FastQCFastQC Report
Thu 26 May 2016
SRR938453_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938453_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044592
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70730.23231355794142533No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54580.1792686836200056No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38620.1268478666435437No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA58050.045.995881
GTATCAA97850.040.0051081
ATCAACG122600.031.4390263
TCAACGC124650.030.9601064
TATCAAC125400.030.7749372
CAACGCA125950.030.4518785
AACGCAG131100.029.2513076
ACGCAGA147800.025.6826427
CGCAGAG149450.025.240158
GTACATG118400.024.0009081
TACATGG117450.023.672362
GCAGAGT161350.022.9358279
ACATGGG120100.022.0815663
GAGTACT80300.021.53151912-13
AGAGTAC141950.020.49656110-11
AGTACTT85600.019.83769412-13
GTACTTT87150.019.67773414-15
CAGAGTA155450.019.61799410-11
TGGTATC30250.019.4820652
TAAGGTG13600.019.2204025
Produced by FastQC (version 0.11.5)