FastQCFastQC Report
Thu 26 May 2016
SRR938454_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938454_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3030413
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69100.22802172509159643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58670.1936039741117795No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40200.1326551859433021No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA52900.045.6665531
GTATCAA87200.038.2288931
TATCAAC111700.029.715652
ATCAACG110850.029.7152733
TCAACGC113500.029.0214794
CAACGCA114650.028.6889215
AACGCAG119200.027.652126
TAAGGTG14750.024.1685965
TACATGG114950.023.787122
GTACATG120500.023.5206551
ACGCAGA140050.023.3997527
CGCAGAG141450.023.0338428
GTAAGGT13950.022.8287854
ACATGGG118650.021.7928163
GCAGAGT157150.020.279329
GAGTACT79550.020.26958512-13
GTACTTT82300.019.27488914-15
TGGTATC27750.019.1927872
AGAGTAC138700.019.14170510-11
AGTACTT85800.018.73771712-13
Produced by FastQC (version 0.11.5)