##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938452_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3052481 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.7934575186545 31.0 31.0 34.0 30.0 34.0 2 32.05193807922146 31.0 31.0 34.0 30.0 34.0 3 32.11298645265933 33.0 31.0 34.0 30.0 34.0 4 35.768518788487135 37.0 35.0 37.0 35.0 37.0 5 35.661200184374614 37.0 35.0 37.0 33.0 37.0 6 35.68706602923982 37.0 35.0 37.0 33.0 37.0 7 35.60841820145645 37.0 35.0 37.0 33.0 37.0 8 35.57598753276433 37.0 35.0 37.0 33.0 37.0 9 37.11632406557158 39.0 37.0 39.0 33.0 39.0 10-11 37.04410232201282 39.0 37.0 39.0 33.0 39.0 12-13 36.925487496891876 39.0 37.0 39.0 33.0 39.0 14-15 37.94864603579842 40.0 37.0 41.0 33.0 41.0 16-17 38.03648654979343 40.0 37.0 41.0 33.0 41.0 18-19 38.1595351453457 40.0 37.0 41.0 33.0 41.0 20-21 38.10822344184943 40.0 37.0 41.0 33.0 41.0 22-23 37.98634553335467 40.0 37.0 41.0 33.0 41.0 24-25 37.87262607039979 40.0 37.0 41.0 32.0 41.0 26-27 37.665196441845175 40.0 37.0 41.0 32.0 41.0 28-29 37.52483553542184 39.5 36.5 41.0 32.0 41.0 30-31 37.278538343072405 39.0 36.0 41.0 31.0 41.0 32-33 37.19869771507177 39.0 36.0 41.0 31.0 41.0 34-35 37.0777859059565 39.0 36.0 41.0 31.0 41.0 36-37 36.96888989644817 39.0 36.0 41.0 30.5 41.0 38-39 36.76316298119464 39.0 35.0 40.5 30.0 41.0 40-41 36.549625861717075 39.0 35.0 40.0 30.0 41.0 42-43 36.29923691580717 39.0 35.0 40.0 30.0 41.0 44-45 36.0252130971495 38.0 35.0 40.0 29.5 41.0 46-47 35.640691948614915 38.0 34.5 40.0 28.0 41.0 48-49 35.248259366724966 38.0 34.0 40.0 27.0 41.0 50-51 34.866389995547884 38.0 33.5 40.0 26.0 41.0 52-53 34.27070864650754 37.5 33.0 40.0 24.0 41.0 54-55 33.84933010230039 37.0 33.0 40.0 23.5 41.0 56-57 33.45886870385107 37.0 32.0 39.5 23.0 40.5 58-59 32.92739184944968 36.0 31.0 39.0 21.5 40.0 60-61 32.441079895337595 36.0 31.0 39.0 19.5 40.0 62-63 31.927295698155042 35.0 30.5 38.5 18.0 40.0 64-65 31.271075888760652 35.0 29.5 38.0 13.5 40.0 66-67 30.62571085618551 34.0 29.0 38.0 8.0 40.0 68-69 29.903134532205115 34.0 28.0 37.0 7.5 39.0 70-71 29.130337584410846 33.5 26.5 36.0 4.0 39.0 72-73 28.545738532033454 33.0 26.0 36.0 2.0 39.0 74-75 27.827373536477378 32.5 26.0 35.0 2.0 38.0 76-77 26.68339049448629 31.0 24.5 34.0 2.0 36.0 78-79 27.1922555783312 32.0 25.5 35.0 2.0 37.0 80-81 26.817785434209092 32.0 25.0 35.0 2.0 36.0 82-83 26.14141693265249 31.5 23.5 34.5 2.0 36.0 84-85 25.36780032373666 31.0 20.5 34.0 2.0 35.0 86-87 24.476576758381132 31.0 17.5 34.0 2.0 35.0 88-89 23.517056617223822 30.0 9.0 34.0 2.0 35.0 90-91 22.66987411223854 29.5 2.0 34.0 2.0 35.0 92-93 21.59577193109474 29.0 2.0 34.0 2.0 35.0 94-95 20.425790856683463 27.5 2.0 33.0 2.0 34.5 96-97 19.06458795320921 25.5 2.0 33.0 2.0 34.0 98-99 17.303783545253843 21.0 2.0 32.0 2.0 34.0 100-101 13.021789652417166 2.0 2.0 27.5 2.0 32.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3322094422612665 1101 2 0.36910195353959807 1101 3 0.2676377271042796 1101 4 0.1711232916639105 1101 5 0.12153343836573782 1101 6 0.1774416054080632 1101 7 0.25148103362224816 1101 8 0.2449848567214019 1101 9 0.29921887650612433 1101 10-11 0.3196037918365633 1101 12-13 0.37257124503467764 1101 14-15 0.5163971670036673 1101 16-17 0.3861312725076971 1101 18-19 0.40528496279409154 1101 20-21 0.4491225954740301 1101 22-23 0.4200832582201457 1101 24-25 0.25066484851309667 1101 26-27 0.324841445120434 1101 28-29 0.40110508579917337 1101 30-31 0.45950119460938055 1101 32-33 0.47704573709681597 1101 34-35 0.3326709814118729 1101 36-37 0.41502851234103133 1101 38-39 0.5483128921364298 1101 40-41 0.5121815573653734 1101 42-43 0.5778828574529129 1101 44-45 0.6863161177952932 1101 46-47 0.6601038725366735 1101 48-49 0.6865383252337978 1101 50-51 0.8120596842546703 1101 52-53 0.889197253719658 1101 54-55 0.862862207638976 1101 56-57 1.0856364616595826 1101 58-59 1.1208697800226943 1101 60-61 1.0022735642255682 1101 62-63 1.0434831810969563 1101 64-65 1.2519762278096316 1101 66-67 1.2440802084095317 1101 68-69 1.2996845988192725 1101 70-71 1.5359456104216491 1101 72-73 1.5858888131031037 1101 74-75 1.709369864758873 1101 76-77 1.8355827209155677 1101 78-79 1.7897943984778344 1101 80-81 1.8894585199127718 1101 82-83 2.0670512370977896 1101 84-85 2.17304535028741 1101 86-87 2.1714196751062005 1101 88-89 2.1643949125859514 1101 90-91 2.23114969045238 1101 92-93 2.4444553320763376 1101 94-95 2.350732546226368 1101 96-97 2.5148866569652206 1101 98-99 2.31516204676376 1101 100-101 2.136968571139098 1102 1 0.28233225822950914 1102 2 0.3324212355671392 1102 3 0.3124304975466785 1102 4 0.24717260682016473 1102 5 0.2957591158097159 1102 6 0.3302494641629181 1102 7 0.34091216052279094 1102 8 0.28694817123530214 1102 9 0.3671485590918593 1102 10-11 0.42460762406643227 1102 12-13 0.45164453128091253 1102 14-15 0.6012999784996467 1102 16-17 0.4792109341075559 1102 18-19 0.44106970065281104 1102 20-21 0.5185614516319603 1102 22-23 0.5663690517301418 1102 24-25 0.4589043322544484 1102 26-27 0.3689542683154414 1102 28-29 0.27827255137176365 1102 30-31 0.3223353012963557 1102 32-33 0.3401773627312963 1102 34-35 0.4181773278510832 1102 36-37 0.3167404658295325 1102 38-39 0.5186663241064409 1102 40-41 0.5877343148042371 1102 42-43 0.5041784830869034 1102 44-45 0.4394050393438178 1102 46-47 0.46631309969529156 1102 48-49 0.5923100338777232 1102 50-51 0.6466553488528248 1102 52-53 0.4718242011483582 1102 54-55 0.5403710467086782 1102 56-57 0.7474878083072483 1102 58-59 0.9975448447072424 1102 60-61 0.8962531427010632 1102 62-63 0.8878896353814234 1102 64-65 1.0460961726313904 1102 66-67 1.0704794002241016 1102 68-69 1.3530853681505768 1102 70-71 1.593086975996325 1102 72-73 1.651713061624772 1102 74-75 1.6917557894412845 1102 76-77 1.7228061679760849 1102 78-79 1.7790936194024347 1102 80-81 1.9323519861228213 1102 82-83 1.917655768457461 1102 84-85 1.9554609473957747 1102 86-87 1.8221014990397784 1102 88-89 1.6573929169410881 1102 90-91 1.8259343245126303 1102 92-93 2.253397980179585 1102 94-95 2.144643610373457 1102 96-97 2.3031726751885166 1102 98-99 2.213798741223723 1102 100-101 1.8062795925686537 1103 1 0.24973614731539584 1103 2 0.29592418372025975 1103 3 0.30456758675448725 1103 4 0.08934022802834818 1103 5 0.2347894643725752 1103 6 0.21131180240556802 1103 7 0.23413012814027923 1103 8 0.24966125075142998 1103 9 0.3642391628468218 1103 10-11 0.4005527569569267 1103 12-13 0.3846228446167501 1103 14-15 0.5079634253643164 1103 16-17 0.4640850549315019 1103 18-19 0.4186587564125617 1103 20-21 0.46358438482948117 1103 22-23 0.420739652342462 1103 24-25 0.498796556572934 1103 26-27 0.5330747309988837 1103 28-29 0.5065779078525949 1103 30-31 0.5326357214489548 1103 32-33 0.5604419827543268 1103 34-35 0.5282570267189257 1103 36-37 0.6374448448654633 1103 38-39 0.6583808279815955 1103 40-41 0.6098397636702373 1103 42-43 0.6357747367525874 1103 44-45 0.6353356024549228 1103 46-47 0.8170884301195827 1103 48-49 0.7927854983201286 1103 50-51 0.9126821938642635 1103 52-53 0.9897325083427191 1103 54-55 0.9251095009301409 1103 56-57 1.0982205237051588 1103 58-59 1.1020656501347403 1103 60-61 1.0885728276515039 1103 62-63 1.2520906281887783 1103 64-65 1.4503577454169836 1103 66-67 1.3713586727312368 1103 68-69 1.399103107926738 1103 70-71 1.6176460332326918 1103 72-73 1.660777786308902 1103 74-75 1.5816929261394144 1103 76-77 1.6728380327921002 1103 78-79 1.617625284471476 1103 80-81 1.8063041036687437 1103 82-83 1.8482348382713987 1103 84-85 1.9729529250732156 1103 86-87 2.0302324109978365 1103 88-89 2.023431967737846 1103 90-91 2.2596156980984006 1103 92-93 2.4616160512656577 1103 94-95 2.094927131826701 1103 96-97 2.0966406158866384 1103 98-99 2.3847304179373623 1103 100-101 2.121852280650863 1104 1 0.17203270376936786 1104 2 0.20732091010184917 1104 3 0.2824000049249449 1104 4 0.15797536853533956 1104 5 0.16520803671848938 1104 6 0.1551238598402307 1104 7 0.20762622504707196 1104 8 0.18554677457842672 1104 9 0.18248650581666936 1104 10-11 0.28715304701331945 1104 12-13 0.228339399672862 1104 14-15 0.3390709362523623 1104 16-17 0.3320611252789121 1104 18-19 0.4467602084418658 1104 20-21 0.4004195266469637 1104 22-23 0.3703966846811326 1104 24-25 0.4119844030352553 1104 26-27 0.49622976046320133 1104 28-29 0.41675882179234236 1104 30-31 0.44322037729092045 1104 32-33 0.5802202577924405 1104 34-35 0.5287502099568115 1104 36-37 0.4684270395963992 1104 38-39 0.4985613378518181 1104 40-41 0.5471230404338314 1104 42-43 0.533906024402043 1104 44-45 0.5410852933963426 1104 46-47 0.6031420250278714 1104 48-49 0.629328945622909 1104 50-51 0.6370952009715083 1104 52-53 0.7602018514785627 1104 54-55 0.9076886439532714 1104 56-57 0.9027074973772997 1104 58-59 0.8607763516431177 1104 60-61 0.8044802258369117 1104 62-63 0.9983408629859376 1104 64-65 1.0447654777787996 1104 66-67 1.0933397819348478 1104 68-69 1.3435516840730948 1104 70-71 1.4562070979668142 1104 72-73 1.64229826717348 1104 74-75 1.5099510370160907 1104 76-77 1.6028852434018752 1104 78-79 1.7306909272041189 1104 80-81 1.9548888774553355 1104 82-83 2.114948955805392 1104 84-85 2.2441759124793528 1104 86-87 2.185282843243023 1104 88-89 2.081165312428812 1104 90-91 2.1265260077685504 1104 92-93 2.4413158712909038 1104 94-95 2.3631844673267217 1104 96-97 2.421499846800362 1104 98-99 2.5100582602934587 1104 100-101 2.1851642419825 1105 1 0.12633122215239467 1105 2 0.11811293497838449 1105 3 0.08565133106360179 1105 4 0.08560020848411654 1105 5 0.09237101458569441 1105 6 -0.018380804509504856 1105 7 0.07589950106682863 1105 8 0.08024221997810344 1105 9 0.18994031960851032 1105 10-11 0.18927486885345246 1105 12-13 0.17672288238733103 1105 14-15 0.22824918757849844 1105 16-17 0.15115294894820153 1105 18-19 0.16631160774678477 1105 20-21 0.18811616099853268 1105 22-23 0.19558645969484445 1105 24-25 0.1977444176685097 1105 26-27 0.23886511806334454 1105 28-29 0.25047932663960637 1105 30-31 0.29283625437158634 1105 32-33 0.34494268325430255 1105 34-35 0.34669423775999775 1105 36-37 0.2882002241583166 1105 38-39 0.31921480410387204 1105 40-41 0.433390646067636 1105 42-43 0.3647478938002422 1105 44-45 0.5281897382472565 1105 46-47 0.5195541340456131 1105 48-49 0.47210606287185897 1105 50-51 0.4080481916428127 1105 52-53 0.40943131919394915 1105 54-55 0.5287102280030282 1105 56-57 0.7828771516666677 1105 58-59 0.5187811989085347 1105 60-61 0.6217863352204773 1105 62-63 0.7194493319817319 1105 64-65 0.7709841534693034 1105 66-67 0.8140572686734622 1105 68-69 0.9955934803050361 1105 70-71 1.0298466626275058 1105 72-73 1.2517184244809592 1105 74-75 1.3412250446985503 1105 76-77 1.2074924319879337 1105 78-79 1.1691260835970247 1105 80-81 1.398418235421314 1105 82-83 1.5767218659938003 1105 84-85 1.8359607121866865 1105 86-87 1.6738323219333466 1105 88-89 1.561273232677575 1105 90-91 1.5992807178472042 1105 92-93 1.6919949457375871 1105 94-95 1.5359643283108042 1105 96-97 1.8671601723902604 1105 98-99 1.9052064219909148 1105 100-101 1.674795209606426 1106 1 0.04980947978637573 1106 2 0.10770355941895104 1106 3 0.058694303135617076 1106 4 0.09472721049382926 1106 5 0.10285129433268736 1106 6 0.02909657746810268 1106 7 0.08327756756958138 1106 8 -0.0011259166309116608 1106 9 0.009642236750245559 1106 10-11 0.13542546397317068 1106 12-13 0.13863932335267037 1106 14-15 0.12673552787642706 1106 16-17 0.018552660866347992 1106 18-19 0.1405029485883844 1106 20-21 0.02415725565996496 1106 22-23 0.11975954735930117 1106 24-25 0.11849394934944968 1106 26-27 -0.10716297611892145 1106 28-29 0.20907050591060994 1106 30-31 0.25649568770862885 1106 32-33 0.15072748305022543 1106 34-35 0.04430317491242164 1106 36-37 -0.013182624552818822 1106 38-39 0.2160134490276917 1106 40-41 0.2264380667055903 1106 42-43 0.29923500846285833 1106 44-45 0.3105516600938998 1106 46-47 -0.1505872857001478 1106 48-49 0.049953796232422576 1106 50-51 0.3775828534546477 1106 52-53 0.24964382297171284 1106 54-55 0.1836280742356209 1106 56-57 0.4547523648443459 1106 58-59 0.5118963609525125 1106 60-61 0.48444538902374035 1106 62-63 0.3581878451932674 1106 64-65 0.6390063958855521 1106 66-67 0.48366520159107296 1106 68-69 0.414046119408507 1106 70-71 0.44153270632681085 1106 72-73 0.7971995331661255 1106 74-75 0.66980001109658 1106 76-77 0.6022319614239215 1106 78-79 1.031986273769082 1106 80-81 0.998831381512467 1106 82-83 1.326695922916425 1106 84-85 1.2840679424706707 1106 86-87 1.3347081354193655 1106 88-89 1.3202975476459677 1106 90-91 1.314490585973683 1106 92-93 1.1888029136318394 1106 94-95 0.8854897250331746 1106 96-97 0.8326354661051774 1106 98-99 1.1125366746785552 1106 100-101 1.1420530304623462 1107 1 0.10457110697423389 1107 2 0.12975239179655773 1107 3 0.10490503734285284 1107 4 0.12788935091492704 1107 5 0.110598063994793 1107 6 0.10017501779264393 1107 7 0.11942530948449104 1107 8 0.1465779934360043 1107 9 0.1960809822665368 1107 10-11 0.1331353511659117 1107 12-13 0.17775193684526158 1107 14-15 0.32918680097461106 1107 16-17 0.23719498560996044 1107 18-19 0.20868877590007884 1107 20-21 0.23206316197831 1107 22-23 0.24385445207423828 1107 24-25 0.3250074469433457 1107 26-27 0.3346836369138586 1107 28-29 0.24475266378037475 1107 30-31 0.24678475849211878 1107 32-33 0.3915556550457495 1107 34-35 0.4023092708897664 1107 36-37 0.3420828777967202 1107 38-39 0.4470083853014657 1107 40-41 0.43334563013395666 1107 42-43 0.4526169412748331 1107 44-45 0.5466085457477803 1107 46-47 0.5882205585396036 1107 48-49 0.6170455281843417 1107 50-51 0.842122258902883 1107 52-53 0.827048490776285 1107 54-55 0.7870495765089487 1107 56-57 0.8331648915575585 1107 58-59 0.8945682437925484 1107 60-61 0.9683130721407096 1107 62-63 1.2102783141010143 1107 64-65 1.1906832141237764 1107 66-67 1.0873337847220341 1107 68-69 1.293159860213681 1107 70-71 1.185147719405908 1107 72-73 1.3502987197933152 1107 74-75 1.2686771702530741 1107 76-77 1.4482476271202671 1107 78-79 1.5245178279096514 1107 80-81 1.719930645531342 1107 82-83 2.0122559174160877 1107 84-85 1.951195043608081 1107 86-87 2.185419423263429 1107 88-89 2.3054657368591656 1107 90-91 2.411986885930439 1107 92-93 2.2811250265196605 1107 94-95 1.9863013802212244 1107 96-97 1.9668146359549539 1107 98-99 2.0254385006584137 1107 100-101 1.857095012984658 1108 1 -0.022709843117130646 1108 2 0.015243385788494379 1108 3 0.02139535107956192 1108 4 0.011939589612879331 1108 5 0.029328678710498934 1108 6 0.03822085472895509 1108 7 0.005627458117217543 1108 8 0.03888998573174973 1108 9 -0.008221713988028512 1108 10-11 0.07943193963676975 1108 12-13 -0.046081107079196215 1108 14-15 0.03217046689017167 1108 16-17 -0.07805701079634275 1108 18-19 -0.13043222010967526 1108 20-21 -0.08000722500872826 1108 22-23 0.009991412311840975 1108 24-25 0.03625450506186212 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-0.6521074095985426 2316 50-51 -0.665430674880497 2316 52-53 -0.6053064792835485 2316 54-55 -0.3821349234080813 2316 56-57 -0.43648600350626765 2316 58-59 -0.5200246777810165 2316 60-61 -0.4538607042912801 2316 62-63 -0.43880956757702094 2316 64-65 -0.454506281779139 2316 66-67 -0.3007308612448689 2316 68-69 -0.05318999217722009 2316 70-71 -0.19311865727146582 2316 72-73 0.06169323505656976 2316 74-75 0.23670414352081792 2316 76-77 0.20275025420101045 2316 78-79 0.11799843610990379 2316 80-81 -0.2511481201043395 2316 82-83 0.12807376296367678 2316 84-85 0.2652268647668059 2316 86-87 0.3086650765604624 2316 88-89 0.29951472759248077 2316 90-91 0.2671749376723369 2316 92-93 0.37357618656957925 2316 94-95 0.6803393157180722 2316 96-97 0.24476988130149735 2316 98-99 0.3103464710028341 2316 100-101 0.0183859608683683 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 10.0 3 18.0 4 51.0 5 100.0 6 193.0 7 580.0 8 1252.0 9 2354.0 10 3918.0 11 6029.0 12 9003.0 13 12277.0 14 16598.0 15 22433.0 16 27729.0 17 34320.0 18 35481.0 19 34257.0 20 36360.0 21 40148.0 22 44723.0 23 49064.0 24 56364.0 25 64145.0 26 73825.0 27 86283.0 28 101182.0 29 119182.0 30 138217.0 31 161273.0 32 189701.0 33 226228.0 34 272627.0 35 331120.0 36 386478.0 37 347212.0 38 119143.0 39 2603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.490205613307516 19.14660057121361 14.84860479960993 23.51458901586894 2 16.0855842883162 20.2846584488503 39.32783098072866 24.301926282104837 3 19.69820765750566 23.69590785046668 29.512912663025855 27.09297182900181 4 13.522410131299752 16.661233927418383 36.02777543906088 33.78858050222098 5 14.067455513909879 37.226496173100415 33.92687432575117 14.779173987238536 6 33.24947804752921 35.72507085220186 16.797713073398327 14.2277380268706 7 29.535548296615115 31.951648511489505 19.963007140748786 18.54979605114659 8 26.685294149443777 35.37482157944412 19.16321127843959 18.776672992672513 9 26.357245116250116 16.725766346598 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34-35 23.002285096645224 26.54665035695113 27.961382210713463 22.48968233569018 36-37 23.070539799708236 26.165515732132288 28.041158454184462 22.722786013975018 38-39 22.583987134060123 26.511607867331545 28.001210318373694 22.90319468023464 40-41 23.03048333373743 26.304907384897056 28.059162004839955 22.60544727652556 42-43 22.74894846499724 26.30738517722646 27.894864354974167 23.048802002802127 44-45 22.750204302398018 26.57230126319407 27.983376033858587 22.694118400549325 46-47 23.00136659394437 26.78399641995958 27.419971207013837 22.79466577908222 48-49 22.782074646820078 26.721951750068225 27.485412685615408 23.010560917496292 50-51 22.975933347332873 26.88123202077261 27.38495341985749 22.757881212037027 52-53 23.213019180135767 26.670272476716484 27.289506470310542 22.82720187283721 54-55 22.811608000180836 26.757250905083442 27.450965296753687 22.980175797982035 56-57 22.82366376727652 26.945409324415127 27.27325739292071 22.957669515387646 58-59 22.803401560894237 26.658806393880912 27.31458443148377 23.223207613741085 60-61 22.646316226046945 26.774368128745106 27.570015996823567 23.009299648384378 62-63 22.637487342263555 26.8208221443475 27.591621372909447 22.9500691404795 64-65 22.446313307970343 26.757530518637473 27.45715859142684 23.338997581965344 66-67 22.439125388252045 26.846566199164414 27.327423788593013 23.38688462399053 68-69 22.849306532798312 26.562158317666963 27.400255171949496 23.18827997758523 70-71 22.65822303773425 26.54182059892959 27.23399571299262 23.56596065034354 72-73 22.748179755771954 26.61011146692438 27.111819097617506 23.529889679686157 74-75 22.562794002648992 26.947293038023822 26.93361563921282 23.556297320114357 76-77 22.54100844526141 26.538854787302522 27.30339681065992 23.616739956776144 78-79 22.669477713374793 26.710289105812617 27.185787561003654 23.434445619808937 80-81 22.69021783641728 26.801227463362505 27.051745567693615 23.456809132526597 82-83 22.650499115219358 26.76289860788943 27.113452908260786 23.473149368630427 84-85 22.553255061608148 26.746350434612676 27.040373120013317 23.660021383765866 86-87 22.569090439709917 26.676808492793814 26.9291692766553 23.82493179084097 88-89 22.50158150042539 26.739593137516664 26.766227209931852 23.992598152126092 90-91 22.435379250957716 26.62813361553339 26.984192505383795 23.9522946281251 92-93 22.412229075340143 26.45715934967926 27.002735168288687 24.127876406691907 94-95 22.26727120243212 26.27712548485166 27.072446011112277 24.38315730160394 96-97 22.26309062416615 26.144589621457044 27.089755364530582 24.502564389846228 98-99 22.093798454437554 26.2342173464798 26.830699355704425 24.841284843378222 100-101 22.406473163865638 25.918093798828135 26.982179569101117 24.69325346820511 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 311.0 1 234.5 2 196.0 3 256.0 4 245.5 5 247.5 6 447.0 7 974.5 8 1571.0 9 1807.0 10 1708.5 11 1554.0 12 1564.0 13 1736.0 14 1897.0 15 2045.5 16 2266.5 17 2497.0 18 2754.5 19 3062.5 20 3404.0 21 4052.0 22 4888.0 23 5924.0 24 7362.0 25 9276.5 26 12396.0 27 16455.5 28 20230.5 29 24489.5 30 29333.0 31 35728.5 32 44596.0 33 49702.5 34 52594.5 35 57960.5 36 64535.5 37 71088.5 38 76309.5 39 80598.0 40 85254.0 41 91356.0 42 96387.5 43 101801.0 44 107454.0 45 112792.5 46 116736.0 47 121108.5 48 124655.5 49 124075.0 50 121830.5 51 117733.5 52 113849.5 53 112965.5 54 113023.5 55 106744.0 56 96699.0 57 87939.0 58 79528.0 59 68967.5 60 56760.0 61 46951.0 62 38781.5 63 29268.0 64 21058.0 65 15710.5 66 11488.5 67 8581.5 68 6539.5 69 4948.0 70 3697.5 71 2598.5 72 1895.5 73 1547.5 74 1173.5 75 884.5 76 644.5 77 388.5 78 228.5 79 137.0 80 67.5 81 32.5 82 23.0 83 15.5 84 9.0 85 3.5 86 0.5 87 0.0 88 0.5 89 1.5 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.024013253481348452 2 0.010876398575453868 3 0.001670772070325745 4 0.0 5 3.276023667305382E-4 6 0.0 7 0.0 8 6.552047334610764E-5 9 3.6036260340359204E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0018181931353544872 26-27 0.00891078437507064 28-29 1.8018130170179602E-4 30-31 0.0 32-33 1.474210650287422E-4 34-35 0.01023757396032932 36-37 0.00111384804688383 38-39 0.012825632657500571 40-41 0.012678211592471829 42-43 0.005159737276005977 44-45 6.715848517976033E-4 46-47 4.914035500958073E-5 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.552047334610764E-5 66-67 5.896842601149688E-4 68-69 9.664269818550876E-4 70-71 6.552047334610764E-5 72-73 1.965614200383229E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.2932165671137676E-4 82-83 9.664269818550876E-4 84-85 0.0054218191693904075 86-87 0.003439824850670651 88-89 0.0 90-91 0.0011957486385664644 92-93 5.896842601149688E-4 94-95 3.276023667305382E-5 96-97 1.638011833652691E-5 98-99 0.0 100-101 4.914035500958073E-5 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 3052481.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.61073148698995 #Duplication Level Percentage of deduplicated Percentage of total 1 73.18935813373143 37.773563103451565 2 14.524611254437147 14.992516228113356 3 5.124754610362526 7.934770023964023 4 2.375942321380155 4.90496484709307 5 1.3224931197215364 3.412741864766993 6 0.7645319196324039 2.3674830970428586 7 0.521184008785276 1.882908155291092 8 0.3530286321548012 1.457605275308863 9 0.2593401008716375 1.2046259078905475 >10 1.415913116494791 13.23788775764016 >50 0.09676524476136938 3.427931671484981 >100 0.04717403350394982 4.391888304957399 >500 0.0031204117403974374 1.1290690612106664 >1k 0.0016557286781732378 1.4686475996585113 >5k 1.2736374447486445E-4 0.4133971021260995 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6845 0.2242438200270534 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5733 0.18781443684661756 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3960 0.12973053722529312 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.276023667305382E-5 14-15 0.0 0.0 0.0 0.0 4.914035500958073E-5 16-17 3.276023667305382E-5 0.0 0.0 3.276023667305382E-5 6.552047334610764E-5 18-19 3.276023667305382E-5 0.0 0.0 3.276023667305382E-5 6.552047334610764E-5 20-21 3.276023667305382E-5 0.0 0.0 6.552047334610764E-5 9.828071001916147E-5 22-23 3.276023667305382E-5 0.0 0.0 2.948421300574844E-4 9.828071001916147E-5 24-25 3.276023667305382E-5 0.0 0.0 5.896842601149688E-4 9.828071001916147E-5 26-27 3.276023667305382E-5 0.0 0.0 0.0011957486385664644 9.828071001916147E-5 28-29 3.276023667305382E-5 0.0 0.0 0.003849327809083824 9.828071001916147E-5 30-31 3.276023667305382E-5 0.0 0.0 0.012596311000789194 9.828071001916147E-5 32-33 3.276023667305382E-5 0.0 0.0 0.0328093770280634 1.3104094669221528E-4 34-35 4.914035500958073E-5 0.0 0.0 0.06368590009241663 1.9656142003832293E-4 36-37 9.828071001916147E-5 0.0 0.0 0.11441512658064046 1.9656142003832293E-4 38-39 9.828071001916147E-5 0.0 0.0 0.19467770642962234 1.9656142003832293E-4 40-41 9.828071001916147E-5 0.0 0.0 0.2787240936143419 1.9656142003832293E-4 42-43 1.3104094669221528E-4 0.0 0.0 0.36537491961456925 1.9656142003832293E-4 44-45 1.638011833652691E-4 0.0 0.0 0.47641574181788515 1.9656142003832293E-4 46-47 1.638011833652691E-4 0.0 0.0 0.6044755069728526 1.9656142003832293E-4 48-49 1.638011833652691E-4 0.0 0.0 0.7415443372129098 1.9656142003832293E-4 50-51 1.638011833652691E-4 0.0 0.0 0.8923233265006401 2.2932165671137673E-4 52-53 1.638011833652691E-4 1.638011833652691E-5 0.0 1.037598596027297 2.2932165671137673E-4 54-55 1.638011833652691E-4 3.276023667305382E-5 0.0 1.1966167848383003 2.2932165671137673E-4 56-57 1.638011833652691E-4 3.276023667305382E-5 0.0 1.3834156543480534 2.2932165671137673E-4 58-59 1.638011833652691E-4 3.276023667305382E-5 0.0 1.5870696656260923 2.2932165671137673E-4 60-61 1.638011833652691E-4 3.276023667305382E-5 0.0 1.8063339296788414 2.2932165671137673E-4 62-63 1.638011833652691E-4 3.276023667305382E-5 0.0 2.0251886907731778 2.6208189338443056E-4 64-65 1.638011833652691E-4 3.276023667305382E-5 0.0 2.2551327919813424 2.6208189338443056E-4 66-67 1.638011833652691E-4 6.552047334610764E-5 0.0 2.519884644654627 2.784620117209575E-4 68-69 1.638011833652691E-4 6.552047334610764E-5 0.0 2.8167742895041767 2.948421300574844E-4 70-71 1.638011833652691E-4 6.552047334610764E-5 0.0 3.1324519300857236 2.948421300574844E-4 72-73 1.8018130170179602E-4 6.552047334610764E-5 0.0 3.452290120724748 2.948421300574844E-4 74-75 1.9656142003832293E-4 6.552047334610764E-5 0.0 3.784446160352841 2.948421300574844E-4 76-77 1.9656142003832293E-4 6.552047334610764E-5 0.0 4.1416637810358194 2.948421300574844E-4 78-79 1.9656142003832293E-4 6.552047334610764E-5 0.0 4.543009440517402 2.948421300574844E-4 80-81 2.2932165671137673E-4 6.552047334610764E-5 1.638011833652691E-5 4.963912961292797 2.948421300574844E-4 82-83 2.2932165671137673E-4 6.552047334610764E-5 3.276023667305382E-5 5.402359588806613 2.948421300574844E-4 84-85 2.2932165671137673E-4 6.552047334610764E-5 3.276023667305382E-5 5.840052730876948 3.276023667305382E-4 86-87 2.2932165671137673E-4 6.552047334610764E-5 3.276023667305382E-5 6.295747622999127 3.276023667305382E-4 88-89 2.4570177504790365E-4 6.552047334610764E-5 3.276023667305382E-5 6.781123289547093 3.276023667305382E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5330 0.0 48.048264 1 GTATCAA 9020 0.0 39.085304 1 ATCAACG 11765 0.0 29.835508 3 TATCAAC 11885 0.0 29.656593 2 TCAACGC 11900 0.0 29.496073 4 CAACGCA 12085 0.0 29.044538 5 AACGCAG 12490 0.0 28.140772 6 GTACATG 11790 0.0 24.623146 1 TACATGG 11570 0.0 24.14129 2 ACGCAGA 14540 0.0 24.042526 7 CGCAGAG 14555 0.0 23.919851 8 ACATGGG 11520 0.0 23.50192 3 GCAGAGT 16265 0.0 20.704222 9 ACCGTAT 185 6.5614677E-6 20.53927 8 GTACCGT 255 1.1947122E-8 20.48893 6 GAGTACT 8045 0.0 19.984781 12-13 AGAGTAC 14360 0.0 19.812492 10-11 AGTACTT 8665 0.0 19.651121 12-13 TAAGGTG 1540 0.0 19.122192 5 CATGGGG 6435 0.0 18.895489 4 >>END_MODULE