FastQCFastQC Report
Thu 26 May 2016
SRR938453_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938453_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3044592
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69690.2288976651058664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59320.19483727212053373No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40820.13407379379568757No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56650.046.011891
GTATCAA94250.038.8596421
ATCAACG118750.030.5077953
TATCAAC120500.030.1575282
TCAACGC121500.029.7781624
CAACGCA123500.029.2959235
AACGCAG129100.028.143776
ACGCAGA147150.024.5624377
CGCAGAG148450.024.1233838
TACATGG116500.023.1905942
TAAGGTG14650.023.0414715
GTACATG120500.022.8549861
ACATGGG121100.021.4357153
GCAGAGT167300.021.0362979
GTAAGGT13400.020.933254
GAGTACT86200.019.9181112-13
AGAGTAC146300.019.86810-11
GTACTTT87900.019.34377514-15
AGTACTT89250.019.2640412-13
TGGTATC30250.019.1833272
Produced by FastQC (version 0.11.5)