##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938451_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 590526 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.706632730819642 31.0 31.0 34.0 30.0 34.0 2 31.851766052637817 31.0 31.0 34.0 30.0 34.0 3 31.865911069114652 31.0 31.0 34.0 30.0 34.0 4 35.5937994262742 37.0 35.0 37.0 33.0 37.0 5 35.52432238377311 37.0 35.0 37.0 33.0 37.0 6 35.58070262782672 37.0 35.0 37.0 33.0 37.0 7 35.501077005923534 37.0 35.0 37.0 33.0 37.0 8 35.51881373555102 37.0 35.0 37.0 33.0 37.0 9 37.009796351049744 39.0 37.0 39.0 33.0 39.0 10-11 36.9273291946502 39.0 37.0 39.0 33.0 39.0 12-13 36.794116601131876 39.0 37.0 39.0 32.0 39.0 14-15 37.556989870048056 39.0 36.0 41.0 32.0 41.0 16-17 37.55320764877415 39.0 36.5 41.0 32.0 41.0 18-19 37.95485973521911 40.0 37.0 41.0 33.0 41.0 20-21 37.99919478566566 40.0 37.0 41.0 33.0 41.0 22-23 37.871658487517905 40.0 37.0 41.0 32.5 41.0 24-25 37.70727876503321 40.0 36.5 41.0 32.0 41.0 26-27 37.40204664993582 39.0 36.0 41.0 32.0 41.0 28-29 37.19718268120286 39.0 36.0 41.0 31.5 41.0 30-31 36.76928026877733 38.5 35.0 40.0 31.0 41.0 32-33 36.355757409495936 38.0 35.0 40.0 30.5 41.0 34-35 36.06320890189424 38.0 35.0 40.0 30.0 41.0 36-37 35.86302719947979 38.0 35.0 40.0 30.0 41.0 38-39 35.58102014136549 38.0 35.0 40.0 29.5 41.0 40-41 35.25324710512323 38.0 34.5 40.0 27.5 41.0 42-43 34.904652801062106 38.0 34.0 40.0 26.5 41.0 44-45 34.35261021529958 38.0 33.5 40.0 23.5 41.0 46-47 33.579961762902904 37.0 33.0 40.0 21.0 41.0 48-49 32.96651883236301 37.0 33.0 40.0 14.0 41.0 50-51 32.535475999363285 37.0 32.5 40.0 8.5 41.0 52-53 31.99806443746761 36.0 31.0 40.0 6.0 41.0 54-55 31.684107050324627 35.5 31.0 39.0 2.0 41.0 56-57 31.406082882040756 35.0 31.0 39.0 2.0 40.5 58-59 30.97439062801638 35.0 30.0 39.0 2.0 40.0 60-61 30.559369104831966 35.0 29.0 38.5 2.0 40.0 62-63 30.047295970033495 35.0 28.0 38.0 2.0 40.0 64-65 29.49348631558983 34.5 28.0 38.0 2.0 40.0 66-67 29.008783694536735 34.0 26.5 37.0 2.0 39.5 68-69 28.611964587503344 34.0 26.0 36.5 2.0 39.0 70-71 28.13704053674182 33.5 26.0 36.0 2.0 39.0 72-73 27.69671699467932 33.0 26.0 35.5 2.0 38.5 74-75 27.151432790427517 33.0 24.5 35.0 2.0 37.5 76-77 25.916405882213486 31.5 23.0 34.0 2.0 36.0 78-79 26.562457876537188 32.5 24.0 35.0 2.0 36.0 80-81 26.254216579794964 33.0 23.5 35.0 2.0 36.0 82-83 25.858139184388158 32.0 22.0 35.0 2.0 36.0 84-85 25.36855447516282 32.0 20.0 35.0 2.0 35.0 86-87 24.785014546353587 31.5 17.5 34.5 2.0 35.0 88-89 24.046257912437387 31.0 9.0 34.0 2.0 35.0 90-91 23.452827648570935 31.0 3.5 34.0 2.0 35.0 92-93 22.719112282947748 30.0 2.0 34.0 2.0 35.0 94-95 22.017387041383444 29.5 2.0 34.0 2.0 35.0 96-97 21.058183720953863 29.0 2.0 34.0 2.0 35.0 98-99 19.87902056810369 28.5 2.0 34.0 2.0 35.0 100-101 16.465186968905687 20.0 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3492240497045067 1101 2 0.38266990740812545 1101 3 0.318628208100602 1101 4 0.25677005837744105 1101 5 0.13007544508690927 1101 6 0.20216243920148713 1101 7 0.13593746087049396 1101 8 0.050333455994589826 1101 9 0.2497391833531708 1101 10-11 0.2414528351376788 1101 12-13 0.46755373599501837 1101 14-15 0.2901047550605256 1101 16-17 0.45448472995943234 1101 18-19 0.3675655543818763 1101 20-21 0.41412518986279423 1101 22-23 0.3984471235608211 1101 24-25 0.46945639154736085 1101 26-27 0.4902121659296199 1101 28-29 0.43981077469647545 1101 30-31 0.4965552961014552 1101 32-33 0.2426021205360982 1101 34-35 0.4821753945147762 1101 36-37 0.5073918263096786 1101 38-39 0.4310902061620041 1101 40-41 0.6136905703235627 1101 42-43 0.8040495719651588 1101 44-45 0.3443104570990485 1101 46-47 0.6655178233995045 1101 48-49 0.4199670330357179 1101 50-51 0.21032545086422516 1101 52-53 0.04242309065477201 1101 54-55 0.015452573742582132 1101 56-57 -0.09247490257801871 1101 58-59 0.33532462855939116 1101 60-61 0.5272686686037993 1101 62-63 0.3743393728438953 1101 64-65 0.2307929464839482 1101 66-67 0.023135943834688533 1101 68-69 0.05520850846550118 1101 70-71 0.03196824670649079 1101 72-73 0.3055837574110001 1101 74-75 0.10766433510821471 1101 76-77 0.244426068333933 1101 78-79 0.08283799962555705 1101 80-81 0.05575646636413367 1101 82-83 0.2900860839630859 1101 84-85 0.6234012941231555 1101 86-87 0.5395798637517686 1101 88-89 0.6382981385851316 1101 90-91 0.5279825434408849 1101 92-93 0.47997674364906473 1101 94-95 0.06657114472484693 1101 96-97 0.27508236061219193 1101 98-99 -0.033140706862194946 1101 100-101 0.4076686797178617 1102 1 0.279088223897606 1102 2 0.2719543714562924 1102 3 0.2073315617377922 1102 4 0.0041795661839287845 1102 5 0.03843503340868892 1102 6 0.1655034739981005 1102 7 0.22481234709888298 1102 8 0.24032505439829066 1102 9 0.3757141185908708 1102 10-11 0.45640942689010444 1102 12-13 0.33347826165489636 1102 14-15 0.3891928317950999 1102 16-17 0.2308937476727948 1102 18-19 0.4412283003035995 1102 20-21 0.29597074051741856 1102 22-23 0.21861620568642337 1102 24-25 0.23489607508722798 1102 26-27 0.4584576325682832 1102 28-29 0.49568139011340406 1102 30-31 0.41224877786298464 1102 32-33 0.3911213391876416 1102 34-35 0.4530883679797384 1102 36-37 0.6178238083862766 1102 38-39 0.610315508969542 1102 40-41 0.5087668848233236 1102 42-43 0.3814492779092902 1102 44-45 0.9006641643034143 1102 46-47 1.0679962943089762 1102 48-49 0.9515202781522873 1102 50-51 1.4194394325207398 1102 52-53 1.3532856545418994 1102 54-55 1.2352908430137433 1102 56-57 1.2711566874240816 1102 58-59 0.9784318189255607 1102 60-61 1.27079068778745 1102 62-63 1.2532146091387908 1102 64-65 1.1134208957943166 1102 66-67 0.8981569478254485 1102 68-69 0.8385873504454757 1102 70-71 1.1964417466714856 1102 72-73 1.3628948965274326 1102 74-75 1.071719505590604 1102 76-77 1.281123540853713 1102 78-79 1.2804330426837431 1102 80-81 1.4364205425386025 1102 82-83 1.2774189272032999 1102 84-85 0.7502531459750088 1102 86-87 0.8669288100515651 1102 88-89 1.1675882036975054 1102 90-91 1.4529807931083205 1102 92-93 1.3989083406524934 1102 94-95 1.4547896562420313 1102 96-97 1.2747865544090153 1102 98-99 1.604295405999249 1102 100-101 1.1435049886165558 1103 1 0.2824188926958975 1103 2 0.31641213857593087 1103 3 0.30755804721608015 1103 4 0.17031332584286218 1103 5 0.05925837473562723 1103 6 0.19750453981211535 1103 7 0.1787425362808719 1103 8 0.24328268829117405 1103 9 0.39668400934493064 1103 10-11 0.36335720200334976 1103 12-13 0.3027694953346156 1103 14-15 0.43165219763575635 1103 16-17 0.3778894844167411 1103 18-19 0.4554502560723179 1103 20-21 0.5395025817111332 1103 22-23 0.5771693631604435 1103 24-25 0.655952563230052 1103 26-27 0.6243715680865307 1103 28-29 0.6772361463084522 1103 30-31 0.8492058788488634 1103 32-33 0.8285913631684565 1103 34-35 0.9655650532981568 1103 36-37 0.8730063290364214 1103 38-39 0.6459536651112998 1103 40-41 0.5818417582579016 1103 42-43 0.5605060325056144 1103 44-45 0.7254643241755261 1103 46-47 0.5440554469868317 1103 48-49 0.6866521726366983 1103 50-51 0.6503813456569034 1103 52-53 0.5360261288291426 1103 54-55 0.33988716595538904 1103 56-57 0.34020145161273163 1103 58-59 0.5365186827678237 1103 60-61 0.7287976155785465 1103 62-63 0.7591498609373524 1103 64-65 0.8524763181231201 1103 66-67 0.7682808487047446 1103 68-69 0.7758042435976229 1103 70-71 0.7430577871724182 1103 72-73 0.848519729993992 1103 74-75 0.7913504674877565 1103 76-77 0.29773691681960557 1103 78-79 0.405832722939536 1103 80-81 0.5702401535164903 1103 82-83 0.8557029666384217 1103 84-85 0.7879696394468958 1103 86-87 0.9372658737339528 1103 88-89 0.9092348863513813 1103 90-91 0.6974518829031524 1103 92-93 0.953737810410022 1103 94-95 0.6684720436654281 1103 96-97 0.7310482117498083 1103 98-99 1.1219146439422296 1103 100-101 1.2706499393226558 1104 1 0.09174677285752608 1104 2 -0.07440534442397251 1104 3 -0.007572040494576271 1104 4 0.010204426660841648 1104 5 -0.06586481438431235 1104 6 0.008704894596775148 1104 7 0.11532477733733515 1104 8 -0.1003903185088646 1104 9 0.029075356541149233 1104 10-11 0.20272474906158777 1104 12-13 0.21497876901156587 1104 14-15 -0.0034948901734424 1104 16-17 0.3839442296616369 1104 18-19 0.268917290663822 1104 20-21 0.07762345487561362 1104 22-23 0.09785897585386039 1104 24-25 0.11190013394060117 1104 26-27 -0.06242009447382202 1104 28-29 0.05171259254871785 1104 30-31 0.035720365889375216 1104 32-33 -0.041641217889981874 1104 34-35 0.19872510060277193 1104 36-37 0.07616982563640562 1104 38-39 -0.1315667842826187 1104 40-41 -0.049484003050856984 1104 42-43 0.20726739054246934 1104 44-45 -0.12460219794924399 1104 46-47 -0.2879207528752019 1104 48-49 -0.25607104604862485 1104 50-51 -0.3588393599703821 1104 52-53 -0.27465067219579 1104 54-55 -0.011836249832526846 1104 56-57 -0.031164469349128865 1104 58-59 -0.42770719680249414 1104 60-61 -0.16792062626633353 1104 62-63 -0.19250456894339862 1104 64-65 -0.11407783581400466 1104 66-67 -0.13601352401625633 1104 68-69 0.05199425049621098 1104 70-71 0.08347244175012491 1104 72-73 -0.11010104461919568 1104 74-75 0.021960500009683415 1104 76-77 -0.16467808268763662 1104 78-79 0.4895274110246852 1104 80-81 -0.21000893488402284 1104 82-83 0.0072071057928511095 1104 84-85 0.48299033521904633 1104 86-87 0.331261636028227 1104 88-89 0.24374878208411133 1104 90-91 0.21033746484855698 1104 92-93 0.23787967500053853 1104 94-95 0.2424101534515728 1104 96-97 0.27371476343996193 1104 98-99 0.17332762314790529 1104 100-101 0.755713258530303 1105 1 -0.03422508941570612 1105 2 0.06517258967451056 1105 3 0.2172289591351877 1105 4 -0.08415877215441014 1105 5 0.01204614451980035 1105 6 -0.026513543018921837 1105 7 -0.016316281529746846 1105 8 -0.12480257322933852 1105 9 0.2260394439161928 1105 10-11 -0.09134457711390098 1105 12-13 0.009591875268505134 1105 14-15 0.10705444340671022 1105 16-17 -0.10103818425913857 1105 18-19 0.23104937137467374 1105 20-21 0.08893870848538654 1105 22-23 0.10063572520594022 1105 24-25 -0.04724231330116169 1105 26-27 0.05082499993565648 1105 28-29 -0.06775704832503493 1105 30-31 0.10664317225737818 1105 32-33 0.39134656441286353 1105 34-35 0.30050453414590805 1105 36-37 0.23372721178967737 1105 38-39 0.4095522457062728 1105 40-41 0.7381525204589536 1105 42-43 0.581712040672052 1105 44-45 0.4864562063454585 1105 46-47 0.6042950931077726 1105 48-49 0.9601914493234602 1105 50-51 1.1684884815697885 1105 52-53 1.088652271158523 1105 54-55 0.8353846868575872 1105 56-57 0.6822865673039615 1105 58-59 0.9420516887954342 1105 60-61 0.8630516987984578 1105 62-63 0.6723024469766301 1105 64-65 0.6503265514499716 1105 66-67 0.7278679087864077 1105 68-69 0.7050162793744086 1105 70-71 0.7411802351599732 1105 72-73 0.5880200216525573 1105 74-75 1.0949427288138303 1105 76-77 0.7959383556685253 1105 78-79 1.002092201842899 1105 80-81 1.205739861857925 1105 82-83 1.405728486762861 1105 84-85 1.402117510339373 1105 86-87 1.3533715027442597 1105 88-89 1.309742858352159 1105 90-91 1.1687903526678802 1105 92-93 1.0821040363481877 1105 94-95 1.1615026192049953 1105 96-97 1.214338606461066 1105 98-99 1.0071044650583083 1105 100-101 1.043210944572511 1106 1 0.10941940832435293 1106 2 0.1788498334712081 1106 3 0.24715585702283605 1106 4 0.14976798736961427 1106 5 7.406837689458712E-4 1106 6 0.1414483608644872 1106 7 0.24462020956885766 1106 8 0.3998434465105376 1106 9 0.04767720796009911 1106 10-11 0.38741372470327917 1106 12-13 0.15606706205939247 1106 14-15 0.45360630670345614 1106 16-17 0.5795032787055376 1106 18-19 0.3133209560993251 1106 20-21 0.2240392527156274 1106 22-23 0.40764730162879204 1106 24-25 0.41791023258944193 1106 26-27 0.42863839353402966 1106 28-29 0.40485848529732493 1106 30-31 0.3298698118662742 1106 32-33 0.3241133755926455 1106 34-35 0.4255823636819258 1106 36-37 0.45683278323142673 1106 38-39 0.20731652021969893 1106 40-41 0.2603627639789252 1106 42-43 0.11135068101888379 1106 44-45 0.03335282566101938 1106 46-47 -0.9040932013300065 1106 48-49 -0.6142078219614788 1106 50-51 0.08513593106707162 1106 52-53 0.06326542953874537 1106 54-55 0.25119907206190817 1106 56-57 0.46579074127315323 1106 58-59 -0.23598481613953837 1106 60-61 -0.05322966362198045 1106 62-63 -0.03765569802844482 1106 64-65 0.2694662756451578 1106 66-67 0.27990704263014976 1106 68-69 0.050539063250433 1106 70-71 -0.0733692759552369 1106 72-73 0.454441477237836 1106 74-75 0.5553246124039042 1106 76-77 0.4938905740985575 1106 78-79 0.8408236380572696 1106 80-81 0.21133205814977885 1106 82-83 0.7729599460378331 1106 84-85 0.7411487343961909 1106 86-87 0.9620590085094101 1106 88-89 0.9944179344521515 1106 90-91 1.0605525286664026 1106 92-93 0.6518220051702528 1106 94-95 0.6552099570889567 1106 96-97 0.8792392152609914 1106 98-99 0.7738378153027519 1106 100-101 0.7924874181450612 1107 1 0.07958506219932104 1107 2 0.08220730731898485 1107 3 0.18949054999434267 1107 4 0.08850657612069313 1107 5 0.10970874614582016 1107 6 0.009044576075787347 1107 7 0.0013254455631965811 1107 8 0.003062181769557526 1107 9 -0.2205821777054311 1107 10-11 0.22106615679074082 1107 12-13 0.17620635560249553 1107 14-15 0.19219644516456924 1107 16-17 0.2612008840774962 1107 18-19 0.13244329578688507 1107 20-21 0.198590433381014 1107 22-23 0.2499847693720625 1107 24-25 0.09643627382254749 1107 26-27 0.13844859914461694 1107 28-29 0.24906892398874447 1107 30-31 0.05484137045557702 1107 32-33 0.18217824975675256 1107 34-35 0.2983909876725974 1107 36-37 0.375457051385375 1107 38-39 0.3088024105052227 1107 40-41 0.6014723952117578 1107 42-43 0.3646471098876063 1107 44-45 0.21150427270450223 1107 46-47 0.4633892482385136 1107 48-49 0.4574145870953785 1107 50-51 0.4157583123762052 1107 52-53 0.5026533148851726 1107 54-55 0.28637485385385375 1107 56-57 0.6368748446056571 1107 58-59 0.707519443135137 1107 60-61 0.6064501277151813 1107 62-63 0.6802471294639929 1107 64-65 0.3419890981430065 1107 66-67 0.41936290988506997 1107 68-69 0.36586828992153997 1107 70-71 0.6617759833471837 1107 72-73 0.35858143672616904 1107 74-75 0.36919014696368535 1107 76-77 0.19309282721324195 1107 78-79 0.4298910372636833 1107 80-81 0.38599145491439657 1107 82-83 0.29839905367123265 1107 84-85 0.6396614567247809 1107 86-87 0.645135675453556 1107 88-89 0.5389974177896484 1107 90-91 0.6408308382754413 1107 92-93 0.48029948789729815 1107 94-95 0.7939721314001176 1107 96-97 0.3659563827739625 1107 98-99 0.30492774565597713 1107 100-101 0.824850968743025 1108 1 -0.006971035361654998 1108 2 -0.017838221136301513 1108 3 0.0333019321081629 1108 4 -0.13687228566792697 1108 5 0.046346144519802124 1108 6 0.14814051103513748 1108 7 0.26368642117295593 1108 8 0.1097028321760618 1108 9 0.11541782229457453 1108 10-11 0.23059461207529353 1108 12-13 0.35584863202526407 1108 14-15 0.688755794758066 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2316 72-73 -0.7643925198722741 2316 74-75 -0.6924911390449253 2316 76-77 -0.8007991253633406 2316 78-79 -1.0541226814774483 2316 80-81 -1.2373711171028283 2316 82-83 -0.8203063018193468 2316 84-85 -1.1717428636917155 2316 86-87 -1.2715118688671083 2316 88-89 -1.1383382842471939 2316 90-91 -0.7104121142147832 2316 92-93 -1.0767899676710044 2316 94-95 -1.4760637692623817 2316 96-97 -1.8186223704490772 2316 98-99 -1.5949402484716124 2316 100-101 -1.6067872611445875 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 5.0 4 18.0 5 62.0 6 165.0 7 360.0 8 579.0 9 1038.0 10 1726.0 11 2639.0 12 3507.0 13 4972.0 14 6108.0 15 8191.0 16 10433.0 17 13300.0 18 11466.0 19 7149.0 20 6402.0 21 6514.0 22 7222.0 23 8022.0 24 9143.0 25 10397.0 26 12033.0 27 13788.0 28 16344.0 29 19247.0 30 22411.0 31 26210.0 32 31156.0 33 37776.0 34 49030.0 35 64997.0 36 69635.0 37 73373.0 38 33815.0 39 1291.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.845892539325305 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19.264520105803978 27.35798254437569 35.01928788910226 18.358209460718072 20-21 21.32725739425529 25.781930008162213 34.20848531648056 18.682327281101934 22-23 20.22137890626323 26.434399162780302 33.62620782150151 19.71801410945496 24-25 19.89137871469893 26.58537287550043 34.73569497977957 18.787553430021067 26-27 20.194339827133064 26.20268512748259 35.106991898555826 18.495983146828515 28-29 18.992522748844458 26.469350551924602 35.92662116474422 18.611505534486714 30-31 18.86296245493356 27.874896065650944 34.97697780080845 18.28516367860705 32-33 18.45343480296653 27.95198820026028 34.41839120167513 19.17618579509806 34-35 18.953802276861172 27.235706954892947 34.412206035274075 19.398284732971806 36-37 19.85411313557671 27.027095694533603 34.33270267569348 18.7860884941962 38-39 20.2236754200053 26.465604908395196 34.67121423678468 18.639505434814822 40-41 18.969966893304996 27.486261991651357 34.83755704766179 18.70621406738186 42-43 18.715273955784358 28.23450716747246 33.86408473959171 19.18613413715147 44-45 18.878184800344805 28.5824849423478 32.778474479682224 19.76085577762517 46-47 19.01025526395112 28.463268340428705 31.736621249530078 20.789855146090098 48-49 19.630211032198414 28.23067908948971 31.58286002648486 20.556249851827015 50-51 20.14963032706656 28.933638374184195 30.980302173331392 19.936429125417853 52-53 19.39101749965285 29.318353467925206 31.062391833721126 20.228237198700818 54-55 18.4139714386836 29.108038860730982 31.227191673764942 21.250798026820476 56-57 19.56560761084186 29.72036794315576 29.739503425759406 20.97452102024297 58-59 19.19815447310766 28.719826626163087 30.429005395490012 21.65301350523924 60-61 19.40727419283825 28.555558942366638 30.79119293646681 21.245973928328304 62-63 20.620108827270155 28.321594236524028 30.472990480577565 20.585306455628245 64-65 19.76334557845942 28.58517137248725 31.07501367626461 20.576469372788722 66-67 19.197179306105237 28.814228111086837 30.857944124655162 21.130648458152766 68-69 19.278683749001793 29.374215090750965 30.46476789550746 20.88233326473978 70-71 19.081902474998962 29.431751888783804 29.676025844777236 21.810319791439998 72-73 19.297134501389017 28.87580637923341 29.28222405298332 22.544835066394253 74-75 19.143326809380603 29.03952228066773 29.631251286229322 22.185899623722346 76-77 19.146703036053133 28.30535036595283 30.68531444835999 21.86263214963405 78-79 18.764758294288434 28.31686841276133 30.937365544951607 21.981007747998632 80-81 18.29137225309277 29.43525950317471 30.30513992531783 21.968228318414685 82-83 18.20990033261987 29.0268645389272 29.510421157571358 23.252813970881576 84-85 18.468711839317383 28.030371034041234 29.544265121580676 23.95665200506071 86-87 18.898312547842757 27.86827915105779 29.847021724845717 23.386386576253734 88-89 19.199461053487898 27.24222086638194 30.03372652701512 23.524591553115044 90-91 18.34218349043421 27.46843161665693 30.52491171015524 23.664473182753625 92-93 17.847466943097157 28.123668767843274 30.04860400061994 23.980260288439634 94-95 17.8752095072018 27.935366340121977 29.72186251584802 24.46756163682821 96-97 18.017203044201914 27.40018888620665 29.249579664663454 25.333028404927983 98-99 18.455807854047148 27.13548214388582 29.130677139633153 25.27803286243388 100-101 18.756705718967392 26.942784921606073 29.664280058768984 24.636229300657554 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3363.0 1 2244.5 2 1609.5 3 2112.5 4 1743.0 5 1642.5 6 3074.0 7 6413.0 8 9007.0 9 8658.0 10 6286.5 11 4279.0 12 3741.0 13 3513.5 14 3294.5 15 2976.0 16 2733.5 17 2626.5 18 2535.0 19 2532.5 20 2520.5 21 2662.5 22 2855.0 23 3034.5 24 3338.5 25 3607.5 26 3949.0 27 4356.0 28 4861.5 29 5534.5 30 5998.0 31 6373.0 32 7026.5 33 7646.0 34 8435.0 35 9164.5 36 9865.0 37 10875.0 38 11716.5 39 12079.0 40 12590.5 41 13586.5 42 14532.5 43 15477.0 44 16318.0 45 16791.0 46 17783.5 47 20859.0 48 31720.0 49 32842.0 50 25112.0 51 22535.0 52 18520.0 53 16275.5 54 15057.0 55 13928.0 56 12903.5 57 11872.0 58 10970.5 59 10215.5 60 9192.5 61 7976.5 62 6616.5 63 4781.5 64 3282.5 65 2501.0 66 1895.0 67 1408.5 68 1052.0 69 738.0 70 535.0 71 413.5 72 359.0 73 341.0 74 282.5 75 213.5 76 147.5 77 97.5 78 75.5 79 55.5 80 35.0 81 19.0 82 8.0 83 4.0 84 2.5 85 1.0 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 5.080216620436696E-4 2 0.05503568005473087 3 0.0 4 0.0 5 0.042335138503639125 6 1.693405540145565E-4 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005757578836494921 26-27 0.002370767756203791 28-29 2.540108310218348E-4 30-31 0.0015240649861310086 32-33 7.620324930655043E-4 34-35 0.010837795456931617 36-37 0.023199655899994245 38-39 0.004656865235400305 40-41 0.0018627460941601215 42-43 0.0018627460941601215 44-45 0.0077049952076623214 46-47 0.0 48-49 0.0 50-51 6.77362216058226E-4 52-53 0.0 54-55 0.0011853838781018956 56-57 0.0 58-59 0.021252239528826843 60-61 0.0 62-63 0.00829768714671327 64-65 0.014732628199266417 66-67 0.005588238282480365 68-69 0.015494660692331921 70-71 8.467027700727825E-5 72-73 8.467027700727825E-5 74-75 0.023284326177001522 76-77 0.04843139844816316 78-79 0.030481299722620175 80-81 0.02667113725729265 82-83 0.035984867728093256 84-85 0.01608735263138287 86-87 0.008467027700727825 88-89 0.08229950925107447 90-91 0.02387701811605247 92-93 0.024131028947074303 94-95 0.02582443448721987 96-97 0.037847613822253386 98-99 0.052156890636483405 100-101 0.013293233490142687 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 590526.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.70959621286366 #Duplication Level Percentage of deduplicated Percentage of total 1 72.58788459064166 31.727871254028955 2 13.571671998671636 11.86424605990731 3 5.371135393416209 7.043104777525293 4 2.704378662335429 4.728291973494637 5 1.5502214821914118 3.387977751354681 6 1.0090256721817172 2.6462462819685713 7 0.6468993444063484 1.9792996394057398 8 0.47144021072889314 1.6485168999573818 9 0.3500375074349466 1.3769998298404897 >10 1.5663655170676132 11.799390883741765 >50 0.09496645617730935 2.938361099984196 >100 0.06240652818488956 5.424353848610627 >500 0.005426654624773005 1.6610558824268675 >1k 0.006977127374708149 6.701659060525779 >5k 7.752363749675722E-4 2.9769903009100864 >10k+ 3.876181874837861E-4 2.0956344563176175 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12369 2.0945733126060495 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9496 1.6080579009222287 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8075 1.367424973667544 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4969 0.8414532128983312 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4705 0.7967473066384885 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 3967 0.6717739777757457 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2773 0.46958135628236525 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2706 0.45823553916338994 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2056 0.34816417905392816 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1895 0.3209003498575846 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1892 0.3203923281955409 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1852 0.31361870603495867 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1757 0.29753135340357584 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1637 0.27721048692182904 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1511 0.2558735771159949 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1492 0.2526561065897183 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1414 0.23944754337658294 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1373 0.23250458066198607 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1311 0.22200546631308357 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1126 0.19067746382039064 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1119 0.18949207994228873 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 969 0.16409099684010525 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 847 0.14343144925032936 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 819 0.13868991373792178 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 784 0.13276299434741232 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 741 0.12548135052478637 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 731 0.12378794498464081 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 721 0.12209453944449523 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 676 0.1144742145138402 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 669 0.11328883063573832 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 623 0.1054991651510687 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.693405540145565E-4 0.0 14-15 0.0 0.0 0.0 1.693405540145565E-4 0.0 16-17 0.0 0.0 0.0 2.540108310218348E-4 0.0 18-19 0.0 0.0 0.0 3.38681108029113E-4 0.0 20-21 0.0 0.0 0.0 6.77362216058226E-4 0.0 22-23 0.0 0.0 0.0 0.002624778587225626 0.0 24-25 0.0 0.0 0.0 0.007620324930655044 0.0 26-27 0.0 0.0 0.0 0.013377903767149965 0.0 28-29 0.0 0.0 0.0 0.030396629445612895 0.0 30-31 0.0 0.0 0.0 0.07738863318465232 0.0 32-33 0.0 0.0 0.0 0.15130578501200626 0.0 34-35 0.0 0.0 0.0 0.2597684098583297 0.0 36-37 0.0 0.0 0.0 0.42335138503639125 0.0 38-39 0.0 0.0 0.0 0.6445948188564093 0.0 40-41 0.0 0.0 0.0 0.8942027954738656 0.0 42-43 0.0 0.0 0.0 1.1134988129227164 0.0 44-45 0.0 0.0 0.0 1.353793059069372 0.0 46-47 0.0 0.0 0.0 1.6566586399244065 0.0 48-49 0.0 0.0 0.0 1.988566125792937 0.0 50-51 0.0 0.0 0.0 2.341471840359273 0.0 52-53 0.0 0.0 0.0 2.6575459844274425 0.0 54-55 0.0 0.0 0.0 2.977176280129918 0.0 56-57 0.0 0.0 0.0 3.3424438551393165 0.0 58-59 0.0 0.0 0.0 3.7574128827519875 0.0 60-61 0.0 0.0 0.0 4.273901572496385 0.0 62-63 0.0 0.0 0.0 4.750510561770354 0.0 64-65 0.0 0.0 0.0 5.161415416086675 0.0 66-67 0.0 0.0 0.0 5.619312274142036 0.0 68-69 0.0 0.0 0.0 6.113193999925491 0.0 70-71 0.0 0.0 0.0 6.697842262660746 0.0 72-73 0.0 0.0 0.0 7.277071627667537 0.0 74-75 0.0 0.0 0.0 7.842076386137105 0.0 76-77 0.0 0.0 0.0 8.40682713377565 0.0 78-79 0.0 0.0 0.0 9.015775766011997 0.0 80-81 0.0 0.0 0.0 9.684416943538473 0.0 82-83 0.0 0.0 0.0 10.370330857574434 0.0 84-85 0.0 0.0 0.0 11.103998807842501 0.0 86-87 0.0 0.0 0.0 11.857648943484284 0.0 88-89 0.0 0.0 0.0 12.547627030816594 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCACT 105 6.882908E-5 27.152884 1 CCGATAC 35 0.008397356 27.139082 32-33 TAAGGTA 70 0.008400953 27.134483 4 GTCCGCG 35 0.008414719 27.127594 10-11 GTAAGGT 95 0.0012280543 24.992289 4 AGTACTT 7395 0.0 24.362558 12-13 ATACTGA 215 1.6680133E-9 24.288658 6 ATGGGAT 1840 0.0 23.742674 5 GAGTACT 7360 0.0 23.57539 12-13 CGGGAGT 405 0.0 23.449554 4 GTACATG 9145 0.0 23.434021 1 CATGGGG 1645 0.0 23.381842 4 TACATGG 9135 0.0 23.30165 2 CATGGGT 475 0.0 22.992907 4 ATGGGAG 1635 0.0 22.94399 5 ACATGGG 9210 0.0 22.53105 3 GTCGGGA 425 0.0 22.359303 2 TCGGGAG 425 0.0 22.346046 3 ATGGGGG 750 0.0 22.15983 5 TACTGAT 150 3.0431811E-5 22.154198 7 >>END_MODULE