##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938454_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3030413 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.77303390659953 31.0 31.0 34.0 30.0 34.0 2 32.003404816439215 31.0 31.0 34.0 30.0 34.0 3 32.07314250565847 33.0 31.0 34.0 30.0 34.0 4 35.74665697381842 37.0 35.0 37.0 33.0 37.0 5 35.611088983580785 37.0 35.0 37.0 33.0 37.0 6 35.63670628392896 37.0 35.0 37.0 33.0 37.0 7 35.57183921795478 37.0 35.0 37.0 33.0 37.0 8 35.55857369936045 37.0 35.0 37.0 33.0 37.0 9 37.13513735586535 39.0 37.0 39.0 33.0 39.0 10-11 37.04419876102696 39.0 37.0 39.0 33.0 39.0 12-13 36.938746137902655 39.0 37.0 39.0 33.0 39.0 14-15 37.99981603167621 40.0 37.0 41.0 33.0 41.0 16-17 37.92277785239174 40.0 37.0 41.0 33.0 41.0 18-19 38.021191995942466 40.0 37.0 41.0 33.0 41.0 20-21 38.070136809735175 40.0 37.0 41.0 33.0 41.0 22-23 37.97598330656581 40.0 37.0 41.0 33.0 41.0 24-25 37.885141233224644 40.0 37.0 41.0 32.0 41.0 26-27 37.70692707561642 40.0 37.0 41.0 32.0 41.0 28-29 37.591102103904646 39.5 37.0 41.0 32.0 41.0 30-31 37.36817159905267 39.0 36.0 41.0 31.5 41.0 32-33 37.17539902976921 39.0 36.0 41.0 31.0 41.0 34-35 37.03905111283511 39.0 36.0 41.0 31.0 41.0 36-37 37.0586317772528 39.0 36.0 41.0 31.0 41.0 38-39 36.94916583977167 39.0 36.0 41.0 31.0 41.0 40-41 36.785232573909894 39.0 35.0 41.0 30.0 41.0 42-43 36.586872812385636 39.0 35.0 40.5 30.0 41.0 44-45 36.3447396443983 39.0 35.0 40.0 30.0 41.0 46-47 36.01147153869786 38.5 35.0 40.0 29.5 41.0 48-49 35.68487397592341 38.0 35.0 40.0 28.0 41.0 50-51 35.34269883345934 38.0 34.0 40.0 27.0 41.0 52-53 34.856667061552336 38.0 34.0 40.0 26.0 41.0 54-55 34.55497798484893 38.0 33.0 40.0 26.0 41.0 56-57 34.227180090634505 37.0 33.0 40.0 25.0 41.0 58-59 33.79392940830177 37.0 32.5 40.0 23.5 41.0 60-61 33.43215017227025 36.5 32.0 39.0 23.0 40.5 62-63 32.97782744464203 36.0 31.5 39.0 22.0 40.0 64-65 32.48620023079363 35.0 31.0 39.0 21.0 40.0 66-67 31.99885708647633 35.0 31.0 38.0 19.5 40.0 68-69 31.47102886636244 35.0 30.0 38.0 18.0 40.0 70-71 30.90071683298613 34.0 29.0 37.0 16.0 39.0 72-73 30.461698619957083 34.0 29.0 36.5 13.0 39.0 74-75 29.94236000175554 34.0 29.0 36.0 9.0 39.0 76-77 28.675892031878163 32.5 27.0 34.5 8.0 37.0 78-79 29.38777140277579 33.5 29.0 35.0 7.0 37.0 80-81 29.119851320595576 34.0 29.0 35.0 6.0 37.0 82-83 28.65542254471585 33.0 27.5 35.0 2.0 36.0 84-85 28.138906644077885 33.0 27.0 35.0 2.0 36.0 86-87 27.578983128702262 32.5 26.0 35.0 2.0 35.5 88-89 26.95125548893831 32.0 25.0 34.5 2.0 35.0 90-91 26.42535208897269 32.0 25.0 34.0 2.0 35.0 92-93 25.693696700746727 31.5 23.5 34.0 2.0 35.0 94-95 24.939939539594107 31.0 19.5 34.0 2.0 35.0 96-97 23.95220271956331 31.0 9.5 34.0 2.0 35.0 98-99 22.727475924898684 30.5 2.0 34.0 2.0 35.0 100-101 19.071510384888132 25.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.28295411585967045 1101 2 0.34165781890312275 1101 3 0.3060961815463443 1101 4 0.2240083067492904 1101 5 0.13747399399133542 1101 6 0.1561076685005034 1101 7 0.2405060672263275 1101 8 0.2952355801784634 1101 9 0.307241328591104 1101 10-11 0.34408323088180737 1101 12-13 0.3315274369633272 1101 14-15 0.40102166208632894 1101 16-17 0.4480053566770934 1101 18-19 0.5418608623726442 1101 20-21 0.4350436685116037 1101 22-23 0.47504059600115767 1101 24-25 0.35138225459984795 1101 26-27 0.5338853135388035 1101 28-29 0.42244768487946516 1101 30-31 0.33226127086791024 1101 32-33 0.43273989616717046 1101 34-35 0.34003640968459337 1101 36-37 0.41142394518273306 1101 38-39 0.47154789463701974 1101 40-41 0.45372799835304534 1101 42-43 0.6389130709507 1101 44-45 0.5643860825184817 1101 46-47 0.5527689444636721 1101 48-49 0.5990362314598983 1101 50-51 0.569730914803273 1101 52-53 0.6321048050439728 1101 54-55 0.4723326495505731 1101 56-57 0.4586580135964766 1101 58-59 0.5614806273495603 1101 60-61 0.5288768398479036 1101 62-63 0.5648778485485337 1101 64-65 0.535067515018973 1101 66-67 0.44579002785738453 1101 68-69 0.4938920140672849 1101 70-71 0.3947459110958036 1101 72-73 0.38927743214781785 1101 74-75 0.4878615425591306 1101 76-77 0.4144187740591221 1101 78-79 0.5344807701481216 1101 80-81 0.5451947568198392 1101 82-83 0.4616388809834042 1101 84-85 0.5150125779854662 1101 86-87 0.5552249017213278 1101 88-89 0.5220663063258328 1101 90-91 0.5082203916920314 1101 92-93 0.5498111389925526 1101 94-95 0.5986207095747211 1101 96-97 0.6114884659590736 1101 98-99 0.525914107746793 1101 100-101 0.38951176372363605 1102 1 0.2894636945749589 1102 2 0.2829387796547209 1102 3 0.2668225798699453 1102 4 0.17457811368529264 1102 5 0.19729950147952025 1102 6 0.2745322950845477 1102 7 0.24954948676312938 1102 8 0.2885107410651244 1102 9 0.23406868329654174 1102 10-11 0.31536229227695145 1102 12-13 0.3707503891343009 1102 14-15 0.42927142373572025 1102 16-17 0.39960094023884096 1102 18-19 0.31135881156424006 1102 20-21 0.3441801316864357 1102 22-23 0.38718722880216916 1102 24-25 0.38099067681809373 1102 26-27 0.2964623429949711 1102 28-29 0.30458529263379575 1102 30-31 0.296477581498344 1102 32-33 0.41560894236746293 1102 34-35 0.44448189691296847 1102 36-37 0.46963468933320485 1102 38-39 0.5026722640010135 1102 40-41 0.547482901920354 1102 42-43 0.550286678089293 1102 44-45 0.5758589403423429 1102 46-47 0.5133112417663384 1102 48-49 0.6955719542581349 1102 50-51 0.6761762505797009 1102 52-53 0.5495983124725683 1102 54-55 0.5427888426940157 1102 56-57 0.5903513829803373 1102 58-59 0.724643788275948 1102 60-61 0.5059730838394785 1102 62-63 0.35603032342240937 1102 64-65 0.4050579934085121 1102 66-67 0.5647296531503585 1102 68-69 0.45882002473347683 1102 70-71 0.22108141937848913 1102 72-73 0.17843589309804386 1102 74-75 0.2639583898757003 1102 76-77 0.152683171925883 1102 78-79 0.3168791359567287 1102 80-81 0.5090118301722413 1102 82-83 0.44623423116017946 1102 84-85 0.3044839361867133 1102 86-87 0.2111939707635564 1102 88-89 0.17946305830216147 1102 90-91 0.17812800600101397 1102 92-93 0.40724060210779456 1102 94-95 0.4641029474883567 1102 96-97 0.21236260442091748 1102 98-99 0.02568097105271505 1102 100-101 0.0030941486201534474 1103 1 0.26977290215173255 1103 2 0.28077021692588744 1103 3 0.30492899285656705 1103 4 0.1626875622394266 1103 5 0.18699818637826837 1103 6 0.2480334252496803 1103 7 0.32728616762691587 1103 8 0.3472889017914227 1103 9 0.3720216833712584 1103 10-11 0.3490366599448933 1103 12-13 0.43691992324474427 1103 14-15 0.5838373842826812 1103 16-17 0.5227854373620602 1103 18-19 0.4986643017383443 1103 20-21 0.4296531898572411 1103 22-23 0.39698405593360775 1103 24-25 0.43974723831401974 1103 26-27 0.4837247514609402 1103 28-29 0.486285957656591 1103 30-31 0.5420772928859279 1103 32-33 0.5833861597327754 1103 34-35 0.6200764011347459 1103 36-37 0.5570231906034735 1103 38-39 0.5379647059049262 1103 40-41 0.6374467319659374 1103 42-43 0.5933386143143551 1103 44-45 0.6254652505866858 1103 46-47 0.6045431217633492 1103 48-49 0.7333227388355752 1103 50-51 0.7351852311894262 1103 52-53 0.8256982386849288 1103 54-55 0.8689092006107799 1103 56-57 0.7764240824871749 1103 58-59 0.8226884157884697 1103 60-61 0.8321420108364208 1103 62-63 0.7449196793216828 1103 64-65 0.6361740781802396 1103 66-67 0.6969878118218062 1103 68-69 0.6791024025635579 1103 70-71 0.4362027486768483 1103 72-73 0.4760451501779208 1103 74-75 0.6837039392667172 1103 76-77 0.6054869855326963 1103 78-79 0.6238391084965151 1103 80-81 0.5849971053347609 1103 82-83 0.5429310666978004 1103 84-85 0.43242703564124696 1103 86-87 0.6285665236291322 1103 88-89 0.8122522077828407 1103 90-91 0.9119000955922871 1103 92-93 0.7100097401934242 1103 94-95 0.8091206471820023 1103 96-97 0.8273516530273604 1103 98-99 0.6488638336395809 1103 100-101 0.4954125282809123 1104 1 0.32590759372815015 1104 2 0.27564940412776195 1104 3 0.3029548840233218 1104 4 0.15949027138901783 1104 5 0.11907220209170788 1104 6 0.13766659711174611 1104 7 0.1294589409220066 1104 8 0.2621755794023102 1104 9 0.31101156976598787 1104 10-11 0.39250410695124316 1104 12-13 0.3186470083285542 1104 14-15 0.5307636430252884 1104 16-17 0.4617440952298608 1104 18-19 0.41187695280842007 1104 20-21 0.44631175078903595 1104 22-23 0.3895598758016945 1104 24-25 0.4462561397199565 1104 26-27 0.507900457020412 1104 28-29 0.5052653028981027 1104 30-31 0.474983884423672 1104 32-33 0.5407129601100493 1104 34-35 0.46927683990941915 1104 36-37 0.624272242487649 1104 38-39 0.5876467407047059 1104 40-41 0.6098330041051838 1104 42-43 0.6106206918911212 1104 44-45 0.4369272071409753 1104 46-47 0.51766646923069 1104 48-49 0.4076478642988164 1104 50-51 0.5715162442561521 1104 52-53 0.5958677069701679 1104 54-55 0.6380532860387191 1104 56-57 0.5329179520444569 1104 58-59 0.5628556453821503 1104 60-61 0.5507023169491987 1104 62-63 0.6022804150679946 1104 64-65 0.6158451946438959 1104 66-67 0.5822128247292326 1104 68-69 0.4764151184869121 1104 70-71 0.4310953036850975 1104 72-73 0.09640089135677599 1104 74-75 0.22891557993032308 1104 76-77 0.3072576041814621 1104 78-79 0.3926309161722763 1104 80-81 0.47587731048677284 1104 82-83 0.38739203569846836 1104 84-85 0.5446104987439924 1104 86-87 0.5271740974177597 1104 88-89 0.38647869991292083 1104 90-91 0.33220245429457407 1104 92-93 0.28689287583483747 1104 94-95 0.49721988267659967 1104 96-97 0.466320261868038 1104 98-99 0.24237169468627684 1104 100-101 -0.18255701619527542 1105 1 0.2814643281347564 1105 2 0.29729823272636224 1105 3 0.2320968753155057 1105 4 0.19532989304472181 1105 5 0.16914563182209008 1105 6 0.17630817218292805 1105 7 0.1788082667336539 1105 8 0.20083381612174378 1105 9 0.2961997807479193 1105 10-11 0.30481222981963896 1105 12-13 0.3262433750432123 1105 14-15 0.4350132278983452 1105 16-17 0.3217410468206765 1105 18-19 0.3363586992309422 1105 20-21 0.41491927354990565 1105 22-23 0.36409391802536817 1105 24-25 0.48178627739683577 1105 26-27 0.35463666854585085 1105 28-29 0.4114852530924793 1105 30-31 0.48707814900615887 1105 32-33 0.5282284649958839 1105 34-35 0.4551942248183565 1105 36-37 0.4726771805262757 1105 38-39 0.5050229505821306 1105 40-41 0.5081461963414355 1105 42-43 0.4295421442275469 1105 44-45 0.4162092854302344 1105 46-47 0.23281702019115613 1105 48-49 0.23364283207545355 1105 50-51 0.18442415444036442 1105 52-53 0.26655305788038675 1105 54-55 0.4408115519934128 1105 56-57 0.49427018622632346 1105 58-59 0.4160311943653099 1105 60-61 0.4182288712509603 1105 62-63 0.5345083869760927 1105 64-65 0.3185872472458229 1105 66-67 0.3176958700345054 1105 68-69 0.29713674397212486 1105 70-71 0.21944022990366818 1105 72-73 0.2846002389587845 1105 74-75 0.252016405530469 1105 76-77 0.2529286763473202 1105 78-79 0.3098053397203415 1105 80-81 0.4331991549323 1105 82-83 0.40785106677612504 1105 84-85 0.49592368959266153 1105 86-87 0.5119887288425637 1105 88-89 0.50395885029684 1105 90-91 0.6675663170474202 1105 92-93 0.5049965198259017 1105 94-95 0.44061484133788653 1105 96-97 0.3624508422260142 1105 98-99 0.16056113389106486 1105 100-101 0.03306680669556883 1106 1 0.15760080935226384 1106 2 0.10984027789422157 1106 3 0.11298067544510104 1106 4 0.08398884547229102 1106 5 0.0651318184420191 1106 6 -0.005188497866200237 1106 7 0.06469885697620725 1106 8 0.06785878908864618 1106 9 0.03711688831305793 1106 10-11 -0.02705740762140607 1106 12-13 -0.06578938998653427 1106 14-15 0.08230488230046262 1106 16-17 -0.0922005997444586 1106 18-19 0.13689705705409239 1106 20-21 0.16396380123886445 1106 22-23 0.20992654102766295 1106 24-25 0.1271322677106852 1106 26-27 0.04698272259132352 1106 28-29 0.20317870800277404 1106 30-31 0.19441926041590563 1106 32-33 0.17325304923159024 1106 34-35 0.10435861657611412 1106 36-37 -0.29333317020797267 1106 38-39 0.005992030598712006 1106 40-41 0.31976212485040634 1106 42-43 0.41996179106980236 1106 44-45 0.38718217349055095 1106 46-47 0.09052343640135518 1106 48-49 0.127208538163174 1106 50-51 0.19778190096628379 1106 52-53 0.1927459199174848 1106 54-55 -0.12430773975906817 1106 56-57 0.19504466812680477 1106 58-59 0.31770138564790784 1106 60-61 -0.14359291674129793 1106 62-63 0.01579179389320018 1106 64-65 -0.47061745079998474 1106 66-67 -0.15849640541800625 1106 68-69 -0.8427860340163917 1106 70-71 -0.5123424344819085 1106 72-73 -0.15666605025743152 1106 74-75 0.03386570275457146 1106 76-77 -0.01713486820261423 1106 78-79 -0.04807699902094953 1106 80-81 -0.7523027252562642 1106 82-83 -0.12579991708476612 1106 84-85 -0.019827744818041282 1106 86-87 0.048255774277293995 1106 88-89 -0.11913529145645185 1106 90-91 -0.10203313868663244 1106 92-93 -0.13434881583841118 1106 94-95 0.06803559930875025 1106 96-97 0.04912647289163985 1106 98-99 -0.26013997526112576 1106 100-101 -0.5366090847095499 1107 1 0.13487303293341668 1107 2 0.17277162443417637 1107 3 0.18401444195816197 1107 4 0.11826529961494003 1107 5 0.16342875887762176 1107 6 0.1820368316562906 1107 7 0.06969884466374765 1107 8 0.20862694709527574 1107 9 0.11964936448023877 1107 10-11 0.2363430641920914 1107 12-13 0.16692745501928385 1107 14-15 0.318519748693852 1107 16-17 0.30868629758111155 1107 18-19 0.2807875524910983 1107 20-21 0.2974700186211052 1107 22-23 0.26112898620026925 1107 24-25 0.24565165532172273 1107 26-27 0.17985936281630188 1107 28-29 0.29224581748025713 1107 30-31 0.2408059943961689 1107 32-33 0.3128067368956593 1107 34-35 0.1969386249978342 1107 36-37 0.1509588448329282 1107 38-39 0.18269389995858631 1107 40-41 0.2066528837740691 1107 42-43 0.31911781293016617 1107 44-45 0.37657083313965956 1107 46-47 0.3493176627601926 1107 48-49 0.3269580514105641 1107 50-51 0.3263044959733321 1107 52-53 0.493425957642863 1107 54-55 0.3019841246594055 1107 56-57 0.2454105328965852 1107 58-59 0.2487294945852767 1107 60-61 0.09948927591095469 1107 62-63 0.24408867071441875 1107 64-65 0.3585659847541294 1107 66-67 0.32472668693036866 1107 68-69 0.3723852466765223 1107 70-71 0.2200868632645836 1107 72-73 0.4620091412834135 1107 74-75 0.4601363281783222 1107 76-77 0.4095429918588813 1107 78-79 0.4925255974307632 1107 80-81 0.511935301696532 1107 82-83 0.508594644337176 1107 84-85 0.4934635565105552 1107 86-87 0.6674875916457417 1107 88-89 0.5703541456835097 1107 90-91 0.7058377352025786 1107 92-93 0.4784455853573739 1107 94-95 0.22051172749952386 1107 96-97 0.34351751089774396 1107 98-99 0.20047159081821064 1107 100-101 -0.3775922695819709 1108 1 0.23273985615969295 1108 2 0.22210099530845895 1108 3 0.15976340300483827 1108 4 0.1478629612272968 1108 5 0.12253199128107894 1108 6 0.10748469949579942 1108 7 0.09756511284347624 1108 8 0.21074355064820338 1108 9 0.15367215422234892 1108 10-11 0.1771977733320611 1108 12-13 0.20155463963558162 1108 14-15 0.26877956520014834 1108 16-17 0.24178120194111585 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78428.0 29 93405.0 30 111350.0 31 133139.0 32 160010.0 33 196988.0 34 249786.0 35 326380.0 36 432686.0 37 502730.0 38 260047.0 39 12575.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.48740756971375 19.196790382234376 14.82722367271297 23.4885783753389 2 16.037940790972392 20.35847642566847 39.291862142736875 24.311720640622266 3 19.653028501583787 23.77265918974866 29.552734579310343 27.021577729357215 4 13.542246551872633 16.70445579529919 35.970212640983256 33.78308501184492 5 14.082245819292504 37.25277654199765 33.93783624091206 14.72714139779779 6 33.26790770762929 35.694606642724935 16.793552561977528 14.243933087668248 7 29.446745377610245 32.02131194658946 20.017304572017082 18.514638103783213 8 26.700750029781418 35.33442471372714 19.15547484781777 18.80935040867367 9 26.332087408547945 16.729831874401278 19.567761885921158 37.370318831129616 10-11 25.38266236318284 26.439877996827494 26.972214678329326 21.20524496166034 12-13 26.136652000898884 23.4548228244797 27.467163716628722 22.94136145799269 14-15 22.73952428266378 25.17392843813698 25.546187928840062 26.54035935035918 16-17 22.665953452549207 28.376858203815786 26.29255484318474 22.664633500450268 18-19 22.63371362253264 26.95111194414755 28.397119468534488 22.01805496478533 20-21 23.220547826319383 26.133500615262673 28.47643209027944 22.169519468138503 22-23 22.921661172916036 26.08387041634259 28.350987142676594 22.643481268064782 24-25 22.784827252576964 26.29664926196382 28.420225402066457 22.498298083392758 26-27 22.360856311500484 26.63888264609217 28.30308574566549 22.697175296741854 28-29 22.513515484523065 26.82114616060583 28.291638796428074 22.373699558443025 30-31 22.645428197410716 26.45033861721158 28.182808745870613 22.72142443950709 32-33 22.378352389591782 26.486472305920017 28.13847485474752 22.99670044974068 34-35 22.94098340800345 26.557540653287486 28.037522125879775 22.463953812829292 36-37 23.055509357155866 26.170848941792656 28.079519325450562 22.69412237560091 38-39 22.613358431053676 26.49764205619366 27.993308227912955 22.89569128483971 40-41 23.014160003735654 26.337438645951394 28.07303200500419 22.57536934530876 42-43 22.75638905035458 26.29598836721033 27.919437684701307 23.02818489773378 44-45 22.699071496042205 26.568660207150018 28.032718318050758 22.699549978757016 46-47 22.953488517901686 26.815585862389053 27.463055365720777 22.76787025398848 48-49 22.789236978590047 26.730267458593925 27.538177139551607 22.942318423264418 50-51 22.917173335779644 26.890641638614937 27.45129129263899 22.74089373296643 52-53 23.167403254935877 26.66976415425884 27.35085943731102 22.81197315349426 54-55 22.765345845599263 26.781531098236442 27.503330404139636 22.94979265202466 56-57 22.803277969042504 26.95916365195107 27.296460911433524 22.941097467572902 58-59 22.82207078705114 26.676809398586926 27.325598854017585 23.175520960344347 60-61 22.61912815183937 26.813655432444357 27.57643925101958 22.990777164696695 62-63 22.61407933506093 26.840285466040438 27.624452508618464 22.92118269028017 64-65 22.424649709462045 26.749654255047083 27.51776738022177 23.3079286552691 66-67 22.383427058290305 26.863960134760667 27.380012503260698 23.37260030368833 68-69 22.742589585697242 26.551236717633316 27.536657525350368 23.169516171319078 70-71 22.630038595463052 26.5594104820209 27.285814319391978 23.52473660312407 72-73 22.656965017138756 26.64990327224429 27.222317093794985 23.47081461682197 74-75 22.493221221001892 26.981454343021895 27.053012246185588 23.47231218979063 76-77 22.55445049899139 26.565339443831583 27.296906395266916 23.58330366191011 78-79 22.63793746924924 26.704610889670814 27.251516542464678 23.40593509861527 80-81 22.635859391925038 26.76930331694203 27.143746474230095 23.451090816902838 82-83 22.60709763664005 26.779499265605832 27.13110429057128 23.48229880718284 84-85 22.555665832576384 26.727288777566848 27.077012686257472 23.640032703599292 86-87 22.581113010421273 26.704132209587044 26.959912089072514 23.75484269091917 88-89 22.47736734016728 26.748636859593773 26.88297509528825 23.891020704950698 90-91 22.39903587389933 26.717687866154904 27.029652751584077 23.853623508361686 92-93 22.396285094327077 26.50950629564909 27.07961509200229 24.014593518021545 94-95 22.240811559884122 26.378088648701446 27.192070646522865 24.189029144891567 96-97 22.269621335441737 26.167439883606626 27.210812519613665 24.352126261337975 98-99 22.052560544612774 26.281988245814926 26.964008170511583 24.70144303906072 100-101 22.203231408265374 26.09732173425235 27.183889981974403 24.515556875507873 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 377.0 1 267.5 2 185.5 3 243.0 4 234.0 5 246.0 6 455.5 7 1018.0 8 1689.0 9 1956.0 10 1742.5 11 1506.0 12 1570.5 13 1745.0 14 1929.5 15 2109.0 16 2241.5 17 2466.0 18 2731.0 19 3014.5 20 3458.5 21 4010.5 22 4684.5 23 5824.5 24 7280.5 25 9109.5 26 12130.5 27 16114.5 28 20064.0 29 24261.0 30 28973.5 31 35278.5 32 44126.5 33 49534.5 34 52581.5 35 58084.5 36 64284.0 37 70551.0 38 75881.0 39 79968.0 40 84433.5 41 91229.0 42 96852.5 43 101722.5 44 107309.5 45 113035.0 46 116834.0 47 120968.5 48 124634.0 49 124407.0 50 121998.0 51 117506.5 52 112895.0 53 111805.0 54 112716.0 55 106438.5 56 95930.5 57 86594.0 58 78438.5 59 68191.0 60 55374.0 61 45467.5 62 37681.5 63 28093.0 64 19712.5 65 14666.5 66 10691.5 67 8095.5 68 6124.5 69 4510.0 70 3373.0 71 2360.5 72 1761.5 73 1452.0 74 1132.5 75 829.5 76 577.0 77 354.0 78 203.5 79 114.5 80 65.5 81 39.0 82 26.5 83 15.5 84 7.0 85 3.5 86 1.5 87 0.5 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.92974851942623E-4 2 0.04893722406813857 3 6.599760494691647E-4 4 0.0 5 0.08698484332003592 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.9799281484074942E-4 26-27 0.008381695828258392 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010147131760588408 36-37 1.6499401236729118E-4 38-39 0.0041083509079455505 40-41 0.005032317377202382 42-43 4.4548383339168627E-4 44-45 1.649940123672912E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.1549580865710385E-4 68-69 0.0010724610803873927 70-71 1.6499401236729118E-4 72-73 1.649940123672912E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001534444315015808 82-83 7.919712593629977E-4 84-85 0.005461301809357339 86-87 0.0024089125805624514 88-89 8.249700618364559E-5 90-91 0.0034318754572396568 92-93 8.744682655466433E-4 94-95 6.599760494691648E-5 96-97 0.0 98-99 9.899640742037471E-5 100-101 1.649940123672912E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3030413.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.655583221440814 #Duplication Level Percentage of deduplicated Percentage of total 1 73.43231687279946 37.93189155366105 2 14.230450129999772 14.70164401937533 3 5.1219017490273675 7.937244661467793 4 2.3888153290560368 4.935825961228305 5 1.2970928617562996 3.350104413319467 6 0.7839762230235368 2.4298049419213887 7 0.52901360010258 1.9128554231760981 8 0.37230730268543266 1.5385400686254018 9 0.278961428108411 1.2968923738703404 >10 1.4169806258527085 13.154108304983552 >50 0.09543032613295495 3.3666763632163725 >100 0.0476372869661332 4.369571031963601 >500 0.0033255719182630557 1.1799933551483848 >1k 0.001662785958795391 1.4727535417497426 >5k 1.279066122150301E-4 0.42209398629318456 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6910 0.22802172509159643 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5867 0.1936039741117795 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4020 0.1326551859433021 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.299880247345824E-5 2 0.0 0.0 0.0 0.0 3.299880247345824E-5 3 0.0 0.0 0.0 0.0 3.299880247345824E-5 4 0.0 0.0 0.0 0.0 3.299880247345824E-5 5 0.0 0.0 0.0 0.0 3.299880247345824E-5 6 0.0 0.0 0.0 0.0 3.299880247345824E-5 7 0.0 0.0 0.0 0.0 3.299880247345824E-5 8 0.0 0.0 0.0 0.0 3.299880247345824E-5 9 0.0 0.0 0.0 0.0 3.299880247345824E-5 10-11 0.0 0.0 0.0 1.649940123672912E-5 3.299880247345824E-5 12-13 0.0 0.0 0.0 3.299880247345824E-5 6.599760494691648E-5 14-15 0.0 0.0 0.0 3.299880247345824E-5 8.249700618364559E-5 16-17 0.0 0.0 0.0 3.299880247345824E-5 9.899640742037471E-5 18-19 1.649940123672912E-5 0.0 0.0 3.299880247345824E-5 1.3199520989383296E-4 20-21 3.299880247345824E-5 0.0 0.0 4.9498203710187356E-5 1.3199520989383296E-4 22-23 3.299880247345824E-5 0.0 0.0 1.9799281484074942E-4 1.3199520989383296E-4 24-25 3.299880247345824E-5 0.0 0.0 4.454838333916862E-4 1.3199520989383296E-4 26-27 3.299880247345824E-5 0.0 0.0 9.899640742037472E-4 1.3199520989383296E-4 28-29 3.299880247345824E-5 0.0 0.0 0.003728864679500781 1.3199520989383296E-4 30-31 3.299880247345824E-5 0.0 0.0 0.013991492248746292 1.3199520989383296E-4 32-33 3.299880247345824E-5 0.0 0.0 0.03441775097981695 1.3199520989383296E-4 34-35 3.299880247345824E-5 0.0 0.0 0.06560161931723497 1.9799281484074942E-4 36-37 6.599760494691648E-5 0.0 0.0 0.11709625057706655 1.9799281484074942E-4 38-39 6.599760494691648E-5 0.0 0.0 0.19279550345117974 1.9799281484074942E-4 40-41 6.599760494691648E-5 0.0 0.0 0.27461603418411945 1.9799281484074942E-4 42-43 6.599760494691648E-5 0.0 0.0 0.3652307457762358 1.9799281484074942E-4 44-45 9.899640742037471E-5 0.0 0.0 0.4713878933333509 2.474910185509368E-4 46-47 1.3199520989383296E-4 0.0 0.0 0.6015021714861968 2.639904197876659E-4 48-49 1.3199520989383296E-4 0.0 0.0 0.7392226736091747 2.639904197876659E-4 50-51 1.3199520989383296E-4 0.0 0.0 0.888014273961998 2.9698922226112416E-4 52-53 1.3199520989383296E-4 0.0 0.0 1.0348754443701238 2.9698922226112416E-4 54-55 1.6499401236729118E-4 0.0 0.0 1.1948371393602126 2.9698922226112416E-4 56-57 1.6499401236729118E-4 0.0 0.0 1.385042236817226 2.9698922226112416E-4 58-59 1.6499401236729118E-4 0.0 0.0 1.5876383846030229 2.9698922226112416E-4 60-61 1.6499401236729118E-4 0.0 0.0 1.8085488677615889 2.9698922226112416E-4 62-63 1.6499401236729118E-4 0.0 0.0 2.0292118599016042 2.9698922226112416E-4 64-65 1.6499401236729118E-4 0.0 0.0 2.2613749347036194 2.9698922226112416E-4 66-67 1.6499401236729118E-4 0.0 0.0 2.5298366922264393 3.134886234978533E-4 68-69 1.6499401236729118E-4 0.0 0.0 2.8278983755679508 3.2998802473458236E-4 70-71 1.6499401236729118E-4 0.0 0.0 3.147607273332051 3.2998802473458236E-4 72-73 1.6499401236729118E-4 0.0 0.0 3.474311917220524 3.629868272080406E-4 74-75 1.6499401236729118E-4 0.0 0.0 3.8050424150107593 4.289844321549571E-4 76-77 1.6499401236729118E-4 0.0 0.0 4.1605385140573246 4.6198323462841534E-4 78-79 1.6499401236729118E-4 0.0 0.0 4.558735063504545 4.6198323462841534E-4 80-81 1.6499401236729118E-4 0.0 0.0 4.982885171097141 4.7848263586514446E-4 82-83 1.6499401236729118E-4 0.0 0.0 5.425333114661269 5.609796420487901E-4 84-85 1.6499401236729118E-4 0.0 0.0 5.869892981583698 5.609796420487901E-4 86-87 1.6499401236729118E-4 0.0 0.0 6.343673288096375 5.774790432855191E-4 88-89 1.6499401236729118E-4 0.0 0.0 6.844529112038524 5.939784445222483E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5290 0.0 45.666553 1 GTATCAA 8720 0.0 38.228893 1 TATCAAC 11170 0.0 29.71565 2 ATCAACG 11085 0.0 29.715273 3 TCAACGC 11350 0.0 29.021479 4 CAACGCA 11465 0.0 28.688921 5 AACGCAG 11920 0.0 27.65212 6 TAAGGTG 1475 0.0 24.168596 5 TACATGG 11495 0.0 23.78712 2 GTACATG 12050 0.0 23.520655 1 ACGCAGA 14005 0.0 23.399752 7 CGCAGAG 14145 0.0 23.033842 8 GTAAGGT 1395 0.0 22.828785 4 ACATGGG 11865 0.0 21.792816 3 GCAGAGT 15715 0.0 20.27932 9 GAGTACT 7955 0.0 20.269585 12-13 GTACTTT 8230 0.0 19.274889 14-15 TGGTATC 2775 0.0 19.192787 2 AGAGTAC 13870 0.0 19.141705 10-11 AGTACTT 8580 0.0 18.737717 12-13 >>END_MODULE