##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938453_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3044592 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78147876628461 31.0 31.0 34.0 30.0 34.0 2 32.016051411814786 31.0 31.0 34.0 30.0 34.0 3 32.08830214360413 33.0 31.0 34.0 30.0 34.0 4 35.76128328524807 37.0 35.0 37.0 35.0 37.0 5 35.631164372763244 37.0 35.0 37.0 33.0 37.0 6 35.663902092628504 37.0 35.0 37.0 33.0 37.0 7 35.59270864536201 37.0 35.0 37.0 33.0 37.0 8 35.57703922233258 37.0 35.0 37.0 33.0 37.0 9 37.14451657233547 39.0 37.0 39.0 33.0 39.0 10-11 37.076256687267126 39.0 37.0 39.0 33.0 39.0 12-13 36.96931559302527 39.0 37.0 39.0 33.0 39.0 14-15 38.0254799329434 40.0 37.0 41.0 33.0 41.0 16-17 37.94640283492829 40.0 37.0 41.0 33.0 41.0 18-19 37.934545745374095 40.0 37.0 41.0 33.0 41.0 20-21 37.92138766048127 40.0 37.0 41.0 32.5 41.0 22-23 37.95436909116229 40.0 37.0 41.0 33.0 41.0 24-25 37.88831180007042 40.0 37.0 41.0 32.0 41.0 26-27 37.72372110942944 40.0 37.0 41.0 32.0 41.0 28-29 37.59512653912248 39.5 37.0 41.0 32.0 41.0 30-31 37.366315749368056 39.0 36.0 41.0 31.5 41.0 32-33 37.167540839626454 39.0 36.0 41.0 31.0 41.0 34-35 37.015836276256394 39.0 36.0 41.0 31.0 41.0 36-37 37.04071662147177 39.0 36.0 41.0 31.0 41.0 38-39 36.90288485288012 39.0 36.0 41.0 30.0 41.0 40-41 36.73963802046383 39.0 35.0 41.0 30.0 41.0 42-43 36.528392966939414 39.0 35.0 40.0 30.0 41.0 44-45 36.26887806313621 39.0 35.0 40.0 30.0 41.0 46-47 35.941203944567945 38.0 35.0 40.0 29.0 41.0 48-49 35.57884209115704 38.0 34.5 40.0 28.0 41.0 50-51 35.22335800659005 38.0 34.0 40.0 27.0 41.0 52-53 34.70945663655426 38.0 33.0 40.0 26.0 41.0 54-55 34.398266500076204 38.0 33.0 40.0 25.0 41.0 56-57 34.04169113628362 37.0 33.0 40.0 24.0 41.0 58-59 33.59542001030023 37.0 32.0 39.5 23.0 41.0 60-61 33.183680276372 36.0 32.0 39.0 22.0 40.5 62-63 32.704665846852386 36.0 31.0 39.0 21.5 40.0 64-65 32.20662834297666 35.0 31.0 38.5 19.5 40.0 66-67 31.72300278658027 35.0 30.0 38.0 18.5 40.0 68-69 31.158903064844154 34.5 29.5 37.5 15.5 40.0 70-71 30.594372579314403 34.0 29.0 37.0 12.0 39.0 72-73 30.01291503097952 34.0 28.5 36.0 8.0 39.0 74-75 29.39294723233852 34.0 28.0 36.0 7.0 38.5 76-77 28.11799035798557 31.5 26.5 34.5 4.5 36.5 78-79 28.746647662478257 33.0 28.0 35.0 2.0 37.0 80-81 28.453586391871227 33.0 27.0 35.0 2.0 37.0 82-83 27.965059850383895 33.0 26.0 35.0 2.0 36.0 84-85 27.38190240268647 33.0 26.0 35.0 2.0 36.0 86-87 26.746522358332413 32.0 25.0 35.0 2.0 35.0 88-89 26.01252400978522 32.0 23.5 34.0 2.0 35.0 90-91 25.480042317657013 31.5 21.5 34.0 2.0 35.0 92-93 24.704845345451872 31.0 18.5 34.0 2.0 35.0 94-95 23.923577280633992 31.0 11.0 34.0 2.0 35.0 96-97 22.947461761707316 30.5 2.0 34.0 2.0 35.0 98-99 21.77610694634946 30.0 2.0 34.0 2.0 35.0 100-101 18.624288246175514 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.29739378979331477 1101 2 0.3023709539961388 1101 3 0.3142838535773578 1101 4 0.23318007146443875 1101 5 0.17164880188618525 1101 6 0.20810091156694455 1101 7 0.2778447574137104 1101 8 0.2524786090126767 1101 9 0.3618270308825231 1101 10-11 0.3033529919929734 1101 12-13 0.2660997903893261 1101 14-15 0.4307582343568157 1101 16-17 0.4656531745653396 1101 18-19 0.6013252671240323 1101 20-21 0.6086184182799457 1101 22-23 0.5351485251080277 1101 24-25 0.5524447237715222 1101 26-27 0.49069548482321323 1101 28-29 0.5006320654218399 1101 30-31 0.5986506098992592 1101 32-33 0.5354226494988623 1101 34-35 0.4930731071422585 1101 36-37 0.710235207361066 1101 38-39 0.7664918215558032 1101 40-41 0.8075399601731803 1101 42-43 0.6659166934199376 1101 44-45 0.6682485150443611 1101 46-47 0.7997364167061534 1101 48-49 0.9238004811065963 1101 50-51 1.045283448153036 1101 52-53 1.0241302287292768 1101 54-55 1.1487009716952343 1101 56-57 1.1698749212603872 1101 58-59 1.2250292862190477 1101 60-61 1.2036365363689612 1101 62-63 1.3757891420202384 1101 64-65 1.4943572850044688 1101 66-67 1.6590356453132102 1101 68-69 1.5693828586870673 1101 70-71 1.655394565748228 1101 72-73 1.5083600886772999 1101 74-75 1.593617923503924 1101 76-77 1.4378325576288518 1101 78-79 1.7525570007428364 1101 80-81 1.885603651562878 1101 82-83 1.9497297600447645 1101 84-85 2.152441951736723 1101 86-87 2.2977715513780765 1101 88-89 2.375207645562501 1101 90-91 2.66216954327167 1101 92-93 2.99833920062121 1101 94-95 3.1208805231431676 1101 96-97 3.4911507259595425 1101 98-99 3.818017405542701 1101 100-101 4.4694207968722175 1102 1 0.3691595154222931 1102 2 0.3474793095604767 1102 3 0.3265529480394207 1102 4 0.23147793998264632 1102 5 0.16049175667865256 1102 6 0.18844356345885416 1102 7 0.28237853506773547 1102 8 0.3882957019856832 1102 9 0.3936614056548464 1102 10-11 0.3736107643614588 1102 12-13 0.34963023364213086 1102 14-15 0.5723282547863846 1102 16-17 0.5235247716746656 1102 18-19 0.4937024401985184 1102 20-21 0.6397037753582921 1102 22-23 0.6080949765805599 1102 24-25 0.5640831912443929 1102 26-27 0.4576452038293013 1102 28-29 0.4871866738517525 1102 30-31 0.4680306068852289 1102 32-33 0.34284763046153444 1102 34-35 0.5116881429863227 1102 36-37 0.6054134620949441 1102 38-39 0.6469961784335396 1102 40-41 0.7600074644821504 1102 42-43 0.7126557593085394 1102 44-45 0.6211161025886156 1102 46-47 0.6409132901735575 1102 48-49 0.7172012784286963 1102 50-51 0.7488843344989604 1102 52-53 0.9281984527140068 1102 54-55 0.7817363197414409 1102 56-57 0.945552792253693 1102 58-59 0.9546405106059908 1102 60-61 1.1464028643059692 1102 62-63 1.216639661342505 1102 64-65 1.091650678877997 1102 66-67 1.1405037574193528 1102 68-69 1.2243875419189472 1102 70-71 1.3155715130694858 1102 72-73 1.4802966886459608 1102 74-75 1.592566379509492 1102 76-77 1.5217380711176602 1102 78-79 1.8256366261641297 1102 80-81 2.0574135772200073 1102 82-83 2.260243440970214 1102 84-85 2.4220644937261255 1102 86-87 2.396671016835935 1102 88-89 2.5489977150150516 1102 90-91 2.740096949933889 1102 92-93 3.1197516129571987 1102 94-95 3.0758848820169575 1102 96-97 3.4928066987893125 1102 98-99 3.7310768983065223 1102 100-101 4.258323994943236 1103 1 0.25991781866423125 1103 2 0.2557494304015222 1103 3 0.3150738287471171 1103 4 0.22845467942992315 1103 5 0.21537803854005233 1103 6 0.2107895473936594 1103 7 0.25034205431889944 1103 8 0.26255125192870565 1103 9 0.33742406438913264 1103 10-11 0.34706574785572997 1103 12-13 0.43072120517395973 1103 14-15 0.5662555180283562 1103 16-17 0.5224530877134086 1103 18-19 0.5252219363622785 1103 20-21 0.44888671330596 1103 22-23 0.4567642283488951 1103 24-25 0.39928683264620446 1103 26-27 0.4079612394792562 1103 28-29 0.3613013742431903 1103 30-31 0.50170532759617 1103 32-33 0.5209968721201648 1103 34-35 0.5809689034499783 1103 36-37 0.6589524381568381 1103 38-39 0.7532644243787843 1103 40-41 0.6324683146819794 1103 42-43 0.6836897868681007 1103 44-45 0.7432622755859413 1103 46-47 0.8027113526700091 1103 48-49 0.8292354784157467 1103 50-51 0.8333133331575553 1103 52-53 0.9086513471782567 1103 54-55 0.9695856507596972 1103 56-57 0.9946895644371736 1103 58-59 1.2403941623570276 1103 60-61 1.4163422215434522 1103 62-63 1.2577693390937412 1103 64-65 1.1899389993400433 1103 66-67 1.335194777950619 1103 68-69 1.3816245575147583 1103 70-71 1.4883116314665195 1103 72-73 1.409629060579821 1103 74-75 1.607917491106786 1103 76-77 1.5288455515992112 1103 78-79 1.6827757382137598 1103 80-81 1.9647528040703612 1103 82-83 2.164042720587915 1103 84-85 2.2835380064436386 1103 86-87 2.2872664255457025 1103 88-89 2.500831219882169 1103 90-91 2.595113309096906 1103 92-93 2.808389260564727 1103 94-95 2.7478897705211907 1103 96-97 3.1912564480902184 1103 98-99 3.495769967233546 1103 100-101 3.9563588815420694 1104 1 0.2861567874714943 1104 2 0.2335733673568683 1104 3 0.24351081055927892 1104 4 0.21564435019759287 1104 5 0.12883918181648824 1104 6 0.16243895370302397 1104 7 0.14839598769371065 1104 8 0.2354617221622064 1104 9 0.17897335815941062 1104 10-11 0.25404590352057 1104 12-13 0.3051266749036756 1104 14-15 0.4657329541409396 1104 16-17 0.4816025950631726 1104 18-19 0.5395786676170005 1104 20-21 0.5690932000429143 1104 22-23 0.4419070197849919 1104 24-25 0.4053236409534904 1104 26-27 0.33283197891854144 1104 28-29 0.38459480183536243 1104 30-31 0.5103689017623907 1104 32-33 0.49609767059550336 1104 34-35 0.49151040600900586 1104 36-37 0.5669343453784137 1104 38-39 0.7038332391549318 1104 40-41 0.7787916109557784 1104 42-43 0.8576036025351144 1104 44-45 0.8997379977117461 1104 46-47 1.0028894114760973 1104 48-49 0.9901743970728205 1104 50-51 1.0053947597683432 1104 52-53 1.2744914938604595 1104 54-55 1.2610861320535633 1104 56-57 1.3095550270058283 1104 58-59 1.4676672725741682 1104 60-61 1.2895641808392497 1104 62-63 1.465255778333166 1104 64-65 1.5484007488588887 1104 66-67 1.6357614580703626 1104 68-69 1.6795386282020566 1104 70-71 1.6536375251902378 1104 72-73 1.6723276761270611 1104 74-75 1.7980428552080134 1104 76-77 1.7966444589966812 1104 78-79 2.1007419742111253 1104 80-81 2.3052469514117497 1104 82-83 2.3419400327993465 1104 84-85 2.6354887123887387 1104 86-87 2.7536669906888704 1104 88-89 2.7026266082273978 1104 90-91 3.0274674707945692 1104 92-93 3.0320066436125934 1104 94-95 3.236797583534397 1104 96-97 3.549463518869164 1104 98-99 4.064140450229445 1104 100-101 4.53793298286595 1105 1 0.2410103847726326 1105 2 0.2354094137864422 1105 3 0.18464334622202472 1105 4 0.14817699649929494 1105 5 0.11152337762911912 1105 6 0.1436641589156551 1105 7 0.16798942455221066 1105 8 0.20929749495697791 1105 9 0.21626256375125763 1105 10-11 0.32055117233713304 1105 12-13 0.3638264032618821 1105 14-15 0.4073530768564524 1105 16-17 0.4486568033024909 1105 18-19 0.36784433469070876 1105 20-21 0.30197122948451494 1105 22-23 0.24176363083951458 1105 24-25 0.26985960103727535 1105 26-27 0.39847545970404497 1105 28-29 0.36666051835725 1105 30-31 0.3896383183869929 1105 32-33 0.4654675402796187 1105 34-35 0.4984629923157371 1105 36-37 0.5442085980133555 1105 38-39 0.5172903617126252 1105 40-41 0.41302106235570335 1105 42-43 0.43288807769225457 1105 44-45 0.48019578658094275 1105 46-47 0.6633688433499074 1105 48-49 0.819990086796814 1105 50-51 0.7817076277681068 1105 52-53 0.8853311487143216 1105 54-55 0.8255781382006475 1105 56-57 0.9170748130104798 1105 58-59 1.0491397725458924 1105 60-61 1.0498265362854795 1105 62-63 1.1742647732693072 1105 64-65 1.4160816238869671 1105 66-67 1.3809340930819403 1105 68-69 1.3569835255324456 1105 70-71 1.38323980131468 1105 72-73 1.5017101287028645 1105 74-75 1.6691466969221302 1105 76-77 1.5135202390309246 1105 78-79 1.7334674037719786 1105 80-81 1.9917790558303565 1105 82-83 2.1499112084971266 1105 84-85 2.365056891893172 1105 86-87 2.3226855520700624 1105 88-89 2.520967950406103 1105 90-91 2.6639856510800435 1105 92-93 2.8007384271028286 1105 94-95 2.8430420589931806 1105 96-97 3.28011002418107 1105 98-99 3.644461421697631 1105 100-101 4.140894918002655 1106 1 0.2283137786629048 1106 2 0.2568941887350604 1106 3 0.21914983737809735 1106 4 0.12929777559097033 1106 5 0.09897960831475672 1106 6 0.1510114771875024 1106 7 0.13790330489469937 1106 8 0.06707919260735196 1106 9 0.18316038677750868 1106 10-11 0.2932956666013453 1106 12-13 0.13727230052327144 1106 14-15 0.12627373044989554 1106 16-17 0.19663981430844046 1106 18-19 0.31452298614124885 1106 20-21 0.37057891283131283 1106 22-23 0.34708119362474577 1106 24-25 0.2539879406115304 1106 26-27 0.22417847709960625 1106 28-29 0.37157237857753955 1106 30-31 0.43840414267757666 1106 32-33 0.4125260532997501 1106 34-35 0.3700275100482031 1106 36-37 0.49132234045224266 1106 38-39 0.4826267853443582 1106 40-41 0.4042946548764519 1106 42-43 0.6052672187303472 1106 44-45 0.17617987498540089 1106 46-47 0.10464818185442226 1106 48-49 0.5813287395728537 1106 50-51 0.5149201679899917 1106 52-53 0.7953931734757944 1106 54-55 0.8526831968755317 1106 56-57 0.9004187886279666 1106 58-59 1.2526931590014314 1106 60-61 1.0157736698361575 1106 62-63 1.113020658388713 1106 64-65 0.7665353866672646 1106 66-67 0.9858467817435113 1106 68-69 0.9472472481312231 1106 70-71 0.9328755951611036 1106 72-73 1.3838691358988306 1106 74-75 1.714700187039039 1106 76-77 1.6681310670558638 1106 78-79 1.9327239945534238 1106 80-81 1.8307769626353867 1106 82-83 2.1359282348772446 1106 84-85 2.21324794787116 1106 86-87 2.518120373283935 1106 88-89 2.8146451272311594 1106 90-91 2.7646948499220976 1106 92-93 2.2222283213937217 1106 94-95 2.729629084837999 1106 96-97 3.0842231714167525 1106 98-99 3.310101086701465 1106 100-101 3.818962795383147 1107 1 0.19895626877417172 1107 2 0.14648637879580662 1107 3 0.12176588361175078 1107 4 0.15167063919428614 1107 5 0.036236086818320246 1107 6 0.04715795359626185 1107 7 0.13014419555801737 1107 8 0.18329670767148798 1107 9 0.1721152005243809 1107 10-11 0.22103154820769078 1107 12-13 0.2669250860025514 1107 14-15 0.33576151894816064 1107 16-17 0.31492395044634947 1107 18-19 0.35289710662970464 1107 20-21 0.25553572280752945 1107 22-23 0.26304810181051863 1107 24-25 0.24932021421818007 1107 26-27 0.24963209557335375 1107 28-29 0.23290760339978078 1107 30-31 0.1372114880622206 1107 32-33 0.30206633523633286 1107 34-35 0.3801032312168573 1107 36-37 0.3441048931613153 1107 38-39 0.3152130729738971 1107 40-41 0.5057146578338276 1107 42-43 0.6070082202952918 1107 44-45 0.6081196985285899 1107 46-47 0.4941405604479385 1107 48-49 0.5824246633218237 1107 50-51 0.5457583686726508 1107 52-53 0.663890487191857 1107 54-55 0.7761692355925263 1107 56-57 0.738444801221469 1107 58-59 0.7037169966907371 1107 60-61 0.8204404720492562 1107 62-63 0.9746024853082389 1107 64-65 0.9643131952510515 1107 66-67 1.0976635044771115 1107 68-69 1.2289356946458305 1107 70-71 1.2027502455129095 1107 72-73 1.3572924843887222 1107 74-75 1.3594876563870564 1107 76-77 1.2606639986788473 1107 78-79 1.46912591438387 1107 80-81 1.7257982495871929 1107 82-83 1.9550124030534057 1107 84-85 2.1528590836270745 1107 86-87 2.268346489385621 1107 88-89 2.484099583628332 1107 90-91 2.6857388963529125 1107 92-93 2.9282484315560104 1107 94-95 2.923841868099249 1107 96-97 3.2038108638002782 1107 98-99 3.539925461647556 1107 100-101 4.010324328536573 1108 1 0.1512464218590388 1108 2 0.13205303759403364 1108 3 0.1538385180527868 1108 4 0.10394855248407708 1108 5 0.03275124230743387 1108 6 0.025933274672318873 1108 7 0.11102913274164194 1108 8 0.07444733583525931 1108 9 0.10949728321293861 1108 10-11 0.13067475818137808 1108 12-13 0.22608744688416493 1108 14-15 0.19575574875905488 1108 16-17 0.2596298019426655 1108 18-19 0.2506895721615692 1108 20-21 0.23937219914538588 1108 22-23 0.2572512474378499 1108 24-25 0.2905036275473165 1108 26-27 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2316 64-65 0.25254807934684464 2316 66-67 0.3957776801314452 2316 68-69 0.40656874040292834 2316 70-71 0.42963448458310083 2316 72-73 0.5041418038335905 2316 74-75 0.7586922111376317 2316 76-77 0.6704306993744815 2316 78-79 0.7939631392659656 2316 80-81 1.0272988156878569 2316 82-83 1.2528647057204942 2316 84-85 1.2617794650419363 2316 86-87 1.4993153661925298 2316 88-89 1.7030804862624116 2316 90-91 1.9094385953199868 2316 92-93 1.8882055347276712 2316 94-95 2.602163431669762 2316 96-97 2.639091641500123 2316 98-99 2.938609900825494 2316 100-101 3.357248918313264 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 19.0 4 24.0 5 78.0 6 198.0 7 489.0 8 1141.0 9 2092.0 10 3428.0 11 5328.0 12 7803.0 13 10943.0 14 14342.0 15 18883.0 16 23579.0 17 29347.0 18 28772.0 19 26364.0 20 27094.0 21 29262.0 22 32519.0 23 36847.0 24 42695.0 25 49984.0 26 58956.0 27 70999.0 28 84619.0 29 99981.0 30 118401.0 31 141031.0 32 166829.0 33 203829.0 34 252355.0 35 318854.0 36 405402.0 37 459338.0 38 257098.0 39 15660.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.542054896025476 19.077498725609214 14.85253196487411 23.5279144134912 2 16.049267098836506 20.37484293097375 39.289469671876894 24.286420298312855 3 19.646165705781634 23.813197774806387 29.535874957178127 27.004761562233853 4 13.542011540462564 16.677636937888558 35.97059967312533 33.80975184852355 5 14.039566700213744 37.2936747923802 33.92405121556917 14.742707291836881 6 33.24626091115 35.70672195157841 16.8271479396911 14.219869197580499 7 29.47770998544304 31.99282531124039 19.968028556864105 18.561436146452465 8 26.67493706874353 35.415024410495725 19.142564915101925 18.76747360565882 9 26.407282158003436 16.680822914860187 19.558055726350197 37.35383920078618 10-11 25.400973266697147 26.465335913646232 26.95729017221355 21.17640064744307 12-13 26.11565030716759 23.46787352788157 27.432542685522392 22.983933479428444 14-15 22.73830122394068 25.182815956949238 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22.862253748851504 40-41 23.023563625302998 26.332896954694636 28.041737309256792 22.601802110745574 42-43 22.72320640387333 26.338868893208282 27.89004100140996 23.04788370150842 44-45 22.698068127920834 26.576428769443204 28.009336812384795 22.716166290251167 46-47 22.948915461701663 26.81163525932305 27.487243358343267 22.75220592063202 48-49 22.768255976498658 26.764390762374727 27.506789087010674 22.96056417411594 50-51 22.9833422672069 26.913080636091795 27.396872881489536 22.706704215211758 52-53 23.20013650433293 26.714613977833483 27.313216352141765 22.772033165691823 54-55 22.82599770346897 26.760843489045495 27.477179208248593 22.93597959923694 56-57 22.80569941719613 26.955877831906538 27.301671291260043 22.936751459637282 58-59 22.808901816729467 26.657906872250862 27.334138695759563 23.199052615260108 60-61 22.629255414190144 26.840936322502323 27.543411399622673 22.986396863684856 62-63 22.632375700914935 26.846634951415492 27.596784725178285 22.92420462249129 64-65 22.448360896960907 26.76325760561678 27.512372757991876 23.27600873943044 66-67 22.40259542975104 26.84420606109376 27.383606783524506 23.36959172563069 68-69 22.808653466065227 26.544862376085266 27.442456414810106 23.2040277430394 70-71 22.668768097663 26.575170006358817 27.236769327384426 23.519292568593755 72-73 22.690022618797954 26.669686228842192 27.176897130534588 23.46339402182526 74-75 22.500584643196856 26.9699355447298 26.988328813844355 23.54115099822899 76-77 22.52526775344611 26.56848602374308 27.306630904896288 23.59961531791452 78-79 22.67906504385481 26.68424866123277 27.247788866291444 23.38889742862098 80-81 22.658261379235935 26.781688118094003 27.134280575899922 23.425769926770144 82-83 22.63930568495325 26.754012779209567 27.178491959989547 23.428189575847632 84-85 22.586448971494104 26.74502917695386 27.084582536594166 23.58393931495787 86-87 22.561295855494613 26.71838115286495 26.98203372138358 23.738289270256857 88-89 22.49710880516221 26.77126534977299 26.863034715030505 23.8685911300343 90-91 22.4100164358941 26.711431814688254 27.051087252131346 23.827464497286304 92-93 22.409584379318126 26.52171285422385 27.081888826008456 23.986813940449565 94-95 22.262588878903973 26.361955887685447 27.189242433797368 24.186212799613216 96-97 22.2785224224662 26.193776734908443 27.181807477292107 24.34589336533325 98-99 22.090201248640213 26.353990945256378 26.90992750424359 24.645880301859822 100-101 22.268744026250012 26.05937784317654 27.1315817997287 24.54029633084475 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 382.0 1 265.5 2 203.0 3 285.5 4 261.0 5 260.5 6 468.0 7 1043.5 8 1679.0 9 1935.0 10 1823.5 11 1641.0 12 1662.5 13 1797.0 14 1998.0 15 2182.0 16 2302.5 17 2534.5 18 2758.5 19 2981.5 20 3463.5 21 4124.0 22 4874.5 23 5886.5 24 7284.5 25 9260.5 26 12297.0 27 16384.5 28 20230.5 29 24283.0 30 29138.0 31 35764.5 32 44661.5 33 49520.0 34 52651.0 35 58288.0 36 64620.5 37 70824.5 38 75918.0 39 80082.5 40 84957.0 41 91864.5 42 96960.0 43 101800.5 44 107430.0 45 113318.5 46 116962.5 47 120970.0 48 124770.0 49 124085.5 50 121973.0 51 117753.5 52 113569.0 53 112174.5 54 112606.5 55 107044.0 56 96513.5 57 87242.5 58 78923.0 59 68259.0 60 55799.0 61 46258.0 62 38161.0 63 28588.5 64 20271.5 65 15073.5 66 11124.0 67 8400.0 68 6336.5 69 4773.5 70 3581.5 71 2474.0 72 1831.5 73 1472.5 74 1124.0 75 844.0 76 580.0 77 362.0 78 228.5 79 134.5 80 76.0 81 42.5 82 22.5 83 13.0 84 7.5 85 2.0 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.04414384587491526 3 9.853537025650728E-5 4 0.0 5 0.10165565698129668 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0011495793196592515 26-27 0.012333343843772828 28-29 0.0 30-31 0.0 32-33 4.10564042735447E-4 34-35 0.01609411047522952 36-37 0.0036786538229096047 38-39 0.012908133503602452 40-41 0.007143814343596778 42-43 0.006059925270775197 44-45 0.0027589903671822037 46-47 4.926768512825364E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 3.120286724789397E-4 68-69 2.79183549060104E-4 70-71 0.0 72-73 1.642256170941788E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.642256170941788E-5 82-83 0.0012316921282063409 84-85 0.0049760361979536176 86-87 0.001806481788035967 88-89 9.853537025650728E-5 90-91 0.0011824244430780873 92-93 4.2698660444486484E-4 94-95 0.0 96-97 1.642256170941788E-5 98-99 0.0 100-101 7.225927152143867E-4 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3044592.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.491132507848434 #Duplication Level Percentage of deduplicated Percentage of total 1 72.87150262758722 37.52236197843119 2 14.841399564437369 15.284009431487371 3 5.11099264731957 7.895123989493132 4 2.3380261098209303 4.815504489103958 5 1.3147042783657392 3.384780610298276 6 0.7979660685910481 2.465290594475312 7 0.5256656199914529 1.8946982665660144 8 0.36949852716099735 1.5220718098801398 9 0.2683334616161892 1.2435114445531974 >10 1.4119639370885406 13.099902304782502 >50 0.09763993664206587 3.4422213507702457 >100 0.04726092679758159 4.364286975289901 >500 0.0031938573300586467 1.138524256412373 >1k 0.0017246829578917754 1.5033848535112728 >5k 1.2775429317716855E-4 0.42432764494506275 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6969 0.2288976651058664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5932 0.19483727212053373 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4082 0.13407379379568757 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 3.284512341883576E-5 7 0.0 0.0 0.0 0.0 3.284512341883576E-5 8 0.0 0.0 0.0 0.0 3.284512341883576E-5 9 0.0 0.0 0.0 0.0 3.284512341883576E-5 10-11 0.0 0.0 0.0 0.0 3.284512341883576E-5 12-13 0.0 0.0 0.0 1.642256170941788E-5 4.926768512825364E-5 14-15 0.0 0.0 0.0 3.284512341883576E-5 6.569024683767152E-5 16-17 0.0 0.0 0.0 6.569024683767152E-5 6.569024683767152E-5 18-19 0.0 0.0 0.0 9.853537025650728E-5 9.853537025650728E-5 20-21 0.0 0.0 0.0 1.8064817880359668E-4 9.853537025650728E-5 22-23 0.0 0.0 0.0 3.777189193166112E-4 9.853537025650728E-5 24-25 0.0 0.0 0.0 6.076347832484614E-4 9.853537025650728E-5 26-27 0.0 0.0 0.0 0.0010838890728215801 1.1495793196592516E-4 28-29 0.0 0.0 0.0 0.003514428205815426 1.3138049367534304E-4 30-31 0.0 0.0 0.0 0.013285852422919065 1.3138049367534304E-4 32-33 0.0 0.0 0.0 0.03366625150430665 1.3138049367534304E-4 34-35 0.0 0.0 0.0 0.06473773825852529 1.6422561709417878E-4 36-37 3.284512341883576E-5 0.0 0.0 0.1166494558219952 1.6422561709417878E-4 38-39 3.284512341883576E-5 0.0 0.0 0.1957405130145517 1.6422561709417878E-4 40-41 4.926768512825364E-5 0.0 0.0 0.2823531034700216 1.6422561709417878E-4 42-43 6.569024683767152E-5 0.0 0.0 0.37083786596036517 1.6422561709417878E-4 44-45 6.569024683767152E-5 0.0 0.0 0.4777487426886755 1.6422561709417878E-4 46-47 6.569024683767152E-5 0.0 0.0 0.6068629228481188 1.6422561709417878E-4 48-49 6.569024683767152E-5 0.0 0.0 0.7446482155901348 1.6422561709417878E-4 50-51 6.569024683767152E-5 0.0 0.0 0.8924841160983147 1.6422561709417878E-4 52-53 6.569024683767152E-5 0.0 0.0 1.0369041237709355 2.2991586393185032E-4 54-55 6.569024683767152E-5 0.0 0.0 1.1951683509645956 2.2991586393185032E-4 56-57 6.569024683767152E-5 0.0 0.0 1.3832231050991397 2.2991586393185032E-4 58-59 6.569024683767152E-5 0.0 0.0 1.5855162202357493 2.2991586393185032E-4 60-61 6.569024683767152E-5 0.0 0.0 1.8052336733460508 2.2991586393185032E-4 62-63 6.569024683767152E-5 0.0 0.0 2.0279236101257574 2.627609873506861E-4 64-65 6.569024683767152E-5 0.0 0.0 2.256755584984786 2.627609873506861E-4 66-67 6.569024683767152E-5 0.0 0.0 2.5263647805682994 2.627609873506861E-4 68-69 6.569024683767152E-5 0.0 0.0 2.821576749856795 2.627609873506861E-4 70-71 6.569024683767152E-5 0.0 0.0 3.1352805236300956 2.627609873506861E-4 72-73 6.569024683767152E-5 0.0 0.0 3.451184920672458 3.120286724789397E-4 74-75 6.569024683767152E-5 0.0 0.0 3.784447965441675 3.777189193166112E-4 76-77 6.569024683767152E-5 0.0 0.0 4.1429689101199765 3.941414810260291E-4 78-79 6.569024683767152E-5 0.0 0.0 4.553303036991492 3.941414810260291E-4 80-81 9.853537025650728E-5 0.0 0.0 4.978286088907808 4.269866044448649E-4 82-83 9.853537025650728E-5 0.0 0.0 5.4167520639875555 4.5983172786370064E-4 84-85 9.853537025650728E-5 0.0 0.0 5.861409344831754 4.926768512825364E-4 86-87 9.853537025650728E-5 0.0 0.0 6.326184263769989 5.090994129919543E-4 88-89 9.853537025650728E-5 0.0 0.0 6.825167378748942 5.255219747013722E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5665 0.0 46.01189 1 GTATCAA 9425 0.0 38.859642 1 ATCAACG 11875 0.0 30.507795 3 TATCAAC 12050 0.0 30.157528 2 TCAACGC 12150 0.0 29.778162 4 CAACGCA 12350 0.0 29.295923 5 AACGCAG 12910 0.0 28.14377 6 ACGCAGA 14715 0.0 24.562437 7 CGCAGAG 14845 0.0 24.123383 8 TACATGG 11650 0.0 23.190594 2 TAAGGTG 1465 0.0 23.041471 5 GTACATG 12050 0.0 22.854986 1 ACATGGG 12110 0.0 21.435715 3 GCAGAGT 16730 0.0 21.036297 9 GTAAGGT 1340 0.0 20.93325 4 GAGTACT 8620 0.0 19.91811 12-13 AGAGTAC 14630 0.0 19.868 10-11 GTACTTT 8790 0.0 19.343775 14-15 AGTACTT 8925 0.0 19.26404 12-13 TGGTATC 3025 0.0 19.183327 2 >>END_MODULE