FastQCFastQC Report
Thu 26 May 2016
SRR938452_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938452_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052481
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT67280.2204108723363061No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52850.17313785081708943No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35090.11495567048574586No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.047.6464651
GTATCAA87950.043.0037651
ATCAACG110850.033.4833953
TATCAAC112550.032.9776462
TCAACGC114100.032.612964
CAACGCA113800.032.5736545
AACGCAG119200.031.2891396
ACGCAGA137300.026.917717
CGCAGAG137350.026.7695678
GTACATG117900.024.7170451
TACATGG115700.024.398732
GCAGAGT153700.023.2412139
ACATGGG120150.022.78313
GAGTACT76400.021.4813912-13
TGGTATC25050.021.2483122
AGAGTAC135700.020.4948210-11
GTGGTAT28600.019.8449541
GTACTTT81500.019.67185214-15
CAGAGTA146800.019.52757310-11
AGTACTT83300.019.18879712-13
Produced by FastQC (version 0.11.5)