##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938452_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3052481 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.92020032229521 31.0 31.0 34.0 27.0 34.0 2 31.099264172324087 31.0 31.0 34.0 27.0 34.0 3 31.155824720940114 31.0 31.0 34.0 27.0 34.0 4 34.81873400686196 37.0 35.0 37.0 32.0 37.0 5 34.80368821296513 37.0 35.0 37.0 32.0 37.0 6 34.765623766372336 37.0 35.0 37.0 32.0 37.0 7 34.676666619710325 37.0 35.0 37.0 30.0 37.0 8 34.6265853907035 37.0 35.0 37.0 30.0 37.0 9 36.08476514677733 39.0 35.0 39.0 30.0 39.0 10-11 35.915920524976244 38.0 35.0 39.0 30.0 39.0 12-13 35.72884696088198 38.0 35.0 39.0 30.0 39.0 14-15 36.85475437848753 39.0 36.0 41.0 30.5 41.0 16-17 36.78002647682328 39.0 36.0 41.0 30.0 41.0 18-19 36.76638298485723 39.0 36.0 41.0 30.0 41.0 20-21 36.68916628801293 39.0 36.0 41.0 30.0 41.0 22-23 36.53018053183624 39.0 36.0 41.0 30.0 41.0 24-25 36.38496128231429 39.0 36.0 41.0 29.0 41.0 26-27 36.168268205436824 39.0 35.0 40.5 28.5 41.0 28-29 35.928351232980646 39.0 35.0 40.0 27.0 41.0 30-31 35.658927606756606 38.0 34.5 40.0 27.0 41.0 32-33 35.37564017597489 38.0 34.0 40.0 26.5 41.0 34-35 35.07386729024685 38.0 34.0 40.0 25.0 41.0 36-37 34.85982844774463 38.0 34.0 40.0 25.0 41.0 38-39 34.62562567957016 38.0 33.0 40.0 24.0 41.0 40-41 34.35499729564246 38.0 33.0 40.0 24.0 41.0 42-43 33.9511109160057 38.0 33.0 40.0 23.0 41.0 44-45 33.494491202402244 38.0 32.0 40.0 20.0 41.0 46-47 33.023954776458886 37.0 31.5 40.0 17.0 41.0 48-49 32.54928712086988 37.0 31.0 40.0 14.5 41.0 50-51 31.829481657707287 36.0 30.0 38.5 13.0 40.5 52-53 31.84953731079735 36.0 30.5 39.0 9.5 40.0 54-55 31.833369478794463 36.0 30.0 39.0 8.0 40.0 56-57 31.407288857817626 36.0 30.0 39.0 6.5 40.0 58-59 30.944422258484167 35.5 29.0 39.0 2.0 40.0 60-61 30.387810440097745 35.0 28.0 39.0 2.0 40.0 62-63 29.662907811711193 34.5 27.5 38.0 2.0 40.0 64-65 28.989170448562987 34.0 26.0 38.0 2.0 40.0 66-67 28.300442492516744 34.0 25.5 37.5 2.0 39.5 68-69 27.454534360738037 33.0 23.5 36.5 2.0 39.0 70-71 26.61884447438002 32.0 21.5 36.0 2.0 39.0 72-73 25.724420397702723 31.5 19.0 35.5 2.0 38.5 74-75 24.795396924665543 31.0 12.0 35.0 2.0 37.5 76-77 23.85758994077277 30.5 7.0 34.5 2.0 37.0 78-79 22.879215300603015 29.5 2.0 34.0 2.0 36.0 80-81 21.919085000037676 29.0 2.0 34.0 2.0 35.5 82-83 20.93581106647347 27.5 2.0 34.0 2.0 35.0 84-85 19.966003883398454 26.0 2.0 33.0 2.0 35.0 86-87 18.992096429101444 25.5 2.0 32.5 2.0 35.0 88-89 17.918303340790658 23.0 2.0 32.0 2.0 34.0 90-91 16.769996930365824 19.0 2.0 31.5 2.0 34.0 92-93 15.630368379033317 6.0 2.0 31.0 2.0 34.0 94-95 14.382859221728161 2.0 2.0 30.5 2.0 34.0 96-97 12.981237721053791 2.0 2.0 29.5 2.0 34.0 98-99 11.308952782998485 2.0 2.0 28.0 2.0 34.0 100-101 8.038049540685101 2.0 2.0 10.0 2.0 28.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.6878973787832017 1101 2 0.549218425025245 1101 3 0.47083031286066657 1101 4 0.41039271153070445 1101 5 0.4360332512910219 1101 6 0.49047237907952734 1101 7 0.5115856025262815 1101 8 0.4457490293140651 1101 9 0.48736749757487985 1101 10-11 0.6783143528759794 1101 12-13 0.6351050728119887 1101 14-15 0.7183843486631005 1101 16-17 0.8691126732244143 1101 18-19 0.7834326249034618 1101 20-21 0.8863603768630597 1101 22-23 0.8643645153007427 1101 24-25 1.0080447983667682 1101 26-27 0.9762960157993845 1101 28-29 0.9915173559261774 1101 30-31 1.1648901745764917 1101 32-33 1.0327411704378164 1101 34-35 1.0194922083324727 1101 36-37 1.2783186706135936 1101 38-39 1.2878240040547198 1101 40-41 1.218603326438462 1101 42-43 1.2775276282708532 1101 44-45 1.3792210776842637 1101 46-47 1.5383077061591024 1101 48-49 1.2980744834762987 1101 50-51 1.5709366846888777 1101 52-53 1.677497702890708 1101 54-55 1.8556394640580613 1101 56-57 2.025192110560919 1101 58-59 1.8455955098901313 1101 60-61 1.9869671889313913 1101 62-63 1.8202529186210512 1101 64-65 1.8535498269914967 1101 66-67 2.0258771475143327 1101 68-69 2.166796012116187 1101 70-71 2.507555816736165 1101 72-73 2.6428589686626403 1101 74-75 2.665566666626006 1101 76-77 2.873510917928666 1101 78-79 3.044634981847217 1101 80-81 3.4098384596847424 1101 82-83 3.5042339382315717 1101 84-85 3.458640634900533 1101 86-87 3.5769246902658303 1101 88-89 3.8653238188572345 1101 90-91 3.8179575815643823 1101 92-93 3.829974183450057 1101 94-95 3.803885135354447 1101 96-97 3.6814715983682103 1101 98-99 3.303575224811624 1101 100-101 2.326257993370124 1102 1 0.878327721876893 1102 2 0.7726344315812987 1102 3 0.7146556179505481 1102 4 0.5862534900241485 1102 5 0.5752438767336798 1102 6 0.6708875754206431 1102 7 0.6972177419570968 1102 8 0.7728169204848498 1102 9 0.7746024492719172 1102 10-11 0.8805882974661756 1102 12-13 0.8583457589773644 1102 14-15 0.983789283137412 1102 16-17 1.0741059177654293 1102 18-19 0.9482501138093156 1102 20-21 1.0643894308995243 1102 22-23 0.9318816937036658 1102 24-25 1.0021123304608537 1102 26-27 0.9960712755981618 1102 28-29 0.9496727555680238 1102 30-31 0.9663030930973804 1102 32-33 0.94811332097715 1102 34-35 0.9606874997207058 1102 36-37 1.1725012001689592 1102 38-39 1.210681687299676 1102 40-41 1.3177980507862728 1102 42-43 1.6784043035800593 1102 44-45 1.6339534750707898 1102 46-47 1.6243033358643473 1102 48-49 1.7340362655872852 1102 50-51 1.6694470604405787 1102 52-53 1.5999045535410978 1102 54-55 1.5565419606130853 1102 56-57 1.9249595848628438 1102 58-59 1.9783900692362693 1102 60-61 2.1162182957789035 1102 62-63 2.090157865382462 1102 64-65 2.279930973276315 1102 66-67 2.4101460669868935 1102 68-69 2.5239520121753607 1102 70-71 2.3116387034243004 1102 72-73 2.5891027904221957 1102 74-75 2.5570183426901636 1102 76-77 2.788134594666232 1102 78-79 3.3103209302113257 1102 80-81 3.575120963308823 1102 82-83 3.769728672445737 1102 84-85 3.764106278644853 1102 86-87 3.873284126898085 1102 88-89 4.094780065233003 1102 90-91 4.039145283071267 1102 92-93 3.923414388865888 1102 94-95 3.7683941875227784 1102 96-97 3.4073282139193264 1102 98-99 3.0710308210103747 1102 100-101 2.0519304858331218 1103 1 0.5880660796445945 1103 2 0.658533838373117 1103 3 0.552141548853875 1103 4 0.5648582915977087 1103 5 0.5293848472409266 1103 6 0.49814028813889166 1103 7 0.5928095243770457 1103 8 0.4936572817901208 1103 9 0.5714452194660851 1103 10-11 0.6779431494587556 1103 12-13 0.7016376803258026 1103 14-15 0.8204015183148385 1103 16-17 0.7922792448275189 1103 18-19 1.057609916861253 1103 20-21 0.9414408709191378 1103 22-23 0.9376624307998256 1103 24-25 0.9078508734197825 1103 26-27 1.0980500859339344 1103 28-29 1.049368417638938 1103 30-31 1.1011740397156586 1103 32-33 1.209521784426606 1103 34-35 1.0783004128804023 1103 36-37 1.169854333530843 1103 38-39 1.1669716476554228 1103 40-41 1.3677477720699684 1103 42-43 1.2854804322556959 1103 44-45 1.48338862429528 1103 46-47 1.7959324609725869 1103 48-49 1.5742667444854845 1103 50-51 1.6091320670011875 1103 52-53 1.7851962665494092 1103 54-55 1.7806463172717422 1103 56-57 1.8203686312437775 1103 58-59 1.8948574273536636 1103 60-61 2.1533193235551273 1103 62-63 2.257988115176662 1103 64-65 2.367605865870665 1103 66-67 2.521508600907925 1103 68-69 2.585821314169287 1103 70-71 2.6895371828570234 1103 72-73 2.8372818310544865 1103 74-75 2.7936986917615947 1103 76-77 2.8931029455559027 1103 78-79 3.107338544772965 1103 80-81 3.167800403259694 1103 82-83 3.2039696532296915 1103 84-85 3.168769099086173 1103 86-87 3.3594417842571396 1103 88-89 3.6577292986360277 1103 90-91 3.566324375262475 1103 92-93 3.6298650762655544 1103 94-95 3.4554950223914798 1103 96-97 3.453138490418942 1103 98-99 3.2721558577066467 1103 100-101 2.3475156743230663 1104 1 0.7296825106404405 1104 2 0.5573446726784006 1104 3 0.5617493393143427 1104 4 0.5048397699242955 1104 5 0.5398684857013549 1104 6 0.6176297626737792 1104 7 0.6124458836450941 1104 8 0.5340312535969929 1104 9 0.6345109714067121 1104 10-11 0.7088986428385624 1104 12-13 0.6772215607911249 1104 14-15 0.8286363460501676 1104 16-17 0.9053229367561713 1104 18-19 0.9392208219333114 1104 20-21 0.7886038114095228 1104 22-23 0.9127843069055501 1104 24-25 0.9138646292569419 1104 26-27 1.034219441301076 1104 28-29 0.9706455175991877 1104 30-31 1.045725708195178 1104 32-33 1.0983550500509978 1104 34-35 1.0835589409315602 1104 36-37 1.3031798584935999 1104 38-39 1.316181418711814 1104 40-41 1.3065074861197914 1104 42-43 1.4004111288715606 1104 44-45 1.5606910015345932 1104 46-47 1.5950975659651405 1104 48-49 1.7770288873207818 1104 50-51 1.702040592212942 1104 52-53 1.7899565331916172 1104 54-55 2.050493993981174 1104 56-57 2.1900751566390007 1104 58-59 2.2234014447221 1104 60-61 2.218085771369008 1104 62-63 2.305832731878496 1104 64-65 2.381057067376858 1104 66-67 2.421363954938357 1104 68-69 2.71821371154655 1104 70-71 2.7614625413868197 1104 72-73 2.8361258099817164 1104 74-75 2.743287578320711 1104 76-77 2.9038082762158233 1104 78-79 3.2001706790026816 1104 80-81 3.38579061604052 1104 82-83 3.4692388945836967 1104 84-85 3.5434376528667393 1104 86-87 3.605156026288231 1104 88-89 3.550651474845285 1104 90-91 3.3153851320300944 1104 92-93 3.246777187867375 1104 94-95 3.409548167362887 1104 96-97 3.196145084396777 1104 98-99 2.9420189873452554 1104 100-101 2.1894652442620384 1105 1 0.5023503357730554 1105 2 0.4433470505821866 1105 3 0.38894432742482365 1105 4 0.3894199784481671 1105 5 0.41997183305822716 1105 6 0.39862482395052723 1105 7 0.3751173670912209 1105 8 0.5260927132640845 1105 9 0.5718120506092035 1105 10-11 0.5464626633251228 1105 12-13 0.6017299749122174 1105 14-15 0.6866251881939363 1105 16-17 0.6985321922893704 1105 18-19 0.6126020182018266 1105 20-21 0.6923055674000196 1105 22-23 0.587743150878481 1105 24-25 0.7229509654559578 1105 26-27 0.6034713161867487 1105 28-29 0.7135392435915051 1105 30-31 0.8154828876354543 1105 32-33 0.8414819935300528 1105 34-35 0.8660699158721172 1105 36-37 0.9280723955340235 1105 38-39 0.9407698584411008 1105 40-41 1.0026959807237787 1105 42-43 1.2541746189026597 1105 44-45 1.3853274566287936 1105 46-47 1.442710516394989 1105 48-49 1.5471107420857422 1105 50-51 1.2902768278083308 1105 52-53 1.294630028710177 1105 54-55 1.5857555633976794 1105 56-57 1.6248222757171042 1105 58-59 1.7771786534288836 1105 60-61 1.8466434812647385 1105 62-63 1.7230702478836157 1105 64-65 1.8841506287450684 1105 66-67 2.0290990015818515 1105 68-69 2.0972973040104215 1105 70-71 2.281340902462368 1105 72-73 2.367420395677712 1105 74-75 2.532959701971709 1105 76-77 2.66595387709382 1105 78-79 2.9996356506507524 1105 80-81 3.39459114649598 1105 82-83 3.4957113657744756 1105 84-85 3.3936525787858898 1105 86-87 3.522148892576876 1105 88-89 3.3903098798205917 1105 90-91 3.3408950094765935 1105 92-93 3.5161045154693493 1105 94-95 3.5069553376573825 1105 96-97 3.4109223845614043 1105 98-99 3.264583922410189 1105 100-101 2.595092187741095 1106 1 0.41283514263873045 1106 2 0.3315602939960556 1106 3 0.2355294742585059 1106 4 0.2440616394335393 1106 5 0.21309718628717889 1106 6 -0.024809506162007722 1106 7 0.24525738115629991 1106 8 -0.046857876646356544 1106 9 0.259530680467428 1106 10-11 0.2659542244864923 1106 12-13 0.3297613563201054 1106 14-15 0.5353398379086443 1106 16-17 0.10180895800002787 1106 18-19 0.448808021946725 1106 20-21 0.4333428026410857 1106 22-23 0.4120740468717585 1106 24-25 0.4578805181146208 1106 26-27 0.316254317951703 1106 28-29 0.4933001393739218 1106 30-31 0.3962611551505475 1106 32-33 0.5030684509542596 1106 34-35 0.4178567427422806 1106 36-37 0.35366708115147105 1106 38-39 0.5696682450917692 1106 40-41 0.6917019453576856 1106 42-43 0.11548122183962306 1106 44-45 0.596079301481339 1106 46-47 0.8893304641615529 1106 48-49 0.6488367009150053 1106 50-51 0.4969964879478148 1106 52-53 0.6921911003680599 1106 54-55 0.6269181832888044 1106 56-57 1.1519778043626054 1106 58-59 1.0824916199649977 1106 60-61 1.3687149936159209 1106 62-63 1.4021367063992933 1106 64-65 1.3802116220569225 1106 66-67 1.7019734366739279 1106 68-69 1.6931555278832633 1106 70-71 1.6509868611097218 1106 72-73 1.9005869663584356 1106 74-75 2.1812217018336213 1106 76-77 2.3540572603605128 1106 78-79 2.601541402241338 1106 80-81 2.3898809275694823 1106 82-83 2.564010961393219 1106 84-85 2.401758860235411 1106 86-87 2.3448502842420957 1106 88-89 2.0370648556201445 1106 90-91 2.3522667078324027 1106 92-93 2.370954806341894 1106 94-95 2.2173944702366395 1106 96-97 1.9534570935048805 1106 98-99 1.9627265531085207 1106 100-101 1.3144911910308021 1107 1 0.3335673982994045 1107 2 0.2863436086247546 1107 3 0.28561759120919206 1107 4 0.382781302558584 1107 5 0.419864773424635 1107 6 0.329173752160294 1107 7 0.36566196594249334 1107 8 0.40842468346275496 1107 9 0.47818233367330976 1107 10-11 0.49984073727729594 1107 12-13 0.5024757127840402 1107 14-15 0.49784519437091035 1107 16-17 0.5454543354705734 1107 18-19 0.6578729280051689 1107 20-21 0.7353734854835423 1107 22-23 0.6729469125664806 1107 24-25 0.6190831572896016 1107 26-27 0.6518439216815892 1107 28-29 0.74477144517207 1107 30-31 0.773299239066624 1107 32-33 0.9095641405630488 1107 34-35 0.8345197158643671 1107 36-37 0.8965234187853426 1107 38-39 0.9611775873246202 1107 40-41 1.02427544500285 1107 42-43 1.114993229742261 1107 44-45 1.13447910200032 1107 46-47 0.9834246393808712 1107 48-49 1.0407341861491304 1107 50-51 1.3078613726248243 1107 52-53 1.2828909986039143 1107 54-55 1.3774756946406548 1107 56-57 1.6071609102311761 1107 58-59 1.62051033552558 1107 60-61 1.7616909280588438 1107 62-63 1.9190900760323508 1107 64-65 1.8609588111543296 1107 66-67 1.9648402241598113 1107 68-69 2.088812876979098 1107 70-71 2.2392918123909595 1107 72-73 2.146671504733309 1107 74-75 2.23871725946195 1107 76-77 2.2719441376029863 1107 78-79 2.7587985460912634 1107 80-81 2.673380842231328 1107 82-83 2.750520682193823 1107 84-85 2.828359853869646 1107 86-87 2.709641528557288 1107 88-89 2.8011082139178924 1107 90-91 2.787150339461 1107 92-93 2.8427257936791737 1107 94-95 2.7047789836270315 1107 96-97 2.639164862692466 1107 98-99 2.3013637506528735 1107 100-101 1.6669541074318879 1108 1 0.22808598312507655 1108 2 0.16455067494565867 1108 3 0.03498525347674786 1108 4 0.08852545636649012 1108 5 0.07174881823252832 1108 6 0.17193357524711672 1108 7 -0.04525732432610852 1108 8 0.10836648957081962 1108 9 0.21092601884014073 1108 10-11 0.19434518328127837 1108 12-13 0.08592191480158107 1108 14-15 0.21015570724028265 1108 16-17 0.40979430765174385 1108 18-19 0.3542453838950834 1108 20-21 0.29206262288901286 1108 22-23 0.25465549085177486 1108 24-25 0.33410935390443086 1108 26-27 0.2830703989192571 1108 28-29 0.3374386402498857 1108 30-31 0.40011777478096633 1108 32-33 0.4809966045149139 1108 34-35 0.5133716435428894 1108 36-37 0.7116818274281869 1108 38-39 0.6507640642439227 1108 40-41 0.7237264654559254 1108 42-43 0.6835628163877132 1108 44-45 0.6051563562411459 1108 46-47 0.702399031727559 1108 48-49 0.6658574209617214 1108 50-51 0.8074996622393904 1108 52-53 0.8249314740241296 1108 54-55 0.7859566936092683 1108 56-57 1.0554082347400673 1108 58-59 1.1497309727744423 1108 60-61 1.3798506527357226 1108 62-63 1.447700683987641 1108 64-65 1.652088620050833 1108 66-67 1.6557986817735433 1108 68-69 1.8796683365540083 1108 70-71 2.012350053568362 1108 72-73 1.903843983844606 1108 74-75 2.039191846334475 1108 76-77 2.097104451709548 1108 78-79 2.2332820928568182 1108 80-81 2.449776266683905 1108 82-83 2.5978341890289123 1108 84-85 2.59256555406202 1108 86-87 2.6809172482209718 1108 88-89 2.773957743614801 1108 90-91 2.8050007423003755 1108 92-93 2.90668972938405 1108 94-95 2.833284767669168 1108 96-97 2.5415397165639977 1108 98-99 2.3245484219229287 1108 100-101 1.8570625971388708 1109 1 0.564691843148097 1109 2 0.53603014100457 1109 3 0.5066175503511232 1109 4 0.4124217782450543 1109 5 0.45493372603523596 1109 6 0.55107720844493 1109 7 0.495621422008675 1109 8 0.503837102549916 1109 9 0.6129810070795685 1109 10-11 0.5748466555377476 1109 12-13 0.545423548528035 1109 14-15 0.6981916564214643 1109 16-17 0.7454494489276797 1109 18-19 0.8337307780617991 1109 20-21 0.7553466136039688 1109 22-23 0.8932067555113505 1109 24-25 0.7182960440476691 1109 26-27 0.5839937603332856 1109 28-29 0.763134375282803 1109 30-31 0.7431233994897823 1109 32-33 0.9702877513322505 1109 34-35 0.8087363239917522 1109 36-37 0.6775406471341086 1109 38-39 0.8150759090435145 1109 40-41 0.6541624755225968 1109 42-43 0.8938857871883954 1109 44-45 1.2048866396775182 1109 46-47 1.1975368828604687 1109 48-49 1.2958560222409332 1109 50-51 1.1451666988128615 1109 52-53 1.3341732130866042 1109 54-55 1.5405496401483099 1109 56-57 1.7373971848458574 1109 58-59 1.6405896124979051 1109 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-1.5556454967403504 2316 46-47 -1.2040924988296737 2316 48-49 -1.1266102111617258 2316 50-51 -1.1183666193659754 2316 52-53 -1.1503670201449019 2316 54-55 -1.1945846854387518 2316 56-57 -1.252562146662445 2316 58-59 -0.9867013574433372 2316 60-61 -1.2725708722034064 2316 62-63 -1.040469265260434 2316 64-65 -1.0109343914882487 2316 66-67 -1.0577155238981426 2316 68-69 -1.0026047788183767 2316 70-71 -1.1204194535081342 2316 72-73 -0.8208259585521276 2316 74-75 -0.658460268864502 2316 76-77 -0.6173671466334625 2316 78-79 -0.7347392814472364 2316 80-81 -0.7034610879790009 2316 82-83 -0.5580590774226977 2316 84-85 -0.37183821597284705 2316 86-87 -0.3012883932231425 2316 88-89 -0.5568774163499128 2316 90-91 -0.3085275913156771 2316 92-93 -0.32660118861575427 2316 94-95 -0.41528984559607274 2316 96-97 -0.24643541825391857 2316 98-99 -0.42125588572983474 2316 100-101 0.11481621618428584 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 11284.0 3 4547.0 4 4866.0 5 5563.0 6 6726.0 7 8460.0 8 10590.0 9 13720.0 10 17461.0 11 21398.0 12 25669.0 13 30178.0 14 33909.0 15 38656.0 16 43825.0 17 50066.0 18 50553.0 19 51681.0 20 56557.0 21 62833.0 22 69007.0 23 76566.0 24 84657.0 25 93362.0 26 103136.0 27 112776.0 28 124284.0 29 137822.0 30 152346.0 31 169551.0 32 189100.0 33 213394.0 34 238619.0 35 260048.0 36 254329.0 37 179283.0 38 45052.0 39 607.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.70841413592989 18.307662305929895 14.188777008511117 23.795146549629102 2 15.57612318468954 19.66822136358706 40.20057589215933 24.55507955956407 3 19.706950127579695 23.579401892754404 28.97042235335217 27.743225626313727 4 13.012498983982319 15.94626642998917 36.29480637980445 34.74642820622406 5 14.170046989049002 37.134723576281644 33.73491803633374 14.960311398335616 6 33.24153239605035 35.17934944362208 17.166622306775526 14.412495853552047 7 29.541332690123294 31.856692191880214 20.037248939981556 18.564726178014933 8 26.73750190901863 34.83990134060628 19.41873414203623 19.003862608338856 9 26.62873671025957 16.68924636250604 19.606779021135775 37.075237906098614 10-11 25.4131421911124 26.192209620656886 27.037837960070338 21.356810228160377 12-13 26.208197625435385 23.386210668467694 27.386025846845136 23.01956585925178 14-15 22.90997394374516 25.008909066570677 25.522824987592845 26.558292002091317 16-17 22.80098512773008 28.174623589392052 26.221407326264906 22.802983956612962 18-19 22.72732337061597 26.79060860310049 28.346589575979376 22.135478450304163 20-21 23.35338741095937 26.075467913912753 28.361980142989353 22.209164532138523 22-23 22.9904797516323 25.978377118894713 28.250678736145694 22.78046439332729 24-25 22.808653087149484 26.131604127375287 28.36656928931136 22.693173496163872 26-27 22.363283346275665 26.51392913282994 28.20766763896647 22.91511988192792 28-29 22.510429331244737 26.79387774497049 28.168402976906364 22.52728994687841 30-31 22.68173658051885 26.357563785015643 28.085610577625776 22.87508905683973 32-33 22.38062873823721 26.42393821938332 28.031634187232022 23.163798855147448 34-35 22.9580971465883 26.52978963779556 27.896255066118634 22.615858149497505 36-37 23.035768767813188 26.110296018857852 27.9475634786091 22.906371734719862 38-39 22.619128361745634 26.489446508102986 27.847988039408616 23.043437090742763 40-41 23.044501574404947 26.298304563849445 27.93458483004601 22.72260903169959 42-43 22.774607079305614 26.237852952036956 27.80149203215251 23.18604793650492 44-45 22.732526823591392 26.573686735050934 27.834202225044262 22.859584216313408 46-47 23.02462637099425 26.758786601487405 27.314376561941433 22.902210465576918 48-49 22.859267948243268 26.679128562862676 27.358132615982463 23.103470872911593 50-51 23.005015474317485 26.897627052894446 27.247412810755943 22.849944662032126 52-53 23.14446426795784 26.669187243413507 27.244458649081167 22.94188983954749 54-55 22.805035969455247 26.74361555479291 27.39754459221376 23.053803883538087 56-57 22.81144887320928 26.910467607213807 27.23606555926887 23.042017960308044 58-59 22.85404081843931 26.57414950118277 27.29439299718039 23.277416683197536 60-61 22.657569199135857 26.729757250158737 27.533189965003185 23.07948358570222 62-63 22.704019364716466 26.66652250594581 27.61031653435006 23.019141594987662 64-65 22.536604831631372 26.62064286114457 27.491816722095525 23.350935585128532 66-67 22.458913276012034 26.731375629221084 27.34256982654547 23.467141268221408 68-69 22.859477389170397 26.39016456227653 27.47991316083772 23.270444887715353 70-71 22.714178855320966 26.348380632662177 27.32551529494408 23.611925217072777 72-73 22.748807401971064 26.46663346613546 27.249996723631785 23.53456240826169 74-75 22.629107335170083 26.713876304785085 27.13432025034312 23.522696109701712 76-77 22.764682313079618 26.343670685565023 27.22286817705431 23.66877882430105 78-79 22.864000644778066 26.432968472383806 27.134393962675606 23.56863692016252 80-81 22.851098433766992 26.49983749234969 26.976499296892303 23.672564776991013 82-83 22.837809501030442 26.51974548588017 26.99677925618183 23.64566575690756 84-85 22.840854101434456 26.376752526098073 26.979867552880066 23.8025258195874 86-87 22.84848882943148 26.383538168177132 26.859357588089576 23.908615414301806 88-89 22.902195540112043 26.290288361745194 26.76706042711582 24.040455671026947 90-91 22.786071314640964 26.27007771309742 26.841878726541356 24.10197224572026 92-93 22.907331735337248 25.926656435647455 26.933616761397516 24.232395067617777 94-95 22.704750476804 25.838574586629235 27.00880668176034 24.44786825480642 96-97 22.810645720541316 25.634106438200817 26.917555759654743 24.637692081603124 98-99 22.71666692058649 25.635172253476203 26.59297421861907 25.05518660731823 100-101 23.70460683006256 24.722870865941825 26.703932109389378 24.868590194606234 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 266.0 1 209.0 2 186.5 3 243.5 4 230.0 5 255.5 6 427.0 7 878.5 8 1434.0 9 1685.0 10 1547.0 11 1365.0 12 1459.0 13 1705.5 14 1895.0 15 2036.0 16 2252.0 17 2434.5 18 2637.5 19 2984.5 20 3438.5 21 4027.0 22 4883.0 23 6032.0 24 7516.0 25 9561.5 26 12604.0 27 16606.0 28 20368.5 29 24234.5 30 29017.0 31 35425.5 32 43178.5 33 48266.0 34 52152.0 35 57426.5 36 63404.0 37 70159.0 38 75395.5 39 79326.0 40 84425.5 41 90616.5 42 95853.5 43 100313.5 44 105105.5 45 110578.0 46 114909.5 47 119061.5 48 122333.0 49 122295.5 50 120277.5 51 116760.0 52 113430.5 53 111654.5 54 110238.5 55 105113.5 56 96802.0 57 88779.0 58 81026.5 59 70943.0 60 59187.5 61 49727.5 62 41774.0 63 32689.5 64 24455.5 65 18649.0 66 14036.5 67 10577.0 68 8086.5 69 6114.0 70 4642.0 71 3472.5 72 2590.5 73 1984.5 74 1494.0 75 1150.0 76 865.5 77 567.5 78 339.0 79 204.0 80 121.5 81 70.0 82 42.0 83 27.0 84 22.0 85 13.0 86 7.5 87 5.5 88 7.0 89 6.5 90 5.0 91 4.0 92 2.0 93 0.5 94 1.0 95 1.0 96 0.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3811325934543081 2 0.028435885432210716 3 0.03456204969007178 4 0.04432460021864182 5 0.06496354932266572 6 0.05493891690071126 7 0.03665870483714723 8 0.03849327809083824 9 0.034955172530148425 10-11 0.03736304992561788 12-13 0.0291566106390179 14-15 0.026683212770202337 16-17 0.022735604251099353 18-19 0.02742031809534605 20-21 0.03488965205680232 22-23 0.03398874554829334 24-25 0.04234260589992206 26-27 0.03683888613884902 28-29 0.03246539454299634 30-31 0.034693090636764 32-33 0.029779055135805924 34-35 0.03140068685112209 36-37 0.033415441406514895 38-39 0.015004188396258649 40-41 0.01230146887073171 42-43 0.013169615142567636 44-45 0.010384995025358062 46-47 0.019328539637101756 48-49 0.016740480939930504 50-51 0.0107125973920886 52-53 0.008828883783388005 54-55 0.007813316446523336 56-57 0.008206439286599981 58-59 0.0036200061523724474 60-61 0.006650328044629925 62-63 0.010876398575453868 64-65 0.00720725206807184 66-67 0.0031449827206131665 68-69 0.005618380589428731 70-71 0.012907533249183206 72-73 0.010516035972050277 74-75 0.011466082835568837 76-77 0.004979555974304181 78-79 0.008779743428378424 80-81 0.010384995025358062 82-83 0.012809252539164043 84-85 0.007993497748225132 86-87 0.0020475147920658637 88-89 0.01633097798151733 90-91 0.026453891113490956 92-93 0.010647076918742492 94-95 0.02131053395582151 96-97 0.019263019163755647 98-99 0.01102381964048261 100-101 0.12488202219768116 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3052481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.90539573194361 #Duplication Level Percentage of deduplicated Percentage of total 1 75.9272304640146 41.68814635457206 2 13.127171149277695 14.415050535840896 3 4.637459933502611 7.6386471851998134 4 2.104100714971465 4.621059296614951 5 1.1358991876825157 3.1183497205650905 6 0.6991396651698141 2.303192399282832 7 0.46049555887899735 1.769858362513772 8 0.3156075838665452 1.3862847430556196 9 0.23283419903679742 1.1505468454240937 >10 1.2363971785751047 12.261813694004035 >50 0.07828447429732505 2.9745434210084696 >100 0.04092589780485263 3.8798325906023092 >500 0.002828887396569318 1.0588608492829477 >1k 0.0015047273379477553 1.3368196704516608 >5k 1.2037818703582042E-4 0.39699433158152386 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6728 0.2204108723363061 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5285 0.17313785081708943 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3509 0.11495567048574586 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 1.3104094669221528E-4 14-15 0.0 0.0 0.0 0.0 1.3104094669221528E-4 16-17 0.0 0.0 0.0 3.276023667305382E-5 1.3104094669221528E-4 18-19 0.0 0.0 0.0 3.276023667305382E-5 1.638011833652691E-4 20-21 0.0 0.0 0.0 6.552047334610764E-5 1.9656142003832293E-4 22-23 0.0 0.0 0.0 2.948421300574844E-4 2.6208189338443056E-4 24-25 0.0 0.0 0.0 5.896842601149688E-4 2.6208189338443056E-4 26-27 0.0 0.0 0.0 0.0011957486385664644 3.4398248506706513E-4 28-29 0.0 0.0 0.0 0.003898468164093405 3.6036260340359204E-4 30-31 3.276023667305382E-5 0.0 0.0 0.012514410409106561 3.6036260340359204E-4 32-33 3.276023667305382E-5 0.0 0.0 0.03253091501634244 3.6036260340359204E-4 34-35 3.276023667305382E-5 1.638011833652691E-5 0.0 0.0631781164239843 3.6036260340359204E-4 36-37 3.276023667305382E-5 3.276023667305382E-5 0.0 0.11339955924377579 3.6036260340359204E-4 38-39 3.276023667305382E-5 3.276023667305382E-5 0.0 0.19272847234757562 3.6036260340359204E-4 40-41 3.276023667305382E-5 3.276023667305382E-5 0.0 0.276021374088815 3.6036260340359204E-4 42-43 4.914035500958073E-5 3.276023667305382E-5 0.0 0.362475638669004 3.6036260340359204E-4 44-45 6.552047334610764E-5 3.276023667305382E-5 0.0 0.47292677661220495 3.6036260340359204E-4 46-47 6.552047334610764E-5 3.276023667305382E-5 0.0 0.6004623779804035 3.7674272174011896E-4 48-49 6.552047334610764E-5 3.276023667305382E-5 0.0 0.7373346468004223 4.258830767496997E-4 50-51 6.552047334610764E-5 3.276023667305382E-5 0.0 0.8881791565614987 4.258830767496997E-4 52-53 6.552047334610764E-5 3.276023667305382E-5 0.0 1.0336837477448673 4.258830767496997E-4 54-55 6.552047334610764E-5 3.276023667305382E-5 0.0 1.192718316674207 4.258830767496997E-4 56-57 6.552047334610764E-5 3.276023667305382E-5 0.0 1.379271484408912 4.258830767496997E-4 58-59 6.552047334610764E-5 3.276023667305382E-5 0.0 1.5838100220771234 4.258830767496997E-4 60-61 6.552047334610764E-5 3.276023667305382E-5 0.0 1.8029760054198536 4.258830767496997E-4 62-63 6.552047334610764E-5 3.276023667305382E-5 0.0 2.0224368308926413 4.258830767496997E-4 64-65 6.552047334610764E-5 6.552047334610764E-5 0.0 2.2521516104440944 4.258830767496997E-4 66-67 6.552047334610764E-5 6.552047334610764E-5 0.0 2.5165103402773026 4.258830767496997E-4 68-69 6.552047334610764E-5 6.552047334610764E-5 0.0 2.811647312464844 4.258830767496997E-4 70-71 6.552047334610764E-5 6.552047334610764E-5 0.0 3.1236230463023356 4.258830767496997E-4 72-73 6.552047334610764E-5 6.552047334610764E-5 0.0 3.438465300848719 4.5864331342275347E-4 74-75 8.190059168263455E-5 6.552047334610764E-5 0.0 3.763872731722163 4.914035500958073E-4 76-77 9.828071001916147E-5 6.552047334610764E-5 0.0 4.1096897900429195 4.914035500958073E-4 78-79 9.828071001916147E-5 9.828071001916147E-5 0.0 4.49798049521029 4.914035500958073E-4 80-81 9.828071001916147E-5 1.3104094669221528E-4 0.0 4.90419104983782 4.914035500958073E-4 82-83 9.828071001916147E-5 1.3104094669221528E-4 0.0 5.323489319016236 5.077836684323342E-4 84-85 9.828071001916147E-5 1.3104094669221528E-4 0.0 5.740920254704288 5.241637867688611E-4 86-87 9.828071001916147E-5 1.3104094669221528E-4 0.0 6.173617460681983 5.733041417784418E-4 88-89 9.828071001916147E-5 1.3104094669221528E-4 0.0 6.625463024995078 6.224444967880226E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 47.646465 1 GTATCAA 8795 0.0 43.003765 1 ATCAACG 11085 0.0 33.483395 3 TATCAAC 11255 0.0 32.977646 2 TCAACGC 11410 0.0 32.61296 4 CAACGCA 11380 0.0 32.573654 5 AACGCAG 11920 0.0 31.289139 6 ACGCAGA 13730 0.0 26.91771 7 CGCAGAG 13735 0.0 26.769567 8 GTACATG 11790 0.0 24.717045 1 TACATGG 11570 0.0 24.39873 2 GCAGAGT 15370 0.0 23.241213 9 ACATGGG 12015 0.0 22.7831 3 GAGTACT 7640 0.0 21.48139 12-13 TGGTATC 2505 0.0 21.248312 2 AGAGTAC 13570 0.0 20.49482 10-11 GTGGTAT 2860 0.0 19.844954 1 GTACTTT 8150 0.0 19.671852 14-15 CAGAGTA 14680 0.0 19.527573 10-11 AGTACTT 8330 0.0 19.188797 12-13 >>END_MODULE