FastQCFastQC Report
Thu 26 May 2016
SRR938455_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938455_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3012478
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69330.23014275954878344No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57090.18951175743026175No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39630.1315528279376646No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA54450.046.8677641
GTATCAA88900.037.632991
ATCAACG113750.028.9381333
TATCAAC115000.028.8853192
TCAACGC115250.028.5614994
CAACGCA117800.027.9029085
AACGCAG122750.026.922656
TACATGG112750.024.994012
GTACATG118350.024.0924421
ACGCAGA141600.023.2380717
ACATGGG116200.023.0548153
CGCAGAG143050.022.9693288
TAAGGTG15000.021.2164255
GTAAGGT14700.020.356914
GAGTACT81550.020.11658112-13
GCAGAGT159350.020.0238349
CATGGGG63000.019.3013694
GTACTTT84500.019.18951814-15
AGAGTAC139400.018.9894410-11
TGGTATC27150.018.9039152
Produced by FastQC (version 0.11.5)