FastQCFastQC Report
Thu 26 May 2016
SRR938454_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938454_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030413
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70840.23376351672197818No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54090.1784905225789356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39070.12892632126380135No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53350.045.7791441
GTATCAA91800.039.5181921
ATCAACG114850.031.1996543
TATCAAC115950.031.0266252
TCAACGC116750.030.5290854
CAACGCA118700.029.9855355
AACGCAG123850.028.8111176
ACGCAGA143300.024.565397
CGCAGAG144950.024.2861568
GTACATG119450.023.5153031
TACATGG116750.023.16142
ACATGGG119750.022.065243
GCAGAGT158000.021.136629
TGGTATC28600.020.770812
GAGTACT77500.020.46981612-13
GGACCGA9700.020.0829856
ACCGAGT10450.020.0030028
AGAGTAC139300.019.62501510-11
GTGGTAT31800.019.3128931
AGTACTT84450.018.9539412-13
Produced by FastQC (version 0.11.5)