##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938455_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3012478 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.94881622372014 31.0 31.0 34.0 27.0 34.0 2 30.99712197068327 31.0 31.0 34.0 27.0 34.0 3 31.03512423991146 31.0 31.0 34.0 27.0 34.0 4 34.68873266460369 37.0 35.0 37.0 32.0 37.0 5 34.65997394835747 37.0 35.0 37.0 32.0 37.0 6 34.665288178038146 37.0 35.0 37.0 30.0 37.0 7 34.567606136874694 37.0 35.0 37.0 30.0 37.0 8 34.53412672225324 37.0 35.0 37.0 30.0 37.0 9 36.00098490345822 39.0 35.0 39.0 30.0 39.0 10-11 35.82921701004953 38.0 35.0 39.0 30.0 39.0 12-13 35.68587986368697 38.0 35.0 39.0 30.0 39.0 14-15 36.620353244073485 39.0 36.0 41.0 30.0 41.0 16-17 36.43929084295387 39.0 36.0 40.5 29.5 41.0 18-19 36.58365720845099 39.0 36.0 41.0 30.0 41.0 20-21 36.56010467130382 39.0 36.0 41.0 30.0 41.0 22-23 36.46674382352336 39.0 36.0 41.0 29.0 41.0 24-25 36.323978797521505 39.0 35.5 41.0 29.0 41.0 26-27 36.119355726415264 39.0 35.0 40.5 27.5 41.0 28-29 35.90723749683815 39.0 35.0 40.0 27.0 41.0 30-31 35.660040836812755 38.0 34.5 40.0 27.0 41.0 32-33 35.39748257082707 38.0 34.0 40.0 26.5 41.0 34-35 35.149638271217256 38.0 34.0 40.0 25.5 41.0 36-37 34.87625171038593 38.0 34.0 40.0 25.0 41.0 38-39 34.751354034784654 38.0 33.5 40.0 24.5 41.0 40-41 34.65684346906434 38.0 33.0 40.0 24.0 41.0 42-43 34.37205118178456 38.0 33.0 40.0 24.0 41.0 44-45 34.04542738569377 38.0 33.0 40.0 23.0 41.0 46-47 33.68042588194835 38.0 33.0 40.0 21.0 41.0 48-49 33.31920614855943 37.5 32.0 40.0 18.5 41.0 50-51 32.632978564490756 36.5 30.5 39.5 17.5 40.5 52-53 32.676420209541774 37.0 31.0 39.0 17.5 40.0 54-55 32.78685354714624 37.0 31.0 40.0 15.5 41.0 56-57 32.468612218910806 37.0 31.0 40.0 12.5 41.0 58-59 32.09131187016138 36.0 31.0 39.0 8.5 41.0 60-61 31.613881495566112 36.0 30.0 39.0 7.5 40.5 62-63 31.04283500161661 35.0 29.0 39.0 4.5 40.0 64-65 30.521106046251624 35.0 28.5 38.0 2.0 40.0 66-67 29.980433052125193 34.5 28.0 38.0 2.0 40.0 68-69 29.397643567853443 34.0 27.5 37.5 2.0 40.0 70-71 28.758200391836887 34.0 26.0 37.0 2.0 39.0 72-73 28.11933763499684 33.5 26.0 36.0 2.0 39.0 74-75 27.43794245136396 33.0 25.0 36.0 2.0 38.5 76-77 26.69081516943858 32.5 23.0 35.0 2.0 37.5 78-79 25.91118258788944 32.0 20.5 35.0 2.0 37.0 80-81 25.216709134473348 31.0 19.0 35.0 2.0 36.0 82-83 24.485241054042554 31.0 15.0 34.0 2.0 36.0 84-85 23.781081720762774 30.5 7.5 34.0 2.0 35.0 86-87 23.02818194854867 30.0 3.5 34.0 2.0 35.0 88-89 22.215069122496494 29.0 2.0 34.0 2.0 35.0 90-91 21.311526756377972 29.0 2.0 34.0 2.0 35.0 92-93 20.368348084201777 27.5 2.0 33.5 2.0 35.0 94-95 19.369376971383694 25.5 2.0 33.0 2.0 35.0 96-97 18.13307881418553 24.5 2.0 32.5 2.0 34.0 98-99 16.641762529054155 11.0 2.0 32.0 2.0 34.0 100-101 13.230098443872453 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5269252946367011 1101 2 0.43579409376784994 1101 3 0.49111099242172074 1101 4 0.3537328889330169 1101 5 0.38724354965067675 1101 6 0.5270064569982651 1101 7 0.4822037379650439 1101 8 0.5136653228445809 1101 9 0.7151695691265445 1101 10-11 0.7367677240006998 1101 12-13 0.7558549186638359 1101 14-15 0.8899604349143857 1101 16-17 0.7441881633486958 1101 18-19 0.9086110834150247 1101 20-21 0.8958762640648175 1101 22-23 0.8516043722928117 1101 24-25 0.8993997352346881 1101 26-27 0.7848333241884475 1101 28-29 0.7980780973099044 1101 30-31 0.9539934780658399 1101 32-33 0.8439185747147064 1101 34-35 0.7996421297734173 1101 36-37 0.96771030295222 1101 38-39 1.0452242753190362 1101 40-41 1.059964357909628 1101 42-43 1.2044805755739176 1101 44-45 1.1018453433118012 1101 46-47 1.0115317892740237 1101 48-49 0.9096111825757376 1101 50-51 1.0191142298269469 1101 52-53 1.0982004439425879 1101 54-55 1.1607061112055277 1101 56-57 1.1274189049152952 1101 58-59 1.1701026834659487 1101 60-61 1.3413361649209072 1101 62-63 1.2013466631449425 1101 64-65 1.0755395889104413 1101 66-67 1.1305642569414402 1101 68-69 1.1740135539210108 1101 70-71 1.1097949458518812 1101 72-73 1.310456668553634 1101 74-75 1.2856493900477695 1101 76-77 1.3871036982184002 1101 78-79 1.5947555654832044 1101 80-81 1.510263979975985 1101 82-83 1.5778300489481047 1101 84-85 1.515101214700291 1101 86-87 1.3126656272018629 1101 88-89 1.4162028047628965 1101 90-91 1.0359618231656142 1101 92-93 1.1330891451833516 1101 94-95 1.1593651078307161 1101 96-97 1.1196588418178273 1101 98-99 1.299243158537763 1101 100-101 1.2988613799557047 1102 1 0.5030241753231941 1102 2 0.5206609344003006 1102 3 0.49016582693016275 1102 4 0.3751251635501234 1102 5 0.44241589232169076 1102 6 0.5709513717544468 1102 7 0.5391457654127336 1102 8 0.5425811495643202 1102 9 0.8366207876780791 1102 10-11 0.8710488154972467 1102 12-13 0.8403627405388434 1102 14-15 0.997371653098142 1102 16-17 1.0086352048825589 1102 18-19 0.9166634411351282 1102 20-21 0.9773158081389539 1102 22-23 0.9211606999067712 1102 24-25 0.8635683089231705 1102 26-27 0.8741277936222147 1102 28-29 0.5816098528575822 1102 30-31 0.7402947536354461 1102 32-33 0.882924698472614 1102 34-35 0.6937604843231711 1102 36-37 1.0039335498733806 1102 38-39 0.9492404023993686 1102 40-41 1.1069995426176078 1102 42-43 1.052892344679698 1102 44-45 1.0538178404497174 1102 46-47 1.082678820346075 1102 48-49 1.3318305360164402 1102 50-51 1.1776818002757565 1102 52-53 1.041286248369488 1102 54-55 0.8939438056030937 1102 56-57 1.028697133456241 1102 58-59 1.1299668837977919 1102 60-61 1.0029330609710918 1102 62-63 1.0497939054658865 1102 64-65 1.0748056474672154 1102 66-67 1.0804584336932272 1102 68-69 0.9296487009068564 1102 70-71 1.0588790783783217 1102 72-73 1.2486779210227823 1102 74-75 1.2005602671668143 1102 76-77 1.3070968123144233 1102 78-79 1.3066027868805534 1102 80-81 1.563461191420945 1102 82-83 1.6774884748120655 1102 84-85 1.562151557839968 1102 86-87 1.6404193737573571 1102 88-89 1.6164967540885087 1102 90-91 1.5603963453258736 1102 92-93 1.666596796339629 1102 94-95 1.6929934673181428 1102 96-97 1.7938959440425641 1102 98-99 1.922508232541972 1102 100-101 1.800532845549915 1103 1 0.48509630170698514 1103 2 0.4377935005643998 1103 3 0.32721655991515775 1103 4 0.27279272320649284 1103 5 0.37066509380223067 1103 6 0.5454704700220674 1103 7 0.3945634849867403 1103 8 0.4517575538797871 1103 9 0.5443024029868511 1103 10-11 0.584585725626205 1103 12-13 0.5901710590579583 1103 14-15 0.7641269062750382 1103 16-17 0.8352681072684973 1103 18-19 0.7543983140316399 1103 20-21 0.7746445692193404 1103 22-23 0.923287156130904 1103 24-25 0.8554295791470992 1103 26-27 0.8672201950797671 1103 28-29 1.044580802411474 1103 30-31 0.9963731969288929 1103 32-33 0.9472110514280132 1103 34-35 0.9319634198254505 1103 36-37 0.9394589886805917 1103 38-39 1.0004843994768606 1103 40-41 0.9708812894227918 1103 42-43 0.9677835704116688 1103 44-45 1.0357904598915937 1103 46-47 0.8286803452484008 1103 48-49 0.8809562701621516 1103 50-51 1.084185647620238 1103 52-53 1.0772540893407765 1103 54-55 1.1370914524123492 1103 56-57 1.1845285667967431 1103 58-59 1.059168822686992 1103 60-61 1.098909881315823 1103 62-63 1.2344409698982979 1103 64-65 1.438577259799093 1103 66-67 1.2628247942671962 1103 68-69 1.4477168104400135 1103 70-71 1.5514804166398228 1103 72-73 1.5390517049571102 1103 74-75 1.5507342796346286 1103 76-77 1.5689138256978659 1103 78-79 1.6039479345456478 1103 80-81 1.6689972090747638 1103 82-83 1.5139521999601193 1103 84-85 1.622861301196366 1103 86-87 1.888173610134789 1103 88-89 1.682261343225175 1103 90-91 1.587134264345984 1103 92-93 1.5628691808002557 1103 94-95 1.5047264519576196 1103 96-97 1.8114144498995195 1103 98-99 1.814363363733797 1103 100-101 1.5760293323492771 1104 1 0.41243299247812004 1104 2 0.39258309234005395 1104 3 0.3266551126714816 1104 4 0.3548925860474199 1104 5 0.4180139253286157 1104 6 0.45779123944414124 1104 7 0.39427808833668365 1104 8 0.5122802218278508 1104 9 0.5873102192534105 1104 10-11 0.7055492835285619 1104 12-13 0.6230202248768322 1104 14-15 0.7122756120627685 1104 16-17 0.6965680707732886 1104 18-19 0.7539172200780087 1104 20-21 0.727083684790621 1104 22-23 0.6624421974051415 1104 24-25 0.5818973488155876 1104 26-27 0.7542322995905621 1104 28-29 0.7564277495677345 1104 30-31 0.7761508724825958 1104 32-33 0.7964328896514203 1104 34-35 0.6382041578265429 1104 36-37 0.7975828316786959 1104 38-39 0.8968593719230853 1104 40-41 0.8642975511506634 1104 42-43 0.8530757725864717 1104 44-45 0.8045034874052419 1104 46-47 0.6526829329662647 1104 48-49 0.5535386106289621 1104 50-51 0.6854005323776633 1104 52-53 0.6741106344410852 1104 54-55 0.8534225120705017 1104 56-57 0.9718634977738319 1104 58-59 0.8812429314436301 1104 60-61 0.9366315680647297 1104 62-63 0.9175208123465772 1104 64-65 0.8697189922889734 1104 66-67 0.9073511851370597 1104 68-69 1.1063822460106927 1104 70-71 1.1908550788425707 1104 72-73 1.161974173492247 1104 74-75 0.8975049435944378 1104 76-77 0.7692745834761219 1104 78-79 0.8971649543070868 1104 80-81 1.0237586373071927 1104 82-83 1.1032783880433463 1104 84-85 1.0670242558449665 1104 86-87 1.1192721798006353 1104 88-89 1.168132214462279 1104 90-91 1.1348991093598713 1104 92-93 1.0411713419960194 1104 94-95 0.9335287639440288 1104 96-97 0.9853554448259807 1104 98-99 1.2246634473696076 1104 100-101 1.27368412184987 1105 1 0.3996246282255207 1105 2 0.3008458299148984 1105 3 0.24432789261669186 1105 4 0.2916488131484414 1105 5 0.31893780240206127 1105 6 0.45023140410589235 1105 7 0.37282330361824734 1105 8 0.3974051511460317 1105 9 0.5251689537195503 1105 10-11 0.5683527420106813 1105 12-13 0.6640436546644963 1105 14-15 0.7867809253564673 1105 16-17 0.7356441463634624 1105 18-19 0.7710899987322293 1105 20-21 0.6335899198527741 1105 22-23 0.7737027050060803 1105 24-25 0.813837766081491 1105 26-27 0.7973312145546245 1105 28-29 0.6909521738497517 1105 30-31 0.6866733843747923 1105 32-33 0.8457665440114894 1105 34-35 0.9099942777769314 1105 36-37 1.004601504590319 1105 38-39 0.7502062047318532 1105 40-41 0.8827798262576678 1105 42-43 0.9173709510747514 1105 44-45 0.978370340491189 1105 46-47 0.9385224910338081 1105 48-49 0.923602716999838 1105 50-51 1.0227431496664607 1105 52-53 1.005134705421554 1105 54-55 0.9469203651504259 1105 56-57 1.0381443298957933 1105 58-59 1.0276233765261154 1105 60-61 1.0925570717883808 1105 62-63 1.1798099825570212 1105 64-65 1.1353579645903054 1105 66-67 1.1398938502786322 1105 68-69 1.1357642609397267 1105 70-71 1.1800661491870166 1105 72-73 1.1496113091419637 1105 74-75 1.3211035930444304 1105 76-77 1.3573836198192772 1105 78-79 1.1604037568859695 1105 80-81 1.248522643927398 1105 82-83 1.3108860761602834 1105 84-85 1.47111138663195 1105 86-87 1.5838118507008971 1105 88-89 1.6748657071901185 1105 90-91 1.631431611810605 1105 92-93 1.9168281342934463 1105 94-95 1.7735409380007638 1105 96-97 1.9044271224359584 1105 98-99 1.795758066772187 1105 100-101 1.6137862350320216 1106 1 0.31284850598959224 1106 2 0.10105240357596657 1106 3 0.16415232082206188 1106 4 0.10488249762536839 1106 5 0.2083108054706102 1106 6 0.025191070044968455 1106 7 0.23540626524552266 1106 8 0.23720585343320977 1106 9 0.18652386182115777 1106 10-11 0.14167721766788333 1106 12-13 0.26699231177752125 1106 14-15 0.5118516285926802 1106 16-17 0.24389744426608928 1106 18-19 0.2218596675184088 1106 20-21 0.329084051642333 1106 22-23 0.18478492322125106 1106 24-25 0.13486161853791856 1106 26-27 0.11736791064142693 1106 28-29 0.37481554419551344 1106 30-31 0.1276637042001667 1106 32-33 0.20774923990848748 1106 34-35 -0.0700084111604582 1106 36-37 0.04634093073944712 1106 38-39 0.18431126309761225 1106 40-41 0.47305694498216155 1106 42-43 0.16612091153155717 1106 44-45 0.13416215346768467 1106 46-47 0.2910094417788045 1106 48-49 0.5810249750745555 1106 50-51 0.29386158945008134 1106 52-53 0.16784151634180944 1106 54-55 0.2967822803065445 1106 56-57 -0.28778335350556006 1106 58-59 -0.033067522211666756 1106 60-61 0.6033253170194719 1106 62-63 0.6125470044797687 1106 64-65 0.5700166277139012 1106 66-67 0.25561764895631 1106 68-69 0.31220553138356166 1106 70-71 0.11058954294235335 1106 72-73 0.5630159784026638 1106 74-75 0.9476585897228027 1106 76-77 0.7253113981242976 1106 78-79 0.9506146012123153 1106 80-81 1.033256913643637 1106 82-83 0.9162446538705744 1106 84-85 0.847881340129149 1106 86-87 0.8063758315936234 1106 88-89 0.8413063449427973 1106 90-91 0.9934551162868956 1106 92-93 0.9018615087517006 1106 94-95 1.0344344560799144 1106 96-97 1.0209300802670889 1106 98-99 1.0630797269537666 1106 100-101 0.6430005907942551 1107 1 0.22629218520017602 1107 2 0.12723304104536837 1107 3 0.30140280596341995 1107 4 0.24207616098165374 1107 5 0.16711044526108054 1107 6 0.3814459056042452 1107 7 0.30558269181478437 1107 8 0.1865593787937314 1107 9 0.5168268690634008 1107 10-11 0.49153858773244963 1107 12-13 0.5243445180609001 1107 14-15 0.6047846727437118 1107 16-17 0.7548209251077651 1107 18-19 0.677516605991066 1107 20-21 0.7610130751447386 1107 22-23 0.6916636569071883 1107 24-25 0.6241532487397095 1107 26-27 0.6428623743224122 1107 28-29 0.7719726792614026 1107 30-31 0.7899677768508795 1107 32-33 0.9272984706582079 1107 34-35 0.8673699229972982 1107 36-37 0.8629410093767333 1107 38-39 0.951986770117891 1107 40-41 0.7868755465067139 1107 42-43 0.827870464440501 1107 44-45 0.8575265612147192 1107 46-47 0.8971606536809347 1107 48-49 0.8436101126263935 1107 50-51 0.8113583868829508 1107 52-53 0.983202423461023 1107 54-55 1.0327108979390474 1107 56-57 0.9784127577535244 1107 58-59 0.8340912617006566 1107 60-61 0.8471039657263937 1107 62-63 1.0249260362264074 1107 64-65 1.0235145920227176 1107 66-67 1.0059041677341334 1107 68-69 1.0501901336944819 1107 70-71 1.2551401054526572 1107 72-73 1.1824231546897153 1107 74-75 1.178515609369441 1107 76-77 1.2143006552555597 1107 78-79 1.1958622452644185 1107 80-81 1.2157438814150332 1107 82-83 1.0393383971324965 1107 84-85 1.1869310972413878 1107 86-87 1.3237843523246973 1107 88-89 1.323248432382396 1107 90-91 1.376503706523188 1107 92-93 1.4911462898990457 1107 94-95 1.761911340521145 1107 96-97 2.0562788112895056 1107 98-99 2.0583267368341644 1107 100-101 2.0168047332038537 1108 1 0.3183253665274286 1108 2 0.17406991452030596 1108 3 0.24277646090961724 1108 4 0.22986217242211637 1108 5 0.24705064286898448 1108 6 0.30952502876917265 1108 7 0.31392252540813104 1108 8 0.3712677671292681 1108 9 0.29194287370358296 1108 10-11 0.4182274052851511 1108 12-13 0.3397588846355646 1108 14-15 0.5063739863162553 1108 16-17 0.5662481321960513 1108 18-19 0.6537929070431119 1108 20-21 0.4628919002977483 1108 22-23 0.4205580626834191 1108 24-25 0.5173334759487958 1108 26-27 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-3.485926477908402 2316 100-101 -3.1245411574337254 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 12565.0 3 4569.0 4 4834.0 5 5311.0 6 6281.0 7 7710.0 8 9547.0 9 12212.0 10 14806.0 11 18157.0 12 21486.0 13 24387.0 14 27396.0 15 30781.0 16 35010.0 17 40115.0 18 39208.0 19 38656.0 20 41435.0 21 45256.0 22 49497.0 23 54999.0 24 60900.0 25 67924.0 26 76120.0 27 85949.0 28 96695.0 29 109628.0 30 125211.0 31 145228.0 32 168618.0 33 200558.0 34 241505.0 35 289251.0 36 335162.0 37 317569.0 38 141148.0 39 6794.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.74297874035614 18.286547067337697 14.188322466702964 23.7821517256032 2 15.584994302731797 19.61255917831735 40.26754065762658 24.534905861324273 3 19.630215516525148 23.575713264983968 28.985693158970438 27.808378059520443 4 13.050182836226398 15.940700111699094 36.32217860089991 34.686938451174605 5 14.21047697066576 37.12946536623251 33.68683456700809 14.973223096093644 6 33.211411072951336 35.197510074535614 17.149836787206667 14.441242065306383 7 29.57878218784366 31.947015900675236 19.954105593658856 18.52009631782225 8 26.689633188671912 34.92062437948885 19.37249927779973 19.017243154039505 9 26.639050743594588 16.700937549077974 19.580388931628345 37.07962277569909 10-11 25.43534808583677 26.225354741600913 26.981783665080883 21.357513507481425 12-13 26.18012986057566 23.41196963177607 27.38457598856401 23.023324519084262 14-15 22.924845341894773 25.051973291655088 25.498407347750046 26.524774018700093 16-17 22.801257233233503 28.21127508626419 26.17790902873901 22.809558651763297 18-19 22.732346664492688 26.791484365050756 28.32649565639125 22.149673314065303 20-21 23.348725005555153 26.072924773118977 28.37938379503368 22.19896642629219 22-23 23.016935341139156 25.98959393500875 28.209806831317014 22.78366389253508 24-25 22.805547787746406 26.173662455222548 28.352604880368727 22.66818487666232 26-27 22.379670863015143 26.51016647831398 28.18956390470489 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23.132192255379074 26.689896789070776 27.275751357571142 22.902159597979004 54-55 22.78184909927407 26.782561552869964 27.38816370895507 23.047425638900894 56-57 22.793217707743516 26.955682501233795 27.245202975581904 23.00589681544078 58-59 22.808363970435654 26.6390393214052 27.294927305983556 23.25766940217559 60-61 22.636013292742994 26.734736330769294 27.563631860016137 23.065618516471574 62-63 22.60038577352239 26.76642442391265 27.62221418066285 23.01097562190211 64-65 22.446281381335673 26.67201270300566 27.578592460893624 23.303113454765047 66-67 22.428219141517996 26.766443548371033 27.403114183404963 23.402223126706005 68-69 22.839721864547986 26.42993935021718 27.565962884397898 23.164375900836934 70-71 22.678341302116557 26.42537396942365 27.36032658341588 23.53595814504392 72-73 22.695582631759915 26.527270092028438 27.32740330921179 23.449743966999858 74-75 22.53176565437613 26.813396662091005 27.24325322272052 23.411584460812342 76-77 22.67203648082492 26.454757229460114 27.328278947889185 23.54492734182578 78-79 22.820357433744494 26.593328197781492 27.257597056369402 23.328717312104615 80-81 22.770874417790946 26.681553203712127 27.078586541399773 23.468985837097158 82-83 22.778045661160625 26.65578350079964 27.16446499723218 23.40170584080756 84-85 22.70454943817539 26.599809184126034 27.10348939424923 23.592151983449344 86-87 22.733708238388353 26.58004021511584 27.0306467203499 23.6556048261459 88-89 22.733936917245643 26.52219306582505 26.99754528373691 23.7463247331924 90-91 22.6349544588215 26.524031798546556 27.08377229095282 23.757241451679132 92-93 22.75244163121303 26.205931306423157 27.18030922486251 23.861317837501296 94-95 22.590633085308458 26.106758908960664 27.26545558525906 24.037152420471823 96-97 22.70394599852483 25.923999153819256 27.25445897069485 24.11759587696107 98-99 22.58894457211409 25.931266247166445 27.00007205283166 24.4797171278878 100-101 23.189906713371712 25.394632352220597 27.188410486411623 24.227050447996064 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 325.0 1 251.5 2 213.0 3 268.5 4 263.5 5 257.0 6 428.5 7 940.0 8 1547.0 9 1828.5 10 1695.5 11 1500.0 12 1581.5 13 1755.5 14 1908.5 15 2083.5 16 2289.0 17 2507.0 18 2766.0 19 3081.5 20 3488.0 21 4033.0 22 4750.0 23 5989.0 24 7567.0 25 9460.0 26 12365.5 27 16267.0 28 20089.5 29 24143.0 30 28808.0 31 35012.5 32 43055.5 33 48238.0 34 51808.0 35 57250.0 36 63479.5 37 69705.5 38 74909.5 39 79121.0 40 84014.5 41 89846.5 42 94755.5 43 99937.0 44 105138.5 45 110472.5 46 114633.5 47 118590.5 48 122007.5 49 121984.0 50 119428.5 51 115218.5 52 111793.0 53 109947.0 54 109286.0 55 104159.0 56 94984.0 57 86538.0 58 78902.0 59 69061.5 60 57079.5 61 47735.5 62 39734.5 63 30488.5 64 22284.0 65 16843.5 66 12647.0 67 9527.5 68 7387.0 69 5540.0 70 4101.5 71 3016.5 72 2217.0 73 1771.5 74 1409.0 75 1065.5 76 759.5 77 483.0 78 293.0 79 187.0 80 116.5 81 69.0 82 48.0 83 32.5 84 20.5 85 15.5 86 10.0 87 5.5 88 4.0 89 3.5 90 3.5 91 3.0 92 2.5 93 3.0 94 2.5 95 1.0 96 0.0 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.006572662107407921 2 0.10426632161297113 3 0.11379336214239573 4 0.11621661635371279 5 0.1547563168926047 6 0.0965982158209952 7 0.025958695797944416 8 0.028149583167080387 9 0.022871536323252818 10-11 0.05158543896420156 12-13 0.05422446238611535 14-15 0.02771804474588694 16-17 0.03135292606286254 18-19 0.0364981918540152 20-21 0.05839046791379057 22-23 0.032033428957821436 24-25 0.04899620843704087 26-27 0.057278426597638224 28-29 0.05404188843868735 30-31 0.067817922653709 32-33 0.050158042647946315 34-35 0.06225771607294725 36-37 0.053228604491053544 38-39 0.031983636063068345 40-41 0.03722848764372719 42-43 0.03326165369506433 44-45 0.007568520002469728 46-47 0.01365985079393111 48-49 0.009195087897737344 50-51 0.008315413423766082 52-53 0.00985899316111188 54-55 0.011684732635391859 56-57 0.014224170267799466 58-59 0.02014952474341721 60-61 0.025211802376648062 62-63 0.011551951582716952 64-65 0.009211685529321709 66-67 0.010755265266667507 68-69 0.011568549214301317 70-71 0.031037571062759627 72-73 0.02836535237767712 74-75 0.029610174746504375 76-77 0.023369465270783717 78-79 0.004763520264712307 80-81 0.004531153422531219 82-83 0.0059917450019552005 84-85 0.004979289475309031 86-87 0.01415777974146201 88-89 0.037577037906998824 90-91 0.03593387238014684 92-93 0.030390263430969455 94-95 0.04722026185751398 96-97 0.043187037382513664 98-99 0.026523015271812774 100-101 0.07434079186636385 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3012478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.859793164423834 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98242770755547 41.68380268167279 2 13.020397201918247 14.285925948317555 3 4.6426975399182275 7.6409228029467995 4 2.1345098229180484 4.683950695708602 5 1.1402688061339812 3.127745542817735 6 0.7019618315014821 2.310568853129486 7 0.46015530008364597 1.7670817210271539 8 0.32494943058624803 1.426132684068709 9 0.22864197780043763 1.1288926449753114 >10 1.2381698020499075 12.223173302668865 >50 0.08088405200484135 3.0368815901051516 >100 0.040622639349738636 3.917177706483738 >500 0.002734154480308917 1.0468661450685535 >1k 0.0014582157224670989 1.3120242364587265 >5k 1.2151797687225825E-4 0.4088534445506514 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6775 0.2248979079681246 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5491 0.1822751900594793 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3563 0.11827472267017386 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.979289475309032E-5 14-15 0.0 0.0 0.0 1.659763158436344E-5 1.1618342109054406E-4 16-17 0.0 0.0 0.0 3.319526316872688E-5 1.6597631584363437E-4 18-19 0.0 0.0 0.0 3.319526316872688E-5 1.6597631584363437E-4 20-21 0.0 0.0 0.0 9.958578950618062E-5 1.6597631584363437E-4 22-23 0.0 0.0 0.0 2.489644737654516E-4 1.6597631584363437E-4 24-25 0.0 0.0 0.0 5.311242106996301E-4 1.9917157901236124E-4 26-27 0.0 0.0 0.0 0.0010124555266461696 2.6556210534981503E-4 28-29 0.0 0.0 0.0 0.0034523073695475947 2.6556210534981503E-4 30-31 0.0 0.0 0.0 0.012000087635494764 2.6556210534981503E-4 32-33 0.0 0.0 0.0 0.03141931658919999 2.8215973693417843E-4 34-35 0.0 0.0 0.0 0.06036558607232982 3.3195263168726873E-4 36-37 0.0 0.0 0.0 0.10984312582531723 3.3195263168726873E-4 38-39 0.0 0.0 0.0 0.18614243821863596 3.3195263168726873E-4 40-41 0.0 0.0 0.0 0.26971151324590587 3.3195263168726873E-4 42-43 3.319526316872688E-5 0.0 0.0 0.354160262747147 3.3195263168726873E-4 44-45 3.319526316872688E-5 0.0 0.0 0.4590738919919083 3.3195263168726873E-4 46-47 3.319526316872688E-5 0.0 0.0 0.5865768978229883 3.485502632716322E-4 48-49 3.319526316872688E-5 0.0 0.0 0.722295731288328 3.6514789485599564E-4 50-51 3.319526316872688E-5 0.0 0.0 0.8710769008105619 3.6514789485599564E-4 52-53 3.319526316872688E-5 0.0 0.0 1.0148455855943181 3.983431580247225E-4 54-55 6.639052633745376E-5 0.0 0.0 1.1763571385417586 4.1494078960908594E-4 56-57 6.639052633745376E-5 0.0 0.0 1.362881322286835 4.315384211934494E-4 58-59 6.639052633745376E-5 0.0 0.0 1.568160829722242 4.315384211934494E-4 60-61 6.639052633745376E-5 0.0 0.0 1.7889923179521974 4.315384211934494E-4 62-63 6.639052633745376E-5 0.0 0.0 2.0082470311816385 4.6473368436217625E-4 64-65 6.639052633745376E-5 0.0 0.0 2.238489376519928 4.6473368436217625E-4 66-67 6.639052633745376E-5 0.0 0.0 2.503155209764188 4.6473368436217625E-4 68-69 6.639052633745376E-5 0.0 0.0 2.7974810106497046 4.6473368436217625E-4 70-71 6.639052633745376E-5 0.0 0.0 3.106080774697774 4.6473368436217625E-4 72-73 6.639052633745376E-5 0.0 0.0 3.425452401644095 4.6473368436217625E-4 74-75 6.639052633745376E-5 0.0 0.0 3.74955435359196 4.6473368436217625E-4 76-77 6.639052633745376E-5 0.0 0.0 4.101241569233037 4.813313159465397E-4 78-79 6.639052633745376E-5 0.0 0.0 4.494074313571751 5.145265791152666E-4 80-81 9.958578950618062E-5 0.0 1.659763158436344E-5 4.908284807391125 5.311242106996301E-4 82-83 9.958578950618062E-5 0.0 3.319526316872688E-5 5.33965725226873 5.311242106996301E-4 84-85 9.958578950618062E-5 0.0 3.319526316872688E-5 5.779959222938723 5.311242106996301E-4 86-87 9.958578950618062E-5 0.0 3.319526316872688E-5 6.228244654400796 5.477218422839935E-4 88-89 9.958578950618062E-5 0.0 3.319526316872688E-5 6.708214964557418 5.643194738683569E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5050 0.0 46.89351 1 GTATCAA 8990 0.0 42.527622 1 ATCAACG 11150 0.0 33.565746 3 TATCAAC 11480 0.0 32.808 2 TCAACGC 11355 0.0 32.792236 4 CAACGCA 11470 0.0 32.38054 5 AACGCAG 11880 0.0 31.520697 6 ACGCAGA 13515 0.0 27.429323 7 CGCAGAG 13595 0.0 27.267916 8 TACATGG 11180 0.0 26.3722 2 GTACATG 11420 0.0 25.85832 1 ACATGGG 11555 0.0 24.44629 3 GCAGAGT 14980 0.0 24.110682 9 GAGTACT 7610 0.0 21.229544 12-13 AGAGTAC 13040 0.0 21.21008 10-11 CATGGGG 6650 0.0 20.309216 4 AGTACTT 7960 0.0 20.176693 12-13 CAGAGTA 14505 0.0 19.85417 10-11 ACGTAAC 265 1.834269E-8 19.738838 1 GTACTTT 8115 0.0 19.669462 14-15 >>END_MODULE