##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938451_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590526 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.184713289507997 31.0 31.0 34.0 28.0 34.0 2 31.104894619373237 31.0 31.0 34.0 28.0 34.0 3 31.094993277180006 31.0 31.0 34.0 27.0 34.0 4 34.786508976742766 37.0 35.0 37.0 32.0 37.0 5 34.819530384775604 37.0 35.0 37.0 32.0 37.0 6 34.91936002817827 37.0 35.0 37.0 32.0 37.0 7 34.85107852998852 37.0 35.0 37.0 32.0 37.0 8 34.86029234953245 37.0 35.0 37.0 32.0 37.0 9 36.276600860927374 39.0 35.0 39.0 32.0 39.0 10-11 36.118396988447586 39.0 35.0 39.0 31.0 39.0 12-13 35.97739980966122 38.0 35.0 39.0 30.0 39.0 14-15 36.718404439431964 39.0 36.0 41.0 30.5 41.0 16-17 36.64051371150466 39.0 36.0 40.0 30.5 41.0 18-19 36.975306760413595 39.0 36.0 41.0 31.0 41.0 20-21 37.038135492764084 39.0 36.0 41.0 31.0 41.0 22-23 36.943149835909004 39.0 36.0 41.0 31.0 41.0 24-25 36.77968624582152 39.0 36.0 41.0 30.5 41.0 26-27 36.479108455851225 39.0 35.0 41.0 30.0 41.0 28-29 36.207647080738184 38.5 35.0 40.5 30.0 41.0 30-31 35.818630339731016 38.0 35.0 40.0 29.0 41.0 32-33 35.36646311932074 38.0 35.0 40.0 28.5 41.0 34-35 35.031341549737014 38.0 34.0 40.0 27.0 41.0 36-37 34.659986181810794 37.0 34.0 40.0 25.0 41.0 38-39 34.305058541029524 37.0 33.0 40.0 24.0 41.0 40-41 34.05855711687546 37.0 33.0 40.0 23.5 41.0 42-43 33.6342599648449 37.0 33.0 40.0 21.5 41.0 44-45 33.0463595167698 37.0 33.0 40.0 17.5 41.0 46-47 32.316594019568996 36.0 31.5 40.0 10.0 41.0 48-49 31.73057325164345 36.0 31.0 40.0 4.5 41.0 50-51 30.941206653051687 35.5 29.5 38.5 2.0 40.5 52-53 30.910323169513283 35.0 30.0 39.0 2.0 40.0 54-55 31.02350362219445 35.0 30.0 39.0 2.0 40.5 56-57 30.75489224860548 35.0 29.5 39.0 2.0 41.0 58-59 30.421632578413146 35.0 29.0 39.0 2.0 40.0 60-61 29.953895340764 35.0 28.0 38.5 2.0 40.0 62-63 29.3826275219042 35.0 27.0 38.0 2.0 40.0 64-65 28.85123771010929 34.5 26.0 38.0 2.0 40.0 66-67 28.36321686090028 34.0 26.0 37.0 2.0 40.0 68-69 27.907987116570652 34.0 25.5 36.5 2.0 39.0 70-71 27.310566342548846 33.5 24.0 36.0 2.0 39.0 72-73 26.68774787223594 33.0 22.5 35.5 2.0 38.5 74-75 26.0573395582921 33.0 20.0 35.0 2.0 37.5 76-77 25.34730138893122 32.0 16.5 35.0 2.0 37.0 78-79 24.612543732198073 31.0 10.0 35.0 2.0 36.0 80-81 23.978205870698325 31.0 4.5 35.0 2.0 36.0 82-83 23.31766425187037 30.5 2.0 34.0 2.0 35.0 84-85 22.643168124688835 30.0 2.0 34.0 2.0 35.0 86-87 21.863381121237676 29.0 2.0 34.0 2.0 35.0 88-89 21.046576272678934 29.0 2.0 34.0 2.0 35.0 90-91 20.140905734887205 27.0 2.0 34.0 2.0 35.0 92-93 19.27161632171996 25.5 2.0 33.0 2.0 35.0 94-95 18.35181346799294 24.5 2.0 33.0 2.0 35.0 96-97 17.17505410430701 20.0 2.0 32.5 2.0 35.0 98-99 15.815014918902808 2.0 2.0 32.0 2.0 34.0 100-101 12.75466448556033 2.0 2.0 28.0 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5040937763789692 1101 2 0.48946115323660067 1101 3 0.46677091290061057 1101 4 0.40778116559634014 1101 5 0.33504307137554434 1101 6 0.42909056295857084 1101 7 0.5335445497839686 1101 8 0.7587441057168149 1101 9 0.7403420335772992 1101 10-11 0.737997961674445 1101 12-13 0.8020965436373544 1101 14-15 0.6960460958578949 1101 16-17 0.6461251333285034 1101 18-19 0.9374178568514182 1101 20-21 0.9495938076437511 1101 22-23 1.0968765685431094 1101 24-25 0.9841660861237642 1101 26-27 1.0676060535849459 1101 28-29 0.9104869673623526 1101 30-31 0.7629478404708223 1101 32-33 0.7964776097323849 1101 34-35 0.5269128108183452 1101 36-37 1.0066770390880535 1101 38-39 1.1675683935711447 1101 40-41 1.1879397755555274 1101 42-43 1.1149034239836908 1101 44-45 1.1355153845053678 1101 46-47 1.0124715472870633 1101 48-49 0.503434572763684 1101 50-51 0.41357533652365674 1101 52-53 0.3408301267058107 1101 54-55 0.43529406484040223 1101 56-57 0.10134908969387268 1101 58-59 0.15371834007034124 1101 60-61 0.443570910903329 1101 62-63 0.4540480871864965 1101 64-65 0.12924518641513671 1101 66-67 0.262725880915891 1101 68-69 0.03285287432758466 1101 70-71 0.2578069240794001 1101 72-73 0.2903040264528123 1101 74-75 0.45949577413987086 1101 76-77 0.2831108808512255 1101 78-79 0.7040262097287808 1101 80-81 0.589136460681221 1101 82-83 0.5153646149467832 1101 84-85 0.6427290185929984 1101 86-87 0.7789701363191277 1101 88-89 0.6724444474831834 1101 90-91 0.38635412565628613 1101 92-93 0.5505755335739977 1101 94-95 0.20864274063357513 1101 96-97 -0.10709350227920922 1101 98-99 0.4733887601594571 1101 100-101 0.4347391477414071 1102 1 0.5489512993083281 1102 2 0.38778783530619165 1102 3 0.3270943743582855 1102 4 0.5666623530219539 1102 5 0.46849892798829984 1102 6 0.5195372478287013 1102 7 0.47144625110722416 1102 8 0.634155433869168 1102 9 0.6704372516687371 1102 10-11 0.790945521710853 1102 12-13 0.8649947442954797 1102 14-15 0.8913954622375115 1102 16-17 1.0546054945971477 1102 18-19 1.0544100853748333 1102 20-21 0.873263554895729 1102 22-23 1.0410147047889993 1102 24-25 1.0544716941892958 1102 26-27 0.8158154633728003 1102 28-29 0.7448109889264458 1102 30-31 1.1350597917415612 1102 32-33 1.0310344255273876 1102 34-35 0.9084555539395467 1102 36-37 0.9571478785475591 1102 38-39 0.8996677424474342 1102 40-41 1.4505474210081601 1102 42-43 1.4596104183126144 1102 44-45 1.673333069865592 1102 46-47 1.6720931253869011 1102 48-49 1.4360852763973817 1102 50-51 1.4768236036944131 1102 52-53 1.3039401176040855 1102 54-55 1.5951904451526566 1102 56-57 1.57254561703407 1102 58-59 1.7359962228680743 1102 60-61 1.849376764015421 1102 62-63 1.9070534081974877 1102 64-65 2.1131386261686913 1102 66-67 1.6936350036492094 1102 68-69 1.99799500133172 1102 70-71 1.9529690048747526 1102 72-73 2.0212464055048294 1102 74-75 2.0111375860841427 1102 76-77 1.698317770160191 1102 78-79 1.9570105267490163 1102 80-81 1.8618645290142055 1102 82-83 2.2493683256518153 1102 84-85 2.1301073704798554 1102 86-87 1.9958188375723616 1102 88-89 1.5064061502067005 1102 90-91 1.2116866888432902 1102 92-93 1.4726402258655327 1102 94-95 1.131853900753999 1102 96-97 1.2109856827659478 1102 98-99 1.4738070896420599 1102 100-101 1.3470947453049433 1103 1 0.7449411740751799 1103 2 0.44999140578314467 1103 3 0.4384053255972944 1103 4 0.5707124462806803 1103 5 0.40923966872904316 1103 6 0.6661266429792434 1103 7 0.7005829417547034 1103 8 0.47841336086090536 1103 9 0.906102053160879 1103 10-11 0.9667382208848423 1103 12-13 1.0445844058995277 1103 14-15 1.2809371622108614 1103 16-17 1.3243696832462248 1103 18-19 1.1715164003228722 1103 20-21 0.9765076263052705 1103 22-23 1.1532915499795067 1103 24-25 0.9757546678103992 1103 26-27 1.157761906218525 1103 28-29 0.9332868748843524 1103 30-31 1.1967104711979957 1103 32-33 1.2411863040245876 1103 34-35 1.0640111094950981 1103 36-37 1.1487412705006577 1103 38-39 1.4186325705849256 1103 40-41 1.100107772726787 1103 42-43 1.2160852384565004 1103 44-45 1.2860029339743022 1103 46-47 1.187713962050907 1103 48-49 1.1081676105300708 1103 50-51 1.2091377523754687 1103 52-53 0.9093624464077052 1103 54-55 1.108166730790817 1103 56-57 0.9058856117049991 1103 58-59 1.2079386689112397 1103 60-61 1.2121532095256775 1103 62-63 1.0929979858686814 1103 64-65 0.9170421700002933 1103 66-67 0.8941279426313606 1103 68-69 1.2323741646677142 1103 70-71 1.3383273848374486 1103 72-73 0.8726519477377117 1103 74-75 1.1474352146479596 1103 76-77 1.0485975463392485 1103 78-79 0.8748096208071061 1103 80-81 1.1035045503304843 1103 82-83 1.0591471692436087 1103 84-85 1.5989056651774298 1103 86-87 1.7935473214519284 1103 88-89 1.5065127316082467 1103 90-91 1.4857407789045745 1103 92-93 1.5429239988471473 1103 94-95 1.5178184624379831 1103 96-97 1.816401350231974 1103 98-99 1.6743533193249807 1103 100-101 1.7028301569756064 1104 1 0.13614054334688674 1104 2 0.10158793677752698 1104 3 0.21655403950288488 1104 4 0.2741839156805028 1104 5 0.28937665503041643 1104 6 0.45684064711839767 1104 7 0.3457613196003706 1104 8 0.2615019584759679 1104 9 0.6346178706438792 1104 10-11 0.48621442074687593 1104 12-13 0.6545368548992343 1104 14-15 0.7396133719280158 1104 16-17 0.9841336528924245 1104 18-19 0.5973134339288677 1104 20-21 0.4169025706237903 1104 22-23 0.6956316505018307 1104 24-25 0.39910639738564413 1104 26-27 0.6654920234945649 1104 28-29 0.660786382127867 1104 30-31 0.5663763823047319 1104 32-33 0.4279902854969393 1104 34-35 0.39106590401564745 1104 36-37 0.5345387968631314 1104 38-39 0.6584386709101437 1104 40-41 0.802735396655045 1104 42-43 0.7359802813263201 1104 44-45 0.6177204366844649 1104 46-47 0.5672288432965971 1104 48-49 0.6398333738098536 1104 50-51 0.6957162977583451 1104 52-53 0.6882564544889078 1104 54-55 1.1086163710785861 1104 56-57 1.5067097469173767 1104 58-59 1.2515784146488933 1104 60-61 1.268358245496902 1104 62-63 1.4174288521345737 1104 64-65 1.1977593770565633 1104 66-67 1.3842235373884328 1104 68-69 1.4307702423261368 1104 70-71 1.5787617496743458 1104 72-73 1.4453458474124936 1104 74-75 1.0150315485397492 1104 76-77 0.9698550608755632 1104 78-79 1.1108030178702748 1104 80-81 1.0786326670152206 1104 82-83 1.052710537726906 1104 84-85 1.283861809850734 1104 86-87 1.7301542003323824 1104 88-89 1.5529180730884882 1104 90-91 1.1969670034044277 1104 92-93 1.3623472273876018 1104 94-95 1.4914683096834729 1104 96-97 1.0843684833747744 1104 98-99 1.5823433656440908 1104 100-101 1.1279927666139251 1105 1 0.4085859339429625 1105 2 0.15915920667756467 1105 3 0.29819047045438296 1105 4 0.22767336403296667 1105 5 0.15417210116147828 1105 6 0.28243764822909867 1105 7 0.2582705754315455 1105 8 0.24069947791321056 1105 9 0.6587755900070746 1105 10-11 0.6100458720611996 1105 12-13 0.3920530526037851 1105 14-15 0.5656384552304985 1105 16-17 0.3588096988013447 1105 18-19 0.3088207460354866 1105 20-21 0.5294071985393742 1105 22-23 0.4527702102944957 1105 24-25 0.513311784279388 1105 26-27 0.4982854333427724 1105 28-29 0.2918142421797043 1105 30-31 0.39735333528511063 1105 32-33 0.41235950060246296 1105 34-35 0.5243151635491543 1105 36-37 0.42413987053954827 1105 38-39 0.43200633103602115 1105 40-41 0.6063845080952532 1105 42-43 0.5756577156099141 1105 44-45 0.8124284152109311 1105 46-47 0.5966739562177352 1105 48-49 0.755473414535512 1105 50-51 0.644334864955681 1105 52-53 0.6582444219083854 1105 54-55 0.36283934405155804 1105 56-57 0.3469888102272627 1105 58-59 0.4493408174626694 1105 60-61 0.7886285157671722 1105 62-63 0.9716680228121 1105 64-65 0.8832462337762976 1105 66-67 0.6024876062518132 1105 68-69 0.7482452515819666 1105 70-71 0.7858956815514304 1105 72-73 0.4640079170163425 1105 74-75 0.4544058543524123 1105 76-77 0.389121073463496 1105 78-79 0.9088060722945634 1105 80-81 1.2284498643495425 1105 82-83 1.0471223296558207 1105 84-85 0.7814900031124914 1105 86-87 1.3950931118466379 1105 88-89 1.2643328268833756 1105 90-91 1.150606859013461 1105 92-93 1.0231094450847493 1105 94-95 1.0356764733265713 1105 96-97 0.8437747218049871 1105 98-99 1.2688020846370573 1105 100-101 1.0281570576172552 1106 1 0.30221890138139074 1106 2 0.2270496226908456 1106 3 0.35363969100599135 1106 4 0.12378534632242122 1106 5 0.2667482011522537 1106 6 0.12439756890062625 1106 7 0.25796502243827746 1106 8 0.10053136611540481 1106 9 0.07672596358884931 1106 10-11 0.204989258280186 1106 12-13 0.22134981950721055 1106 14-15 0.13637397317164357 1106 16-17 0.2071747020298318 1106 18-19 0.1916872943243959 1106 20-21 0.2556836029301195 1106 22-23 0.31283558710476456 1106 24-25 0.0143408637001059 1106 26-27 0.09489206557226737 1106 28-29 0.3146125310829433 1106 30-31 0.1885770461973877 1106 32-33 0.11367568453383825 1106 34-35 -0.09938479494175567 1106 36-37 0.7334056209315776 1106 38-39 0.5091008103276025 1106 40-41 0.7757559913532504 1106 42-43 0.3224090531249004 1106 44-45 0.3302917350153791 1106 46-47 0.30888051004118466 1106 48-49 0.8900291520136072 1106 50-51 0.7932342976648386 1106 52-53 0.9530742346562882 1106 54-55 0.8801607396792654 1106 56-57 -0.017417725147705454 1106 58-59 -0.0913928556302217 1106 60-61 0.7527865731419539 1106 62-63 0.8240455710087637 1106 64-65 0.723914760664961 1106 66-67 0.37556838754505506 1106 68-69 0.29892092725764385 1106 70-71 -0.15476073852052252 1106 72-73 0.1495045271076627 1106 74-75 0.579498096765473 1106 76-77 0.3500621766827585 1106 78-79 0.5928498643801632 1106 80-81 0.9178783072976096 1106 82-83 1.035940819647518 1106 84-85 0.726735483234247 1106 86-87 0.5904971336158482 1106 88-89 0.6419357924769571 1106 90-91 0.9958385027732639 1106 92-93 0.8564475827104303 1106 94-95 0.6862939209790007 1106 96-97 0.8649011169714846 1106 98-99 0.6703909602020399 1106 100-101 0.3926673657069166 1107 1 0.2849043255052557 1107 2 0.2878781695428678 1107 3 0.1363084664992087 1107 4 -0.03324731274137349 1107 5 0.0324104004363619 1107 6 0.17947401386302175 1107 7 0.07591779582266867 1107 8 0.17028912853944433 1107 9 0.3633460140896929 1107 10-11 0.23968084239739795 1107 12-13 0.23602003788175097 1107 14-15 0.32908742249044565 1107 16-17 0.25792527779497476 1107 18-19 0.27039924526643233 1107 20-21 0.2899076380032284 1107 22-23 0.3680923922325334 1107 24-25 0.013211531587664638 1107 26-27 0.1471840270584437 1107 28-29 0.31477485523177506 1107 30-31 0.3886505776494218 1107 32-33 0.4732430976141089 1107 34-35 0.34226314201542607 1107 36-37 0.44967272046263673 1107 38-39 0.4321203169731831 1107 40-41 0.15468021233607487 1107 42-43 0.3852201744101791 1107 44-45 0.2352571891067825 1107 46-47 0.15693955718455044 1107 48-49 0.11426955824019558 1107 50-51 0.11605124597083716 1107 52-53 0.31197986466821703 1107 54-55 0.24388059872086032 1107 56-57 0.27679132615782365 1107 58-59 0.18579297083555346 1107 60-61 0.07943548126926103 1107 62-63 0.24311935219025926 1107 64-65 0.38364449789407473 1107 66-67 0.6202158527910377 1107 68-69 0.8180266183145548 1107 70-71 1.2509816516678853 1107 72-73 1.0313186728941695 1107 74-75 1.038689528270229 1107 76-77 1.0953367403498966 1107 78-79 1.3871144111211962 1107 80-81 1.5304507982102322 1107 82-83 0.888776636401591 1107 84-85 1.034872501464502 1107 86-87 0.9557556036066899 1107 88-89 0.6875488783006496 1107 90-91 0.8907065623753603 1107 92-93 1.13700336949697 1107 94-95 1.4818764843131653 1107 96-97 1.0601049239583595 1107 98-99 0.5579308475463076 1107 100-101 1.036999894356434 1108 1 0.3330994474564761 1108 2 0.2250619093802655 1108 3 0.404249929913842 1108 4 0.29860309376269356 1108 5 0.27123966872904504 1108 6 0.23021872931018095 1108 7 0.42224625110722513 1108 8 0.3691183968321283 1108 9 0.5538891035205893 1108 10-11 0.4172418180071489 1108 12-13 0.5207354850362265 1108 14-15 0.8196695363115793 1108 16-17 0.6639610501527002 1108 18-19 0.6660545298192773 1108 20-21 0.6209450363772149 1108 22-23 0.4683850751593681 1108 24-25 0.5012050275226301 1108 26-27 0.6165043522616926 1108 28-29 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-2.6146319014823227 2316 88-89 -2.83795539646896 2316 90-91 -2.7830008287197963 2316 92-93 -3.3465218041462705 2316 94-95 -3.401107095143267 2316 96-97 -3.540510692212978 2316 98-99 -3.1140209888157617 2316 100-101 -2.7662040238739465 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2556.0 3 691.0 4 641.0 5 710.0 6 845.0 7 1119.0 8 1427.0 9 1750.0 10 2536.0 11 3474.0 12 4937.0 13 6358.0 14 7664.0 15 9510.0 16 11576.0 17 14140.0 18 11560.0 19 8252.0 20 8348.0 21 8956.0 22 9855.0 23 10859.0 24 12213.0 25 13311.0 26 15000.0 27 17110.0 28 19294.0 29 21992.0 30 24782.0 31 28416.0 32 32910.0 33 38894.0 34 47974.0 35 59745.0 36 56082.0 37 51092.0 38 22900.0 39 1047.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.32242025186119 18.249436055845713 14.158046619383422 18.27009707290968 2 15.253564962672145 22.843562046963257 39.94622890349003 21.95664408687456 3 20.536595290168354 30.502687214960243 29.441534001322417 19.51918349354899 4 11.800140722090825 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31.205623913487113 20.561375223653357 50-51 20.26836044570934 29.28526126022377 30.63883368375772 19.807544610309165 52-53 19.438653418530567 29.631414137479613 30.889134642904924 20.040797801084892 54-55 18.45237742406139 29.44108877731872 31.04890166234659 21.0576321362733 56-57 19.469216145381257 30.100644003065476 29.704080379712 20.726059471841275 58-59 19.26071900699766 28.944981593346338 30.309453660709522 21.484845738946483 60-61 19.483470461946283 28.55369188619243 30.91173942317057 21.05109822869072 62-63 20.739367683719923 28.236437784522 30.616684505744633 20.40751002601344 64-65 19.968737324941298 28.586380075209934 31.22330034607409 20.22158225377468 66-67 19.300234813144456 28.721805275293644 31.231926458521563 20.746033453040337 68-69 19.36977789294252 29.30188997194553 30.838831179498644 20.48950095561331 70-71 19.06738568673503 29.40853626000588 30.039612731974174 21.484465321284922 72-73 19.362097849267474 28.81713945968287 29.7441209302168 22.076641760832853 74-75 19.234319921538205 29.098705685261816 30.152231469075176 21.5147429241248 76-77 19.497114207921 28.2238510821191 30.809080399903454 21.469954310056448 78-79 19.115225135287776 28.249510372980208 31.05729800398137 21.577966487750647 80-81 18.669495917005648 29.51517193961707 30.451415584811464 21.36391655856582 82-83 18.691066493253132 28.870729014252426 29.694612664607376 22.743591827887062 84-85 18.951461308929073 27.98273660537377 29.900948013371764 23.164854072325394 86-87 19.458280337300344 27.799841816368108 30.164382262432405 22.577495583899143 88-89 19.843045908859903 27.02642724038624 30.624682364899204 22.505844485854652 90-91 19.13243374416511 27.438203712816982 30.700830839572323 22.728531703445583 92-93 18.76199494200927 27.93447310338052 30.484388154206044 22.819143800404166 94-95 18.77296746899974 27.8944728981748 30.088506820141742 23.24405281268372 96-97 18.98554358525185 27.206284720045677 29.617522536793295 24.190649157909185 98-99 19.578694364128804 26.648983110849134 29.54822241306009 24.224100111961977 100-101 20.310556374667414 26.228498313759385 30.181419153659732 23.27952615791347 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3286.0 1 2204.0 2 1646.0 3 2159.0 4 1729.0 5 1647.0 6 3147.0 7 6189.5 8 8810.5 9 8688.5 10 6280.0 11 4312.5 12 3842.0 13 3689.5 14 3367.5 15 3043.5 16 2835.5 17 2694.5 18 2646.5 19 2662.5 20 2696.5 21 2792.0 22 2927.0 23 3166.5 24 3442.0 25 3740.5 26 4094.5 27 4549.0 28 5081.5 29 5625.5 30 6173.0 31 6556.5 32 7063.0 33 7796.5 34 8644.5 35 9291.5 36 9916.0 37 10774.0 38 11466.5 39 11886.5 40 12397.5 41 13307.0 42 14161.5 43 15204.0 44 16123.5 45 16656.0 46 18083.0 47 21105.5 48 30163.5 49 32296.0 50 25386.5 51 21477.5 52 18091.0 53 16253.5 54 15170.0 55 13851.0 56 12661.5 57 11588.5 58 10748.5 59 9977.0 60 9007.5 61 8172.5 62 6982.5 63 5108.0 64 3596.5 65 2767.0 66 2138.0 67 1651.5 68 1256.0 69 922.5 70 703.0 71 555.5 72 449.5 73 403.5 74 342.5 75 266.5 76 198.5 77 133.5 78 91.5 79 59.5 80 34.0 81 21.0 82 13.0 83 7.0 84 5.5 85 4.5 86 2.5 87 1.0 88 2.0 89 3.0 90 1.5 91 1.0 92 0.5 93 0.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.007112303268611373 2 0.10465246238099593 3 0.11786102559413134 4 0.12040113390434969 5 0.16172022908390146 6 0.100418948530632 7 0.027263829196343597 8 0.02489306144013981 9 0.0233689964540088 10-11 0.05486633950071631 12-13 0.054781669223709036 14-15 0.027517840027365435 16-17 0.032767397201816685 18-19 0.03827096520728977 20-21 0.061809302215313125 22-23 0.03378344052590403 24-25 0.04843139844816316 26-27 0.06003122639816028 28-29 0.05740644781093466 30-31 0.07213907601020107 32-33 0.05156419869743246 34-35 0.0654501241266261 36-37 0.05596705310181093 38-39 0.032682726924809405 40-41 0.037847613822253386 42-43 0.03234404581678029 44-45 0.0066889518835749825 46-47 0.011769168504011678 48-49 0.008043676315691434 50-51 0.00872103853174966 52-53 0.008975049362771496 54-55 0.011091806287953452 56-57 0.013293233490142687 58-59 0.01938949343466672 60-61 0.02430036950108886 62-63 0.010499114348902503 64-65 0.0077049952076623214 66-67 0.009567741301822443 68-69 0.011430487395982564 70-71 0.03141267276970024 72-73 0.02861855362846005 74-75 0.02963459695254739 76-77 0.022606963960943294 78-79 0.004318184127371191 80-81 0.004741535512407582 82-83 0.005080216620436695 84-85 0.004741535512407582 86-87 0.012361860443062624 88-89 0.03827096520728977 90-91 0.03717025160619515 92-93 0.029295915844518277 94-95 0.048939420110206834 96-97 0.0455526090299157 98-99 0.02489306144013981 100-101 0.07721929263063777 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 590526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 46.34798328631878 #Duplication Level Percentage of deduplicated Percentage of total 1 74.76444890596048 34.65181428304291 2 12.645689502547317 11.722044114160799 3 4.972812319565327 6.914394668196418 4 2.521702638112632 4.67503326897241 5 1.3967087022151392 3.2367315793061637 6 0.8731386184412498 2.428092845649271 7 0.5908652177027301 1.9169787864187275 8 0.41164252116535316 1.526304055272793 9 0.2897247430549609 1.2085341793869895 >10 1.3808519390100038 11.026607327947362 >50 0.0842678493176145 2.715951477426307 >100 0.055690056569273706 5.056451590048222 >500 0.005129347315590999 1.672804668487928 >1k 0.006228493168931927 6.387936861149007 >5k 7.327639022272855E-4 2.7303853684516692 >10k+ 3.6638195111364275E-4 2.129934926083046 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12543 2.124038569004582 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8890 1.5054375251894074 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7189 1.2173892428106468 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4826 0.8172375136742497 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4725 0.8001341177187796 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4001 0.6775315566122406 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2707 0.4584048797174045 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2614 0.44265620819405077 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1988 0.3366490213809384 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1883 0.3188682632094099 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 1849 0.31311068437291495 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1792 0.30345827279408527 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1660 0.28110531966416386 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1627 0.27551708138168346 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1608 0.2722996108554069 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1425 0.24131028947074304 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1319 0.22336019074520003 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1312 0.22217480686709817 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1206 0.20422470814155513 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1076 0.1822104361196628 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 946 0.16019616409777046 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 932 0.15782539634156667 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 786 0.1331016754554414 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 778 0.13174695102332495 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 773 0.1309002482532522 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 727 0.12311058276858258 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 697 0.1180303661481459 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 686 0.11616762005398576 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 686 0.11616762005398576 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 666 0.11278080897369464 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.693405540145565E-4 0.0 14-15 0.0 0.0 0.0 1.693405540145565E-4 0.0 16-17 0.0 0.0 0.0 2.540108310218348E-4 0.0 18-19 0.0 0.0 0.0 3.38681108029113E-4 0.0 20-21 0.0 0.0 0.0 6.77362216058226E-4 0.0 22-23 0.0 0.0 0.0 0.002624778587225626 0.0 24-25 0.0 0.0 0.0 0.0073663140996332085 0.0 26-27 0.0 0.0 0.0 0.01312389293612813 0.0 28-29 0.0 0.0 0.0 0.030396629445612895 0.0 30-31 0.0 0.0 0.0 0.07730396290764505 0.0 32-33 0.0 0.0 0.0 0.1498663903028825 0.0 34-35 0.0 0.0 0.0 0.2570589609940968 0.0 36-37 0.0 0.0 0.0 0.4177631467539109 0.0 38-39 0.0 0.0 0.0 0.6375671858648053 0.0 40-41 0.0 0.0 0.0 0.8837036811249632 0.0 42-43 0.0 0.0 0.0 1.0989355252774646 0.0 44-45 0.0 0.0 0.0 1.3371130144989383 0.0 46-47 0.0 0.0 0.0 1.6364224437196668 0.0 48-49 0.0 0.0 0.0 1.9658744915549864 0.0 50-51 0.0 0.0 0.0 2.3179335033512496 0.0 52-53 0.0 0.0 0.0 2.6335842960343827 0.0 54-55 0.0 0.0 0.0 2.951013164534669 0.0 56-57 0.0 0.0 0.0 3.3146720042809292 0.0 58-59 0.0 0.0 0.0 3.7295563616165928 0.0 60-61 0.0 0.0 0.0 4.243843624158801 0.0 62-63 0.0 0.0 0.0 4.716811791521457 0.0 64-65 0.0 0.0 0.0 5.126107910574641 0.0 66-67 0.0 0.0 0.0 5.579263233117594 0.0 68-69 0.0 0.0 0.0 6.068488093665647 0.0 70-71 0.0 0.0 0.0 6.648310150611488 0.0 72-73 0.0 0.0 0.0 7.2228826503828785 0.0 74-75 0.0 0.0 0.0 7.780182413644784 0.0 76-77 0.0 0.0 0.0 8.335280749704502 0.0 78-79 0.0 0.0 0.0 8.930343456511652 0.0 80-81 0.0 0.0 0.0 9.584167335561855 0.0 82-83 0.0 0.0 0.0 10.251369118379207 0.0 84-85 0.0 0.0 0.0 10.964800872442535 0.0 86-87 0.0 0.0 0.0 11.70100893102082 0.0 88-89 0.0 0.0 0.0 12.36922675716226 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CATATAG 75 3.0580504E-4 31.699408 3 ATGGGCG 105 6.851851E-5 27.170923 5 GTATAGG 70 0.008349589 27.168617 1 GTACATG 9495 0.0 26.589193 1 TACATGG 9480 0.0 26.432892 2 GTCGGGA 370 0.0 25.702223 2 ATGGGAG 1725 0.0 25.635174 5 CATGGGT 520 0.0 25.603369 4 ATGGGAT 1960 0.0 25.472736 5 CATGGGG 1905 0.0 25.459368 4 CATAAGG 95 0.0012184832 25.02585 2 ACATGGG 9775 0.0 24.710945 3 CGGGAGT 350 0.0 24.453829 4 GAGTACT 7055 0.0 23.67525 12-13 ATGGGGG 925 0.0 23.132002 5 AGTACTT 7100 0.0 22.956305 12-13 TCGGGAG 415 0.0 22.915236 3 GGGGCTA 105 0.0021818182 22.642435 5 CATGGGA 7495 0.0 22.394712 4 CATGGGC 725 0.0 22.298895 4 >>END_MODULE