FastQCFastQC Report
Thu 26 May 2016
SRR938452_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938452_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3052481
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT68450.2242438200270534No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57330.18781443684661756No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39600.12973053722529312No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA53300.048.0482641
GTATCAA90200.039.0853041
ATCAACG117650.029.8355083
TATCAAC118850.029.6565932
TCAACGC119000.029.4960734
CAACGCA120850.029.0445385
AACGCAG124900.028.1407726
GTACATG117900.024.6231461
TACATGG115700.024.141292
ACGCAGA145400.024.0425267
CGCAGAG145550.023.9198518
ACATGGG115200.023.501923
GCAGAGT162650.020.7042229
ACCGTAT1856.5614677E-620.539278
GTACCGT2551.1947122E-820.488936
GAGTACT80450.019.98478112-13
AGAGTAC143600.019.81249210-11
AGTACTT86650.019.65112112-13
TAAGGTG15400.019.1221925
CATGGGG64350.018.8954894
Produced by FastQC (version 0.11.5)