FastQCFastQC Report
Thu 26 May 2016
SRR938456_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938456_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences287675
Sequences flagged as poor quality0
Sequence length101
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT28030.9743634309550709No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT18260.6347440688276701No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16310.5669592422003998No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15690.5454071434778831No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT12170.4230468410532719No Hit
CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT12120.4213087685756496No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA11100.3858520900321543No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11050.384114017554532No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC8170.2840010428434866No Hit
ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC8070.2805248978882419No Hit
GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA8020.2787868254106196No Hit
GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG7560.2627965586164943No Hit
TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC7480.2600156426522986No Hit
TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT7390.2568871121925784No Hit
ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC6700.23290171200139048No Hit
ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT6490.22560180759537674TruSeq Adapter, Index 1 (95% over 21bp)
GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT6350.22073520465803426No Hit
GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA5140.17867385069957417No Hit
GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA5140.17867385069957417No Hit
TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT5100.1772833927174763No Hit
CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC5030.17485009124880507No Hit
AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC4660.16198835491439992No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4460.15503606500391068No Hit
GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG4300.14947423307551924No Hit
ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT3620.12583644737985572RNA PCR Primer, Index 1 (95% over 23bp)
TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC3310.11506039801859737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3300.11471278352307292No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3150.10949856609020596No Hit
GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG2940.10219866168419223No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCCGT250.005017531656.9997946
GTAAGCG354.3065447E-640.71413494-95
TCGTATC250.001615173937.99986394-95
CCTGTAC851.62801E-533.529293
AAGCGCA300.00394939731.66655268-69
GATAGTA804.4854562E-429.6873917
GTCCAAG1303.019013E-729.2357431
CATTCCG650.005836990729.2306619
CGGTAAA350.00838829927.14275714-15
TTATACT1050.00219229622.6189634
CTGTACT1503.0231098E-522.1665864
CTCCGAC1406.548362E-1122.0534994-95
GTATTAA1100.00286934521.5945851
ATAGCCC1353.7864994E-421.1110323
GTACTTT14300.021.09257914-15
GTGTAGC1604.966103E-520.7847881
AGTACTT14300.020.76041412-13
TCCAAGG1856.5144322E-620.5440352
TTAGGCA1404.8397048E-420.3570674
TAATAGT1404.8397048E-420.3570674
Produced by FastQC (version 0.11.5)