##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938454_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3030413 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.914315639485444 31.0 31.0 34.0 27.0 34.0 2 31.016245970433733 31.0 31.0 34.0 27.0 34.0 3 31.08327247804177 31.0 31.0 34.0 27.0 34.0 4 34.701792461951555 37.0 35.0 37.0 32.0 37.0 5 34.71999064153962 37.0 35.0 37.0 32.0 37.0 6 34.742040771340406 37.0 35.0 37.0 31.0 37.0 7 34.65842114589662 37.0 35.0 37.0 30.0 37.0 8 34.613448067969614 37.0 35.0 37.0 30.0 37.0 9 36.093438089131745 39.0 35.0 39.0 30.0 39.0 10-11 35.94136657280707 38.0 35.0 39.0 30.0 39.0 12-13 35.78920810463788 38.0 35.0 39.0 30.0 39.0 14-15 36.752426814430905 39.0 36.0 41.0 30.0 41.0 16-17 36.60898992975545 39.0 36.0 41.0 30.0 41.0 18-19 36.585167434273814 39.0 36.0 40.5 30.0 41.0 20-21 36.67049705766178 39.0 36.0 41.0 30.0 41.0 22-23 36.59110622875496 39.0 36.0 41.0 30.0 41.0 24-25 36.469891727629204 39.0 36.0 41.0 29.5 41.0 26-27 36.28324571601297 39.0 35.0 41.0 29.0 41.0 28-29 36.098586562293654 39.0 35.0 40.0 27.5 41.0 30-31 35.885302267380716 38.5 35.0 40.0 27.0 41.0 32-33 35.61968187174487 38.0 34.5 40.0 27.0 41.0 34-35 35.37141653629389 38.0 34.0 40.0 26.0 41.0 36-37 35.0912294792822 38.0 34.0 40.0 25.0 41.0 38-39 34.83642757604326 38.0 33.5 40.0 25.0 41.0 40-41 34.79458855938118 38.0 33.5 40.0 24.5 41.0 42-43 34.62143592309035 38.0 33.0 40.0 24.0 41.0 44-45 34.349378946038044 38.0 33.0 40.0 23.5 41.0 46-47 34.00627125741607 38.0 33.0 40.0 22.5 41.0 48-49 33.65796939229075 38.0 32.5 40.0 20.5 41.0 50-51 32.928516674129895 37.0 31.5 39.5 19.0 40.5 52-53 33.00690631936967 37.0 31.5 39.5 19.0 40.0 54-55 33.14272196561987 37.0 32.0 40.0 18.5 41.0 56-57 32.847264712763575 37.0 31.5 40.0 16.0 41.0 58-59 32.52138338899681 36.5 31.0 40.0 13.5 41.0 60-61 32.075744461233505 36.0 31.0 39.0 10.0 41.0 62-63 31.552911104856005 35.5 30.0 39.0 8.0 40.0 64-65 31.065294400466207 35.0 29.5 39.0 6.5 40.0 66-67 30.525705572144787 35.0 29.0 38.0 2.0 40.0 68-69 29.968189154415583 34.0 28.0 37.5 2.0 40.0 70-71 29.343061490298517 34.0 27.5 37.0 2.0 39.0 72-73 28.792271548465507 34.0 26.5 36.0 2.0 39.0 74-75 28.196496649136602 33.5 26.0 36.0 2.0 39.0 76-77 27.478727321985485 33.0 25.5 35.5 2.0 38.0 78-79 26.710668809828892 32.0 23.5 35.0 2.0 37.0 80-81 26.058602738306625 32.0 21.5 35.0 2.0 36.5 82-83 25.41451973707874 31.0 20.0 35.0 2.0 36.0 84-85 24.776402094367995 31.0 18.0 34.0 2.0 35.5 86-87 24.126850201606185 31.0 11.5 34.0 2.0 35.0 88-89 23.34729622661994 30.0 4.5 34.0 2.0 35.0 90-91 22.53865826209167 29.5 2.0 34.0 2.0 35.0 92-93 21.709986559587755 29.0 2.0 34.0 2.0 35.0 94-95 20.76860546730759 29.0 2.0 34.0 2.0 35.0 96-97 19.55446864833275 26.5 2.0 33.5 2.0 35.0 98-99 18.11423987423496 24.5 2.0 33.0 2.0 34.5 100-101 14.7841960815242 2.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.7185143096407174 1101 2 0.7041025480282812 1101 3 0.8136305716550787 1101 4 0.7154649010346574 1101 5 0.6826031637715388 1101 6 0.6005060506113367 1101 7 0.517332252085076 1101 8 0.605140009520774 1101 9 0.7692289146497728 1101 10-11 0.7131933765845986 1101 12-13 0.806290386234366 1101 14-15 1.0118162798034547 1101 16-17 0.962618343107593 1101 18-19 0.8574151268846109 1101 20-21 0.9836233129661736 1101 22-23 1.0806799949954708 1101 24-25 1.1281922215098845 1101 26-27 1.1295915157395129 1101 28-29 1.1243818744593241 1101 30-31 1.027444762922336 1101 32-33 1.1013230745458884 1101 34-35 1.1034851615318146 1101 36-37 1.103951720358289 1101 38-39 1.1500679178757878 1101 40-41 1.2367638434101735 1101 42-43 1.1862775300280575 1101 44-45 1.3245472352987449 1101 46-47 1.3349153531770597 1101 48-49 1.4773116198084821 1101 50-51 1.280358004781462 1101 52-53 1.3789026947977803 1101 54-55 1.466638337219834 1101 56-57 1.3977382406473993 1101 58-59 1.5599044246693126 1101 60-61 1.5127917704299776 1101 62-63 1.4079961162848242 1101 64-65 1.3334167116731912 1101 66-67 1.3244635408128858 1101 68-69 1.4703164594178517 1101 70-71 1.6018188392614334 1101 72-73 1.4526222545505263 1101 74-75 1.4731376206574183 1101 76-77 1.4771336213082442 1101 78-79 1.5323224143181733 1101 80-81 1.5148175657670286 1101 82-83 1.4711124184184037 1101 84-85 1.4894186742936846 1101 86-87 1.7007438186681405 1101 88-89 1.7016274584651647 1101 90-91 1.639544612906402 1101 92-93 1.6408961561340796 1101 94-95 1.5717189445142843 1101 96-97 1.4849195174205043 1101 98-99 1.3793027205263861 1101 100-101 1.1671459898194971 1102 1 0.5733417785558252 1102 2 0.5822546107542159 1102 3 0.5566975481775458 1102 4 0.5229230407081147 1102 5 0.5044241624606087 1102 6 0.4862901744742061 1102 7 0.47930452892931186 1102 8 0.5573292350799051 1102 9 0.7392886016159679 1102 10-11 0.7639680160792963 1102 12-13 0.7343153286329667 1102 14-15 0.7241727728714267 1102 16-17 0.7070894778551349 1102 18-19 0.8327938188363078 1102 20-21 0.6664835451593945 1102 22-23 0.7228296940778165 1102 24-25 0.5352050676148465 1102 26-27 0.6615561330874655 1102 28-29 0.5074120556057906 1102 30-31 0.5787456358814609 1102 32-33 0.6540521890733544 1102 34-35 0.6129266390076893 1102 36-37 0.6171655307901034 1102 38-39 0.7071633177089467 1102 40-41 0.7543716846488024 1102 42-43 0.8457815124695642 1102 44-45 0.9829767157937184 1102 46-47 0.8635721729839929 1102 48-49 0.8356762366598716 1102 50-51 0.8559291151973767 1102 52-53 0.8185036561452463 1102 54-55 0.7657304944915211 1102 56-57 0.8840438185483279 1102 58-59 0.9613751224407352 1102 60-61 0.8625077980190596 1102 62-63 0.7912583789273349 1102 64-65 0.9981263584177178 1102 66-67 0.9778375054774031 1102 68-69 0.9302628181156614 1102 70-71 0.9344690870467574 1102 72-73 1.0508060104596346 1102 74-75 0.8141977346684612 1102 76-77 0.8991832876173866 1102 78-79 0.8567832701955034 1102 80-81 1.0448259080385078 1102 82-83 1.0718852578694005 1102 84-85 0.77358008979839 1102 86-87 0.5094858239913016 1102 88-89 0.9094424412840567 1102 90-91 1.0908776383701948 1102 92-93 0.8951978881485374 1102 94-95 0.8869690388613982 1102 96-97 1.0812550629454734 1102 98-99 1.145630358273177 1102 100-101 0.8910223901293559 1103 1 0.7217546437579472 1103 2 0.6886626937699845 1103 3 0.7258692585563828 1103 4 0.774056474915696 1103 5 0.7330948640970121 1103 6 0.666460446461052 1103 7 0.5448720866517931 1103 8 0.5742497218915332 1103 9 0.716092195727903 1103 10-11 0.6908790225800772 1103 12-13 0.7305313769778863 1103 14-15 1.0444559679696823 1103 16-17 1.028725835547732 1103 18-19 0.9391760995282326 1103 20-21 0.7208026907171998 1103 22-23 0.9101062296079689 1103 24-25 0.9725637969729846 1103 26-27 0.9133794583882135 1103 28-29 0.8450451535658843 1103 30-31 0.9461807681388024 1103 32-33 1.1114720500910664 1103 34-35 1.1270584900500538 1103 36-37 1.1600599677623862 1103 38-39 1.0908861377044587 1103 40-41 1.11774954742247 1103 42-43 1.2729554458178356 1103 44-45 1.2211633378141897 1103 46-47 1.1991912886531182 1103 48-49 1.3869175502666806 1103 50-51 1.4098320377483802 1103 52-53 1.426078564611224 1103 54-55 1.4140144648270194 1103 56-57 1.4353235437594165 1103 58-59 1.4161169123981452 1103 60-61 1.6431311868332301 1103 62-63 1.696438808390095 1103 64-65 1.5228147457374526 1103 66-67 1.5512357766049547 1103 68-69 1.6844078855519662 1103 70-71 1.7049912747102098 1103 72-73 1.5634245143451615 1103 74-75 1.5776611770954219 1103 76-77 1.6355974884692124 1103 78-79 1.390226117464426 1103 80-81 1.6224878672404834 1103 82-83 1.7597503432389026 1103 84-85 1.7464823958956792 1103 86-87 1.7350079416031967 1103 88-89 1.556207577090678 1103 90-91 1.7931328315256891 1103 92-93 1.8013726994763424 1103 94-95 1.6367114826333484 1103 96-97 1.5922128778972748 1103 98-99 1.8083612949092114 1103 100-101 1.5759550005306107 1104 1 0.5936333719060229 1104 2 0.7140807178329212 1104 3 0.6420131755832408 1104 4 0.6322770767798218 1104 5 0.6528130330203794 1104 6 0.5947607470758385 1104 7 0.5573339884036344 1104 8 0.4580906953607098 1104 9 0.5647976928583134 1104 10-11 0.6454537743548912 1104 12-13 0.7524514177491355 1104 14-15 0.8818233465728085 1104 16-17 0.7863275135236023 1104 18-19 0.8376602568472364 1104 20-21 0.8259222388431908 1104 22-23 0.9016942739744422 1104 24-25 1.0321091650890892 1104 26-27 0.9908548266796018 1104 28-29 1.0053754615224264 1104 30-31 0.9319682384328729 1104 32-33 0.8225499345918905 1104 34-35 0.8379848912346759 1104 36-37 0.9108568776136678 1104 38-39 1.0696110797236855 1104 40-41 1.0375344999096257 1104 42-43 0.9633598236246641 1104 44-45 1.1002258474517745 1104 46-47 1.1749514619975159 1104 48-49 1.0806875090672108 1104 50-51 0.9111664887264723 1104 52-53 0.8947445374092311 1104 54-55 1.1229873358346794 1104 56-57 1.1811611638505752 1104 58-59 1.0668989457113796 1104 60-61 1.1860779550079528 1104 62-63 1.2607052863469654 1104 64-65 1.1384877055162228 1104 66-67 1.260499133927862 1104 68-69 1.222664883348994 1104 70-71 1.3798560490871559 1104 72-73 1.3542770298335114 1104 74-75 1.2044789491557992 1104 76-77 1.5048957862427024 1104 78-79 1.2538392702321595 1104 80-81 1.199681291300358 1104 82-83 1.3470185449217134 1104 84-85 1.3126133777671605 1104 86-87 1.2434428055522098 1104 88-89 1.238867834901857 1104 90-91 1.3120287527805452 1104 92-93 1.4458554452437404 1104 94-95 1.2420545671065675 1104 96-97 1.1173619927266216 1104 98-99 1.121892432579422 1104 100-101 1.1899456690252084 1105 1 0.5504068015616852 1105 2 0.6255531432320218 1105 3 0.5246987344171643 1105 4 0.6068205118609242 1105 5 0.5156718708613397 1105 6 0.5089309505624868 1105 7 0.44584279863706655 1105 8 0.4436320991296583 1105 9 0.5929610017741354 1105 10-11 0.6670037111078742 1105 12-13 0.7291631619483283 1105 14-15 0.8327694154538321 1105 16-17 0.7503682396725964 1105 18-19 0.7673855659336155 1105 20-21 0.7094807997336119 1105 22-23 0.6677750326953102 1105 24-25 0.7923938369810983 1105 26-27 0.875864530675436 1105 28-29 0.8921425904920213 1105 30-31 0.7612287184868762 1105 32-33 0.918761560366363 1105 34-35 1.0018971380371298 1105 36-37 0.9926546299669283 1105 38-39 0.8916761506648356 1105 40-41 1.0096915087797882 1105 42-43 1.0455296612168183 1105 44-45 0.9788912099344103 1105 46-47 0.9549190267529966 1105 48-49 0.86637153483737 1105 50-51 0.7738216189717804 1105 52-53 0.7406171397356047 1105 54-55 1.0890723426887945 1105 56-57 1.0318553974152849 1105 58-59 0.9714948922024078 1105 60-61 1.110493044163782 1105 62-63 1.115488615006992 1105 64-65 1.1568896474467998 1105 66-67 1.2713197590630827 1105 68-69 1.1814591115661806 1105 70-71 1.1856752658274488 1105 72-73 1.2463730577115122 1105 74-75 1.170468853328373 1105 76-77 1.189878720594848 1105 78-79 1.2499265580563197 1105 80-81 1.3378917982574237 1105 82-83 1.4271444220198006 1105 84-85 1.419638370559742 1105 86-87 1.5549534001083423 1105 88-89 1.4355190997728577 1105 90-91 1.4124889921212294 1105 92-93 1.459619236867038 1105 94-95 1.5962033975730705 1105 96-97 1.6921524599583009 1105 98-99 1.6290325519178097 1105 100-101 1.341960260829234 1106 1 0.28301374940776114 1106 2 0.24027497900714678 1106 3 0.17507117335450317 1106 4 0.24013623356447766 1106 5 0.3367556445470754 1106 6 -0.08823498977857724 1106 7 0.1614471429987887 1106 8 0.2998594766494733 1106 9 0.29920683821257654 1106 10-11 0.048771457220532 1106 12-13 0.1203893467461441 1106 14-15 0.5440047086948638 1106 16-17 0.33187633490983615 1106 18-19 -0.2210164941514634 1106 20-21 0.12322369452961368 1106 22-23 0.25443122680133 1106 24-25 0.273540057088141 1106 26-27 -0.04278369095333545 1106 28-29 0.07357167119982222 1106 30-31 0.13814990191652754 1106 32-33 0.20557674531859504 1106 34-35 0.2200745277695333 1106 36-37 0.08356789566723677 1106 38-39 0.12147372825016589 1106 40-41 0.2858091347377396 1106 42-43 -0.06465602609808485 1106 44-45 0.12937557555269308 1106 46-47 -0.1836928139483831 1106 48-49 0.14595201799494362 1106 50-51 -0.12363145188309232 1106 52-53 0.1898429062087743 1106 54-55 -0.02489427863999083 1106 56-57 -0.3881795947334581 1106 58-59 -0.8054293668329215 1106 60-61 -0.1385108982131058 1106 62-63 0.016689485773323298 1106 64-65 0.1837964939035821 1106 66-67 -0.5516624877165412 1106 68-69 -0.545689745952 1106 70-71 -0.5783623110983669 1106 72-73 -0.10350329976941097 1106 74-75 -0.25511353797300984 1106 76-77 -0.14084380048018375 1106 78-79 0.1286197025523954 1106 80-81 -9.543380684284841E-4 1106 82-83 0.13657572984660504 1106 84-85 0.22568333084162262 1106 86-87 0.17596146739033713 1106 88-89 0.0030542216572122527 1106 90-91 0.04259435222550678 1106 92-93 0.11900864352985607 1106 94-95 -0.20360468886011418 1106 96-97 -0.33924086022778255 1106 98-99 -0.49807324712084977 1106 100-101 -0.8299208609868369 1107 1 0.4967068678913904 1107 2 0.4478028380015644 1107 3 0.43668079775878255 1107 4 0.5610616504233619 1107 5 0.4786913316398369 1107 6 0.5720488288572341 1107 7 0.48455473830954787 1107 8 0.5008157649514899 1107 9 0.4778424821296454 1107 10-11 0.6154546157442908 1107 12-13 0.5376636675497721 1107 14-15 0.5470964188792422 1107 16-17 0.6609717363699303 1107 18-19 0.6437662155420583 1107 20-21 0.6417679533112732 1107 22-23 0.7424957326037855 1107 24-25 0.7191543278046808 1107 26-27 0.6622793673141487 1107 28-29 0.683584584919025 1107 30-31 0.7770130144409251 1107 32-33 0.779638453729973 1107 34-35 0.8404156465453738 1107 36-37 0.8775054247041183 1107 38-39 1.0925485773404375 1107 40-41 1.0304177134036863 1107 42-43 1.0782912835471663 1107 44-45 1.1783537746993602 1107 46-47 1.0899697860493163 1107 48-49 1.191444662120503 1107 50-51 1.0372393120147976 1107 52-53 1.0160573970721103 1107 54-55 1.0756743107952644 1107 56-57 1.2438541549525723 1107 58-59 1.0820357170806005 1107 60-61 1.024272349945356 1107 62-63 1.0549405048346578 1107 64-65 0.9903470102881862 1107 66-67 1.0582264292630015 1107 68-69 1.0064868200698776 1107 70-71 1.0270631336687543 1107 72-73 0.8882423239716388 1107 74-75 1.0997853951933116 1107 76-77 1.0746020480863976 1107 78-79 0.9630210563484916 1107 80-81 0.8673656902830622 1107 82-83 0.9846775177800673 1107 84-85 0.9781838377045879 1107 86-87 1.1109026302447909 1107 88-89 0.9850958820992446 1107 90-91 1.0559460359134647 1107 92-93 1.0617354370039251 1107 94-95 1.0768751667229317 1107 96-97 1.119898802476456 1107 98-99 1.0873615838454853 1107 100-101 0.6617772058747473 1108 1 0.40681922963789674 1108 2 0.39698422252217824 1108 3 0.4431681974204409 1108 4 0.3870707797664039 1108 5 0.4014579728733878 1108 6 0.408088470125179 1108 7 0.3869734513520484 1108 8 0.3108258730526643 1108 9 0.19459265333414777 1108 10-11 0.4212901484169791 1108 12-13 0.5041234165123782 1108 14-15 0.5113089133678628 1108 16-17 0.5571091400967134 1108 18-19 0.5087964851226943 1108 20-21 0.4929080853654426 1108 22-23 0.5539468118956989 1108 24-25 0.5131520270976537 1108 26-27 0.5444790441363452 1108 28-29 0.392483876804711 1108 30-31 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-0.5895797292163927 2316 82-83 -0.6005310003005313 2316 84-85 -0.8667385376525907 2316 86-87 -0.7826506139171947 2316 88-89 -1.1570301894348987 2316 90-91 -1.0467857603226172 2316 92-93 -1.2153923896292405 2316 94-95 -1.4353450951255304 2316 96-97 -1.244418956662372 2316 98-99 -1.2353949685242114 2316 100-101 -0.9666675608510378 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11248.0 3 4107.0 4 4425.0 5 5003.0 6 5677.0 7 7125.0 8 8683.0 9 11128.0 10 13760.0 11 16393.0 12 19414.0 13 22619.0 14 25353.0 15 28839.0 16 32498.0 17 36840.0 18 36497.0 19 35169.0 20 37753.0 21 40560.0 22 44912.0 23 50125.0 24 55604.0 25 62488.0 26 70927.0 27 79745.0 28 90622.0 29 104447.0 30 120084.0 31 139898.0 32 165906.0 33 198616.0 34 241752.0 35 297149.0 36 356056.0 37 360021.0 38 178287.0 39 10681.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.76506027083611 18.296098850584592 14.212168285602736 23.726672592976563 2 15.54331734978613 19.6281349168094 40.27302548607635 24.555522247328117 3 19.64719499818751 23.563560298476517 29.02407475514876 27.765169948187214 4 13.04343950234863 15.949718484660691 36.33791349474229 34.66892851824839 5 14.132069914627445 37.15944750855839 33.732724736908835 14.975757839905329 6 33.2212529057481 35.1898972421809 17.174655272612004 14.414194579459002 7 29.547564980776208 31.897135956959303 20.010272935930484 18.545026126334005 8 26.71720270985337 34.89139229065663 19.33852007781107 19.052884921678924 9 26.636244611546033 16.75331329258719 19.627342479537244 36.98309961632953 10-11 25.372171286936517 26.24856558811218 27.01164300079627 21.367620124155035 12-13 26.209932261652675 23.4079986346363 27.389209038139896 22.99286006557113 14-15 22.909172595077674 25.05072888838126 25.530833624100836 26.509264892440232 16-17 22.750873104422627 28.200728192195207 26.252649021924988 22.795749681457174 18-19 22.736844815752978 26.81796678756899 28.314170199266 22.13101819741203 20-21 23.34022365311086 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22.81004139198237 46-47 22.96104722454111 26.800649424690842 27.3665115930624 22.871791757705648 48-49 22.831502012228317 26.719345922253023 27.41584143221747 23.033310633301195 50-51 22.934316387950815 26.90244070667199 27.29595296861835 22.867289936758844 52-53 23.124393897866437 26.706066639924053 27.28371693468956 22.885822527519945 54-55 22.73457979642034 26.788830081975085 27.4584357375596 23.018154384044973 56-57 22.785545352735433 26.981999426761718 27.238917104364514 22.99353811613834 58-59 22.79000592724407 26.593029349758883 27.346426294257963 23.270538428739084 60-61 22.652389082806653 26.7571366880144 27.547862429991177 23.042611799187775 62-63 22.621449156370936 26.756516926866343 27.651989026832645 22.970044889930076 64-65 22.471781260152962 26.676916776271227 27.55525480647143 23.29604715710438 66-67 22.400626872040192 26.81183130225274 27.421757733228592 23.365784092478474 68-69 22.804003380702998 26.450535767501094 27.57178796088232 23.173672890913586 70-71 22.65374577002641 26.45923258521628 27.35354038784144 23.533481256915866 72-73 22.651699381610978 26.53475071687711 27.418288551617792 23.39526134989412 74-75 22.542345358043082 26.83728140804525 27.269242181551256 23.35113105236041 76-77 22.629309313491643 26.467413661917515 27.351936873659366 23.551340150931477 78-79 22.779641194399456 26.641566642774457 27.27835682294286 23.300435339883233 80-81 22.730939572299018 26.731501246854812 27.157542287708385 23.380016893137785 82-83 22.726079458950093 26.68008467270015 27.220814475606275 23.373021392743485 84-85 22.688589357758566 26.60405256390181 27.192171482626293 23.51518659571333 86-87 22.713276263523056 26.625510267896114 27.08802885403141 23.57318461454942 88-89 22.74645903315212 26.566918781602734 27.008459634240516 23.67816255100463 90-91 22.63419403854943 26.548458139963643 27.164479210320476 23.65286861116645 92-93 22.73898022013567 26.264732166064714 27.186648282144652 23.80963933165496 94-95 22.57755130200098 26.162799142012283 27.358541949037008 23.901107606949726 96-97 22.696341611560644 25.93258799357742 27.309253718336045 24.06181667652589 98-99 22.590666751602196 25.97015462336979 27.057026836216462 24.382151788811548 100-101 23.09322534385057 25.511408492608073 27.215285749613976 24.180080413927378 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 345.0 1 241.5 2 221.5 3 307.5 4 266.0 5 268.5 6 484.0 7 1044.0 8 1676.5 9 1976.0 10 1827.5 11 1630.0 12 1720.5 13 1875.0 14 2040.0 15 2173.0 16 2357.5 17 2581.5 18 2795.5 19 3016.0 20 3411.5 21 4133.5 22 4986.5 23 6040.0 24 7486.0 25 9424.0 26 12348.0 27 16418.5 28 20261.0 29 24141.5 30 28731.0 31 35116.0 32 43486.0 33 48674.0 34 52267.0 35 58000.0 36 64247.5 37 70461.5 38 75475.0 39 79511.5 40 84381.5 41 90867.5 42 96123.5 43 100674.5 44 105832.5 45 111604.0 46 115432.5 47 119304.5 48 123021.0 49 122827.5 50 120570.0 51 116094.5 52 112012.5 53 110894.0 54 110237.0 55 104410.0 56 94929.5 57 86556.5 58 78883.0 59 68931.5 60 57061.0 61 47428.0 62 39562.5 63 30153.5 64 22075.5 65 16827.5 66 12615.0 67 9532.0 68 7229.5 69 5473.5 70 4164.0 71 3047.0 72 2250.5 73 1761.0 74 1355.0 75 1009.5 76 740.5 77 480.5 78 286.0 79 176.0 80 104.5 81 64.5 82 36.5 83 21.5 84 13.0 85 11.0 86 11.0 87 7.5 88 6.0 89 6.5 90 5.0 91 4.5 92 4.0 93 1.5 94 1.0 95 1.5 96 1.0 97 0.5 98 0.5 99 1.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.18096543276444496 2 0.04834324562361632 3 0.04722128633951874 4 0.053656052821843096 5 0.05956283846459212 6 0.06378668518119478 7 0.03610068990596332 8 0.03379077373282124 9 0.04128150189429625 10-11 0.042799446808075334 12-13 0.039054082727337826 14-15 0.035440713856494145 16-17 0.03276781085614403 18-19 0.041116507881928964 20-21 0.050356172574497274 22-23 0.047782265981567526 24-25 0.06728455824338135 26-27 0.05279808395753318 28-29 0.045505348610898916 30-31 0.05345806000700235 32-33 0.05000968514852596 34-35 0.04939920730276698 36-37 0.046891298314784155 38-39 0.027240511441839775 40-41 0.024815099460040593 42-43 0.02130072699661729 44-45 0.0038278610869211553 46-47 0.011434085057053279 48-49 0.010378123377902615 50-51 0.008777681457939892 52-53 0.009652149723486535 54-55 0.01141758565581655 56-57 0.008563189241862413 58-59 0.011120596433555426 60-61 0.011500082662000196 62-63 0.003695865877027323 64-65 0.0066492586984018345 66-67 0.0042733449203128425 68-69 0.009041671877727557 70-71 0.022950667120290206 72-73 0.019964275496442235 74-75 0.02059125274343794 76-77 0.01196206589662861 78-79 0.004520835938863778 80-81 0.006220274266246878 82-83 0.005164312587096214 84-85 0.005230310192043131 86-87 0.008728183254229704 88-89 0.029979412047136812 90-91 0.03740414260366491 92-93 0.022785673107922913 94-95 0.032965803670984783 96-97 0.03633168152327752 98-99 0.02191120484237627 100-101 0.1220460709480853 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3030413.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.630845269925324 #Duplication Level Percentage of deduplicated Percentage of total 1 75.61306858422795 41.30805850209208 2 13.386335413955287 14.626136374622256 3 4.706988462586133 7.714402751606 4 2.0819760742059388 4.549604510625247 5 1.1444748035123324 3.126181295300521 6 0.6581031744094306 2.1571639615684965 7 0.46671345790890506 1.7847865493089237 8 0.33100259688080624 1.4466361323311032 9 0.23516487798025837 1.1562530455674327 >10 1.2468550460318257 12.239582989656899 >50 0.08380730885880752 3.1157438965650366 >100 0.04088993471725649 3.8999140429003334 >500 0.0029180619320392943 1.074631178337943 >1k 0.0015806168792154086 1.3859909237715902 >5k 1.2158591378580067E-4 0.4149138457461535 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7084 0.23376351672197818 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5409 0.1784905225789356 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3907 0.12892632126380135 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 3.299880247345824E-5 8 0.0 0.0 0.0 0.0 3.299880247345824E-5 9 0.0 0.0 0.0 0.0 3.299880247345824E-5 10-11 0.0 0.0 0.0 1.649940123672912E-5 3.299880247345824E-5 12-13 0.0 0.0 0.0 3.299880247345824E-5 1.4849461113056205E-4 14-15 0.0 0.0 0.0 3.299880247345824E-5 1.9799281484074942E-4 16-17 0.0 0.0 0.0 3.299880247345824E-5 2.3099161731420767E-4 18-19 0.0 0.0 0.0 3.299880247345824E-5 2.3099161731420767E-4 20-21 0.0 0.0 0.0 4.9498203710187356E-5 2.3099161731420767E-4 22-23 0.0 0.0 0.0 1.9799281484074942E-4 2.3099161731420767E-4 24-25 0.0 0.0 0.0 4.454838333916862E-4 2.3099161731420767E-4 26-27 0.0 0.0 0.0 9.899640742037472E-4 2.8048982102439504E-4 28-29 0.0 0.0 0.0 0.0037783628832109683 2.9698922226112416E-4 30-31 0.0 0.0 0.0 0.013925494643799375 2.9698922226112416E-4 32-33 0.0 0.0 0.0 0.03418675936250273 2.9698922226112416E-4 34-35 0.0 0.0 0.0 0.06512313668136983 2.9698922226112416E-4 36-37 0.0 0.0 0.0 0.1166177679412014 2.9698922226112416E-4 38-39 0.0 0.0 0.0 0.19200353219181676 2.9698922226112416E-4 40-41 0.0 0.0 0.0 0.27339507849260153 2.9698922226112416E-4 42-43 1.649940123672912E-5 0.0 0.0 0.3632343182265916 2.9698922226112416E-4 44-45 3.299880247345824E-5 0.0 0.0 0.4690779771602088 2.9698922226112416E-4 46-47 6.599760494691648E-5 0.0 0.0 0.5986807738747161 3.134886234978533E-4 48-49 6.599760494691648E-5 0.0 0.0 0.7359062939605922 3.2998802473458236E-4 50-51 6.599760494691648E-5 0.0 0.0 0.8847308931158888 3.2998802473458236E-4 52-53 6.599760494691648E-5 0.0 0.0 1.0311960778943332 3.2998802473458236E-4 54-55 6.599760494691648E-5 0.0 0.0 1.1905142962361897 3.2998802473458236E-4 56-57 6.599760494691648E-5 0.0 0.0 1.3798449254276561 3.464874259713115E-4 58-59 6.599760494691648E-5 0.0 0.0 1.582441073213453 3.629868272080406E-4 60-61 6.599760494691648E-5 0.0 0.0 1.803483551581913 3.9598562968149885E-4 62-63 6.599760494691648E-5 0.0 0.0 2.0236515616848263 3.9598562968149885E-4 64-65 6.599760494691648E-5 0.0 0.0 2.255204158641083 4.289844321549571E-4 66-67 6.599760494691648E-5 0.0 0.0 2.521751985620442 4.454838333916862E-4 68-69 6.599760494691648E-5 0.0 0.0 2.818394720455595 4.949820371018736E-4 70-71 6.599760494691648E-5 0.0 0.0 3.1360081942626303 5.114814383386027E-4 72-73 8.249700618364559E-5 0.0 0.0 3.4591159686814965 5.279808395753318E-4 74-75 9.899640742037471E-5 0.0 0.0 3.784748151489582 5.279808395753318E-4 76-77 9.899640742037471E-5 0.0 0.0 4.134106473276085 5.279808395753318E-4 78-79 9.899640742037471E-5 0.0 0.0 4.5245978023457525 5.279808395753318E-4 80-81 1.6499401236729118E-4 0.0 0.0 4.94026721770267 5.774790432855191E-4 82-83 1.6499401236729118E-4 0.0 0.0 5.374250968432355 6.599760494691647E-4 84-85 1.6499401236729118E-4 0.0 0.0 5.80889469521151 6.599760494691647E-4 86-87 1.6499401236729118E-4 0.0 0.0 6.270811932234979 6.764754507058938E-4 88-89 1.6499401236729118E-4 0.0 0.0 6.757313277101174 6.92974851942623E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5335 0.0 45.779144 1 GTATCAA 9180 0.0 39.518192 1 ATCAACG 11485 0.0 31.199654 3 TATCAAC 11595 0.0 31.026625 2 TCAACGC 11675 0.0 30.529085 4 CAACGCA 11870 0.0 29.985535 5 AACGCAG 12385 0.0 28.811117 6 ACGCAGA 14330 0.0 24.56539 7 CGCAGAG 14495 0.0 24.286156 8 GTACATG 11945 0.0 23.515303 1 TACATGG 11675 0.0 23.1614 2 ACATGGG 11975 0.0 22.06524 3 GCAGAGT 15800 0.0 21.13662 9 TGGTATC 2860 0.0 20.77081 2 GAGTACT 7750 0.0 20.469816 12-13 GGACCGA 970 0.0 20.082985 6 ACCGAGT 1045 0.0 20.003002 8 AGAGTAC 13930 0.0 19.625015 10-11 GTGGTAT 3180 0.0 19.312893 1 AGTACTT 8445 0.0 18.95394 12-13 >>END_MODULE