FastQCFastQC Report
Thu 26 May 2016
SRR938455_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938455_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012478
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67750.2248979079681246No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54910.1822751900594793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35630.11827472267017386No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50500.046.893511
GTATCAA89900.042.5276221
ATCAACG111500.033.5657463
TATCAAC114800.032.8082
TCAACGC113550.032.7922364
CAACGCA114700.032.380545
AACGCAG118800.031.5206976
ACGCAGA135150.027.4293237
CGCAGAG135950.027.2679168
TACATGG111800.026.37222
GTACATG114200.025.858321
ACATGGG115550.024.446293
GCAGAGT149800.024.1106829
GAGTACT76100.021.22954412-13
AGAGTAC130400.021.2100810-11
CATGGGG66500.020.3092164
AGTACTT79600.020.17669312-13
CAGAGTA145050.019.8541710-11
ACGTAAC2651.834269E-819.7388381
GTACTTT81150.019.66946214-15
Produced by FastQC (version 0.11.5)