102 94-95 4.910740000303168 1102 96-97 4.395082936790038 1102 98-99 3.956789788095554 1102 100-101 3.544996853258727 1103 1 0.5703666719001568 1103 2 0.6172789461088861 1103 3 0.3868584824617365 1103 4 0.2272232581650968 1103 5 0.32494766460600033 1103 6 0.22402619958754144 1103 7 0.3893463285275871 1103 8 0.46204007032324057 1103 9 0.4894790286523616 1103 10-11 0.7063066592632055 1103 12-13 0.13581382372768047 1103 14-15 0.3347889848163561 1103 16-17 0.17854396846581722 1103 18-19 0.5941682331431366 1103 20-21 0.42131746612577814 1103 22-23 0.6200701990088717 1103 24-25 0.6203921931947036 1103 26-27 0.6977721000250696 1103 28-29 0.9426671028585716 1103 30-31 0.758365506622475 1103 32-33 0.5707543699599356 1103 34-35 0.39549341664263693 1103 36-37 0.5745239501995982 1103 38-39 0.6940916290074384 1103 40-41 0.9229552353274428 1103 42-43 0.8331882747768802 1103 44-45 0.13040349589722666 1103 46-47 0.6194579221132344 1103 48-49 0.6160067343007043 1103 50-51 0.2837272090998937 1103 52-53 0.5404079097574979 1103 54-55 0.6480872160673528 1103 56-57 0.7081450972503163 1103 58-59 0.9252946882957076 1103 60-61 0.40211830036216867 1103 62-63 0.6172722395420926 1103 64-65 0.9133972955811309 1103 66-67 1.3325563907059674 1103 68-69 1.5326451950629405 1103 70-71 1.8410375268530892 1103 72-73 1.8857453123317107 1103 74-75 1.5022608561415822 1103 76-77 1.8308958034691791 1103 78-79 1.8193100545892271 1103 80-81 1.931366050987691 1103 82-83 2.4144285777139523 1103 84-85 2.018456204628567 1103 86-87 2.2474740646939857 1103 88-89 2.960098168879277 1103 90-91 3.048840914553786 1103 92-93 2.742613467036893 1103 94-95 2.6568342763974435 1103 96-97 2.4057563374634388 1103 98-99 2.6538941651999277 1103 100-101 2.2364446647065392 1104 1 0.7460329234568199 1104 2 0.7399937655360347 1104 3 0.30952348868838797 1104 4 0.544906944341939 1104 5 0.34712699212779086 1104 6 0.5652590762998742 1104 7 0.5384496909186183 1104 8 0.7665606182684428 1104 9 0.8764902366224732 1104 10-11 0.8537724126878672 1104 12-13 0.719580947015352 1104 14-15 0.9193593459620573 1104 16-17 0.9149760979801442 1104 18-19 0.904000113591465 1104 20-21 0.8558068808206656 1104 22-23 0.7024051927822228 1104 24-25 0.7165441234562309 1104 26-27 1.0567011162143558 1104 28-29 1.0981652348635507 1104 30-31 1.2586270259250867 1104 32-33 1.0286310822887046 1104 34-35 0.9620251725579507 1104 36-37 1.4438203387425617 1104 38-39 1.309147668858003 1104 40-41 0.870944027357325 1104 42-43 1.3284871539798715 1104 44-45 0.9386102206792941 1104 46-47 1.1522785945914436 1104 48-49 1.1766854391574952 1104 50-51 1.2774445191870711 1104 52-53 1.4884527416379498 1104 54-55 1.6973088848095657 1104 56-57 1.464583453414697 1104 58-59 1.3890618115833888 1104 60-61 1.3486189230271712 1104 62-63 1.6231252906006226 1104 64-65 2.0136837214839964 1104 66-67 1.8415165401455695 1104 68-69 1.7991582710280696 1104 70-71 1.572388709916602 1104 72-73 1.958354278707798 1104 74-75 1.8447577428165474 1104 76-77 1.77417102140193 1104 78-79 1.744882906394956 1104 80-81 1.9903635603276655 1104 82-83 1.6385879799555418 1104 84-85 1.877877126172777 1104 86-87 1.8953383237226262 1104 88-89 2.7103534615318274 1104 90-91 2.2768359332337376 1104 92-93 2.208304625193808 1104 94-95 2.7705827197349286 1104 96-97 2.8882843573887147 1104 98-99 2.121503131576018 1104 100-101 1.381475797956849 1105 1 0.7663494728829576 1105 2 0.5285443023742431 1105 3 0.441465362068616 1105 4 0.5227392286810684 1105 5 0.6953776400359715 1105 6 0.8197018752632204 1105 7 0.6688180729993292 1105 8 0.7693373676205368 1105 9 0.6928082669816007 1105 10-11 0.9065769295334789 1105 12-13 0.6881111210249671 1105 14-15 0.8219880020153738 1105 16-17 0.7637589561808085 1105 18-19 0.8906485771234856 1105 20-21 0.9583383506466632 1105 22-23 0.9708011572398334 1105 24-25 1.052646492949009 1105 26-27 1.1456959329488967 1105 28-29 1.074724842416309 1105 30-31 1.0994035901605557 1105 32-33 1.3802138294193966 1105 34-35 1.1324713036205267 1105 36-37 1.5019932376688843 1105 38-39 1.4464810638968757 1105 40-41 1.463409780781987 1105 42-43 1.6144229185115293 1105 44-45 1.2821172551109896 1105 46-47 1.6857847034400137 1105 48-49 1.7412217220156947 1105 50-51 1.6315957594684463 1105 52-53 2.0239951333447195 1105 54-55 2.219893112873251 1105 56-57 1.9174018540070747 1105 58-59 1.9319163099173338 1105 60-61 1.6069954502393209 1105 62-63 1.2239675712374272 1105 64-65 2.125332185016017 1105 66-67 2.4912296093791824 1105 68-69 2.6406058830236248 1105 70-71 2.671750057565621 1105 72-73 2.7485708651572622 1105 74-75 2.6913886202693433 1105 76-77 3.121866319439693 1105 78-79 3.6117609145400884 1105 80-81 3.5615871812088216 1105 82-83 3.94514110842648 1105 84-85 3.699193305365668 1105 86-87 4.103039175259095 1105 88-89 3.842364753645864 1105 90-91 3.58482371553659 1105 92-93 3.8863173982408235 1105 94-95 3.6466008611640284 1105 96-97 3.081106460313558 1105 98-99 3.8306386369444017 1105 100-101 3.3796940504559245 1106 1 0.380409961943446 1106 2 0.4549783895226156 1106 3 -0.016913935596395646 1106 4 0.5830674140092569 1106 5 0.36597425706116127 1106 6 0.4467472880229195 1106 7 0.6312881961837391 1106 8 0.2730604784865065 1106 9 0.5187183607488421 1106 10-11 0.4458304687870154 1106 12-13 0.5617321910746185 1106 14-15 0.3329275129548819 1106 16-17 0.2113021626525864 1106 18-19 0.7544217891109852 1106 20-21 0.37510843705960184 1106 22-23 0.40724397761122333 1106 24-25 0.638289533949191 1106 26-27 0.48415428926440285 1106 28-29 0.34146735023025343 1106 30-31 0.20653495622050144 1106 32-33 0.737080900572181 1106 34-35 0.3548007759499967 1106 36-37 0.5572883286782613 1106 38-39 0.8128052962925381 1106 40-41 0.746573046088109 1106 42-43 0.6135840694583905 1106 44-45 1.1634028774680445 1106 46-47 1.0832612616308595 1106 48-49 0.8095132278072015 1106 50-51 0.37239140204980004 1106 52-53 0.8753924490277498 1106 54-55 0.8302764553994493 1106 56-57 1.1916825122163033 1106 58-59 0.4761110148263299 1106 60-61 0.5297064265217237 1106 62-63 0.7878350101048639 1106 64-65 1.0486292065273268 1106 66-67 0.7731748198958215 1106 68-69 1.0526761165224308 1106 70-71 1.1300604087331152 1106 72-73 1.3195857576007235 1106 74-75 0.9911909736431284 1106 76-77 0.8335550489855663 1106 78-79 1.7833112914476068 1106 80-81 1.601897900090968 1106 82-83 1.4833834323830892 1106 84-85 1.6493220054943691 1106 86-87 1.5976843306185344 1106 88-89 1.9298693500790307 1106 90-91 1.5900406671821123 1106 92-93 1.941623980333123 1106 94-95 2.099326546032568 1106 96-97 1.999807048657006 1106 98-99 1.0431087601288098 1106 100-101 0.5685040339087646 1107 1 0.7270216835551686 1107 2 0.660041183871126 1107 3 0.27514519574845053 1107 4 0.4846241905660307 1107 5 0.4894698091281384 1107 6 0.43889799445933875 1107 7 0.8139150930963552 1107 8 0.7060144292975963 1107 9 0.8256911498644826 1107 10-11 0.7869476849042272 1107 12-13 0.7786343365481869 1107 14-15 0.7924055349328967 1107 16-17 0.6720229894448408 1107 18-19 0.9175889790638934 1107 20-21 0.8389689812772829 1107 22-23 0.8601809216195946 1107 24-25 1.0688886966912037 1107 26-27 1.1903944776474447 1107 28-29 0.9661402963317656 1107 30-31 0.9472466255035954 1107 32-33 1.2348569340625062 1107 34-35 1.0109363070855295 1107 36-37 1.0018199874956366 1107 38-39 1.4349599273757363 1107 40-41 1.2454610628332716 1107 42-43 1.3579260370146429 1107 44-45 0.44306666606040324 1107 46-47 0.6814101365654537 1107 48-49 1.2007678065617782 1107 50-51 1.4830337359064174 1107 52-53 1.6282051125547028 1107 54-55 1.58488791536805 1107 56-57 1.6237861228913388 1107 58-59 1.8327305857316105 1107 60-61 1.8290588598027249 1107 62-63 1.694428416698269 1107 64-65 2.096182843366673 1107 66-67 2.0452836634332385 1107 68-69 2.378781558699302 1107 70-71 3.2680771538927154 1107 72-73 3.112266291352686 1107 74-75 3.4730067768875017 1107 76-77 3.8241358967092722 1107 78-79 4.137445252724426 1107 80-81 4.496488428610064 1107 82-83 4.666468204753574 1107 84-85 4.48059489926726 1107 86-87 4.499175696395614 1107 88-89 4.926304462585573 1107 90-91 3.7714574646703376 1107 92-93 3.873237010160441 1107 94-95 4.813873903437067 1107 96-97 3.6282738199809206 1107 98-99 2.7401587339644973 1107 100-101 2.9211008418627156 1108 1 0.1757396322731175 1108 2 0.3074770813070238 1108 3 0.17258109318434833 1108 4 0.3660344469762862 1108 5 -0.08745326779493467 1108 6 0.10556466112600305 1108 7 -0.08352080433954256 1108 8 0.14191186519503418 1108 9 0.16222961140294956 1108 10-11 -0.06401385355730582 1108 12-13 0.4036343365481869 1108 14-15 0.24272604775341478 1108 16-17 0.18644606636791394 1108 18-19 0.24931974829465275 1108 20-21 0.19153308384138512 1108 22-23 0.3233219472606237 1108 24-25 0.20510664540915968 1108 26-27 0.5397534520064227 1108 28-29 0.24819157838305017 1108 30-31 -0.1457020924451271 1108 32-33 0.13229283149840398 1108 34-35 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-1.028662273257524 2316 96-97 -0.04461269944025226 2316 98-99 -0.22755197941453176 2316 100-101 -0.6369246722271562 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2567.0 3 513.0 4 395.0 5 402.0 6 437.0 7 545.0 8 638.0 9 795.0 10 1046.0 11 1419.0 12 1873.0 13 2447.0 14 2716.0 15 3432.0 16 4198.0 17 4950.0 18 4481.0 19 4107.0 20 4420.0 21 4812.0 22 5407.0 23 5966.0 24 6522.0 25 7264.0 26 8151.0 27 9204.0 28 10605.0 29 11687.0 30 13527.0 31 15435.0 32 17593.0 33 20410.0 34 23490.0 35 26521.0 36 28751.0 37 23977.0 38 6857.0 39 115.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.304900688134225 20.92877182697542 15.35320966458691 21.413117820303448 2 15.318284863910097 23.007677222848084 38.74911336420912 22.924924549032696 3 20.185135600351913 25.387292965612207 31.472008846449423 22.955562587586456 4 12.402893208610077 19.12160517439232 38.66328198351706 29.81221963348054 5 12.157048249763482 38.81232956758863 35.38002949524181 13.650592687406087 6 28.66230076061364 38.021291617251705 17.841554475865212 15.474853146269446 7 24.924106226940637 34.71431601715042 22.579658034655548 17.781919721253388 8 23.905249724421633 34.03818803311809 21.328200794917535 20.728361447542746 9 23.932402594015684 18.42237947041744 23.307196133317106 34.33802180224977 10-11 23.45823901076929 27.4284800244804 28.63528724480748 20.477993719942834 12-13 25.568513727781024 25.96699490945506 28.181416729255332 20.283074633508583 14-15 21.627419789466366 28.45072626281065 28.51418190036596 21.40767204735703 16-17 19.422940856392344 30.606525771564964 29.63091092733825 20.33962244470444 18-19 19.021830781454003 30.173662240936583 31.40419717070847 19.40030980690094 20-21 20.904339843736416 28.380582146172863 30.64421348373211 20.070864526358616 22-23 19.8584470202072 28.90605033806162 30.91894692371751 20.316555718013667 24-25 20.567135425360263 28.986535358593446 30.812712123741164 19.633617092305126 26-27 20.246502748771658 28.240021141722565 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52-53 19.806217450859055 29.767384457068164 29.517594787965596 20.908803304107188 54-55 19.676721815280533 28.456406637301974 30.006744377657334 21.86012716976016 56-57 20.51035130108293 28.44269846512194 29.571868101305427 21.4750821324897 58-59 21.25493195209706 28.133766707802476 29.426589957068117 21.184711383032347 60-61 20.24606206827023 28.28357975463322 30.704221435002832 20.766136742093718 62-63 19.612008274375512 28.138613172944876 30.69237053905123 21.557008013628383 64-65 19.628743118676002 29.087988819532445 29.57435306428595 21.7089149975056 66-67 20.12480011124244 28.192657999026626 29.045400820412986 22.637141069317945 68-69 19.901375253775345 27.797090969769446 29.74716327836026 22.554370498094947 70-71 20.48827123475782 27.621828800876074 29.590210239963845 22.299689724402263 72-73 20.04185634998027 26.84612055254554 30.840203649915438 22.271819447558755 74-75 19.58313633329625 28.319508566025142 29.65742852375125 22.439926576927355 76-77 19.809672793638377 27.182375179246517 29.686351192804068 23.32160083431104 78-79 19.923551467175628 27.277942043042714 29.90201446577285 22.8964920240088 80-81 20.387502303250276 26.684327815962483 29.66082249501976 23.26734738576748 82-83 19.825300943027248 26.735561253313634 30.318717135283123 23.120420668375992 84-85 19.808757654821648 26.850411357034957 30.10076639474631 23.24006459339708 86-87 19.820417981840176 26.893154608785125 29.791634801229193 23.494792608145502 88-89 20.072765578717547 26.694155112711897 29.226522410268537 24.00655689830202 90-91 20.384339236571684 25.942029237458296 29.522913801825734 24.150717724144286 92-93 20.72397010255519 25.955675299843563 29.70797844602816 23.61237615157309 94-95 20.360748128201873 25.76327816302158 29.485318531627176 24.390655177149373 96-97 19.914265003189858 25.847050295431117 29.760925910152086 24.47775879122694 98-99 20.62561706506473 25.796274665220476 28.94087299931862 24.637235270396175 100-101 20.917865737687517 24.520734111620186 29.46765248894604 25.093747661746256 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 590.0 1 386.5 2 181.5 3 171.0 4 137.0 5 124.0 6 201.5 7 318.0 8 407.5 9 467.5 10 409.5 11 304.5 12 280.0 13 270.5 14 259.0 15 286.0 16 314.0 17 338.5 18 390.0 19 466.0 20 583.0 21 712.0 22 849.5 23 1047.5 24 1238.0 25 1550.5 26 2094.0 27 2707.5 28 3243.0 29 3806.5 30 4495.5 31 5225.0 32 5981.5 33 6806.5 34 7852.0 35 8716.5 36 9390.5 37 9961.0 38 10100.0 39 10091.5 40 10098.5 41 10134.0 42 10214.0 43 10264.5 44 10278.5 45 10327.5 46 11047.0 47 12754.0 48 15135.5 49 15363.5 50 12823.0 51 10208.5 52 8363.0 53 7111.0 54 5952.5 55 5051.5 56 4344.5 57 3592.5 58 2861.5 59 2215.5 60 1709.0 61 1255.5 62 905.5 63 714.0 64 552.0 65 437.5 66 336.5 67 245.5 68 193.5 69 157.5 70 124.0 71 88.0 72 78.5 73 70.0 74 53.0 75 43.5 76 31.0 77 21.5 78 17.0 79 11.5 80 10.0 81 8.5 82 3.0 83 2.5 84 2.5 85 0.5 86 1.0 87 1.0 88 1.0 89 1.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3834187885634831 2 0.024680629182236898 3 0.03545667854349527 4 0.036499522030068655 5 0.05944207873468324 6 0.050056487355522726 7 0.03545667854349527 8 0.034066220561397416 9 0.031285304597201705 10-11 0.034066220561397416 12-13 0.028852003128530458 14-15 0.025202050925523595 16-17 0.021378291474754497 18-19 0.026592508907621448 20-21 0.029199617624054923 22-23 0.02763535239419484 24-25 0.03719475102111758 26-27 0.031980533588250634 28-29 0.026592508907621448 30-31 0.029547232119579388 32-33 0.024854436429999134 34-35 0.025375858173285824 36-37 0.02763535239419484 38-39 0.012514121838880682 40-41 0.010776049361258365 42-43 0.011123663856782828 44-45 0.012166507343356218 46-47 0.020335447988181106 48-49 0.01842356826279656 50-51 0.01355696532545407 52-53 0.011471278352307292 54-55 0.009733205874684975 56-57 0.010428434865733902 58-59 0.003476144955244634 60-61 0.008342747892587122 62-63 0.013904579820978535 64-65 0.010602242113496134 66-67 0.005214217432866951 68-69 0.006604675414964804 70-71 0.009559398626922743 72-73 0.008516555140349354 74-75 0.009385591379160512 76-77 0.004345181194055792 78-79 0.010602242113496134 80-81 0.013209350829929608 82-83 0.013035543582167377 84-85 0.01025462761797167 86-87 0.002433301468671244 88-89 0.01355696532545407 90-91 0.022421134961327888 92-93 0.008690362388111585 94-95 0.017554532023985398 96-97 0.01546884505083862 98-99 0.007995133397062659 100-101 0.11627704875293299 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 287675.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.0710232833061 #Duplication Level Percentage of deduplicated Percentage of total 1 82.12531215500465 55.90354035852103 2 11.535860050767122 15.705155962174592 3 3.392131456665526 6.927175781001423 4 1.3068380416419616 3.5583121104048043 5 0.5933950481254467 2.0196504068572914 6 0.3443709570848952 1.4065010062692185 7 0.16595622284288739 0.7907766936403406 8 0.10768128055889122 0.5863979964880401 9 0.07854897438570974 0.4812218157860516 >10 0.30900830097483417 3.49239541161642 >50 0.018915099275984167 0.8501442996622378 >100 0.013802910282474863 2.256031721125774 >500 0.005112188993509208 2.5389491650985105 >1k 0.003067313396105525 3.4837472713543294 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2699 0.9382115234205266 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1761 0.61214912661858 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1634 0.5680020856869732 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1524 0.5297644911792821 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1232 0.4282610584861389 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1161 0.403580429303902 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 944 0.3281480837750934 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 812 0.2822629703658643 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 807 0.2805248978882419 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 774 0.26905361953593465 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 758 0.2634917876075432 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 724 0.25167289475971144 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 711 0.24715390631789347 No Hit GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 645 0.2242113496132789 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 620 0.21552098722516727 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 501 0.17415486225775614 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 495 0.17206917528460938 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 464 0.16129312592335102 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 450 0.1564265229860085 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 429 0.1491266185799948 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 416 0.14460763013817676 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 407 0.1414790996784566 No Hit TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 361 0.12548883288433127 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.10741287911705918 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 309 0.10741287911705918 No Hit ACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTG 295 0.1025462761797167 No Hit ATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATAC 289 0.1004605892065699 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.7380724776223168E-4 0.0 18-19 0.0 0.0 0.0 3.4761449552446336E-4 0.0 20-21 0.0 0.0 0.0 5.21421743286695E-4 0.0 22-23 0.0 0.0 0.0 0.004518988441818024 0.0 24-25 0.0 0.0 0.0 0.00938559137916051 0.0 26-27 0.0 0.0 0.0 0.016164074041887546 0.0 28-29 0.0 0.0 0.0 0.04692795689580255 0.0 30-31 0.0 0.0 0.0 0.11749369948726862 0.0 32-33 0.0 0.0 0.0 0.2523681237507604 0.0 34-35 0.0 0.0 0.0 0.4744937863908925 0.0 36-37 0.0 0.0 0.0 0.7904753628226298 0.0 38-39 0.0 0.0 0.0 1.1366994003649953 0.0 40-41 0.0 0.0 0.0 1.450769097071348 0.0 42-43 0.0 0.0 0.0 1.767445902494134 0.0 44-45 0.0 0.0 0.0 2.156078908490484 0.0 46-47 0.0 0.0 0.0 2.5806900147736163 0.0 48-49 0.0 0.0 0.0 2.981141913617798 0.0 50-51 0.0 0.0 0.0 3.352568002085687 0.0 52-53 0.0 0.0 0.0 3.7264273920222477 0.0 54-55 0.0 0.0 0.0 4.187885634830973 0.0 56-57 0.0 0.0 0.0 4.733466585556618 0.0 58-59 0.0 0.0 0.0 5.228643434431216 0.0 60-61 0.0 0.0 0.0 5.737377248631267 0.0 62-63 0.0 0.0 0.0 6.23133744677153 0.0 64-65 0.0 0.0 0.0 6.794125315025637 0.0 66-67 0.0 0.0 0.0 7.450073868080299 0.0 68-69 0.0 0.0 0.0 8.181280959416007 0.0 70-71 0.0 0.0 0.0 8.893021639002345 0.0 72-73 0.0 0.0 0.0 9.569827061788477 0.0 74-75 0.0 0.0 0.0 10.306769792300338 0.0 76-77 0.0 0.0 0.0 11.084557226036326 0.0 78-79 0.0 0.0 0.0 12.008690362388112 0.0 80-81 0.0 0.0 0.0 12.875640914226123 0.0 82-83 0.0 0.0 0.0 13.71113235421917 0.0 84-85 0.0 0.0 0.0 14.556009385591379 0.0 86-87 0.0 0.0 0.0 15.410793430086034 0.0 88-89 0.0 0.0 0.0 16.33544798818111 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTCTAGG 75 2.9978203E-4 31.800175 1 TGACGCG 30 0.0039455025 31.67293 94-95 GTAAGCG 45 2.4076244E-5 31.672928 94-95 TTCTCCG 35 0.008401413 27.134064 88-89 TACTGGA 95 0.001220427 25.013649 2 GTCCTAC 155 1.3539393E-6 24.61949 1 CCGTATC 90 1.9437903E-7 23.754696 94-95 GTACATG 950 0.0 22.092752 1 TAGGACG 110 0.0028630916 21.602695 4 CTACACT 200 5.329093E-7 21.386667 4 TCCTACA 225 7.0638634E-8 21.122637 2 ACATGGG 940 0.0 20.729395 3 GTACTCA 115 0.003708588 20.659853 6 ATCTAAG 140 4.7041223E-4 20.442968 1 TACATGG 955 0.0 20.403805 2 GTAGCCC 140 4.821786E-4 20.368258 3 TCAAGAC 140 4.821786E-4 20.368258 3 TACACTG 210 8.516872E-7 20.368256 5 CTGGACA 165 6.275221E-5 20.162516 4 AGGACGT 120 0.004739978 19.802471 5 >>END_MODULE