##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938456_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 287675 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.72996958373164 31.0 31.0 34.0 30.0 34.0 2 31.978082906057182 31.0 31.0 34.0 30.0 34.0 3 32.02098201094986 33.0 31.0 34.0 30.0 34.0 4 35.68326757625793 37.0 35.0 37.0 33.0 37.0 5 35.579066655079515 37.0 35.0 37.0 33.0 37.0 6 35.62360302424611 37.0 35.0 37.0 33.0 37.0 7 35.54997132180412 37.0 35.0 37.0 33.0 37.0 8 35.54234465977231 37.0 35.0 37.0 33.0 37.0 9 37.03514382549752 39.0 37.0 39.0 33.0 39.0 10-11 36.987431997914314 39.0 37.0 39.0 33.0 39.0 12-13 36.91038498305379 39.0 37.0 39.0 33.0 39.0 14-15 37.873798557399844 40.0 37.0 41.0 32.5 41.0 16-17 37.960698705136004 40.0 37.0 41.0 33.0 41.0 18-19 38.13407143477883 40.0 37.0 41.0 33.0 41.0 20-21 38.11555401060224 40.0 37.0 41.0 33.0 41.0 22-23 37.9890640479708 40.0 37.0 41.0 33.0 41.0 24-25 37.86258277570175 40.0 37.0 41.0 32.5 41.0 26-27 37.64502650560529 40.0 37.0 41.0 32.0 41.0 28-29 37.50574259146606 39.0 36.0 41.0 32.0 41.0 30-31 37.25662813939341 39.0 36.0 41.0 31.5 41.0 32-33 37.077514556357 39.0 36.0 41.0 31.0 41.0 34-35 36.90029199617624 39.0 35.0 41.0 31.0 41.0 36-37 36.75882158686017 39.0 35.0 41.0 30.5 41.0 38-39 36.5197827409403 39.0 35.0 40.0 30.0 41.0 40-41 36.361173198922394 39.0 35.0 40.0 30.0 41.0 42-43 36.05479447292952 38.5 35.0 40.0 30.0 41.0 44-45 35.74049535065612 38.0 35.0 40.0 29.0 41.0 46-47 35.27555574867472 38.0 35.0 40.0 26.5 41.0 48-49 34.840448422699225 38.0 34.0 40.0 25.0 41.0 50-51 34.44342052663596 38.0 33.5 40.0 24.0 41.0 52-53 33.94533935865125 38.0 33.0 40.0 23.0 41.0 54-55 33.60134005388025 38.0 33.0 40.0 21.5 41.0 56-57 33.21400191187972 37.0 32.5 40.0 19.0 41.0 58-59 32.74629356044147 37.0 31.5 39.5 16.0 41.0 60-61 32.26243156339619 36.0 31.0 39.0 12.0 40.0 62-63 31.87339880072999 36.0 31.0 39.0 8.0 40.0 64-65 31.319734074910926 35.5 30.0 39.0 4.5 40.0 66-67 30.66367080907274 35.0 29.0 38.5 2.0 40.0 68-69 30.002249065786046 34.5 28.0 38.0 2.0 40.0 70-71 29.297365082123925 34.0 27.0 37.5 2.0 39.0 72-73 28.763323194577215 34.0 26.0 36.5 2.0 39.0 74-75 28.124440775180325 33.0 26.0 36.0 2.0 39.0 76-77 26.946583818545236 31.5 25.5 34.5 2.0 37.0 78-79 27.50061527765708 33.0 26.0 35.0 2.0 37.0 80-81 27.150977665768664 33.0 26.0 35.0 2.0 37.0 82-83 26.555896410880333 32.0 24.5 35.0 2.0 36.5 84-85 25.876702876509952 32.0 22.0 35.0 2.0 36.0 86-87 25.108638220213784 31.0 19.0 34.0 2.0 35.5 88-89 24.256492569740157 31.0 15.5 34.0 2.0 35.0 90-91 23.53084904840532 30.0 7.0 34.0 2.0 35.0 92-93 22.595222038759015 29.5 2.0 34.0 2.0 35.0 94-95 21.556883636047623 29.0 2.0 34.0 2.0 35.0 96-97 20.275939862692276 28.0 2.0 33.5 2.0 35.0 98-99 18.586036325714783 25.5 2.0 33.0 2.0 34.0 100-101 14.184944816198835 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3384377563960932 1101 2 0.365917517442341 1101 3 0.42064753466766547 1101 4 0.14517303234006107 1101 5 0.020134169041128303 1101 6 0.12431217172314035 1101 7 0.3419055900315797 1101 8 0.44321233530411774 1101 9 0.5435043650778368 1101 10-11 0.3619124292890561 1101 12-13 0.40979333798139805 1101 14-15 0.8143572451378418 1101 16-17 0.5188491655799581 1101 18-19 0.405223315595876 1101 20-21 0.6763861165461549 1101 22-23 0.8799360019486642 1101 24-25 1.0104192180241256 1101 26-27 0.917723251999945 1101 28-29 0.806898706585045 1101 30-31 1.0100060653639744 1101 32-33 0.8974123971783712 1101 34-35 0.719662465024399 1101 36-37 0.8691517542122895 1101 38-39 1.0455886275853246 1101 40-41 1.1913876517990545 1101 42-43 1.4943527792333242 1101 44-45 1.1717692465870542 1101 46-47 1.121602672443231 1101 48-49 1.3298787207123937 1101 50-51 1.4736860494960453 1101 52-53 1.0590141698584858 1101 54-55 0.8407101555068337 1101 56-57 1.3289076175874897 1101 58-59 1.386724657796627 1101 60-61 1.1823347054276425 1101 62-63 1.8670747710332627 1101 64-65 1.308764981924579 1101 66-67 1.7127330489849264 1101 68-69 1.9063698682386203 1101 70-71 2.2333753418696283 1101 72-73 2.0554809675966617 1101 74-75 1.9141421032610033 1101 76-77 2.526614050621692 1101 78-79 2.420344442485156 1101 80-81 2.594600639918955 1101 82-83 2.6805468967757733 1101 84-85 2.544414554035093 1101 86-87 2.474380159056615 1101 88-89 2.722374808506924 1101 90-91 3.01209516317628 1101 92-93 3.3478196068784136 1101 94-95 3.370632086820997 1101 96-97 3.0113235330996915 1101 98-99 2.511192747929517 1101 100-101 2.0911669366800414 1102 1 0.27704959658273864 1102 2 0.354631428203497 1102 3 0.3479732856154669 1102 4 0.3081943213456029 1102 5 0.39275009205075406 1102 6 0.22349560712704886 1102 7 0.4438011864153637 1102 8 0.2885899964298133 1102 9 0.0441459515461915 1102 10-11 0.3522448877317501 1102 12-13 0.309399637193998 1102 14-15 0.28045523288936636 1102 16-17 0.45738809821337867 1102 18-19 0.45147586736023726 1102 20-21 0.23017730931372427 1102 22-23 0.2725723390731858 1102 24-25 0.30640930259689014 1102 26-27 0.37483902919446166 1102 28-29 -0.14893389767275522 1102 30-31 -0.018617440031185595 1102 32-33 -0.22285590261748922 1102 34-35 0.36458809932010894 1102 36-37 0.47599048270456024 1102 38-39 0.6995693799912743 1102 40-41 0.8822013000930156 1102 42-43 1.0145627529866061 1102 44-45 0.8784038339302995 1102 46-47 0.8347114505126427 1102 48-49 0.38232841450066246 1102 50-51 0.4433710888661224 1102 52-53 0.6580663716665924 1102 54-55 0.8885229289101559 1102 56-57 1.2649968564326315 1102 58-59 1.4888389768400785 1102 60-61 1.4703049591459276 1102 62-63 1.8303731087410462 1102 64-65 1.6181700562902783 1102 66-67 1.584182451142997 1102 68-69 1.6970085384048517 1102 70-71 1.7906223526599483 1102 72-73 1.9557580162989048 1102 74-75 1.7047078658740098 1102 76-77 1.7574976901667512 1102 78-79 1.7034298901375315 1102 80-81 1.3442215206422006 1102 82-83 1.6777472467320287 1102 84-85 2.2138662892348613 1102 86-87 2.1540535332181747 1102 88-89 2.259149378352358 1102 90-91 2.047440744978555 1102 92-93 1.714223806353477 1102 94-95 1.1864967704022185 1102 96-97 1.1870890624085284 1102 98-99 1.684727306109746 1102 100-101 2.085800940762864 1103 1 0.1309216504547912 1103 2 0.30796476153683017 1103 3 0.2206332182753954 1103 4 -0.008842715691436354 1103 5 0.13052786982852638 1103 6 0.10962355325499828 1103 7 0.21093923355341104 1103 8 0.244414912254733 1103 9 0.2675129549131938 1103 10-11 0.34547721096407713 1103 12-13 0.22667236446672945 1103 14-15 0.17691987935401698 1103 16-17 0.18203456285984743 1103 18-19 0.3743714902558608 1103 20-21 0.10899885813527277 1103 22-23 0.17806055456140513 1103 24-25 0.22082007700766582 1103 26-27 0.1823474467028774 1103 28-29 0.029382600643742762 1103 30-31 -0.06767467908841951 1103 32-33 0.25697574721416316 1103 34-35 0.13085072558273936 1103 36-37 0.5844416611557435 1103 38-39 0.5321956426175376 1103 40-41 0.7043225122142331 1103 42-43 0.8033506317744852 1103 44-45 0.31611427164073547 1103 46-47 0.02656330236449378 1103 48-49 0.25758093975319696 1103 50-51 0.35700745250247934 1103 52-53 0.5117700753702934 1103 54-55 0.31720979759702317 1103 56-57 0.6471180685538442 1103 58-59 0.7436537916549 1103 60-61 1.0671736460146164 1103 62-63 0.8660296743976126 1103 64-65 0.7200892482094723 1103 66-67 0.7716235285840689 1103 68-69 0.706806518202832 1103 70-71 0.37510046713806133 1103 72-73 0.7720879826288716 1103 74-75 1.178916620082763 1103 76-77 0.6234572861263459 1103 78-79 0.3103659170735611 1103 80-81 0.5957030021236811 1103 82-83 0.6723937113784935 1103 84-85 0.6310716764402464 1103 86-87 0.446141075305718 1103 88-89 -0.039099779896798026 1103 90-91 1.0694609469987562 1103 92-93 1.3736177457474206 1103 94-95 1.0556213495267919 1103 96-97 1.3285032038226703 1103 98-99 1.580383871766312 1103 100-101 0.5978884828504061 1104 1 0.2639851622854259 1104 2 0.1591976382491609 1104 3 0.29358465040490955 1104 4 0.36704769526746617 1104 5 0.2917607465408594 1104 6 0.12423127430107428 1104 7 0.22748966941891524 1104 8 0.250056257211142 1104 9 0.29234483536151146 1104 10-11 0.11529663811226953 1104 12-13 0.36076950020769516 1104 14-15 0.6116957199517685 1104 16-17 0.44102584554975266 1104 18-19 0.47683101703045594 1104 20-21 0.33454057669069215 1104 22-23 0.36825980736339403 1104 24-25 0.10242655272372758 1104 26-27 0.5292403483218067 1104 28-29 0.34032282480314535 1104 30-31 0.17124811045081145 1104 32-33 0.523706755931471 1104 34-35 0.8600599410248293 1104 36-37 0.6185450235467727 1104 38-39 0.9385343723809214 1104 40-41 0.9721556379925573 1104 42-43 0.6119371822103474 1104 44-45 0.5795327025249222 1104 46-47 0.7773167270220256 1104 48-49 0.9208748376361342 1104 50-51 0.7810423217428308 1104 52-53 0.7532084315346737 1104 54-55 1.3115061861399866 1104 56-57 1.6224169477568324 1104 58-59 1.299544202613795 1104 60-61 1.1809594492524695 1104 62-63 1.6223061376603773 1104 64-65 1.822355748832134 1104 66-67 2.2009759569775973 1104 68-69 1.7287056464718233 1104 70-71 1.4143159092302149 1104 72-73 1.813152739789519 1104 74-75 1.5310523610541225 1104 76-77 1.5195220432869938 1104 78-79 1.9294319195642196 1104 80-81 2.071935878836012 1104 82-83 2.1607000625615562 1104 84-85 1.935162585531156 1104 86-87 2.032909443923405 1104 88-89 1.7633223869774248 1104 90-91 2.038346376637609 1104 92-93 1.9401370483626117 1104 94-95 1.5304719099253 1104 96-97 1.7932914977205527 1104 98-99 2.5804274583167484 1104 100-101 2.010889728180418 1105 1 0.38866120819434613 1105 2 0.27850530207737023 1105 3 0.1571074197496003 1105 4 -0.04303190488062114 1105 5 -0.0585261842255278 1105 6 0.1814785901100322 1105 7 0.12220458981877158 1105 8 0.14477117761099834 1105 9 0.2874392448394829 1105 10-11 0.360090233077095 1105 12-13 0.27561585341021555 1105 14-15 0.32587565330979373 1105 16-17 0.37105176187704814 1105 18-19 0.3495864779708526 1105 20-21 0.4295516836881035 1105 22-23 0.08253229903315429 1105 24-25 0.3794073005948846 1105 26-27 0.2185452354006685 1105 28-29 0.29968972595086996 1105 30-31 0.6781668442531057 1105 32-33 0.8830875408259544 1105 34-35 0.8063298410618529 1105 36-37 0.5124269191409994 1105 38-39 0.6237435541654435 1105 40-41 0.8590031018948139 1105 42-43 0.6326933836172373 1105 44-45 0.5413292593557273 1105 46-47 0.9264281672293606 1105 48-49 0.8965551412273953 1105 50-51 0.7602629807580072 1105 52-53 0.4881214267216478 1105 54-55 0.08589530128252676 1105 56-57 0.9015959555317323 1105 58-59 1.0093970348981358 1105 60-61 0.8433530071939757 1105 62-63 1.0796598955278363 1105 64-65 1.4311580442782734 1105 66-67 1.2089822509427925 1105 68-69 1.4071812110775213 1105 70-71 1.3852294597670536 1105 72-73 1.7603250833659736 1105 74-75 1.7912569149230606 1105 76-77 1.7623024949715536 1105 78-79 2.2447086689163136 1105 80-81 1.7841489480696247 1105 82-83 1.9255325320173142 1105 84-85 2.2358382612068333 1105 86-87 1.8239359156005577 1105 88-89 1.6199727016756853 1105 90-91 1.7284842885220968 1105 92-93 1.968390473020147 1105 94-95 1.792064838470285 1105 96-97 1.4790560293754922 1105 98-99 0.5367167955992365 1105 100-101 1.0766292690911907 1106 1 -0.008286760182190989 1106 2 -0.03584476227269562 1106 3 0.025902234972988936 1106 4 0.1317015019956429 1106 5 0.07801086302580984 1106 6 -0.12246055311481996 1106 7 0.1982985409127167 1106 8 0.24127329197025205 1106 9 -0.12509063197610715 1106 10-11 0.06505667911497426 1106 12-13 -0.0069268934182460384 1106 14-15 -0.0891592795822902 1106 16-17 -0.031274033305898286 1106 18-19 0.15427254158548465 1106 20-21 -0.12443342386843881 1106 22-23 0.15639698004068947 1106 24-25 0.2265529155976438 1106 26-27 -0.16457896022352259 1106 28-29 0.0026911968094793792 1106 30-31 0.15602352746692816 1106 32-33 0.27434742315726623 1106 34-35 0.3015495505672803 1106 36-37 0.19182570568264623 1106 38-39 0.41952402851735116 1106 40-41 0.07845980349438264 1106 42-43 0.2975559502655116 1106 44-45 0.3749268875962102 1106 46-47 -0.33214418062870976 1106 48-49 -0.2954493632771076 1106 50-51 -0.02363571385496499 1106 52-53 0.11040953115261232 1106 54-55 -0.5163523545365578 1106 56-57 -0.45813239526805205 1106 58-59 0.03358576444401251 1106 60-61 0.1640691314815257 1106 62-63 -0.40171305905940713 1106 64-65 -0.21981180312014814 1106 66-67 -0.09091203109434431 1106 68-69 -0.18825223257020696 1106 70-71 -0.3256663850573638 1106 72-73 0.44812385152188483 1106 74-75 0.5055771024575328 1106 76-77 0.9359557400533731 1106 78-79 0.7913739566530289 1106 80-81 0.6277438184502095 1106 82-83 0.8901055233760182 1106 84-85 0.721727211381495 1106 86-87 1.2678479398697249 1106 88-89 1.0057858107030775 1106 90-91 1.6901845091508925 1106 92-93 1.1805503369657266 1106 94-95 1.4019540644309387 1106 96-97 1.858744391206713 1106 98-99 2.5329132471528304 1106 100-101 2.6689831025164548 1107 1 0.25603820057134286 1107 2 0.2571955307675964 1107 3 0.2936285562707326 1107 4 0.16218292533420708 1107 5 0.26796376726442617 1107 6 0.10091725954870157 1107 7 0.1254963431105196 1107 8 0.33075523859505296 1107 9 0.3876644700647134 1107 10-11 0.1130354860223477 1107 12-13 0.259720150014509 1107 14-15 0.3247928397269746 1107 16-17 0.0706359614612424 1107 18-19 0.2402223061066806 1107 20-21 0.37017018680660385 1107 22-23 0.29814213964299086 1107 24-25 0.347399331086919 1107 26-27 0.446455838311266 1107 28-29 0.352704278965426 1107 30-31 0.30663184771810137 1107 32-33 0.5073079150463329 1107 34-35 0.33916074889275905 1107 36-37 0.4340745282886118 1107 38-39 0.3491420295639216 1107 40-41 0.6501500180417352 1107 42-43 0.8562294196532676 1107 44-45 0.5377343182607817 1107 46-47 0.981435097236286 1107 48-49 0.9946147392869946 1107 50-51 1.3539480119430394 1107 52-53 1.2297187933190088 1107 54-55 1.399954552841777 1107 56-57 1.4226891641249395 1107 58-59 1.5514102019113025 1107 60-61 1.622762224103191 1107 62-63 1.5498175531854947 1107 64-65 1.3128281759484075 1107 66-67 0.7357494027099456 1107 68-69 1.4647435811398921 1107 70-71 1.5135212130588052 1107 72-73 2.206790779831671 1107 74-75 1.964073962740109 1107 76-77 1.8698908525599158 1107 78-79 1.9265780382856796 1107 80-81 2.0497414636621407 1107 82-83 2.3095848535696355 1107 84-85 2.029713867582437 1107 86-87 1.9743170659817117 1107 88-89 1.8076892643922484 1107 90-91 2.3489364714742784 1107 92-93 2.617428934558607 1107 94-95 2.7617460581515054 1107 96-97 2.4677514555709195 1107 98-99 2.4692358531182954 1107 100-101 2.2794619094238318 1108 1 0.054115123648266206 1108 2 -0.11780446923240007 1108 3 -0.030089392447209207 1108 4 -0.13589399774271982 1108 5 -0.06536956606890953 1108 6 -0.11382633019488253 1108 7 0.032547625161804206 1108 8 -0.02180886396904924 1108 9 0.17933113673137768 1108 10-11 0.1402790757659389 1108 12-13 -0.06720292690857121 1108 14-15 -0.029373826939689707 1108 16-17 -0.2130178846926043 1108 18-19 -0.0947135913292172 1108 20-21 -0.20675289011647635 1108 22-23 0.14269342169427546 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-0.5527106250282543 2316 48-49 0.4529004717063856 2316 50-51 -0.2706553137741423 2316 52-53 0.25104400276303096 2316 54-55 0.6946835449717597 2316 56-57 0.13974933168015014 2316 58-59 0.11784521079680843 2316 60-61 0.4786107897289824 2316 62-63 0.3406591373439092 2316 64-65 0.12537577686233803 2316 66-67 0.2538377500062161 2316 68-69 0.5877126088118914 2316 70-71 0.6072156786592124 2316 72-73 0.648955669397548 2316 74-75 0.7597867070914681 2316 76-77 0.7922061610138371 2316 78-79 0.47732061254310665 2316 80-81 -0.1681913873152645 2316 82-83 0.6110255745648132 2316 84-85 0.5502615954321435 2316 86-87 -0.0012636651683273215 2316 88-89 0.5432344535265479 2316 90-91 0.030169017805835807 2316 92-93 0.6901313971125553 2316 94-95 0.6292727146633084 2316 96-97 0.7391242659288793 2316 98-99 0.3524390772868635 2316 100-101 -0.27248655465334437 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 6.0 5 16.0 6 44.0 7 81.0 8 183.0 9 317.0 10 562.0 11 815.0 12 1176.0 13 1525.0 14 2047.0 15 2693.0 16 3467.0 17 4240.0 18 4051.0 19 3248.0 20 3254.0 21 3462.0 22 3843.0 23 4089.0 24 4751.0 25 5217.0 26 5880.0 27 6804.0 28 7913.0 29 9687.0 30 11271.0 31 13254.0 32 15818.0 33 19133.0 34 23785.0 35 30252.0 36 37430.0 37 39988.0 38 16916.0 39 454.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.830496747342764 21.737694316281367 15.859268247737395 21.572540688638473 2 15.347415849088089 23.47846946509154 38.61833276548995 22.555781920330414 3 19.941113490364025 25.59998887622014 32.22698072805139 22.231916905364443 4 12.53741201008082 19.951333970626575 37.99947857825671 29.511775441035894 5 11.828278439210916 39.171287042669675 35.65968540888155 13.340749109237853 6 28.384809246545583 38.725645259407315 17.471452159555053 15.418093334492047 7 24.7289475971148 34.64847484140089 22.305031719822715 18.317545841661598 8 23.73581298340141 35.05900756061528 20.921178413139828 20.284001042843485 9 23.374375160772264 18.469865194630035 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19.804535689704316 34-35 20.28760546634637 29.097239362978055 31.05238639879576 19.562768771879814 36-37 19.90562184428473 28.41130104545968 31.85949299985226 19.82358411040332 38-39 19.356661104157975 28.912181894034212 31.86900292031706 19.86215408149075 40-41 19.878703388239547 29.069989778961347 31.24004839348913 19.811258439309974 42-43 19.559417307314597 28.95039186274288 30.629257346665995 20.860933483276526 44-45 20.039523905531954 28.350146346211336 30.71532359545875 20.89500615279796 46-47 20.7431997914313 28.717476318762493 30.560528374033197 19.978795515773008 48-49 20.085686973146778 28.381159294342574 30.65195098635613 20.881202746154514 50-51 18.893716867993398 29.008777266011993 31.28269748848527 20.81480837750934 52-53 19.741896237073085 29.42695750412792 29.960719562005732 20.870426696793256 54-55 19.390457982097853 28.21969236117146 30.24558964108803 22.144260015642654 56-57 20.672807856087598 28.385678282784394 29.279395150777788 21.662118710350224 58-59 20.87720517945598 28.558790301555575 29.467802207352044 21.096202311636393 60-61 20.05996350047797 29.171634657165203 30.075084731033286 20.69331711132354 62-63 19.250369340401495 29.12070913357087 30.015642652298602 21.613278873729033 64-65 19.11844963934996 30.152602763535242 28.972972972972972 21.755974624141828 66-67 19.89397573608649 29.074807939653073 28.34810720617374 22.683109118086698 68-69 19.598222277841217 28.71469714587646 29.09933726490111 22.58774331138121 70-71 20.37472842617537 28.35039541148866 28.873381420005213 22.401494742330755 72-73 19.996523855044757 27.55470583123316 29.993395324585038 22.45537498913705 74-75 19.590162509776658 28.86781958807682 28.717823933258018 22.824193968888505 76-77 19.455983314504216 27.864951768488744 29.309811419136178 23.36925349787086 78-79 19.6682019640219 27.93916746328322 29.46502129138785 22.927609281307028 80-81 20.036847136525594 27.455635700008692 29.234900495350658 23.27261666811506 82-83 19.411203403882553 27.437024522762943 29.887910342178493 23.26386173117601 84-85 19.58450371276803 27.713657980365436 29.4195149762216 23.282323330644935 86-87 19.458151625075825 27.9248149313347 29.12899968192002 23.48803376166945 88-89 19.3861128009038 27.794212218649516 28.341705049100547 24.477969931346138 90-91 19.75274094982515 26.96211684059039 28.891036381459568 24.39410582812489 92-93 19.92564448399566 27.264732055952614 28.964410856809142 23.845212603242583 94-95 19.508680818077377 26.995093412867664 28.978932786882396 24.51729298217256 96-97 18.93994959589815 27.081254888328843 29.509342139567217 24.469453376205788 98-99 19.512296862779177 27.209872251672895 28.375076040670894 24.90275484487703 100-101 19.033979316937515 26.80281567741375 28.96480403232815 25.198400973320588 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 630.0 1 389.0 2 172.0 3 183.0 4 125.0 5 96.0 6 181.5 7 364.5 8 482.0 9 480.5 10 393.5 11 267.0 12 237.0 13 259.0 14 268.0 15 271.0 16 301.0 17 368.5 18 434.0 19 500.0 20 579.5 21 686.0 22 826.5 23 1009.0 24 1245.0 25 1527.5 26 1986.0 27 2567.0 28 3145.0 29 3820.5 30 4437.0 31 5201.5 32 6059.5 33 6890.5 34 8018.0 35 8804.0 36 9432.0 37 9966.0 38 10012.5 39 10180.0 40 10290.0 41 10275.5 42 10353.5 43 10417.0 44 10470.5 45 10556.5 46 11188.5 47 13375.0 48 16272.0 49 16040.0 50 13133.5 51 10492.0 52 8221.0 53 6607.5 54 5653.0 55 4851.5 56 4044.0 57 3252.0 58 2548.0 59 1928.0 60 1404.5 61 1050.5 62 756.0 63 521.0 64 379.5 65 304.0 66 234.0 67 163.5 68 120.5 69 85.5 70 55.0 71 41.5 72 28.0 73 23.5 74 18.0 75 12.0 76 12.0 77 7.5 78 5.5 79 3.0 80 0.5 81 0.0 82 0.0 83 0.0 84 0.0 85 0.0 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.022247327713565655 2 0.012861736334405145 3 0.0010428434865733902 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 3.4761449552446336E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0019118797253845487 26-27 0.008690362388111585 28-29 1.7380724776223168E-4 30-31 0.0 32-33 3.4761449552446336E-4 34-35 0.008342747892587122 36-37 8.690362388111585E-4 38-39 0.012166507343356218 40-41 0.011471278352307292 42-43 0.0036499522030068654 44-45 3.4761449552446336E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.001738072477622317 68-69 0.001216650734335622 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 0.001216650734335622 84-85 0.006604675414964804 86-87 0.003997566698531329 88-89 0.0 90-91 3.4761449552446336E-4 92-93 0.0010428434865733902 94-95 1.7380724776223168E-4 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 287675.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.89130382748007 #Duplication Level Percentage of deduplicated Percentage of total 1 79.99582938358361 51.910336694613825 2 12.667544538693853 16.440269628170373 3 3.8987435917189317 7.589836648670224 4 1.5373733923285282 3.99048655591497 5 0.7001515966898295 2.271687499304751 6 0.38106784856840437 1.4836793724201898 7 0.2143736038760796 0.9737687863200178 8 0.12685915698462222 0.6585644879349689 9 0.08613066840119815 0.5030218234877478 >10 0.3414811550475811 3.6789446366909777 >50 0.025222532053673866 1.1087927665289117 >100 0.013952890072245107 2.029884747517909 >500 0.006976445036122553 3.0171421260070614 >1k 0.004293196945306187 4.343584226418061 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 2803 0.9743634309550709 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1826 0.6347440688276701 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1631 0.5669592422003998 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1569 0.5454071434778831 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1217 0.4230468410532719 No Hit CTTATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGT 1212 0.4213087685756496 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1110 0.3858520900321543 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1105 0.384114017554532 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 817 0.2840010428434866 No Hit ACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGC 807 0.2805248978882419 No Hit GGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTA 802 0.2787868254106196 No Hit GTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGG 756 0.2627965586164943 No Hit TATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATC 748 0.2600156426522986 No Hit TGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCT 739 0.2568871121925784 No Hit ACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTC 670 0.23290171200139048 No Hit ATACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTT 649 0.22560180759537674 TruSeq Adapter, Index 1 (95% over 21bp) GTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTAT 635 0.22073520465803426 No Hit GATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATA 514 0.17867385069957417 No Hit GTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAA 514 0.17867385069957417 No Hit TGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGAT 510 0.1772833927174763 No Hit CCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCAC 503 0.17485009124880507 No Hit AAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGC 466 0.16198835491439992 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 446 0.15503606500391068 No Hit GTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTG 430 0.14947423307551924 No Hit ACACATCTCCGAGCCCACGAGACTAGGCATGATCTCGTATGCCGTCTTCT 362 0.12583644737985572 RNA PCR Primer, Index 1 (95% over 23bp) TACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACC 331 0.11506039801859737 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 330 0.11471278352307292 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 315 0.10949856609020596 No Hit GCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGTAAGCAG 294 0.10219866168419223 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 1.7380724776223168E-4 0.0 18-19 0.0 0.0 0.0 3.4761449552446336E-4 0.0 20-21 0.0 0.0 0.0 5.21421743286695E-4 0.0 22-23 0.0 0.0 0.0 0.004518988441818024 0.0 24-25 0.0 0.0 0.0 0.00938559137916051 0.0 26-27 0.0 0.0 0.0 0.01651168853741201 0.0 28-29 0.0 0.0 0.0 0.047275571391327015 0.0 30-31 0.0 0.0 0.0 0.11888415746936647 0.0 32-33 0.0 0.0 0.0 0.2532371599895716 0.0 34-35 0.0 0.0 0.0 0.47814373859389936 0.0 36-37 0.0 0.0 0.0 0.7981228817241679 0.0 38-39 0.0 0.0 0.0 1.1448683410098202 0.0 40-41 0.0 0.0 0.0 1.4566785434952636 0.0 42-43 0.0 0.0 0.0 1.7743981924046233 0.0 44-45 0.0 0.0 0.0 2.1637264273920223 0.0 46-47 0.0 0.0 0.0 2.5900756061527765 0.0 48-49 0.0 0.0 0.0 2.9913965412357695 0.0 50-51 0.0 0.0 0.0 3.366472581906666 0.0 52-53 0.0 0.0 0.0 3.7422438515686105 0.0 54-55 0.0 0.0 0.0 4.204918745111671 0.0 56-57 0.0 0.0 0.0 4.747023550882072 0.0 58-59 0.0 0.0 0.0 5.243069435995481 0.0 60-61 0.0 0.0 0.0 5.752672286434345 0.0 62-63 0.0 0.0 0.0 6.245589641088033 0.0 64-65 0.0 0.0 0.0 6.8043799426436085 0.0 66-67 0.0 0.0 0.0 7.457547579734075 0.0 68-69 0.0 0.0 0.0 8.187190405839925 0.0 70-71 0.0 0.0 0.0 8.90414530285913 0.0 72-73 0.0 0.0 0.0 9.589814895281133 0.0 74-75 0.0 0.0 0.0 10.335969409924395 0.0 76-77 0.0 0.0 0.0 11.138958894585905 0.0 78-79 0.0 0.0 0.0 12.087598852872166 0.0 80-81 0.0 0.0 0.0 12.98600851655514 0.0 82-83 0.0 0.0 0.0 13.870861214912662 0.0 84-85 0.0 0.0 0.0 14.754323455288086 0.0 86-87 0.0 0.0 0.0 15.65029981750239 0.0 88-89 0.0 0.0 0.0 16.63317980359781 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ATTCCGT 25 0.0050175316 56.999794 6 GTAAGCG 35 4.3065447E-6 40.714134 94-95 TCGTATC 25 0.0016151739 37.999863 94-95 CCTGTAC 85 1.62801E-5 33.52929 3 AAGCGCA 30 0.003949397 31.666552 68-69 GATAGTA 80 4.4854562E-4 29.687391 7 GTCCAAG 130 3.019013E-7 29.235743 1 CATTCCG 65 0.0058369907 29.230661 9 CGGTAAA 35 0.008388299 27.142757 14-15 TTATACT 105 0.002192296 22.618963 4 CTGTACT 150 3.0231098E-5 22.166586 4 CTCCGAC 140 6.548362E-11 22.05349 94-95 GTATTAA 110 0.002869345 21.594585 1 ATAGCCC 135 3.7864994E-4 21.111032 3 GTACTTT 1430 0.0 21.092579 14-15 GTGTAGC 160 4.966103E-5 20.784788 1 AGTACTT 1430 0.0 20.760414 12-13 TCCAAGG 185 6.5144322E-6 20.544035 2 TTAGGCA 140 4.8397048E-4 20.357067 4 TAATAGT 140 4.8397048E-4 20.357067 4 >>END_MODULE