##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938455_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3012478 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.783064639808156 31.0 31.0 34.0 30.0 34.0 2 32.00856603766069 31.0 31.0 34.0 30.0 34.0 3 32.07316401978703 33.0 31.0 34.0 30.0 34.0 4 35.751467396608376 37.0 35.0 37.0 35.0 37.0 5 35.64564587691595 37.0 35.0 37.0 33.0 37.0 6 35.64006475731939 37.0 35.0 37.0 33.0 37.0 7 35.57584287752475 37.0 35.0 37.0 33.0 37.0 8 35.54686341277845 37.0 35.0 37.0 33.0 37.0 9 37.12984991093711 39.0 37.0 39.0 33.0 39.0 10-11 37.03001930636506 39.0 37.0 39.0 33.0 39.0 12-13 36.92186465760082 39.0 37.0 39.0 33.0 39.0 14-15 37.97470603934701 40.0 37.0 41.0 33.0 41.0 16-17 37.88562870832584 40.0 37.0 41.0 33.0 41.0 18-19 38.11310953972112 40.0 37.0 41.0 33.0 41.0 20-21 38.077173509648865 40.0 37.0 41.0 33.0 41.0 22-23 37.970004096295476 40.0 37.0 41.0 33.0 41.0 24-25 37.85237684723341 40.0 37.0 41.0 32.0 41.0 26-27 37.67292972761959 40.0 37.0 41.0 32.0 41.0 28-29 37.55930898084567 39.5 36.5 41.0 32.0 41.0 30-31 37.319142247677824 39.0 36.0 41.0 31.0 41.0 32-33 37.145666126026484 39.0 36.0 41.0 31.0 41.0 34-35 37.07340800497132 39.0 36.0 41.0 31.0 41.0 36-37 37.04651287079939 39.0 36.0 41.0 31.0 41.0 38-39 36.90105670481245 39.0 36.0 41.0 30.0 41.0 40-41 36.738275433048805 39.0 35.0 41.0 30.0 41.0 42-43 36.537766084930745 39.0 35.0 40.0 30.0 41.0 44-45 36.269807779509094 39.0 35.0 40.0 30.0 41.0 46-47 35.91819409137594 38.0 35.0 40.0 28.5 41.0 48-49 35.59422575036233 38.0 34.5 40.0 28.0 41.0 50-51 35.23624537672973 38.0 34.0 40.0 27.0 41.0 52-53 34.754063432164486 38.0 33.0 40.0 26.0 41.0 54-55 34.42337802964868 38.0 33.0 40.0 25.0 41.0 56-57 34.09130456720348 37.0 33.0 40.0 24.0 41.0 58-59 33.632568437014314 37.0 32.0 39.5 23.0 41.0 60-61 33.235018313826686 36.0 32.0 39.0 22.5 40.0 62-63 32.752300929666546 36.0 31.0 39.0 21.5 40.0 64-65 32.227337593834704 35.0 31.0 38.5 20.0 40.0 66-67 31.726656924963436 35.0 30.0 38.0 18.5 40.0 68-69 31.23656654090088 34.5 30.0 37.5 17.5 40.0 70-71 30.653246762300007 34.0 29.0 37.0 13.0 39.0 72-73 30.14756257141131 34.0 29.0 36.0 9.5 39.0 74-75 29.55192967384326 34.0 28.0 36.0 7.5 38.5 76-77 28.33474402136713 31.5 26.5 34.5 7.0 36.5 78-79 29.033697009571522 33.0 28.0 35.0 4.5 37.0 80-81 28.66145727869216 33.0 27.5 35.0 2.0 37.0 82-83 28.215731202020397 33.0 26.5 35.0 2.0 36.0 84-85 27.660551711912916 33.0 26.0 35.0 2.0 36.0 86-87 27.048110060886753 32.0 25.5 35.0 2.0 35.0 88-89 26.302736982643523 32.0 24.5 34.0 2.0 35.0 90-91 25.702276331976535 31.5 23.0 34.0 2.0 35.0 92-93 24.874398916772172 31.0 19.5 34.0 2.0 35.0 94-95 24.058780844208655 31.0 13.0 34.0 2.0 35.0 96-97 22.988618340117338 30.0 2.0 34.0 2.0 35.0 98-99 21.62828525220765 30.0 2.0 34.0 2.0 35.0 100-101 17.692718419852362 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30240369201894524 1101 2 0.3217196670109601 1101 3 0.27221208376717243 1101 4 0.23336205522747377 1101 5 0.08077573797800852 1101 6 0.1912334384394967 1101 7 0.2167447527592259 1101 8 0.14262778860913983 1101 9 0.32903543717338835 1101 10-11 0.2777088281873361 1101 12-13 0.3544332707039146 1101 14-15 0.37267411473371936 1101 16-17 0.43203802734205254 1101 18-19 0.41792536384677703 1101 20-21 0.32252365351887136 1101 22-23 0.4051241453582506 1101 24-25 0.4238782184380412 1101 26-27 0.33397608388290934 1101 28-29 0.3188378986559499 1101 30-31 0.4444798466455353 1101 32-33 0.4772404804522026 1101 34-35 0.41541667920031955 1101 36-37 0.5032693197772389 1101 38-39 0.5835932051503647 1101 40-41 0.6057804167844694 1101 42-43 0.6861432680001442 1101 44-45 0.643365144770577 1101 46-47 0.5271489697212743 1101 48-49 0.5362475862326335 1101 50-51 0.6868926984685615 1101 52-53 0.6676424833611634 1101 54-55 0.6531590844077328 1101 56-57 0.6185976460290163 1101 58-59 0.799516230904942 1101 60-61 0.7802035839727566 1101 62-63 0.7631632431265558 1101 64-65 0.5928329397671703 1101 66-67 0.4787008382664304 1101 68-69 0.4853035757475581 1101 70-71 0.5101197512778519 1101 72-73 0.5976497225363886 1101 74-75 0.6091172105927889 1101 76-77 0.5781392480920964 1101 78-79 0.6887464412485436 1101 80-81 0.6452166308842173 1101 82-83 0.7248549770090662 1101 84-85 0.8303867357758321 1101 86-87 1.0855131077075377 1101 88-89 0.9876548398733078 1101 90-91 1.0577354856962131 1101 92-93 1.2684712015193398 1101 94-95 1.1292713183240402 1101 96-97 1.2541158865599868 1101 98-99 1.4037919397431828 1101 100-101 1.710196810299081 1102 1 0.3209393477565463 1102 2 0.3209355287938962 1102 3 0.2841778018556269 1102 4 0.18799585112491002 1102 5 0.23704314066522159 1102 6 0.14861774419451024 1102 7 0.19822450395220415 1102 8 0.24015763518841737 1102 9 0.26087405802448416 1102 10-11 0.37274716286711396 1102 12-13 0.39752229319132937 1102 14-15 0.5404290615388092 1102 16-17 0.5045609345900886 1102 18-19 0.4167185911588902 1102 20-21 0.454605219300376 1102 22-23 0.4815868781054107 1102 24-25 0.48557092323156326 1102 26-27 0.4181658893157376 1102 28-29 0.3827869732833946 1102 30-31 0.38197517176034523 1102 32-33 0.49495640050812995 1102 34-35 0.565887783377768 1102 36-37 0.543888946765513 1102 38-39 0.49411931952495536 1102 40-41 0.4227772680551567 1102 42-43 0.3505953540612694 1102 44-45 0.40051296656606894 1102 46-47 0.40349934369965723 1102 48-49 0.4749043329896665 1102 50-51 0.6152281063533849 1102 52-53 0.4631605202796507 1102 54-55 0.32601037050126536 1102 56-57 0.5474513604536071 1102 58-59 0.5827296593868425 1102 60-61 0.6561229987678345 1102 62-63 0.6744311266591012 1102 64-65 0.6404246377177785 1102 66-67 0.5127728303800687 1102 68-69 0.6269678174793185 1102 70-71 0.5413738282126062 1102 72-73 0.8156315736691582 1102 74-75 0.8628543231302253 1102 76-77 0.7558335288655194 1102 78-79 0.947314682644329 1102 80-81 1.0309842426832425 1102 82-83 0.9867912479801966 1102 84-85 0.8617087771543694 1102 86-87 0.7556396657023292 1102 88-89 0.8359780191282269 1102 90-91 0.8541847721689493 1102 92-93 1.270454781707322 1102 94-95 1.2532158503919533 1102 96-97 1.081411836053089 1102 98-99 1.2397227904567387 1102 100-101 1.4111625142231077 1103 1 0.24884929943630496 1103 2 0.24918449278770538 1103 3 0.22013119175909424 1103 4 0.1632968744671217 1103 5 0.0705118612279989 1103 6 0.1636646662298702 1103 7 0.2418972458066193 1103 8 0.22531150818739576 1103 9 0.29897975458317205 1103 10-11 0.3305853709916988 1103 12-13 0.36293068784868154 1103 14-15 0.51204340018041 1103 16-17 0.4861782145048963 1103 18-19 0.45809804235791773 1103 20-21 0.42314677645102705 1103 22-23 0.30596520017375184 1103 24-25 0.3299336352968325 1103 26-27 0.4050822107459666 1103 28-29 0.4178676825565262 1103 30-31 0.33881801360735864 1103 32-33 0.39640397715191966 1103 34-35 0.3105623511489384 1103 36-37 0.43411913434559324 1103 38-39 0.2025087640470531 1103 40-41 0.4249712892553603 1103 42-43 0.5227664684784585 1103 44-45 0.4824046861517033 1103 46-47 0.4687368903999456 1103 48-49 0.5312150501841728 1103 50-51 0.7886873488205737 1103 52-53 0.650165887361112 1103 54-55 0.5813116384548138 1103 56-57 0.6410007695094322 1103 58-59 0.6459093835293714 1103 60-61 0.7415106418342958 1103 62-63 0.7286140814524416 1103 64-65 0.5015794430896818 1103 66-67 0.6881953163128394 1103 68-69 0.7595465683774663 1103 70-71 0.6160875706081335 1103 72-73 0.7201740180702494 1103 74-75 0.8918021155900959 1103 76-77 0.8260014189253049 1103 78-79 1.0059275962467744 1103 80-81 0.8190456776517188 1103 82-83 1.0522209808206817 1103 84-85 1.0553807531140649 1103 86-87 1.0914744072330471 1103 88-89 0.9572952084299722 1103 90-91 1.0487248808880025 1103 92-93 1.3419042689318985 1103 94-95 1.3351314373944483 1103 96-97 1.19727917976277 1103 98-99 1.5253435742508294 1103 100-101 1.6222817500192903 1104 1 0.21328709880929964 1104 2 0.20366908399005723 1104 3 0.19615889894122063 1104 4 0.1758535292354253 1104 5 0.15242913403961467 1104 6 0.10931951363042458 1104 7 0.1271468208459936 1104 8 0.1759338985575809 1104 9 0.23746492296149313 1104 10-11 0.1645117040071966 1104 12-13 0.23286042374052585 1104 14-15 0.2551343279634253 1104 16-17 0.350567277612889 1104 18-19 0.34665897078149044 1104 20-21 0.3036865659185608 1104 22-23 0.34394404024608605 1104 24-25 0.37119214334253314 1104 26-27 0.2917009260644008 1104 28-29 0.25555688606052485 1104 30-31 0.24464771888516879 1104 32-33 0.39938907338324725 1104 34-35 0.4099021548407151 1104 36-37 0.3734006551176634 1104 38-39 0.31570603399558195 1104 40-41 0.371162623090342 1104 42-43 0.4618171302590355 1104 44-45 0.3989978571022519 1104 46-47 0.4384692307413758 1104 48-49 0.41708858846664043 1104 50-51 0.5748373822042296 1104 52-53 0.560577895721508 1104 54-55 0.5854574123162664 1104 56-57 0.42016576547352713 1104 58-59 0.591989362521268 1104 60-61 0.645085155092822 1104 62-63 0.6527345320600091 1104 64-65 0.6380914196050966 1104 66-67 0.49275029927680336 1104 68-69 0.6535907255307194 1104 70-71 0.7101785948151047 1104 72-73 0.5825690088575435 1104 74-75 0.6715398994395159 1104 76-77 0.7451022393950844 1104 78-79 0.9749133918366688 1104 80-81 0.7415970735536312 1104 82-83 0.9714439838818585 1104 84-85 1.0143349248403304 1104 86-87 0.9367280466672057 1104 88-89 0.8582845682866527 1104 90-91 1.0633209705435824 1104 92-93 1.2650213292005148 1104 94-95 1.241349174978609 1104 96-97 1.3073126386447917 1104 98-99 1.3566492502476741 1104 100-101 1.4889705128909156 1105 1 0.16053909274680223 1105 2 0.1817467449186907 1105 3 0.18095725025807496 1105 4 0.1431468865640042 1105 5 0.05145159472245098 1105 6 0.11078995378131395 1105 7 0.14337308702965856 1105 8 0.1565457772353014 1105 9 0.26085253821482723 1105 10-11 0.2309670352456834 1105 12-13 0.2444929092362358 1105 14-15 0.3292142593170695 1105 16-17 0.20806088504969722 1105 18-19 0.2778480090525193 1105 20-21 0.20862370750685244 1105 22-23 0.2027673696806218 1105 24-25 0.23368866842177027 1105 26-27 0.13773934427186418 1105 28-29 0.2259724005166177 1105 30-31 0.35910762798210527 1105 32-33 0.37179841775714806 1105 34-35 0.32743131190024855 1105 36-37 0.4768201062394084 1105 38-39 0.41646475012962725 1105 40-41 0.44994036758921396 1105 42-43 0.4604330790049218 1105 44-45 0.4015736017680851 1105 46-47 0.3706351198003901 1105 48-49 0.43714339969345417 1105 50-51 0.46354040407278063 1105 52-53 0.4599210603910038 1105 54-55 0.44280061915818436 1105 56-57 0.4648938050501883 1105 58-59 0.34277234930925005 1105 60-61 0.34441769059294813 1105 62-63 0.418374365022828 1105 64-65 0.40823939743789595 1105 66-67 0.42123546190309114 1105 68-69 0.473161758935575 1105 70-71 0.47314381462374655 1105 72-73 0.559605914882777 1105 74-75 0.6813530541339539 1105 76-77 0.6440724148925696 1105 78-79 0.7519846382552409 1105 80-81 0.6428059371620201 1105 82-83 0.811045407243828 1105 84-85 0.9198009429124987 1105 86-87 0.924530214157091 1105 88-89 1.0325117157876775 1105 90-91 1.0876927522075341 1105 92-93 1.2235547663296238 1105 94-95 1.177721331890968 1105 96-97 1.3290314312744336 1105 98-99 1.4188451677859781 1105 100-101 1.5606438195609584 1106 1 0.007850788042741641 1106 2 0.06522021934215161 1106 3 0.04511687823567456 1106 4 0.030966632854848797 1106 5 -0.012218013958076313 1106 6 0.017703369997185803 1106 7 0.022215578567411853 1106 8 0.052921722317705644 1106 9 -0.15653381463702942 1106 10-11 0.031077756197248618 1106 12-13 0.030337077405540924 1106 14-15 0.1089425184673658 1106 16-17 0.13499992525721183 1106 18-19 0.10240813052922704 1106 20-21 0.1844201074941978 1106 22-23 0.1491439474541849 1106 24-25 0.14450594721713372 1106 26-27 0.04140420776874976 1106 28-29 0.04516872458103194 1106 30-31 0.05459037133093858 1106 32-33 -0.03256430408018218 1106 34-35 -0.04883533578567523 1106 36-37 0.10467907938166832 1106 38-39 -0.08752215316353329 1106 40-41 -0.045490178739598264 1106 42-43 0.07405697574972692 1106 44-45 0.09159511326724612 1106 46-47 -0.6396084662930619 1106 48-49 -0.3065314286890697 1106 50-51 0.14473223603000918 1106 52-53 -0.027953888202837618 1106 54-55 0.21278764898956837 1106 56-57 0.058176997609734826 1106 58-59 -0.037509830944458145 1106 60-61 0.22861701994033012 1106 62-63 -0.027696464751613803 1106 64-65 0.05978395471226605 1106 66-67 -0.07564299812664288 1106 68-69 -0.24857893259585495 1106 70-71 -0.13616022510925774 1106 72-73 0.1947715217917647 1106 74-75 0.29711987580995114 1106 76-77 0.22508535342662483 1106 78-79 0.4435068931665036 1106 80-81 0.07149958810631674 1106 82-83 0.564439004409369 1106 84-85 0.6933402172157486 1106 86-87 0.8371196608686837 1106 88-89 0.9038748488742634 1106 90-91 0.8935296444285967 1106 92-93 0.904313521193437 1106 94-95 1.0478326855336881 1106 96-97 1.3101155475630684 1106 98-99 1.1134438834229385 1106 100-101 1.1129401720964687 1107 1 0.09125502177306899 1107 2 0.1881120423321434 1107 3 0.14326636519076175 1107 4 0.07538706333060219 1107 5 0.08497108336191417 1107 6 0.15856914121339827 1107 7 0.15379707597364956 1107 8 0.13487547851673298 1107 9 0.15521992380519123 1107 10-11 0.14767022477403913 1107 12-13 0.2469034181079408 1107 14-15 0.30196891269386583 1107 16-17 0.22654438703568758 1107 18-19 0.24934368600022339 1107 20-21 0.29798250364857637 1107 22-23 0.2919660115562621 1107 24-25 0.4139507933036555 1107 26-27 0.2885244340412143 1107 28-29 0.26637897786920917 1107 30-31 0.2186589633429108 1107 32-33 0.2279176664181506 1107 34-35 0.2710233707577032 1107 36-37 0.3584936222072841 1107 38-39 0.2718964382142701 1107 40-41 0.3150407498146137 1107 42-43 0.2934852065374969 1107 44-45 0.26063916283410293 1107 46-47 0.2769926270189629 1107 48-49 0.451749458643981 1107 50-51 0.423795183913775 1107 52-53 0.4396427278569206 1107 54-55 0.3004735564258638 1107 56-57 0.4002606321746356 1107 58-59 0.33433040851878104 1107 60-61 0.42911294221624274 1107 62-63 0.706077744753351 1107 64-65 0.5166831087272001 1107 66-67 0.44969977168170416 1107 68-69 0.6380141701526441 1107 70-71 0.8965551713114763 1107 72-73 0.9298176260749607 1107 74-75 0.8204606923694442 1107 76-77 0.9449082926856569 1107 78-79 1.1901492076395712 1107 80-81 1.1105927225633998 1107 82-83 1.1542618168340617 1107 84-85 1.2800150012802831 1107 86-87 1.6411806428201565 1107 88-89 1.5200160408634034 1107 90-91 1.6040990037883347 1107 92-93 1.8141315139131464 1107 94-95 1.8385227911866941 1107 96-97 1.8472539111463924 1107 98-99 1.9371735740739773 1107 100-101 1.916782998980274 1108 1 0.09165003757370016 1108 2 0.11132247074927903 1108 3 0.12705871688483228 1108 4 0.14949852778918427 1108 5 0.05630293663604391 1108 6 0.08866334498155481 1108 7 0.009154290262223697 1108 8 0.07756368604503905 1108 9 0.1005487369577196 1108 10-11 0.1371010520071252 1108 12-13 0.18475468215850555 1108 14-15 0.3115355453591704 1108 16-17 0.22221146371875733 1108 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-0.8354378621568088 2316 76-77 -0.953040584683535 2316 78-79 -0.9558670632828559 2316 80-81 -1.1250021696485213 2316 82-83 -1.1492761971308063 2316 84-85 -1.2071688742346396 2316 86-87 -1.1619317221382808 2316 88-89 -1.252377424298455 2316 90-91 -1.3344938551533616 2316 92-93 -1.4617875875442294 2316 94-95 -1.3474146530245328 2316 96-97 -1.2870095246946747 2316 98-99 -1.4169454524508929 2316 100-101 -1.8177706456006604 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 8.0 3 19.0 4 36.0 5 84.0 6 195.0 7 569.0 8 1110.0 9 2053.0 10 3400.0 11 5342.0 12 7498.0 13 10866.0 14 14297.0 15 18577.0 16 23105.0 17 28641.0 18 28296.0 19 25428.0 20 25876.0 21 28049.0 22 31453.0 23 35065.0 24 40862.0 25 47606.0 26 57151.0 27 68941.0 28 82009.0 29 97628.0 30 115301.0 31 136580.0 32 164641.0 33 201059.0 34 252845.0 35 325724.0 36 425712.0 37 471163.0 38 225966.0 39 9322.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.46424189633469 19.17191375564986 14.827330716649229 23.53651363136622 2 16.056174774822647 20.39057310625183 39.21350665568457 24.339745463240963 3 19.62497319482499 23.81066351355927 29.519418897001078 27.044944394614667 4 13.51797423914797 16.672852050703774 35.984959890163516 33.82421381998474 5 14.059443622676199 37.279625335672215 33.92728690501414 14.733644136637439 6 33.234909345365956 35.73866290099476 16.77941441544616 14.24701333819312 7 29.481974640146753 32.03405966782164 19.98305049862605 18.50091519340556 8 26.723016732404353 35.40400958944762 19.110844958867748 18.762128719280273 9 26.38024244492408 16.739806896515095 19.534881250585066 37.345069407975764 10-11 25.37261682906896 26.440325871259475 26.974620229591718 21.212437070079847 12-13 26.132638976948545 23.434030057646893 27.46300553896161 22.970325426442948 14-15 22.726622401889742 25.195453709537464 25.506493325428437 26.57143056314436 16-17 22.666953916343953 28.350099154251083 26.310150646743313 22.67279628266165 18-19 22.653393651339528 26.943981001686986 28.380954151366417 22.02167119560707 20-21 23.246045282322395 26.131277971158628 28.47277224929112 22.149904497227862 22-23 22.966922911968155 26.066348036400598 28.336074155562297 22.63065489606895 24-25 22.80614218110172 26.303703002869916 28.408683109333943 22.48147170669442 26-27 22.38184068557357 26.65050914935186 28.284258553694176 22.683391611380394 28-29 22.481934713847558 26.82978605808211 28.253730775991116 22.434548452079213 30-31 22.668377356486292 26.438838412842784 28.149566960892038 22.74321726977888 32-33 22.40583880254954 26.480074304548403 28.095693662370408 23.018393230531654 34-35 22.94799665418765 26.570275089982196 27.99837058473864 22.483357671091508 36-37 23.057425421724272 26.182232430949977 28.0221442179798 22.73819792934595 38-39 22.58125340992026 26.51522457297051 27.989637991993295 22.913884025115934 40-41 23.043191263455203 26.30933730175394 28.073433264192477 22.574038170598378 42-43 22.75358102033083 26.343323503553346 27.882090441832208 23.021005034283622 44-45 22.714505793981548 26.58147038040542 28.00535352574621 22.698670299866826 46-47 22.984184448815892 26.81544894269767 27.441860156323134 22.758506452163303 48-49 22.795983904280796 26.758369687679046 27.49049121686532 22.95515519117484 50-51 22.99238198112309 26.876280306181354 27.399324074685733 22.73201363800983 52-53 23.1961528017798 26.67325703291443 27.305676589173434 22.824913576132342 54-55 22.834980894180884 26.74562388607481 27.472976162457392 22.946419057286917 56-57 22.801627099019477 26.95710972827021 27.272265556794107 22.968997615916198 58-59 22.823856789273307 26.682329433744815 27.300777421975162 23.19303635500671 60-61 22.639916374493023 26.81946556954109 27.545379584514805 22.995238471451078 62-63 22.635512095040646 26.80902733397849 27.604778590956396 22.950681980024466 64-65 22.425969230503632 26.747025231890063 27.496765897813823 23.33023963979248 66-67 22.42666141261507 26.873710382356748 27.329013568964488 23.370614636063696 68-69 22.789179788930454 26.527833896028312 27.49652804637992 23.186458268661315 70-71 22.656897956514502 26.535452321520953 27.26705993127579 23.540589790688752 72-73 22.671902227918533 26.609245452784595 27.218910162090943 23.499942157205925 74-75 22.54319695213734 26.95478856523582 27.00231803707764 23.499696445549205 76-77 22.564367150522163 26.53190140048692 27.290954449012343 23.61277699997858 78-79 22.647535165961756 26.71379535049745 27.22578020910725 23.412889274433546 80-81 22.618222266494005 26.789836305076868 27.078095427831457 23.513846000597667 82-83 22.655195034516012 26.762448452619747 27.105109751069968 23.47724676179428 84-85 22.53788391258625 26.74215367044627 27.053691113621444 23.666271303346033 86-87 22.55666755141632 26.71019369377669 26.96150907416178 23.771629680645212 88-89 22.48947417206178 26.73762955663781 26.837530652227574 23.935365619072837 90-91 22.416789146044394 26.70910404861788 26.979480076494198 23.894626728843523 92-93 22.4034017126766 26.482305960899495 27.034637690639112 24.079654635784784 94-95 22.265658203706064 26.305519916911045 27.153824801309025 24.274997078073866 96-97 22.249262135251737 26.161655473614857 27.207849393237417 24.381232997895992 98-99 22.086410166652165 26.25701524639133 26.93643716816863 24.720137418787875 100-101 22.245436478557043 26.033934498834345 27.185430595497134 24.535198427111478 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 320.0 1 239.5 2 201.0 3 251.0 4 225.5 5 245.0 6 443.5 7 975.0 8 1553.5 9 1791.0 10 1621.0 11 1402.0 12 1481.0 13 1700.0 14 1932.0 15 2068.5 16 2206.0 17 2441.5 18 2692.5 19 2966.0 20 3404.5 21 3953.5 22 4683.5 23 5769.5 24 7233.5 25 9157.0 26 12100.0 27 16114.0 28 19937.5 29 24074.5 30 28738.5 31 34980.5 32 43967.5 33 49342.0 34 52376.0 35 57419.0 36 63516.0 37 69896.5 38 75014.5 39 79332.0 40 84258.0 41 91163.5 42 96171.5 43 100728.5 44 106332.5 45 111910.0 46 115926.0 47 120179.0 48 123819.5 49 123136.5 50 120899.0 51 116900.5 52 112706.5 53 112020.5 54 112137.0 55 105672.0 56 95721.5 57 86603.0 58 78168.0 59 67782.5 60 55080.5 61 45476.5 62 37719.5 63 28129.5 64 19764.5 65 14581.0 66 10755.0 67 8137.0 68 6132.5 69 4476.5 70 3312.5 71 2383.0 72 1822.5 73 1436.5 74 1096.5 75 852.0 76 603.5 77 357.0 78 215.0 79 129.0 80 70.0 81 48.5 82 32.5 83 13.5 84 4.0 85 3.0 86 2.0 87 1.5 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 4.6473368436217625E-4 2 0.0503904094901274 3 0.0 4 0.0 5 0.04501277685679364 6 3.319526316872687E-5 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.006406685791564287 26-27 0.0017095560531894339 28-29 4.979289475309031E-5 30-31 0.00159337263209889 32-33 3.651478948559956E-4 34-35 0.011767720793313678 36-37 0.022439997902059368 38-39 0.0042323960540126765 40-41 9.46065000308716E-4 42-43 0.0011120413161523502 44-45 0.00854778026594717 46-47 0.0 48-49 0.0 50-51 4.979289475309032E-4 52-53 0.0 54-55 0.0014937868425927093 56-57 0.0 58-59 0.02205825237561901 60-61 0.0 62-63 0.009726212108436974 64-65 0.014489732373149282 66-67 0.006622455002161012 68-69 0.016099702636832535 70-71 8.298815792181718E-5 72-73 8.298815792181718E-5 74-75 0.021991861849281556 76-77 0.04944434448981868 78-79 0.02873050027253311 80-81 0.025825914745269508 82-83 0.034838428695578856 84-85 0.015950323952573262 86-87 0.010838253424589325 88-89 0.08302135318498592 90-91 0.024780263955454615 92-93 0.02326987948127754 94-95 0.025427571587244784 96-97 0.036050055801237386 98-99 0.05098792422716448 100-101 0.014605915794239826 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3012478.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.56147485470303 #Duplication Level Percentage of deduplicated Percentage of total 1 73.21235285235919 37.74936890650563 2 14.446962600398946 14.898133976946104 3 5.126151911331952 7.92935858632591 4 2.4478353146100735 5.048559960908854 5 1.2693385238502166 3.27244831898044 6 0.7782136218934061 2.4075505258106538 7 0.49549673134576916 1.7883979577710694 8 0.3569248574964122 1.4722857651855772 9 0.28012869458421064 1.2999463777696105 >10 1.4398913007811847 13.36250731299662 >50 0.09370203693417391 3.3201532665500126 >100 0.047847562664184655 4.381474549727464 >500 0.0032856697409981285 1.150520219310727 >1k 0.001739472215505678 1.4993469210761887 >5k 1.288497937411613E-4 0.4199473541351732 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6933 0.23014275954878344 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5709 0.18951175743026175 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3963 0.1315528279376646 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.319526316872688E-5 2 0.0 0.0 0.0 0.0 3.319526316872688E-5 3 0.0 0.0 0.0 0.0 3.319526316872688E-5 4 0.0 0.0 0.0 0.0 3.319526316872688E-5 5 0.0 0.0 0.0 0.0 3.319526316872688E-5 6 0.0 0.0 0.0 0.0 3.319526316872688E-5 7 0.0 0.0 0.0 0.0 3.319526316872688E-5 8 0.0 0.0 0.0 0.0 3.319526316872688E-5 9 0.0 0.0 0.0 0.0 3.319526316872688E-5 10-11 0.0 0.0 0.0 0.0 3.319526316872688E-5 12-13 0.0 0.0 0.0 0.0 1.6597631584363437E-4 14-15 0.0 0.0 0.0 1.659763158436344E-5 1.6597631584363437E-4 16-17 0.0 0.0 0.0 3.319526316872688E-5 1.8257394742799782E-4 18-19 0.0 0.0 0.0 3.319526316872688E-5 1.9917157901236124E-4 20-21 0.0 0.0 0.0 9.958578950618062E-5 2.3236684218108812E-4 22-23 0.0 0.0 0.0 2.489644737654516E-4 2.3236684218108812E-4 24-25 0.0 0.0 0.0 5.311242106996301E-4 2.3236684218108812E-4 26-27 0.0 0.0 0.0 9.792602634774428E-4 2.6556210534981503E-4 28-29 0.0 0.0 0.0 0.003568490790638139 2.6556210534981503E-4 30-31 0.0 0.0 0.0 0.012365235530350762 2.6556210534981503E-4 32-33 0.0 0.0 0.0 0.03219940527366507 2.6556210534981503E-4 34-35 0.0 0.0 0.0 0.06151082265165089 2.6556210534981503E-4 36-37 0.0 0.0 0.0 0.11138670556266303 2.6556210534981503E-4 38-39 0.0 0.0 0.0 0.18853249716678427 2.6556210534981503E-4 40-41 0.0 0.0 0.0 0.2731306253522847 2.6556210534981503E-4 42-43 0.0 0.0 0.0 0.35824328011690043 2.6556210534981503E-4 44-45 0.0 0.0 0.0 0.46328969041433665 2.6556210534981503E-4 46-47 4.979289475309032E-5 0.0 0.0 0.5915063944035441 2.6556210534981503E-4 48-49 6.639052633745376E-5 0.0 0.0 0.7276733639216618 2.6556210534981503E-4 50-51 6.639052633745376E-5 0.0 0.0 0.8766869002860769 2.6556210534981503E-4 52-53 6.639052633745376E-5 0.0 0.0 1.0204887803330016 3.3195263168726873E-4 54-55 1.3278105267490751E-4 0.0 0.0 1.1821165167015328 3.3195263168726873E-4 56-57 1.3278105267490751E-4 0.0 0.0 1.368773481499284 3.3195263168726873E-4 58-59 1.3278105267490751E-4 0.0 0.0 1.5747998823559872 3.3195263168726873E-4 60-61 1.3278105267490751E-4 0.0 0.0 1.7964778497967453 3.3195263168726873E-4 62-63 1.3278105267490751E-4 0.0 0.0 2.016728420921248 4.315384211934494E-4 64-65 1.3278105267490751E-4 0.0 0.0 2.247717659680834 4.315384211934494E-4 66-67 1.3278105267490751E-4 0.0 0.0 2.512732043188365 4.315384211934494E-4 68-69 1.3278105267490751E-4 0.0 0.0 2.8085018380217215 4.315384211934494E-4 70-71 1.3278105267490751E-4 0.0 0.0 3.119309087070511 4.6473368436217625E-4 72-73 1.3278105267490751E-4 0.0 0.0 3.442265802439055 4.813313159465397E-4 74-75 1.3278105267490751E-4 0.0 0.0 3.7715130201780727 5.145265791152666E-4 76-77 1.3278105267490751E-4 0.0 0.0 4.129075797400015 5.311242106996301E-4 78-79 1.3278105267490751E-4 0.0 0.0 4.529510257004366 5.311242106996301E-4 80-81 1.9917157901236124E-4 0.0 0.0 4.952484300300284 5.311242106996301E-4 82-83 1.9917157901236124E-4 0.0 0.0 5.394678400970895 5.975147370370838E-4 84-85 1.9917157901236124E-4 0.0 0.0 5.845387086644284 6.639052633745375E-4 86-87 1.9917157901236124E-4 0.0 0.0 6.308261836269011 6.80502894958901E-4 88-89 1.9917157901236124E-4 0.0 0.0 6.80552687853654 6.971005265432644E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5445 0.0 46.867764 1 GTATCAA 8890 0.0 37.63299 1 ATCAACG 11375 0.0 28.938133 3 TATCAAC 11500 0.0 28.885319 2 TCAACGC 11525 0.0 28.561499 4 CAACGCA 11780 0.0 27.902908 5 AACGCAG 12275 0.0 26.92265 6 TACATGG 11275 0.0 24.99401 2 GTACATG 11835 0.0 24.092442 1 ACGCAGA 14160 0.0 23.238071 7 ACATGGG 11620 0.0 23.054815 3 CGCAGAG 14305 0.0 22.969328 8 TAAGGTG 1500 0.0 21.216425 5 GTAAGGT 1470 0.0 20.35691 4 GAGTACT 8155 0.0 20.116581 12-13 GCAGAGT 15935 0.0 20.023834 9 CATGGGG 6300 0.0 19.301369 4 GTACTTT 8450 0.0 19.189518 14-15 AGAGTAC 13940 0.0 18.98944 10-11 TGGTATC 2715 0.0 18.903915 2 >>END_MODULE