##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938450_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 597814 Sequences flagged as poor quality 0 Sequence length 101 %GC 41 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.70557397451381 31.0 31.0 34.0 30.0 34.0 2 31.850353119866714 31.0 31.0 34.0 30.0 34.0 3 31.87239007450478 31.0 31.0 34.0 30.0 34.0 4 35.59138628402814 37.0 35.0 37.0 33.0 37.0 5 35.498660118364576 37.0 35.0 37.0 33.0 37.0 6 35.58190674691459 37.0 35.0 37.0 33.0 37.0 7 35.507835212959215 37.0 35.0 37.0 33.0 37.0 8 35.53385333899842 37.0 35.0 37.0 33.0 37.0 9 37.02239994379523 39.0 37.0 39.0 33.0 39.0 10-11 36.9415277996166 39.0 37.0 39.0 33.0 39.0 12-13 36.81618781092447 39.0 37.0 39.0 32.0 39.0 14-15 37.60708681964624 39.0 36.5 41.0 32.0 41.0 16-17 37.60499419551901 39.0 36.5 41.0 32.0 41.0 18-19 37.8929391081507 39.5 37.0 41.0 33.0 41.0 20-21 37.996741461391004 40.0 37.0 41.0 33.0 41.0 22-23 37.87808498964561 40.0 37.0 41.0 32.5 41.0 24-25 37.732134911527666 40.0 36.5 41.0 32.0 41.0 26-27 37.434029648017614 39.0 36.0 41.0 32.0 41.0 28-29 37.22527910018835 39.0 36.0 41.0 31.5 41.0 30-31 36.82725898021793 38.5 35.0 40.0 31.0 41.0 32-33 36.39899868520978 38.0 35.0 40.0 30.5 41.0 34-35 36.10352718404052 38.0 35.0 40.0 30.0 41.0 36-37 35.90393834871716 38.0 35.0 40.0 30.0 41.0 38-39 35.64622441093718 38.0 35.0 40.0 30.0 41.0 40-41 35.32728574439541 38.0 35.0 40.0 28.0 41.0 42-43 34.96843750731833 38.0 34.0 40.0 26.5 41.0 44-45 34.456547521469886 38.0 33.5 40.0 24.5 41.0 46-47 33.706202096304196 37.5 33.0 40.0 21.5 41.0 48-49 33.10359911276751 37.0 33.0 40.0 16.0 41.0 50-51 32.696495063682015 37.0 33.0 40.0 10.0 41.0 52-53 32.15338382841486 36.0 31.0 40.0 7.5 41.0 54-55 31.869847477643546 36.0 31.0 39.5 3.5 41.0 56-57 31.58621159758721 35.0 31.0 39.0 2.0 41.0 58-59 31.191991154439343 35.0 30.5 39.0 2.0 40.0 60-61 30.81893866654177 35.0 29.5 39.0 2.0 40.0 62-63 30.34053652139294 35.0 29.0 38.0 2.0 40.0 64-65 29.800432910570848 35.0 28.0 38.0 2.0 40.0 66-67 29.320215819636207 34.0 28.0 37.0 2.0 40.0 68-69 28.86462009922819 34.0 26.5 37.0 2.0 39.0 70-71 28.332441194083778 34.0 26.0 36.0 2.0 39.0 72-73 27.941638201848736 33.0 26.0 35.5 2.0 38.5 74-75 27.514300434583333 33.0 26.0 35.0 2.0 38.0 76-77 26.234602234139714 31.5 23.5 34.5 2.0 36.0 78-79 26.902608503648292 33.0 25.0 35.0 2.0 36.0 80-81 26.684349981766907 33.0 25.0 35.0 2.0 36.0 82-83 26.276586864810795 33.0 24.0 35.0 2.0 36.0 84-85 25.821881889684754 32.5 22.0 35.0 2.0 35.0 86-87 25.277773688806217 32.0 20.0 35.0 2.0 35.0 88-89 24.644558340888636 31.5 17.0 34.0 2.0 35.0 90-91 24.12083189754673 31.0 9.0 34.0 2.0 35.0 92-93 23.48525962924923 31.0 2.0 34.0 2.0 35.0 94-95 22.852632256855813 31.0 2.0 34.0 2.0 35.0 96-97 21.95566932189611 30.0 2.0 34.0 2.0 35.0 98-99 20.880125758178966 29.0 2.0 34.0 2.0 35.0 100-101 17.70590350844912 22.5 2.0 31.5 2.0 34.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.40055965821607487 1101 2 0.5050785192242415 1101 3 0.48642946995606096 1101 4 0.38045175449558144 1101 5 0.32735345167301233 1101 6 0.4351216126094144 1101 7 0.43671844917469826 1101 8 0.42601877650739084 1101 9 0.43522809951014807 1101 10-11 0.4753200991995925 1101 12-13 0.5382176867440265 1101 14-15 0.568948599316677 1101 16-17 0.6634691334199729 1101 18-19 0.8013375871049533 1101 20-21 0.5855383349224752 1101 22-23 0.4818395036953831 1101 24-25 0.5019107493182986 1101 26-27 0.7569211655486541 1101 28-29 0.6223713803359558 1101 30-31 0.6136167149783205 1101 32-33 0.6713478916087183 1101 34-35 0.4492211874664491 1101 36-37 0.6238493531147 1101 38-39 0.7788925119294703 1101 40-41 0.9065334822115361 1101 42-43 0.7652211310041537 1101 44-45 0.649430275662894 1101 46-47 0.5291300804368788 1101 48-49 0.735298433218702 1101 50-51 1.013402408633823 1101 52-53 1.0207472596045406 1101 54-55 1.1565115769345233 1101 56-57 1.046500863650028 1101 58-59 0.9338366622156933 1101 60-61 0.6897755657160367 1101 62-63 0.8820437750947576 1101 64-65 0.5692359015070956 1101 66-67 0.7330204342853079 1101 68-69 0.6332314260091039 1101 70-71 0.3617231796161562 1101 72-73 0.3820011368383689 1101 74-75 0.5599852588178429 1101 76-77 0.6412608025708231 1101 78-79 0.8810907832597827 1101 80-81 0.8669580192539108 1101 82-83 1.0143919883689243 1101 84-85 1.0939256114422165 1101 86-87 1.2055540921557046 1101 88-89 1.03522140005445 1101 90-91 1.0759410241644716 1101 92-93 0.9839187593464374 1101 94-95 1.301808983705648 1101 96-97 1.0334411782005617 1101 98-99 0.6124010853329267 1101 100-101 0.47629787626444653 1102 1 0.38000193479359723 1102 2 0.4602750548671466 1102 3 0.39634666801449825 1102 4 0.32422733268155923 1102 5 0.3320408029626236 1102 6 0.3523910520307538 1102 7 0.4253450790404969 1102 8 0.3668439406836512 1102 9 0.25884568778462835 1102 10-11 0.4518595490901447 1102 12-13 0.6317624664147203 1102 14-15 0.654118010185627 1102 16-17 0.47786667791412185 1102 18-19 0.6824287644153202 1102 20-21 0.7933640701466089 1102 22-23 0.8001867959767139 1102 24-25 0.7999501516215304 1102 26-27 0.6130527920833373 1102 28-29 0.4837257155410555 1102 30-31 0.567920988319905 1102 32-33 0.4074880838615016 1102 34-35 0.5010080914352315 1102 36-37 0.461231574490931 1102 38-39 0.5223730487144707 1102 40-41 0.7575851620440304 1102 42-43 0.6104433638013376 1102 44-45 0.6252797093737499 1102 46-47 0.5918858622965857 1102 48-49 0.5678148508450462 1102 50-51 0.5427043026092662 1102 52-53 0.48398605279002993 1102 54-55 0.5014363889646027 1102 56-57 0.3467054891378005 1102 58-59 0.6776622070905383 1102 60-61 0.7534711971998114 1102 62-63 1.014526881595188 1102 64-65 0.9562160100061874 1102 66-67 0.7629885507790739 1102 68-69 0.775398552686557 1102 70-71 0.33591895386189563 1102 72-73 0.25574007278583366 1102 74-75 0.5647439910915644 1102 76-77 0.5144524843068119 1102 78-79 0.7236243323223128 1102 80-81 0.8606883894832826 1102 82-83 0.5965158105826838 1102 84-85 0.5453151612661458 1102 86-87 0.6746223323765079 1102 88-89 0.8770173456145507 1102 90-91 0.6763978624627498 1102 92-93 0.7209036265778082 1102 94-95 0.8687881404582853 1102 96-97 0.7651319557774201 1102 98-99 0.2524862666406271 1102 100-101 -0.3225624073559956 1103 1 0.14161014539659433 1103 2 0.2559898885566696 1103 3 0.2871545446749444 1103 4 0.06361353595458041 1103 5 0.20250590499883714 1103 6 0.11598486700208355 1103 7 0.19782576291358822 1103 8 -0.10449534301601915 1103 9 0.28985463912120935 1103 10-11 0.3405915666382384 1103 12-13 0.2665240810720988 1103 14-15 0.5554405645238134 1103 16-17 0.5761669876812974 1103 18-19 0.37608159954245934 1103 20-21 0.38881490870988955 1103 22-23 0.36778598755208236 1103 24-25 0.11180446757115448 1103 26-27 0.4150387187900435 1103 28-29 0.4382647233190795 1103 30-31 0.4656817036532104 1103 32-33 0.6309846171123112 1103 34-35 0.6214482122476497 1103 36-37 0.5039188450262984 1103 38-39 0.6916925601784385 1103 40-41 0.9681849370518378 1103 42-43 0.9674814609734668 1103 44-45 1.1377332523559645 1103 46-47 0.975434678831256 1103 48-49 1.4001457118430736 1103 50-51 1.276997559612873 1103 52-53 1.3767408532142298 1103 54-55 1.4829744587379672 1103 56-57 1.8229104705549908 1103 58-59 1.672375857798226 1103 60-61 1.2425713251681394 1103 62-63 1.3079950168354735 1103 64-65 1.1898114044355381 1103 66-67 1.3305587319010037 1103 68-69 1.4089239996892928 1103 70-71 0.9781120796737888 1103 72-73 0.9513204913744424 1103 74-75 1.0933708618628906 1103 76-77 0.9292987450205317 1103 78-79 0.9507971346998794 1103 80-81 1.0383741933790667 1103 82-83 1.2653485911057096 1103 84-85 1.095235704857572 1103 86-87 1.063923277150277 1103 88-89 1.0467978009497365 1103 90-91 0.6883276055913008 1103 92-93 1.0949047805491787 1103 94-95 0.9656443743212222 1103 96-97 0.9800607351142361 1103 98-99 0.4454412391935172 1103 100-101 -0.1354772165477982 1104 1 0.2401586557098092 1104 2 0.3898798081608703 1104 3 0.2690109235901481 1104 4 0.178786878018677 1104 5 0.1786781920954965 1104 6 0.24974746294954997 1104 7 0.3361992941442651 1104 8 0.3014215692034199 1104 9 0.416341597540935 1104 10-11 0.4085460211473162 1104 12-13 0.15802613643969465 1104 14-15 0.47415805151861434 1104 16-17 0.4298493697250265 1104 18-19 0.3962623991350256 1104 20-21 0.44773847813765855 1104 22-23 0.5308011936345167 1104 24-25 0.504193241262449 1104 26-27 0.4437274670166005 1104 28-29 0.6295321393764155 1104 30-31 0.5097857088916768 1104 32-33 0.518957988278963 1104 34-35 0.6267185021818094 1104 36-37 0.7378396860899983 1104 38-39 0.6981997084923108 1104 40-41 0.8796358810658091 1104 42-43 0.9189984313979949 1104 44-45 0.6962884926338901 1104 46-47 0.8696241728106671 1104 48-49 0.8317001518008738 1104 50-51 0.9329781336606757 1104 52-53 0.8996409223327859 1104 54-55 1.090722103250311 1104 56-57 0.7962770899300189 1104 58-59 0.586718867729477 1104 60-61 0.7403931811832756 1104 62-63 0.7950853074971924 1104 64-65 0.7435556293982515 1104 66-67 0.6930992026347518 1104 68-69 0.35655759858339664 1104 70-71 0.4007493163866549 1104 72-73 0.15236454535967425 1104 74-75 0.10111486855647556 1104 76-77 0.07408393182252482 1104 78-79 0.055052473198916374 1104 80-81 0.2350210339334673 1104 82-83 -0.13678953687275452 1104 84-85 -0.22101352210593816 1104 86-87 -0.19741762284608555 1104 88-89 -0.27112482264515947 1104 90-91 -0.15539624758633508 1104 92-93 -0.11476545117987769 1104 94-95 -0.32386233745439696 1104 96-97 -0.5243819510511365 1104 98-99 -0.6859340381901653 1104 100-101 -0.9455603407065531 1105 1 0.43574518300365384 1105 2 0.34615988116568275 1105 3 0.372785604194096 1105 4 0.1098636989909707 1105 5 0.05775567674789528 1105 6 0.18314716429350142 1105 7 0.17461807629126724 1105 8 0.33185959807349974 1105 9 0.16261669294495107 1105 10-11 0.398176019488119 1105 12-13 0.39726622271810896 1105 14-15 0.6138336778667082 1105 16-17 0.43296451822345006 1105 18-19 0.39597203908856926 1105 20-21 0.37628501870510433 1105 22-23 0.5624296986950768 1105 24-25 0.6084698299466993 1105 26-27 0.5788402926346592 1105 28-29 0.6674199774384704 1105 30-31 0.810992777084806 1105 32-33 0.7809954862049651 1105 34-35 0.4786805064957278 1105 36-37 0.5357739817594833 1105 38-39 0.6813712697997545 1105 40-41 0.5484553600769289 1105 42-43 0.7268049686470448 1105 44-45 0.6391331914890443 1105 46-47 -0.16022898796583718 1105 48-49 -0.2697599444327423 1105 50-51 -0.05644446464694397 1105 52-53 0.10020505042304961 1105 54-55 -0.3288771998855786 1105 56-57 -0.013383603615693573 1105 58-59 0.07118460524399595 1105 60-61 0.19938189862147127 1105 62-63 -0.08335753305366111 1105 64-65 0.13079223135610718 1105 66-67 -0.044485831378853646 1105 68-69 0.047813613184356285 1105 70-71 -0.40884500914843613 1105 72-73 -0.028471691574125657 1105 74-75 0.026878100678590755 1105 76-77 -0.0499412880215111 1105 78-79 0.07616994457771042 1105 80-81 5.801844229331721E-4 1105 82-83 0.012642187036323804 1105 84-85 -0.09404736979135464 1105 86-87 0.04142425536861438 1105 88-89 0.08927421501868693 1105 90-91 0.3546377660191098 1105 92-93 0.5630800772754085 1105 94-95 0.6982299140803647 1105 96-97 0.5187199238451612 1105 98-99 0.8064211965866726 1105 100-101 0.5723963541665213 1106 1 0.04651314426063635 1106 2 0.02163300860910411 1106 3 0.12922406042558165 1106 4 -0.03388587827186029 1106 5 -0.15384017298764263 1106 6 -0.21789716561735162 1106 7 -0.0036566149414980487 1106 8 0.056481163756714636 1106 9 -0.09660168293265059 1106 10-11 -0.17547494164711708 1106 12-13 -0.09517997129457711 1106 14-15 0.08565165062214675 1106 16-17 -0.11884073859783228 1106 18-19 0.10062417712408944 1106 20-21 -0.024793130179546097 1106 22-23 -0.1315288798258223 1106 24-25 0.004112593362691541 1106 26-27 0.029832541325866657 1106 28-29 0.12844834479368927 1106 30-31 0.23740945389716472 1106 32-33 0.1484021120183101 1106 34-35 0.01415525724988953 1106 36-37 -0.34794421149565835 1106 38-39 -0.09754462917620543 1106 40-41 0.01362316982491052 1106 42-43 0.3197449196771771 1106 44-45 0.0893706006850934 1106 46-47 -0.2375229289584908 1106 48-49 0.045561403456275684 1106 50-51 -0.17837539277485348 1106 52-53 0.11961443586465492 1106 54-55 -0.22758666784548254 1106 56-57 -0.031154797966571834 1106 58-59 -0.1930430370324281 1106 60-61 -1.003914774944267 1106 62-63 -0.7553337786064667 1106 64-65 -1.044146556301321 1106 66-67 -0.711318887396601 1106 68-69 -0.9701145012049359 1106 70-71 -0.8319311934212621 1106 72-73 -0.5881472999774644 1106 74-75 -0.19838737826571418 1106 76-77 -0.2694534831434616 1106 78-79 -0.8776008710006629 1106 80-81 -1.5559664925246999 1106 82-83 -1.0469265534098184 1106 84-85 -0.7025508125628868 1106 86-87 -0.7415390983913781 1106 88-89 -0.4923986067012116 1106 90-91 -0.48723688733640813 1106 92-93 -0.4897654511798777 1106 94-95 -0.7959072286221769 1106 96-97 -0.810657942312865 1106 98-99 -1.006271740658839 1106 100-101 -1.0750936015982617 1107 1 0.008780838125094448 1107 2 0.07678403693702052 1107 3 -0.05346257991783787 1107 4 0.25754063657539206 1107 5 0.061102008016845843 1107 6 0.14705622971644772 1107 7 0.18058438360324658 1107 8 -0.009099190824734649 1107 9 0.06657724038741719 1107 10-11 0.01557158999554531 1107 12-13 -5.636317559876147E-4 1107 14-15 0.12190131810424276 1107 16-17 0.1642793486131353 1107 18-19 0.12107207222055649 1107 20-21 0.3473556917942773 1107 22-23 0.2794123936003956 1107 24-25 0.4459675999087267 1107 26-27 0.256116842829627 1107 28-29 0.2732422880126677 1107 30-31 -0.028724836392271413 1107 32-33 0.0833172743131243 1107 34-35 0.324026735820091 1107 36-37 0.2607109035424955 1107 38-39 0.23935990297762544 1107 40-41 0.36249313054867827 1107 42-43 0.3464076395953839 1107 44-45 0.33224428560938435 1107 46-47 0.13381872210374013 1107 48-49 0.5096638137546847 1107 50-51 -0.044134655028472025 1107 52-53 -0.1655020627609396 1107 54-55 0.09178569483114885 1107 56-57 0.25801711509368985 1107 58-59 0.31009175920581455 1107 60-61 0.05279321956853522 1107 62-63 0.21368989666828497 1107 64-65 0.2575475898187847 1107 66-67 0.14652775273818008 1107 68-69 0.054231025320863324 1107 70-71 0.1673216192890088 1107 72-73 0.11011943414904124 1107 74-75 0.19015001239457163 1107 76-77 0.22114212814180334 1107 78-79 0.13863904262292337 1107 80-81 0.15632858516712744 1107 82-83 0.15828915291036694 1107 84-85 0.4147236881386611 1107 86-87 0.5268589216275004 1107 88-89 0.18863196408207372 1107 90-91 0.28295542537124874 1107 92-93 0.12622200963517116 1107 94-95 0.058950662353677075 1107 96-97 0.04077436225788489 1107 98-99 -0.009257881834205506 1107 100-101 -0.9383231197993815 1108 1 0.17966959522074788 1108 2 0.16975391996775357 1108 3 0.1476353463322191 1108 4 0.06120471001634087 1108 5 0.01032977164623361 1108 6 0.08144230304775135 1108 7 0.11443220893180239 1108 8 -0.020282788215226333 1108 9 0.09233091218571587 1108 10-11 0.09573441001637661 1108 12-13 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-0.6006915731184073 2316 58-59 -0.7474753853994578 2316 60-61 -0.6243154549928143 2316 62-63 -0.8629602590964751 2316 64-65 -0.5196332386839728 2316 66-67 -0.6898461216847096 2316 68-69 -0.7584856193258496 2316 70-71 -0.7221426864486951 2316 72-73 -0.5988759197221611 2316 74-75 0.1136107689384005 2316 76-77 -0.25701180351671127 2316 78-79 -0.353172947607046 2316 80-81 -0.24130301045878255 2316 82-83 -0.5781795454422785 2316 84-85 -0.4091000319820495 2316 86-87 -0.012818433248007466 2316 88-89 -0.0029952535546904357 2316 90-91 -0.36319530811256584 2316 92-93 -0.45710604215965844 2316 94-95 -0.7894017413963788 2316 96-97 -0.3818831941611336 2316 98-99 -0.5250610575826791 2316 100-101 -0.45671262432606596 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 6.0 4 16.0 5 64.0 6 168.0 7 326.0 8 570.0 9 1017.0 10 1648.0 11 2576.0 12 3455.0 13 5001.0 14 5986.0 15 7912.0 16 10240.0 17 13327.0 18 11134.0 19 6921.0 20 5942.0 21 6423.0 22 6655.0 23 7565.0 24 8658.0 25 9689.0 26 11325.0 27 13181.0 28 15964.0 29 18821.0 30 21766.0 31 25935.0 32 30477.0 33 37418.0 34 48753.0 35 66356.0 36 72434.0 37 78754.0 38 39512.0 39 1817.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 47.97025810876365 18.807647914889348 14.973152004817583 18.248941971529415 2 15.65896454883531 23.553719008264462 38.858372747691206 21.92894369520902 3 20.390793715049455 30.193757537942723 30.449190293220745 18.96625845378708 4 12.042207107896436 17.93718447543885 35.976072825326945 34.04453559133777 5 11.8075076890107 41.41886314816644 31.045186436534898 15.72844272628796 6 27.442816662038695 36.79422027587176 18.02567353725406 17.737289524835482 7 25.905381941540345 37.96214207094514 18.78527434954685 17.347201637967665 8 27.522440090061455 32.456081657505514 19.301488422820476 20.719989829612555 9 29.692011227572458 16.547287283335617 19.48850311300839 34.27219837608353 10-11 24.91259823289518 28.561392004871077 24.304047747292636 22.221962014941102 12-13 28.918693774317767 26.6813758125437 25.978899791573966 18.421030621564565 14-15 24.983523303234787 26.06722157727989 28.593843570073634 20.355411549411688 16-17 19.299564747563625 30.489583716674417 28.9476325412252 21.26321899453676 18-19 19.21684671151897 27.46138431017005 34.915876844637296 18.405892133673685 20-21 21.24732776415407 25.801838029888895 34.25655805986477 18.69427614609226 22-23 20.218579692011225 26.42795250696705 33.60418123362785 19.749286567393874 24-25 19.91141042433002 26.587662028092396 34.65414770170605 18.846779845871538 26-27 20.19467549066187 26.104951117832453 35.130837588078236 18.56953580342744 28-29 19.02899564078459 26.447858363972742 35.968294486244886 18.554851508997782 30-31 18.850093841897312 27.904498723683286 34.92223333679037 18.32317409762903 32-33 18.452980358439248 27.993238699662438 34.38368790292633 19.170093038971988 34-35 18.90867784633092 27.225672185120253 34.42807385002476 19.43757611852407 36-37 19.815059224205562 27.032031755749706 34.3432645325545 18.809644487490235 38-39 20.22715600811666 26.503262889710815 34.68984564632985 18.579735455842673 40-41 19.04806115962394 27.40171969620931 34.786794472882995 18.763424671283747 42-43 18.653204229092278 28.186727756157246 34.00612065843497 19.15394735631549 44-45 18.87434887774458 28.55930105350494 32.83630025392513 19.73004981482535 46-47 19.06964373534243 28.32938004128374 31.787395410612667 20.81358081276116 48-49 19.561435496659495 28.179082457085315 31.600882548752622 20.658599497502568 50-51 20.08500971874195 28.954909051979378 31.027125493882714 19.93295573539596 52-53 19.37383534008906 29.256926067305216 31.038751183478475 20.330487409127254 54-55 18.30109365120255 29.115159564680653 31.293596335984102 21.290150448132696 56-57 19.45053143619922 29.713589845671063 29.798649747245797 21.03722897088392 58-59 19.24218904207663 28.689776418752324 30.43613899975578 21.631895539415268 60-61 19.398759480373496 28.51095825791969 30.86511858203388 21.225163679672942 62-63 20.548615455643393 28.302448587687813 30.49117283971269 20.65776311695611 64-65 19.796792982432663 28.568835791734553 31.033231071871853 20.60114015396093 66-67 19.147427126162984 28.755766843867825 30.95812409879996 21.138681931169227 68-69 19.266454056775707 29.30729489460254 30.561375423735193 20.864875624886565 70-71 19.082774909921817 29.464013890608115 29.588801868139587 21.86440933133048 72-73 19.298561090907874 28.770988969813356 29.311207164770316 22.619242774508457 74-75 19.15010354391165 29.04799820680011 29.65035947635887 22.15153877292937 76-77 19.181384176349166 28.18870083336958 30.821375879454145 21.808539110827112 78-79 18.827846119361542 28.157671115096 31.00747055104096 22.0070122145015 80-81 18.29895074919602 29.37584423079336 30.376835352517322 21.9483696674933 82-83 18.18005416445721 28.940849000265974 29.617319244106394 23.261777591170425 84-85 18.497660514469345 27.922684059881465 29.564506064930136 24.01514936071906 86-87 18.874236992701558 27.870070040264373 29.873251711695737 23.382441255338332 88-89 19.2309815427541 27.16028731344532 30.163897131883832 23.444834011916747 90-91 18.471617802409018 27.406027646578135 30.56530201381576 23.557052537197084 92-93 17.809952677192882 28.081460801025077 30.177615258385202 23.930971263396838 94-95 17.771330213076308 28.009799034482295 29.82993037968331 24.388940372758082 96-97 17.967210537056676 27.38217907242052 29.355200781513986 25.29540960900882 98-99 18.47146436851596 27.016095307236032 29.32291649242072 25.189523831827294 100-101 18.70205448517432 26.9328754428635 29.72722284857832 24.63784722338386 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 3442.0 1 2310.5 2 1667.0 3 2152.5 4 1754.5 5 1650.5 6 3201.5 7 6594.0 8 9236.5 9 8942.5 10 6313.5 11 4138.0 12 3692.5 13 3491.5 14 3296.0 15 3033.5 16 2774.5 17 2708.5 18 2642.0 19 2553.5 20 2586.5 21 2683.5 22 2794.5 23 3053.0 24 3275.0 25 3543.5 26 4018.5 27 4389.5 28 4850.5 29 5596.5 30 6115.0 31 6368.0 32 6903.5 33 7714.5 34 8691.5 35 9309.0 36 9944.5 37 10994.0 38 11884.5 39 12263.0 40 12727.5 41 13798.0 42 14833.0 43 15645.5 44 16498.5 45 17090.0 46 17928.5 47 20733.5 48 32195.0 49 33519.5 50 25473.0 51 22997.0 52 18873.5 53 16490.0 54 15074.5 55 14116.0 56 13285.0 57 12135.5 58 11083.5 59 10198.0 60 9114.5 61 8070.0 62 6736.0 63 4747.5 64 3272.0 65 2500.5 66 1878.5 67 1433.5 68 1043.5 69 727.5 70 564.0 71 454.0 72 387.5 73 380.5 74 332.5 75 228.0 76 146.5 77 95.0 78 66.0 79 43.0 80 23.5 81 11.5 82 6.5 83 3.0 84 1.0 85 0.5 86 0.0 87 0.0 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 6.69104437166075E-4 2 0.05285925053611993 3 8.363805464575939E-4 4 0.0 5 0.0891581662523795 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 1.6727610929151876E-4 26-27 0.008363805464575939 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010371118776074164 36-37 1.6727610929151876E-4 38-39 0.005185559388037082 40-41 0.0056873877159116385 42-43 4.1819027322879693E-4 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 9.200186011033533E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.001087294710394872 82-83 8.363805464575939E-4 84-85 0.00552011160662012 86-87 0.0025091416393727816 88-89 0.0 90-91 0.003094608021893097 92-93 8.363805464575939E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 597814.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 43.47502596463735 #Duplication Level Percentage of deduplicated Percentage of total 1 72.28040800494405 31.42392614749524 2 13.709446389511355 11.920370754896197 3 5.444231748456501 7.100643498649481 4 2.767835358249873 4.813268562630182 5 1.5672571128474482 3.406827183715269 6 0.9747137261586418 2.5425422731701226 7 0.6778317079765916 2.0628125772755768 8 0.4782150086852441 1.663232793541621 9 0.34764533169406303 1.360250083969592 >10 1.5814314439704296 11.873112716853273 >50 0.09242387450247225 2.8128586598667407 >100 0.06431161240959288 5.357674525013842 >500 0.005776492132598163 1.6738861254675914 >1k 0.00731689003462434 6.891930621611661 >5k 7.701989510130885E-4 2.988484430845819 >10k+ 3.8509947550654426E-4 2.1081790449977906 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12592 2.106340768198804 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9638 1.612207141351658 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8212 1.373671409501952 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 4973 0.8318640915067229 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 4759 0.7960670041183378 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 4147 0.6936940252319284 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 2865 0.4792460531202013 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2710 0.45331825618001587 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2132 0.356632665009518 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1911 0.31966464485609236 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 1855 0.31029718273576734 No Hit TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1835 0.30695166054993694 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1784 0.2984205789760695 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 1693 0.2831984530305413 No Hit GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA 1559 0.26078345438547773 No Hit GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1555 0.2601143499483117 No Hit CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT 1457 0.24372129123774286 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 1457 0.24372129123774286 No Hit ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1226 0.20508050999140198 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 1125 0.18818562295295863 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1086 0.18166185469058937 No Hit TATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGG 1036 0.17329804922601344 No Hit GGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGT 860 0.14385745399070612 No Hit ATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTG 820 0.1371664096190454 No Hit ACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCA 777 0.12997353691951008 No Hit CAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAG 764 0.12779894749872034 No Hit GGTCGGGAGTGGGTAATTTGCGCGCCTGCTGCCTTCCTTGGATGTGGTAG 739 0.12361704476643237 No Hit GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG 707 0.11826420926910376 No Hit ACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGT 695 0.11625689595760555 No Hit GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 659 0.11023495602311087 No Hit CTTATACACATCTCCGAGCCCACGAGACTCCTGAGCATCTCGTATGCCGT 636 0.10638760550940593 No Hit GTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCA 615 0.10287480721428405 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.6727610929151876E-4 0.0 20-21 0.0 0.0 0.0 5.018283278745563E-4 0.0 22-23 0.0 0.0 0.0 0.002341865530081263 0.0 24-25 0.0 0.0 0.0 0.007611062972764104 0.0 26-27 0.0 0.0 0.0 0.0164766967652146 0.0 28-29 0.0 0.0 0.0 0.036968020153425644 0.0 30-31 0.0 0.0 0.0 0.0838053307550509 0.0 32-33 0.0 0.0 0.0 0.15749045689796493 0.0 34-35 0.0 0.0 0.0 0.2685617934675334 0.0 36-37 0.0 0.0 0.0 0.4318232761360557 0.0 38-39 0.0 0.0 0.0 0.6624133927944142 0.0 40-41 0.0 0.0 0.0 0.9221095524694972 0.0 42-43 0.0 0.0 0.0 1.1496886991606083 0.0 44-45 0.0 0.0 0.0 1.4026938144640306 0.0 46-47 0.0 0.0 0.0 1.7137437396916098 0.0 48-49 0.0 0.0 0.0 2.0591689053785958 0.0 50-51 0.0 0.0 0.0 2.4164706748252804 0.0 52-53 0.0 0.0 0.0 2.7438300207087822 0.0 54-55 0.0 0.0 0.0 3.071607556865513 0.0 56-57 0.0 0.0 0.0 3.428742050202906 0.0 58-59 0.0 0.0 0.0 3.8660854379455816 0.0 60-61 0.0 0.0 0.0 4.398358017711194 0.0 62-63 0.0 0.0 0.0 4.87752043277675 0.0 64-65 0.0 0.0 0.0 5.295292515732318 0.0 66-67 0.0 1.6727610929151876E-4 0.0 5.745767078054378 0.0 68-69 0.0 1.6727610929151876E-4 0.0 6.252111860879806 0.0 70-71 0.0 1.6727610929151876E-4 0.0 6.824028878547509 0.0 72-73 0.0 1.6727610929151876E-4 0.0 7.405982462772702 0.0 74-75 0.0 1.6727610929151876E-4 0.0 7.944778810800684 0.0 76-77 0.0 1.6727610929151876E-4 0.0 8.489178908489933 0.0 78-79 0.0 1.6727610929151876E-4 0.0 9.078659917633244 0.0 80-81 0.0 1.6727610929151876E-4 0.0 9.720665625094092 0.0 82-83 0.0 1.6727610929151876E-4 0.0 10.40315215100349 0.0 84-85 0.0 1.6727610929151876E-4 0.0 11.128712275055452 0.0 86-87 0.0 1.6727610929151876E-4 0.0 11.889567658167925 0.0 88-89 0.0 1.6727610929151876E-4 0.0 12.587610862241434 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTTAGG 80 4.453787E-4 29.729803 1 ATCGTGC 65 0.0058442215 29.228352 8 GTATAGA 135 4.1671592E-7 28.188255 1 GGTTGCA 85 6.406655E-4 27.938864 8 ATACGAG 35 0.00839509 27.14061 72-73 CGTCACT 90 8.868456E-4 26.42649 1 TCGACCG 45 8.952083E-4 26.386705 18-19 GTATAGG 115 1.2684235E-4 24.817923 1 CTACACT 155 1.4005436E-6 24.532566 4 ATGGGGA 630 0.0 23.388689 5 GAGTACT 7530 0.0 22.864973 12-13 AGTACTT 7550 0.0 22.55277 12-13 CATGGGG 1690 0.0 22.500284 4 TACCTGG 425 0.0 22.38479 2 GTACATG 10025 0.0 22.20616 1 GGGATAA 215 4.4165063E-8 22.091194 7 TACATGG 10030 0.0 21.957962 2 ATGGGAG 1520 0.0 21.889666 5 CCTAGAC 110 0.002852101 21.621674 2 CGACCGT 55 0.0028788152 21.589123 18-19 >>END_MODULE